KR102234653B1 - Manufacturing method of bamboo salt water with enhanced anti-fatigue after aerobic exercise - Google Patents

Abstract

The present invention relates to a method for manufacturing bamboo salt water by immersing herbal medicines such as Artemisia, corydaline, bellflower leaves, and S. palmata in bamboo salt mineral water fortified with calcium at low temperature and adding monellin and thaumatin, wherein the bamboo salt mineral water has an effect of inhibiting cortisol, phosphokinase or lactic acid secretion, and can be easily used as a sports drink composition or ionic drink composition for relieving fatigue after aerobic exercise, a drink composition for enhancing vitality of rehabilitation patients, and the like by increasing glucose consumption.

Description

Manufacturing method of bamboo salt water with enhanced anti-fatigue after aerobic exercise

The present invention prepares bamboo salt water with improved refreshing feeling and enhanced fatigue relief effect after aerobic exercise by immersing herbal medicines such as aeropsis, hyeonhosaek, bellflower leaves, and Jeju joritdae in calcium-enriched bamboo salt mineral water and adding monelin and taumartin to relieve fatigue after aerobic exercise. It's about how to do it.

Fatigue can sometimes be defined as'the perception of a reduced capacity for physical and mental activity due to an imbalance in the availability, utilization and recovery of resources needed to carry out an activity', specifically, fatigue is primarily physical. Workability is reduced due to fatigue, and stress refers to a state in which acidic confusion occurs due to mental fatigue (establishment of a functional evaluation system related to fatigue recovery of health functional foods from the KFDA, research report). Fatigue is classified into central nervous system fatigue, nerve-muscle junction fatigue, and peripheral fatigue of the body. Fatigue is a comprehensive physiological process including various physiological and biochemical factors, and is a mental physiological phenomenon that inevitably appears when the body's mental or physical activity reaches a certain stage. This means that the human body's original work ability is temporarily deteriorated, and may be a symptom indicating that the body develops into a damaged state.

If fatigue continues to occur, a chronic condition may develop into a disease called chronic fatigue syndrome. Currently, the main symptom of Chronic Fatigue Syndrome (CFS), long-lasting fatigue is a group of non-specific symptoms such as mild fever, headache, sore throat, muscle joint pain, attention-focusing disorder, memory loss, sleep disturbance and depression. It is a syndrome, and there is generally no obvious abnormality in the case of physical examination and regular examination (Holmes GO, er al, Amm. Intern Med, 1988, 108 (3)). There are various causes of fatigue such as lack of sleep, lack of exercise, unbalanced diet, bad habits such as alcoholism, mental pressure, bad work environment, and work environment.

There are several approaches to relieving fatigue, including rest, finding a satisfying job, regular exercise, changing to a better diet, and getting enough sleep. However, it is difficult to relieve physical and physiological fatigue in a short time by performing a specific method of relieving fatigue. Therefore, there is a need to develop a product that helps relieve physical and mental fatigue, especially to overcome immunity decline, disorders of the nervous and endocrine systems, abnormal vascular and digestive disorders, but there is no development of a product with related effects. Drugs with the same arousal effect are used as drugs to improve fatigue, but they do not actually reduce blood fatigue substances.

The present inventors have completed the present invention by confirming that a sports drink composition using bamboo salt has excellent stress relief or anti-fatigue efficacy while researching a composition capable of enhancing various physiological activities.

Korean Patent Registration No. 10-0893203 (Name of invention: pharmaceutical composition using distilled water containing bamboo salt as an active ingredient, Applicant: Eun-ah Choi, registration date: April 6, 2009) Republic of Korea Patent Registration No. 10-1281237 (Name of invention: bamboo salt paper longevity and its manufacturing method, Applicant: Kim Kwang-hoe, registration date: June 26, 2013)

The object of the present invention is to immerse herbal medicines such as aeropsis, hyeonhosaek, bellflower leaves, and Jeju joritdae in calcium-enriched bamboo salt mineral water, and add monelin and taumatin, thereby enhancing the effect of relieving fatigue after aerobic exercise and improving the refreshing feeling. It is to provide a method of manufacturing.

The present invention relates to a method of manufacturing bamboo salt water for relieving fatigue after aerobic exercise, wherein the bamboo salt water may be prepared including the following steps.

Preferably, (1st step) preparing bamboo salt containing CaO by mixing the sea salt with bamboo and oyster shells and firing at 900 to 1200° C. for 6 to 24 hours;

(Second step) The bamboo salt is suspended in mineral water so that its salinity is 0.8 to 1.0% (w/v), stirred at 4 to 10°C for 1 to 4 hours at 10 to 30 rpm, and then insoluble substances are removed to remove calcium (Ca ^{2 +} ) Preparing bamboo salt mineral water with enhanced content;

(3rd step) Into the bamboo salted water obtained in the second step, add aeop, hyeonho color, bellflower leaves, and jeju joritdae, stir at 4 to 10°C for 18 to 24 hours at 10 to 30 rpm, and then filter to prepare a herbal medicine leaching bamboo salt water. The step of doing;

(Step 4) Pasteurizing by maintaining the herbal medicine leaching bamboo salt water obtained in the third step at 62 to 65°C for 30 to 40 minutes; And,

(Step 5) adding monelin and taumatin to the herbal medicine leaching bamboo salt water after pasteurization, sterilizing with ultraviolet rays, and encapsulating in a sterilization pack;

It may contain.

It is preferable to mix the sea salt, bamboo and oyster shells of the first step in a weight ratio of 1:0.5:0.5 to 1:2:2.

In the third step, based on 100 parts by weight of bamboo salt mineral water, 3 to 5 parts by weight of aeyeop, 3 to 5 parts by weight of hyeonho color, 5 to 7 parts by weight of bellflower leaves, and 2 to 4 parts by weight of Jeju joritdae may be added.

In the fifth step, it is preferable to add 0.5 to 1 parts by weight of monelin and 0.5 to 1 parts by weight of taumatin based on 100 parts by weight of herbal leaching bamboo salt water.

The present invention provides bamboo salt water for relieving fatigue after aerobic exercise prepared by the above method.

The bamboo saline solution is characterized in that it has the effect of inhibiting the secretion of cortisol, phosphokinase or lactic acid.

The bamboo saline solution has the effect of enhancing the amount of glucose consumed in the blood.

Monellin and taumatin used in the present invention may be purified from hot water extracts of plants to be extracted, and UV irradiated and ozone sterilized in a final aqueous solution before drying. It is also better to use lyophilized powder.

The monelin is a sweetener contained in the fruit of Dioscoreophyllum cummisii , and the taumartin is a sweetening component contained in the fruit of Thaumatococcus danielli.

The present invention relates to a method for preparing bamboo salt water by immersing herbal medicines such as aerobium, hyeonhosaek, bellflower leaf, and Jeju joritdae in calcium-enriched bamboo salt mineral water, and adding monelin and taumatin to prepare bamboo salt water, wherein the bamboo salt water is cortisol, pos While having the effect of inhibiting the secretion of pokinase or lactic acid, it can be easily used as a sports drink composition or ionic drink composition for relieving fatigue after aerobic exercise by enhancing the amount of glucose consumption, and a beverage composition for enhancing vitality of rehabilitation patients.

Hereinafter, a preferred embodiment of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided to sufficiently convey the spirit of the present invention to those skilled in the art so that the contents introduced herein are thorough and complete.

<Examples 1 to 5. Preparation of bamboo salt water>

Example 1

Natural sea salt, bamboo (bamboo pieces cut into 2~5cm) and oyster shells were mixed at the same weight and fired at 100℃ for 12 hours to produce bamboo salt containing CaO (previously, only sea salt was put in a bamboo barrel and fired several times. To manufacture bamboo salt).

The bamboo salt was dissolved in mineral water and the salinity (NaCl concentration) was adjusted to 0.9% (w/v). After that, the mixture was stirred at 4° C. for 1 hour at 20 rpm to ensure that the water-soluble components calcined from the oyster shell, including calcium, were sufficiently dissolved in bottled water. Next, the supernatant obtained by centrifugation at 10000 rpm for 30 minutes was filtered by sequentially applying a nonwoven fabric filter, a filter paper filter, and a filter having 0.2 μm pores.

Based on 100 g of the bamboo salt water obtained by filtration, 4 g of aeyeop, 4 g of Hyeonho color, 6 g of bellflower leaves, and 3 g of Jeju joritdae were added, and the mixture was stirred at 4°C for 24 hours at 20 rpm, and then the ingredients were removed to prepare a herbal medicine leached bamboo salt water. Thereafter, the crude drug leached bamboo salt water was kept at 65° C. for 30 minutes to pasteurize, and after pasteurization, monelin 1 g and taumatin 0.5 g were added based on 100 g of the crude drug leached bamboo salt water, and ultraviolet sterilization was enclosed in a sterilization pack.

Example 2

Bamboo salt water was prepared in the same manner as in Example 1, but the weight of the raw material during the manufacturing process was 3g of aeyeop, 5g of Hyeonho color, 5g of bellflower leaves, and 4g of Jeju joritdae.

Example 3

Bamboo salt water was prepared in the same manner as in Example 1, but the weight of raw materials during the manufacturing process was put in 5 g of aeyeop, 3 g of hyeonho color, 7 g of bellflower leaves, and 2 g of Jeju joritdae.

Example 4

Bamboo salt water was prepared in the same manner as in Example 1, but the weight of the raw material during the manufacturing process was 0.5 g of monelin and 1 g of taumatin were added.

Comparative Example 1

Bamboo salt water was prepared in the same manner as in Example 1, but the weight of the raw materials during the manufacturing process was adjusted to 1 g of aeropsis, 7 g of hyeonho saek, 1 g of bellflower leaves, 8 g of Jeju joritdae, 1 g of monelin, and 0.5 g of taumatin.

Comparative Example 2

Bamboo salt water was prepared in the same manner as in Example 1, but the weight of the raw material during the manufacturing process was 10 g of aerobium, 13 g of hyeonho color, and no bellflower leaves and jeju joritdae were added, and 1.5 g of monelin was added and no taumatin was added.

Comparative Example 3

Bamboo saline was prepared in the same manner as in Example 1, but 1.5g of enzyme-treated stevia extract (steviol glycoside 95% or more) was added instead of monelin and taumartin.

Comparative Example 4

Bamboo saline was prepared in the same manner as in Example 1, but 0.2 g of monelin and 2 g of taumatin were added.

Comparative Example 5

Bamboo saline was prepared in the same manner as in Example 1, but 1.5 g of oligosaccharide was added instead of monelin and taumartin.

Comparative Example 6

Bamboo salt water was prepared in the same manner as in Example 1, but high-temperature and high-pressure sterilization was performed at 121° C. and 1.5 atmospheres for 15 minutes instead of pasteurization.

Comparative Example 7

Bamboo salt water was prepared in the same manner as in Example 1, but bamboo salt water was used using commercially available bamboo salt (made by putting the sea salt in a bamboo barrel) instead of bamboo salt prepared using natural sea salt and bamboo and oyster bark.

Comparative Example 8

In 0.9% (w/v) salt water prepared with sea salt, aeop, hyeonho saek, bellflower leaves, and jeju joritdae were added in the same amount as in Example 1, and a beverage composition was prepared according to the method of Example 1.

Comparative Example 9

In a commercially available general physiological saline solution having a salinity of 0.9% (w/v), aeyeop, corolla, bellflower leaves, and Jeju joritdae were added in the same amount as in Example 1, and a beverage composition was prepared according to the method of Example 1.

Comparative Example 10

Herbal medicine leachate was prepared by adding 4g of aerobatic leaves, 4g of brown tiger color, 6g of bellflower leaves, and 3g of Jeju joritdae based on 100g of normal mineral water, and stirring at 4℃ at 20rpm for 24 hours.At this time, bamboo salt prepared by firing sea salt/bamboo/oyster bark was used. The salinity was adjusted to 0.9% (w/v), and the water-soluble components calcined from the oyster shell including calcium were sufficiently dissolved in mineral water by stirring at 20 rpm at 4° C. for 1 hour. Next, the supernatant obtained by centrifugation at 10000 rpm for 30 minutes was filtered by sequentially applying a nonwoven fabric filter, a filter paper filter, and a filter having 0.2 μm pores. Thereafter, the crude drug leachate was kept at 65° C. for 30 minutes to pasteurize, and after pasteurization, 1 g of monelin and 0.5 g of taumatin were added based on 100 g of the crude drug leachate, followed by ultraviolet sterilization and enclosed in a sterilization pack.

<Experimental Example 1. Preparation of experimental animals and administration of samples>

Experimental Example 1-1. Breeding of experimental animals and administration of bamboo saline

As experimental animals, male rats of Sprague-Dawley, weighing 170-180 g, were purchased from Daehan Biolink Co., Ltd. (Eumseong, Korea). The breeding environment was maintained at 20±2℃ temperature, 55±1% (RH) humidity, and 12 hour intervals of light and dark cycles, and circulated for 1 week. The feed was given a certain amount of solid feed for experimental animals (PMI Nutrition International) at a certain time every day, and the water was freely ingested using sterile water using a water bottle.

In addition, before the experiment, the bamboo saline or saline solution of Examples 1 to 4 and Comparative Examples 1 to 10 was orally administered 1 ml each day to each experimental animal population (8 animals per group) for 2 weeks.

<Experimental Example 2. Anti-stress effect confirmation>

Experimental Example 2-1. Induction of restraint stress and blood collection

Restraint stress was applied to a group of animals to which each bamboo saline solution or saline solution (Examples 1 to 4 and Comparative Examples 1 to 10) was administered for 2 weeks.

The method of applying restraint stress is an anti-stress composition. Each experimental animal ingested with bamboo saline or saline solution for 2 weeks is placed in a transparent case (40cm×30cm×30cm), and the case is placed in a vibrator for 1 hour every day from 17:00 to 18:00. After placing, it was carried out for 7 days by vibrating (2km/hr). At this time, each bamboo saline solution or saline solution was administered even during the period of applying restraint stress.

10 minutes after completion of the 7-day restraint stress, each experimental animal is anesthetized and sacrificed, and an analysis sample (serum or plasma, etc.) is collected, and cortisol in blood is used as a biomechanical to evaluate the anti-stress effect in the next experiment. ), lactic acid, glucose, and creatine phosphokinase were measured.

Experimental Example 2-2. Comparison of the amount of cortisol in the blood

When stress is triggered, the human body secretes cortisol to lower the metabolic rate, stimulates the appetite center of the cerebrum, and acts to consume food without hunger (Adam TC, Epel ES. Stress, eating and the reward system. Physiology and Behavior. 2007. 91(4):449-458).

Accordingly, Table 1 shows the amount of cortisol in the blood of animals administered with bamboo saline or saline solution after induction of restraint stress.

Experimental conditions conditions Post restraint stress
Blood cortisol content (㎍/㎗) General group (non-stress induction) 12.3 Non-administration (induces restraint stress only) 42.1 Restraint Stress + Example 1 14.4 Restraint Stress + Example 2 15.2 Restraining Stress + Example 3 16.7 Restraint Stress + Example 4 14.5 Restraint Stress + Comparative Example 1 28.3 Restraint stress + comparative example 2 39.0 Constraint Stress + Comparative Example 3 37.6 Constraint Stress + Comparative Example 4 36.7 Constraint Stress + Comparative Example 5 31.6 Constraint Stress + Comparative Example 6 29.2 Constraint Stress + Comparative Example 7 31.0 Restraint Stress + Comparative Example 8 32.3 Constraint Stress + Comparative Example 9 33.2 Restraint Stress + Comparative Example 10 32.3

As shown in Table 1, it can be seen that the blood cortisol is the lowest in the group to which the bamboo saline solution of Examples 1 to 5 was administered.

Experimental Example 2-3 Comparison of the amount of creatine phosphokinase

There is a report that the concentration of creatine phosphokinase in the blood was increased in experimental animals subjected to restraint stress (Korean Patent No. 10-1315319, a food composition for fatigue recovery including red ginseng extract and turmeric extract).

Accordingly, the amount of creatine phosphokinase in the blood of animals administered with bamboo saline or saline solution after induction of restraint stress is shown in Table 2.

Experimental conditions conditions Post restraint stress
Creatine phosphokinase in blood (mU/ml) General group (non-stress induction) 34.3 No administration (induces restraint stress only) 108.2 Restraint Stress + Example 1 43.4 Restraint Stress + Example 2 42.6 Restraining Stress + Example 3 45.3 Restraint Stress + Example 4 43.2 Restraint Stress + Comparative Example 1 61.3 Restraint stress + comparative example 2 72.2 Constraint Stress + Comparative Example 3 61.7 Constraint Stress + Comparative Example 4 73.4 Constraint Stress + Comparative Example 5 72.8 Constraint Stress + Comparative Example 6 81.2 Constraint Stress + Comparative Example 7 74.1 Restraint Stress + Comparative Example 8 69.2 Constraint Stress + Comparative Example 9 79.8 Restraint Stress + Comparative Example 10 75.4

As shown in Table 2, it can be seen that the blood creatine phosphokinase was significantly reduced in the group to which the bamboo saline solution of Examples 1 to 4 was administered.

<Experimental Example 3. Confirmation of anti-fatigue effect through swimming exercise>

Experimental Example 3-1. Swimming exercise test

A swimming exercise load experiment was conducted in each group of bamboo saline or saline solution administered for 2 weeks. In the swimming exercise load test, a weight of 5% body weight was hung on the back of the neck, received in a constant temperature water tank, and the total swimming time immediately after the determination of exhaustion (when swimming so that the nose was submerged for 5 seconds and then subsided) was measured. Recorded. Immediately after exhaustion, a sample (serum or plasma) was collected by sacrificing.

To evaluate the anti-fatigue effect, the duration of exercise and the amount of glucose and lactic acid in the blood were measured.

Experimental Example 3-2. Swimming time comparison

The swimming time of the experimental animals to which each bamboo saline solution or saline solution was administered was measured and shown in Table 3 below.

Experimental conditions conditions Swimming time (minutes) Non-administration group 24.0 Example 1 43.2 Example 2 42.3 Example 3 41.3 Example 4 45.0 Comparative Example 1 31.4 Comparative Example 2 29.7 Comparative Example 3 28.4 Comparative Example 4 26.9 Comparative Example 5 28.8 Comparative Example 6 29.6 Comparative Example 7 31.3 Comparative Example 8 29.5 Comparative Example 9 33.3

As shown in Table 3, it was confirmed that in the group to which the bamboo saline solution of Examples 1 to 5 was administered, physical fatigue was relieved and exercise time was increased compared to other administration groups.

Experimental Example 3-3. Comparison of the amount of lactic acid in the blood

Table 4 shows the results of measuring the concentration of lactic acid in the blood after swimming of the experimental animals administered with bamboo saline or saline solution.

Experimental conditions for swimming Lactic acid (㎍/㎗) Non-administration group 5.6 Example 1 2.6 Example 2 2.3 Example 3 2.2 Example 4 2.1 Comparative Example 1 3.8 Comparative Example 2 3.9 Comparative Example 3 4.6 Comparative Example 4 4.2 Comparative Example 5 3.3 Comparative Example 6 3.7 Comparative Example 7 3.6 Comparative Example 8 4.2 Comparative Example 9 4.5

As shown in Table 4, in the group treated with the bamboo saline solution of Examples 1 to 5, it was found that the increased amount of lactic acid caused by insufficient oxygen supply in the blood was effectively suppressed by relieving physical fatigue compared to the other treatment groups.

<Experimental Example 4. Confirmation of anti-fatigue effect through treadmill exercise>

Experimental Example 4-1. Treadmill exercise test

A treadmill manufactured so that a mouse or rat can run continuously by applying a certain amount of electrical stimulation is set at an inclination angle of 5° and a running speed of 5 m/min, and each experimental animal group is allowed to run for 20 minutes each day for 7 days, and then blood is collected and transferred. Each experiment was carried out in the same way as described above.

Experimental Example 4-2. Comparison of the amount of lactic acid in the blood

By comparing the lactic acid in the blood of the animal groups subjected to the trade mill exercise, the results shown in Table 5 were obtained, and in this experiment, it can also be seen that the amount of lactic acid produced in the bamboo saline administration group of Examples 1 to 4 was reduced.

Experimental conditions conditions Blood lactate after trademill exercise (㎍/㎗) Non-administration group 6.67 Example 1 2.86 Example 2 2.32 Example 3 2.42 Example 4 4.13 Comparative Example 1 4.98 Comparative Example 2 3.96 Comparative Example 3 3.56 Comparative Example 4 3.22 Comparative Example 5 4.03 Comparative Example 6 4.97 Comparative Example 7 4.56 Comparative Example 8 4.42 Comparative Example 9 4.45

Experimental Example 4-3. Comparison of the amount of glucose in the blood

By comparing the glucose in the blood of the animal groups subjected to the trade mill exercise, the results shown in Table 6 were obtained, and in this experiment, it can also be seen that the amount of glucose consumed in the bamboo saline administration group of Examples 1 to 4 was large.

Experimental conditions conditions Blood glucose content after trademill exercise (㎍/㎗) Non-administration group 25.1 Example 1 12.8 Example 2 11.4 Example 3 11.2 Example 4 12.1 Comparative Example 1 19.4 Comparative Example 2 19.2 Comparative Example 3 22.5 Comparative Example 4 22.4 Comparative Example 5 24.5 Comparative Example 6 25.6 Comparative Example 7 18.2 Comparative Example 8 19.2 Comparative Example 9 20.2 Comparative Example 10 19.1

<Experimental Example 5. Dosing test-Fatigue recovery improvement effect confirmation>

Each of 7 men in their 30s was allowed to drink 500 ml of bamboo saline or saline solution of Examples 1 to 4 and Comparative Examples 1 to 10 for 20 days, 1 hour of swimming per day and 1 hour of trade mill exercise every day. The degree of improvement in fatigue after taking is compared with before taking each composition in a 5-point Likert measurement method (5 points: very good, 4 points: excellent, 3 points: normal, 2 points: bad, 1 point: very bad). To show. To this end, the effect of improving the sleep effect, the feeling of helplessness, and the effect of improving the overall feeling of fatigue were confirmed and shown in Table 7.

division Sound sleep effect Fatigue improvement effect Example 1 4.2 4.2 Example 2 4.2 4.3 Example 3 4.5 4.1 Example 4 4.2 4.3 Comparative Example 1 3.1 2.9 Comparative Example 2 3.2 3.0 Comparative Example 3 2.5 2.2 Comparative Example 4 2.7 2.9 Comparative Example 5 2.4 2.9 Comparative Example 6 2.2 2.0 Comparative Example 7 3.1 3.1 Comparative Example 8 2.9 2.8 Comparative Example 9 3.1 3.0 Comparative Example 10 3.1 3.1

As shown in Table 7, it can be seen that in the group treated with bamboo saline water of Examples 1 to 4, there was a sound sleep effect and an effect of improving fatigue compared to the group taking Comparative Examples 1 to 10.

Therefore, it can be seen that the composition of the present invention has excellent stress relief effect, anti-fatigue effect, or endurance enhancing effect, and those who want to improve physical strength through exercise through these functions, youth, adolescent students, examinees, and the elderly , It can be seen that it can be used as a high-quality drink for physical strength recovery for patients in recovery period and patients during rehabilitation exercise. In addition, through the above results, the physiological activity function of the bamboo saline produced by the present invention is improved compared to that of a general purified water extract or a drink using a solvent extract. It is believed that the use of natural protein sweeteners, monelin and taumatin, is used instead of sweeteners such as oligosaccharides and sugars.

Claims (5)

(First step) preparing bamboo salt containing CaO by mixing the sea salt with bamboo and oyster shells in a weight ratio of 1:0.5:0.5 to 1:2:2 and firing at 900 to 1200°C for 6 to 24 hours;
(Second step) The bamboo salt is suspended in bottled water so that its salinity is 0.8 to 1.0% (w/v), stirred at 4 to 10°C for 18 to 24 hours at 10 to 30 rpm, and then insoluble substances are removed to remove calcium (Ca ^{2 +} ) Preparing bamboo salt mineral water with enhanced content;
(Third step) In the bamboo salted water obtained in the second step, add aeop, hyeonho color, bellflower leaves, and jeju joritdae, stir at 4 to 10°C for 18 to 24 hours at 10 to 30 rpm, and then filter to prepare a herbal medicine leaching bamboo salt water. The step of doing;
(Step 4) Pasteurizing by maintaining the herbal medicine leaching bamboo salt mineral water obtained in the third step at 62 to 65°C for 30 to 40 minutes; And,
(Step 5) adding monelin and taumatin to the herbal medicine leaching bamboo salt water after pasteurization, UV sterilization, and encapsulating in a sterilization pack;
A method for producing bamboo salt water for fatigue relief, characterized in that produced, including. delete Bamboo salt water for fatigue relief, characterized in that produced by the method of claim 1. The method of claim 3,
The bamboo salt water is bamboo salt water for fatigue relief, characterized in that it has the effect of inhibiting the secretion of one or more substances selected from cortisol, phosphokinase and lactic acid. The method of claim 3,
The bamboo salt water is bamboo salt water for fatigue relief, characterized in that it has the effect of enhancing the consumption of glucose in the blood.