KR102157970B1 - Cosmetic composition containing lactic acid bacteria fermented extract of germinated sprout for skin barrier reinforcement - Google Patents

Abstract

The present invention relates to a cosmetic composition for strengthening skin barrier containing a fermented product of lactic acid bacteria from a germinated sprout extract and to a cosmetic composition having a skin barrier strengthening function and an anti-pollution function by containing a fermented product in which germinated sprout extracts of broccoli, rape, sunflower, and barley are fermented with lactic acid bacteria, preferably Lactobacillus gasseri strain, more preferably Lactobacillus gasseri (Lactobacillus gasseriSKB1102, accession number: KCCM12342P) strain. The fermented product enhances the expression of Involucrin and Claudin-1, which are related to the skin barrier, and has excellent regeneration efficacy and anti-pollution efficacy of skin cells damaged by UV rays. Therefore, the composition can be usefully used as a functional cosmetic that can keep the skin healthy.

Description

Cosmetic composition containing lactic acid bacteria fermented extract of germinated sprout for skin barrier reinforcement}

The present invention relates to a cosmetic composition for strengthening the skin barrier containing the fermented product of lactic acid bacteria germinated sprout extract. In more detail, the present invention uses broccoli, rapeseed, sunflower, and barley germination sprout extracts, lactic acid bacteria, preferably Lactobacillus gasseri strain, more preferably Lactobacillus gasseri ( Lactobacillus gasseri SKB1102, accession number: KCCM12342P ) It relates to a cosmetic composition that contains the fermented strain and has a skin barrier strengthening function and an anti-pollution function.

Skin is largely divided into three layers: epidermis, dermis, and subcutaneous tissue in order from the outside, and has the function of protecting the internal organs from temperature and humidity changes, ultraviolet rays, and other physical and chemical stimulations of the external environment. In particular, the epidermis plays an important role in preventing water evaporation inside the human body. The epidermis is divided into a stratum corneum layer, a granule layer, a play layer, and a basal layer in order from the outside, and the cells of the stratum corneum act like bricks, and the intercellular lipids between the keratinocytes act like mortar to form the skin barrier. In addition, a high concentration of natural moisturing factor (NMF) exists in the keratinocytes of healthy humans to help retain moisture in the skin.As in these days, artificial temperature control of cooling/heating according to changes in environment or lifestyle changes. , Skin stress caused by various stresses and environmental pollution generated in social life, frequent cleansing according to makeup habits, and natural skin aging due to an increase in age. The need for a composition for strengthening the skin barrier or for moisturizing the skin is increasing because it becomes rough and the skin becomes crumbly and the skin loses its moistness and looks dull.

The skin performs various functions essential for the human body to survive. Barrier functions to maintain homeostasis inside the human body in response to environmental changes, sensory functions to recognize external changes, and body temperature control functions are among the most representative skin functions, all of which function inside the body in response to changes in the external environment. It plays a role in maintaining homeostasis that can maintain. Among the various functions of the skin, in particular, the barrier function of the skin is mainly manifested by the stratum corneum located at the outermost part of the skin. However, according to recent research results, the stratum corneum has been reported to affect not only the simple barrier function but also the function, role, and structure of the inner living cell layer, that is, the epidermal layer or the dermal layer, and its importance is continuously increasing. . As the importance of the stratum corneum is emphasized, research on this is being actively conducted.

The decrease in hyaluronic acid, an extracellular matrix component that plays a major role in the skin's moisture retention ability, means that the moisture in the stratum corneum is reduced, resulting in loss of its original function and becoming rough. In addition, filaggrin is a major protein in the epidermis, and the increase of filaggrin means that it prevents evaporation of skin moisture by forming a normal skin barrier. Therefore, the expression of HAS3, which is responsible for synthesizing hyaluronic acid, and the increase of filaggrin may improve skin barrier function by maintaining and improving the function of the skin epidermis. In addition, external stress, such as a contaminated environment, induces skin irritation and eventually leads to skin aging.

Among these external stimuli, in particular, fine dust, yellow dust, and smog mixed with harmful substances such as heavy metals, which have recently occurred due to industrialization, are recognized as major causes of skin aging and skin problems. Heavy metal refers to metals with a specific gravity of 4 or more, such as mercury, cadmium, lead, and copper. Generally, when absorbed into a living body, it combines with the in vivo substance to form an organic complex that is not easily decomposed, so it is not quickly discharged out of the body. , It accumulates in organs such as kidneys and bones. When exposed to heavy metals for a long period of time, symptoms such as paralysis, abnormal movement, ataxia, pigmentation and keratinization of the skin, atrophy defects of toenails or hoofs and hair, reproductive dysfunction, birth defects, decreased growth and decreased immune function. Environmentally hazardous substances are recognized as one of the most harmful environmental problems to health, and are considered to be highly related to skin diseases.

In the skin, harmful heavy metal fine dusts cause chemical reactions in the air, thereby additionally generating harmful substances such as nitrogen oxides (NO) and sulfur oxides (SO), increasing cytokines, which are inflammatory substances in the skin, and reducing atopy and skin problems. Can cause. On the other hand, since the size of the heavy metal particles is 2 μm or less, it is much smaller than that of general dust (average size is 20 μm or more). Since the fine dust containing such heavy metals is small, it can easily penetrate deep into skin pores. If the penetrating fine dust is not removed cleanly, it often causes inflammation and troubles on the skin. * Fine dust or fine particulate matter: Pollution material (PM) with a diameter of 2.5 to 10 µm or pollutants less than 2.5 µm.

Currently, the method of removing heavy metals from the body is a method of discharging heavy metals out of the body by injecting complex compound-forming substances such as EDTA (ethylene diamine tetraacetate) and BAL (British Anti-Lewisite) to bind with the heavy metals accumulated in the body, or vitamin B1, Taking vitamin C and vitamin E to prevent the absorption of heavy metals in the body and to discharge them. However, there is no known method for effectively and easily protecting skin from heavy metals. Clinically, when environmentally hazardous substances directly contact the skin, it causes allergic reactions and irritation, which is prone to contact dermatitis, and the dry, strong yellow dust breeze takes away moisture from the skin, resulting in dry skin, dead skin cells, itchiness, acne and atopy. It is known to cause the back, but the treatment or countermeasure for this is not clear.

As a result of research efforts on new natural substances that can protect skin from environmentally harmful factors, the applicant of the present invention suppresses the expression of AhR (Aryl-hydrocarbon receptor) gene and prevents skin damage due to heavy metals. The present invention was completed by confirming that it not only improves but also strengthens the skin barrier.

Republic of Korea Patent Registration No. 10-1953072 (Name of invention: novel Lactobacillus gasseri SKB1102 strain or cosmetic composition containing anti-pollution function, Applicant: SK Bioland Co., Ltd., registration date: February 21, 2019) Republic of Korea Patent Publication No. 10-2019-0048152 (Name of invention: cosmetic composition containing fermented milk cream of lactic acid bacteria as an active ingredient, Applicant: Konkuk University Industry-Academic Cooperation Foundation, Publication Date: May 9, 2019) Korean Patent Laid-Open Patent No. 10-2019-0079836 (Name of the invention: Method for producing fermented sprout barley with improved saponarin, isobitexin, and luteolin content using a new Lactobacillus fermentum strain, Applicant: Freshbell Co., Ltd. , Release date: July 08, 2019) Republic of Korea Patent Registration No. 10-1975031 (Name of invention: novel Bifidobacterium longum ATG-F5 strain or cosmetic composition containing the same, Applicant: A2Gen Co., Ltd., registration date: April 26, 2019)

An object of the present invention is to provide a cosmetic composition for strengthening the skin barrier containing the fermented product of lactic acid bacteria germinated sprout extract. In more detail, the object of the present invention is to extract germinated sprouts of broccoli, rapeseed, sunflower, and barley as lactic acid bacteria, preferably Lactobacillus gasseri strains, more preferably Lactobacillus gasseri SKB1102, accession number : KCCM12342P) to provide a cosmetic composition that contains fermented strain and has skin barrier reinforcement and anti-pollution function.

The present invention relates to a cosmetic composition for strengthening the skin barrier, characterized in that it contains the fermented product of lactic acid bacteria germinated sprout extract.

The germinated sprout extract may be solvent-extracted from germinated sprouts of broccoli, rapeseed, sunflower, and barley.

The lactobacillus is characterized in that it is a Lactobacillus gasseri strain, more preferably Lactobacillus gasseri SKB1102 ( Lactobacillus gasseri SKB1102, accession number: KCCM12342P) It is characterized in that it is a strain.

The fermented product of lactic acid bacteria of germinated sprout extract has an anti-pollution function, and in particular, it is anti-pollution against at least one environmental harmful factor selected from the group consisting of fine dust, yellow dust, vehicle exhaust gas, dioxin, benzopyrene and heavy metals contained in the air. It is characterized by having a pollution function.

The fermentation product is characterized by inhibiting the expression of AhR (Aryl-hydrocarbon receptor) and CYP1A1 (Cytochrome P450, Family 1, member A1) or (CYP1A1 (Cytochrome P450, Family 1, member A1) genes).

The fermentation product is characterized by having a function of regenerating skin cells against UV damage, and has an effect of increasing the production of PGC-1α, which is reduced due to UV damage. (PGC-1α: Peroxisome proliferator-activated receptor-gamma coactivator 1α)

The skin cells may be selected from one or more of human fibroblasts and human keratinocytes.

The present invention also provides a cosmetic composition for strengthening the skin barrier comprising a strain of Lactobacillus gasseri ( Lactobacillus gasseri SKB1102, accession number: KCCM12342P). The strain or its culture medium is characterized by having a function of regenerating skin cells against UV damage.

The strain or a culture solution thereof, the bacterial body of the strain; Lysate of the cells; Culture of the strain; A culture solution from which cells are removed from the culture of the strain; Cell extract of the strain; An extract of the culture of the strain; Or an extract of the culture medium from which the cells are removed from the culture of the strain; It may be selected from among.

Hereinafter, the present invention will be described in detail.

The lactic acid bacteria fermented product of the germinated sprout extract of the present invention can be cultured by inoculating the Lactobacillus gasseri strain SKB1102 after preparing the germinated sprout extract, 30-40° C., pH 4.0-6.5, 24 hours-2 days Do. At this time, the strain is preferably inoculated so as to be 1 to 10% by volume of the extract in a culture solution of 10 ⁷ to 10 ⁹ CFU/ml. The culture medium may be obtained by culturing the Lactobacillus gaseri SKB1102 strain in MRS Broth.

In addition, the extract or fermented product thereof may be used by filtration or concentration, and in this case, it is necessary after filtering the extract or fermented product with a filter filter having a mesh size of 0.1 to 10 µm, preferably 250 mesh to 1.0 µm. It can be concentrated according to. The concentration process may be performed using a concentrator used in the manufacture of conventional raw materials for pharmaceuticals, foods, or cosmetics, and it is preferable to concentrate using a vacuum concentration method.

The germinated sprout extract or the fermented product obtained by fermenting the sprout may be diluted with an appropriate solvent as necessary, and preferably 1,3-butylene glycol (1,3-Butylene Glycol) may be used.

The fermented product may be sterilized or sterilized after fermentation, and preferably, a method of applying heat at 90 to 110° C. for 20 to 30 minutes may be used.

The sprout solvent extract for germination of broccoli, rapeseed, sunflower and barley contained in the cosmetic composition of the present invention germinates broccoli, rapeseed, sunflower and barley, which are samples for extraction, and when sprouts sprout, all the outposts from the root to the sprout are mixed as it is. It may be a solvent extraction.

At this time, water, a lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, chloroform, ethyl acetate, or a mixture thereof may be used as an extraction solvent. The lower alcohol having 1 to 4 carbon atoms may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol and isobutanol.

The extraction solvent used in the preparation of the extract is a volume of 1 to 40 times (1 to 40 ℓ per 1 kg) or weight (1 to 40 kg per 1 kg) of 1 to 40 times the weight of germination sprouts (germinated sprouts mixture) of broccoli, rapeseed, sunflower and barley Can be used, preferably 5 to 40 times the volume or weight may be used. Extraction conditions for the extract may be 30 minutes to 48 hours at 20 ~ 100 ℃. The process can be repeated up to 1 to 4 times.

In addition, as a conventional method in the art, after dissolving the extract in water, fractions were additionally fractionated using at least one solvent selected from the group consisting of n-hexane, methylene chloride, acetone, chloroform, ethyl acetate, and n-butanol. It can be manufactured with.

The manufacturing temperature of the extract may be 20 to 100 ℃, but is not limited thereto. The extraction time is not particularly limited, but it is preferable to extract within 10 minutes to 2 days, and a conventional extraction device, an ultrasonic grinding extractor, or a fractionator may be used as an extraction device.

When preparing the extract, when filtering the extract, the filtration is a process of removing suspended solid particles from the extract, and the particles can be filtered using cotton, nylon, paper, etc., or ultrafiltration, freezing filtration, centrifugation, etc. can be used. However, it is not limited thereto.

The extraction method during the preparation of the extract is not particularly limited, and according to the type and extraction method of the extract, for example, cold needle extraction, ultrasonic extraction, reflux cooling extraction, room temperature extraction, hot water extraction, and the like may be used.

The prepared extract includes, but is not limited to, hot air drying, vacuum drying, freeze drying, vacuum drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying, and the like. In some cases, a process of pulverizing the final dried extract may be added.

In addition, the extract may be used after purification using column chromatography. The chromatography was performed on silica gel column chromatography, LH-20 column chromatography, ion exchange resin chromatography, and medium pressure liquid chromatography. chromatography), thin layer chromatography (TLC), silica gel vacuum liquid chromatography, and high performance liquid chromatography.

The strain of the present invention or a cosmetic composition comprising the same is one having anti-pollution efficacy against at least one environmentally harmful factor selected from the group consisting of fine dust, yellow dust, vehicle exhaust gas, dioxin, benzopyrene and heavy metals contained in the air. In this case, the heavy metal may be a metal having a specific gravity of 4 or more and may have a specific gravity of at most 23, such as mercury (13.5), cadmium (8.7), lead (11.3), and copper (8.9).

The present invention provides a cosmetic composition containing the lactic acid bacteria fermented product of the sprout sprout extract, the composition may contain 0.1 to 20% by weight of the lactic acid bacteria fermented product of the germination sprout extract.

The formulation of the cosmetic composition is not particularly limited, but preferably, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, It can be selected from the group consisting of essence, nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser.

The cosmetic composition of the present invention may further include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingo lipids, and seaweed extract.

The water-soluble vitamin may be any one that can be blended in cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, etc. And their salts (thiamine hydrochloride, sodium ascorbate, etc.) and derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-magnesium salt, etc.) are also included in the water-soluble vitamins that can be used in the present invention. Included. Water-soluble vitamins can be obtained by conventional methods such as a microbial transformation method, a purification method from a culture of microorganisms, an enzyme method or a chemical synthesis method.

As the oil-soluble vitamin, any one may be used as long as it can be blended in cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), etc. , Their derivatives (ascorbine palmitate, ascorbine stearate, ascorbine dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinate, vitamin E, DL-pantotenyl alcohol, D-pantotenyl alcohol, pantotenyl ethyl Ether, etc.) are also included in the oil-soluble vitamin used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as a microbial transformation method, a purification method from a microorganism culture, an enzyme or a chemical synthesis method.

The polymer peptide may be any one as long as it can be blended into a cosmetic, but preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like are exemplified. The polymeric peptide can be purified and obtained by a conventional method such as a purification method from a culture medium of a microorganism, an enzyme method, or a chemical synthesis method, or it can be used after being purified from natural products such as dermis of pigs and cattle, silk fibers of silkworms.

The polymer polysaccharide may be any one as long as it can be blended in the cosmetic composition, but preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfuric acid or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or a salt thereof can be used after being purified from mammals or fish.

The sphingo lipid may be any one as long as it can be blended into the cosmetic composition, and ceramide, phytosphingosine, sphingoglycolipid, and the like are preferably mentioned. Sphingo lipids are usually purified from mammals, fish, shellfish, yeast, plants, etc. by a conventional method, or can be obtained by chemical synthesis.

The seaweed extract may be anything as long as it can be blended into the cosmetic composition, but preferably brown algae extract, red algae extract, green algae extract, etc. are exemplified, and carrageenan, arginic acid, sodium arginate purified from these seaweed extracts And potassium arginate are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purifying from seaweed by a conventional method.

In the cosmetic composition of the present invention, other ingredients to be blended in conventional cosmetics may also be blended.

Other ingredients that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, Blood circulation accelerators, cold sensation agents, restrictors, and purified water.

Examples of oil and fat components include ester oils and fats, hydrocarbon oils, silicone oils, fluorine oils, animal oils and vegetable oils.

Examples of ester oils include glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, stearic acid. Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adipine Diisopropyl acid, isoalkyl neopentanoate, tri(capryl, capric acid) glyceryl, tri2-ethylhexanoate trimethylolpropane, triisostearate trimethylolpropane, tetra2-ethylhexanoate pentaelisitol , Cetyl caprylate, decyl laurate, hexyl laurate, decyl myristic acid, myristic myristic acid, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinooleate, isostea laurate Reel, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, 2-ethylhexanoate cetoste Aryl, 2-ethylhexanoate stearyl, isostearate hexyl, dioctanoate ethylene glycol, dioleate ethylene glycol, dicaprylic acid propylene glycol, dicaprylic acid propylene glycol, dicaprylic acid propylene glycol, dica Neopentyl glycol prinate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate , Octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerololeic acid ester, polyglycerin isostearate, Triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, di malic acid Isostearyl, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, Cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, pitsteryl isostearate, pitsteryl oleate, 12-stealoyl And esters such as isocetyl hydroxystearate, stearyl 12-stealoylhydroxystearate, and isostearyl 12-stealoylhydroxystearate.

Examples of hydrocarbon-based fats and oils include hydrocarbon-based fats such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybuden, microcrystalline wax, and petrolatum.

Silicone-based fats and oils include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane/methylcetyloxysiloxane copolymer, dimethylsiloxane/methylsteaoxysiloxane copolymer, alkyl And modified silicone oil and amino-modified silicone oil.

As fluorine-based fats and oils, perfluoropolyether, etc. are mentioned.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed Oil, Palm Oil, Cucuine Nut Oil, Wheat Germ Oil, Rice Germ Oil, Shea Butter, Walnut Colostrum Oil, Marker Demi Anut Oil, Meadow Home Oil, Egg Yolk Oil, Tallow Oil, Horse Oil, Mink Oil, Orange Rape Oil, Jojoba Oil , Animal or plant fats such as canderry wax, carnauba wax, liquid lanolin, and hydrogenated castor oil.

Examples of the moisturizing agent include a water-soluble low-molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular weight moisturizers include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactate, and the like.

As a fat-soluble low molecular weight moisturizer, cholesterol, cholesterol ester, etc. are mentioned.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid salt, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, and the like. I can.

Examples of the oil-soluble polymer include polyvinylpyrrolidone/eicosene copolymer, polyvinylpyrrolidone/hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollient agent include long-chain acyl glutamate cholesteryl ester, hydroxystearate cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POE·POP (polyoxyethylene·polyoxypropylene) copolymer, POE·POP alkyl ether, polyether-modified silicone, lauric acid Alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

As anionic surfactants, fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphate, alkyl amide Phosphate, alkyloylalkyltaurine salt, N-acylamino acid salt, POE alkylether carboxylate, alkyl sulfosuccinate, sodium alkylsulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate, and the like. have.

Cationic surfactants include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyldimethylbenzyl ammonium chloride, behenyl trimethyl ammonium bromide, and chloride. Benzalkonium, diethylaminoethyl amide stearate, dimethylaminopropyl amide stearate, lanolin derivative quaternary ammonium salt, and the like.

Examples of amphoteric surfactants include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amideamine type, etc. Amphoteric surfactants, and the like.

Examples of organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide. , Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine, chromium oxide, chromium hydroxide, calamine, and complexes thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene-styrene copolymer, Organic pigments, such as silk powder, cellulose, CI pigment yellow, and CI pigment orange, and complex pigments of these inorganic pigments and organic pigments, etc. are mentioned.

Examples of the organic powder include metal soaps such as calcium stearate; Alkyl phosphate metal salts, such as sodium zinc cetylate, a zinc laurylate, and calcium laurylate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroyl glycine calcium; Amide sulfonic acid polyvalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N, such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoyl lizine, N-alpha-paritoylolnitine, N-alpha-lauroylarginine, N-alpha-hardened tallow fatty acid acylarginine, etc. -Acyl basic amino acid; N-acyl polypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene/styrene copolymer, ethylene tetrafluoride, and the like.

UV absorbers include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoate, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicate, butyl phenyl salicylate, homomentyl salicylate, benzyl cinnamate , Paramethoxycinnamic acid-2-ethoxyethyl, paramethoxycinnamic acid octyl, diparamethoxycinnamic acid mono-2-ethylhexane glyceryl, paramethoxycinnamic acid isopropyl, diisopropyl·diisopropyl cinnamic acid ester mixture, right Cannic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)-1 ,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, etc. are mentioned.

As a disinfectant, hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinphylithione, benzalkonium chloride, photosensitization Sono No. 301, mononitroguarous sodium, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and elisorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, pmalic acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogen phosphate, and the like.

Examples of alcohol include higher alcohols such as cetyl alcohol.

In addition, the blending ingredients that may be added are not limited thereto, and any of the above ingredients can be blended within a range that does not impair the object and effect of the present invention, but preferably 0.01 to 5% by weight, more preferably, based on the total weight. May be blended in 0.01 to 3% by weight.

The cosmetic composition of the present invention may take the shape of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetics in addition to the extract as an active ingredient. For example, conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances, and It includes a carrier.

When the cosmetic composition formulation of the present invention is a paste, cream, or gel, animal fibers, plant fibers, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide, etc. are used as carrier components. Can be used.

When the cosmetic composition formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbons , Propane/butane or a propellant such as dimethyl ether.

When the cosmetic composition formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the cosmetic composition formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

When the cosmetic composition formulation of the present invention is a surfactant containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Fatty acid amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linoline derivatives, or ethoxylated glycerol fatty acid esters may be used.

The present invention relates to a cosmetic composition for strengthening the skin barrier containing the fermented product of lactic acid bacteria of the germinated sprout extract, wherein the germinated sprout extracts of broccoli, rapeseed, sunflower and barley are lactic acid bacteria, preferably Lactobacillus gasseri strain, More preferably, it relates to a cosmetic composition having a skin barrier strengthening function and an anti-pollution function, containing fermented strains of Lactobacillus gasseri SKB1102, accession number: KCCM12342P.

The fermentation product enhances the expression of Involucrin and Claudin-1, which are related to the skin barrier, and has excellent regeneration efficacy and anti-pollution efficacy of skin cells damaged by UV rays. It can be usefully used as a functional cosmetic that can maintain healthy.

1 shows the results of confirming the effect of increasing the expression of Claudin-1, a tight junction protein, to the fermentation product of Example 2 of the present invention.
FIG. 2 shows the results of confirming the effect of increasing the expression of Involucrin, a strengthen skin barrier, for the fermented product of Example 2 of the present invention.
3 is a graph showing numerical results of JC staining to confirm the efficacy of recovering damage to skin cells due to UV for the fermented product of Example 2 of the present invention.
4 shows the results of confirming the function of recovering damage to skin cells (photoprotective efficacy) due to UV in the fermentation product of Example 2 of the present invention through the expression of PGC-1α, a factor related to mitochondrial biogenesis.
5 and 6 show the results of confirming the damage recovery function (Anti-pollution efficacy) of skin cells due to benzopyrene for the fermented product of Example 2 of the present invention through expression of AhR and CYP1A1.

Hereinafter, a preferred embodiment of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided to sufficiently convey the spirit of the present invention to those skilled in the art so that the contents introduced herein are thorough and complete.

<Example 1. Preparation of sprout extract>

Broccoli sprouts, rapeseed sprouts, sunflower sprouts, and barley sprouts were extracted with 50% ethanol at the same ratio to obtain sprout extract.

Next, the sprout extract was filtered with a filter having a mesh size of 0.1 to 10 μm, preferably 1.0 μm. After the filtered filtrate was concentrated under reduced pressure, 1,3-Butyelene Glycol was added to prepare a sprout extract. The concentration of the sprout extract after that was described by diluting the concentrate from which the solvent was removed in 1,3-Butylene Glycol or converting it to the final value when included in the medium.

<Example 2. Preparation of lactic acid bacteria fermented product of sprout extract>

Broccoli sprouts, rapeseed sprouts, sunflower sprouts, and barley sprouts were extracted with 50% ethanol at the same weight ratio (1:1:1:1) to obtain a sprout extract.

Next, the sprout extract was filtered with a filter having a mesh size of 0.1 to 10 μm, preferably 1.0 μm. After removing ethanol by concentrating the filtered filtrate under reduced pressure, lactic acid bacteria starter (MRS medium culture medium) with a number of lactic acid bacteria of 10 ⁷ to 10 ⁹ CFU/ml was inoculated at a volume of 5%, followed by anaerobic fermentation at 37°C for 24 to 48 hours. Prepare the fermented product.

After heating the lactic acid bacteria fermented product at 110° C. for 15 minutes, it was filtered and 1,3-Butylene Glycol was added thereto, which was used in the next experiment. The concentration of the fermented product of lactic acid bacteria after that was described in terms of the final value when the concentrate from which the solvent was removed was diluted in 1,3-Butylene Glycol or included in the medium.

<Experimental Example 1. Strengthen skin barrier (Tight junction protein-Claudin-1)>

In this experimental example, Claudin-1, one of the Tight Junction (TJ) protein-related genes constituting the skin barrier to measure the skin barrier strengthening effect of Example 2 compared to Example 1 (CLDN-1) expression experiments were performed. Inoculate normal human keratinocytes (gibco) in cell culture dish with 5.0 Х10 ⁵ cells, culture them for 1 day (37℃, CO ₂ 5%), and replace them with Serum-free medium (Epilife, gibco). Then, the samples are treated by concentration and incubated in a 37°C, CO ₂ 5% incubator for 24 hours. After culture, the cells are collected with TRIzol and RNA is isolated according to the manufacturer's method. After quantifying the isolated RNA, cDNA is synthesized with 1 μg of RNA, and real-time PCR is performed. As a positive control, Lactoferrin, which is known to be effective in strengthening the barrier as one of the ingredients of colostrum, was used. The expression level of Claudin-1 was a relative value to β-actin, one of the house keeping genes, and the average value (%) of the data tested three times was described. The primer used for PCR was synthesized and used by Cosmo Genetech (Korea). And the results are shown in Table 1 and Figure 1.

Sample Relative Claudin-1 mRNA expression (INV/β-actin) (%) Stdev. P-value Control 100.00 0.00 1.0000 Example 1
(%, v/v) 0.1 101.35 5.83 0.1815 0.5 105.27 2.97 0.0180 1.0 110.35 9.59 0.0049 Example 2
(%, v/v) 0.1 113.59 7.37 0.0012 0.5 121.78 0.72 0.0000 1.0 139.29 3.93 0.0021 Lactoferrin 1 μM 150.62 4.73 0.0000

As a result of confirming the expression of Claudin-1, one of the tight junction proteins that plays a decisive role in skin barrier function, Example 2 was found to significantly increase the expression of Claudin-1 in a concentration-dependent manner compared to Example 1. Therefore, compared with Example 1, fermented Example 2 was confirmed to be a cosmetic composition having excellent barrier strengthening effect by showing the effect of inducing the expression of components constituting the skin barrier more excellently.

<Experimental Example 2. Strengthen skin barrier confirmation (Barrier components-Involucrin)>

Next, in order to measure the effect of strengthening the skin barrier of Example 2 compared to Example 1, expression of involucrin (INV), which decisively contributes to the barrier function, was confirmed. Inoculate normal human keratinocytes (gibco) in cell culture dish with 5.0 Х10 ⁵ cells, culture them for 1 day (37℃, CO ₂ 5%), and replace them with Serum-free medium (Epilife, gibco). Then, the samples are treated by concentration and incubated in a 37°C, CO ₂ 5% incubator for 24 hours. After culture, the cells are collected with TRIzol and RNA is isolated according to the manufacturer's method. After quantifying the isolated RNA, cDNA is synthesized with 1 μg of RNA, and real-time PCR is performed. Lactoferrin, which is known to have barrier strengthening effect, was used as a positive control. The expression level of Involucrin was a relative value to β-actin, one of the house keeping genes, and the average value (%) of the data tested three times was described. The primer used for PCR was synthesized and used by Cosmo Genetech (Korea). And the results are shown in Table 2 and Figure 2.

Sample Relative Involucrin mRNA expression (INV/β-actin) (%) Stdev. p-value Control 100.00 0.00 1.0000 Example 1
(%, v/v) 0.1 105.67 4.32 0.0207 0.5 110.35 3.87 0.0015 1.0 114.03 2.48 0.0002 Example 2
(%, v/v) 0.1 120.19 4.96 0.0003 0.5 129.84 2.92 0.0006 1.0 135.35 3.03 0.0058 Lactoferrin 1 μM 122.03 5.09 0.0017

Referring to Table 2 and FIG. 2, for Involucrin, which is one of the constituents serving as a physical barrier, Example 1 showed a very fine increase in expression, but Example 2 noticeably increased the expression amount to a statistically significant level. Appeared to be. Therefore, it is confirmed that Example 2, which is a fermented composition, is a cosmetic composition superior in skin barrier strengthening effect compared to Example 1.

<Experimental Example 3.Skin revitalizing (Cell functional activation; Mitochondrial membrane potential, JC-1 staining>

To verify the change of mitochondrial cell membrane potential, human skin fibroblasts are dispensed into a cell culture plate, and IMDM (Iscove Modified Dulbecco Media, Gibco) containing 10% bovine serum is used at 37°C, 5% CO ₂ incubator. Stabilize in 24 hours. After washing with HBSS (Hank's Balanced Salt solution) buffer, fresh HBSS was added, and UVB 50 mJ/cm ² was irradiated. After replacing the medium with a serum-free medium, the samples of Examples 1 and 2 were treated and cultured in a 37°C, 5% CO ₂ incubator for 20 hours. After removal of the medium, 1.5 ㎍/㎖ of JC-1 solution was treated and reacted for 1 hour without light in a 37°C, 5% CO ₂ incubator, and the medium was removed and observed with a fluorescence microscope. The results are shown in Table 3 and FIG. 3.

Sample Mitochondrial membrane potential
Average(%) Stdev. p-value Control 100.00 4.38 0.0088 UVB 50 mJ/cm ² 81.94 4.87 1.0000 UVB + 0.1 (v/v)% of the extract of Example 1 84.66 1.91 0.4184 UVB + 0.5 (v/v)% of the extract of Example 1 87.27 0.68 0.2401 UVB + Example 1 extract 1.0 (v/v)% 93.02 3.57 0.0337 UVB + Example 2 fermentation 0.1 (v/v)% 90.79 0.86 0.0362 UVB + 0.5 (v/v)% of Example 2 ferment 96.59 2.84 0.0108 UVB + 1.0 (v/v)% of fermented product of Example 2 101.60 0.65 0.0023 UVB + Adenosine 7.0 ㎍/㎖ 91.77 1.92 0.0313

As a result, it was found that the reduced cell damage was recovered due to UVB irradiation, and it was confirmed that there is a concentration-dependent efficacy in Example 2.

<Experimental Example 4. Skin revitalizing confirmation (Mitochondrial biogenesis)>

Human normal fibroblasts are inoculated into cell culture dishes and stabilized in an IMDM containing 10% bovine serum in a 37°C, 5% CO ₂ incubator for 24 hours. Washed with HBSS, irradiated with UVB 50 mJ/cm ² , replaced with serum-free medium, treated by concentration, and incubated in a 37°C, 5% CO ₂ incubator for 24 hours. Cells were collected with QIAzol™ (Qiagen, USA) and proceeded according to the manufacturer's method. After dissolving an appropriate amount of DEPC-treated purified water in an RNA pellet, it is quantified using a Qubit™ fluorometer (Invitrogen, USA) and a Qubit ^® RNA BR Assay Kit (Invitrogen, USA), and cDNA is synthesized for realtime PCR. For cDNA synthesis, a cDNA synthesis kit (qPCRBIO cDNA Synthesis kit, PCRBIOSYSTEMS, UK) was used, and an experiment was performed by the method of Kit. Realtime PCR used Realtime PCR Kit (2x qPCRBIO SyGreen Blue mix Lo-ROX, PCRBIOSYSTEMS, UK), and after performing the experiment by the method of the Kit, quantitative analysis of gene amplification products using Applied Biosystems 7500 FAST Real-Time PCR System do. Each expression level result was a relative value for GAPDH, one of the house keeping genes, and the average value (%) of the data tested three times was described. PGC-1α and GAPDH primers used in PCR were synthesized and used by Cosmo Genetech (Korea). The results are shown in Table 4 and FIG. 4.

Sample PGC-1α mRNA expression
Average(%) Stdev. p-value Control 100.00 1.15 0.0000 UVB 50 mJ/cm ² 72.23 1.45 1.0000 UVB + 0.1 (v/v)% of the extract of Example 1 71.93 3.92 0.9071 UVB + 0.5 (v/v)% of the extract of Example 1 77.73 2.47 0.0292 UVB + Example 1 extract 1.0 (v/v)% 83.63 3.67 0.0142 UVB + Example 2 fermentation 0.1 (v/v)% 83.18 5.30 0.0260 UVB + 0.5 (v/v)% of Example 2 ferment 88.31 1.24 0.0001 UVB + Example 2 fermentation 1.0 (v/v)% 102.32 4.12 0.0003 UVB + EGCG 2.5 ㎍ / ㎖ 84.55 4.13 0.0082

As a result of checking Table 4, it was confirmed that the fermented product of Example 2 had excellent efficacy in increasing the expression of PGC-1α from 0.1 (w/v)%, which is a lower concentration than the extract of Example 1. As a result, Example 2 can be said to be a cosmetic composition that helps skin vitality by making young mitochondria and inducing energy production by increasing the expression of PGC-1α, which decreases with age compared to Example 1.

<Experimental Example 5. Anti-pollution effect confirmation>

For the compositions of Examples 1 and 2, the effect of inhibiting expression of genes related to environmental hazards was measured. For this purpose, human keratinocytes (HaCaT) were inoculated into a cell culture dish at a density of 1×10 ⁶ cells in DMEM added with 10(w/v)% FBS, and 5(w/v)% CO ₂ , 37℃ Incubated for one day in an incubator of. Then, it was replaced with serum free DMEM medium, and benzopyrene (1 ㎍/㎖), which is known as a carcinogen among pollutants constituents, was pre-treated to the treatment group, and cultured in a 37°C, CO ₂ 5% incubator. In addition, the compositions of Examples 1 and 2 were treated by concentration and incubated in a 37°C, CO ₂ 5% incubator for 6 hours. After culture, the cells are collected with TRIzol and RNA is isolated according to the manufacturer's method. After quantifying the isolated RNA, cDNA is synthesized with 1 μg of RNA, and real-time PCR is performed. Each expression level result was a relative value for GAPDH, one of the house keeping genes, and the average value (%) of the data tested three times was described. The primer used for PCR was synthesized and used by Cosmo Genetech (Korea).

The results of real time PCR expression levels of Ahr and CYP1A1 are shown in Tables 5 and 5, and in Tables 6 and 6.

Sample Relative AhR mRNA expression (AhR/GAPDH) (%) Stdev. p-value Control 67.22 3.76 0.0001 Benzopyrene 1 ㎍/㎖ 100.00 0.00 1.0000 Benzopyrene +
Example 1
(%, v/v) 0.1 86.76 2.22 0.0000 0.5 74.87 2.82 0.0000 1.0 76.53 2.04 0.0000 Benzopyrene+
Example 2
(%, v/v) 0.1 59.31 3.96 0.0001 0.5 46.63 2.71 0.0000 1.0 28.31 2.81 0.0000 Benzopyrene + Resveratrol 20 μM 63.96 3.69 0.0001

Sample Relative CYP1A1 mRNA expression (AhR/GAPDH) (%) Stdev. p-value Control 18.65 0.13 0.0001 Benzopyrene 1 ㎍/㎖ 100.00 0.00 1.0000 Benzopyrene+
Example 1
(%, v/v) 0.1 101.02 1.84 0.0007 0.5 92.62 3.16 0.0025 1.0 87.64 1.62 0.0000 Benzopyrene +
Example 2
(%, v/v) 0.1 99.53 1.39 0.5882 0.5 79.57 1.51 0.0000 1.0 69.18 2.24 0.0000 Benzopyrene + Resveratrol 20 μM 74.22 1.61 0.0000

The expression of genes related to environmental carcinogens and environmental hormone metabolism induced by benzopyrene (hazardous air pollutant), an environmentally harmful factor in cells, was confirmed through real time PCR. We confirmed the expression of Cytochrome P450 1A1 (CYP1A1), which plays an important role in metabolizing carcinogens into carcinogens by the Aryl hydrocarbon receptor (AhR) signaling pathway induced by benzopyrene.

As a result, Example 2 significantly decreased the expression of AhR and CYP1A1 in a concentration dependent manner at all concentrations. In particular, it was proved that the fermented product of Example 2 is a cosmetic composition having very excellent anti-pollution efficacy because it exhibited superior inhibitory efficacy than the Resveratrol treatment group.

<Cosmetic formulation example 1. Preparation of flexible lotion>

As shown in the composition of Table 7 below, a flexible lotion (skin, 100 g) containing the lactic acid bacteria fermented product of the sprout sprout extract prepared in Example 2 was prepared according to a conventional method.

Raw material Content (g) Fermented product of lactic acid bacteria from germinated sprout extract 1.0 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Polyoxyethylene (60) hardened castor oil 1.0 ethanol 10.0 Triethanolamine 0.1 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

<Cosmetic formulation example 2. Preparation of nutrient lotion>

A nutrient lotion (lotion, 100g) containing the lactic acid bacteria fermented product of the sprout sprout extract prepared in Example 2 as shown in Table 8 was prepared according to a conventional method.

Raw material Content (g) Fermented product of lactic acid bacteria from germinated sprout extract 1.0 Sitosterol 1.7 Polyglyceryl 2-oleate 1.5 Ceteares 1.2 cholesterol 1.5 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Sunflower Oil 10.0 Carboxyvinyl polymer 0.2 Xanthan gum 0.3 antiseptic a very small amount Spices a very small amount Purified water Balance

<Cosmetic formulation example 3. Preparation of nutritional cream>

As shown in the composition of Table 9 below, a nutritional cream (100g) containing the fermented product of lactic acid bacteria of the sprout sprout extract prepared in Example 2 was prepared according to a conventional method.

Raw material Content (g) Fermented product of lactic acid bacteria from germinated sprout extract 1.0 Sitosterol 4.0 Polyglyceryl 2-oleate 3.0 Ceramide 0.7 Ceteares-4 2.0 cholesterol 3.0 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Sunflower Oil 22.0 Carboxyvinyl polymer 0.5 Triethanolamine 0.5 antiseptic a very small amount Spices a very small amount Purified water Balance

<Cosmetic Formulation Example 4. Preparation of Essence>

As shown in the composition of Table 10 below, an essence (100 g) containing a fermented product of lactic acid bacteria of the sprout sprout extract prepared in Example 2 was prepared according to a conventional method.

Raw material Content (g) Fermented product of lactic acid bacteria from germinated sprout extract 1.0 Sitosterol 1.7 Polyglyceryl 2-oleate 1.5 Ceteares-4 2.0 cholesterol 3.0 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Sunflower Oil 22.0 Carboxyvinyl polymer 0.5 Triethanolamine 0.5 antiseptic a very small amount Spices a very small amount Purified water Balance

<Cosmetic formulation example 5. Preparation of foundation>

As shown in the composition of Table 11 below, a foundation (100g) containing the lactic acid bacteria fermented product of the sprout sprout extract prepared in Example 2 was prepared according to a conventional method.

Raw material Content (g) Fermented product of lactic acid bacteria from germinated sprout extract 1.0 Beeswax 2.0 Cyclomethicone 2.0 Floating paraffin 5.0 Squalane 5.0 Stearic acid 2.0 Lipophilic glycerin monostearate 3.0 Caprylic/Capric Triglyceride 4.0 glycerin 4.0 Propylene glycol 3.0 Butylene glycol 3.0 Triethanolamine 1.0 Aluminum Magnesium Silicate 0.5 Pigment 12.0 antiseptic a very small amount Spices a very small amount Purified water Balance

<Cosmetic formulation example 6. Preparation of hair shampoo>

As shown in the composition of Table 12 below, hair shampoo (100 g) containing the lactic acid bacteria fermented product of the sprout sprout extract prepared in Example 2 was prepared according to a conventional method.

Raw material Content (g) Fermented product of lactic acid bacteria from germinated sprout extract 1.0 Arachidyl glucoside 4.5 ethanol 2.0 Butylene glycol 2.0 Citric acid 0.1 Phenoxyethanol 0.02 Purified water Balance

Claims (9)

Enhancement of gene expression of Claudin-1 or Involucrin by containing fermented product of Lactobacillus gasseri ( Lactobacillus gasseri SKB1102, accession number: KCCM12342P) from germination sprout extracts of broccoli, rapeseed, sunflower and barley Cosmetic composition for strengthening the skin barrier, characterized in that it has a function. delete delete delete The method of claim 1,
A cosmetic composition for strengthening skin barrier, characterized in that the fermented product of lactic acid bacteria of the sprout sprout extract has an anti-pollution function. The method of claim 5,
The fermented product has an anti-pollution function against at least one environmentally harmful factor selected from the group consisting of fine dust, yellow dust, vehicle exhaust gas, dioxin, benzopyrene and heavy metals contained in the atmosphere. Cosmetic composition. The method of claim 1,
The fermented product is a cosmetic composition for strengthening skin barrier, characterized in that it has a function of regeneration of skin cells against UV damage. delete delete

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