KR102004323B1 - Cpmposition for care of scalp disease comprinsing plant extract - Google Patents

Abstract

The present invention relates to a composition for a scalp disease care comprising a plant extract as an active ingredient, and a composition capable of improving the condition of the scalp and preventing or treating dandruff without side effects, and a composition such as a shampoo for preventing dandruff . To this end, the present invention provides a composition for scalp disease care comprising an organic solvent extract of each of broccoli, obtusa, obtusa, oats and tallow. In addition, the composition preferably further comprises an organic solvent extract of each of a fungus monolayer, a fowl fungus, and a sorghum.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a composition for scalp disease care comprising a plant extract as an active ingredient,

The present invention relates to a composition for a scalp disease care comprising a plant extract as an active ingredient, and a composition capable of improving the condition of the scalp and preventing or treating dandruff without side effects, and a composition such as a shampoo for preventing dandruff .

One of the skin diseases that occur frequently in modern people is skin disease caused by fungal infection. Dandruff is also a leading skin disorder caused by fungal infections. Dandruff is a phenomenon in which the epidermis is separated from the scalp in the form of rice bran and the keratin appears prominently. One of the normal bacteria of the skin, Pityrosporum ovale ; And the Malassezia furfur ( Malassezia furfur ) in recent years) is a major cause of the overgrowth of the fungus. In addition, studies have shown that excessive sebum secretion, hormonal imbalance, excessive proliferation of scalp cells, stress, environmental pollution, excessive diet, and the like can be caused by a combination of skin diseases such as dermatitis or psoriasis do. Malassezia furfur is found to be more proliferated in seborrheic dermatitis. This releases the acid that stimulates the skin, causing the skin to become inflamed. In addition, when excess dandruff is left unattended, alopecia may be caused by deterioration of the entire scalp condition.

Therefore, shampoo and hair cosmetic compositions having dandruff inhibiting action for preventing or treating dandruff can suppress the proliferation and activity of malassezia furfur which is recognized as a major causative agent of dandruff, inhibit inflammation on scalp, To maintain a healthy state of the material is required to contain.

Drugs and shampoos used to treat dandruff include chemicals such as zinc pyridione, ketoconazole, and selenium sulfide. Many studies have been made to replace natural substances that are harmless to the human body since they have excellent antibacterial activity in a small amount, while they may be absorbed into the body to cause undesired side effects. On the other hand, in the case of dandruff treatment-oriented shampoo, the incense and quality are lower than the general shampoo and the feeling is not good. Furthermore, there is a problem that the dandruff should be improved not only by the dandruff problem but also by the scalp condition.

Korean Patent No. 10-0776346 Korean Patent Publication No. 10-2015-0108640 Korean Patent No. 10-0174807 Korean Patent No. 10-0800534 Korean Patent No. 10-1496926

The present invention aims to provide a composition capable of improving the condition of the scalp and preventing or supplementally treating or treating dandruff without side effects. Particularly shampoo for preventing dandruff.

To this end, the present invention provides a composition for scalp disease care comprising an organic solvent extract of each of broccoli, obtusa, obtusa, oats and tallow.

In addition, the composition preferably further comprises an organic solvent extract of each of a fungus monolayer, a fowl fungus, and a sorghum.

In addition, the scalp disease may be a dandruff disease.

The organic solvent is preferably ethanol.

The present inventors have identified a variety of information on natural substances that may have anti-inflammatory and antifungal properties. Many experimental results on individual natural products were confirmed. On the basis of the concept designed for the purpose of the present invention, a simple experiment was conducted on the screening level in the case where there is no related document about the medicines necessary for attaining the object of the present invention by adding the knowledge of oriental medicine, A simple screening experiment was carried out for the purpose of demonstration. The combination of the results of the screening and the oriental medical ideas constituted a combination of natural substances and verified that there is a substantial effect on the combination of these substances.

The present inventors have found a combination in which the antimicrobial effect against malassezia furfur is elevated while studying a combination of natural substances which are more effective in preventing or treating skin candidiasis or flocculant diseases. To test the antimicrobial effect of Malassezia furfur against individual substances and to take into account the oriental medicine balance, the basic composition of broiler, whitish, obovate, oat, and oil extracts is basically constituted. For their synergy, , And ginseng root extract. Gwangmumcheoncho, royal foliage epidemic, Gwangsonghyang extract can also improve the scalp environment.

Broiler (cinnmomi Cortex Spissus) is a dried bark of Cinnamomum loureirii or cinnamomum cassia, which is an evergreen aromatic tree belonging to the genus Lauraceae. It is harvested between October and October and shaded. It heals in the gods, the rain, and the cysts, and is used for the blood vessels of the blood vessels, the sorrows, abdominal pain diarrhea, The broiler chickens are composed of 1 ~ 3.4% of essential oil, 2 ~ 3% of tannin, mucus and carbohydrate, and the essential components of cinnamaldehyde are 75 ~ 90% of cinnamaldehyde, Tartrate, phenylpropylacetate, and silicodaldehyde. It enters the kidneys, the rain, the bladder, replenishes the human body's stomach, warms the stomach, removes the cold air, and works well for blood vessels. It is used as an agent for sweating, fever, analgesic. According to pharmacological tests, broiler chickens are known to have arthritis, antimicrobial activity and blood circulation.

Houttuyniae Herba is a perennial plant belonging to Saururus chinensis, whose scientific name is Houttuynia Cordata and has other names such as troches, It contains active ingredients in the entire leaf, stem, and root, and its physiologically active substances, including its unique odor, help metabolism, purify blood, promote kidney function, and excrete inflammatory symptoms and toxins. It has been used extensively as a civilian medicine in China, Japan and other countries since about 3000 years ago. The herb is used in hypertension, constipation, diabetes, fever, swelling, detoxification, arthritis, inflammatory diseases and skin diseases. The main ingredients are decanoyl acetaldehyde, melon, and myrcene, which are essential oils in the outpost. Has been widely used as a medicinal herb for various pharmacological actions such as diuretic action, analgesic action, hemostatic action, antimicrobial action, antiviral action, anti-vasodilatory action and anti-inflammatory action, and is one of promising health food materials together with Saururus chinensis. Specifically, it is used for the treatment of antipyretic, detoxification, pneumonia, malaria, tree species, hemophilia, hemorrhoids, skin diseases and the like, antimicrobial, diuretic, analgesic, hemostasis and promoting tissue regeneration. Recently, antioxidant helper simplex virus type 1 herpes simplex virus type 1), human immunodeficiency virus type 1 and influenza virus, antitumor, anti-leukemia, hyperlipidemic effect, toxicity suppression effect on cadmium, And the effects of Rhizoctonia sulphate on the metabolism of mobenzene.

Galla Rhois is an insect house made by insect parasitic on the leaves of rhus javanica L. (Rhus javanica L.) and is also called anchovy (白蟲 倉 倉). Dried insects on the leaves of rhusmia, a deciduous tree belonging to the Rhus verniciflua family, are used. Major ingredients include gallotannin, resin, fat, and starch. In pharmacological action, the gallotannin component of the dwarf has a converging effect. In addition, it has an antimicrobial action on alien bacteria, pseudomonas aeruginosa, etc. and exhibits liver function-protecting action and antioxidative action. In clinical studies, 15 grams of this drug was combined with GAZI for gastrointestinal hemorrhage and showed effective response when taken in combination with ulcerative colitis, radioactive rectitis, wells, pulmonary tuberculosis, hypertension, diarrhea, diarrhea, diabetes, , Hemorrhoids, burns, premature ejaculation, fever (啼 啼), pediatric diarrhea, and seasonal influenza are reported. Contains 50 to 60% of tannin.

Mume Fructus will smoke as the less ripe fruit of the plum tree Prunus mume Siebold et Zuccarini (Rosaceae Rosaceae). Fruit is spherical or flat spherical, 2 ~ 3cm in length and 1.5 ~ 2cm in diameter. Outer surface is black to dark brown, wrinkled, and there is a circular fruiting mark on the underside. The nucleus is very hard, elliptical, yellowish brown and has many concave dots on the surface, with a length of 1 to 1.4 cm, a width of 1 cm, and a thickness of 0.5 cm. The seeds are flat egg-shaped and light yellow. There is a peculiar smell and the taste is good. Omakawa stops the cough and seawater, stops the diarrhea, uses it for the thirst caused by lack of essence, and causes vomiting and abdominal pain caused by roundworm. It is also effective in indigestion and poor food. Plum, the flower of the plum tree, is a nervous irritant. It has effects on the symptoms such as chest tightness, non-digestion, foreign substances in the neck, 癩 郁 毒毒. Immunity enhancement, antimicrobial activity, and cervical cancer suppression have been reported by pharmacological actions of oats.

Sanguisorbae Radix is a bitter taste with dried syrups (Sanguisorba officinalis Linne) or dried roots of long leaves. It has the effect of hemostasis, convergence, and detoxification, and is effective for colitis, dysentery, diarrhea, hematemesis, eczema. Contains glycosides called Sanguisorbigenin and has components such as tannin, uric acid, ellagic acid, and fumaric acid. The crude oil extract from Oriental medicine is used for hemostasis, postpartum hemorrhage, and inflammatory menstrual hyperplasia because of its anti-inflammatory effect and vasoconstrictor effect.

On the other hand, the skin, which is the largest organ of the human body, occupies about 16% of the total human body volume, is in direct contact with the external environment and protects the human body from many harmful factors that intrude into the human body such as temperature, humidity and ultraviolet rays. It plays an important protective role. However, with age, skin cells are damaged due to various pollutants, strong ultraviolet rays, stress and nutritional deficiency, cell proliferation is not properly performed, and wrinkles, elasticity loss, and keratinization occur in the skin.

Skin aging is largely divided into intrinsic aging and extrinsic aging (Cosmetics & Toiletries, 111: 31-37 (1996)). My human aging is characterized by a gradual decline in the physiological function of the skin as it ages,

(Arch Dermatol., 130: 87-95 (1994)), which is characterized by a clinically reduced elasticity, coarse skin texture, deep wrinkles and pigmentation. External aging refers to the aging phenomena caused by external factors such as ultraviolet rays, reactive oxygen species and stress. In order to inhibit or slow down the progress of skin aging, cosmetics or skin protectants containing various skin protecting ingredients such as retinol, retinoid, vitamin C, flavonoid and tocopherol are being developed. However, such a cosmetic or skin protecting agent has a weak potency in the final formulation and has a slight effect on skin irritation. The scalp is similar.

In order to improve the condition of the scalp, the present invention can additionally utilize a fiber bundle, a royal flush, and a browning line.

Desmodii Herba is a pre-plant of the two-plant Desmodium styracifolium, and its medicinal area is above the ground. The stem is cylindrical and has a length of about 1m. It is yellowish, covered with dense, soft hair, and the vagina is slightly tight. Leaves are horny, lobules are 1 ~ 3, round to round square, 2 ~ 4cm in diameter, the front end is slightly concave and the base is heart-like to gentle circle. The upper surface is yellowish green to grayish green with no hairs, and the lower surface has villi firmly attached to grayish white color. The taste is sweet and sour, and the quality is cool. Ingredients are Alkaloid, Flavonoid glycoside, etc., and are known to have efficacy in urinary infection, urinary stone, nephritis, cholecystitis, lacquer, pediatric marker, hematoma, jaundice.

Melandrii Herba (Melandrii Herba) is a dry, ground surface when ripe fruits and other related plants are ripe. Its branches are thin and convex, with branches and leaves facing each other and 20 ~ 40 cm in length. It smells grassy and tastes a little bit, and seeds are sweet at first. It is used for treatment of amenorrhea, milk sickness, milk sickness, and gingko hemorrhage because it has the efficacy of a sickle sickle, low milk sickness, Recent studies have reported that linarin, schaftoside, orientin, and homoorentin, known as antioxidants, are contained.

Gam - sung has traditionally been used in gastric, gastrointestinal, thoracic, gastrointestinal, vomiting, headache, and other conditions. Nardostachys jatamansi , NJ) has been used extensively as a tonic, stimulant, and anticonvulsant in several Asian countries, and has also been used to treat epilepsy, pathological excitement, tremor, and seizures (Bagchi, A., et al., Planta Med. , 57, 9697 (1991)). Various sesquiterpenes such as Jatamansic acid and Jatamansone, lignans, and neo lignans are present in the roots (Chatterji, A, et al., National Institute of Science Communication, vol. 5, pp 99-100 (1997); Arora, RB., Indian Council of Medical Research, vol. 51 (1965)). Gyeongsanghyeong self-functioning abdominal pain and thoracic pain caused by the window to relieve pain, and also applies to nervous stomach and headache. Each leg (脚气) is dipped in water to wash the lesion. It works on the central nervous system and has a sedative effect like the heel root. If the heart rate is not constant, heart rate control effect is remarkable, and smooth muscle spasm such as bronchus, small intestine, large intestine and uterus is released. Because of the efficacy of the dog's satellite diet, it controls the symptoms of malnutrition, poor appetite, and so on. It also makes fragrance with all spices. Water, which is rooted in ginseng, has been used for mental disorders, insomnia, blood disorders and circulatory system disorders (Uniyal, MR, et al., J. Res. Indian Med. 4, 83 (1969) (Li, p. Et al., Journal of Pharmacology Science, 93, 122-125 (2003)), which enhances perfusion and central nervous system (CNS) effects. In addition, alcohol extracts of ganoderma lucidum protect rats from thioacetamide induced liver injury (Bagchi, A., et al.).

On the other hand, in order to strengthen the health condition of the scalp, Hashuo, Chungho, Clive and Youngryung incense can be additionally used.

Polygonum Multiflori Radix is the root of Polygonum multiflorum Thunberg (Polygonaceae). It is known as a medicinal herb that strengthens the kidney function, enhances its tincture, darkens hair, and makes it live longer without disease. It improves the function of the liver, eliminates fatigue, fills the skin, strengthens the bones and muscles, and strengthens the heart, which is also effective in the case of nervous breakdown or insomnia. Lecithin, oxymethyl anthraquinone, fats and tannins. Lecithin stimulates the endocrine glands, promotes growth and assimilation, and is the most important nutrient in our body. It is also a source.

Artemisia apiacea Hance is a kind of mugwort belonging to the family Asteraceae, and is known in Korea as Leptospira. It is known to have more than 350 species in Korea, including rivers, riversides and coasts of China and Japan. It is traditionally effective for eczema, jaundice, fever, geothermal and analgesia. Chung Ho is said to be the highest among the horses that refer to mugwort, but only Chung Ho is called Ho (蒿). Cheongho treats the nausea, headache, and vomiting caused by schizophrenia and summer heat by turning off the hemorrhoids, tingling the uvula due to undergrowth, treating fever and symptoms of summer fever. It also makes the face look better and darkens the white hair. Pharmacological actions have been reported to inhibit pandemic, to inhibit hepatic damage, to lower blood pressure, to paracetamol, to inhibit skin fungi, to calmness, to genomes, to asthma, to bile secretion,

Caryophylli Flos is a dry clove of cloves belonging to cloves and has been used as a placebo, antimicrobial, and antifungal agent since ancient times as the efficacy of onion river, The clove has dark brown or dark red color and has a 10 to 18 mm long burns and a thick calyx on top of it with four and four membranous flowers with a characteristic odor strong, tasty and intense and later slightly paralyzing the tongue. The active ingredients are known as essential oils such as eugenol and acetyleugenol. Examples of flavonoids include rhamnetin, kaempferol, and higenamine as an alkaloid. In addition, it contains galotanic acid, β-cariopilene, 2-hydroxy 4,6-dimethoxy-5-methylacetophenone and the like. The pharmacological effects of clove include uterine contraction, contraction of animal extracts, analgesia by local anesthesia, adrenaline-antagonistic action in addition to the above-mentioned structure, antibacterial and antifungal activity, mucous membrane stimulation, genomic action, promoting biosynthesis of collagen fiber Is known. (Akinrimisi et al., West Afr. J. Pharmacol. Drug Res., 1975 2 (2): 127-131), antimicrobial activity (Ramanoelina , 1992, 53 (1): 217-226), antitumor activity through induction of glutathione S-transferase (Zheng et al., J. Nat. Prod., 1992, 55 (7): 999-1003), reverse transcriptase inhibitory activity of MMLV (moloney murine leukemia virus) (Suthienkul et al., Southeast Asian J. Trop. Med., Public Health, 1993, 24 (4): 751-755) (Pourgholami et al., J. Ethnopharmacol., 1999, 64 (2): 167-171) have been reported.

Lysimachia foenum-graecum Hance is a biennial plant of Primulaceae. Its stem is about 70cm tall. Leaves are alternate and compound leaf. In summer, flower stem comes out from leaf axil and a small butterfly-shaped flower blooms. It is used when there is a headache due to a cold caused by this cold affair. It treats the symptoms of bloating of the thoracic part with fragrance. It also shows analgesic effect on nasal congestion or toothache. It is known that Europe has its origin in Korea and has analgesic, anti-inflammatory and whitening effects in oriental medicine. Younglung fragrant belongs to Primulaceae and has traditionally been used as a herbal medicine for gingham headache, chest tightness, roundworm disease (herbaceous gangbang). According to the identification of ingredients of Youngryung incense, compounds such as eugenol, methylchavicol, etc. have been reported.

Meanwhile, the extraction solution of the present invention can be obtained by extraction with water or an organic solvent. Examples of the organic solvent include lower alcohol, acetone, chloroform, methylene chloride, ether, ethyl acetate, hexane and the like. As the lower alcohol, methanol, ethanol, propanol and butanol can be mentioned, and ethanol is most preferable. The extracted liquid can be used directly or by concentrating and / or drying. In the case of extraction using an organic solvent, an organic solvent such as methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide (DMF) (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof. The active ingredient of the herbal medicine may be extracted at room temperature or warmed under the condition that the active ingredient is not destroyed or minimized. Depending on the organic solvent to be extracted, the degree of extraction and the degree of loss of the active ingredient of the medicament may differ. Therefore, an appropriate organic solvent should be selected and used. The extraction method is not particularly limited, and examples thereof include cold extraction, ultrasonic extraction, and reflux cooling extraction. Concentration of the extract can be carried out by reduced-pressure concentration, reverse osmosis concentration, or the like. The post-concentration drying step includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying and the like. In some cases, a process may be added to pulverize the final dried extract. In addition, the extract may be subjected to an additional fractionation process. Preferably, the extract is suspended in distilled water, and the non-polar solvent soluble layer is extracted and separated by using a nonpolar organic solvent such as hexane, ether, dichloromethane, chloroform, ethyl acetate, or a mixed solvent thereof , It may be used by concentration and / or drying.

Of course, the most important thing in the present invention relates to the kind of natural product selected and its combination.

The present invention improves the condition of the scalp, and can prevent or supplement the dandruff without any side effects. In addition, the composition of the present invention can be applied to a product used as a shampoo for preventing dandruff which can be used freely in daily life.

FIG. 1 is a photograph showing the antibacterial activity against Malassezia furfur by Paper disc diffusion method.
Figure 2 is a tabulation of the results of the test of Figure 3;

Hereinafter, the present invention will be described in more detail through examples and test examples. It should be understood, however, that such embodiments and the like are used for illustrative purposes in order to facilitate understanding of the present invention, and the scope and scope of the present invention are not limited thereto

The raw materials used in the experiment were purchased from the wholesaler of Sehwadang Traditional Chinese Medicine in Gwangju Metropolitan City. Vegetables were purchased from the market, and the vegetables were purchased and dried for 48 hours in a hot air drier at 40 ° C.

<Extraction of individual substances>

After accurately weighing 600 g of each dried material, about 70% ethanol of about 10 times of the raw material was added, After shaking in a water tank for 48 h, the mixture was filtered through Whatman No 2, concentrated using a rotary vacuum concentrator (Heidolf, Geman), stored at -20 ° C, and then used. 1 g of the concentrated material was dissolved in 10 ml of distilled water, and a concentration of 100 mg / ml was used as a sample for the basic screening.

<Antioxidant Experiment>

Antioxidant effects on individual substances were tested. To the solution prepared by diluting each sample with EtOH in a 96 well plate, 0.2 mM of 1,1-diphenyl-2-picrylhydrazyl (DPPH) (EtOH) was added in a certain amount. After shaking for 10 seconds, the sample was incubated at room temperature for 30 minutes and absorbance was measured at 520 nm using a microplate reader. L-ascorbic acid was used as the reference drug. The antioxidant effect was compared with the absorbance of the control without the sample. The DPPH free radical scavenging activity of each sample was measured three times.

Vitamin C showed the greatest antioxidative effect, but the oil and broiler chickens showed very good antioxidative effect.

<Bacterial culture>

Malassezia furfur (ATCC 12078, Pityrosporum) was purchased from the Korean Culture Center of Microorganisms (KCCM). yeast extract, malt extract, Peptone, Dextrose, Agar, and olive oil, and cultured at 37 ℃ for 24 h for 5 times.

<Paper disc diffusion method>

For the basic screening, the antimicrobial activity of plant extracts was measured using Paper disc diffusion method. The bacteria were subcultured five times, and 0.1 g of bacteria was diluted with a liquid medium to a concentration of ¹ × 10 ³ , 200 were uniformly plated on a YM plate medium, the samples were absorbed twice in 50 mm diameter paper discs, After incubation at 37 ° C for 24 hours, the antimicrobial activity of the inhibitory rings around the discs was examined and their diameters were measured and expressed as the antimicrobial activity per mg of the extract.

In addition, minimal inhibitory concentration (MIC) was measured using the agar diffusion method as follows. That is, each water extract was sterilized with a 0.45 membrane filter, and then inoculated on a plate culture medium containing the test bacteria and cultured at 37 ° C for 6 hours. The concentration at which no growth of dicembolium was observed was determined by MIC.

The results are shown in Figs. Broiler chickens showed the best effect against Malassezia furfur, and also showed excellent effects in the case of Horseshoe chinensis, Hassuo oshi, Jiu yu and Hiba. FLCZ, which is the official treatment, showed relatively good effects, but broiler, osteochondrum, osteochondrum, and observer also showed excellent effects.

The extracts were selected considering the antioxidative effect on individual substances, the antimicrobial effect against malassezia furfur and harmonization of oriental medicine. Broiler chickens were the strongest ones, and were selected mainly on the basis of these. To improve the skin conditions such as skin activation, we have also opted for Gwanggaejeoncho, Kwangbu, and Gwangsanghyang. However, the combination of these results unexpectedly enhanced the antibacterial effect.

Others, Hsaseo, Cheongho, Clove, and Yeongrae are added to help strengthen the basic functions of the body and activate the body, thus helping the health of the scalp. The effects of these combinations were tested.

The combination ratio of the individual extracts can be appropriately adjusted from half to eight times based on the broiler. The stronger one is used in a relatively small proportion, one of which is broiler. In addition, it is better to use relatively less cloves and oriental scents. It can be said that it is possible to use it in large quantity, but it is preferable that it is used medicinally in the middle of Hashuo, Qingdao, and Oatsu.

&Lt; Example 1 >

The composition was prepared by mixing ethanol extracts at a ratio of 4, 5, 2, 4 and 4 of broiler 1 based on the ratio.

&Lt; Example 2 >

In addition to Example 1, a composition was prepared by adding ethanol extracts at a ratio of 2: 1 to 5: 1.

&Lt; Example 3 >

In addition to Example 1, a composition was prepared by adding ethanol extracts at a ratio of 1: 1 to 2: 1, 2: 1, 2: 1, and 1:

<Example 4>

In addition to Example 2, a composition was prepared by adding ethanol extracts at a ratio of 1: 1 to 2: 1, 2: 1, 2: 1, and 1:

<Antioxidant Experiment>

The antioxidant experiments were carried out on Examples 1 to 4 according to the methods of the foregoing experimental examples. The antioxidative ability of Examples 2 and 4 was superior to that of Examples 1 and 2, respectively. It is presumed that the combination of Gwanggaegumcho, Gwangbu, Ganso, and Gwangsonghyang increases the antioxidant capacity.

&Lt; Antibacterial activity test &

According to the method of the above-mentioned experimental example, the paper disc diffusion method, which is a brief method for Examples 1 to 4, was conducted to test the antibacterial activity. Malasejiafurupur also showed excellent overall results. In particular, Examples 2 and 4 showed better results than broiler chickens, which is the best result of the individual material test, showing a synergistic effect on malassezia furfur.

&Lt; Collagenase Expression Inhibitory Effect >

The ability of the examples to inhibit collagenase expression was measured in comparison with EGCG. EGCG is an antioxidant that is known to regenerate epidermal cells of the skin and prevent aging of the skin. Human fibroblasts were plated at 5,000 cells per well in a 96-well plate containing DMEM medium containing 2.5% urea serum and cultured to 80% growth. Example and EGCG were treated at a concentration of 20 占 퐂 / ml for 24 hours, and cell culture medium was collected. The degree of collagenase production of the cell culture broth was measured using a commercially available collagenase measuring device. First, the cell culture solution collected on a 96-well plate uniformly coated with collagenase antibody was added and the antigen-antibody reaction was performed in a thermostatic chamber for 3 hours. Subsequently, the second collagen antibody conjugated with chromophore was added to a 96-well plate, and color development was induced at room temperature for 15 minutes. Then, 1 M sulfuric acid was added to stop the color development reaction. The color is yellow, and the degree of yellowishness varies depending on the extent of the reaction. Absorbance was measured at 405 nm, and the absorbance of the treated group was divided by the absorbance of the untreated group and the degree of expression was examined. The lower the degree of expression of collagenase, the higher the ability to inhibit the expression of collagenase and the less collagen decomposition in the skin, the less elasticity of skin elasticity will be inhibited and the amount of wrinkles to be formed will be reduced.

When the degree of expression of the untreated group was taken as 100, EGCG was 75%, and in the examples, it was somewhat lower than that of EGCG, and Example 4 was the best, followed by Example 2, The procedure of Example 3 was shown. It is believed that addition of substances such as sewage syrup is meaningful because of the significant difference in the effect when comparing Example 3 and Example 1.

&Lt; Elastase activity inhibitory effect >

The elastase activity inhibition of the Examples was measured in comparison with EGCG. Cannell RJP, Kellan SJ, Owsians AM, and Walker JM. Results of a large scale screen of microalgae for protease inhibitors were obtained from Planta Med. 1988; 54 (1): 10 -14). The amount of pnitroanilide produced from the substrate at 37 ° C for 20 minutes was measured at 445 nm using N-succinyl- (L-Ala) 3-p-nitroanilide as a substrate. 0.5 ml of each test solution was added to 0.5 ml of each test solution. 0.5 ml of porcine pancreas elastase (2.5 U / ml) dissolved in 50 mM tris-HCl buffer (pH 8.6) N-succinyl- (L-Ala) 3-p-nitroanilide (0.5 mg / ml) dissolved in HCl buffer (pH 8.6) was added for 20 minutes. Elastase inhibitory activity was determined in accordance with (absorbance of 1-treated group / absorbance of untreated group) * 100.

When the inhibition rate of the untreated group was regarded as 0%, EGCG showed 21%, and all of the examples showed more excellent effects. Example 4 was the best, followed by Example 2, but not much difference.

The antimicrobial effect against dandruff was doubled as well as the improvement of the skin condition of the extracts of Gwanggimcheon, Kwangwol, and ginseng extract was observed.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the scope of the present invention as defined by the appended claims. It should be understood that it can be performed within a range.

Claims (4)

The present invention relates to an extract of ethanol from each of broccoli, obtusa, obtusa, obtusa, jiu,
400 parts by weight of an adult chick, 200 parts by weight of an oboe, 200 parts by weight of oats, 400 parts by weight of crude oil, 200 parts by weight of light fungus, 200 parts by weight of royal flush, 100 parts by weight of persimmon, 200 parts by weight of sucrose, 100 parts by weight of Cloze, and 100 parts by weight of Youngran's fragrance. delete delete delete

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