KR101989654B1

KR101989654B1 - Beverage composition comprising salicornia herbacia extacts

Info

Publication number: KR101989654B1
Application number: KR1020150190788A
Authority: KR
Inventors: 김미향; 김수진; 이주영; 천지현
Original assignee: 신라대학교 산학협력단
Priority date: 2015-12-31
Filing date: 2015-12-31
Publication date: 2019-06-17
Also published as: KR20170079814A

Abstract

The invention Lemon Myrtle (Backhousia citriodora ) as an active ingredient and a composition for prevention or improvement of wrinkles containing an active ingredient of a beverage composition or a lemon myrtle extract having an anti-wrinkle preventing or remedying ability. The present invention confirms the antioxidative ability and collagen synthesis promoting ability of the lemon myrtle extract and is completed based thereon. In addition, in the case of the beverage containing the lemon myrtle extract, the present invention is not suitable for beverage production due to the bitter and bitter taste despite the lemon flavor peculiar to lemon myrtle. And the kind and content ratio of the additional ingredient were confirmed and completed on the basis of the result. The beverage thus prepared exhibited antioxidative activity and anti-wrinkle preventing or improving ability, and also exhibited excellent taste when it was consumed.
In view of the above, the beverage composition of the present invention has excellent antioxidative and collagen production promoting ability and is excellent in preventing or improving the wrinkle of skin and is excellent in beauty-improving effect in relation to the lemon myrtle extract, The present invention is widely used as a beverage composition exhibiting functionalities relating to skin care, since natural flavors which are not bitter and bitter and have excellent lemon-like flavor, excellent in terms of preference and harmless to the human body are included as active ingredients .

Description

[0001] BEVERAGE COMPOSITION COMPRISING SALICORNIA HERBACIA EXTACTS [0002]

The present invention relates to a functional beverage composition having a skin wrinkle-reducing ability, specifically a skin wrinkle-improving ability and an antioxidant ability.

According to a recent survey, the amount of beverages consumed by one Korean citizen per year is about 46 bottles per 1.5L PET bottle. The consumed beverage was mainly carbonated beverage, and other juice beverage was found to be main. On the other hand, other beverages in niche markets, which are surely palatable, had a dramatic market expansion of about 43% in 2001 compared to the previous year, and their growth rate is much faster than the carbonated drinks and juice market. Of the market.

In particular, functional beverages belonging to other beverages have been growing rapidly in line with international events and marketing, and have recently become a living beverage with growing interest in living sports.

Studies on the above-mentioned functional beverages are mainly focused on research on sports drinks. For example, in addition to the basic functions of water and electrolyte diffusion of existing ionic drinks, a high-function sports drink having a high functionality, specifically, a high-function role such as muscle formation and mobility improvement is being developed. Such highly functional sports drinks may further contain alginic acid, carnitine, taurine, or branched amino acids in addition to saccharides and electrolytes depending on the characteristics and uses of the product.

However, studies on functionality related to skin beauty and the like as other functionalities other than the sports related functions have been studied. Recently, as the beauty market related to skin beauty has been expanded in scale, the development and research of such beverages have been extensively studied in spite of the demand for functionalities such as skin function, It was confirmed to be limited.

In this regard, human skin is the largest organ of the human body that protects the various organs inside the skin from the outside, and it carries various physiological roles. However, not only does the skin's physiological function deteriorate with age, its skin thickness becomes thinner and its function as a skin barrier deteriorates. In addition, external factors such as ultraviolet rays, air pollution, air conditioning and heating, excessive fatigue, stress, The skin is dried by the factor, and such skin dryness also causes the physiological function of the skin to be deteriorated, which causes skin irritation and damage to be easily caused even by an external small stimulus source.

Generally, during the growing period, it is possible to easily cope with the aging factors from inside and outside by various defense systems of the skin. However, as the age increases, the antioxidant system is destroyed by active oxygen species generated by ultraviolet rays, As a result, the defensive ability is significantly reduced. As described above, when oxidative stress is applied to the skin when the defensive ability is lowered, lipid peroxidation, protein oxidation, chain cleavage and abnormal cross-linking of collagen and elastin as elastic fibers, cleavage of hyaluronic acid chain, DNA oxidation Resulting in damage to the biocomponent components. Damage of the above-mentioned bio-constituting components causes skin sagging, roughness, dryness, and the like, which are initial symptoms of wrinkles of the skin, resulting in wrinkles of the skin.

In the past, cosmetics, which were thought to be products used by women, are now used not only as instruments of expressing beauty but also as daily necessities, regardless of whether they are male or female. As the industry develops, , There is a growing interest in functional products that expect special effects and efficacy.

Functional cosmetics currently recognized in Korea are whitening products that help to whiten skin, wrinkle-reducing products that help to improve wrinkles in the skin, and sunscreen products that help protect skin from skin burns and ultraviolet rays .

In general, such functional cosmetics are interpreted as cosmetics that affect not only the cosmetic effect but also the structure and function of the body, and prevent, improve and treat the cosmetics. In particular, recently, the proportion of sales of functional cosmetics has increased markedly from the total sales of cosmetics, and research has been actively conducted to search for new effective ingredients for the human body and to utilize them effectively for skin.

In particular, with the recent increase in the population of elderly people due to the aging of the population and the improvement of quality of life due to economic development, there is an increasing demand for products capable of suppressing or controlling skin wrinkles naturally associated with aging . Particularly, due to an increase in opportunities for social activities of the elderly population, the interest in the beauty of the aged population is increased, while the stimulation of the skin such as ultraviolet rays and the promotion of aging factors are increasing, Demand and usage of functional cosmetics have increased significantly.

Regarding the improvement of the wrinkles of the skin, the reason that the elasticity of the skin is lost and loosened, that is, the wrinkles are formed on the skin appearance, is largely divided into the generation of wrinkles by natural aging and the generation of wrinkles by exposure to ultraviolet rays (photoaging) The mechanism of wrinkling by two causes has not yet been clarified. There are two main types of skin aging closely related to wrinkles. Natural aging, such as wind, temperature, humidity, tobacco smoke and pollution, including chronological aging and exposure to ultraviolet rays over time There is extrinsic aging due to recall factors.

A common phenomenon found in association with the causes of the two wrinkles, namely, natural aging and skin changes due to photoaging is reduction and modification of collagen and elastin, the main constituent proteins in the dermis, and skin caused by reduction of hyaluronic acid, It is elasticity reduction. Particularly, according to the results of studies up to now, it has been known that the reduction and modification of collagen and elastin, which are main proteins in the dermis, are directly involved in the generation of wrinkles and the reduction of elasticity of skin.

The collagen is a major component of the extracellular matrix and is present in extracellular epilepsy as a major substrate protein produced in fibroblasts of the skin. It is also an important protein that accounts for about 30% of the total weight of bioproteins, and has a solid triple helix structure. Collagen forms most of the organic matter in the skin, tendons, bones and teeth, especially in the bones and dermis of the skin. In most other structures, it exists as a fibrous inclusion.

The main functions of the collagen are to impart strength and tension to the skin to secure the mechanical rigidity of the skin, to induce the resistance of the connective tissue, to bind tissue, to support cell adhesion, to cell division and differentiation (upon growth or wound healing of the organism) Induction, and the like. In addition, collagen plays an important role in wound healing and is known to promote the synthesis of collagen in the damaged epithelium, thereby allowing the wound to be quickly and without scarring.

Particularly, it has been reported that the reduction of collagen, which accounts for 90% of the dermal layer, is closely related to skin aging. More specifically, the synthesis and degradation of extracellular matrix such as collagen in vivo is appropriately controlled, but the progress of aging or irradiation with ultraviolet light promotes the expression of matrix metalloproteinase (MMP), an enzyme that degrades collagen And collagen decomposition by the collagenase degrades skin elasticity and wrinkles are formed.

In addition, various types of skin aging inhibitors known to date are known to have various mechanisms for inhibiting skin aging. Representative examples include collagen production promoters, elastin activators, hyaluronic acid production enhancers, free radical scavengers, cell proliferators, photoaging inhibitors, keratinocyte proliferation inhibitors, humectants, noxious active oxygen production inhibitors, and cell regeneration agents.

Also, as a method for improving skin wrinkles, there has been proposed a method of using a composition for skin external application such as cosmetic or ointment containing collagen. However, since it is difficult to absorb collagen, which is a macromolecule material, percutaneous absorption, it is impossible to expect a moisturizing effect or a wound healing effect, so that it can not exhibit essential skin function improvement and wound healing function It was pointed out.

In recent years, research has focused on natural materials derived from natural materials in conjunction with environmentally friendly hot winds, and it has been mainly concentrated on herbs and herbal medicines Are being studied on the materials used.

KR 10-0750870 B KR 10-0879283 B KR 10-1239417 B KR 20010010368 A KR 20030076829 A KR 20120000251 A KR 20140053226 A KR 20140021529 A

The present invention relates to a functional product which is not a sport-related function but has a function of skin-beauty-related function, specifically a wrinkle-improving or preventive ability, an excellent antioxidative ability, It is an object of the present invention to provide a beverage composition.

It is another object of the present invention to provide a cosmetic composition comprising a natural substance as an active ingredient, specifically a cosmetic composition for improving or preventing wrinkles related to skin care.

In order to achieve the above object, the present invention Lemon Myrtle (Backhousia citriodora ) as an active ingredient, and more particularly, to a beverage composition having an antioxidative activity and an anti-wrinkle preventing or improving ability, which comprises a lemon myrtle extract as an active ingredient.

The lemon myrtle extract may be a lemon myrtle hot water extract. Preferably, the lemon myrtle hot water extract is prepared by extracting at 90 DEG C for 30 minutes. More preferably, the dried lemon myrtle leaf is 0.05 To 0.5 parts by weight of the polyvinyl alcohol, and extracting the mixture at 90 DEG C for 30 minutes.

The beverage composition having antioxidative activity and wrinkle preventing or improving ability may further comprise sugar, food soda and citric acid in the lemon myrtle hot water extract solution, preferably 1% (w / v) of sugar in the lemon myrtle hot water extract, , 0.43% (w / v) of sodium hydroxide and 0.1% (w / v) of citric acid.

In addition, the present invention may relate to a method for producing beverages comprising an extract of lemon myrtle having antioxidant ability and skin wrinkle improving or preventing ability.

Preparing a lemon myrtle hot water extract to prepare a lemon myrtle hot water extract by adding 0.25 part by weight of a dried lemon myrtle leaf to 100 parts by weight of water and extracting the mixture at 90 DEG C for 30 minutes; And adding 10 g of sugar, 4.3 g of food grade and 1 g of citric acid based on 1 L of the lemon myrtle hot-water extract solution, and mixing.

In this aspect, the invention Lemon Myrtle (Backhousia citriodora ) hot-water extract as an active ingredient.

In another aspect, the invention Lemon Myrtle (Backhousia citriodora ) extract as an active ingredient.

As described above, the lemon myrtle extract of the present invention has an effect of improving skin wrinkles or prevention of skin wrinkles in relation to the skin cell regeneration ability and collagen synthesis performance, and the beverage containing the lemon myrtle hot water extract of the present invention is extracted By using lemon myrtle, which is used as food, and water solvent, which is the safest solvent, safety is assured against side effects, and extraction which can control the taste of bitter taste and bitterness The present invention can be widely applied to the beverage industry and the skin care industry. Thus, the present invention is very effective in industrial aspects.

FIG. 1 is a graph showing the cell growth ability of fibroblast (NIH3T3 cell), which is a skin-derived cell, according to the kind of each extraction solvent according to an embodiment of the present invention. And the vertical axis represents the degree of cell growth relative to the control group (%).
FIG. 2 is a graph showing the ROS elimination ability according to the Lemon myrtle hot-water extract and LPS treatment according to an embodiment of the present invention. In the horizontal axis, the Lemon myrtle hot-water extract, LPS treatment, (%) Of ROS measured against negative control.
FIG. 3 is a graph showing collagen aggregation performance according to the throughput of the lemon myrtle hot water extract according to an embodiment of the present invention. In the horizontal axis, the treatment concentration of the lemon myrtle hot water extract and the control group are shown separately, (%) &Lt; / RTI >

The invention Lemon Myrtle (Backhousia citriodora ) extract as an active ingredient.

The present invention also provides a beverage composition having an antioxidative activity and a wrinkle preventing or improving ability comprising the lemon myrtle extract as an active ingredient.

The lemon myrtle extract may be a lemon myrtle hot water extract. Preferably, the lemon myrtle hot water extract is prepared by extracting at 90 DEG C for 30 minutes. More preferably, the dried lemon myrtle leaf is 0.05 To 0.5 part by weight, further preferably 0.25 part by weight of the dried lemon myrtle blend to 100 parts by weight of water and extraction at 90 DEG C for 30 minutes.

The beverage composition having antioxidative activity and wrinkle preventing or improving ability may be one comprising, as an active ingredient, sugar, soda, and citric acid as an active ingredient in the lemon myrtle hot water extract solution. Preferably, 1% (w / v ), 0.43% (w / v) of sodium hydroxide and 0.1% (w / v) of citric acid.

In addition, the beverage composition for preventing or improving wrinkles may be one comprising, as an active ingredient, sugar, soda, and citric acid as an active ingredient in a lemon myrtle hot water extract solution, and preferably 1% (w / v) And 0.43% (w / v) of sodium hydroxide and 0.1% (w / v) of citric acid.

The invention further Lemon Myrtle (Backhousia citriodora ) extract as an active ingredient.

In the present invention, the term "effective component" means a component for exerting the effect of the present invention, for example, a component of a composition which provides prevention, improvement or supplemental effect of skin wrinkles or improvement of preference degree.

Also, in the present invention, concentration refers to an operation or a process of removing water such as liquid food having a high water content to increase the concentration of high blood. It also means the process of obtaining a concentrate. The concentration may be carried out by a method of evaporating water (evaporation concentration), a method of mechanically separating the ice produced by freezing the water as a solvent (freezing concentration), applying a pressure higher than the osmotic pressure of the concentrate using a semipermeable membrane A method of passing only water through a semi-permeable membrane (reverse osmosis concentration), and the like.

Further, in the present invention, the concentrate means a substance in which a solvent or the like is removed from a substance to increase the concentration of the main solid component, and in general, the liquid is removed from a solution or suspension such as juice. In the present invention, the concentrate means that the formulation in the concentrate is liquid, and is also referred to as an extract.

In the present invention, the term " Brix " is also referred to as a brick, and may be used to indicate the concentration of a beverage containing sugar in a unit or sugar content. 1Brix means 1 g of sugar contained in 100 g of grape juice.

Further, in the present invention, the functional food refers to a food group imparted with added value to function and express the function of the food by physical, biochemical, biotechnological, or the like to the food, a bio-defense rhythm control of the food composition, Means a food which is processed and designed so that the body control function regarding disease prevention and recovery is sufficiently expressed in living body. The functional food may include a food-acceptable food-aid additive, and may further comprise suitable carriers, excipients and diluents conventionally used in the production of functional foods. The functional food is intended to include functional beverages.

Further, in the present invention, beverage means a general term for drinking or enjoying a taste, and is intended to include a functional beverage.

In addition, in the present invention, the functional beverage is a functional beverage, which is imparted with a value added to the beverage so that the function of the beverage functions for a specific purpose by physical, biochemical or biotechnological methods, , Skin care, prevention and recovery of diseases, and the like. The intention is to include beverages having a skin-beauty-related function.

In the present invention, the cosmetic composition is a composition used in the human body for cleansing and fine-tinging the human body to add charm and change the appearance brightly, or to maintain or enhance the health of the skin and hair. Detergents and cosmetics cosmetics).

The inventors of the present invention have found that lemon myrtle extract, which has been cultivated in Australia, which is known as a plant having antimicrobial activity and antioxidant activity, In addition to the disclosed antioxidant activity, it was confirmed that the skin fibroblast cell line has excellent cell growth, ROS elimination ability and collagen production ability, and thus it has excellent wrinkle improvement or prevention ability. In terms of extraction yield and cell growth ability, It was confirmed that the hot water extract using water was superior to the alcohol extract.

In addition, in the process of preparing a beverage using the lemon myrtle extract, in order to improve the taste and bitterness of the lemon myrtle hot water extract, Extraction is the best, adding sugar, soda and citric acid as additional ingredients to mask the bitter and bitter taste of the lemon myrtle hot water extract and adding 1% (w / v) of sugar to the lemon myrtle hot- ), 0.43% (w / v) in the case of soda, and 0.1% (w / v) in the case of citric acid.

Hereinafter, the present invention will be described in more detail.

The lemon myrtle ( Backhousia citriodora ) is a shrub that lives in the tropical forests of Queensland, Australia. The leaf of the lemon myrtle is a lemon flavor, and is the most widely used spice material in Australia. Recently, it is also used as a raw material or a spice for herbal tea, confectionery, drinks, nutritional supplements and the like. In addition, the lemon myrtle-derived oil is the most fragrant and long lasting among many lemon-flavored oils, and is often used to make soaps, lip balms or perfumes. The lemon flavor of the lemon myrtle is attributed to the citral ingredient, and it contains about 90% of the lemon, unlike the lemon which contains about 3 to 10%, and has a stronger lemon flavor and aroma than the lemon, It has been reported that the bitter taste and bitter taste are strong. It is known that citral is a monoterpene-based aldehyde component that does not dissolve in water but mainly melts in alcohol or ether.

Although some studies related to antioxidative and antimicrobial effects of lemon myrtle have been carried out, it has not yet been studied in the world, and it is not well known to general consumers in Korea, and there is almost no research and patent using them .

The lemon myrtle, which is an object of extraction of the lemon myrtle extract, may be at least one selected from the group consisting of stem, leaf, root, flower and fruit, preferably lemon myrtle leaf.

The lemon myrtle extract may be one prepared by a conventional method for producing plant extracts. Specifically, the lemon myrtle extract may be a solvent extraction method, a soaking extraction method, a cold extraction extraction method, a warm extraction extraction method, an ultrasonic extraction method, A fractionator may be used.

The extraction solvent of the lemon myrtle extract may be water or organic solvent. The organic solvent may be a polar solvent, a nonpolar solvent or a mixture thereof. Examples of the organic solvent may include alcohol, diluted alcohol, hexane, methylene chloride, acetone, ethyl acetate, ethyl ether, chloroform or a mixture thereof. May be an alcohol having 1 to 5 carbon atoms, for example, methanol, ethanol, propanol, butanol, isopropanol, and the like. The alcohol diluted water may be an alcohol diluted with water at 50 to 99.9% (v / v). Preferably, the extraction solvent may be an edible drink or a water, more preferably an edible drink, a high extraction yield, and an excellent functionality. In this respect, the lemon myrtle extract may be a lemon myrtle water extract.

The extraction temperature of the lemon myrtle extract may be 50 ° C to 120 ° C, preferably 80 ° C to 100 ° C, more preferably 85 ° C to 95 ° C, and most preferably 90 ° C. The extraction time of the lemon myrtle extract may be 10 minutes to 60 minutes, preferably 20 minutes to 40 minutes, more preferably 30 minutes.

The functional beverage composition containing the lemon myrtle extract of the present invention as an active ingredient has been confirmed through antioxidant activity and collagen production ability, and as a result, excellent wrinkle prevention or improvement ability has been confirmed.

The functional beverage is a function related to the regulation of the biological defense rhythm and the beauty of the beverage group or the beverage composition to which the added value is imparted so that the function of the beverage functions and expresses the function of the beverage by using physical, biochemical, biotechnological techniques, Means a beverage which has been designed so that the body control function related to the prevention or improvement of skin wrinkles, disease prevention and recovery, etc. is sufficiently expressed in living bodies.

The functional beverage is not particularly limited to the other ingredients other than the functional ingredient as an essential ingredient in an indicated ratio, and may contain various flavors or natural carbohydrates as an additional ingredient.

The ratio of the additional ingredients may be selected from the range of 0 to 20,000 parts by weight based on 100 parts by weight of the total effective ingredients of the present invention. However, the control of the bitter taste and bitter taste of the lemon myrtle extract has the effect of the present invention May be included at specific rates for certain selected ingredients.

Specifically, the beverage composition having antioxidative activity and anti-wrinkle preventing or improving ability may further include sugar, soda, and citric acid as an active ingredient in the lemon myrtle hot water extract to control the bitter taste and bitter taste of the lemon myrtle extract .

The added active ingredients sucrose, food soda and citric acid were 1% (w / v), 0.43% (w / v) and 0.1% (w / v) citric acid based on the volume of the lemon myrtle hot- Lt; / RTI > For example, based on 1 L of the lemon myrtle hot water extract, 10 g of sugar, 4.3 g of food grade and 1 g of citric acid may be used.

In this respect, the beverage composition having antioxidative activity and anti-wrinkle preventing or improving ability may be one comprising lemon myrtle hot water extract, sugar, soda, and citric acid as an active ingredient. Preferably, the lemon myrtle hot- (w / v), 0.43% (w / v) soda, and 0.1% (w / v) citric acid.

The beverage composition for prevention or improvement of wrinkles may be one containing lemon myrtle hot water extract, sugar, soda, and citric acid as an active ingredient. Preferably, the lemon myrtle hot water extract is mixed with sugar 1% (w / v), 0.43% (w / v) sodium chloride and 0.1% (w / v) citric acid.

The extraction solvent of the lemon myrtle extract may be water or organic solvent. The organic solvent may be a polar solvent, a nonpolar solvent or a mixture thereof. Examples of the organic solvent may include alcohol, diluted alcohol, hexane, methylene chloride, acetone, ethyl acetate, ethyl ether, chloroform or a mixture thereof. May be an alcohol having 1 to 5 carbon atoms, for example, methanol, ethanol, propanol, butanol, isopropanol, and the like. The alcohol diluted water may be an alcohol diluted with water at 50 to 99.9% (v / v). Preferably, the extraction solvent may be water. In this respect, the lemon myrtle extract may be a lemon myrtle water extract.

The extraction temperature of the lemon myrtle extract may be 30 ° C to 120 ° C, preferably 70 ° C to 100 ° C, and more preferably 80 ° C to 95 ° C. The extraction time of the lemon myrtle extract may be 10 minutes to 60 hours or 30 minutes to 30 hours or 1 hour to 10 hours.

Further, the extract extracted with the solvent may be further fractionated with any one solvent selected from the group consisting of hexane, methylene chloride, acetone, ethyl acetate, ethyl ether, chloroform, water, and mixtures thereof. Two or more kinds of solvents may be used for the fractionation, and the solvent extracts may be sequentially used or mixed according to the polarity of the solvent.

The thus-prepared extract or the fraction obtained by performing the fractionation process may be filtered, concentrated or dried to remove the solvent, and may be subjected to both filtration, concentration and drying. Specifically, the filtration can be performed using a filter paper or a vacuum filter, and the concentration can be reduced by using a vacuum concentrator, for example, a rotary evaporator. The drying can be performed by, for example, freeze drying.

As described above, it is possible to protect skin cells, to induce collagen synthesis in skin cells, to remove reactive oxygen species (ROS) in skin cells, to be excellent in preventing or improving wrinkles, The cosmetic composition of the present invention can be applied to skin requiring a wrinkle-improving activity, since it is safe and safe from side effects because it is an active ingredient.

Therefore, the cosmetic composition of the present invention can be a cosmetic composition for improving wrinkles, a cosmetic composition for preventing or improving wrinkles, or a cosmetic composition for anti-wrinkles.

The cosmetic composition may contain 0.0001% by weight to 50% by weight, preferably 0.001% by weight to 30% by weight of the extract of the lemon myrtle extract, preferably lemon myrtle hydrothermal extract, on the whole composition. That is, the content of the lemon myrtle extract should be at least a minimum content in order to achieve the skin-related functionality, specifically the wrinkle-preventing effect. However, considering the possibility of application to various formulations, And can be freely adjusted within a predetermined range. At this time, the content of the active ingredient and the content of the additional ingredient may be appropriately adjusted within the above range depending on the content of the ingredients contained in the formulation or cosmetic composition.

The cosmetic composition of the present invention can be used for the external ointment for skin, basic cosmetics, makeup cosmetics, body cosmetics or shaving cosmetics. Examples of the basic cosmetics include essences, lotions, creams, gels, lotions, Cream, emulsion, etc. Examples of the make-up cosmetic may include a foundation, a lipstick, an eye shadow, an eyeliner, a mascara, an eyebrow pencil and a makeup base. Examples of body cosmetics include soaps, liquid cleansing agents, , Sunscreen cream, sun oil, and shaving cosmetics include aftershave lotion and shaving cream.

The cosmetic composition of the present invention may contain, as an effective ingredient, a lemon myrtle extract, preferably an extract of lemon myrtle hot water extract, in addition to a functional substance having a wrinkle-improving effect or a specific function such as a whitening effect, , A diluent, and the like may be further included.

For example, the cosmetic composition may further contain a perfume, a pigment, a bactericide, an antioxidant, an antiseptic, a moisturizer, a thickening agent, an inorganic salt, or a synthetic polymer material to improve physical properties. An organic pigment, an organic pigment, an ultraviolet absorber, a pH adjuster, an alcohol, a blood circulation accelerator, a cold agent, a limiting agent or purified water may be used as a blending component which may be added.

The cosmetic composition may further include at least one selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

The components other than the above-mentioned effective components may be added within the range that does not impair the objects and effects of the present invention.

The cosmetic composition may be in the form of a solution, an emulsion, a viscous mixture or the like, and may be in the form of a liquid, a cream, a lotion, a pack, a foundation, a lotion, a serum, Lt; / RTI >

More particularly, the cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritional cream, a moisturizing cream, a hand cream, a foundation, , Packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

For example, when the formulation of the present invention is a paste, a cream or a gel, an animal fiber, a plant fiber, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc or zinc oxide Can be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as the carrier component. In particular, when the formulation of the present invention is a spray, Propane / butane, or dimethyl ether. &Lt; RTI ID = 0.0 >

When the formulation of the present invention is a solution or an emulsion, a solvent, a solvent, or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

In another aspect, the present invention relates to a method for producing beverages comprising an extract of Lemon Myrtle having antioxidant ability and skin wrinkle improving or preventing ability.

Preparing a lemon myrtle hot water extract to prepare a lemon myrtle hot water extract by adding 0.25 part by weight of a dried lemon myrtle leaf to 100 parts by weight of water and extracting the mixture at 90 DEG C for 30 minutes; And a lemon myrtle extract beverage preparation step wherein 10 g of sugar, 4.3 g of food grade and 1 g of citric acid are added and mixed on the basis of 1 L of the lemon myrtle hot water extract solution.

The step of preparing the lemon myrtle hot water extract solution is performed by adding 0.05 to 0.5 parts by weight, preferably 0.2 to 0.3 part by weight, of the dried matter of lemon myrtle leaves to 100 parts by weight of water and heating at 80 to 100 ° C, preferably 85 to 95 ° C, More preferably 90 ° C for 10 minutes to 60 minutes, preferably 20 minutes to 40 minutes, more preferably 30 minutes.

The lemon myrtle extract is prepared by adding 1% (w / v) sugar, 0.43% (w / v) sodium chloride to the lemon myrtle hot water extract prepared in the step of preparing the lemon myrtle hot water extract, And 0.1% (w / v) citric acid. For example, 10 g of sugar, 4.3 g of food grade, and 1 g of citric acid are added and mixed on the basis of 1 L of the lemon myrtle hot water extract.

Hereinafter, preferred embodiments of the present invention will be described. The following examples are for illustrative purposes only and are not intended to limit the scope of the invention.

Manufacturing example : Lemon myrtle Preparation of extract

The lemon myrtle leaves for the production of the active ingredient of the present invention, lemon myrtle leaves, were cultivated in the vicinity of Hadong Jiri Mountain in Kyungsangnam-do, and the dried lemon myrtle leaves were obtained from a domestic distributor, Pure Land. The lemon myrtle leaves were pulverized using a pulverizer.

The extract was prepared by adding distilled water (DW) or 1 L of 80% ethanol to 10 g of the lemon myrtle leaf extract and extracting the extract at 80 ° C for a total of 4 hours. The extract was filtered twice using a No. 4 filter paper (Whatman, UK) 90 mm in diameter, concentrated using a rotary vacuum evaporator, and freeze-dried using a freeze-drying device.

The extraction yields of the lemon myrtle hot water extracts were 15.2% and the extraction yields of lemon myristate ethanol aqueous solution were 9%, respectively. As a result, the extract yield of lemon myrtle hot water extract Was about 1.7 times higher than that of the conventional method, and it was confirmed that the hot water extraction method was more preferable as the extraction method.

Experimental Example : Lemon myrtle The functionality of the extract and Lemon myrtle Determine optimal combination ratio for extract preparation

Experimental Example 1-1. Lemon myrtle Antioxidant activity of extracts (1) - DPPH Scatters Measure

1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was measured by the method of Blois to determine the antioxidant activity of lemon myrtle extract by measuring the reducing power of DPPH radical of lemon myrtle hot water extract .

Specifically, the extract prepared in Preparation Example was dissolved in ethanol, and 150 μM DPPH dissolved in ethanol was mixed and reacted for 30 minutes. Then, the absorbance was measured at 540 nm using an ELISA reader. The measurement result was calculated by the following equation 1, and DPPH scavenging ability was confirmed. A in the following equation 1 denotes the absorbance of the sample, and B denotes the absorbance of the reaction solution (without sample). As a control group, ascorbic acid (Vit C) was used instead of the sample, and the results are shown in Table 1.

[Equation 1]

DPPH scavenging ability = (1 - A) / B X 100 (%)

density
(μg / ml) DPPH scavenging ability (%) Lemon Myrtle Hot Water Extract Vit C One 42.2 ± 2.5 55.5 ± 3.2 50 90.6 ± 7.1 92.9 ± 8.1 100 93.6 ± 5.2 95.8 ± 7.2 500 96.5 ± 5.8 95.4 ± 5.4 1000 97.3 ± 7.3 95.5 ± 8.7

As shown in Table 1, in the case of lemon myrtle hydrothermal extract, the activity was close to the activity of ascorbic acid (vitamin C, Vit C), which is a positive control, at a concentration of 50 ug / mL or more. Especially, it showed 97.3% at 1,000 ug / mL concentration of lemon myrtle hydrothermal extract and higher than ascorbic acid (vitamin C, 95.5%) as a positive control.

Experimental Example 1-2. Lemon myrtle Antioxidant Activity of Extracts (2) - SOD Similar activity Measure

In order to further confirm the antioxidant activity of lemon myrtle extract, the amount of pylogallol which catalyzes the conversion of superoxide dismutase (SOD) -like activity to H ₂ O ₂ by the method of Marklund et al. Respectively.

Specifically, the extract samples prepared in Preparation Example were diluted by concentration, and 10 μl of the sample was diluted with tris-HCl buffer (50 mM tris [hydroxymethyl] amino-methane, 10 mM EDTA, pH 8.5) Pyrogallol was added and the reaction was allowed to proceed at 25 ° C for 10 minutes. Then, 1N HCl was added to stop the reaction. After the reaction, the amount of pyrogallol was measured at 420 nm using an ELISA reader. The result of the measurement was calculated by the following equation 2 to confirm the SOD-like activity. A in the following equation 2 means the absorbance of the sample, and B means the absorbance of the reaction solution (without sample). As a control group, ascorbic acid (Vit C) was used instead of the sample, and the results are shown in Table 2.

[Equation 2]

SOD-like activity = (1 - A) / B X 100 (%)

density
(μg / ml) SOD-like ability (%) Lemon Myrtle Hot Water Extract Vit C One 14.6 ± 3.2 13.5 ± 2.6 50 16.8 ± 2.8 19.7 ± 7.5 100 20.5 ± 2.2 24.6 ± 4.6 500 27.6 ± 4.7 49.2 ± 6.1 1000 31.0 ± 6.3 65.5 ± 3.7

As shown in Table 2, lemon myrtle hydrothermal extract showed excellent SOD-like activity, and at 1 ug / mL or 50 ug / mL, similar degree to ascorbic acid as a positive control was confirmed. From the above results, it was confirmed that the lemon myrtle hot water extract had an excellent antioxidant activity.

Experimental Example 2-1. Lemon myrtle Extract Anti-wrinkle Active measurement (1) - Skin cell Proliferative ability Measure

Fibroblasts (NIH3T3 cells) were purchased from Korean Cell Line Bank in order to conduct skin cell experiments to confirm anti-wrinkle activity. The cells were cultured in a ₂ % CO ₂ incubator at 37 ° C and 5% CO ₂ using α-DMEM medium containing 10% FBS and 1% antibiotics. Respectively.

Cell proliferation was measured using Green 's MTT assay.

Specifically, the cultured NIH3T3 cells were plated in 96-well plates, and the lemon myrtle hot-water extract and lemon myrtle ethanol extract samples prepared at different concentrations were added and cultured at 37 ° C and 5% CO ₂ for 48 hours. After the incubation, the MTT reagent was treated, the medium was removed after culturing, and the insoluble formazan crystals produced by adding dimethyl sulfoxide (DMSO) were dissolved. The absorbance was measured at 550 nm with an ELISA reader. The cell proliferation rate is shown in Fig. 1 by calculating the absorbance of the lemon myrtle extract-treated group as a percentage of the absorbance of the control group.

As shown in FIG. 1, when treated with lemon myrtle hot water extract, NIH3T3 cells were significantly increased in cell number compared to the control without any sample, and the cell proliferation rate was 120% higher than that of the control. Was significantly increased compared with the lemon myrtle extract, which did not show any significant difference compared to the control group.

Experimental Example 2-2. Lemon myrtle Extract Anti-wrinkle Active measurement (2) - Skin cell of mine ROS Removability Measure

Regarding the ability to protect skin cells, ROS removal ability in skin cells was measured using NIH3T3 cells. The NIH3T3 cells cultured in Experimental Example 2-1 were distributed on a black 96-well plate, and then the lemon myrtle hot water extract, which was confirmed to have excellent cell protection ability in Experimental Example 2-1, was added at each concentration, CO ₂ for 48 hours. After the cultivation, lipopolysaccharide was added to induce active oxygen species, and further cultured at 37 ° C and 5% CO ₂ for 48 hours. Subsequently, the medium was removed, washed with PBS, and the ROS reagent was added to each well, followed by further incubation at 37 ° C and 5% CO ₂ for 1 hour and 30 minutes. After incubation, the medium was removed and fluorescence was measured at 485 nm (ex) and 535 nm (em) using a fluorescence spectrophotometer. The ROS removal ability was confirmed from the above measurement results and is shown in FIG.

As shown in FIG. 2, when the lemon myrtle hot-water extract was treated with NIH3T3 cells, the active oxygen was significantly removed and the degree of elimination was increased in a concentration-dependent manner. When 50 ug / ml was added, It was confirmed that the same amount of active oxygen was removed.

Experimental Example 2-3. Lemon myrtle Extract Anti-wrinkle Activity measurement (3) - Induction measurement of collagen production

With regard to the prevention or improvement of wrinkle, the ability to induce collagen production in skin cells was measured using NIH3T3 cells. NIH3T3 cells cultured in Experimental Example 2-1 were plated in 96-well plates and cultured at 37 ° C and 5% CO ₂ for 48 hours. After the incubation, the cells were washed twice with PBS, and then cells were obtained using a cell scraper. Then, 0.05 M Tris-HCl buffer was added, and the cells were further suspended and sonicated with an ultrasonicator . Then, 5% TCA was added, followed by centrifugation. The supernatant was removed, and then 5% TCA was added to the precipitate again. Thereafter, the supernatant was removed, the precipitate was reacted with 6 N HCl at 110 ° C for 20 hours, and the reaction was then filtered and concentrated, and the pH was adjusted to pH 7. The hydroxyproline content in the sample solution was measured using the Woessner method, and the collagen content from the amount of hydroxyproline obtained usually contained about 10% of hydroxyproline in the collagen Therefore, the following equation 3 was used. The conversion result is shown in Fig.

[Equation 3]

Collagen content = hydroxyproline content X 100/11

As shown in FIG. 3, the collagen synthesis promoting activity in NIH3T3 cells was significantly dependent on the concentration of lemon myrtle hot water extract in the concentration-dependent manner. Especially, at a low concentration of 0.1 ug / mL, the ability to promote high collagen synthesis was significantly higher than that of the control. At the highest concentration of 200 ug / mL, the collagen synthesis promoting ability was over 200% higher than that of the control. Collagen is the most important protein that constitutes the connective tissue. It is closely related to prevention or improvement of skin wrinkle formation in skin cells. When such a collagen production promoting ability is recognized, it is evaluated that skin wrinkle improvement or prevention ability is recognized.

Experimental Example 4-1. Lemon myrtle Extract Beverage preference (1) - Confirmation of added ingredient content

As described above, the extract of Lemon Myrtle having an antioxidative activity and skin wrinkle improving or preventing ability extracted with a water solvent which is an excellent solvent in terms of antioxidative activity and collagen production ability, that is, &Lt; / RTI > to prepare the beverage composition.

First, 1 L of distilled water (DW) was added to 10 g of the above-mentioned lemon myrtle leaf pulverized product based on the conditions for preparing the hot-water extract having excellent functionality in the functional test, and the mixture was extracted at 80 ° C for a total of 4 hours.

The optimum content ratio of the lemon myrtle leaf extract for masking the bitter, bitter and thick feeling of the lemon myrtle leaf extract was confirmed by adding sugar, soda, and citric acid, which are components added mainly to the ionic drink, . First, through several preliminary experiments, the content of citric acid was determined to be 0.1%, and the amount of sugar added was adjusted to impart sweetness to the bitter and bitter taste masking effect. More specifically, 1 g of citric acid and 17 g of soda were added to 1 L of the lemon myrtle leaf extract, and 1 g of the sugar (Example 1) and 10 g of the sugar Example 2) or 50g (Example 3) was added to prepare a beverage. Sensory evaluation of flavor, aroma and overall acceptability of the prepared beverage including whether or not the bean paste was masked was performed. The sensory test was carried out by 13 students who were trained in food and nutrition science students who were accustomed to the sensory test for food, and each sample was sampled. Each item was assessed by a 5-point scale method (5 points: very good, 4 points : Good 3 points: moderate, 2 points: normal, 1 point: poor), and the result of the sensory evaluation was obtained by averaging the above-mentioned evaluation results. The results are shown in Table 3. Statistical significance of the test results was analyzed by the ANOVA.

Experimental Example 1 Experimental Example 2 Experimental Example 3 flavor 2.7 3.5 2.8 incense 3.6 3.6 3.4 Overall likelihood 2.8 3.4 2.9

As shown in Table 3, Example 2 in which 10 g of sugar was added in three samples was found to have the highest preference. Specifically, the addition of sugar had little effect on the fragrance itself, and the fragrance itself was rated excellent in all examples due to the lemon fragrance. However, in case of the taste, Example 1 shows that the lemon flavor and bitter taste of the lemon myrtle extract The taste was hardly masked and the overall acceptability was low. In Example 3 in which 50 g of sugar was added, the lemon and bitter taste of the lemon myrtle extract was well masked, but added with excessive sugar, Respectively. Taking these results into consideration, with respect to the taste having a greater influence on the overall preference, the sugar for adding the bitter taste and the bitter taste of the lemon myrtle extract has no effect on the flavor, but is not suitable as a beverage in an excessive amount, It was confirmed that the addition of 10 g was preferable because a masking effect could not be exhibited when a small amount was added.

As a result of the questionnaire survey on the panel in the above experiment, there were many opinions that there is a feeling of slippery in the beverage, and the texture is decreased.

First, the amount of sugar added and the amount of citric acid added were determined to be 1% and 0.1%, respectively. From the results of the above tests, the sugar content and the citric acid content were determined to be 1.7%, 0.85%, 0.43%, and 0.21% 1 g of citric acid and 10 g of sugar were added to 1 L of lemon myrtle leaf extract, and further 17 g (Example 2), 8.5 g (Example 4), 4.3 g (Example 5) or 2.1 g Example 6) was added to prepare a beverage. Sensory evaluation of mouthfeel and overall acceptability of the prepared beverage was conducted mainly on whether or not the feeling of slipperiness can be controlled. The sensory test was carried out as described above, and the results are shown in Table 4.

Experimental Example 2 Experimental Example 4 Experimental Example 5 Experimental Example 6 Texture 2.5 2.7 3.1 3.1 incense 3.6 3.5 3.5 3.4 Overall likelihood 3.4 3.5 3.9 3.7

As shown in Table 4, it was confirmed that Example 5, in which 4.3 g of formula soda was added to the four samples, was the most favorable. Specifically, the addition of the food soda had little effect on the flavor itself, and thus it was evaluated in all the examples. However, in the case of the foodstuff, in the case of Example 2 in which 17 g of the formula was added or in Example 4 in which 8.5 g was added, , The texture was a problem due to the slippery feeling caused by the addition of the vegetable oil. In Example 6, in which 2.1 g was added, the user felt no cool feeling due to the addition of the soda, so that Example 5 containing 4.3 g was the most preferable.

Experimental Example 4-2. Lemon myrtle Determination of extract drink preference (2) - Determination of extraction method

After confirming the content of the additional ingredients, the degree of preference was measured by adjusting the production conditions of the lemon myrtle extract as the main raw material, that is, the extraction method, in order to secure the optimum degree of preference.

First, 10 g, 5 g, 2.5 g, and 1 g of lemon myrtle leaf pulverized product were added to 1 L of distilled water to control the content of the lemon myrtle leaf powder added at the time of extraction, And the degree of preference was evaluated. As a result of the above evaluation, when the content of lemon myrtle leaves was reduced, bitterness and bitter taste were reduced, but both of 2.5 g and 1 g were practically almost the same and bitter taste was almost the same. In the case of lemon flavor, There was no significant difference according to the addition amount of lemon myrtle leaf powder up to 2.5 g. However, when 1 g was added, the fragrance was somewhat lowered. Finally, it was confirmed that 2.5 g was most suitable to be added.

After that, 2.5 g of lemon myrtle leaf pulverization product was added to 1 L of distilled water, and then extraction process was carried out at different extraction temperatures to prepare a lemon myrtle leaf extract. Specifically, Experimental Example 7 (extraction at 30 占 폚 for 80 占 폚), Experimental Example 8 (extraction at 90 占 폚 for 30 minutes) and Experimental Example 9 (extraction at 108 占 폚 for 3 hours and 30 minutes) A control sample to which the additional components were added was prepared. Sensory evaluation was performed on the color, flavor, taste, flavor and overall acceptability of the prepared beverage. The sensory test was carried out by sampling 13 samples of a special panel consisting of students from Silla University Food and Nutrition Department trained to familiarize students with the sensory test for food, Table 5 shows the results. The statistical significance of the test results was tested using the ranking method significance test (score range 26-39, p <0.05, if there is a standard sample in the ranking method significance test).

Item color incense flavor cove Overall assessment Experimental Example 7 24 28 24 29 27 Experimental Example 8 20 17 20 20 21 Experimental Example 9 41 36 41 41 39 Control Example 45 49 45 40 43

As shown in Table 5, Experimental Example 8, which was extracted at 90 占 폚 among the four samples including the control sample, was found to have the highest preference. Specifically, when extracted at 100 ° C for 3 hours and 30 minutes, it was found that the extraction was excessive, the color was too dark, and the taste was too strong for the taste and the aftertaste. When extracted at 80 ° C, . Therefore, the taste of the sample of Experimental Example 8, which was extracted at 90 ° C, which was found to have the best taste and the most favorable taste, was not the best.

Manufacturing example : Functional beverage composition

Lemon Myrtle Hot Water Extract 1 L

10 g of sugar

4.3 g of sodium hydroxide

Citrate (Citrate) 1 g

Total Composition Volume 1 L

Although the components and the contents of the components and the contents of the components and the contents suitable for the beverage composition exhibiting the effects of the present invention are mixed and formulated as a preferred embodiment, the additional components including the electrolytes in addition to the components and the contents thereof, And then further mixed according to a conventional functional food manufacturing method.

Claims

A beverage composition for prevention or improvement of wrinkles comprising an extract of hot water of Lemon myrtle ( Backhousia citriodora ) as an active ingredient.

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The method according to claim 1,
The lemon myrtle hot water extract solution was prepared by adding 0.25 parts by weight of dried lemon myrtle leaves to 100 parts by weight of water and extracting at 90 캜 for 30 minutes,
The beverage composition for prevention or improvement of wrinkles is further characterized in that it further comprises 1% (w / v) sugar, 0.43% (w / v) sugar and 0.1% (w / v) citric acid in the lemon myrtle hot water extract
Wherein the beverage composition is a wrinkle preventing or improving beverage composition.

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The method of claim 3,
The beverage composition for preventing or improving wrinkles
Preparing a lemon myrtle hot water extract to prepare 0.25 parts by weight of a dried leaf of Backhousia citriodora against 100 parts by weight of water and extracting it at 90 캜 for 30 minutes to prepare a lemon myrtle hot water extract; And adding 10 g of sugar, 4.3 g of food grade and 1 g of citric acid based on 1 L of the lemon myrtle hot-water extract, and mixing
&Lt; RTI ID = 0.0 >
Wherein the beverage composition is a wrinkle preventing or improving beverage composition.

A cosmetic composition for prevention or improvement of wrinkles comprising an extract of Lemon myrtle ( Backhousia citriodora ) as an active ingredient.

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