KR101940055B1 - Composition for skin moisturizing containing extract of soybean leaf - Google Patents

Abstract

The present invention relates to a composition for antioxidation, anti-aging, skin regeneration, whitening or moisturizing comprising a soybean leaf extract. The composition of the present invention is useful not only to prevent oxidation of the body, to prevent aging but also to regenerate and regenerate skin cells, and has a skin whitening function. In addition, the composition of the present invention has a skin moisturizing function. In particular, the composition of the present invention shows superior effects to soybean extracts in antioxidant ability, anti-aging ability, skin regeneration ability, whitening ability or moisturizing ability. The composition of the present invention is also free from irritation to organisms including natural extracts obtained from natural products.

Description

Technical Field [0001] The present invention relates to a composition for skin moisturizing,

The present invention relates to a composition for antioxidation, anti-aging, skin regeneration, whitening or moisturizing comprising a soybean leaf extract.

The human skin undergoes changes due to various internal and external factors as it gets older. In other words, internally, secretion of various hormones that regulate metabolism is reduced, the function of immune cells and the activity of cells are lowered, and the biosynthesis of immune proteins and biocompatible proteins necessary for the living body is reduced, and ozone layer destruction As the amount of ultraviolet rays reaching the surface of sunlight increases, skin thickness decreases, wrinkles increase, elasticity decreases, and various changes such as spots, freckles, and black spots are also caused.

As the aging progresses, the content and arrangement of collagen, elastin, hyaluronic acid, and glycoprotein, which constitute the skin, are changed or decreased, and oxidative stress due to free radicals and active noxious oxygen is experienced. Cox-2 (cyclooxygenase), an enzyme that produces proinflammatory cytokines, known to cause inflammation in most of the cells that make up the skin by aging or ultraviolet light The biosynthesis of matrix metalloproteinase (MMP), which is an enzyme that decomposes the skin tissue by these inflammatory factors, is increased, and nitric oxide (NO) production by iNOS (inducible nitric oxide synthase) . In other words, decrease of cellular activity due to natural endogenous aging and decrease of biosynthesis of substrate material by microinflammation, increase of stress due to various harmful environment, increase of active oxygen species by sunlight, As the degradation and denaturation are accelerated, the skin substrate is destroyed and thinned, and various symptoms of skin aging appear. Therefore, it is a reality that many researches have been conducted on active ingredients that can prevent and improve such aging phenomena.

On the other hand, active oxygen generated by various physical, chemical, and environmental factors such as in vivo enzymatic system, reductive metabolism, chemical agents, pollutants and photochemical reaction, etc. is non-selective, irreversible It is known to cause various diseases including cancer or aging cells by destructive action. In addition, lipid peroxides and various peroxides such as lipid peroxides produced as a result of lipid peroxidation by these active oxygen also cause oxidative destruction to cells and cause various dysfunctions and cause various diseases. Therefore, antioxidants such as free radical scavengers or peroxide formation inhibiting substances are expected as agents for inhibiting or treating various diseases caused by these oxides.

Therefore, the inventors of the present invention found that when the soybean leaf extract was applied to the skin during the development of the material using the soybean, it could enhance not only the skin's antioxidant function, anti-aging function, skin regeneration function and whitening function, but also the moisturizing ability Thereby completing the invention.

Korean Patent Publication No. 1998-077876

It is an object of the present invention to provide a composition comprising a natural extract which can be used for antioxidation, anti-aging, skin regeneration, whitening or moisturizing.

In order to achieve the above object, the present invention provides a composition for antioxidation comprising soybean leaf extract as an active ingredient.

The present invention also provides an anti-aging composition comprising soybean leaf extract as an active ingredient, and provides a skin regeneration composition containing soybean leaf extract as an active ingredient and a whitening composition comprising soybean leaf extract as an effective ingredient.

The present invention also provides a moisturizing composition comprising soybean leaf extract as an active ingredient.

The composition of the present invention is useful not only to prevent oxidation of the body, to prevent aging but also to regenerate and regenerate skin cells, and has a skin whitening function. In addition, the composition of the present invention has a skin moisturizing function. In particular, the composition of the present invention shows superior effects to soybean extracts in antioxidant ability, anti-aging ability, skin regeneration ability, whitening ability or moisturizing ability. The composition of the present invention is also free from irritation to organisms including natural extracts obtained from natural products.

In this specification, the term 'bean' is not limited. In a composition according to one aspect of the present invention, the soybean of the present specification is selected from the group consisting of Seed Rice, Seomotei, Black Rice, Chungtae, Chrysanthemum, Fence, Soybean, Kidney Bean, But is not limited thereto.

In the present specification, 'canola' means the leaf part of the bean curd.

In the present invention, the 'canola leaf extract' is extracted in the extraction process by, for example, washing and drying the soap leaves or the kaolinized kaolin powder in water or an organic solvent, extracting it, and then filtering and separating the residue and the filtrate And the separated filtrate is concentrated under reduced pressure to obtain a soybean leaf extract. The organic solvent usable in the present invention may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform or a mixed solvent of these organic solvents and water. In view of the safety of raw materials, Of ethanol is used. After obtaining the extract by using the solvent, a liquid substance can be obtained by freezing, heating and filtering at room temperature by a conventional method known in the art. Alternatively, the solvent can be evaporated, spray dried or lyophilized But the present invention is not limited thereto, and any method generally used in the art can be used without limitation.

In one aspect, the present invention relates to a composition for antioxidation comprising an extract of soybean curd as an active ingredient.

As used herein, the term " antioxidant " refers to the ability to slow, prevent or prevent the oxidation process known in the art without limitation.

In another aspect, the present invention relates to an anti-aging composition comprising an extract of soybean curd as an active ingredient.

As used herein, the term " anti-aging " refers to an effect of slowing down, preventing or preventing the aging process known in the art. Specifically, it effectively inhibits collagenase expression in the skin, ≪ RTI ID = 0.0 > and / or < / RTI > improving wrinkles.

In another aspect, the present invention relates to a composition for regenerating skin comprising an extract of soybean curd as an active ingredient. The composition of the present invention can regenerate the skin by promoting the growth of the skin cells.

In another aspect, the present invention relates to a whitening composition comprising an extract of soybean curd as an active ingredient. Specifically, the composition of the present invention can provide an excellent whitening effect by inhibiting tyrosinase.

In another aspect, the present invention relates to a composition for moisturizing comprising an extract of soybean curd as an active ingredient. Specifically, the composition of the present invention promotes the differentiation of keratinocytes and can provide an excellent moisturizing effect.

In one aspect of the present invention, the composition may comprise from 0.001 to 10% by weight of soybean leaf extract, based on the total weight of the composition. If the content of the soybean leaf extract is less than 0.001% by weight based on the total weight of the composition, the antioxidant, anti-aging, whitening and moisturizing effect are insignificant, and if it exceeds 10% by weight, In view of the above, the composition of the present invention is used in an amount of 0.005 to 9.5% by weight, 0.01 to 9% by weight, 0.03 to 8.5% by weight, 0.05 to 8% by weight, 0.07 to 7.5% by weight, 7 to 7 wt%, 0.1 to 6.5 wt%, 0.3 to 6 wt%, 0.5 to 5.5 wt%, or 0.7 to 5 wt% of the soybean leaf extract.

In one aspect of the present invention, the soybean is selected from the group consisting of Seoritae, Glycin max MERR, Seomoktae, Rhynchosia Nolubilis , Black soybean, Glycine max (L.) Merr., Blue bean, Glycime max MERR), yellow bean ( Glycime max MERR), field bean ( Vicia faba ), kidney bean ( Phaseolus vulgaris ), pinto bean ( Phaseolus vulgaris L.), small red bean, Vigna angularis , small black bean, Phaseolus angularis WF WIGHT, sprouting bean, Glycine max (L.) Merr. and soybean ( Glycine max ) (Soybean, Glycine max ), but is not limited thereto.

In one aspect of the present invention, the composition may inhibit the production of reactive oxygen species (ROS).

In one aspect of the present invention, the composition may increase the expression of collagenase or the activity of collagenase.

In one aspect of the invention, the composition is capable of proliferating skin cells.

In one aspect of the present invention, the composition may inhibit the expression of tyrosinase or the activity of tyrosinase.

In one aspect of the present invention, the composition may promote differentiation of keratinocytes.

In one aspect of the invention, the composition comprises a cosmetic composition.

The cosmetic composition according to the present invention may be provided in all formulations suitable for topical application. For example, it may be provided as a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an oil phase in water, a suspension, a solid, a gel, a powder, a paste, a foam or an aerosol composition. Compositions of such formulations may be prepared according to conventional methods in the art.

The cosmetic composition according to the present invention may contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, so long as they do not impair the main effect. The cosmetic composition according to the present invention may further comprise a moisturizing agent, an emollient agent, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, a cold agent or a limiting agent. The compounding amount of the above components can be easily selected by those skilled in the art within a range not to impair the objects and effects of the present invention. The amount thereof may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight, have.

In one aspect of the invention, the composition comprises a pharmaceutical composition.

When the composition according to the present invention is applied to medicines, it may be formulated into an oral or parenteral dosage form in the form of solid, semi-solid or liquid by adding an inorganic or organic carrier to the composition as an active ingredient.

Examples of the agent for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups and pellets. Examples of the parenteral administration agent include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method. Surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffering agents, suspending agents and other adjuvants can be suitably used.

The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously and the like.

The effective ingredients of the pharmaceutical composition of the present invention will vary depending on the age, sex, weight, pathological condition and severity of the subject to be treated, administration route or judgment of the prescriber. Determination of the amount of application based on these factors is within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / / Day, but is not limited thereto.

In one aspect of the invention, the composition comprises a health food composition.

* The composition according to the present invention provides various types of food additives or functional foods containing them. The composition may be formulated into fermented milk, cheese, yogurt, juice, probiotics, tablets, granules, drinks, caramels, diet bars, and the like, and may be processed into ordinary tea leaves, tea bags, And can be used in the form of various other food additives.

In one embodiment, the composition may contain other ingredients or the like that can give synergistic effects to the main effect within a range that does not impair the intended main effect of the present invention. For example, additives such as perfume, coloring agent, bactericide, antioxidant, preservative, moisturizing agent, thickening agent, inorganic salt, emulsifier and synthetic polymer substance may be further added for improvement of physical properties. In addition, it may further contain auxiliary components such as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides and seaweed extract. The above components may be mixed and selected without difficulty by those skilled in the art depending on the purpose of formulation or use, and the amount thereof may be selected within a range that does not impair the objects and effects of the present invention. For example, the addition amount of the above components may be in the range of 0.01 to 5% by weight, more specifically 0.01 to 3% by weight, based on the total weight of the composition.

The composition according to the present invention may be in various forms such as a solution, an emulsion, a viscous mixture, a tablet, a powder, etc., and may be administered by various methods such as simple drinking, injection administration, spraying or squeezing.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

[Example 1] Production of soybean leaf extract

The soybean leaves were washed with purified water, dried and then hemihalized. 100 g of the bean leaf powder was placed in 1 liter of a 70% aqueous ethanol solution, boiled for 12 hours in an extractor equipped with a condenser, and then filtered through a 300-mesh filter cloth. Thereafter, the filtrate was treated with 4-15 And allowed to stand for 7 days at < RTI ID = 0.0 > 0 C < / RTI > for aging and then filtered through Watson # 2 filter paper. Add the above extract to a 3-liter separatory funnel, add 1 liter of ethylacetate, shake and mix, then take the upper layer (ethyl acetate layer) when complete separation into two layers. The lower layer (water layer) is extracted twice more with a separatory funnel. The separated upper layers were combined and concentrated under reduced pressure at 50 ° C using a distillation apparatus equipped with a cooling condenser and dried.

[Comparative Example 1] Preparation of soybean extract

The beans were washed with purified water, dried and then chased. 100 g of soybean flour was placed in 1 liter of a 70% aqueous ethanol solution, which was boiled in an extractor equipped with a condenser for 12 hours and then filtered through a 300-mesh filter cloth. Thereafter, the filtrate was treated with 4-15 And allowed to stand for 7 days at < RTI ID = 0.0 > 0 C < / RTI > for aging and then filtered through Watson # 2 filter paper. Add the above extract to a 3-liter separatory funnel, add 1 liter of ethylacetate, shake and mix, then take the upper layer (ethyl acetate layer) when complete separation into two layers. The lower layer (water layer) is extracted twice more with a separatory funnel. The separated upper layers were combined and concentrated under reduced pressure at 50 ° C using a distillation apparatus equipped with a cooling condenser and dried.

[Test Example 1] Inhibitory effect on reactive oxygen species (ROS) production

Keratinocytes isolated from human epidermal tissue were added to each well of a 24-well plate at 5 × 10 ⁴ for 24 hours. After 16 hours, the bean leaf extract was treated with 1%. For comparison, the soybean extract was treated with 1% and the control group was not treated with the soybean leaf extract. After 2 hours, the culture solution was removed, and 100 μl of phosphate buffered saline (PBS) was added to each well. This keratinocyte was irradiated with ultraviolet rays of 30 mJ / cm 2 using an ultraviolet B (UVB) lamp (Model: K5T8, UVB 15 W, Sankyo Dennki, Japan), and then PBS was removed. The keratinocyte culture medium 200 ≪ / RTI > The soybean extract and soybean extract were treated again and the amount of active oxygen species increased by ultraviolet stimulation was determined at predetermined time intervals. The amount of ROS was determined by reference to Tan's method of measuring the fluorescence of DCF-DA (dichlorofluorescin diacetate) oxidized by ROS (Tan et al., 1998, J. Cell Biol. Vol. 141, pp1423-1432) The ratios of the vehicle-treated control to the ROS were calculated. The results are shown in Table 1 below.

Test substance UVB 30 mJ / cm ² Elapsed time after irradiation 0hr 2 hr 3hr Vehicle 100 243 288 UVB + vehicle 100 324 383 UVB + soy extract 100 289 358 UVB + bean leaf extract 100 214 240

As shown in Table 1, it was confirmed that the soybean leaf extract of the present invention inhibits the production of ROS, which is known to cause skin cell damage by ultraviolet light, more effectively than soybean extract. In addition, the extract of canola leaves of the present invention inhibits the amount of ROS after UV stimulation, which is similar to the case of not irradiating ultraviolet rays, indicating that the antioxidative effect is excellent. Therefore, it has been confirmed that the soybean leaf extract according to the present invention can prevent the wrinkling of pores by inhibiting oxidation, preventing aging, and improving the skin troubles by protecting the skin against irritation.

[Test Example 2] Collagenase inhibitory effect

The ability of the soybean leaf extract and soybean extract prepared in Example 1 and Comparative Example 1 to inhibit collagenase production was measured in comparison with tocopherol (Comparative Example 1) and EGCG (Comparative Example 2). Tocopherol and EGCG are substances known to regenerate epidermal cells of the skin and prevent aging of the skin.

Human fibroblasts were plated at 5,000 cells / well in a 96-well microtiter plate containing DMEM (Dulbecco's Modified Eagle's Media) medium containing 2.5% fetal bovine serum, Lt; / RTI > until growth. Then the serum-free one in the DMEM culture medium cultured for 24 hours, and then serum-free the exemplary binary dissolved in DMEM culture medium in Example 1 of kongip extract bean extract of 100ug / ml concentration, comparative example 1 100ug / ml concentration, tocopherol and EGCG, respectively 10 ^{- 4-} molar concentration for 24 hours, and then the cell culture solution was collected.

The degree of collagenase production in the cell culture medium was measured using a collagenase measuring device (Amersham Pharmacia, USA). First, the cell culture solution collected in a 96-well plate uniformly coated with the primary collagenase antibody was added and the antigen-antibody reaction was performed in a thermostatic chamber for 3 hours.

After 3 hours, the second collagen antibody bound to the chromophore was put into a 96-well plate and reacted for another 15 minutes. After 15 minutes, the color development inducing substance was added, coloring was performed at room temperature for 15 minutes, and 1 M sulfuric acid was added to stop the reaction (color development). The color of the reaction solution became yellow, and the degree of yellow was different depending on the progress of the reaction Respectively.

The absorbance of a yellow 96-well plate was measured at 405 nm using a spectrophotometer, and the degree of synthesis of collagenase was calculated by the following equation (1). At this time, the reaction absorbance of the cell culture obtained from the untreated group was used as a control. That is, the degree of expression of collagenase in the untreated group was set at 100, and the degree of expression of collagenase in the group treated with the test substance was determined, and the results are shown in Table 2 below.

[Equation 1]

(%) = Absorbance of the substance-treated cell group / absorbance of the control group X 100

Test substance Expression level of collagenase (%) Untreated group 100 Tocopherol (Comparative Example 1) 76 EGCG (Comparative Example 2) 59 Soybean extract 77 Soybean extract 57

As can be seen from Table 2, the soybean leaf extract inhibited the collagenase more effectively than the soybean extract, and it was confirmed that the tocopherol, which is known as an antioxidant, is superior in inhibiting the expression of collagenase.

[Test Example 3] Effect of increasing cell proliferation

Human skin fibroblasts were inoculated into each well of a 96-well microplate so as to have a skin cell reproduction effect as a skin regeneration effect so as to be 1 × 10 ⁴ cells. And cultured in DMEM (Dulbecco's Modified Eagle's Medium) for 24 hours. After the cultivation, the soybean leaf extract and the soybean extract were replaced with serum-free DMEM medium adjusted to a final concentration of 100 μg / ml, and cultured for another 24 hours. 3- (4,5-Dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium (MTT: 5 mg / ml) was added to each well and allowed to stand for 4 hours, after which the medium was discarded. 100 μl of dimethyl sulfoxide solution was added to each well and the absorbance was measured at 570 nm with a microplate reader.

Tests of the above procedure were conducted to determine the cell proliferation effect from the following equation (2), and the results are shown in Table 3 below.

&Quot; (2) "

Cell proliferation effect (%) = [(absorbance at the time of extract treatment - control absorbance) / control absorbance] X100

Test substance
Concentration (μg / ml) Absorbance (570 nm) Cell proliferation effect (%) 0 (control group) 0.300 - Soybean extract 100 0.383 27.7 Canola Extract 100 0.465 58.0

As shown in Table 3, it was confirmed that the soybean leaf extract proliferated better than the soybean extract.

Test Example 4: Effect of tyrosinase inhibition

The tyrosinase enzyme was extracted from Mushroom and was obtained from Sigma (SIGMA). First, the substrate, tyrosine, is dissolved in distilled water to make a 0.3 mg / ml solution. The solution is put into a test tube in an amount of 1.0 ml. Then, 1.0 ml of potassium phosphate buffer solution (0.1 mol concentration, pH 6.8) and 0.7 ml of distilled water .

0.2 ml of the sample liquid prepared by mixing the soybean leaf extract of the present invention and the soybean extract of Comparative Example 1 in an ethanol solution at a final concentration of 1 mg / ml was added to the reaction solution and reacted for 10 minutes in a 37 ° C thermostatic bath. At this time, the control group was prepared by adding 0.2 ml of a solvent instead of each sample solution, and ascorbic acid was used as a positive control group. 0.1 ml of tyrosinase solution of 2500 units / ml was added to the reaction solution, followed by reaction at 37 ° C for 10 minutes. The reaction tube was immersed in ice water to quench the reaction, and absorbance at a wavelength of 475 nm was measured with a photoelectron spectrometer. The results are shown in Table 2 below. Each tyrosinase inhibitory effect was calculated by the following equation (3).

&Quot; (3) "

Test substance Inhibition rate of tyrosinase (%) Control group (no addition) 0 Ascorbic acid 52 Soybean extract 29 Soybean extract 70

As shown in Table 4, it can be seen that the soybean leaf extract according to the present invention is superior in the inhibition rate of tyrosinase to soybean extract, and is much higher than ascorbic acid, which is a known tyrosinase inhibitor, so that the whitening effect is excellent.

[Test Example 5] Skin moisturizing test

Primary cultured human keratinocytes were placed in a culture flask and adhered to the bottom. Then, the soybean-leaf extract and the soybean extract were added to the culture solution at a concentration of 1 mg / ml, until the cells reached 80 to 90% And cultured for 5 days. The cells were harvested and washed with PBS (phosphate buffered saline). Then, 10 mM Tris-HCl buffer (Tris-HCl, pH 7.4) containing 2% SDS (sodium dodecyl sulfate) and 20 mM DTT (Dithiothreitol) 1 ml was added thereto, sonicated for 3 minutes, and boiled for 10 minutes. Thereafter, it was centrifuged at 1200 rpm for 30 minutes, the precipitate was separated, suspended in 1 ml of PBS, and the absorbance at 340 nm was measured.

Separately, a portion of the solution after the sonication was taken to measure the protein content and used as a criterion when evaluating the degree of cell differentiation. The test results are shown in Table 5 below, in which low calcium (0.03 mM) and high calcium (1.2 mM) treatment groups were used as negative / positive control groups and low calcium concentration test substances were added.

Test substance Concentration used The ability to differentiate in keratinocytes (%) Control group Low Ca ²⁺ (0.03 mM) 100 Control group High Ca ²⁺ (1.2 mM) 206 Soybean extract 1 mg / ml 136 Soybean extract 1 mg / ml 172

As shown in Table 5, when the amount of CE (cornified envelope) produced during keratinocyte differentiation was measured to compare cell differentiation promoting effects, It can be seen that the soybean leaf extract according to the present invention promotes differentiation from keratinocytes in comparison with soybean extract.

[Example 7]

In order to compare the usability of kojic acid, which is a known whitening substance, with the use of an extract of canola leaves used as an active ingredient in the present invention, the degree of irritation such as stinginess and burning was examined in 15 panel members susceptible to stimuli such as stinging and burning .

The subjects were randomly assigned 0.5ml each of kojic acid (purchased from YM chemical) and the luciferase extract obtained in Example 1 at random, and rubbed and rubbed, and the score was scored between 0 and 3.0 in units of 0.1 point. The results are shown in Table 6 below.

<Evaluation Criteria>

0 to 0.4: No stimulation

0.5 to 1.0: slight irritation

1.1 to 2.0: normal irritation

2.1 ~ 3.0: Severe irritation

division Kojic acid Soybean extract Stinginess 0.81 0.21 Burning 0.39 0.19 Average 0.60 0.18

As can be seen in Table 6, in the case of kojic acid, it was found that there was some degree of stinging and burning, and there was a feeling of irritation to the extent that it could feel a slight feeling. The composition of the composition according to the present invention will be described below, but the pharmaceutical composition and the cosmetic composition may be variously applied to various kinds of cosmetic compositions. It is to be understood that the present invention is not limited thereto but is only specifically described.

[Formulation Example 1] Soft capsule

Soft bean capsule was prepared by mixing 150 mg of soybean leaf extract, 2 mg of palm oil, 8 mg of palm kernel oil, 4 mg of yellow radish and 4 mg of lecithin and 400 mg per capsule according to a conventional method.

[Formulation Example 2] Tablets

The granules were prepared by mixing 150 mg of soybean leaf extract, 100 mg of glucose, 50 mg of red ginseng, 96 mg of starch and 4 mg of magnesium stearate and 40 mg of ethanol was added thereto, followed by drying at 60 ° C., Respectively.

[Formulation Example 3] Granules

The granules were formed by mixing 150 mg of soybean leaf extract, 100 mg of glucose, 50 mg of red ginseng extract and 600 mg of starch, and 100 mg of 30% ethanol was added thereto, followed by drying at 60 ° C to form granules. The final weight of the contents was 1 g.

[Formulation Example 4] Drinking agent

150 mg of soybean leaf extract, 10 g of glucose, 50 mg of red ginseng extract, 2 g of citric acid and 187.8 g of purified water were mixed and filled in a bottle. The final volume of the contents was adjusted to 200 ml.

[Formulation Example 5] Preparation of health food

Soybean leaf extract .............................. 1000 mg

Vitamin mixture

Vitamin A Acetate ....................... 70 ㎍

Vitamin E ............................................ 1.0 mg

Vitamin B1 ........................................... 0.13 mg

Vitamin B2 .......................................... 0.15 mg

Vitamin B6 ........................................... 0.5 mg

Vitamin B12 .............................. 0.2 g

Vitamin C ............................................. 10 mg

Biotin ................................................. 10 [mu] g

Nicotinic acid amide .................................. 1.7 mg

Folic acid ................................................. ..... 50 μg

Calcium pantothenate .................................... 0.5 mg

Mineral mixture

Ferrous sulfate .......................................... 1.75 mg

Zinc oxide ............................................ 0.82 mg

Magnesium carbonate ...................................... 25.3 mg

Potassium Phosphate ......................................... 15 mg

Secondary calcium phosphate ...................................... 55 mg

Potassium citrate ............................................ 90 mg

Calcium carbonate .............................................. 100 mg

Magnesium chloride ..................................... 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

[Formulation Example 6] Preparation of health drink

Canned Bean Extract ............................ 1000 mg

Citric acid ................................................. ... 1000 mg

oligosaccharide................................................. .... 100 g

Plum concentrate ................................................ ..... 2 g

Taurine ................................................. ........... 1 g

Purified water was added to the mixture to complete 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was heated for about 1 hour at 85 DEG C with stirring, and the solution thus prepared was filtered to obtain a sterilized 2-liter container, which was sealed and sterilized, &Lt; / RTI &gt;

Although the composition ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the blending ratio according to the regional or national preference such as the demand level, the demanding country, the use purpose, and the like. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

[Formulation Example 1] Lotion

Compounding ingredient weight% Soybean extract 3.00 L-ascorbic acid-2-phosphate magnesium salt 1.00 Water soluble collagen (1% aqueous solution) 1.00 Sodium citrate 0.10 Citric acid 0.05 White pickle extract 0.20 1,3-butylene glycol 3.00 Purified water to 100

[Formulation Example 2] Cream

Compounding ingredient weight% Soybean extract 1.00 Polyethylene glycol monostearate 2.00 Self emulsifying monostearic acid glycerin 5.00 Cetyl alcohol 4.00 Squalene 6.00 Tri-2-ethylhexane glyceryl 6.00 Sphingoglycolipids 1.00 1,3-butylene glycol 7.00 Purified water to 100

[Formulation Example 3] Pack

Compounding ingredient weight% Soybean extract 5.00 Polyvinyl alcohol 13.00 L-ascorbic acid-2-phosphate magnesium salt 1.00 Lauroylhydroxyproline 1.00 Water soluble collagen (1% aqueous solution) 2.00 1,3-butylene glycol 3.00 ethanol 5.00 Purified water to 100

[Formulation Example 4]

Compounding ingredient weight% Soybean extract 2.00 Hydroxyethylene cellulose (2% aqueous solution) 12.00 Xanthan gum (2% aqueous solution) 2.00 1,3-butylene glycol 6.00 Concentrated glycerin 4.00 Sodium hyaluronate (1% aqueous solution) 5.00 Purified water to 100

[Formulation Example 5] Ointment

Compounding ingredient Content (% by weight) Soybean extract 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 Wax 4.0 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 6] ointment

Compounding ingredient Content (% by weight) Soybean extract 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 Wax 4.0 Preservative, coloring, fragrance Suitable amount Purified water Balance

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (7)

A composition for moisturizing comprising an extract of soybean curd as an active ingredient. The composition of claim 1, wherein the composition comprises from 0.001 to 10% by weight of soybean leaf extract, based on the total weight of the composition. The soybean according to claim 1, wherein the soybean is selected from the group consisting of Seoritae, Glycin max MERR, Seomoktae, Rhynchosia Nolubilis , Black soybean, Glycine max (L.) Merr., Blue bean ( Glycime max MERR) , Yellow bean ( Glycime max MERR), field bean ( Vicia faba ), kidney bean ( Phaseolus vulgaris ), pinto bean ( Phaseolus vulgaris L.), small red bean, Vigna angularis Wherein the composition is at least one selected from the group consisting of small black beans ( Phaseolus angularis WF WIGHT.), Sprouting beans ( Glycine max (L.) Merr.) And soy beans (soybean, Glycine max ). 2. The composition of claim 1, wherein the composition promotes differentiation of keratinocytes. 5. The composition according to any one of claims 1 to 4, wherein the composition is a cosmetic composition. 5. The composition according to any one of claims 1 to 4, wherein the composition is a pharmaceutical composition. 5. The composition according to any one of claims 1 to 4, wherein the composition is a health food composition.