KR101882485B1 - Method for measuring liver enzyme and lipid - Google Patents

Abstract

The present invention relates to a measurement method for measuring liver enzymes and lipid contents in the body.
A method for measuring hepatic functional enzyme and lipid content,
A blood sampling step (S100) of collecting a minute amount of capillary blood from the inspector using the sampling means (S); and a step of extracting a minute amount of serum from the capillary blood of the sampled blood sample in the sampling step (S100) using a centrifuge (V) and the lipid-analyzing reagent (P) constituting the serum inlet (I) constituted by the ultra-small amount of serum extracted in the serum extracting step (S200) An information input step S400 for inputting the information of the reader and the inspector through the display unit 120 and the step of injecting the reagent module M of the cartridge type M The serum injected in the serum injecting step S300 is injected into the liver function analyzing reagent V and the lipid analyzing reagent P concentrated through the washing arm 140, And at an appropriate temperature of 37 [deg.] C (S500) for heating the reagent module (M) through the warming part (131) to be heated; and the concentrated hepatic enzyme assay reagent (V) and the lipid analysis reagent (P) after the warming step (S500) A detection step (S600) of detecting the characteristics of the reacted serum using a specific measurement wavelength through a detection unit (150) constituted by a spectrophotometer; And a data storage step (S700) for displaying the detection result detected in the detecting step (S600) on the display unit (120) and storing the detection result in the data storage unit (160) and the mobile memory (U) And the concentration of the functional enzyme and lipid content is measured.
Therefore, the present invention is advantageous in that it provides an accurate result even though it is easy to inspect because the concentrated test reagent (V, P) does not require a lot of capillary blood of the examinee, thereby improving the method of measuring liver function enzyme and lipid content.

Description

[0001] The present invention relates to a method for measuring hepatic functional enzyme and lipid content,

The present invention relates to a method for measuring hepatic functional enzyme and lipid content, and more particularly, to a method for measuring hepatic functional enzyme and lipid content of hepatic functional enzyme < RTI ID = 0.0 > Liver enzymes and lipid contents that provide the reader and the examiner with accurate concentrations of liver enzymes and lipids in the body by measuring the initial rate of reactivity with the test reagent and lipid test reagent and the initial rate of reacting with the specific wavelength of the spectrophotometer And a measurement method.

Generally, in the general hospitals, middle and small hospitals, it is necessary to examine the hepatic enzymatic indicators and lipid indicators by the biochemical test on serum or plasma of the human body, ] And 300 [ul] or more of the test reagent, and the results of the analysis were obtained by a professional operator.

Therefore, there is a need for a measurement method that can be linked to the miniaturization of the measuring apparatus while maintaining the accuracy of the detection result while reducing the blood sampling amount of the inspector.

As a prior art relating to a measurement method for measuring the liver enzymes and lipid contents in the body, as shown in FIG. 8 (a), the "liver function test method" (hereinafter referred to as " Technique 1 ") comprises: introducing a blood sample passing through a liver of a patient and a bile acid derivative of an effective amount of colored or fluorescent into a patient's vein, treating the collected blood sample every predetermined time, The blood sample is treated so as to obtain a plasma, the color or fluorescence of the bile acid derivative is measured in the plasma, the measured value obtained in the step of measuring the color or fluorescence of the bile acid derivative in the plasma is adjusted to the plasma reduction curve, By comparing the plasma reduction curve obtained in the subject with the plasma reduction curve obtained above to determine the patient ' s liver function And evaluating the color or fluorescence of the bile acid derivative in each sample.

As another prior art, as shown in FIG. 8 (b), a method of quickly analyzing and examining cholesterol and triglycerides with a small amount of sample of Korean Patent Laid-Open Publication No. 10-2015-0094710 (hereinafter referred to as " Prior Art 2 ') is a reagent, assay, kit, device and system for rapidly measuring cholesterol and cholesterol fractions from a blood sample. Total cholesterol, low density lipoprotein cholesterol and high density lipoprotein cholesterol are cholesterol variants can be measured by a single analytical test using kinetic measurements under conditions that convert sub-species into detectable results at a definable (rate) rate, After initiation, measurements were made at different time points to detect lipolytic enzymes, cholesterol esterase, cholesterol oxidase and peroxidase A method for calculating a hypercholesterolemic lipoprotein cholesterol level, including the measurement of triglycerides, which can produce colored results in direct proportion to the amount of converted cholesterol used together, provides analytical tests with a single reaction mixture So that the cholesterol values can be determined more precisely and accurately at a lower cost than can be obtained by carrying out a number of different analytical tests with various reaction mixtures and the ratio of cholesterol sub-fraction to total cholesterol can be determined .

However, the method of the prior art 1 requires a large amount of blood collection from an examiner as in the prior art, and Prior Art 2 is a method of intensively analyzing body cholesterol as a single mixture analysis test.

Korean Patent Registration No. 10-0444564 (Aug. 2004) Korean Patent Publication No. 10-2015-0094710 (2015.08.19)

SUMMARY OF THE INVENTION Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art,

Conventionally, in order to measure the hepatic functional enzyme and lipid content in the body, a large amount of blood sampling and expensive measuring equipment were required from the examiner. However, since the present invention uses a concentrated test reagent, only a minute amount of capillary blood can be used to measure the liver enzymatic function and lipid content The present invention is directed to a method for measuring a sample.

Another object of the present invention is to isolate serum from an extremely small amount of capillary blood during the examination so that the reader and the examiner can reduce the burden of blood sampling, thereby not only psychological stability in blood drawing but also separation from ultrafine capillary blood The objective of the present invention is to maintain and improve the accuracy of the detection results because it is possible to accurately determine the objective liver enzymes and lipid contents by the concentrated test reagents even if the serum is used.

In addition, since the concentrated test reagent and a very small amount of serum are used, the volume of the measuring device used by the measuring method of the present invention can be reduced, and the portable and easy to manage the measuring device can be easily used.

In order to accomplish the above object, the present invention has been made to solve the above problems,

A method for measuring liver enzymes and lipid contents,

A blood collecting step of collecting a minute amount of capillary blood from an inspector using a collecting means;

A serum extracting step of extracting a trace amount of serum from a capillary blood of a blood sampler collected in the blood collecting step using a centrifuge;

A serum injecting step of injecting the ultra-minute amount of serum extracted in the serum extracting step into a cartridge type reagent module in which a serum injection port is constituted and a concentrated hepatic enzyme assay reagent and a lipid assay reagent are stored;

An information input step of inputting information of a reader and an inspector through a display unit;

The reagent module is inserted into the liver function enzyme and lipid content measuring device, and the serum injected in the serum injection step is transferred to react with the hepatic enzyme assay reagent and the lipid assay reagent concentrated through the washing arm, A heating step of heating the reagent module through a heating part to be reacted;

A detection step of detecting a result of the serum reacted with the liver enzyme assay reagent and the lipid assay reagent concentrated after the warming step using a specific measurement wavelength through a detection unit constituted by a spectrophotometer;

And a data storage step of displaying the detection result detected in the detection step on a display unit and storing the detected result in a data storage unit and a mobile memory, wherein the concentration of liver enzymes and lipid content in the body of the examinee is measured.

At this time, in the detecting step,

AST, ALT and GGT, which are the test items of hepatic enzymes, are measured by KINETIC TEST at 340 nm, 340 nm and 405 nm, respectively. and,

The lipid test items TCHO, TG, HDL-C and LDL-C are the endpoints, and the result is calculated as the magnitude of the absorbance according to the concentration of the serum at a measurement wavelength of 546 [nm] Is configured to be disposable.

It should be understood, however, that the terminology used herein is for the purpose of describing the claimed invention in its broadest possible form and should not be construed in a limiting sense. It should be construed as meaning and concept consistent with the technical idea of the present invention.

Therefore, the embodiments described in the present specification and the configurations shown in the drawings are merely the most preferred embodiments of the present invention, and not all of the technical ideas of the present invention are described. Therefore, various equivalents It should be understood that water and variations may be present.

As described above, according to the present invention,

Conventionally, in the measurement of liver enzymes and lipids contents in the body, a large amount of blood sampling and expensive measuring equipment were required from the examiner. However, since the present invention is capable of measuring liver enzymatic activity and lipid content with a very small amount of serum due to concentrated test reagents Inspection is easy.

In addition, since a very small amount of serum is required, administrators and inspectors can reduce the burden of collecting blood, thereby not only psychologically stabilizing the test, but also using an ultra-small amount of serum, And the lipid content can be accurately determined.

In addition, the present invention provides a concentrated assay reagent for measuring a specific detection of a trace amount of serum and liver function enzymes (AST, ALT, GGT) and lipid (TCHO, TG, HDL-C, LDL- It is possible to use a measuring device having a small volume corresponding thereto, which is a very effective invention for facilitating the carrying and management of the measuring device.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a block diagram illustrating a measurement method for measuring liver enzymes and lipid contents in the body of the present invention. FIG.
FIG. 2 is a perspective view showing an embodiment of a liver function enzyme and a lipid content measuring instrument used in a measurement method for measuring the content of liver enzymes and lipids in the body of the present invention. FIG.
FIG. 3 shows an example of a measurement flow chart using a liver function enzyme and a lipid content measuring instrument.
FIG. 4 is a table showing the amounts of raw materials of the hepatic enzyme-assay reagent concentrated in the reagent module among the measurement methods for measuring the contents of liver enzymes and lipids in the body of the present invention.
FIG. 5 is a table showing the amounts of raw materials of the lipid assay reagent concentrated in the reagent module among the measurement methods for measuring the content of liver enzymes and lipids in the body of the present invention.
FIG. 6 is a table showing the performance of the test reagent concentrated in the reagent module among the measurement methods for measuring the content of liver enzymes and lipids in the body of the present invention.
FIG. 7 is a table summarizing terms of raw materials of the test reagent concentrated in the reagent module among the measurement methods for measuring the content of liver enzymes and lipids in the body of the present invention.
FIG. 8 shows a representative diagram of the prior art for the measurement method for measuring the content of liver enzymes and lipids in the body of the present invention.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the constitution and operation of a measuring method for measuring the content of liver enzymes and lipids in the body of the present invention will be described in detail with reference to the accompanying drawings.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a block diagram illustrating a measurement method for measuring liver enzymes and lipid contents in the body of the present invention. FIG.

A method for measuring liver enzymes and lipid contents,

A blood collection step (S100) of collecting a minute amount of capillary blood from the examiner using the blood collecting means (S);

A serum extraction step (S200) of extracting a trace amount of serum from the capillary blood of the blood sampler collected in the blood collection step (S100) using a centrifugal separator;

The ultrafiltration serum extracted in the serum extraction step (S200) is transferred to a reagent module (cartridge type) in which the serum injection port (I) is formed and the concentrated hepatic enzyme assay reagent (V) and lipid assay reagent (P) M) (S300);

An information input step (S400) of inputting information of a reader and an inspector through the display unit 120;

The reagent module M is inserted into the liver function enzyme and lipid content measuring instrument 100 and the serum injected in the serum injecting step S300 is injected into the blood circulation system through the washing arm 140, A heating step (S500) of heating the reagent module (M) through the heating part (131) to be reacted with the inspection reagent (P) and reacting at an appropriate temperature of 37 [캜];

The characteristics of the serum reacted with the hepatic enzyme assay reagent (V) and the lipid assay reagent (P) after the warming step (S500) are measured using a specific measurement wavelength through the detection unit 150 constituted by the spectrophotometer, (S600);

And a data storage step S700 of displaying the detection result detected in the detecting step S600 on the display unit 120 and storing the detection result in the data storage unit 160 and the mobile memory U, And the concentration of enzyme and lipid content is measured.

At this time, in the detecting step S600,

AST, ALT and GGT, which are the test items of hepatic enzymes, are measured by KINETIC TEST at 340 nm, 340 nm and 405 nm, respectively. and,

The lipid test items TCHO, TG, HDL-C and LDL-C are end-points, which are calculated as the magnitude of the absorbance according to the concentration of serum at a measurement wavelength of 546 [nm].

AST, ALT and GGT among the test items of liver function enzymes are detected by using a spectrophotometer at 340 nm measurement wavelength step (S610) and 405 nm measurement wavelength measurement step (S620), respectively, and lipid test items TCHO, TG, HDL-C, and LDL-C are detected using a spectrophotometer at the 546 nm measurement wavelength measuring step (S630).

FIG. 4 and FIG. 5 are tables showing the amounts of raw materials of hepatic enzyme-assay reagent and lipid-analyzing reagent concentrated in a reagent module among the measurement methods for measuring the content of liver function enzymes and lipids in the present invention. Specifically,

AST catalyzes the transamination of the amino acids of el-aspartic acid and alpha-ketoglutarate to produce glutamate and oxaloacetate, which are abundant in the myocardium, liver, brain and other tissues, and are thus used in the diagnosis and treatment of liver and heart disease.

The principle of measurement is that when serum and plasma react with the substrate enzyme solution (R1, R2) enriched in the reagent module (M), glutamic acid and oxaloacetate are produced by catalytic reaction of AST, and oxaloacetate is reacted with malate dehydrogenase At the same time, NADH is oxidized to NAD.

Therefore, the activity of AST in serum and plasma is determined by measuring the decrease in absorbance at a measurement wavelength of 340 [nm] by oxidation of NADH.

The concentrated test reagent of the present invention for testing AST is divided into an enzyme reagent (R1) and a substrate reagent (R2)

The enzymatic reagent R1 contained 0.25 mg of Tris (TRISMA BASE) buffer, 4.6 mg of L-Aspartic acid, 0.14 ku of Malate dehydrogenase, 0.37 ku of LDH (lactate dehydrogenase) ],

The substrate reagent R2 is composed of 0.25 mg of Tris (TRISMA BASE) buffer, 0.006 mg of NADH (β-Nicotinamide adenine dinucleotide), and 0.62 mg of alpha-ketoglutarate.

ALT catalyzes the transamination of L-alanine and alpha-ketoglutarate to produce glutamic acid and pyruvic acid, which are abundant in the liver, kidney and other tissues, and are thus used in the diagnosis and treatment of liver disease.

The principle of measurement is that when serum and plasma are reacted with substrate enzyme solutions (R1, R2) enriched in reagent module (M), glutamic acid and pyruvic acid are produced by catalytic reaction of ALT, and pyruvic acid is converted to lactic acid by lactic dehydrogenase NADH is oxidized to NAD.

Therefore, the activity of ALT in serum and plasma is determined by measuring the decrease in absorbance at a measurement wavelength of 340 [nm] by oxidation of NADH.

The concentrated test reagent of the present invention for testing ALT is divided into an enzyme reagent (R1) and a substrate reagent (R2)

The enzyme reagent R1 is concentrated to 0.25 mg of Tris (hydroxymethyl) amino methane, 4.76 mg of L-Alanine and 0.4342 ku of Latate dehydrogenase,

The substrate reagent R2 is composed of 0.24 mg of Tris (hydroxymethyl) amino methane, 0.0661 mg of NADH and 0.6136 mg of α-ketoglutaric acid disodium salt.

Γ-GTP of GGT is an enzyme that degrades gamma-glutamyl peptides into other peptides or amino acids that are abundant in the kidney, pancreas, liver or other tissues due to the activity of gamma-glutamylase. Significant clinical biochemical parameters are considered to be important parameters in observing alcoholic liver disease and its treatment trends.

The principle of measurement is that the serum and plasma react with the buffer solution (R1) and the substrate reagent (R2) enriched in the reagent module (M) to form the γ-glutamyl group of the L-γ-glutamyl- glutamylglycylglycine and 5-amino-2-benzoic acid are liberated as a result of the action of γ-GTP, and the ratio of the 5-amino-2-benzoic acid is measured at a measurement wavelength of 405 [nm] And the activity of γ-GTP in serum and plasma is measured.

The concentrated test reagent of the present invention for inspecting GGT is divided into a buffer solution (R1) and a substrate reagent (R2)

The buffer (R1) is concentrated to 1.11 [mg] in glycylglycine buffer (pH 8.14)

The substrate reagent (R2) is constituted by concentrating L-gamma -glutamyl-3-carboxy-4-nitroaniline to 0.64 [mg].

The principle of measurement of TCHO is that when serum and plasma are added to enzyme reagent (R1) and substrate reagent (R2) concentrated in reagent module (M), cholesterol ester in serum and plasma becomes hydrolyzed to become cholesterol and cholesterol esterase Cholesterol is already produced in serum and plasma and is oxidized by CO (carbon monoxide) to produce hydrogen peroxide.

Hydrogen peroxide is quantitatively oxidized and condensed by 4 - amino - antipyrine, TOOS and POD enzymes, and the amount of total cholesterol in serum and plasma is determined by absorbance measurement at a measuring wavelength of 546 [nm].

The concentrated test reagent of the present invention for testing TCHO is divided into an enzyme reagent (R1) and a substrate reagent (R2)

The enzyme reagent R1 was concentrated to HEPES 1.192 [mg], POD 0.1 [ku], CHE 0.09 [ku] and ASOD 0.182 [ku]

The substrate reagent R2 is composed of HEPES 1.192 [mg], COD 0.24 [ku], and TOOS 0.064 [mg].

TG is a lipid of serum composed of triglyceride (triglyceride), cholesterol, phospholipid, free fatty acid and trace amount of fat-soluble substance such as fat soluble vitamin and carotene.

The measurement of triglyceride in serum is an important test for the diagnosis of arteriosclerosis, coronary artery disease and diabetes. The principle of measurement is that triglyceride in serum is decomposed into glycerol and fatty acid by the action of lipoprotein lipase (LPL). Glycerolkinase GK) and ATP act on α-Glycerol-3-phosphate and ADP.

At this time, α-Glycerol-3-phosphate is α-Glycerol-3-phosphate- oxidase (GPO) of H ₂ O ₂ generating a H ₂ O ₂ by the action and the generation of the 4-Aminoantipyrine presence of Peroxidase (POD) (4-AAP) and ADPS are measured by colorimetry at a measurement wavelength of 546 [nm].

The concentrated test reagent of the present invention for testing TG is divided into an enzyme reagent (R1) and an enzyme reagent (R2)

The enzyme reagent R1 contained PIPES 1.512 [mg], glycerol kinase (GK) 0.3 [ku], ATP 0.109 [mg], GPO 0.8 [ku], ascorbic acid oxidase 0.101 [ku], 4-aminoantipyrine 0.0106 [mg ],

The enzyme reagent R2 is composed of PIPES 1.512 [mg], peroxidase (POD) 0.272 [ku], and lipoprotein lipase (LPL) 80 [ku].

It has been reported that serum total cholesterol levels have been associated with coronary heart disease for a long time. Recently, it has been reported that the HDL-C level and the incidence of coronary heart disease are closely related, so that high density lipoprotein cholesterol ) Is a risk factor for the development of coronary heart disease and is an important test for diagnosing this disease.

Although selective chemical precipitation has been widely used to quantify HDL-C, this method is not suitable for large-scale analytical laboratories due to the separate precipitation procedure.

Therefore, the present invention does not require a precipitation operation using a specific surfactant as an assay reagent for quantitative inspection, and can be applied to various automatic analyzers.

Cholesterol, LDL, cholesterol and cholesterol are produced by CHE, COD and POD enzymes under specific conditions. Cholestenone and H ₂ O ₂ are produced by the enzymes of Chylomicron, VLDL and LDL in the first step. H ₂ O ₂ is decomposed into water and oxygen by catalase.

The remaining HDL-Cholesterol in step 2 forms a pigment by CHE, COD and POD enzymatic reaction in the presence of a specific surfactant. The change of absorbance of this dye is measured and the concentration of HDL-Cholesterol is measured at a measurement wavelength of 546 [nm] .

The concentrated test reagent of the present invention for testing HDL-C is divided into an enzyme reagent (R1) and an enzyme reagent (R2)

The enzyme reagent R1 is concentrated to HEPES 1.192 [mg], POD 0.1 [ku], ASOD 0.182 [ku], COD 0.08 [ku] and DSBmT 0.064 [mg]

The enzyme reagent R2 is composed of HEPES 1.192 [mg], CHE 0.27 [ku], and 4-aminoantipyrine 0.0342 [mg].

In the case of LDL-C, blood total cholesterol is closely related to cardiovascular disease, and it is an important test to assess the blood level of LDL-C and the risk of developing coronary vascular disease.

Recently, it has been tested by beta-quantification method which is a combination of ultracentrifugation and chemical precipitation. However, this method is not suitable for general examination because it has many manual and high machine dependence.

Therefore, the Friedewald method has been used as the most general LDL calculation method. However, since this calculation method calculated from total cholesterol depends on the precision and accuracy of TG and HDL-C, it is impossible to measure pure LDL concentration and TG is 250 [mg / And it has limitations in that it can be used only in the fasting state.

Therefore, in the present invention, the LDL-C value is calculated from the equation according to the analysis of TG and TCHO by the measurement principle.

In this case,

LDL-C = TOTAL CHOLESTEROL - HDL CHOLESTEROL - TG / 5, and the unit is [mg / dL].

FIG. 2 is a perspective view showing an embodiment of a liver function enzyme and a lipid content measuring instrument used in a measurement method for measuring the content of liver enzymes and lipids in the body of the present invention. FIG.

As shown in FIG. 2, the liver function enzyme and lipid content measuring device 100 may be configured to measure the content of the liver function enzyme,

A reagent module (M) of a cartridge type in which a serum injection port (I) is constituted and in which concentrated hepatic enzyme assay reagent (V) and lipid assay reagent (P) are stored;

A housing 110 having a predetermined shape;

A display unit 120 located outside the front surface of the housing 110 for displaying information of a reader and an inspector inputting information, detection process guidance, and detection result information;

When the reagent module inserting portion button B is pressed, the reagent module inserting portion B is automatically protruded to be able to insert the reagent module M, and when the reagent module inserting portion button B is pressed again A reagent module inserting part 130 which is returned to the inside of the housing 110;

The reagent module (M) inserted into the housing (110) is heated so as to be reacted at a suitable temperature of 37 [캜] by reacting serum and concentrated hepatic enzyme assay reagent (V) and lipid assay reagent (P) A heating part 131 formed in the reagent module inserting part 130;

A washing arm 140 for transferring the serum so as to react with the hepatic enzyme assay reagent (V) and the lipid assay reagent (P), the serum of which is concentrated;

A detector 150 for optically measuring the result of the reaction of serum and concentrated hepatic enzyme assay reagent (V) and lipid assay reagent (P) with a spectrophotometer to detect the liver liver function enzyme and lipid content of the examinee;

A data storage unit 160 for storing information of a reader and an inspector and a result of the detection unit 800;

A communication unit 170 capable of transmitting and receiving data of the data storage unit 160 to the personal terminal H;

A port 180 coupled with the portable memory U to move or store data stored in the data storage unit 160 using the portable memory U;

The controller 120 controls the display unit 120, the reagent module inserting unit 130, the warming unit 131, the washing arm 140, the detecting unit 150, the data storing unit 160, the communication unit 170, A control unit 190 for controlling the control unit 190;

And a power unit 200 located at the lower end of the display unit 120 to supply or cut off power.

FIG. 3 shows an example of a measurement flow chart using the liver function enzyme and lipid content measuring device 100,

3, the power source unit 200 is operated to supply power (power supply step, S10), and information of the reader and the inspector is input through the display unit 120 (inserter information input step, S20) The reagent module inserting portion 130 is protruded from the housing 110 when the reagent module inserting portion button B is pressed.

Then, the reagent module M injected with the serum extracted from the capillary blood of the inspector is inserted into the protruded reagent module inserting section 130 (the reagent module inserting step, S30).

The reagent module inserting portion 130 protruding from the housing 110 in which the reagent module M is inserted is artificially pressed in the horizontal direction for a predetermined period of time or the reagent module inserting portion button B is pressed again, (130) can be positioned inside the housing (110).

At this time, it is confirmed whether the reagent module M is inserted into the reagent module inserting part 130 inserted into the housing 110 through the control part 190 (insertion inspecting step, S40), and detected by the detecting part 150 (Detection step, S50) is performed.

The detection step S50 may be performed in such a manner that the serum of the examinee injected into the reagent module M is supplied to the reagent module M and the reagent V and the lipid (Reaction reagent reaction step, S51) with the reagent (TCHO, TG, HDL-C, LDL-C)

In the test reagent reaction step (S51), the temperature is controlled by the warming part (131) so that the serum and the concentrated test reagents (V, P) can be reacted at an appropriate temperature of 37 [ AST and ALT were measured at 340 nm and GGT at 405 nm, respectively. The TGO, TG, and TG values were calculated by the KINETIC TEST, HDL-C and LDL-C are endpoints, and the result is calculated as the magnitude of the absorbance according to the concentration of serum at a measurement wavelength of 546 [nm] (spectrophotometer measurement step S52).

In this detection step S50, information on the detection process guidance can be displayed through the display unit 120 so that the reader and the inspector can check the detection process in real time. The detection reagent reaction step (S51), the spectrophotometer measurement (Detection result output step, S60) of the detection result detected in the detection step S50 including step S52.

The detection result is stored (data storage step, S80) in the data storage unit 160 according to the decision of the administrator and the inspector, and the measurement is terminated (end step S1 )do.

In addition, the cartridge type reagent module M composed of the concentrated hepatic enzyme assay reagent (V) and the lipid assay reagent (P) is configured to be disposable so that the assay can be easily performed.

FIG. 6 is a table showing the performance of the test reagent concentrated in the reagent module among the measurement methods for measuring the content of liver enzymes and lipids in the body of the present invention. FIG. 7 is a graph showing the results of measurement Among the measurement methods, the table lists the terms of raw materials of the test reagent concentrated in the reagent module.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention as defined in the appended claims.

Accordingly, the invention may be embodied in many other forms without departing from the spirit or essential characteristics thereof, and therefore, the embodiments of the present invention are to be considered in all respects as illustrative and not in a limiting sense.

The present invention relates to a method for detecting hepatic enzymatic activity and lipid content by detecting hepatic enzymatic activity and lipid content by reacting serum extracted from ultrafine capillary blood with concentrated hepatic enzymes and lipid assay reagents, And the lipid content can be easily measured, so that the present invention can be applied to contribute to the promotion of various industrial fields such as medical business that maintains health by periodically managing the detection result of the inspector.

S100: Blood collection step S200: Serum extraction step
S300: serum injection step S400: information input step
S500: heating step S600: detection step
S610: 340 nm measurement wavelength measurement step
S620: 405 nm measurement wavelength measurement step
S630: 546 nm measurement wavelength measurement step
S700: Data storage step
100: liver function enzyme and lipid content meter
110: housing 120: display part
130: Reagent module insertion part 131:
140: washing arm part 150: detecting part
160: Data storage unit 170: Communication unit
180: port 190:
200:
B: cartridge insertion portion button I: serum injection port
M: Reagent module P: Concentrated lipid test reagent
S: Blood collection means U: Movable memory
V: Enriched hepatic enzyme assay reagent
S10: Power supply step S20: Tester information input step
S30: Reagent module insertion step S40: Insertion confirmation step
S50: detection step S51: test reagent reaction step
S52: Spectrophotometer measurement step S60: Detection result output step
S70: Detection result storing step S80: Data storing step
S90: additional detection step S1: end step

Claims (3)

A cartridge type reagent module M storing a concentrated hepatic enzyme assay reagent V and a lipid assay reagent P constituted by a serum injection port I, a housing 110 having a predetermined shape, A display unit 120 located outside the front surface of the housing 110 for displaying information on the input of a reader and an examiner and guidance of detection procedures and detection result information; A reagent module inserting part 130 which is automatically protruded so as to insert the reagent module M when pressed, and which is returned to the inside of the housing 110 when the reagent module inserting part button B is pressed again, A reagent module insertion step of heating the reagent module M inserted into the housing 110 so as to react at a proper temperature of 37 [캜] by reacting the hepatic enzyme assay reagent (V) and the lipid assay reagent (P) A heating part 131 formed in the part 130, A washing arm 140 for transferring the serum so as to react with the aggravated liver enzyme assay reagent V and the lipid assay reagent P and the serum and concentrated hepatic enzyme assay reagent V and the lipid analysis reagent P, A data storage unit 160 for storing the information of the reader and the examiner and the results of the detector 800, A communication unit 170 that can transmit and receive data of the data storage unit 160 to and from the personal terminal H and a portable memory unit 170 that can move or store data stored in the data storage unit 160 using the portable memory U, A display unit 120, a reagent module inserting unit 130, a warming unit 131, a washing arm 140, a detecting unit 150, a data storing unit 160, A communication unit 170, a control unit 190 for controlling the port 180, a display unit 120, Located at the bottom through the enzyme function and lipid content measuring devices (100) consisting of inter-power supply unit (200) to supply or cut off the power,
From the examiner, using the blood-collecting means (S), 0.5 to 5.0 [mu] A blood sampling step (S100) for collecting a minute amount of capillary blood, a serum extracting step (S200) for extracting a minute amount of serum from the capillary blood of the blood sampler collected in the blood collecting step (S100) The ultrafiltration serum extracted in step S200 is transferred to a cartridge containing the liver function assay reagent (V) and lipid assay reagent (P) which are constituted by the serum injection port (I) and are concentrated to 10 to 30 [ (S300) for injecting the reagent module (M) in the form of a reagent; a step (S400) for inputting information of the reader and the inspector through the display unit (120) The serum injected in the lipid content measuring device 100 and injected in the serum injecting step S300 is transported through the washing arm 140 to react with the concentrated hepatic enzyme assay reagent V and the lipid assay reagent P, Through the warming portion 131 so as to be reacted at an appropriate temperature of 37 [ (S500) for heating the weak module (M), the characteristics of the serum reacted with the hepatic enzyme assay reagent (V) and the lipid assay reagent (P) concentrated after the warming step (S500) A detection step S600 including a 340 nm measurement wavelength measurement step S610, a 405 nm measurement wavelength measurement step S620, and a 546 nm measurement wavelength measurement step S630 for detecting a result using a specific measurement wavelength through the detection unit 150, And a data storing step (S700) of displaying the detection result detected in the detecting step (S600) on the display unit (120) and storing the data in the data storing unit (160) and the mobile memory (U) And lipid content,
A method for measuring hepatic functional enzyme and lipid content,
In the detecting step S600,
AST, ALT and GGT, which are the test items of hepatic enzymes, are measured by KINETIC TEST at 340 nm, 340 nm and 405 nm, respectively. and,
The endotoxicity of TCHO, TG, HDL-C and LDL-C was measured at 546 [nm] as the end point,
Based on the calculated values of TCHO, TG and HDL-C, LDL-
A method for measuring hepatic functional enzyme and lipid content in a body, which is characterized by being calculated by a formula of LDL-C [mg / dL] = TOTAL CHOLESTEROL-HDL CHOLESTEROL-TG / 5. delete delete

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