KR101810017B1

KR101810017B1 - Container for nucleic acid amplification reaction

Info

Publication number: KR101810017B1
Application number: KR1020147002886A
Authority: KR
Inventors: 청 쑤; 핑화 텅
Original assignee: 제네리치 바이오테크놀로지 코포레이션
Priority date: 2011-07-12
Filing date: 2011-07-12
Publication date: 2017-12-18
Also published as: EP2733198B1; CN103635569A; KR20140034918A; CA2841019A1; EP2733198A4; EP2733198A1; WO2013007021A1; CN103635569B; CA2841019C

Abstract

The present invention includes a capillary tube 100 and a thermally conductive sleeve 200 which is mounted on the outside of the capillary tube 100 so that when the thermal energy is conducted to the thermally conductive sleeve, And the capillary tube 100 receives heat uniformly to thereby enhance the reaction rate of the nucleic acid amplification reaction.

Description

CONTAINER FOR NUCLEIC ACID AMPLIFICATION REACTION [0002]

The present invention relates to a nucleic acid amplification reaction, and more particularly to a nucleic acid amplification reaction container.

The nucleic acid amplification reaction refers to a technique of repeatedly using the same manipulation procedure and amplifying a nucleic acid by binding a specified polymerase. The most common polymerase chain reaction (PCR), reverse transcription polymerase chain reaction (RT-PCR), real-time polymerase chain reaction (PCR) ) Belong to the nucleic acid amplification reaction technique.

Polymerase chain reaction refers to a technique for amplifying a specific DNA fragment. The term "reverse transcription polymerase chain reaction" refers to a technique in which DNA is obtained using mRNA transcription and then the above-described polymerase chain reaction is performed using the DNA. Real-time polymerase chain reaction refers to a technique for performing semi-quantitative test using a fluorescent probe or dye in the course of a polymerase chain reaction, and is also referred to as quantitative PCR. All of the techniques described above require the use of polymerase chain reaction techniques.

Relatively new technologies such as Rolling Circle Amplification (RCA), Loop Mediated Isothermal Amplification (LAMP), Nucleic Acid Sequence Based Amplification (NASBA), and TWJ (Three Way Junction) also require the use of polymerase chain reaction technology.

In the polymerase chain reaction, DNA and primers are mixed in a buffer solution, and double strands of DNA are separated using a temperature of about 90 캜; Then, using a temperature of about 50 캜, the primer is attached to a specific position of the DNA, and then, A temperature of about 70 < 0 > C is used to extend the primer attached to the DNA. Repeat this procedure to duplicate a specific DNA fragment.

At present, there are many types of apparatuses used to carry out the above-described heating process. Among them, a comparatively inexpensive type is a heating device installed at both ends of a container (usually a test tube), in which one heating device is fixed to heat up to 90 ° C and the other heating device is heated to 50 ° C Fixed. Convection occurs in the solution in the container due to the temperature difference, and the polymerase chain reaction is carried out by allowing the DNA and the primer in the solution to circulate between 90 ° C and 50 ° C.

However, the conventional heating apparatus is usually a metal block, and the upper portion has a concave groove for placing the container, and the shape of the concave groove is matched with the container. By placing the container in a heating device and raising the temperature of the heating device to a suitable temperature, the container can be heated. A disadvantage of this type of heating device is that in actual manufacture the recessed groove can not be perfectly matched to the container. In other words, the recessed groove may be protruded or recessed. The protruding portion prevents the peripheral portion from contacting the container, and the depressed portion makes it impossible to contact the container at the depressed portion. In this case, the container does not receive the heat uniformly and affects the reaction rate of the polymerase chain reaction, that is, the nucleic acid amplification reaction.
[Prior art] US 6,068,978 B2

It is a technical object of the present invention to provide a container for a nucleic acid amplification reaction in which the container is uniformly heated using closely fitting techniques.

According to one embodiment of the present invention, the container for nucleic acid amplification reaction includes a capillary tube and a thermally conductive sleeve. The thermally conductive sleeve is mounted on the outside of the capillary to uniformly provide thermal energy to the capillary.

According to the container for nucleic acid amplification reaction of the present invention, the thermally conductive sleeve is a fuselage.

According to the container for nucleic acid amplification reaction of the present invention, the thermally conductive sleeve is a fastener.

According to the container for nucleic acid amplification reaction of the present invention, the thermally conductive sleeve is of the C type.

According to the container for nucleic acid amplification reaction of the present invention, the material of the capillary is plastic.

According to the container for nucleic acid amplification reaction of the present invention, the material of the capillary is polycarbonate.

According to the container for nucleic acid amplification reaction of the present invention, the material of the heat-conducting sleeve is metal.

According to the container for nucleic acid amplification reaction of the present invention, the material of the heat conductive sleeve is iron.

According to the container for nucleic acid amplification reaction of the present invention, the capillary includes an annular groove, and the annular groove accommodates and fixes the heat conductive sleeve.

According to the present invention, when the thermal energy is conducted to the thermally conductive sleeve, the capillary can be uniformly heated as compared with the prior art.

1 is a perspective view illustrating a container according to an embodiment of the present invention.
2 is an exploded view of the container of Fig.
3 is a cross-sectional view taken along line AA in Fig.

1 is a perspective view illustrating a container according to an embodiment of the present invention. 2 is an exploded view of the container of Fig. As shown in the figure, the container includes a capillary tube 100 and a heat-conducting sleeve 200. The heat conducting sleeve 200 is mounted on the outside of the capillary tube 100.

3 is a sectional view taken along the line A-A in Fig. The thermally conductive sleeve 200 described above is mounted on one end 110 of the capillary 100, and the one end 110 is a closed end. The capillary 100 is accommodated in the concave groove 310 of the heat source 300 and heats the heat conductive sleeve 200 using the heat source 300 so that the heat generated by the heat source 300 Energy can be used to heat one end 110 of the capillary 100. The temperature of the one end 110 of the capillary tube 100 is controlled to about 90 ° C and the temperature of the other end is lowered to about 50 ° C by using the ambient temperature so that the nucleic acid amplification reaction can be performed in the capillary tube 100.

Since the thermal conductive sleeve 200 is mounted on the outer side of the capillary tube 100, thermal energy of the thermal conductive sleeve 200 can be uniformly conducted to the capillary tube 100. Also, since the capillary tube 100 does not directly contact with the heat source 300, a situation in which heat is not received uniformly does not occur. The heat conduction sleeve 200 can be used to uniformly receive the heat from the capillary tube 100, thereby improving the reaction speed of the nucleic acid amplification reaction.

Referring to FIG. 2, the capillary 100 may include an annular groove 120. The annular groove 120 is located at one end 110 of the capillary tube 100 to receive and position the heat conductive sleeve 200.

The thermally conductive sleeve 200 may be an annulus and the inner diameter of the thermally conductive sleeve 200 may be matched with the outer diameter of the capillary tube 100 to allow the thermally conductive sleeve 200 to be mounted outside the capillary tube 100. The inner diameter of the thermal conductive sleeve 200 is smaller than or equal to the outer diameter of the capillary tube 100 and the thermal conductive sleeve 200 is deformed and the outer surface of the capillary tube 100 is deformed, As shown in FIG. More specifically, when the heat conductive sleeve 200 is a fastener, the shape of the heat conductive sleeve 200 may be C-shaped. That is, the heat conductive sleeve 200 may be a C-type fastener.

In the technique of mounting the thermally conductive sleeve 200 on the capillary tube 100, when the thermally conductive sleeve 200 is an annulus, the inside of the thermally conductive sleeve 200 is completely in contact with the outside of the capillary tube 100. When the thermally conductive sleeve 200 is a fastener, the inside of the heat conductive sleeve 200 is completely in contact with the outside of the capillary tube 100.

The material of the capillary tube 100 is plastic, and furthermore, the material of the capillary tube 100 is polycarbonate (PC). The material of the heat conductive sleeve 200 is metal, and furthermore, the material of the heat conductive sleeve 200 is iron. The above-described materials of the capillary tube 100 and the heat-conducting sleeve 200 are compared with each other according to conditions such as heat-resistant temperature and strength, so that the cost of the product can be lowered because the material is inexpensive and comparatively inexpensive.

100: capillary tube
200: Heat conductive sleeve
110: one end of the capillary
300: heat source

Claims

A capillary (100) comprising a closed end; And
A thermal conductive sleeve 200 mounted on the outer side of the capillary tube 100 and fitted in the closed end 110 of the capillary tube,
Lt; / RTI >
In the heat conductive sleeve 200, a portion of the capillary tube 100 excluding the closed end 110 is exposed to the outside,
The other end of the capillary tube 100 contacts the ambient temperature and the height of the liquid level of the solution in the capillary tube 100 is higher than the upper end of the thermally conductive sleeve 200,
Containers for nucleic acid amplification reactions.

The method according to claim 1,
Wherein the thermally conductive sleeve (200) is a ring.

The method according to claim 1,
The thermally conductive sleeve (200) is a fastener.

The method of claim 3,
Wherein said thermally conductive sleeve (200) is C-type.

The method according to claim 1,
Wherein the capillary (100) is made of plastic.

6. The method of claim 5,
Wherein the capillary tube (100) is made of polycarbonate.

The method according to claim 1,
Wherein the thermally conductive sleeve (200) is made of metal.

8. The method of claim 7,
Wherein the thermally conductive sleeve (200) is made of iron.

The method according to claim 1,
The capillary tube (100) includes an annular groove (120), and the annular groove (120) accommodates and fixes the thermally conductive sleeve (200).