KR101790250B1 - Soap composition comprising of medicinal plants - Google Patents

Abstract

The present invention relates to a soap composition containing medicinal plant extracts, and more specifically, to a soap composition which has anti-inflammatory and antibacterial activities by comprising an extract of Rumex crispus L, an extract of Sanguisorba officinalis L, and an extract of Sasa borealis (Hack.) Makino as active ingredients. The soap composition comprising the extract of Rumex crispus L, the extract of Sanguisorba officinalis L and the extract of Sasa borealis (Hack.) Makino as active ingredients according to the present invention has anti-inflammatory activities and additionally has strong antibacterial activities against Propionibacterium acnes which is a pathogen related to acnes, and Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus pyogenes which correspond to human skin pathogens. The soap composition according to the present invention can be usefully used as a new functional soap composition having an effect of improving acne skin and atopic dermatitis. Particularly, Rumex crispus L, Sanguisorba officinalis L and Sasa borealis (Hack.) Makino are edible plants, and the soap composition comprising the extracts derived from Rumex crispus L, Sanguisorba officinalis L and Sasa borealis (Hack.) Makino has an advantage that the soap composition is safe after using for a long time.

Description

[0002] Soap compositions containing medicinal plant extracts,

The present invention relates to a soap composition containing a medicinal plant extract, and more particularly to a soap composition having an anti-inflammatory activity and an antimicrobial activity, including a safflower extract, an oat extract and a sage extract as effective ingredients.

Pollution problems such as air pollution and water pollution are becoming serious due to the influence of exhaust gas generated in various industrial facilities and automobiles. As the living environment is improved, the heating efficiency of the living space is remarkably improved and the skin is kept dry The skin becomes rough or prematurely aged.

In general, the skin consists of the epidermis, the dermis, and the subcutaneous tissue from the surface. On the outermost side of the epidermis, a stratum corneum is formed by aging of epidermal cells of the skin.

The skin of such a person has the function of discharging the waste material due to the physiological action in the body to the outside of the body and the function of skin respiration for supplying oxygen necessary for the human body.

When various wastes or sebum secreted from the body are blocked in pores or sebaceous glands distributed in the skin, wastes etc. are accumulated in the epithelium of the skin, resulting in abnormal keratinization of the skin epithelium. In addition, when harmful microorganisms in the air are adsorbed on the surface of the skin, microorganisms penetrate through the pores or pores to the surface of the skin, which causes skin inflammation, and ultimately damages the skin Beauty is harmed.

Therefore, human skin should always be kept clean and maintained in order to harmonize with physiological function of human body, and when this process is established, healthy and elastic skin can be maintained.

Thus, one of the specific methods for cleansing and protecting the skin is the use of soap.

In general, soap is a detergent used to remove foreign substances such as body wash, kerosene, and so on during washing and bathing. It is used for washing soap and cloth for cleansing and bathing It can be divided into soap. The essential efficacy in soap is excellent detergency. However, as life becomes more abundant and interest in health is heightened, demands for efficacy other than detergency are increasing.

The cleansing soap consists mainly of vegetable oils and surfactants. Based on these ingredients, various functional substances are added to make various kinds of cleansing soap. That is, depending on the added functional material, ginseng soap containing ginseng, milk soap containing milk, mugwort soap containing mugwort, cucumber soap containing cucumber, and elvan soap containing fine kerosene powder can be categorized into There are so many that can not be counted.

Such a soap is easily decomposed in the process of contacting with water and adheres well to the interface so that the surface tension of the interface is lowered, thereby separating the waste matter and the like from the interface to perform the cleaning action.

However, the skin of the human skin is suppressed from evaporation to the skin surface through the pores of the sebaceous gland, and the elasticity of the skin is maintained. However, if the pore is blocked by the abnormal keratinization of the skin epithelium, When the anaerobic strain penetrates into the pores, inflammation occurs and various skin diseases such as acne are caused. In addition, even if the sebum is well discharged from the surface of the skin, if the time passes after the cleaning, microorganisms in the air propagate on the surface of the skin to expose the sebum, thereby deteriorating the surface of the skin.

In order to solve such a problem, efforts to develop a functional soap having various effects for improving health have been continuously carried out.

Accordingly, the present inventor has searched for a medicinal plant which can be applied to soap as a natural material having excellent antimicrobial activity against acne-inducing bacteria and aerobic skin-bearing bacteria, and found that the medicinal plants which can be applied to soap include a spear extract, an oat extract and a Sasa extract The soap composition has anti-inflammatory activity and is also effective against acne-causing bacteria such as Propionibacterium acnes and Staphylococcus aureus , Staphylococcus epidermidis , and purulent streptococcus, ( Sreptococcus pyogenes ), and thus the present invention has been completed.

Korean Patent Publication No. 10-1995-0003432 Korean Patent No. 10-1065608

Accordingly, it is an object of the present invention to provide a soap composition which is stable to human body and has excellent antimicrobial activity against acne-inducing bacteria and skin topical bacteria even when used for a long time by using natural medicinal plant extracts.

In order to achieve the above-mentioned object of the present invention,

The present invention provides a soap composition comprising one or more extracts selected from the group consisting of Asteraceae Extract, Oat Extract, and Sasa Extract.

In one embodiment of the present invention, the composition may include all or part of an extract of Alaska pollack, an extract of Osiris, and a sage extract.

In one embodiment of the present invention, the extracts of Rhododendron syrup extract, oat extract and Sasa extract may be contained at a weight ratio of 1: 2: 2.

In one embodiment of the present invention, the extract may be contained in an amount of 1 to 10 parts by weight based on 100 parts by weight of the total soap composition.

In one embodiment of the present invention, the composition is at least one selected from the group consisting of a soap chip, a foaming agent, a natural fatty acid, a moisturizer, sodium cellulose, sodium chloride, a pigment, a flavoring agent, a bleaching agent, an antiseptic agent, As shown in FIG.

In one embodiment of the present invention, the composition may have a liquid, cream, paste or solid form.

In one embodiment of the present invention, the composition may have anti-inflammatory activity and antibacterial activity.

In one embodiment of the present invention, the antimicrobial activity may be an antimicrobial activity against acne-causing bacteria and aerobic skin-bearing bacteria.

In one embodiment of the present invention, the acne-causing bacterium may be P. bacterium acnes.

In one embodiment of the present invention, the aerobic dermatophytes may be Staphylococcus aureus , Staphylococcus epidermidis , or Sreptococcus pyogenes .

The present invention relates to a soap composition comprising a safflower extract, an oat extract and a Sasa extract, which has an anti-inflammatory activity and is also effective against acne-causing bacteria such as Propionibacterium acnes and Staphylococcus aureus It has a strong antimicrobial activity against Staphylococcus aureus , Staphylococcus epidermidis and Sreptococcus pyogenes and can be effectively used as a novel functional soap composition having an effect for improving acne skin and atopic dermatitis . In particular, the soap compositions of the present invention comprising extracts derived therefrom, which are edible plants, have a safety advantage over long-term use.

FIG. 1 is a graph showing inhibition of nitric oxide (NO) production according to the application of the present invention to the mouse macrophages (RAW264.7 cells) according to the application of the present invention, oat extract, sasa extract and mixtures thereof (Cont: 200: the experimental group treated with 200 μg / ml of the extract of the present invention and not treated with LPS).
2A to 2C are photographs showing the medicinal plant extract (before powdering) of the present invention.
3 is a photograph showing a soap product produced using the soap composition of the present invention.

The present invention is characterized by providing a soap composition comprising at least one extract selected from the group consisting of a safflower extract, an oat extract, and a sage extract.

"Curly dock" referred to herein is the scientific name Rumex crispus L. It is called "Yangjingun", "Songgui", "opposition ear" etc. It grows frequently in the wet areas of the fields and mountain slopes of the country. It has bloomed roots in the ground and the thick stem grows up to 30-80cm There are many vertical lines, and the backlight turns on the green background. By the time the young leaves grow up, they look tall and tiny. In spring, young seeds are eaten as herbs and rooted as medicine. Examples of such molluscs include ascorbic acid, crysopanol, emodin, anthraquinone derivatives, calcium monacalcium, essential oils, oxymetalanthroquinone, chrysopanic acid, anthraglycoside and the like.

"Sanguisorba officinalis' scientific names mentioned herein are the Sanguisorba officinalis L. is a perennial plant of Rosaceae Rosaceae which grows in mountains or fields. The roots in the coarse rootstock are thickened in a cylindrical shape with rounded ends at both ends. Leaves are alternate phyllotaxis, and the root leaves are 7 ~ 11 small leaves, with petiole and small petiole. The small leaf is long oval, the edge has sawtooth, the stem leaf becomes smaller and the stem disappears. Flowers bloom from July to September and are reddish in color, and run on water inflorescence. Flower ears are elliptical or inverted ovate elliptical and begin to bloom from the top. There are four calyxes and stamens, no petals, and anthers are dark brown. The fruit is achene, ripened in October, square, and covered with calyx. There are various types of oat, oat, long oat, big oat, mountain oat, common oat, sour oat, dume oak, bead oat, and cloud oat. In addition, the roots are called zyu (root) in one room, and they are used for convergence, fever, diarrhea, dysentery, hemostasis, excessive menstruation, hemoptysis, burns and burns and contain 17% of tannins and saponins. It is distributed in Korea, China, Eastern Siberia, Japan and Kamchatka.

Sasa borealis (Hack.) Makino . Is the smallest bamboo of the bamboo. It is a small bamboo, and it grows thickly in the mountains of the southern part of Korea. Sasa bamboo is an herb with remarkable potency that far exceeds that of ginseng according to Dongbokbo, Bonchochangmok, and Goddess Maternal. It is rare that bamboo is the most resistant to the disease, and the intractable diseases such as diabetes, hypertension and gastric ulcer are completely cured do. In addition, Sasa bamboo has been reported to reduce fever, remove poison, eliminate sputum, urinate well, inhibit cancer cells, and the like.

The present inventor has searched for a medicinal plant which can be applied to soap as a natural material having excellent anti-inflammatory activity and antimicrobial activity. As a result, it has been found that a soap composition comprising a safflower extract, an oat extract and / or a Sasa extract, Propionibacterium acnes) and aerobic skin topical bacteria (Staphylococcus aureus, Staphylococcus epidermidis and purulent streptococcus).

Therefore, the composition of the present invention, which comprises at least one extract selected from the group consisting of Asteraceae extract, Osiris extract and Sasa extract, can be effectively used as a composition for skin application.

The extract according to the present invention can be obtained by extracting and isolating from nature using an extraction and separation method known in the art, and the 'extract' defined in the present invention can be obtained from a medicinal plant For example, a crude extract, a polar solvent-soluble extract or a non-polar solvent-soluble extract of the medicinal plant.

As an appropriate solvent for extracting the extract from the medicinal plants (spearmint, oat, saddle), any pharmaceutically acceptable organic solvent may be used, and water or an organic solvent may be used, including, but not limited to, For example, alcohols having 1 to 4 carbon atoms, such as purified water (water), methanol, ethanol, propanol, isopropanol, butanol, acetone, Various solvents such as ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane may be used alone or in combination . Preferably, water or ethanol is used.

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method. The method for producing the medicinal plant extract of the present invention is not limited, and any known method can be used.

For example, the medicinal plants (spearmint, oat, and sage) contained in the composition of the present invention may be prepared by subjecting the primary extract extracted by the hot water extraction or solvent extraction method described above to an additional process such as vacuum distillation, freeze drying, And can be produced in a powder state. Further, the primary extract can be further purified by using various chromatographies such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography and the like, You can get it.

Therefore, the medicinal plants (spruce, oat, and sage) in the present invention include all extracts, fractions, and purified products obtained in each step of extraction, fractionation, or purification, and diluted solutions, concentrates, or dried products thereof.

In the present invention, all of the three medicaments (Spruce, Oasi, Sasae) may be put together and extracted, or they may be separately mixed and used. However, efficient extraction of the active ingredient and extraction of the subcomponents during mixed extraction of medicinal plants It is preferable to use them separately and then mix them together.

The method for preparing the medicinal plants (spearmint, oat, and sage) extract according to an embodiment of the present invention will be described in more detail as follows.

5-10 times of weight of water was added to each of the shiitake, oacule and bupropar at a temperature of 50-60 ° C. When the amount of the aqueous solution became 1/3, the extract solution except for the shredded was firstly obtained, After the addition at the initial dose, the mixture was terminated at a temperature of 50-60 ° C until the amount of the aqueous solution became 1/3, to thereby obtain a second extract of Cryptomeria japonica extract, Oatmeal extract and Sasa extract. The first extract and the second extract thus obtained were combined, filtered, concentrated and lyophilized, and finally, each of the extracts of Saury, Oat and Sasa extract of the present invention was prepared in powder form.

The three dried medicinal plants (Phragmites, Oasi, Sasae) can be extracted all at once, or they can be mixed after they are extracted. However, efficient extraction of active ingredients and extraction of subcomponents during mixed extraction of medicinal plants are minimized To do this, individual extraction and mixing methods were followed.

The extracts of the present invention, oat extract, sasa extract and mixtures thereof have excellent anti-inflammatory activity. In addition, in the case of the soap composition prepared using the above extract, Staphylococcus aureus , Staphylococcus epidermidis , and purulent streptococcus, which are causative agents of acne (Propionibacterium acnes) ( Sreptococcus pyogenes ).

In one embodiment of the present invention, the composition may contain a mixture of Alaska pollack extract, oat extract and Sasa extract at a weight ratio of 1-2: 1-2: 1-2, And Sasa extract in a weight ratio of 1: 2: 2.

Therefore, the composition of the present invention comprising at least one extract selected from the group consisting of the extracts of Rhododendron japonica extract, oat extract and Sasa extract is useful as a functional soap composition having skin inflammation and acne prevention / improvement / therapeutic effect Can be used.

The composition of the present invention may contain 0.01 to 10.0% by weight based on the total weight of the soap composition, and may be contained in the composition at a concentration of 1 μg / ml to 10000 μg / ml.

In one embodiment of the present invention, the soap composition may contain, in addition to the active ingredient, a soap chip, a foaming agent, a natural fatty acid, a moisturizer, sodium cellulose, sodium chloride, a pigment, a flavoring, a surfactant, a bleaching agent, an antiseptic, And at least one member selected from the group consisting of

The soap composition of the present invention may contain a soap base composition together with the above-mentioned active ingredients (rosemary extract, oat extract, sage extract).

As used herein, a soap base composition is a composition of constituent materials that form the basis of a soap, and may generally comprise constituents of the soap used in the art.

The soap base composition may comprise at least one member selected from the group consisting of soap chips, foaming agents, moisturizing agents, sodium chloride, coloring matters, fragrances, bleaches, preservatives, antioxidants, discoloration inhibitors and purified water.

The term 'soap chip' as used herein refers to a soap chip or soap noodle used in a general soap manufacturing process and usually comprises a plant factory in a local (Malaysia or Philippines) port where a palm or coconut oil is immediately produced Soap clusters are called soap chips.

As used herein, the term " foaming agent " refers to a substance added to a solution or a raw material to form a stable foam, and a solution-type foaming agent is a kind of surfactant. Examples include sodium laurate, sodium cocoyl isethionate, sodium lauroyl sarcosinate, sarcosinates, potassium laurate, sodium laurate, Anionic surfactants such as lauric acid and sulfosuccinates, glutamic acid and the like; A nonionic surfactant such as Cocamido diethanolamide, alkyl polyglucoside, Polysorbate 20, PEG-100 Stearate and Polysorbate 60, ; And amphoteric surfactants such as cocoamidopropyl betaine, and the like can be suitably selected by those skilled in the art. Meanwhile, in one embodiment of the present invention, sodium cocoyl isethionate, sodium lauroyl sarcosinate and potassium laurate are mixed with the foaming agent Can be used.

The 'natural fatty acid' referred to in the present specification is a naturally occurring fatty acid, and when used in combination with a foaming agent, serves as a foam assistant to enable fine and small bubbles to be abundantly generated. Examples thereof include Myristic acid, Palmitic acid, Stearic acid, Lauric acid and the like, and those skilled in the art can appropriately select them. Meanwhile, in one embodiment of the present invention, lauric acid may be used as the natural fatty acid, and when mixed with lauric acid as a foaming agent, minute small bubbles are abundantly generated, and skin, lipid, keratin, The ability to adsorb with various waste materials becomes large and can be removed very effectively.

The 'moisturizing agent' referred to in the present specification includes galactoarabinan, argan oil, shea butter, hyaluronic acid, glycerin and the like as a component for enhancing moisture retention of the skin, and a person skilled in the art can appropriately select it. Meanwhile, in one embodiment of the present invention, shea butter, argan oil, sodium hyaluronic acid and glycerin may be mixed with the moisturizing agent.

The 'sodium cellulose' as a constituent component of the soap base composition of the present invention, when added together with the foaming agent, can lead to a much longer holding time of the small bubbles, thereby increasing the effective function of the mixing foam agent in the skin do.

As a component of the soap base composition of the present invention, 'sodium chloride' serves as a solid agent for making the soap hard.

The soap composition of the present invention comprising the above-mentioned active ingredient and soap base may be in the form of a liquid, cream, paste, or solid form, but it is a concept including any type of formulation as long as it can function as a soap composition It does not particularly limit the kind thereof.

In one embodiment of the present invention, the medicinal plant extract may contain 1-10 parts by weight of the horsetail extract, oat extract, bosom extract and mixtures thereof, based on 100 parts by weight of the total composition of the soap composition; The soap chips may be included in 60 to 70 parts by weight based on 100 parts by weight of the total of the soap composition; The foaming agent may be included in an amount of 1 to 30 parts by weight based on 100 parts by weight of the total amount of the soap composition; The natural fatty acid may be contained in an amount of 1-20 parts by weight based on 100 parts by weight of the total of the soap composition; The moisturizing agent may be included in an amount of 1 to 5 parts by weight based on 100 parts by weight of the total amount of the soap composition; The sodium cellulose may be included in an amount of 0.1 to 10 parts by weight based on 100 parts by weight of the total amount of the soap composition; Sodium chloride may be included in an amount of 0.1 to 5 parts by weight based on 100 parts by weight of the total amount of the soap composition.

The soap composition of the present invention as described above can be produced as a soap product that can be commercially available through conventional manufacturing methods.

Specifically, a) a herbal base composition comprising at least one selected from the group consisting of a soap chip, a foam, a moisturizer, sodium chloride, a pigment, a flavor, a bleach, an antiseptic, an antioxidant, a discoloration inhibitor and purified water, (Syrup extract, oat extract, bovine extract or a mixture thereof) to prepare a soap composition; and b) subjecting the soap composition to a step of subjecting it to a process such as a plodding, a soap bar, a cutting, and a stamping process.

In the step a) of the present invention, sodium lauryl sarcosinate and potassium laurate are added after soap chips, sodium cocoyl ischinate, lauric acid and an appropriate amount of a dye diluent are added and mixed thoroughly so that the dye is evenly deposited After mixing; Shea butter, argan oil, sodium hyaluronic acid and glycerin, which are moisturizing agents, are added sequentially and uniformly mixed; Sequentially, sodium cellulose, medicinal plant extract, sodium chloride, perfume, and the mixture is thoroughly stirred to prepare a soap composition.

In step b) of the present invention, the soap composition prepared in step a) is put into a soap maker, and when the screw pushes the soap composition out of the perforated plate, the cutter finely cut the noodle is repeated three or more times Next, at the end, a strong vacuum is applied to completely remove the air inside the soap, thereby increasing the density and reducing the air gap. At the end of the last step, the bar is cut into long bars at intervals of about 50 cm. In the molding step, the bar-shaped soap is pressed by a molding machine consisting of four soap molds, and the soap formed by vacuum adsorption is taken out.

The molded soap manufactured through the above steps may be completed with an inlay wrapping step of wrapping the wrapped soap with a thin paper and a soap product through a carton wrapping step in which the inlay wrapping is a small box.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

< Example  1>

Preparation of medicinal plant extract

<1-1> Preparation of Syrup extract

The spider used in the present invention was directly washed in October 2015 at Sunggasan in Cheonan and cleaned with water. The leaves and roots of the spikelets were both used. The spikelet leaves were cleaned with water and then prepared 3-4 mm. The roots were cleaned with water and then cut to 1-2 mm and then dried. The drying process can be carried out for 3 to 7 days on shrubs, or dried for 10-14 hours using a food drier until the moisture content is within 10%.

5 kg of water was added to 1 kg of syrup prepared by the above procedure, and water was soaked in the syringe for 1 hour. After the water was completely immersed in the syringe, it was withdrawn at 50-60 ° C for 7-8 hours, / 3, the extract was firstly obtained except for the gherkin, and 5 kg of water was added again to the gherkin and the mixture was cut at 50-60 ° C for 7 to 8 hours. When the volume of the solution reached 1/3 of the initial amount of the solution, Secondary extracts were obtained. The primary extract and the secondary extract thus obtained were combined, filtered, concentrated and lyophilized, and finally, the powdery extract of the present invention was prepared in powder form.

&Lt; 1-2 >

The ointment used in the present invention was directly removed from Gwangdeok Mountain in Cheonan City in November 2015 and cleaned with water. The washed oat root was dried, and then the oat extract was prepared in the same manner as in Example <1-1>.

<1-3> Preparation of Sasa extract

The sludge used in the present invention was directly removed from Kwangdeok Mountain in Cheonan City in November 2015 and cleaned with water. After the washed sage leaves were dried, Sasa extract was prepared in the same manner as in Example <1-1>.

Each of the extracts thus obtained was used as a sample for the following experiment while being stored at 4 ° C in a refrigerator.

< Example  2>

Anti-inflammatory effect of medicinal plant extracts

<2-1> Nitric oxide formation inhibitory activity

In order to confirm the anti-inflammatory effect of the medicinal plant extract of the present invention prepared in Example 1, the inhibitory activity of NO production was measured.

Nitric oxide (NO) is an important intracellular proinflammatory mediator, which reacts with a superoxide radical to produce peroxynitrite anion (ONOO-). The nitrite peroxide thus produced induces inflammatory activity and causes arthritis, ulcers It is known to cause a variety of pathological conditions such as chronic colitis and systemic lupus erythematosus. Therefore, the inflammatory reaction can be inhibited by a mechanism of inhibiting nitric oxide (NO) production.

In this experiment, the extract of the present invention was pretreated with mouse macrophages (RAW264.7 cells), and the level of nitrite (indicator of nitric oxide) was measured by treating LPS with nitric acid, Were measured using a colorimetric assay. L-arginine, a substrate for nitric oxide (NO), turns into L-citrulline and nitrogen monoxide, which quickly turns into stable nitrogen dioxide, nitrite, and nitrate. The Greek reagent chemically reacts with nitrite to form a purple azo salt, which is consistent with the concentration of nitrogen monoxide, and can be determined by measuring the nitrite concentration from the concentration of the azo salt and measuring its maximum absorption at 540 nm.

In detail, mouse macrophages (RAW 264.7 cells) were divided into 96-well plates at a density of 5.0 × 10 ⁵ cells / well and cultured. Then, as a medicinal plant extract of the present invention, Sophorae extract, Osiris extract, (1: 1: 1) was treated at a concentration of 100 μg / ml, treated with 1 μg / ml of LPS and incubated in a 5% CO ₂ incubator at 37 ° C. for 24 hours. Was measured with a grease reagent. In other words, absorbance of nitrite (NO2-) was measured at 540 nm with an ELISA microplate reader (Tecan, USA) after 100 μl of cell culture solution and 100 μl of 6 mg / ml grease reagent were mixed and reacted. Absorbance was measured with sodium nitrite (NaNO ₂ ) to obtain a standard curve, and the concentration of NO was calculated.

As a result, as shown in Fig. 1, it was found that the nitrite level was reduced in the experimental group treated with the medicinal plant extract of the present invention compared with the experimental group (the nitrite content was increased 6.5 times) treated with LPS only in the macrophages, It was confirmed that the nitrite level was reduced to 25.7% in the experimental group treated with the mixture of the three medicinal plant extracts of the present invention. For reference, in FIG. 1, Cont represents the untreated group treated with LPS and the medicinal plant extract, and 100 indicates the experimental group treated with 100 μg / ml of the medicinal plant extract of the present invention and not treated with LPS.

From the above results, it was confirmed that the medicinal plant extract of the present invention can effectively inhibit the production of nitrogen oxides. In particular, it was confirmed that the mixture of three kinds of extracts is superior to the extract alone.

<2-2> Carrageenan  Pediatric edema Carrageenan  Foot Edema)

As a medicinal plant extract of the present invention, 100 mg / kg of Sophora rhamnospora extract, oat extract, and Sasa extract alone and a mixture thereof were administered to the abdominal cavity of rats. After 1 hour, 0.5 ml of a 0.1% carrageenan solution was injected into the back soles of the experimental animals To induce inflammation. Changes in the amount of paw blood immediately after injection of carrageenan and 4 hours after injection were measured, and the results are shown in Table 1 below.

% Inhibition rate = (1 -? V treated group /? V control group) x 100

(? V: change in foot volume)

The edema inhibition rate according to the medicinal plant extract treatment of the present invention extract Mixing ratio % Inhibition rate Horseradish extract - 48.2 Oat extract - 60.2 Sasa extract - 40.0 Shout + Oylum 1: 1 54.8 Shout 1: 1 40.8 Oyafuri + Sage 1: 1 48.7 Shouting 1: 1: 1 82.4 Shouting 1: 2: 1 80.6 Shouting 2: 1: 1 82.2 Shouting 1: 2: 2 88.5 Shouting 2: 2: 1 84.6 Shouting 2: 1: 2 78.7

From the above results, it was confirmed that the medicinal plant extract of the present invention can suppress edema induced by inflammation. In particular, it was confirmed that the mixture of three kinds of extracts is superior in the anti-inflammatory effect compared to the single extract.

< Example  3>

Soap composition manufacturing

A soap composition having the composition ratios shown in Table 2 was prepared.

Soap composition Raw material name Content (% by weight) Where to buy Soap chip 60-70 Musim mas Sodium cocoyl isethionate 130 Akzonobel / Elfan AT 84G Sodium lauroyl sarcosinate 110 Miamil L95 Potassium laurate 120 Zenta Laurick Eshid 1-20 LG Household & Health Care Shea Butter 0.15 Kalichem Argan oil 0.15 Kalichem Sodium hyaluronic acid 0.15 Kalichem glycerin 0.15 LG Household & Health Care Sodium cellulose 0.110 Bor Medicinal plant extract 115 - Sodium chloride 0.15 One week salt Pigment 0.0001-0.001 Bor Spices 0.55 Hanbong pyong Purified water to 100 -

First, soap chips, sodium cocoyl succinate, lauric acid and an appropriate amount of a coloring matter diluent were added, and the mixture was thoroughly mixed to uniformly deposit the coloring matter. Then sodium lauroyl sarcosinate and potassium laurate were added and mixed. After that, shea butter, argan oil, sodium hyaluronic acid and glycerin, which are moisturizing agents, were added sequentially and mixed uniformly. After thorough mixing, sodium cellulose, medicinal plant extract, sodium chloride and flavoring were sequentially added in order, and the mixture was thoroughly stirred to prepare a soap composition. For reference, the coloring matter was prepared by diluting Orange No. 2 (CI 15510) purchased from Borak with 0.1% concentration and using 3% (content: 0.003%), Respectively.

Then, the final solid soap was produced by subjecting the soap making machine to the processes of plodding, soap bar, cutting, and stamping.

< Example  4>

Evaluation of workability of soap composition

The workability was evaluated according to the addition ratio of the medicinal plant extract contained in the soap composition of the present invention. For this purpose, the soap was adhered to the machine during the soap manufacturing process and the sticky dentifrice was classified into five stages: very good, excellent, normal, poor, and very poor.

Workability according to addition ratio of medicinal plant extract contained in soap composition Manufacturing example Horseradish extract
(Unit: wt%) Oat extract
(Unit: wt%) Sasa extract
(Unit: wt%) Workability One 0.1 - - Good 2 0.5 - - Good 3 1.0 - - Good 4 10.0 - - Good 5 15.0 - - No action 6 - 0.1 - Good 7 - 0.5 - Good 8 - 1.0 - Good 9 - 10.0 - Good 10 - 15.0 - No action 11 - - 0.1 Good 12 - - 0.5 Good 13 - - 1.0 Good 14 - - 10.0 Good 15 - - 15.0 No action Refers to the weight percentage of each of the above-mentioned spearmint, oacule, and sage extract added to the total soap composition

As shown in Table 3, when the addition ratio of the medicinal plant extract contained in the soap composition is less than 15%, the workability is good, but when it is more than 15%, the workability of the soap composition is inferior.

< Example  5>

Antimicrobial activity of acne induced bacteria by addition of medicinal plant extracts

In this experiment, the anti-microbial properties of the soap composition prepared by adding the medicinal plant extract of the present invention prepared in Example 1 to actual acne-causing bacteria were investigated.

The antimicrobial effect of P. acnes , which is known as a cause of acne, was determined. The minimum inhibitory concentration (MIC) for P. acnes was determined by liquid culture dilution method.

Using the composition ratios in Table 4 below, the inventive soap compositions prepared in analogy to the method of Example 3 were diluted 100-fold with sterile distilled water, diluted 2-fold to 28 in 5 ml of liquid medium and used as samples . Propionibacterium acnes ( P. acnes ) was cultured in TS (trypticase strain) broth supplemented with 5% sheep blood at 35 ° C for 48 hours in anaerobic condition and then used for the test. Each inoculum was adjusted to 2 × 10 ⁶ CFU / well and incubated with the sample for 24 hours. The growth of the bacteria was observed with the naked eye and a microscope. The absorbance at 540 nm was measured and the absorbance Was determined as the minimum inhibitory concentration. The results are shown in Table 5 below.

Soap composition by type of medicinal plant extract Furtherance Example (Unit: wt%) Comparative Example Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Example 9 Soap chip 67.7 66.7 66.7 66.7 64.7 64.7 64.7 62.7 62.7 62.7 Sodium cocoyl isethionate 10 10 10 10 10 10 10 10 10 10 Sodium lauroyl sarcosinate 3 3 3 3 3 3 3 3 3 3 Potassium laurate 7 7 7 7 7 7 7 7 7 7 Laurick Eshid 5 5 5 5 5 5 5 5 5 5 Shea Butter 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Argan oil 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Sodium hyaluronic acid One One One One One One One One One One glycerin 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Sodium cellulose 3 3 3 3 3 3 3 3 3 3 Horseradish extract - One - - 3 - - 5 - - Oat extract - - One - - 3 - - 5 - Sasa extract - - One - - 3 - - 5 Sodium chloride 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Pigment 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Spices One One One One One One One One One One Purified water - - - - - - - - - -

The antibacterial activity against the acne-causing bacteria of the soap composition according to the type of medicinal plant extract (unit: MIC (μg / ml)) bacteria Comparative Example Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Example 9 P. acnes 10.2 4.8 4.0 6.2 3.2 3.0 4.2 2.2 3.0 2.8

As a result, as shown in Table 5, in the case of the soap composition containing the medicinal plant extract (Examples 1-9) in comparison with the soap composition not containing the medicinal plant extract (comparative example), in the case of the propionibacterium arc It was confirmed that the antimicrobial activity against P. acnes was excellent.

< Example  6>

Aerobic by addition of medicinal plant extract alone Skin  Antimicrobial activity measurement

In this experiment, the soap compositions prepared by adding the medicinal plant extract of the present invention prepared in Example 1 were examined for the antimicrobial activity against the aerobic skin microbes.

Staphylococcus aureus , Staphylococcus epidermidis , and Sreptococcus spp. pyogenes ) were cultured for 18 hr at 37 ° C using Muller-Hiton broth and cultured in CO ₂ incubator for S. pyogenes . Each inoculum was adjusted to be 2 x 10 ⁶ CFU / well to prepare a diluted soap composition sample (the composition of the present invention prepared similarly to the method of Example 2 above using the composition ratio shown in Table 3) 100-fold dilution, diluted 2-fold to 28 in 5 ml of a liquid medium, and used as a sample) for 18 hours. The growth of the microorganism was observed with the naked eye and a microscope, and the absorbance was measured at an absorption wavelength of 540 nm The minimum inhibitory concentration was determined to be the same as the absorbance value of the pure culture medium. The results are shown in Table 5 below.

The antimicrobial activity (unit: MIC (μg / ml)) of the soap composition of each type of medicinal plant extract against aerobic skin microbes bacteria Comparative Example Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Example 9 S. aureus - - - - 8.5 10.0 - 7.0 8.0 8.0 S. epidermidis - - - - 6.2 6.0 8.0 6.0 5.8 6.2 S. pyogenes - - - - - - - - - -

As a result, as shown in Table 6, antimicrobial activity against Staphylococcus aureus and Staphylococcus epidermidis was observed only in a soap composition containing 3% by weight or 5% by weight of a medicinal plant extract , But did not show antimicrobial activity in other Examples. In addition, it was confirmed that the antimicrobial activity against purulent streptococci ( Sreptococcus pyogenes ) was not observed in all experimental groups.

< Example  7>

Measurement of Antimicrobial Activity of Acne Inducing Bacteria by Addition of Medicinal Plant Extract

In this experiment, the antibacterial properties of the soap composition prepared by mixing the medicinal plant extract of the present invention prepared in Example 1 with respect to acne-causing bacteria were examined.

Antimicrobial activity against acne-causing bacteria was evaluated in the same manner as in Example 4 using the composition of the present invention prepared using the composition ratios shown in Table 7 below.

A soap composition mixed with a medicinal plant extract Furtherance Example (Unit: wt%) Comparative Example Example 10 Example 11 Example 12 Example 13 Example 14 Example 15 Example 16 Example 17 Example 18 Soap chip 67.7 65.7 65.7 65.7 64.7 63.7 63.7 62.7 62.7 62.7 Sodium cocoyl isethionate 10 10 10 10 10 10 10 10 10 10 Sodium lauroyl sarcosinate 3 3 3 3 3 3 3 3 3 3 Potassium laurate 7 7 7 7 7 7 7 7 7 7 Laurick Eshid 5 5 5 5 5 5 5 5 5 5 Shea Butter 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Argan oil 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Sodium hyaluronic acid One One One One One One One One One One glycerin 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Sodium cellulose 3 3 3 3 3 3 3 3 3 3 Horseradish extract - One One - One One 2 One 2 2 Oat extract - One - One One 2 One 2 2 One Sasa extract - - One One One One One 2 One 2 Sodium chloride 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Pigment 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Spices One One One One One One One One One One Purified water - - - - - - - - - -

The antimicrobial activity against the acne-causing bacteria of the soap composition according to the addition of the medicinal plant extract (unit: MIC (μg / ml)) bacteria Comparative Example Example 10 Example 11 Example 12 Example 13 Example 14 Example 15 Example 16 Example 17 Example 18 P. acnes 10.2 3.4 3.8 3.8 3.0 2.0 2.4 0.8 2.0 2.2

As a result, as shown in Table 8, it was confirmed that the antibacterial activity against Propionibacterium acnes ( P. acnes ), an acne-causing microorganism, was excellent in Examples 13-18 in which three kinds of medicinal plant extracts of the present invention were mixed And showed excellent antimicrobial effect against acne-inducing bacteria in a soap composition containing a mixture of 1: 2: 2 by weight of the extracts of Cryptomeria japonica, Osiris extract and Sasa extract.

< Example  8>

Aerobic activity of mixed medicinal plant extracts Skin  Antimicrobial activity measurement

In this experiment, the inventors of the present invention investigated whether the antimicrobial activity of the soap composition prepared by mixing the medicinal plant extract of the present invention prepared in Example 1 with the aerobic skin microbes was increased.

The antimicrobial activity of the aerobic skin microflora was evaluated in the same manner as in Example 5 using the soap composition of the present invention prepared using the composition ratios shown in Table 7 above.

The antimicrobial activity against aerobic skin of the soap composition according to the addition of medicinal plant extract (unit: MIC (μg / ml)) bacteria Comparative Example Example 10 Example 11 Example 12 Example 13 Example 14 Example 15 Example 16 Example 17 Example 18 S. aureus - - - - 8.2 5.8 6.2 2.2 4.0 3.2 S. epidermidis - - - - 6.8 6.0 5.2 2.0 3.8 4.4 S. pyogenes - - - - - - - 3.4 10.0 8.0

As a result, as shown in Table 9, antibacterial activity against Staphylococcus aureus and Staphylococcus epidermidis was shown in Examples 13-18 in which three kinds of medicinal plant extracts of the present invention were mixed, Purulent Streptococcus ( Sreptococcus The antimicrobial activity against pyogenes was shown in Examples 16-18, and particularly excellent antimicrobial activity in Example 16 (2-3 times higher for Sreptococcus pyogenes compared to Examples 17 and 18) Showing antimicrobial activity).

< Example  9>

The skin irritation degree of the soap composition of the present invention

1 ml of a 5% aqueous solution of a soap composition (Examples 10-18) containing the medicinal plant extract of the present invention was absorbed into the gauze portion of the bandage using a stimulant test band-aid, and then stuck on the upper part of the human arm. After 48 hours, the bandage was removed and the skin was observed 1 hour later. The evaluation method was evaluated by the following evaluation table.

The results are shown in Table 11 below. The results are shown in Table 11 below.

Skin irritation degree evaluation table Rating condition 0 There is no response to skin. One Partial skin erythema 2 Overall, skin erythema occurs. 3 Skin rashes and skin swells. 4 The skin is reddened and the skin is severely divided.

The skin irritation degree of the soap composition of the present invention Evaluation items Comparative Example Example 10 Example 11 Example 12 Example 13 Example 14 Example 15 Example 16 Example 17 Example 18 Skin irritation 0.1 0.1 0.1 0.1 0.1 0.2 0.1 0.2 0.2 0.1

As shown in Table 11, the soap composition of the present invention showed almost no skin irritation, indicating that it was stable to the skin during use.

< Example  10>

The soap composition of the present invention Moisture power , Wound healing effect, mitigating effect of itching, skin strengthening, and effect of alleviation of atopic skin hyperplasia

In the case of the soap composition (Examples 10-18) containing the medicinal plant extract of the present invention, at least five patients with atopic dermatitis of at least about 50 in respect of moisturizing power, wound healing effect, mitigating effect of itching, skin strengthening, And divided into 10 groups. The effect was evaluated in the morning and in the evening by using it in areas showing atopic dermatitis symptoms twice.

At this time, the evaluation was performed according to the criterion of excellent 10 points, excellent 8 points, average 6 points, poor 4 points, and very poor 2 points, and the results are shown in Table 12.

Evaluation of the moisturizing power, wound healing effect, itching relieving effect, skin strengthening and atopic skin alleviation effect of the soap composition of the present invention Evaluation items Comparative Example Example 10 Example 11 Example 12 Example 13 Example 14 Example 15 Example 16 Example 17 Example 18 Moisture power 8.6 9.0 9.0 9.0 8.6 8.8 9.0 9.0 8.4 8.8 Wound healing 7.0 8.0 8.2 8.0 8.4 8.8 9.0 9.2 9.0 9.0 Itching relief 5.0 7.6 7.6 7.0 8.0 8.4 8.8 9.2 8.8 9.0 Skin tightening 7.0 7.6 7.4 7.6 7.6 8.8 8.4 8.8 9.0 8.4 Atopic skin symptom relief 5.0 7.2 7.0 7.4 8.4 8.0 8.8 9.4 9.0 9.2

As shown in Table 12, it was confirmed that the soap composition of the present invention is improved in moisturizing power, wound healing effect, itching relief effect, skin strengthening effect and atopic skin symptom relief effect in comparison with the comparative example, It was confirmed that the soap composition prepared by mixing three kinds of extracts, oat extract and Sasa extract showed remarkably more itching relieving effect and atopic skin symptom relief effect.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (10)

Wherein the weight ratio of 1: 2: 2 is 1: 2: 2. delete delete The method according to claim 1,
Wherein the composition comprises 1 to 10 parts by weight based on 100 parts by weight of the total composition of the soap composition. The method according to claim 1,
The composition further comprises at least one member selected from the group consisting of a soap chip, a foaming agent, a natural fatty acid, a moisturizing agent, sodium cellulose, sodium chloride, a pigment, a flavoring agent, a surfactant, a bleaching agent, an antiseptic agent, &Lt; / RTI &gt; The method according to claim 1,
Wherein the composition has a formulation in a liquid, cream, paste or solid form. The method according to any one of claims 1, 4, 5, and 6,
The composition has anti-inflammatory activity and antimicrobial activity,
The antimicrobial activity is antimicrobial activity against P. acnes , which is an acne-causing microorganism, as well as aerobic strains of Staphylococcus aureus , Staphylococcus epidermidis , Lt; RTI ID = 0.0 &gt; ( Sreptococcus pyogenes ). &Lt; / RTI &gt; delete delete delete

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