KR101721747B1 - Purification and Conservation extension method of Zanthoxylum schinifolium oil - Google Patents

Abstract

The present invention relates to a method for efficiently purifying impurities of sancho oil and preventing rancidity, thereby increasing storage stability. Acidic colostrum removes impurities through milking after milking, but rancidity takes place in this process, and sediment and the like are visually observed, and efficient purification technology is required. In addition, acidic colostrum may cause safety problems due to rapid rancidity. The present invention provides a technique for efficiently purifying acidic colostrum in a short time and preventing rancidity by using a safe agent for the human body.

Description

Purification and storage extension method of Zanthoxylum schinifolium oil < RTI ID = 0.0 >

The present invention relates to an acidic colostrum refining agent and a preservative elongation method, and more particularly, to an acidic colostrum refining agent and a preservation extender method capable of overcoming the problem of rancidification using a safe material for human body, .

Zanthoxylum schinifolium is a deciduous shrub belonging to the genus Rutaceae and is widely known as a plant resource used for folk medicine since ancient times. In recent years, it has been widely used for bronchial, asthmatic, gastritis, and gastrointestinal tracts, and the scientific effects such as improvement of atopy and anti-inflammation are widely known.

In Patent Registration No. 10-1056475, in the present invention, the colostrum of the present invention is obtained by drying the seeds of Sancho wood, roasting it at 230 to 250 ° C for 5 to 10 minutes and then milking, drying the seeds of the Sancho wood and drying it for 10 to 20 minutes The cream for atopic skin improvement of the present invention is characterized by comprising 30.0 to 33.0% by weight of a skin conditioning agent containing an acidic colostrum; 43.0 to 44.0% by weight of solvent; 9.0 to 9.2% by weight of a surfactant; 7.8 to 8.0 wt% softener; 5.6 to 5.7% by weight of flocculant; 0.1 to 4.6% by weight of a pH adjusting agent and a natural preservative; By weight of an acidic colostrum. The cosmetic composition for improving atopic skin contains an acidic colostrum.

Also, in Laid-open Patent Publication No. 10-2006-0083915, there is provided a method for producing an oral care composition comprising the steps of:

(A) washing the sancho, the fruit of Zanthoxylum schinifolium;

(B) mixing and extracting from 5 to 25 parts by weight of an acidic acid of the step (A) per 100 parts by weight of a solvent selected from the group consisting of ethanol, methanol and a mixture thereof with water;

(C) filtering the extract of step (B) to recover the filtrate;

(D) removing the solvent from the filtrate in the step (C) to recover an acnes extract; And

(E) 0.01 to 5% by weight of a sancho extract of the step (D) in an ordinary oral hygiene product is added to an oral hygiene product and a preparation method thereof.

 However, the above conventional vegetable oils have been limited in their availability due to changes occurring during processing, cooking and storage. Generally, the vegetable oil is oxidized according to the milking method and the storage period, and such change may cause harmful substances, which may cause great problems in food hygiene. Rancidity is a phenomenon in which the quality of fatty foods is degraded during processing and storage due to various chemical and microbiological factors such as changes in smell and taste and loss of nutritional value. Therefore, it has been used in the civilization for a long time, but the refining method and the storage method are not scientifically known, which can cause problems in distribution and use.

The acidic colostrum has a high content of unsaturated fatty acids, so that the acidity of the oil is likely to occur, and the milked colostrum is not transparent in color and contains a large amount of impurities. These impurities may act as a rancid factor during the storage process of the acid plant. In general, the purification of the oil is subjected to a degumming process and a neutralization process. The deoxidation process removes the lecithin dissolved in the fat. In the deoxidation process, the free fatty acid in the crude oil is neutralized with an aqueous alkaline solution (NaOH), and the coexisting phospholipids (lecithin), viscous substance and pigment are also removed . This process is commonly performed, but different conditions are required depending on the type of maintenance. Acidic colostrum has long been used in the private sector, but no purification method has been developed.

In addition, acidic colostrum has long been used in the private sector, but it is susceptible to rancidity, but no method has been developed to prevent it. Antioxidants and the like have been proposed to increase the storage stability of oils and fats, but synthetic antioxidants have been problematic in terms of human safety and the like.

DISCLOSURE OF THE INVENTION Accordingly, the present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide a method of deoxidizing acidic colostrum to efficiently purify phospholipids and free fatty acids, and further, to reduce rancidity by adding sodium bicarbonate It has its purpose.

The present invention relates to an acidic colostrum refining agent and a preservation extendable method, wherein the acidic colostrum is defatted by mixing phosphoric acid to purify and extend shelf life.

According to the above, the defection and deoxidation can increase the commerciality of the sancho oil, and the sodium bicarbonate can prevent the acidosis of the sancho oil. Especially, the addition of deoxidation and deoxidation and the addition of sodium bicarbonate can greatly contribute to the storage and distribution of the acidolide. Furthermore, the preserved acidic colostrum can be used as an ingredient of health functional foods, functional beverages, and pharmaceuticals.

Fig. 1 shows changes in acid value and turbidity depending on the number of days of squeezed sancho oil.
FIG. 2 shows the content of phosphoric acid in the acidic acid according to the phosphoric acid treatment,
FIG. 3 shows the change of the acid value of the sancho oil by sodium hydroxide treatment,
Fig. 4 shows changes in the content of unsaturated fatty acids in the acidolide according to sodium hydroxide treatment,
FIG. 5 shows changes in unsaturated fatty acids of the oil of Sancho oil according to the purification process,
FIG. 6 shows changes in color difference, polyphenol content, and antioxidative activity caused by defection and deoxidation,
FIG. 7 shows changes in the acid value change of the acidic acid with use of sodium bicarbonate and storage days.

The present invention relates to an acidic colostrum refining agent and a shelf life extension method for refining acidic colostrum by defatting acidic colostrum to improve its shelf life, wherein the acidic colostrum is further mixed with phosphoric acid and acetic acid, wherein 99.4 to 99.9% 0.05 to 0.3% by weight of phosphoric acid and 0.05 to 0.3% by weight of acetic acid, mixing the above-mentioned phosphoric acid and further adding sodium hydroxide (NaOH) to the defatted and purified acidic colostrum to further purify impurities, , Sodium hydroxide (NaOH) is mixed at a concentration of 1 to 3N, sodium phosphate bicarbonate is added to the defatted and purified acidic colostrum by mixing the phosphoric acid, and the sodium bicarbonate is added in an amount of 0.5 to 2 By weight, and the mixture is kept in a dark state at a temperature of 25 占 占 폚 to defend against acid rancidity.

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The present invention will be described in detail with reference to the accompanying drawings. Fig. 1 shows changes in acid value and turbidity depending on the number of days of squeezed sancho oil. FIG. 2 shows the phosphoric acid content of the acidophilic acid according to the phosphoric acid treatment, FIG. 3 shows the acid value change of the acidic acid with the sodium hydroxide treatment, FIG. 4 shows the content of the unsaturated fatty acid in the acidic acid according to the sodium hydroxide treatment, FIG. 5 shows changes in unsaturated fatty acids of the acidosis oil according to the purification process. FIG. 6 shows the change in color difference, polyphenol content and antioxidant activity due to the elimination and deoxidation. FIG. And the change in the acid value change.

According to one aspect of the present invention, a method for efficiently purifying various impurities contained in an acidic colostrum is provided.

Further, according to two aspects of the present invention,

The treatment of sodium bicarbonate, which is widely used in foods and the like, provides a method of preventing rancidity.

Hereinafter, the present invention will be described in detail.

First, after milking the colostrum, it was allowed to stand at room temperature to remove impurities, and the changes in the acidity and turbidity of the acidophilus caused by the process were measured. The conditions for effectively purifying the acidic colostrum by deacidification were investigated.

       0.05-0.3% by weight of phosphoric acid was mixed with the acidic colostrum in a proper ratio and the purification efficiency was investigated.

      In addition, an appropriate concentration for effectively removing impurities was examined using sodium hydroxide of 1-3 N concentration. The effect of purification and unsaturated fatty acid, polyphenol, antioxidant activity and color difference were investigated through the deionization and deoxidation process.

Finally, sodium bicarbonate is added at 0.5, 1.0, and 2 wt% concentration as a suitable material to retard or prevent rancidity, and the rancidity is investigated.

As a result, it was confirmed that deoxidation process using phosphoric acid and deoxidation process using sodium hydroxide can purify acid colostrum more efficiently. In addition, it proved that sodium bicarbonate is very effective for prevention of sickness rancidity, which causes problems such as storage and so on.

Hereinafter, the purification method and the material of the present invention will be described in detail by way of examples, with reference to the method of strongly suppressing impurity purification and acid corrosion of the acid pickling oil. It is to be understood that the present invention is not limited thereto.

[Example 1] Test of acid value and turbidity according to the politics of Sancho

The acidic colostrum used in the present invention was obtained by milking onion seeds produced in Hadong A farm in Gyeongnam Province.

In the traditional method, the colostrum is left at room temperature after oil extraction to precipitate the fluoride.

In the present invention, in order to investigate changes in acid value and turbidity in the process, compressed colostrum oil was allowed to stand at 25 DEG C for 7 days and acid value and turbidity were measured.

In the present invention, the measurement of the acid value of the sancho oil was carried out by the method of AOAC (1984). 20 ml of a mixture of ether: ethanol (2: 1, v / v) and 17 μl of 1% phenolphthalein were added to a 100 ml Erlenmeyer flask and titrated with 0.1 N KOH to determine the amount of KOH in the experiment. Then, a 100 ml Erlenmeyer flask was placed on the electronic balance, zero point was added, and 1 g of an acidic colostrum sample to be measured was added. 20 ml of an ether: ethanol (2: 1, v / v) mixture was added to completely dissolve the sample Next, titration was performed using 0.1 N KOH to determine the appropriate amount of the experimental group. The backwash of KOH was calculated by using 0.2-0.3 g of benzoic acid (Equation 1). When the titration was proper, the amount of KOH was maintained at the end point for 1 minute, and the acid value of the acid was measured using the KOH titration amount (Equation 2).

As a result, the acidity of acidified colostrum showed a tendency to increase with increasing number of days of incubation at room temperature in order to sediment the precipitate, and the turbidity decreased inversely (Fig. 1).

Even if it is allowed to stand at a low temperature in order to prevent the acid corrosion of the acidic acid, it is difficult to remove the impurities because the viscosity of the acidic acid oil is increased. Therefore, it is necessary to develop a method to efficiently purify acidic colostrum.

[Example 2] Purification test of Sansho oil according to defection and deoxidation process

A. Test for pickling of the pickles

100 g of colostrum was quantified, and then 10 ml of 0.05% by weight of 0.05-0.3% by weight of phosphoric acid and acetic acid was added at intervals of 10% by weight, and the efficiency was measured according to the following method. The acetic acid is used in parallel with phosphoric acid and has a large effect of removing impurities in the fat, especially protein and phospholipid.

5N sulfuric acid, 0.01M potassium antimonyl tatarate, 4% ammonium molybdate and 1M ascorbic acid were prepared, respectively. 50 ml of 5N sulfuric acid, 5 ml of potassium antimonyl tartrate. 15 ml of ammonium molybdate. 30 ml of ascorbic acid was added to make 100 ml of total ascorbic acid, then 5 ml of colostrum was mixed with 0.8 ml of mixed reagent. Then, the mixture was allowed to stand for 30 minutes, and 2 ml of 95% ethanol was added thereto for vertexing, and the absorbance was measured by an 880 nm ultraviolet spectrometer. The standard calibration curve of phosphoric acid was prepared by adding 0.8 ml of the mixed reagent with KH ₂ PO ₄ and incubating for 30 minutes. Then, 2 ml of 95% ethanol was mixed and the total phosphoric acid content was determined by measuring the absorbance at 880 nm. The standard curve for effective phosphoric acid was y = 0.007x + 0.0119 and R ² = 0.9989.

As a result, when the phosphoric acid was used in the deoxidation process of the acid pickling, the efficiency of the deodorization was increased in a concentration dependent manner up to the concentration of 0.15 wt%, but the efficiency decreased in the treatment of 0.2 wt% (FIG. In addition, when phosphoric acid concentration of 0.2 wt% or more was used, the efficiency of delicacy of the soybean paste was lower than that of 0.2 wt% treatment. The highest concentration of phosphoric acid was found to be 0.15 wt%, and the efficiency was about 72.5%.

B. Deoxidation test of Sancho oil

100 g of colostrum was quantitatively determined, and sodium hydroxide (NaOH) was added at a concentration of 1-3 N to determine the concentration of sodium hydroxide effective for yield and acid value. At this time, the concentration of sodium hydroxide to be treated was 0.2-1%. The acidic colostrum containing sodium hydroxide was reacted in a water bath of 80 ℃ for 10 minutes, then stirred with a glass rod for 30 minutes, and then the change was measured by the purification process.

As a result, the deoxidation effect of the hydrogen peroxide using sodium hydroxide was increased in proportion to the sodium hydroxide concentration and the amount of the input (FIG. 3). The most effective concentration of deoxidation was 2N, 1%, and about 50% purification efficiency. This is because there is a big difference compared with the deoxidation effect when 1N NaOH 1% is used and it shows that deoxidation is not effective at lower concentrations and doses than 1N NaOH. In addition, when 1N NaOH 3N was used, the difference in deoxidation effect was not significantly different from when 2N NaOH and 1% were added. (Fig. 3). The amount of solids increased in proportion to the amount of sodium hydroxide added. When 1% of 2N sodium hydroxide, which had the highest deoxidation effect, was used, the yield was about 15 ml.

On the other hand, the contents of unsaturated fatty acids and saturated fatty acids were different according to the deoxidation treatment (FIG. 4). The contents of unsaturated fatty acids such as oleic acid (18: 1), linoleic acid (18: 2) and linolenic acid (18: 3) showed the largest reduction rate at 10 ppm sodium hydroxide concentration (Fig. The proportions were 52.5 wt%, 59.4 wt% and 56.8 wt%, respectively, and decreased by about 10 wt% or more compared with palmitic acid (14: 1) and palmitoleic acid (16: 1). However, the content of oleic acid (18: 1) tended to be higher than that of the sodium hydroxide treatment, while the content of other unsaturated fatty acids was decreased at a sodium hydroxide concentration of more than 10 ppm.

C. Test for change in color, polyphenol content and antioxidant activity of purified pepper powder

The acidity, acid value, polyphenol content, antioxidant activity, and fatty acid content of the deacidified and deoxidized acidic colostrum were analyzed (Fig. 6).

As a result, the color difference was drastically lowered than that of crude oil through the defection and deoxidation process. This suggests that the purification has proceeded through the de-oxidation and de-oxidation processes. The content of polyphenols decreased sharply through deionization and deoxidation process. However, antioxidant activity did not change significantly.

Through the above-described defection and deoxidation process, the acidic colostrum can efficiently purify the viscous acidic colostrum without giving great loss to the unsaturated fatty acid and the antioxidative activity.

[Example 2] Experiment on prevention of rancidity by treatment with sodium bicarbonate

A. Saturation test of sodium bicarbonate

30 g of purified colostrum was placed in a 50 ml conical tube and sodium bicarbonate (NaHCO ₃ ) was added at a concentration of 0.5, 1.0, and 2%, and the mixture was incubated at 25 ± 2 ° C for 30 days Respectively.

As a result, the acidity gradually increased when the uncoloured acidic colostrum was placed at a low temperature, while the acid value of the acidic acid added with sodium bicarbonate gradually decreased (FIG. 7). Therefore, it can be seen that sodium bicarbonate is a material delaying the acidosis of Sancho.

Sodium bicarbonate is an abbreviation for sodium bicarbonate, and is a colorless crystal. Solubility in 100 g of water is 8.8 g (15 ° C). The aqueous solution becomes slightly alkaline due to hydrolysis. When heated above 65 ° C, it releases CO ₂ and becomes sodium carbonate. It is most widely used as antacid. It is a white powder and has a distinctive taste. It is weakly alkaline, neutralizes stomach acid in the stomach and generates carbon dioxide gas. By this carbon dioxide gas, the stomach is properly congested and the absorption power is increased, but the stomach is swollen and the trim may come out. Gastric hyperplasia, soreness and vomiting of the mandible, but it has a drawback that it gives an unpleasant bloat on the stomach or secondary gastric acid secretion. This is common to carbonates. It is also used for baking powders, fire extinguishers and the like.

The use of sodium bicarbonate is expected to be very helpful for the storage and distribution of acid oil because it has the advantage of not having any restriction on food circulation and also not having to do it.

Claims (8)

The present invention relates to a method for purifying an acidic colostrum by mixing phosphoric acid,
The acetic acid is further mixed with phosphoric acid in the acidic oil,
The mixture is mixed at a mixing ratio of 99.4 to 99.9% by weight of acidic colostrum, 0.05 to 0.3% by weight of phosphoric acid and 0.05 to 0.3% by weight of acetic acid to remove the proteins and phospholipids as impurities in the fat, (NaOH) to further purify the impurities and extend shelf life,
The sodium hydroxide (NaOH) was mixed at a concentration of 1 to 3 N,
The above defatted and purified unsaturated fatty acid and sodium bicarbonate are further added to the acidic colostrum which is visually improved without damaging the antioxidative activity to delay rancidity,
Wherein said sodium bicarbonate is added at a concentration of 0.5 to 2% by weight of the total weight of said sodium bicarbonate,
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