KR101729733B1 - Method of Producing Rice Beer Using Unsteamed Rice, Steamed Rice and Steamed Barley Mixture - Google Patents
Method of Producing Rice Beer Using Unsteamed Rice, Steamed Rice and Steamed Barley Mixture Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C12/00—Processes specially adapted for making special kinds of beer
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C11/00—Fermentation processes for beer
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- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C11/00—Fermentation processes for beer
- C12C11/02—Pitching yeast
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- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01002—Beta-amylase (3.2.1.2)
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- C12R1/86—
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Abstract
본 발명은 무증자된 쌀, 증자된 쌀 및 증자된 보리를 이용한 쌀맥주의 제조방법에 관한 것으로, 보다 상세하게는 무증자된 쌀에 액화효소제, 당화효소제, 효모 및 물을 첨가하고 1∼3일간 1단 발효시킨 다음, 상기 1단 발효물에 증자된 쌀과 증자된 보리 및 증자된 맥아를 추가로 혼합시키고, 팽화미, 보리, 맥아, 액화효소제, 당화효소제, 효모, 호프 및 물을 첨가한 다음, 1.5∼4.5일간 2단 발효시키는 것을 특징으로 하는 향미와 감미효과가 향상되고 제조기간이 약 5일로 단축된 쌀맥주의 제조방법에 관한 것이다.
본 발명에 따른 방법으로 제조된 쌀맥주는 기존의 제조방법에 비해 쌀맥주 제조시간을 약 5일로 단축시킬 수 있으며, 검품 유지 시간, 알코올 함량, 당도 및 관능 평가 면에서 전반적으로 우수하다.More particularly, the present invention relates to a method for producing rice beer using free rice, roasted rice and roasted barley, and more particularly, to a process for producing rice beer using free rice, roasted rice, yeast and water, After the first stage fermentation for one day, the rice added to the first fermentation product, the added barley and the boiled malt were further mixed, and the rice germ, barley, malt, liquefying enzyme, saccharifying enzyme, yeast, hops and water were added And then fermenting the rice for 1.5 to 4.5 days. The present invention relates to a method for producing rice beer, wherein the flavor and sweetness are improved and the production period is shortened to about 5 days.
The rice beer produced by the method according to the present invention can shorten the production time of rice beer to about 5 days as compared with the conventional production method, and is excellent in overall inspection time, alcohol content, sugar content and sensory evaluation.
Description
본 발명은 무증자된 쌀, 증자된 쌀 및 증자된 보리를 이용한 쌀맥주의 제조방법에 관한 것으로, 보다 상세하게는 무증자된 쌀에 액화효소제, 당화효소제, 효모 및 물을 첨가하고 1∼3일간 1단 발효시킨 다음, 상기 1단 발효물에 증자된 쌀과 증자된 보리 및 증자된 맥아를 추가로 혼합시키고, 팽화미, 보리, 맥아, 액화효소제, 당화효소제, 효모, 호프 및 물을 첨가한 다음, 1.5∼4.5일간 2단 발효시키는 것을 특징으로 하는 향미와 감미효과가 향상되고 제조기간이 약 5일로 단축된 쌀맥주의 제조방법에 관한 것이다.
More particularly, the present invention relates to a method for producing rice beer using free rice, roasted rice and roasted barley, and more particularly, to a process for producing rice beer using free rice, roasted rice, yeast and water, After the first stage fermentation for one day, the rice added to the first fermentation product, the added barley and the boiled malt were further mixed, and the rice germ, barley, malt, liquefying enzyme, saccharifying enzyme, yeast, hops and water were added And then fermenting the rice for 1.5 to 4.5 days. The present invention relates to a method for producing rice beer, wherein the flavor and sweetness are improved and the production period is shortened to about 5 days.
동양에서 술은 전통적으로 혼성주를 제외하고는 주로 전분질 원료인 곡류원료에 예로부터 전래되는 누룩을 발효제로 사용하거나, 누룩 대신 근래 개발된 개량형 발효제인 입국을 사용하여 빚은 곡주(탁주, 약주, 청주 등)를 그대로 음용하거나 이를 증류하여 증류주(증류식소주, 희석식소주)로 음용하는 경우가 대부분이다. Traditionally, sake in oriental sake has traditionally been used for fermentation of yeast, which has been traditionally used for cereal raw materials, which is a raw material for starch, as a fermentation agent, or as an improved fermentation agent developed recently instead of yeast, ) As it is, or it is distilled, and it is mostly consumed as distillation stock (distillation type shochu, dilution shochu).
서양의 술 역시 발효주와 증류주로 대별할 수 있으며, 발효주로는 포도 등을 원료로 효모만으로 발효시키는 과실주와 발아곡류(보리맥아, 밀맥아)를 원료로 당화후 효모만으로 발효시키는 맥주를 들 수 있고, 증류주로는 포도주를 증류한 브랜디와 맥주를 증류한 위스키를 들 수 있으나, 동양과 다른점은 누룩 등 국을 전혀 사용하지 않고 주류를 제조한다는 점에서 큰 차이가 있다고 할 수 있다.Western wines can also be divided into fermented wine and distilled wine. Fermented wines are fermented by fermenting only yeast with raw materials such as grapes, and beer fermented with yeast only after saccharification with germinated cereals (barley malt, wheat malt) , Distilled wine brandy distilled wine and whiskey distilled beer. However, the difference between the Oriental and soup, such as the country does not use any liquor production is a big difference can be said.
즉, 서양의 발효주는 발효제를 사용하지 않는 대신 원료중의 발효성 당을 그대로 이용하거나 곡류(주로 맥아)의 발아시 생성되는 효소에 의해 당화가 이루어 질 수 있도록 함으로써 주질에 있어서는 깨끗하고 담백하며, 원료고유의 맛과 향을 살릴 수 있는 반면, 동양의 발효주는 누룩, 입국, 조효소제 등 발효제를 사용함으로써 발효제 자체에 함유되어 있는 야생곰팡이류, 야생효모류 등 각종 미생물에 의한 감미, 산미, 고미 등 다양하고 풍부한 맛과 향을 반영시킨 우수한 주질의 발효주라고 할 수 있다.In other words, Western fermented beverages do not use fermentation agents but instead use fermentable sugars in raw materials as they are, or they can be saccharified by the enzymes generated during the germination of cereals (mainly malt), so they are clean, While the taste and flavor of raw materials can be saved, the oriental fermented beverages have various kinds of fermented products such as yeast, entry, coenzyme, etc., and various kinds of microorganisms such as wild fungi and wild yeast And it is an excellent quality fermented wine that reflects rich taste and aroma.
동양의 전통적인 방법으로 쌀 또는 보리를 원료로 하여 주류를 제조할 경우, 침미·증자 등의 원료처리 후 누룩, 입국을 거쳐 효모를 첨가하여 발효시켜 탁주 또는 약주형태의 발효주를 제조하는 것이 일반적이며, 이와 같은 방법에 의해 제조된 주류는 누룩 등에서 유래하는 곰팡이향이 강하므로 신세대 젊은층으로부터 외면을 받아 그 수요가 줄고 있는 추세이다.In the case of producing a mainstream using rice or barley as a raw material in accordance with the traditional method of Oriental tradition, it is general to produce yeast or fermented soybean wine by fermenting yeast after adding raw yeast, The mainstream produced by this method has a strong mold flavor derived from yeast and the like, and thus the demand from the younger generation is getting smaller.
현재 국내의 젊은층에서 널리 음용되고 있는 맥주는 서양에서 전통적으로 제조되고 있는 술로써, 통상적으로, 보리 또는 밀을 발아시키고 발아 시 생성된 당화효소를 비롯한 각종 효소에 의해 보리 또는 밀속의 전분을 당화시켜 당분으로 전환시킨 다음 호프를 첨가하고 자비시켜 당화액을 제조한 후, 맥주효모의 작용에 의해 알코올이 생성되며, 숙성중에 탄산가스를 잔류시키는 단행복발효방식에 의해 제조하고 있다.Currently, beer widely consumed in domestic young people is a wine that is traditionally produced in Western countries. It is usually made by germinating barley or wheat and saccharifying starch in barley or wheat by various enzymes including glycosylation enzyme produced during germination The fermentation broth is produced by a simple fermentation method in which alcohol is produced by the action of brewer's yeast and carbonic acid gas is retained during fermentation.
최근 다양한 특징을 가진 맥주의 수입 증대 등 주류 사이클의 변화로 국산 쌀 소비를 이끌어갈 주류 제품 개발 필요성이 제기되고 있으며, 맥주 제조에 사용되는 거의 모든 원료는 수입 원료로 국산화가 시급하다.Recent changes in the mainstream cycle, such as increasing imports of beer with various characteristics, have raised the need to develop mainstream products that will lead to domestic rice consumption. Almost all raw materials used for beer production are urgently needed to be imported.
관련 종래기술을 보면 한국특허등록 제10-0651266호(생벼 또는 백미를 이용한 순쌀맥주의 제조방법)는 생벼 또는 백미를 원료로 하여 종래 맥주와 동일하게 발효시킨 저산미형 순쌀맥주의 제조방법(약 19일 소요)을 개시하고 있으며, 이 기술은 보리맥아를 포함하지 않기 때문에 고유의 맥주 풍미가 가미되지 못한 문제가 있다. 또한, 한국특허등록 제10-0978203호(쌀과 보리를 기재로 한 홍주의 제조방법 및 그로부터 제조되는 홍주)는 쌀 및 보리를 이용하여 탁주 및 약주의 제조방법(약 8일 소요)을 제공하고 있으나, 이는 맥주의 제조방법과는 차이가 있다. 아울러, 한국특허등록 제10-0814661호(쌀과 과일을 이용한 과일쌀맥주의 제조방법)는 주원료로 보리 대신에 쌀분말 및 천연과일즙을 첨가하여 제조된 과일쌀맥주의 제조방법(약 15일 소요)을 개시하고 있다. 상기와 같이 쌀맥주를 제조하는 데 있어서 사용원료, 제조방법, 시설규모 등에서 서로 매우 다르기 때문에, 제조방법에 따라 쌀맥주의 품질은 차이가 있게 된다.Korean Patent Registration No. 10-0651266 (method of producing pure rice beer using raw rice or white rice) is a method of producing a hypoxic pure rice beer fermented in the same manner as conventional beer, using raw rice or white rice as a raw material And the technology does not include barley malt, so there is a problem that the inherent beer flavor is not added. In addition, Korean Patent Registration No. 10-0978203 (a method of producing red flakes based on rice and barley, and Hongju manufactured therefrom) provides a method of producing Takju and Yakju using rice and barley (takes about 8 days) , Which is different from the beer manufacturing method. In addition, Korean Patent Registration No. 10-0814661 (a method for producing fruit rice beer using rice and fruit) is a method for manufacturing a fruit rice beer prepared by adding rice powder and natural fruit juice instead of barley as a main raw material (about 15 days Required). As described above, the quality of the rice beer is different according to the manufacturing method because the raw materials, the manufacturing method, and the scale of the facilities are very different from each other in manufacturing the rice beer.
이에, 본 발명자들은 풍미가 증진된 쌀맥주를 제조하기 위해 예의 노력한 결과, 동양의 발효주 제조기법과 서양의 발효주 제조기법을 접목시켜, 1단 및 2단 발효 단계로 구성된 쌀맥주 제조과정에 무증자된 쌀, 증자된 쌀 및 증자된 보리 혼합물을 최적조건으로 첨가하여 약 5일간 쌀맥주를 제조할 경우, 쌀맥주의 향미 및 감미효과가 기존방법으로 제조된 맥주에 비해 증진된 것을 확인하고, 본 발명을 완성하게 되었다.
As a result of intensive efforts to produce rice beer with enhanced flavor, the inventors of the present invention have found that, by combining the technique of producing oriental fermented beans with the technique of producing fermented beans in western countries, It was confirmed that the flavor and sweetness of rice beer were improved compared with beer prepared by conventional methods when rice beer was prepared for about 5 days by adding optimum amount of rice, Thereby completing the invention.
본 발명의 목적은 무증자된 쌀, 증자된 쌀 및 증자된 보리 혼합물에 맥주효모를 첨가하여 알코올 함량이 적당하게 생성되도록 약 5일간 1단 및 2단 발효시키는 것을 특징으로 하는 쌀맥주의 향미와 감미효과가 증진된 쌀맥주의 제조방법을 제공하는데 있다.
The object of the present invention is to provide a fermented rice beer which is characterized by adding brewer's yeast to the roasted rice, the roasted rice and the roasted barley mixture to ferment the roasted rice for about 5 days to produce an appropriate alcohol content. And to provide a method for producing a rice beer in which the sweetness effect is enhanced.
상기 목적을 달성하기 위하여 본 발명은, (a) 무증자된 쌀에 액화효소제, 당화효소제, 효모 및 물을 첨가한 다음, 15∼25℃에서 1∼3일간 발효시키는 1단 발효 단계; (b) 상기 (a) 단계에서 준비된 1단 발효물에 증자된 쌀과 증자된 보리 및 증자된 맥아를 추가로 혼합시키고, 팽화미, 보리, 맥아, 액화효소제, 당화효소제, 효모 및 물을 첨가한 다음, 15∼25℃에서 0.5∼1.5일간 발효시킨 후 호프를 첨가하고, 15∼25℃에서 1∼3일간 발효시키는 2단 발효 단계; 및 (c) 상기 (b) 단계에서 수득한 2단 발효물을 여과하여 쌀맥주로 수득하는 단계를 포함하는 무증자된 쌀, 증자된 쌀 및 증자된 보리의 혼합물을 이용한 쌀 맥주의 제조방법을 제공한다.
In order to accomplish the above object, the present invention provides a method for producing fermented rice, comprising the steps of: (a) a one-stage fermentation step of adding a liquefying enzyme, a saccharifying enzyme, yeast and water to rice without sugar, and fermenting the mixture at 15 to 25 ° C for 1 to 3 days; (b) adding the rice added to the first stage fermented product prepared in the step (a) above, the added barley and the boiled malt, and adding the boiled rice, barley, malt, liquefying enzyme, saccharifying enzyme, yeast and water Followed by fermentation at 15 to 25 ° C for 0.5 to 1.5 days followed by addition of a hop and fermentation at 15 to 25 ° C for 1 to 3 days; And (c) filtering the two-stage fermentation product obtained in the step (b) to obtain a rice beer, which comprises the steps of: (a) preparing a rice beer comprising a mixture of rice bran, rice bran, to provide.
본 발명에 따른 방법으로 제조된 쌀맥주는 기존의 제조방법에 비해 쌀맥주 제조시간을 약 5일로 단축시킬 수 있으며, 검품 유지 시간, 알코올 함량, 당도 및 관능 평가 면에서 전반적으로 우수하다.
The rice beer produced by the method according to the present invention can shorten the production time of rice beer to about 5 days as compared with the conventional production method, and is excellent in overall inspection time, alcohol content, sugar content and sensory evaluation.
도 1은 쌀 및 보리 첨가량에 따른 맥주의 알코올 함량(%)을 나타낸 것이다.
도 2는 쌀 및 보리 첨가량에 따른 맥주의 감각 평가(감미 효과)를 나타낸 것이다.
도 3은 증자된 쌀, 무증자된 쌀 및 보리 첨가량에 따른 맥주의 알코올 함량(%)을 나타낸 것이다.
도 4는 맥아 종류에 따른 맥주의 감각 평가(감미 효과)를 나타낸 것이다.
도 5는 호프 종류에 따른 맥주의 감각 평가(감미 효과)를 나타낸 것이다.
도 6은 호프 첨가량에 따른 맥주의 감각 평가(감미 효과)를 나타낸 것이다.
도 7은 맥주 효모 종류에 따른 맥주의 알코올 함량(%)을 나타낸 것이다
도 8은 맥주 효모 종류에 따른 맥주의 감각 평가(감미 효과)를 나타낸 것이다.
도 9는 흑맥아 첨가량에 따른 맥주의 알코올 함량(%)을 나타낸 것이다
도 10은 흑맥아 첨가량에 따른 맥주의 감각 평가(감미 효과)를 나타낸 것이다.Figure 1 shows the alcohol content (%) of beer according to the addition of rice and barley.
2 shows the sensory evaluation (sweetness effect) of beer according to the addition amount of rice and barley.
Figure 3 shows the alcohol content (%) of beer according to the amount of rice added, rice added without sugar addition, and barley added.
Fig. 4 shows the sensory evaluation (sweetness effect) of beer according to malt varieties.
Figure 5 shows the sensory evaluation (sweetness effect) of beer according to the type of hops.
Fig. 6 shows the sensory evaluation (sweetness effect) of beer according to the addition amount of the hops.
Figure 7 shows the alcohol content (%) of beer according to the type of beer yeast
Figure 8 shows the sensory evaluation (sweetness effect) of beer according to the type of beer yeast.
Figure 9 shows the alcohol content (%) of beer according to the amount of malt malt added
Fig. 10 shows the sensory evaluation (sweetness effect) of beer according to the amount of black malt added.
본 발명에서 증자된 쌀, 무증자된 쌀 및 증자된 보리 혼합물은 통상적인 방법에 따라 제조하였다. 특히 무증자된 쌀은 증자시키지 않고 발효시키는 것을 특징으로 한다. 이와 같이 증자과정을 생략함으로써 증미(증자된 쌀)를 식히는 단계에서 발생할 수 있는 잡균의 오염 가능성 및 영양분의 감소를 막을 수 있다.The rice, the roasted rice and the roasted barley mixture, which have been added in the present invention, were prepared according to a conventional method. In particular, rice without sugar is fermented without adding sugar. By omitting the capital increase process, it is possible to prevent possible contamination of germs and reduction of nutrients that may occur in the step of cooling the increase (rice added).
본 발명은 일 관점에서, (a) 무증자된 쌀에 액화효소제, 당화효소제, 효모 및 물을 첨가한 다음, 15∼25℃에서 1∼3일간 발효시키는 1단 발효 단계; (b) 상기 (a) 단계에서 준비된 1단 발효물에 증자된 쌀과 증자된 보리 및 증자된 맥아를 추가로 혼합시키고, 팽화미, 보리, 맥아, 액화효소제, 당화효소제, 효모 및 물을 첨가한 다음, 15∼25℃에서 0.5∼1.5일간 발효시킨 후 호프를 첨가하고, 15∼25℃에서 1∼3일간 발효시키는 2단 발효 단계; 및 (c) 상기 (b) 단계에서 수득한 2단 발효물을 여과하여 쌀맥주로 수득하는 단계를 포함하는 무증자된 쌀, 증자된 쌀 및 증자된 보리의 혼합물을 이용한 쌀맥주의 제조방법에 관한 것이다.In one aspect, the present invention provides a method for producing fermented rice, comprising the steps of: (a) a one-stage fermentation step of adding a liquefying enzyme, a saccharifying enzyme, yeast, and water to rice without sugar, and fermenting at 15 to 25 ° C for 1 to 3 days; (b) adding the rice added to the first stage fermented product prepared in the step (a) above, the added barley and the boiled malt, and adding the boiled rice, barley, malt, liquefying enzyme, saccharifying enzyme, yeast and water Followed by fermentation at 15 to 25 ° C for 0.5 to 1.5 days followed by addition of a hop and fermentation at 15 to 25 ° C for 1 to 3 days; And (c) filtering the two-stage fermentation product obtained in the step (b) to obtain a rice beer, which comprises the step of: .
본 발명에 있어서, 상기 무증자된 쌀은 쌀을 1∼3시간 동안 물에 침지하고, 분쇄시킨 쌀인 것을 특징으로 할 수 있으며, 상기 증자된 쌀은 쌀을 1∼3시간 동안 물에 침지하고, 물빼기를 한 다음, 95∼100℃에서 15∼45분간 찐 고두밥인 것을 특징으로 할 수 있고, 상기 증자된 보리 및 증자된 맥아는 보리 및 맥아를 1.5∼4.5시간 동안 물에 침지하고, 물빼기를 한 다음, 95∼100℃에서 15∼45분간 찐 고두밥인 것을 특징으로 할 수 있다.In the present invention, the rice-free rice is characterized in that the rice is rice in which the rice is immersed in water for 1 to 3 hours and pulverized, wherein the rice is immersed in water for 1 to 3 hours, Which is steamed at 95 to 100 DEG C for 15 to 45 minutes after the water is drained, wherein the above-mentioned barley and the boiled malt are obtained by immersing barley and malt in water for 1.5 to 4.5 hours, And then steamed at 95 to 100 DEG C for 15 to 45 minutes.
본 발명에 있어서, 상기 액화효소제는 바실러스(Bacillus sp .) 유래의 α-아밀라아제인 것을 특징으로 할 수 있으며, 상기 당화효소제는 아스퍼질러스나이거(Aspergillus niger) 유래의 β-아밀라아제인 것을 특징으로 할 수 있으며, 상기 (b) 단계에서 1단 발효물에 추가로 혼합된 쌀과 보리의 함량비는 45∼55 중량%:55∼45 중량%인 것을 특징으로 할 수 있고, 상기 효모는 사카로마이세스 세레비지에(Saccharomyces cerevisiae) 또는 사카로마이세스 칼스버겐시스(Saccharomyces carlsbergensis)인 것을 특징으로 할 수 있다.In the present invention, the liquefying enzyme is Bacillus sp . Amylase derived from Aspergillus oryzae, and the saccharifying enzyme is Aspergillus or Aspergillus amylase derived from niger , and the content ratio of rice and barley further mixed in the first stage fermented product in step (b) is 45-55 wt%: 55-45 wt% The yeast may be characterized by Saccharomyces cerevisiae S. cerevisiae or Saccharomyces carlsbergensis .
본 발명의 일 실시예에서는, 증자된 쌀 및 증자된 보리 첨가량에 따른 쌀맥주의 품질 특성을 분석하였다. 그 결과, 도 1, 도 2 및 표 2에 나타난 바와 같이, 시험예 1의 증자된 쌀 55 중량% 및 증자된 보리 45 중량%(알코올 9.9%)가 첨가된 혼합물로 2단 발효시켜 제조된 쌀맥주의 관능이 가장 우수한 것으로 나타났다. In one embodiment of the present invention, the quality characteristics of rice beer according to the amount of the added rice and the added barley were analyzed. As a result, as shown in Fig. 1, Fig. 2 and Table 2, the rice prepared by the two-stage fermentation with the mixture in which 55 wt% of the roasted rice of Test Example 1 and 45 wt% of the increased roasted barley (9.9% The beer was found to have the best sensuality.
본 발명의 다른 실시예에서는, 1단 발효 및 2단 발효에 이용되는 무증자된 쌀 및/또는 증자된 쌀을 다양한 조합으로 증자된 보리와 혼합하고 발효시켜 쌀맥주를 제조한 다음, 상기 쌀맥주의 품질 평가 및 관능 평가를 수행하였다. 그 결과, 표 3에 나타난 바와 같이, 실시예 1의 2단 발효에서 증자된 쌀 55 중량%(알코올 8.4%)로 제조된 쌀맥주의 관능이 가장 우수하게 나타났다. In another embodiment of the present invention, a rice beer is prepared by mixing and adding fermented rice and / or roasted rice used in one-stage fermentation and two-stage fermentation to a variety of combinations of barley added in various combinations, Were evaluated for their quality and sensory properties. As a result, as shown in Table 3, the rice beer made from 55 wt% (alcohol 8.4%) of rice added in the two-stage fermentation of Example 1 showed the best sensation.
또한, 표 4에 나타난 바와 같이, 실시예 1의 무증자된 쌀로 1단 발효하고, 증자된 쌀로 2단 발효하여 제조된 쌀맥주의 거품 유지 시간이 45초로 나타났고, 이는 비교예 1∼5의 쌀맥주 및 비교예 6의 맥주의 거품 유지 시간인 20∼40초보다 높게 나타났음을 확인할 수 있었다. 또한, 실시예 1의 쌀맥주 알코올 함량이 8.34%로 나타나 알코올 함량 4.3∼6.17%인 비교예 1∼6의 맥주보다 높게 나타났다.As shown in Table 4, the bubble retention time of the rice beer produced by the two-stage fermentation with the rice grown in the first stage fermented with the rice roasted with the rice roasted in Example 1 was 45 seconds, The beer of rice beer and the beer of Comparative Example 6 was higher than the bubble retention time of 20 to 40 seconds. In addition, the rice beer alcohol content of Example 1 was 8.34%, which was higher than that of Comparative Examples 1 to 6 where the alcohol content was 4.3 to 6.17%.
아울러, 쌀맥주의 관능 평가를 수행한 결과, 표 5에 나타난 바와 같이, 실시예 1의 쌀맥주의 관능 평가 수치가 비교예 1∼6의 맥주보다 높게 나타났음을 확인할 수 있었다.As a result of sensory evaluation of the rice beer, it was confirmed that the sensory evaluation value of the rice beer of Example 1 was higher than that of the beer of Comparative Examples 1 to 6, as shown in Table 5.
결국, 본 발명의 1단 발효 및 2단 발효로 무증자된 쌀 및 증자된 쌀을 다양한 조합으로 이용하여 맥주를 제조할 경우, 기존방법(소요시간: 15일 이상)에 비해 제조시간(5∼8일)이 짧고, 상기 방법으로 제조된 쌀맥주는 검품 유지 시간, 알코올 함량, 당도 및 관능 평가 면에서 기존 맥주보다 전반적으로 우수함을 확인할 수 있었다. 특히 무증자된 쌀로 1단 발효시킨 다음, 증자된 쌀 55 중량%와 증자된 보리 45 중량%을 혼합하여 2단 발효시켜 제조된 쌀맥주의 관능이 가장 우수하게 나타났다.As a result, when the beer is produced using various combinations of the rice grown in the first stage fermentation and the second stage fermentation in the present invention, the production time (5 ~ 8 days) was short, and it was confirmed that rice beer produced by the above method was generally superior to conventional beer in terms of inspection time, alcohol content, sugar content and sensory evaluation. In particular, the rice beer fermented by two stages of a mixture of 55 wt% of the increased rice and 45 wt% of the increased rice showed the highest sensory value.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 예시하기 위한 것으로서, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지 않는 것은 당업계에서 통상의 지식을 가진 자에 있어서 자명할 것이다.
Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these examples are for illustrative purposes only and that the scope of the present invention is not construed as being limited by these examples.
실시예Example 1: One: 쌀맥주의Rice beer 제조 Produce
다음과 같은 방법으로 쌀맥주를 제조하였다.Rice beer was prepared as follows.
먼저, 무증자된 쌀, 증자된 쌀 및 증자된 보리를 하기와 같이 준비하였다((1)∼(3)).First, rice without sugar, rice added and rice bran added were prepared as follows ((1) to (3)).
(1) 무증자된 쌀은 약 2시간 동안 물에 침지하고, 약 1시간 동안 물빼기를 한 다음, 룰러에 분쇄시켜 제조하였다.(1) The free rice was prepared by immersing in water for about 2 hours, draining for about 1 hour, and grinding in a ruler.
(2) 증자된 쌀은 약 2시간 동안 물에 침지하고, 약 1시간 동안 물빼기를 한 다음, 찜기를 통해 95∼100℃에서 약 30분간 쪄서 고두밥을 제조하였다.(2) The roasted rice was immersed in water for about 2 hours, drained for about 1 hour, and steamed at 95 to 100 ° C for about 30 minutes through a steamer to produce a rice cake.
(3) 증자된 보리 및 증자된 맥아는 약 3시간 동안 물에 침지하고, 약 1시간 동안 물빼기를 한 다음, 찜기를 통해 95∼100℃에서 약 30분간 쪄서 고두밥을 제조하였다.(3) The roasted barley and the boiled malt were immersed in water for about 3 hours, drained for about 1 hour, and steamed at 95 to 100 ° C for about 30 minutes through a steamer to prepare a rice cake.
상기 준비된 무증자된 쌀, 증자된 쌀 및 증자된 보리를 하기((i) 및 (ii))와 같이 필요로 하는 첨가물을 첨가한 다음, 이를 1 단 발효 또는 2단 발효시켰다(표 1). The prepared roasted rice, the roasted rice and the roasted barley were added to the required additives such as (i) and (ii) and then subjected to one-stage fermentation or two-stage fermentation (Table 1).
(i) 1단 발효: 무증자된 쌀에 정제효소, 효모 및 물이 첨가된 혼합물을 준비한 다음, 20℃에서 약 2일간 발효시켰다. 필요에 따라서 1단 발효는 무증자된 쌀 대신 증자된 쌀을 이용하여 발효시키거나, 무증자된 쌀 및 증자된 쌀을 혼합하여 발효시키는 1단 발효 단계로 구성될 수 있었다. (i) Single Stage Fermentation : A mixture containing purified enzyme, yeast and water was added to the rice without sugar, and then fermented at 20 ° C for about 2 days. As needed, the first stage fermentation could consist of a single stage fermentation step in which fermented rice was used instead of free rice, or fermented with mixed rice without added rice and added rice.
(ii) 2단 발효: 1단 발효시킨 발효물에 증자된 쌀, 증자된 보리 및 증자된 맥아를 혼합시키고, 팽화미, 보리, 맥아, 정제효소, 효모, 호프 및 물이 첨가된 혼합물을 준비한 다음, 20℃에서 약 3일간 발효시켰다. 상기 호프(hop)(사츠(sazz), 캐스캐이드(cascade), 고수(coriander), 너겟(nugget) 또는 이들의 조합으로 구성된 호프)는 2단 발효 2일차 발효물에 첨가하였다. 필요에 따라서 2단 발효는 증자된 쌀 대신 무증자된 쌀을 이용하여 발효시키거나, 무증자된 쌀 및 증자된 쌀을 혼합하여 발효시키는 2단 발효 단계로 구성될 수 있었다. (ii) Two-Stage Fermentation : A mixture of rice, roasted barley and boiled malt added to the fermented product of the first stage fermented product, and a mixture prepared by adding boiled rice, barley, malt, purified enzyme, yeast, Then, it was fermented at 20 DEG C for about 3 days. The hops (hops consisting of sazz, cascade, coriander, nugget or a combination thereof) were added to the second stage fermentation secondary fermentations. If necessary, the two - stage fermentation could consist of two stages of fermentation, in which the rice was fermented using free rice instead of free rice, or fermented with mixed rice and free rice.
상기 정제효소는 액화효소제 및 당화효소제가 혼합된 것이다. 상기 액화효소제는 바실러스(Bacillus sp .) 유래의 α-아밀라아제이고, 당화효소제는 아스퍼질러스나이거(Aspergillus niger) 유래의 β-아밀라아제이다.The purified enzyme is a mixture of a liquefying enzyme and a saccharifying enzyme. The liquefying enzyme may be selected from the group consisting of Bacillus sp . Amylase, and the saccharifying enzyme is Aspergillus or Aspergillus niger- derived? -amylase.
상기 효모는 사카로마이세스 세레비지에(Saccharomyces cerevisiae)가 함유된 Safale S-04, Safale US-05, Safbrew WB-06, Saflager S-23, Safbrew S-33, Saflager W-34/70 또는 Safbrew T-58을 이용하였다. The yeast may be selected from the group consisting of Safale S-04, Safale US-05, Safbrew WB-06, Saflager S-23, Safbrew S-33, Saflager W-34/70 or Safbrew S-04 containing Saccharomyces cerevisiae . T-58 was used.
상기 쌀은 추청벼를 이용하였고, 상기 맥아는 0.7%, 1.2% 또는 1.6%(w/w) 맥아(필스너(Pilsner), 페일(Pale), 블랙 패턴트(Black patent) 또는/및 흑맥아(Black malt))를 첨가하여 발효과정에 이용하였다. The rice was chrysanthemum and the malt was found to contain 0.7%, 1.2% or 1.6% (w / w) malt (Pilsner, Pale, Black patent and / or Black malt malt) was added and used for the fermentation process.
상기 쌀맥주 제조방법을 조건화하는 과정에서 이용된 쌀과 보리 혼합물의 쌀 대비 보리의 혼합비율을 28∼55 중량%:72∼45 중량%로 하였고, 무증자된 쌀과 증자된 쌀 혼합물의 무증자된 쌀 대비 증자된 쌀의 혼합비율을 40∼60 중량%:60∼40 중량%로 혼합하여 쌀맥주를 제조하였다.
The mixing ratio of the rice to the barley mixture used in the process of conditioning the rice beer was set to 28 to 55 wt%: 72 to 45 wt%, and the mixture of the rice without added rice and the rice mixture Rice beer was prepared by mixing 40 to 60% by weight of 60 to 40% by weight of the mixture of rice added to the rice.
실시예Example 2: 증자된 쌀 및 증자된 보리 첨가량에 따른 쌀맥주의 품질 특성 2: Quality characteristics of rice beer according to the amount of added rice and added barley
표 1과 같이 시험예 1∼4는 정해진 비율(%)로 쌀 및 보리 혼합물을 배합한 다음, 실시예 1의 방법으로 약 5일간 발효시켰다. 그 다음, 시험예 1∼4로부터 수득한 발효물을 여과하여 쌀맥주로 수득하였다. 상기 쌀맥주의 알코올 함량(%)은 비중계를 이용하여 1일 간격으로 측정하였다. 또한, 상기 쌀맥주에 대한 관능 평가는 35명의 일반 직장인으로 구성된 평가단을 통하여 외관(색감), 향미, 전체적인 맛, 바디감 및 종합적 평가에 대하여 9점 척도법으로 측정하였다.
As shown in Table 1, in Test Examples 1 to 4, the rice and the barley mixture were mixed at a predetermined ratio (%), and then fermented by the method of Example 1 for about 5 days. Then, the fermented product obtained from Test Examples 1 to 4 was filtered to obtain a rice beer. The alcohol content (%) of the rice beer was measured at intervals of one day using a specific gravity meter. The sensory evaluation of the rice beer was performed by a nine-point scale method for appearance (color), flavor, overall taste, body sensation, and comprehensive evaluation through an evaluation team composed of 35 ordinary workers.
하기 표 1은 2단 발효에서 이용된 증자된 쌀 및 증자된 보리 혼합물의 혼합률을 나타낸 것이다.Table 1 below shows the mixing ratios of the roasted rice and the boiled barley mixture used in the two-stage fermentation.
하기 표 2는 2단 발효에서 증자된 쌀 및 증자된 보리 첨가량에 따른 쌀맥주의 감각 평가(감미 효과)를 나타낸 것이다(도 2).Table 2 shows the sensory evaluation (sweetness effect) of rice beer according to the amount of rice added and the amount of added rice added in the two-stage fermentation (Fig. 2).
그 결과, 도 1, 도 2 및 표 2에 나타난 바와 같이, 실시예 1의 증자된 쌀 55 중량% 및 증자된 보리 45 중량%(알코올 9.9%)가 첨가된 혼합물로 2단 발효시켜 제조된 쌀맥주의 관능이 가장 우수한 것으로 나타났다.
As a result, as shown in Fig. 1, Fig. 2 and Table 2, the rice prepared by the two-stage fermentation with the mixture containing 55 wt% of the roasted rice of Example 1 and 45 wt% of the increased roasted rice (9.9% The beer was found to have the best sensuality.
실시예Example 3: 3: 무증자된Cashless 쌀, 증자된 쌀 및 증자된 보리 첨가량에 따른 Depending on the amount of rice, added rice and added barley 쌀맥주의Rice beer 품질 특성 Quality characteristics
실시예 1의 쌀맥주 제조방법에 있어서, 1단 발효물 및 2단 발효물의 곡물 조성에 따른 쌀맥주의 품질을 비교하기 위해, 표 3∼5에 나타난 바와 같이, 1단 발효 및 2단 발효에 이용되는 무증자된 쌀 및/또는 증자된 쌀을 다양한 조합으로 혼합하고, 실시예 2의 결과를 토대로 쌀 및 보리의 혼합비율이 55 중량%:45 중량%가 되도록 배합한 다음, 약 5일간 발효(2단 발효조건에서 약 3일간 추가로 발효 가능)시키고, 실시예 1 및 비교예들로부터 수득한 발효물을 여과하여 쌀맥주로 수득하였다. In order to compare the quality of rice beer according to the grain composition of the first stage fermentation product and the second stage fermentation product in the rice beer manufacturing method of Example 1, as shown in Tables 3 to 5, in the first stage fermentation and the second stage fermentation The rice and barley were mixed so as to have a mixing ratio of 55% by weight to 45% by weight based on the result of Example 2, and then fermented for about 5 days (Further fermentation was possible for about 3 days under two-stage fermentation conditions), and the fermented product obtained from Example 1 and Comparative Examples was filtered to obtain a rice beer.
표 4 및 표 5의 비교예 6은 당업계의 통상적인 방법으로 제조된 맥주로 약 15일간 발효시킨 다음, 발효물을 여과하여 맥주로 수득하였다. 상기 수득한 맥주에 대해 품질 평가 및 관능 평가를 수행하였다.Comparative Example 6 of Tables 4 and 5 was fermented with beer prepared by a conventional method in the art for about 15 days, and then the fermented product was filtered to obtain a beer. The beer thus obtained was subjected to quality evaluation and sensory evaluation.
실시예 1 및 비교예들의 맥주(들)의 품질 평가는 하기와 같이 수행되었다.The quality evaluation of the beer (s) of Example 1 and comparative examples was performed as follows.
(1) 거품 유지 시간(초): 최종 수득한 맥주의 거품 유지 시간을 측정하였다.(1) Foam retention time (seconds): The bubble retention time of the final obtained beer was measured.
(2) 알코올 함량(%): 맥주의 알코올 함량을 비중계를 사용하여 1일 간격으로 측정하였다. (2) Alcohol content (%): The alcohol content of beer was measured by using a densitometer at intervals of one day.
(3) 당도(brix): 맥주의 당도는 당도계(KEM, RA-250WE, 일본)를 사용하여 실온에서 3회 반복하여 측정하였다.(3) Brix: The sugar content of beer was measured three times at room temperature using a sugar meter (KEM, RA-250WE, Japan).
(4) 산도(ml): 맥주 내 총산을 측정하기 위해 시료(맥주) 100ml를 취해 페놀프탈레인 지시약과 BTB-NR 지시약을 몇 방울 가하고 중화되는데 소비되는 0.1N NaOH의 용액의 용량(ml)을 총 산도로 3회 반복하여 측정하였다(국세청 기술연구소의 주류제조 교본의 주류분석교본 참조).(4) Acidity (ml): To measure total acid in beer, take 100 ml of sample (beer), add a few drops of phenolphthalein indicator and BTB-NR indicator, and measure the volume (ml) of 0.1N NaOH solution (See the mainstream analysis manual of the mainstream manufacturing manual of the National Institute of Advanced Industrial Science and Technology).
아울러, 상기 맥주(들)에 대한 관능 평가는 35명의 일반 직장인으로 구성된 평가단을 통하여 외관(색감), 향미, 전체적인 맛, 바디감 및 종합적 평가에 대하여 9점 척도법으로 측정하였다.
In addition, the sensory evaluation of the beer (s) was carried out by a nine-point scale method for appearance (color), flavor, overall taste, body sensation and comprehensive evaluation through an evaluation team composed of 35 general workers.
하기 표 3은 1단 발효 및 2단 발효에서 이용된 무증자된 쌀 및/또는 증자된 쌀 및 증자된 보리 혼합물 첨가량에 따른 쌀맥주의 감각 평가(감미 효과)를 나타낸 것이다.The following Table 3 shows the sensory evaluation (sweetness effect) of rice beer according to the addition amount of roasted rice and / or roasted rice and roasted barley mixture used in single stage fermentation and two stage fermentation.
하기 표 4는 1단 발효 및 2단 발효에서 이용된 무증자된 쌀 및/또는 증자된 쌀 및 증자된 보리 혼합물의 발효에 따른 쌀맥주의 알코올 함량, 당도, 산도 및 거품 유지 시간을 나타낸 것이다. Table 4 below shows the alcohol content, sugar content, acidity and bubble retention time of rice beer fermentation of the roasted rice and / or roasted rice and the roasted barley mixture used in the first stage fermentation and the second stage fermentation.
하기 표 5는 1단 발효 및 2단 발효에서 이용된 무증자된 쌀 및/또는 증자된 쌀 및 증자된 보리 혼합물의 발효에 따른 쌀맥주의 감각 평가(감미 효과)를 나타낸 것이다The following Table 5 shows the sensory evaluation (sweetening effect) of rice beer following fermentation of roasted rice and / or roasted rice and roasted barley mixture used in single stage fermentation and two stage fermentation
그 결과, 표 3에 나타난 바와 같이, 실시예 1의 2단 발효에서 증자된 쌀 55 중량%(알코올 8.4%)로 제조된 쌀맥주의 관능이 가장 우수하게 나타났다. 따라서, 실시예 1의 방법으로 약 5일 만에 적절한 알코올 함량과 관능이 우수한 생맥주를 제조할 수 있었고, 이는 숙성단계를 포함하더라도 약 10일 만에 제조할 수 있어, 17∼36일이 소요되는 기존의 제조방법에 비해 현저하게 쌀맥주의 제조시간을 단축함에도 불구하고 우수한 품질의 쌀맥주를 제조하였다.As a result, as shown in Table 3, the rice beer made from 55 wt% (alcohol 8.4%) of rice added in the two-stage fermentation of Example 1 showed the best sensation. Therefore, in the method of Example 1, a draft beer having an appropriate alcohol content and excellent functionality was able to be produced in about 5 days, and it can be produced in about 10 days even though the aging step is included, and it takes 17 to 36 days Rice beer of high quality was produced despite remarkably shortening the manufacturing time of rice beer compared with the conventional manufacturing method.
또한, 표 4에 나타난 바와 같이, 실시예 1의 무증자된 쌀로 1단 발효하고, 증자된 쌀로 2단 발효하여 제조된 쌀맥주의 거품 유지 시간이 45초로 나타났고, 이는 비교예 1∼5의 쌀맥주 및 비교예 6의 맥주의 거품 유지 시간인 20∼40초보다 높게 나타났음을 확인할 수 있었다. 또한, 실시예 1의 쌀맥주 알코올 함량이 8.34%로 나타나 알코올 함량 4.3∼6.17%인 비교예 1∼6의 맥주보다 높게 나타났다.As shown in Table 4, the bubble retention time of the rice beer produced by the two-stage fermentation with the rice grown in the first stage fermented with the rice roasted with the rice roasted in Example 1 was 45 seconds, The beer of rice beer and the beer of Comparative Example 6 was higher than the bubble retention time of 20 to 40 seconds. In addition, the rice beer alcohol content of Example 1 was 8.34%, which was higher than that of Comparative Examples 1 to 6 where the alcohol content was 4.3 to 6.17%.
아울러, 쌀맥주의 관능 평가를 수행한 결과, 표 5에 나타난 바와 같이, 실시예 1의 쌀맥주의 관능 평가 수치가 비교예 1∼6의 맥주보다 높게 나타났음을 확인할 수 있었다.As a result of sensory evaluation of the rice beer, it was confirmed that the sensory evaluation value of the rice beer of Example 1 was higher than that of the beer of Comparative Examples 1 to 6, as shown in Table 5.
결국, 본 발명의 1단 발효 및 2단 발효로 무증자된 쌀 및 증자된 쌀을 다양한 조합으로 이용하여 맥주를 제조할 경우, 기존방법(소요시간: 15일 이상)에 비해 제조시간(5∼8일)이 짧고, 상기 방법으로 제조된 쌀맥주는 검품 유지 시간, 알코올 함량, 당도 및 관능 평가 면에서 기존 맥주보다 전반적으로 우수함을 확인할 수 있었다. 특히 무증자된 쌀로 1단 발효시킨 다음, 증자된 쌀 55 중량%와 증자된 보리 45 중량%을 혼합하여 2단 발효시켜 제조된 쌀맥주의 관능이 가장 우수하게 나타났다.
As a result, when the beer is produced using various combinations of the rice grown in the first stage fermentation and the second stage fermentation in the present invention, the production time (5 ~ 8 days) was short, and it was confirmed that rice beer produced by the above method was generally superior to conventional beer in terms of inspection time, alcohol content, sugar content and sensory evaluation. In particular, the rice beer fermented by two stages of a mixture of 55 wt% of the increased rice and 45 wt% of the increased rice showed the highest sensory value.
실시예Example 4: 4: 무증자된Cashless 쌀, 증자된 쌀 및 증자된 보리 혼합물에 첨가된 맥아 종류에 따른 쌀맥주의 품질 특성 Quality Characteristics of Rice Brewed According to the Types of Malt Added to Rice, Roasted Rice and Roasted Barley Mixture
실시예 3에서 무증자된 쌀로 1단 발효시킨 다음, 증자된 쌀 55 중량%(알코올 8.4%)을 이용하여 2단 발효시켜 제조된 쌀맥주의 관능이 가장 우수하게 나타났으므로 이 조건에서 실험을 수행하였다. 증자된 쌀 55 중량%를 증자된 보리 45 중량%에 배합한 다음, 실시예 1의 방법으로 약 2일간 1단 발효시켰다. 이에 0.7%, 1.2% 또는 1.6%(w/w) 맥아(필스너(Pilsner), 페일(Pale) 또는 블랙 패턴트(Black patent))를 첨가하고, 약 3일간 2단 발효시킨 다음, 상기 발효물을 여과하여 쌀맥주로 수득하였다. 상기 쌀맥주에 대한 관능 평가는 35명의 일반 직장인으로 구성된 평가단을 통하여 외관(색감), 향미, 전체적인 맛, 바디감 및 종합적 평가에 대하여 9점 척도법으로 측정하였다.
The rice beer prepared by two stages fermentation using 55 wt% (alcohol: 8.4%) of the increased rice was most excellent in the sensory performance of the rice beer after one stage fermentation with the rice added in Example 3, Respectively. 55% by weight of the increased rice was blended in 45% by weight of the added barley and then fermented for 1 day by the method of Example 1 for about 2 days. Then, malt (Pilsner, Pale or Black patent) of 0.7%, 1.2% or 1.6% (w / w) was added and fermentation was performed in two stages for about 3 days. Was obtained as a rice beer by filtration. Sensory evaluation of the rice beer was carried out by a nine-point scale method for appearance (color), flavor, overall taste, body sensation and comprehensive evaluation through an evaluation team composed of 35 ordinary workers.
하기 표 6은 2단 발효에서의 증자된 쌀 55 중량% 및 증자된 보리 45 중량% 혼합물에 첨가된 맥아 종류에 따른 쌀맥주의 감각 평가(감미 효과)를 나타낸 것이다(도 4).Table 6 below shows the sensory evaluation (sweetening effect) of rice beer according to the type of malt added to a mixture of 55% by weight of the rice added in the two-stage fermentation and 45% by weight of the added rice barley (FIG. 4).
그 결과, 표 6 및 도 4에 나타난 바와 같이, 2단 발효에서 필스너(pilsner) 맥아가 첨가된 증자된 쌀 55 중량%(알코올 8.4%)로 제조된 쌀맥주의 관능이 가장 우수한 것으로 나타났다.
As a result, as shown in Table 6 and FIG. 4, the rice beer made from 55% by weight (alcohol 8.4%) of the enhanced rice added with pilsner malt in the 2-stage fermentation showed the best sensation.
실시예Example 5: 호프 종류 및 호프 첨가량에 따른 쌀맥주의 품질 특성 5: Quality characteristics of rice beer according to the amount of hops and hops
실시예 3에서 무증자된 쌀로 1단 발효시킨 다음, 증자된 쌀 55 중량%(알코올 8.4%)을 이용하여 2단 발효시켜 제조된 쌀맥주의 관능이 가장 우수하게 나타났으므로 이 조건에서 실험을 수행하였다. 쌀맥주에 첨가된 호프(hop)의 종류(사츠(sazz), 캐스캐이드(cascade), 캐스캐이드 및 고수(coriander)의 혼합물 또는 너겟(nugget)) 및 이의 첨가량(2,4, 3.2, 4.0, 4.8% w/v)에 따른 여과된 쌀맥주의 알코올 함량(%)은 비중계를 이용하여 1일 간격으로 측정하였고, 맥주의 관능 또한 평가하였다. 상기 쌀맥주에 대한 관능 평가는 35명의 일반 직장인으로 구성된 평가단을 통하여 외관(색감), 향미, 전체적인 맛, 바디감 및 종합적 평가에 대하여 9점 척도법으로 측정하였다.
The rice beer prepared by two stages fermentation using 55 wt% (alcohol: 8.4%) of the increased rice was most excellent in the sensory performance of the rice beer after one stage fermentation with the rice added in Example 3, Respectively. (Saz, cascade, cascade and coriander mixture or nugget) added to rice beer and its addition amount (2,4, 3.2, 4.0, 4.8% w / v) of alcoholic beverages were measured at 1-day intervals using a densitometer and the sensory evaluation of beer was also evaluated. Sensory evaluation of the rice beer was carried out by a nine-point scale method for appearance (color), flavor, overall taste, body sensation and comprehensive evaluation through an evaluation team composed of 35 ordinary workers.
하기 표 7은 2단 발효에서의 증자된 쌀 55 중량% 및 증자된 보리 45 중량% 혼합물에 첨가된 호프 종류에 따른 쌀맥주의 감각 평가(감미 효과)를 나타낸 것이다(도 5).Table 7 below shows the sensory evaluation (sweetening effect) of rice beer according to the kind of hops added to the mixture of 55% by weight of the rice added in the two-stage fermentation and 45% by weight of the added rice barley (FIG.
하기 표 8은 2단 발효에서의 증자된 쌀 55 중량% 및 증자된 보리 45 중량% 혼합물에 첨가된 호프의 첨가량에 따른 쌀맥주의 감각 평가(감미 효과)를 나타낸 것이다(도 6).Table 8 below shows the sensory evaluation (sweetening effect) of rice beer according to the addition amount of the hops added to the mixture of 55% by weight of the rice added in the two-stage fermentation and 45% by weight of the added barley (FIG. 6).
그 결과, 표 7, 표 8, 도 5 및 도 6에 나타난 바와 같이, 호프 중 캐스캐이드 2.4%(알코올 7.5%) 및 캐스캐이드 4.8%(알코올 7.1%)가 첨가된 쌀맥주에서 관능이 가장 우수한 것으로 나타났다.
As a result, as shown in Table 7, Table 8, Fig. 5 and Fig. 6, in the rice beer containing 2.4% cacid (7.5% alcohol) and 4.8% cacid (alcohol 7.1% The best.
실시예Example 6: 맥주 효모 종류에 따른 쌀맥주의 품질 특성 6: Quality characteristics of rice beer according to kinds of brewer yeast
실시예 3에서 무증자된 쌀로 1단 발효시킨 다음, 증자된 쌀 55 중량%(알코올 8.4%)을 이용하여 2단 발효시켜 제조된 쌀맥주의 관능이 가장 우수하게 나타났으므로 이 조건에서 실험을 수행하였다. 1단 발효 및 2단 발효에서 맥주 효모(Safale S-04, Safale US-05, Safbrew WB-06, Saflager S-23, Safbrew S-33, Saflager W-34/70 또는 Safbrew T-58)를 첨가한 다음, 약 5일간 발효시켰다. 그 다음, 각각의 서로 다른 맥주 효모로부터 수득한 발효물을 여과하여 쌀맥주를 수득하였다. 상기 쌀맥주의 알코올 함량(%)은 비중계를 이용하여 1일 간격으로 측정하였다. 또한, 상기 쌀맥주에 대한 관능 평가는 35명의 일반 직장인으로 구성된 평가단을 통하여 외관(색감), 향미, 전체적인 맛, 바디감 및 종합적 평가에 대하여 9점 척도법으로 측정하였다.
The rice beer prepared by two stages fermentation using 55 wt% (alcohol: 8.4%) of the increased rice was most excellent in the sensory performance of the rice beer after one stage fermentation with the rice added in Example 3, Respectively. The addition of beer yeast (Safale S-04, Safale US-05, Safbrew WB-06, Saflager S-23, Safbrew S-33, Saflager W-34/70 or Safbrew T-58) And fermented for about 5 days. Then, the fermented product obtained from each different brewer's yeast was filtered to obtain a rice beer. The alcohol content (%) of the rice beer was measured at intervals of one day using a specific gravity meter. The sensory evaluation of the rice beer was performed by a nine-point scale method for appearance (color), flavor, overall taste, body sensation, and comprehensive evaluation through an evaluation team composed of 35 ordinary workers.
하기 표 9는 2단 발효에서의 증자된 쌀 55 중량% 및 증자된 보리 45 중량% 혼합물에 첨가된 효모의 종류에 따른 쌀맥주의 감각 평가(감미 효과)를 나타낸 것이다(도 8).Table 9 below shows the sensory evaluation (sweetening effect) of rice beer according to the kind of yeast added to a mixture of 55% by weight of the rice added in the two-stage fermentation and 45% by weight of the added rice barley (FIG. 8).
그 결과, 도 7, 표 9 및 도 8에 나타난 바와 같이, 맥주 효모 중 Safale S-04(알코올 7.8%)가 첨가된 쌀맥주에서 관능이 가장 우수한 것으로 나타났다.
As a result, as shown in FIG. 7, Table 9, and FIG. 8, the rice beer containing Safale S-04 (alcohol 7.8%) among the beer yeast was found to have the best sensory properties.
실시예Example 7: 7: 흑맥아Black malt 첨가량에 따른 Depending on the amount added 쌀맥주의Rice beer 품질 특성 Quality characteristics
실시예 3에서 무증자된 쌀로 1단 발효시킨 다음, 증자된 쌀 55 중량%(알코올 8.4%)을 이용하여 2단 발효시켜 제조된 쌀맥주의 관능이 가장 우수하게 나타났으므로 이 조건에서 실험을 수행하였다. 약 2일간 1단 발효시켜 수득한 발효물에 0.7%, 1.2% 또는 1.6%(w/v) 흑맥아(Black malt)를 첨가하고, 약 3일간 2단 발효시킨 다음, 여과하여 쌀맥주를 수득하였다. 상기 쌀맥주의 알코올 함량(%)은 비중계를 이용하여 1일 간격으로 측정하였다. 또한, 첨가량에 따른 맥주의 관능을 평가하였다. 상기 쌀맥주에 대한 관능 평가는 35명의 일반 직장인으로 구성된 평가단을 통하여 외관(색감), 향미, 전체적인 맛, 바디감 및 종합적 평가에 대하여 9점 척도법으로 측정하였다.
The rice beer prepared by two stages fermentation using 55 wt% (alcohol: 8.4%) of the increased rice was most excellent in the sensory performance of the rice beer after one stage fermentation with the rice added in Example 3, Respectively. Black malt (0.7%, 1.2% or 1.6% (w / v)) was added to the fermented product obtained by fermentation for about 2 days, and the fermented product was fermented in two stages for about 3 days and then filtered to obtain rice beer Respectively. The alcohol content (%) of the rice beer was measured at intervals of one day using a specific gravity meter. In addition, the sensory evaluation of the beer according to the addition amount was evaluated. Sensory evaluation of the rice beer was carried out by a nine-point scale method for appearance (color), flavor, overall taste, body sensation and comprehensive evaluation through an evaluation team composed of 35 ordinary workers.
하기 표 10은 2단 발효에서의 증자된 쌀 55 중량% 및 증자된 보리 45 중량% 혼합물에 첨가된 흑맥아의 첨가량에 따른 쌀맥주의 감각 평가(감미 효과)를 나타낸 것이다(도 10).Table 10 below shows the sensory evaluation (sweetening effect) of rice beer according to the addition amount of black malt added to a mixture of 55% by weight of the rice added in the two-stage fermentation and 45% by weight of the added barley (FIG. 10).
그 결과, 도 9, 표 10 및 도 10에 나타난 바와 같이, 1.2% 흑맥아가 첨가된 쌀맥주에서의 알코올 함량이 8.5%로 가장 높았고, 관능이 가장 우수한 것으로 나타났다.
As a result, as shown in FIG. 9, Table 10 and FIG. 10, the alcohol content in the rice beer with 1.2% black malt added was the highest at 8.5%, and the sensory performance was the highest.
실시예Example 8: 8: 쌀맥주와Rice beer and 자색고구마 맥주의 품질 특성 비교 Comparison of quality characteristics of purple sweet potato beer
실시예 3에서 무증자된 쌀로 1단 발효시킨 다음, 증자된 쌀 55 중량%(알코올 8.4%)을 이용하여 2단 발효시켜 제조된 쌀맥주의 관능이 가장 우수하게 나타났으므로, 이 조건에서 쌀맥주와 자색고구마 쌀맥주를 제조한 다음, 감각 평가를 수행하였다. The rice beer produced by two stages fermentation using 55 wt% (alcohol: 8.4%) of the increased rice was the most excellent in the sensory performance of the rice beer after one stage fermentation with the unsupplemented rice in Example 3, Beer and purple sweet potato rice beer were prepared and sensory evaluation was performed.
실시예Example 1: One: 쌀맥주Rice beer
실시예 1의 쌀맥주 제조방법과 동일하게 증자된 쌀 55 중량% 및 증자된 보리 45 중량%를 1단 발효물에 첨가하여 2단 발효시켜 제조하였다.
In the same manner as in the rice beer manufacturing method of Example 1, 55% by weight of the rice added and 45% by weight of the increased barley were added to the first stage fermentation product to prepare two stages of fermentation.
비교예Comparative Example 1: 자색고구마가 추가된 1: Added purple sweet potato 쌀맥주Rice beer
실시예 1의 쌀맥주 제조방법과 동일하며, 전체량의 8%(w/v)가 되도록 자색고구마 분쇄물을 1단 발효물에 포함시켜 2단 발효시켜 제조하였다.
The same procedure as in the rice beer manufacturing method of Example 1 was carried out by adding the purple sweet potato pulverized product to the first stage fermentation product so as to have 8% (w / v) of the total amount.
상기와 같이 제조된 실시예 1과 비교예 1의 관능 평가를 수행하였다. 상기 맥주(들)에 대한 관능 평가는 35명의 일반 직장인으로 구성된 평가단을 통하여 외관(색감), 냄새, 향미, 목넘김 및 종합기호도에 대하여 9점 척도법으로 측정하였다.
The sensory evaluation of the thus prepared Example 1 and Comparative Example 1 was carried out. The sensory evaluation of the beer (s) was carried out by a 9-point scaling method on appearance (color), smell, flavor, flavor, and overall preference through an evaluation team consisting of 35 ordinary employees.
하기 표 11은 무증자된 쌀, 증자된 쌀 및 증자된 보리 혼합물을 이용하여 2단 발효로 제조된 쌀맥주와 자색고구마 쌀맥주를 비교하여 감각 평가(감미 효과)한 것이다.Table 11 below shows the sensory evaluation (sweetness effect) of rice beer and purple sweet potato rice beer prepared by two stages of fermentation using freeze-dried rice, roasted rice and roasted barley mixture.
그 결과, 표 11에 나타난 바와 같이, 증자된 쌀 55 중량% 및 증자된 보리 45 중량%가 첨가된 실시예 1의 쌀맥주 및 비교예 1의 자색고구마 쌀맥주는 대등한 관능을 나타내는 것으로 확인되었다.
As a result, as shown in Table 11, it was confirmed that the rice beer of Example 1 and the purplish sweet potato rice beer of Comparative Example 1, to which 55% by weight of the increased rice and 45% by weight of the increased barley were added, showed comparable sensory properties .
이상으로 본 발명 내용의 특정한 부분을 상세히 기술하였는바, 당업계의 통상의 지식을 가진 자에게 있어서, 이러한 구체적 기술은 단지 바람직한 실시양태일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백할 것이다. 따라서 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의하여 정의된다고 할 것이다. While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereby. something to do. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.
Claims (8)
(a) 쌀을 1∼3시간 동안 물에 침지하고, 분쇄시킨 무증자된 쌀에 액화효소제, 당화효소제, 효모 및 물을 첨가한 다음, 15∼25℃에서 1∼3일간 발효시키는 1단 발효 단계;
(b) 상기 (a) 단계에서 준비된 1단 발효물에 증자된 쌀과 증자된 보리 및 증자된 맥아를 추가로 혼합시키고, 상기 혼합되는 쌀과 보리의 함량비는 45∼55 중량%:55∼45 중량%이고, 팽화미, 보리, 맥아, 액화효소제, 당화효소제, 효모 및 물을 첨가한 다음, 15∼25℃에서 0.5∼1.5일간 발효시킨 후 호프를 첨가하고, 15∼25℃에서 1∼3일간 발효시키는 2단 발효 단계; 및
(c) 상기 (b) 단계에서 수득한 2단 발효물을 여과하여 쌀맥주로 수득하는 단계.
A method for producing rice beer using a mixture of roasted rice, roasted rice and roasted barley comprising the steps of:
(a) a step of immersing the rice in water for 1 to 3 hours, adding a liquefying enzyme, a saccharifying enzyme, yeast and water to the pulverized rice without sugar, and then fermenting for 1 to 3 days at 15 to 25 ° C step;
(b) adding the rice added to the one-stage fermented product prepared in the step (a), the boiled barley and the boiled malt, and mixing the rice and the barley in an amount of 45 to 55 wt% 45 to 45% by weight, and after adding boiled rice, barley, malt, liquefying enzyme, saccharifying enzyme, yeast and water, fermenting at 15 to 25 ° C for 0.5 to 1.5 days, adding a hop, A two-stage fermentation step of fermenting for three days; And
(c) filtering the two-stage fermentation product obtained in step (b) to obtain a rice beer.
The method of manufacturing rice beer according to claim 1, wherein the rice added is rice bran steamed at 95 to 100 DEG C for 15 to 45 minutes after rice is immersed in water for 1 to 3 hours, .
The method according to claim 1, wherein the boiled barley and the boiled malt are steamed barley and malt steamed at 95 to 100 ° C for 15 to 45 minutes after immersing the barley and malt in water for 1.5 to 4.5 hours, ≪ / RTI >
The method of claim 1, wherein the liquefying enzyme is selected from the group consisting of Bacillus sp . ) -Amylase derived from rice bran.
The method according to claim 1, wherein the saccharifying enzyme is Aspergillus or Aspergillus amylase derived from a niger- derived β-amylase.
[4] The method of claim 1, wherein the content of rice and barley further mixed in the first stage fermented product in step (b) is 45 to 55 wt%: 55 to 45 wt%.
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