KR101562604B1 - Manufacturing method of wound gauze and the wound gauze manufactured by the same - Google Patents

Abstract

The present invention relates to a method for manufacturing a wound gauze which functions in healing wound and preventing fusion, through complexation by treating a gauze with chitosan which is a cationic polysaccharide and also with polysaccharides containing anionic groups including a carboxyl group, a sulfate group, and a phosphate group. In addition, the present invention relates to the wound gauze.

Description

TECHNICAL FIELD [0001] The present invention relates to a gouging method and a gouging method,

The present invention relates to a method for manufacturing a dough gauze having a wound healing function and a fusion prevention function by treating a gauze with a polysaccharide having an anionic carboxyl group, a sulfate group and a phosphoric acid group and a chitosan as a cationic polysaccharide.

More specifically, the anion polysaccharide and chitosan are mixed to form a composite, and then dressing is applied to the wound to form an ion complex between the materials to gelify the material. When the gauze is changed by maintaining wettability, the wound and gauze are not fused It's about the gowns.

Skin damage due to various causes such as severe burns, trauma, wounds, pressure sores and skin diseases is common. For example, wounds that can not be primaryly implanted may leave severe defects inevitably resulting from skin grafting. Therefore, restoration of damaged skin tissue is a very important issue. Wound treatment using proper dressing is indispensable to accelerate the treatment of wounds and minimize secondary side effects.

Acute wounds in most widespread areas are infected, and bacterial contamination can occur in the case of chronic wounds, and in virtually all cases, some cellular necrosis is present. These broad subacute and chronic wounds are very difficult to treat. Presently, it is desirable to have a system that maintains a permanent moist environment for healing large areas of wounds or chronic wounds. This system comes from a theory formulated in the 1960s that proves that wet wounds by winter are cured two times faster than dry wounds.

In the case of an open wound exposed to air, a scab is formed which acts as a physical boundary for dehydration and epithelial cell migration. Then, the healing process should proceed to deep tissue, which significantly delays the healing process.

The easiest way to create a moist environment is to repeatedly bandage the moist gauze. The gauze is moistened with a physiological solution or disinfectant-containing solution. In this method, the bandages should be changed frequently every 4 to 6 hours.

However, this method does not completely disinfect the wound, and also does not prevent skin rash on the wound periphery.

In addition, there is a problem that if the bandages are not kept damp regularly, the gauze becomes dry and sticks to the wound, so it is very painful to remove the gauze to wait for the bandage again.

Korean Patent Publication No. 10-2010-0077186 (published on July 7, 2010)

Winter G.D .: Formation of scab and rate of epithelialization of superficial wounds in the skin of the young domestic pig; Nature 1962, 193, 293 Ovington L. G .: Advances in wound dressings; Clinics Dermatol 2007, 25, 33 Queen D., Evans J. H., Gaylor J. D. S. et al .: Burn wound dressings - a review; Burns1987, 13, 218 Falabella A.F .: Debridement and wound bed preparation; Dermatol Therapy 2006, 19, 317

In order to solve the above problems, the present invention provides a method for producing a gut having a function of treatment for wound healing and prevention of fusion by treating a gauze with a polysaccharide having an anionic carboxyl group, a sulfate group and a phosphate group and a cationic polysaccharide chitosan, It is an object of the present invention to provide a unid gauze prepared from the above.

In order to achieve the above object,

The present invention relates to a process for preparing a mixed solvent by mixing water and an organic solvent at a volume ratio of 1: 9 to 7: 3,

Mixing the anionic polysaccharide powder and the water-soluble chitosan in a weight ratio of 9: 1 to 1: 9 to prepare a mixed polysaccharide powder;

0.1 to 50 wt% of the mixed polysaccharide powder is added to 50 to 99.9 wt% of a mixed solvent to uniformly mix and disperse to form a mixed polysaccharide dispersion solution,

Spraying or impregnating the mixed polysaccharide dispersion solution into the gauze,

Spraying or impregnating the gauze to form a multidose ion complex while dissolving the mixed polysaccharide in the process of drying, thereby adhering the mixed polysaccharide to the gauze and attaching the gauze;

To provide the guddies produced by the method of making guddies.

The unid gauze prepared according to the present invention absorbs the exudate from the wound area, maintains the suction of the wound area to quickly heal the wound, and prevents the gauze from sticking to the wound, Can be produced.

Hereinafter, the technical composition of the present invention will be described in more detail.

As described above,

The < RTI ID = 0.0 >

Mixing water and an organic solvent in a volume ratio of 1: 9 to 7: 3 to prepare a mixed solvent;

Mixing the anionic polysaccharide powder and the water-soluble chitosan in a weight ratio of 9: 1 to 1: 9 to prepare a mixed polysaccharide powder;

0.1 to 50 wt% of the mixed polysaccharide powder is added to 50 to 99.9 wt% of a mixed solvent to uniformly mix and disperse to form a mixed polysaccharide dispersion solution,

Impregnating the mixed polysaccharide dispersion solution with gauze,

Sprayed or impregnated gauze is dried to form a multidose ion complex while dissolving the mixed polysaccharide in the drying process, and the mixed polysaccharide is adhered to the gauze and adhered to it.

Examples of the organic solvent include alcohols such as methyl alcohol, ethyl alcohol, (isopropyl) alcohol, (t-isobutyl) alcohol, ethylene glycol and diethylene glycol;

Acetone, ethyl methyl ketone, diethyl ketone, methyl propyl ketone, ketones of ethyl propyl ketone; Or one or more selected from among them.

At this time, in selecting and using an organic solvent, it is preferable to use ethanol and methanol as alcohols and acetone as ketones.

As described above, the mixed solvent is used by mixing with water and an organic solvent, and is used within a range in which the anionic polysaccharide fine powder and the water-soluble chitosan are not dissolved.

The mixing ratio of water and organic solvent constituting the mixed solvent is maintained in a volume ratio of 1: 9 to 7: 3.

When the volume ratio of water is less than 1, there is a problem that the gel is not formed. When the ratio exceeds 7, the polysaccharide is dissolved and the viscosity is too high to impregnate.

When the volume ratio of the organic solvent is less than 3, the polysaccharide is dissolved and the viscosity is too high to be impregnated. There is a problem that a complex is formed and precipitated before spraying or impregnation. When the organic solvent exceeds 9, gel is not formed there is a problem.

For this reason, the mixed solvent is preferably prepared by mixing water and an organic solvent at a volume ratio of 1: 9 to 7: 3.

The anionic polysaccharide may be a polysaccharide having a carboxyl group of carboxymethyl cellulose (CMC), carboxyethyl cellulose, carboxymethyl beta glucan (CM-β-Glucan), carboxymethyl chitosan, succinyl chitosan, alginic acid or hyaluronic acid;

Sulfolinyl cellulose, sulfonylbeta glucan, sulfonyl chitosan, heparin, polysulfide having a sulfone group of chondroitin sulfate;

A polysaccharide having a phosphate group of phosphorylated cellulose, phosphorylated chitosan, and beta-glucan phosphate; Or one or more selected from among them.

The anionic polysaccharide used is a fine powder of 200 mesh or more, more specifically, a fine powder of 200 to 1,000 mesh is used. The molecular weight of the polysaccharide is from 1,000 g / mole to 3,000,000 g / mole.

The water-soluble chitosan is one selected from chitosan hydrochloride, chitosan acetate or chitosan chitosan having a degree of deacetylation of 50% or more and a molecular weight of 1,000 g / mole to 3,000,000 g / mole.

Specific examples of the water-soluble chitosan include beta -methylcellulose prepared by treating shrimp shell, crab shell and the like with caustic soda and treated with hydrochloric acid to obtain deacetylated chitin, - Water-soluble chitosan prepared by treating chitosan with hydrochloric acid, acetic acid and lactic acid is used.

At this time, chitosan uses fine powder of 200 mesh or more, more specifically, fine powder of 200 to 1,000 mesh is used. It is also preferable to use chitosan having a molecular weight of 1,000 g / mole to 3,000,000 g / mole, more specifically chitosan having a molecular weight of 10,000 g / mole to 1,000,000 g / mole.

As described above, the mixed polysaccharide powder is prepared by mixing an anionic polysaccharide powder and a water-soluble chitosan in a weight ratio of 9.9: 0.1 to 0.1: 9.9,

At this time, when the weight ratio of the anionic polysaccharide fine powder is less than 0.1, there is a problem that a complex due to gelation can not be formed, and even when it exceeds 9.9, a complex can not be formed.

When the weight ratio of the water-soluble chitosan is less than 0.1, there is a problem that a complex due to gelation can not be formed, and even when the weight ratio exceeds 9.9, a complex can not be formed.

For this reason, the mixed polysaccharide powder is preferably prepared by mixing an anionic polysaccharide powder and a water-soluble chitosan in a weight ratio of 9: 1 to 1: 9.

The mixed polysaccharide powder is added to the mixed solvent at a blending ratio of 50 to 99.9 wt% of the mixed solvent and 0.1 to 50 wt% of the mixed polysaccharide powder.

When the amount of the mixed solvent used is less than 50 wt%, the viscosity is too high to cause deposition problems. When the amount exceeds 99.9 wt%, the amount of the mixed solvent is too small. Is preferably within a range of 50 to 99.9 wt%.

If the amount of the mixed polysaccharide powder used is less than 0.1 wt% or more than 50 wt%, the same amount of the mixed solvent is used. Therefore, the amount of the mixed solvent used is preferably in the range of 0.1 to 50 wt% It is preferable to limit it.

The mixed polysaccharide powder is added to the mixed solvent to form a mixed polysaccharide dispersion solution, and the mixed polysaccharide dispersion solution thus prepared is impregnated with gauze.

In this case, the gauze is made of any one of a nonwoven fabric, a cotton, and a fabric made of a material selected from cotton, wool, rayon, polyester fiber, polyacrylic fiber or a mixture thereof.

The gauze has a polygonal shape such as a quadrangle, or has a circular shape,

The surface is embossed or has holes for breathability,

The rim is used by keeping the cutting surface state as it is or sealing it as a whole.

And the impregnation is accomplished by a method selected from spraying, deposition, or application.

The gauze impregnated with the mixed polysaccharide dispersion solution is subjected to a drying process in which the drying is carried out at a temperature ranging from room temperature to 200 ° C.

If the temperature falls below room temperature, there is a problem that the drying time is too long. When the temperature exceeds 200 ° C, there is a problem of discoloration. Therefore, the drying temperature is preferably within a range of room temperature to 200 ° C.

During the drying process, the mixed polysaccharide is dissolved to form a multibranched ion complex, and the mixed polysaccharide is gelled and adhered to the gauze.

At this time, a binder may be further used to improve the adhesiveness of the polysaccharide to the gauze. At least one selected from among ethyl cellulose, acrylic binder and urethane binder is used as the binder.

The amount of the binder to be used is determined within a range that does not deteriorate the absorbency of the gauze. Specifically, it is used within a range of 0.5 to 5 wt% based on the total weight of the mixed polysaccharide dispersion solution.

The unid gauze manufactured through this process is crosslinked by the ionic complex between the polysaccharide and the anion polysaccharide and the water-soluble chitosan to form a gel, thereby improving adhesion to the gauze. When the wound is treated, the proper wetting is maintained, In addition to being able to replace the wool gauze without pain can be replaced.

Further, since the present invention is produced by dispersing polysaccharide in an organic mixed solvent without dissolving the polysaccharide, it does not tangle with each other because it is sprayed, deposited, or applied to gauze (nonwoven fabric, fabric, cotton) while preventing the ion complex in the manufacturing process. In addition, since the organic solvent is dried first and only the water is left in the drying process, the mixed polysaccharide is partially dissolved to form mutual ion complexes and adhere to the gauze, so that the adhesive force is excellent. Provides a new Unwound Gauze.

Hereinafter, the technical contents of the present invention will be described in detail.

A 1,000 mL round flask was mixed with water and ethanol at a volume ratio of 50:50, and 475 mL of the mixed solvent was added thereto.

Next, 40 g of sodium alginate (molecular weight: 220,000 g / mol) and 10 g of water-soluble chitosan (25,000 g / mol) were mixed to prepare a mixed powder, which was then added to the mixed solvent and dispersed with stirring.

When the dispersion of the mixed powder is completed, the completed dispersion is put in a sprayer, sprayed on a nonwoven fabric, and then dried at 170 ° C for 5 minutes to prepare a pollen-treated unid gauze.

The same procedure as in Example 1 was followed to produce a dough gauze,

However, in the case of using a mixed solvent, water and ethanol are mixed at a volume ratio of 70:30 to prepare a mixed gauze.

The same procedure as in Example 1 was followed to produce a dough gauze,

However, in using a mixed solvent, 4 g of ethyl cellulose is added to ethanol to prepare a gut using a mixed solvent.

The same procedure as in Example 1 was followed to produce a dough gauze,

In the use of anionic polysaccharides, wool gauzes are prepared using carboxymethylcellulose (CMC) (250,000 g / mol) instead of sodium alginate.

A round gauze was obtained by treating the same in the same manner as in Example 1 except that acetone was used instead of ethanol in a 1,000 mL round flask, and a mixed solvent in which the volume ratio was 70:30 was used.

The same procedure as in Example 1 was followed to produce a dough gauze,

However, in the use of anionic polysaccharide, the gut is prepared by replacing sodium alginate with carboxymethylbetaglucan (CM-β-Glucan) (150,000 g / mol).

The dun gauzes prepared by the methods described in Examples 1 to 6 have a polygonal shape including a quadrangular shape or have a circular shape. The dun gauze thus prepared is used as it is, It is also possible to use embossing or embossing of the uned gauze presented in Example 6, or to maintain the cut edge condition of the ungut gauze as it is, or to use the whole of the rim as a sealing treatment.

The ungut gauze prepared in Examples 1 to 6 was perforated so that the ratio of the holes was 40% of the total area.

The unid gauze prepared through Examples 1 to 6 was subjected to embossing treatment in a dry manner to prepare a dough gauze.

The entire slope, which is the cut surface of the unid gauze prepared through Examples 1 to 6, is sealed to produce the unid gauze.

Hereinafter, experimental results of wound healing and adhesion separation of the unid gauze prepared in Examples 1, 4 and 6 will be presented.

[Test Example 1]

[ Wound healing  Experiment ]

The animals were tested with a model to see the wound healing effects of the gowns produced in Examples 1, 4 and 6. On the left and right sides of the abdominal wall of the rabbit, 12 x 10 mm wounds were made in the form of arrows, respectively. The suture was closed so that the suture was not exposed to the abdominal cavity while one side was covered with unid gauze and the other side was left as it was.

This result was observed after 2 weeks and 4 weeks later, the film dressing agent remained unchanged on the side where the unid gauze was used, and the healing process of the wound was observed, compared with the case where the scar was slightly left but not the unid gauze The chest appeared to be weak.

[Test Example 2]

[Adhesion Separation Experiment]

Adhesion separation and pain tests were performed by adhering to the wounds of the unid gauze prepared in Examples 1, 4, and 6. Twenty patients with wounds were examined for adhesion strength and pain at 3 days after fixation of 6 × 10 mm of unid gauze on wound.

0: Disconnected with light force and no pain

1: The adhesion is separated by a slight force and there is no pain

2: Adhesion is separated by moderate force and has slight pain

3: The adhesion is separated by a strong force, and there are some injuries and pain.

4: Very difficult adhesion due to very strong adhesion.

Adhesion strength and pain 0 One 2 3 4 Example 1 19 One - - - Example 4 15 5 - - - Example 6 18 2 - - - Untreated gauze - - - 2 18

As a result of the investigation of adhesion strength and pain as described above, the gauze treated with the polysaccharide mixture did not feel the adhesion strength and pain, but when it was untreated, the pain was severe and the adhesion strength was very strong. Therefore, it was confirmed that the unid gauze prepared according to the present invention can prevent the adhesion more effectively and effectively treat wounds.

The unid gauze according to the present invention has a high possibility of being used in the medical industry because it is suitable for use for wound care and treatment without pain and high healing effect by preventing adhesion and providing absorption ability.

Claims (7)

Mixing water and an organic solvent in a volume ratio of 1: 9 to 7: 3 to prepare a mixed solvent;
Mixing the anionic polysaccharide powder and the water-soluble chitosan in a weight ratio of 9: 1 to 1: 9 to prepare a mixed polysaccharide powder;
0.1 to 50 wt% of the mixed polysaccharide powder is added to 50 to 99.9 wt% of a mixed solvent to uniformly mix and disperse to form a mixed polysaccharide dispersion solution,
Impregnating the mixed polysaccharide dispersion solution with gauze,
Drying the impregnated gauze to form a multidose ion complex with dissolution of the mixed polysaccharide during the drying process to adhere the gauze to the mixed polysaccharide and adhere to the gauze.
The method according to claim 1,
Examples of the organic solvent include alcohols such as methyl alcohol, ethyl alcohol, (iso) propyl alcohol, (t-iso) butyl alcohol, ethylene glycol and diethylene glycol;
Acetone, ethyl methyl ketone, diethyl ketone, methyl propyl ketone, ketones of ethyl propyl ketone; Wherein the at least one kind of the gauze is at least one selected from the group consisting of at least two kinds of gauze.
The method according to claim 1,
Anionic polysaccharides include polysaccharides having carboxy groups of carboxymethyl cellulose (CMC), carboxyethyl cellulose, carboxymethyl betaglucan (CM-beta-Glucan), carboxymethyl chitosan, succinyl chitosan, alginic acid, and hyaluronic acid;
Sulfolinyl cellulose, sulfonylbeta glucan, sulfonyl chitosan, heparin, polysulfide having a sulfone group of chondroitin sulfate;
A polysaccharide having a phosphate group of phosphorylated cellulose, phosphorylated chitosan, and beta-glucan phosphate; Or a combination of two or more species selected from among them,
And the molecular weight of the polysaccharide is 1,000 g / mole to 3,000,000 g / mole.
The method according to claim 1,
Soluble chitosan is one or more selected from chitosan hydrochloride, chitosan acetate or chitosan chitosan having a deacetylation degree of at least 50%, a fine powder of 200 to 1,000 meshes and a molecular weight of 1,000 g / mole to 3,000,000 g / mole ≪ / RTI >
The method according to claim 1,
Wherein the gauze is one selected from the group consisting of cotton, wool, rayon, polyester fiber and polyacrylic fiber.
The method according to claim 1,
Wherein the binder is ethyl cellulose, an acrylic binder, or a urethane binder in order to improve adhesion to the gauze of the polysaccharide.
Wherein the gauze is made by the manufacturing method of claim 1.