KR101536224B1 - A composition comprising the fruit extract of Platycarya strobilacea having skin whiting activity - Google Patents

Abstract

The present invention relates to a composition for whitening skin, which contains an extract of Aspergillus oryzae as an active ingredient. More specifically, the present invention relates to a skin whitening pharmaceutical composition and a cosmetic composition containing a crude extract, a polar solvent extract or a nonpolar solvent extract as an active ingredient . The extract of the present invention effectively inhibits tyrosinase activity, which is an enzyme essential for the melanin biosynthetic process, and inhibits biosynthesis of melanin in B16 melanoma cells, so that the extract can be effectively used as a whitening pharmaceutical composition and a cosmetic composition.

       Sorghum, tyrosinase, melanin biosynthesis, whitening

Description

FIELD OF THE INVENTION The present invention relates to a skin whitening composition containing an extract of Aspergillus oryzae,

The present invention relates to a composition for whitening skin, which contains an extract of Aspergillus oryzae as an active ingredient. More specifically, the present invention relates to a skin whitening pharmaceutical composition and a cosmetic composition containing a crude extract, a polar solvent extract or a nonpolar solvent extract as an active ingredient .

Melasma is a major factor in skin pigmentation, which is caused by an increase in melanin in the epidermis. Melanin is produced in the organelle called melanosomes in melanocytes that are present in the epidermal basal layer. That is, mitosis of melanocytes occurs by ultraviolet rays, and melanocytes are then activated. In the activated melanocyte, the synthesis of tyrosinase is promoted and the production of melanin is enhanced and transported and discharged out of the epidermis (VJ Hearing and M. Jimenez., Int. J. Biochem , 19 (20), p.1141, 1987 , I. Koiso., Fragrance J. 6 , p39, 1990).

Naturally occurring melanin is a mixture of alkaline insoluble black melanin (New melanin) and alkali soluble yellow or red melanin (Y. Tomita., Fragrance J , 6 , p20, 1990.) Polymers are often referred to as neomelanin, and Prota (G. Prota., J. Invest . Dermatol ., 75 , p122, 1980.) and Pavel (S. Pavel et al., Cancer Detection and Prevention , 6 , p311., 1983.) have shown that, in addition to the 5,6-dihydroxyindole that has been contemplated so far, neuraminin is a 5,6-dihydroxyindole- It is known that it is produced from bosan and various melanin intermediate metabolites. The melanin production process in melanomas is characterized in that tyrosine is oxidized by enzyme tyrosinase to result in dopa → dopaquinone → dopachrome → 5,6-dihydroxyindole → indole-5,6-quinone, followed by indole-5,6 It is known to produce melanin by polymerization of quinones. Among them, enzyme tyrosinase is involved in tyrosine-dopaquinone, and it is known that the autoxidation contributes thereafter. It is well known that the pigmentation caused by sunbathing, that is, the blackening phenomenon is a result of the increase of melanin production by the ultraviolet rays in sunlight.

The mechanism of inhibition of melanogenesis from the melanin production process in melanocytes, which has been clarified so far, is as follows. The mechanism of inhibiting melanogenesis by inhibiting the cytotoxicity of melanocytes and the inhibition of melanogenesis in melanocytes, And a mechanism of inhibitory action. Recently, it has been newly discovered that melanin is produced not only by tyrosinase but also by tyrosinase related protein 1 (TRP-1) and tyrosinase-related protein 2 (TRP-2, Dopachrome tautomerase) These are also important enzymes involved in the biosynthesis of melanin, and development of substances capable of inhibiting these enzymes can also be a way to prevent skin pigmentation (Marmol V. at al., FEBS Letter , 327 , p. 307, 1993; Winder AJ at al., Pigment Cell Research , 7 , p. 305, 1994).

As the tyrosinase activity inhibitors developed so far, there are known chelating agents for copper ions in the active site of tyrosinase, reducing agents such as vitamin C or kojic acid for reducing quinones to phenols, and bisulfite formulations for modifying tyrosinase itself . In addition, hydroquinone, retinol, hydrocortisol and the like are known as substances exhibiting a whitening effect by inhibiting tyrosinase activity.

Platycarya strobilacea is distributed in Korea (south of Chungcheongnam-do), Japan, Taiwan, and China. It grows in a sunny place at the foot of the mountain or in the watery rocky region of the beach. It is 5-20m high, 10-50cm high. Leaves are odd numbered haplotypes and are composed of 7-19 small leaves with no petiole, and the small leaves are oval-shaped or egg-shaped, with pointed ends and deep-valley sawtooth. Both sides of the leaf and peduncle have white hairs but gradually disappear. In May-June, a yellowish-green flower blooms, 5-8cm in the diameter of the male flower, and 2-4cm in the female flower. The adult female flower ear is in the shape of a pine cone, the fruit ears are long oval, blackish brown and have no hair. It does not fall down and it is tantalizing. The fruit is nuts with wings and ripen in September-October. Its main ingredients and efficacy include yuluron in its shell, it has strong insecticidal and bactericidal action, and is used for the treatment of acne vulgaris or head swelling of skin. The fruit is called tannin, and the fruit is called fungus and its effect is such as soreness, epidemic, giant wind, and it is effective for treating muscle pain, abdominal pain, toothache and eczema swelling. . The bark is used as a hair dye and the leaves are toxic, so it is prohibited to take.

        The present inventors searched for a substance effective for skin whitening and improvement, and found that the ingredients extracted from the extract of the sorghum fruit showed excellent skin whitening efficacy as a result of screening substances effective for skin whitening among various natural substances of nature And the present invention was completed.

       It is an object of the present invention to provide a skin whitening composition superior in stability and efficacy to the existing whitening compositions.

       It is an object of the present invention to provide a cosmetic composition or pharmaceutical composition containing as an active ingredient an extract of Zucchini fruit extract, a polar solvent extract or a non-polar solvent extract having an inhibitory effect on tyrosinase and a melanin biosynthesis of melanoma cells .

The present invention relates to Platycarya strobilacea ) fruit extract as an active ingredient, and a cosmetic composition for skin whitening.

The composition for skin whitening comprises an extract of Aspergillus oryzae, a polar solvent-soluble extract or a non-polar solvent-soluble extract as an active ingredient.

The extract can be extracted at room temperature or warmed under conditions where the active ingredient of the extract is not destroyed or the breakdown is minimized. Depending on the solvent to be extracted, the degree of extraction and the degree of loss of the active ingredient of the extract may differ. Therefore, a suitable solvent should be selected and used.

The extraction method is not particularly limited, and for example, cold extraction, ultrasonic extraction, reflux cooling extraction, room temperature extraction, hot water extraction and the like can be used depending on the kind of the extract and the extraction method.

Filtration is a process for removing suspended solid particles from an extract, and may be performed by filtering particles using a cotton, nylon, or the like, or by ultrafiltration, freezing filtration, centrifugation, etc., but is not limited thereto. In addition, it may further comprise a separation process by various chromatographies (size, charge, hydrophobicity or affinity chromatographic).

The step of drying the filtrate includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying and the like. In some cases, a step of pulverizing the final dried extract may be added.

In the composition of the present invention, the extract of Aspergillus oryzae is a crude extract extracted with water, a lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin or a mixed solvent thereof.

Alternatively, the extract may be prepared by dissolving the solid fraction of the crude extract in a non-polar solvent selected from a nucleic acid, chloroform, methylene chloride, ethyl acetate or a mixed solvent thereof or a polar solvent extracted from water, ethanol, butanol or a mixed solvent thereof .

The term " crude extract " as used herein includes extracts extracted from one or more solvents selected from the group consisting of water containing purified water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, butylene glycol and glycerin.

As used herein, the term " polar solvent-soluble extract " includes extracts extracted with one or more polar solvents selected from the group consisting of water, methanol, butanol,

As used herein, the term " nonpolar solvent-soluble extract " includes extracts that are extracted with one or more non-polar solvents selected from the group consisting of hexane, chloroform, methylene chloride or ethyl acetate.

Hereinafter, the crude extract, the polar solvent-soluble extract and the non-polar solvent-soluble extract of the present invention will be described in detail.

      The crude extract can be extracted with one or more solvents selected from the group consisting of water containing purified water, lower alcohols having 1 to 4 carbon atoms, butylene glycol, propylene glycol and glycerin as the extraction solvent.

The polar solvent-soluble extract may be extracted from the solid content of the crude extract by using one or more solvents selected from the group consisting of water, methanol, butanol or a mixed solvent thereof. Preferably, butanol can be used for extraction.

The non-polar solvent-soluble extract can be extracted from the solid component of the above crude extract using a non-polar solvent including hexane, chloroform, methylene chloride or ethyl acetate.

In one specific embodiment, the crude extract may be obtained by the following steps. Water containing purified water of 1 to 30 times, preferably 10 to 20 times its volume in dried peach fruit, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol and glycerin More than two branches; An extraction temperature of 30 to 100 DEG C, preferably 50 to 95 DEG C in a state where a cooling condenser is installed to prevent the solvent from evaporating; 1 to 30 hours, preferably 3 to 20 hours; 1 to 7 times, preferably 1 to 3 times; Extraction using cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, heat extraction, preferably hot water extraction; The obtained extract is filtered, concentrated under reduced pressure, and freeze-dried or spray-dried to obtain a dried extract.

Further, in one specific embodiment, the polar solvent and non-polar solvent-soluble extract of the present invention may be added to extracts of extracts of the sorghum fruit obtained through the above process, preferably ethanol extracts of oyster fruit, Preferably 7 to 6 times, preferably 4 to 6 times, water, and then fractionating the solution one to five times, preferably two to four times, by adding hexane, chloroform, methylene chloride, ethyl acetate and butanol in an equal amount to water .

The extract of Fagus cucumber extract obtained by the above production method is preferably in a weight ratio of 0.1 to 50%, more preferably 0.1 to 40%, most preferably 0.1 to 30% by weight, based on the total weight, .

The crude extract, the polar solvent-soluble extract, and the non-polar solvent-soluble extract show excellence in enhancing skin whitening through inhibition of tyrosinase activity and inhibition of melanin production.

In addition, the crude extract, the polar solvent-soluble extract, and the non-polar solvent-soluble extract have little toxicity and side effects, so that they can be safely used in the pharmaceutical composition and cosmetic composition of the present invention.

In one aspect, the present invention provides a pharmaceutical composition for skin whitening comprising an extract of Aspergillus oryzae, a polar solvent extract or a non-polar solvent extract as an active ingredient.

      The pharmaceutical composition for skin whitening containing the extract of the present invention as an active ingredient may further comprise suitable carriers, excipients and diluents conventionally used in the production of pharmaceutical compositions.

The pharmaceutical composition for skin whitening comprising the extract according to the present invention as an active ingredient can be administered orally or topically in the form of powders, granules, tablets, capsules, suspensions, emulsions, external preparations such as syrups and aerosols, And may be used in form of a cream, a gel, a patch, a spray, an ointment, a warning agent, a lotion, a liniment, a pasta or a cataplasm.

Examples of carriers, excipients and diluents that can be contained in the composition containing the extract of the present invention as an active ingredient include lactose, dextrose, sucrose, oligosaccharide, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, , Gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, it is preferable to use a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, etc. which is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose ), Lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate talc may also be used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions and syrups, and various excipients such as wetting agents, sweetening agents, fragrances, preservatives, etc. in addition to water and liquid paraffin, which are simple diluents commonly used . Formulations for parenteral administration may include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, suppositories, and the like. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. As a base for suppositories, witePSol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like can be used.

The composition for skin whitening comprising the extract of the present invention as an active ingredient can be used as an external preparation for skin which can be applied to the skin, and can be used as a cream, gel, patch, spray, ointment, warning agent, lotion, liniment, The present invention can be used as a pharmaceutical composition in the form of external preparation for skin, but is not limited thereto.

A preferable dosage of the composition containing the extract of the present invention as an active ingredient varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. However, for the desired effect, the composition of the present invention is preferably administered at 0.0001 to 100 mg / kg per day, preferably 0.001 to 10 mg / kg per day. External administration may be administered once a day or divided into several doses. The dose is not intended to limit the scope of the invention in any way.

      In another aspect, the present invention provides a skin whitening cosmetic composition comprising as an active ingredient an extract of Aspergillus oryzae, a polar solvent extract or a non-polar solvent extract.

      The extract of the present invention can be used variously in cosmetics and cleansers having a skin whitening effect. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, and skins, and cleansing, cleansing agents, soaps, treatments, and essences.

The cosmetic of the present invention may further comprise a component selected from the group consisting of water-soluble vitamins, oiliness vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

The water-soluble vitamin is not particularly limited as long as it can be compounded in cosmetics. Preferably, vitamin B, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, And their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbate-2-phosphate etc.) can also be added to water-soluble vitamins . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

Usable vitamins include vitamins such as vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) Alpha-tocopherol vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl laurate, and the derivatives thereof (palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbin, dl-alpha tocopherol acetate, Ether, etc.) are also included in the usable vitamins used in the present invention. Usability Vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The polymeric polysaccharide may be any compound as long as it can be incorporated in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

The seaweed extract may be any of those which can be compounded in cosmetics. Preferably, the seaweed extract is selected from the group consisting of algae extract, red pepper extract, green algae extract and the like. Also, the algae extract may be colored guanine, arginic acid, Potassium alginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.

The cosmetic composition of the present invention may contain, in addition to the above-mentioned essential components, other components usually added to the cosmetic composition.

Examples of the compounding ingredients that may be added include organic solvents such as a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorbent, a preservative, a bactericide, an antioxidant, a plant extract, a pH adjuster, A blood circulation accelerator, a cold agent, an antiperspirant agent, and purified water.

Examples of the oil retaining component include ester-based oil retaining, hydrocarbon-based oil retaining, silicone-based oil retaining, fluoric oil retaining, animal retention and plant retention.

Examples of ester-based fats include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, Butyl isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, isostearic acid isostearyl, isostearyl palmitate, octyldodecyl myristate, Trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic acid pentaerythritol tetra (2-ethylhexanoate) , Decyl caprylate, decyl laurate, hexyl laurate, myristate decyl, myristyl myristate, myristine monoethyl stearate, stearyl stearate, decyl oleate, ricinoleic acid tri Isopropyl palmitate, isostearyl stearate, isostearyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl stearate, isopropyl stearate, isopropyl stearate, Ethylhexanoate, stearyl 2-ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol, Propylene glycol dicaprylate, propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl glycol dioctanoate, glyceryl tricarboxylate, glyceryl triunsaturated acid, glyceryl triisopalmitate, glyceryl triisostearate, octyl dodecanoate Silane, octanoic acid, octanoic acid, octanoic acid, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, Octyldecyl lactate, octyldecyl lactate, octyldecyl lactate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisobutyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, But are not limited to, ethyl, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, But are not limited to, dioctyl sebacate, stearic acid cholesteryl, isostearic acid cholesteryl, hydroxystearic acid cholesteryl, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid pitostearyl, 12-stearoylhydroxystearic acid isostearyl, 12-stearoylhydroxystearic stearyl, 12-stearoyl One hydroxy stearic acid and the like esters such as cetearyl source.

Examples of the hydrocarbon hydrocarbon-based fats include hydrocarbon fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline.

Examples of silicone based oils include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstarchoxysiloxane copolymer, alkyl Modified silicone oils, and amino-modified silicone oils.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Examples of animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, , Corn oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, mead home oil, egg oil, , Canned wax, carnauba wax, liquid lanolin, hardened castor oil, and the like.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.

Examples of water-soluble low-molecular moisturizing agents include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Polypropylene glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like.

Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol and cholesterol ester.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. .

Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Examples of the nonionic surfactant include self emulsifying monostearate glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE (Polyoxyethylene / polyoxypropylene) copolymer, POE.POP alkyl ether, polyether-modified silicone, polyether-modified silicone, polyoxyethylene-polyoxypropylene (POE) Alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, Alkylsulfosuccinic acid salts, acylated hydrolyzed collagen peptide salts, and perfluoroalkyl phosphoric acid esters, and the like can be mentioned. have.

Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium chloride, Benzalkonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like.

Examples of the amphoteric surfactant include carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amide amine type Amphoteric surfactants and the like.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

As the organic powder, metallic soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Examples of ultraviolet absorbers include paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethyleneglycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate , Octyl methoxycinnamate, dioctyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl paratumoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino- p- (carbo-2'-ethylhexyl-1'- , 3,5-triazine, 2- (2- And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

Examples of the disinfectant include hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc filitione, benzalkonium chloride, No. 301, mononitro and eicol sodium, and undecylenic acid.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, any of the above-mentioned components may be blended within the range not to impair the objects and effects of the present invention, but it is preferably 0.01 to 5% by weight based on the total weight, More preferably from 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients conventionally used in cosmetic compositions in addition to the above-mentioned compounds, for example, conventional additives such as stabilizers, solubilizers, vitamins, And a carrier.

The cosmetic composition of the present invention can be prepared into any formulation conventionally produced in the art, and examples thereof include emulsions, creams, lotions, packs, foundations, lotions, essences, and hair cosmetics.

Specifically, the cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, Packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

      As described above, the composition of the present invention containing the extract of Aspergillus oryzae, the polar solvent extract or the non-polar solvent extract as an effective ingredient effectively inhibits tyrosinase activity, which is an enzyme essential for the melanin biosynthetic process, and the B16 mela And inhibits the biosynthesis of melanin in NORMA cells. Therefore, it can be effectively used as a whitening cosmetic composition and a pharmaceutical composition.

Hereinafter, the present invention will be described in detail with reference to the following examples and experimental examples.

However, the following examples and experimental examples are illustrative of the present invention, and the content of the present invention is not limited by the following examples and experimental examples.

Example  One. Bark tree  Fruit extract PS -1)

100 g of 70% ethanol was added to 1 kg of 70% ethanol, and the mixture was boiled for 3 hours in an extractor equipped with a condenser. The filtrate was filtered through a 400-mesh filter cloth, cooled to room temperature, and then dried in a rotary evaporator at 60 ° C. And concentrated under reduced pressure to obtain 48 g of the extract (PS-1).

Example  2 to 7. Bark tree  Fruit extract PS -2 to 7)

The results are shown in Table 1 below. The results are shown in Table 1 below. ≪ tb > < TABLE >

Sorghum fruit extract menstruum extract Example 2. PS-2 Purified water 31.2g Example 3. PS-3 30% ethanol 28.7g Example 4. PS-4 30% glycerin 21.2 g Example 5. PS-5 30% butylene glycol 25.1 g Example 6. PS-6 80% methanol 32.2 g Example 7. PS-7 30% propylene glycol 23.1 g

Example  8. Bark tree  Fruit extract PS -8)

30 g of the reduced pressure concentrate of Example 1 was dispersed in 300 g of distilled water, 300 g of hexane was added thereto, shaken, allowed to stand at room temperature until layer separation, and then the hexane layer was recovered. The hexane layer obtained by performing the same procedure three times was concentrated under reduced pressure using a rotary evaporator to obtain 2.12 g of powdery fraction (PS-8).

Example  9. Bark tree  Fruit extract PS -9)

300 g of ethyl acetate was added to the water layer of Example 8, shaken, allowed to stand at room temperature until layer separation, and then the ethyl acetate layer was recovered. The ethyl acetate layer obtained by the same procedure three times was concentrated under reduced pressure using a rotary evaporator to obtain 4.2 g of powdery fraction (PS-9).

Example  10. Bark tree  Fruit extract PS -10)

300 g of butanol was added to the water layer of Example 9, shaken, allowed to stand at room temperature until layer separation, and then the butanol layer was recovered. The same procedure was repeated three times. The butanol layer was concentrated under reduced pressure using a rotary evaporator to obtain 6.4 g of powdery fraction (PS-10).

Example  11. Bark tree  Fruit extract PS -11)

The residue layer of Example 10 was concentrated under reduced pressure to obtain 16.2 g of powdery fraction (PS-11).

Experimental Example  One. Tyrosinase  Active inhibitory effect

The inhibitory effect of tyrosinase activity on the extracts of the sorghum fruit of Examples 1 to 11 was measured.

1) Experimental method

The inhibitory activity of tyrosinase is generally measured spectroscopically. This experiment was carried out by Barney et al. (Vanni A. at al.Annali di Chimica,80 (35), 1990). Tyrosinase is an enzyme that is purified from potato or mushroom. In this experiment, mushroom extract was used. 1.0 ml of 0.1 M potassium phosphate buffer solution (pH 6.8), 1.0 ml of 0.3 mg / ml L-tyrosine aqueous solution and 0.1 ml of 1250 unit / ml mushroom tyrosinase (Sigma, USA) were mixed with 0.2 Ml, and enzyme reaction was carried out at 37 ° C for 10 minutes. Then, the absorbance of the reaction solution was measured at 480 nm (JASCO V-550, japan) and calculated according to the following equation (1) to determine the enzyme inhibition activity of the sample. As a positive control, 0.2 ml of arbutin (Bio Land) was used at the same concentration as that of the experimental group, and a reaction solution to which no sample was added was used as a control group in the experimental group. The results are shown in Table 2.

(Empirical formula 1)

Tyrosinase activity inhibition rate (%) = [(A-B) / A] x 100

       A: absorbance at 480 nm of the reaction solution to which no sample was added

B: Absorbance at 480 nm of the reaction solution to which the sample was added

2) Experimental results

extract 100 [mu] g / ml At a concentration of
Mushroom Tyrosinase  % Inhibition PS-1 37.23 PS-2 29.34 PS-3 28.56 PS-4 25.44 PS-5 29.30 PS-6 32.34 PS-7 31.24 PS-8 24.56 PS-9 40.21 PS-10 42.34 PS-11 29.46 Arbutin 47.28

In Table 2, the extract of Fagus cucumber extract of the present invention all showed that the inhibition rate of tyrosinase activity was over 24.56%, indicating that even a small amount thereof inhibits tyrosinase activity. Also, when compared with arbutin used as a control group, an equivalent effect was obtained. Therefore, the extract of Fagus cucumber extract of the present invention is excellent in the inhibitory effect of tyrosinase activity.

Experimental Example  2. B16-F1 Melanoma  Cell Cytotoxicity Measurement

The cell survival rate of B16-F1 melanoma cells (CRL-6323, ATCC) was determined by using the extracts and fractions of Aspergillus oryzae as a sample.

1) Experimental method

In the present invention, an MTT experiment was performed to measure cytotoxicity, and specific experimental methods were as follows.

B16-F1 melanoma cells, the 10% FBS (Fetal bovine serum, Gibco) was added a DMEM (Dulbecco's modified eagle's medium , Gibco) in 1 × 10 ⁵ density in 6 well plate 5 during the inoculation and day (Nunc) of cell % CO _2, and incubated at 37 ℃. Then, the cells were replaced with fresh DMEM supplemented with 10% FBS, and the samples were treated for different concentrations and cultured for 3 days. After 3 days, the medium was removed and 2 ml of a 0.25 mg / ml solution of MTT (3- [4,5-dimethylthiazole-2-yl] -2,5-diphenyltetrazolium bromide), Sigma) Lt; / RTI > Then, 2 ㎖ of DMSO (dimethylsulfoxide) was added to the cells from which the MTT solution had been removed to dissolve the MTT formazan, and the absorbance at 570 nm was measured. All experiments were repeated three times and statistical analysis was performed to obtain the average value. The results are shown in Table 3.

(Empirical formula 2)

Cell viability (%) = absorbance 570 (sample) / absorbance 570 (control) * 100

2) Experimental results

extract Cell survival rate  (%) PS-1 (5 [mu] g / ml) 101.23 PS-1 (10 [mu] g / ml) 99.56 PS-8 (5 [mu] g / ml) 98.24 PS-8 (10 [mu] g / ml) 96.65 PS-9 (1 [mu] g / ml) 101.03 PS-9 (5 [mu] g / ml) 98.56 PS-10 (1 [mu] g / ml) 104.25 PS-10 (5 [mu] g / ml) 102.87 PS-11 (1 [mu] g / ml) 103.57 PS-11 (5 [mu] g / ml) 100.89

As shown in Table 3, the cell survival rate is close to 100%, indicating that the extract of Fagus cucumber does not exhibit cytotoxicity.

Experimental Example  3. B16-F1 Melanoma  Measurement of inhibitory effect on melanin biosynthesis using cells

1) Experimental method

The melanogenesis inhibitory effect of B16-F1 melanoma cells was examined by Fukuda et al., J. Soc . Cosmetic Chem . , 42 (361) , 1991). The number of wells according to all concentrations was prepared in triplicate.

Specifically, B16-F1 melanoma cells were inoculated into a 6-well plate at a density of 1 × 10 ⁵ cells in DMEM supplemented with 10% FBS, and cultured at 37 ° C. in 5% CO ₂ for 1 day. Then, the cells were replaced with fresh DMEM supplemented with 10% FBS, and the samples were treated for different concentrations and cultured for 3 days. After 3 days, the cells were washed with 2 ml of PBS (phosphate buffer, saline), treated with 0.5 ml of 1N NaOH, and the cells were collected in a 1.5 ml tube to obtain intracellular melanin. The collected cells were transferred to a 96-well plate and the absorbance at 450 nm was measured to determine the amount of melanin per protein. As a control, arbutin (BioLand) at a concentration of 100 ug / ml was used, and as a control group, a reaction solution to which no sample was added was used.

Table 4 shows the inhibition of melanogenesis of the sorghum fruit obtained therefrom.

(Empirical formula 3)

Melanin production inhibitory effect (%) = [1-MCa / CVa] x 100

CVa: cell survival rate of the reaction solution treated with the sample

MCa: [(A-B) / A] x 100

A: absorbance at 450 nm of the reaction solution not treated with the sample

B: absorbance at 450 nm of the reaction solution treated with the sample

2) Experimental results

The extract (10 [mu] g / ml) Melanin production inhibitory effect (%) PS-1 24.65 PS-8 22.35 PS-9 20.48 PS-10 23.84 PS-11 29.74 Arbutin (100 [mu] g / ml) 20.40

As shown in the above table, the extract of Aspergillus oryzae of the present invention showed a high inhibitory effect on melanin formation even at a small amount of 10 占 퐂 / ml. In addition, these extracts exhibited the same or better effect than that of arbutin, which is known to have excellent whitening effect, when it was added with 100 / / ml of arbutin corresponding to 10 times the amount of the extract.

Formulation example  1. Flexible lotion ( skin )

As described below, a flexible lotion (skin) containing the extract of Liliaceae obtained in Examples 1 and 12 was prepared according to a conventional method.

number Raw material Reference Example  One Reference Example  2 Comparative Formulation Example One   PS-1 3.0 - - 2   PS-12 - 3.0 - 3 glycerin 3.0 3.0 3.0 4 Butylene glycol 2.0 2.0 2.0 5 Propylene glycol 2.0 2.0 2.0 6 Polyoxyethylene (60) curing  Castor oil 1.00 1.00 1.0 7 ethanol 10.0 10.0 10.0 8 Triethanolamine 0.1 0.1 0.1 9 antiseptic a very small amount a very small amount a very small amount 10 Pigment a very small amount a very small amount a very small amount 11 Spices a very small amount a very small amount a very small amount 12 Purified water Balance Balance Balance

Formulation example  2. Nourishing lotion (lotion)

As described below, a nutritional lotion (lotion) containing the extract of Liliaceae extract obtained in Examples 1 and 12 was prepared according to a conventional method.

number Raw material Reference Example  One Reference Example  2 Comparative Formulation Example One   PS-1 3.0 - - 2   PS-12 - 3.0 - 3 Sitosterol 1.70 1.70 1.70 4 Polyglyceryl 2-oleate 1.50 1.50 1.50 5 Setares-4 1.2 1.2 1.2 6 cholesterol 1.5 1.5 1.5 7 Dicetyl phosphate 0.4 0.4 0.4 8 Concentrated glycerin 5.0 5.0 5.0 9 Sunflower oil 10.0 10.0 10.0 10 Carboxyvinyl polymer 0.2 0.2 0.2 11 Xanthan gum 0.3 0.3 0.3 12 antiseptic a very small amount a very small amount a very small amount 13 Spices a very small amount a very small amount a very small amount 14 Purified water Balance Balance Balance

Formulation example  3. Nourishing Cream

As described below, nutritional creams containing the extract of Liliaceae obtained in Examples 1 and 12 were prepared according to a conventional method.

number Raw material Reference Example  One Reference Example  2 Comparative Formulation Example One   PS-1 5.0 - - 2   PS-12 - 5.0 - 3 Sitosterol 4.0 4.0 4.0 4 Polyglyceryl 2-oleate 3.0 3.0 3.0 5 Setares-4 2.0 2.0 2.0 6 cholesterol 3.0 3.0 3.0 7 Dicetyl phosphate 0.4 0.4 0.4 8 Concentrated glycerin 5.0 5.0 5.0 9 Sunflower oil 22.0 22.0 22.0 10 Carboxyvinyl polymer 0.5 0.5 0.5 11 Triethanolamine 0.5 0.5 0.5 12 antiseptic a very small amount a very small amount a very small amount 13 Spices a very small amount a very small amount a very small amount 14 Purified water Balance Balance Balance

Formulation example  4. Essence

As described below, essences containing the extract of Liliaceae obtained in Examples 1 and 12 were prepared according to a conventional method.

number Raw material Reference Example  One Reference Example  2 Comparative Formulation Example One   PS-1 5.0 - - 2   PS-12 - 5.0 - 3 Sitosterol 1.70 1.70 1.70 4 Polyglyceryl 2-oleate 1.50 1.50 1.50 5 Setares-4 2.0 2.0 2.0 6 cholesterol 3.0 3.0 3.0 7 Dicetyl phosphate 0.4 0.4 0.4 8 Concentrated glycerin 5.0 5.0 5.0 9 Sunflower oil 22.0 22.0 22.0 10 Carboxyvinyl polymer 0.5 0.5 0.5 11 Triethanolamine 0.5 0.5 0.5 12 antiseptic a very small amount a very small amount a very small amount 13 Spices a very small amount a very small amount a very small amount 14 Purified water Balance Balance Balance

Formulation example  5. Foundation

As described below, a foundation containing the Lilac extract obtained in Examples 1 and 12 was prepared according to a conventional method.

number Raw material Reference Example  One Reference Example  2 Comparative Formulation Example One   PS-1 3.0 - - 2   PS-12 - 3.0 - 3 Wax 2.0 2.0 2.0 4 Cyclomethicone 2.0 2.0 2.0 5 Liquid paraffin 5.0 5.0 5.0 6 Squalane 5.0 5.0 5.0 7 Stearic acid 2.0 2.0 2.0 8 Lipophilic monostearic acid glycerin 3.0 3.0 3.0 9 Caprylic / capric triglyceride 4.0 4.0 4.0 10 glycerin 4.0 4.0 4.0 11 Propylene glycol 3.0 3.0 3.0 12 Butylene glycol 3.0 3.0 3.0 13 Triethanolamine 1.0 1.0 1.0 14 Aluminum magnesium silicate 0.5 0.5 0.5 15 Pigment 12 12 12 16 antiseptic a very small amount a very small amount a very small amount 17 Spices a very small amount a very small amount a very small amount 18 Purified water Balance Balance Balance

Formulation example  6. Hydrophilic ointment

As described below, a hydrophilic ointment containing the Lilac extract obtained in Examples 1 and 12 was prepared according to a conventional method.

number Raw material Content (% by weight) Content (% by weight) One   PS-1 0.5 - 2   PS-12 - 0.5 3   White vaseline 250 250 4   Stearyl alcohol 220 220 5   Ethyl (or methyl) p-oxybenzoate 0.25 0.25 6   Propylene glycol 120 120 7   Sodium lauryl sulfate 15 15 8   Propyl p-oxybenzoate 0.15 0.15

clinical Experimental Example 1 . Skin whitening effect

To test the effect of the extract of Aspergillus oryzae on skin whitening, Lotion of Formulation Example 1 (Test group) and Comparative formulation (control group) of Formulation Example 2 was applied to 19-22 year old women with healthy skin After the induction of pigmentation, the skin whitening effect of the extract was investigated by using the double blind method and the randomized human body test method for 6 weeks.

clinical Experimental Example  1-1. Visual evaluation

The skin whitening effect was evaluated before the use of formulations and at 1 week, 2 weeks, 4 weeks, and 6 weeks after use. The evaluation criteria were evaluated by two observers using the 1-7 scale (Tables 11 and 12). The test group and the control group were repeatedly evaluated according to the viewpoints, and the test results were repeatedly measured by using the variance analysis method (p <0.05).

Visual evaluation by observer 1 Group    week N Evaluation value S.E.Mean S.D. Test group 0 weeks 20 6.2600 0.1413 0.5771 1 week 20 5.8500 0.1883 0.8760 2 weeks 20 4.9100 0.1566 0.7552 4 weeks 20 4.1700 0.1964 0.8125 6 weeks 20 3.5400 0.2158 1.0250 Control group 0 weeks 20 6.4500 0.1639 0.6982 1 week 20 6.0200 0.2112 0.9267 2 weeks 20 5.2300 0.1837 0.8891 4 weeks 20 4.5500 0.2019 0.9351 6 weeks 20 4.0300 0.1879 0.8874

Visual evaluation by observer 2 Group    Week N Evaluation value S.E.Mean S.D. Test group 0 weeks 20 6.5900 0.1422 0.6906 1 week 20 6.3300 0.1400 0.6908 2 weeks 20 5.2900 0.1420 0.6808 4 weeks 20 4.4800 0.15438 0.6182 6 weeks 20 3.4200 0.1778 0.7292 Control group 0 weeks 20 6.4500 0.1452 0.6388 1 week 20 6.5900 0.1427 0.6270 2 weeks 20 5.6800 0.1576 0.6459 4 weeks 20 5.3600 0.1388 0.6827 6 weeks 20 4.3600 0.1672 0.6606

The results of the clinical tests showed that the addition of formulations of P. vivax extract showed significant improvement compared to the untreated control group.

clinical Experimental Example  1-2. Color difference meter  Measurement of melanin index using

The degree of skin color improvement was evaluated by using a colorimeter (MPA-580) before and after 1, 2, 4, and 6 weeks of use of the test group and the control group. The results of evaluating the melanin index at each time point are shown in Table 13.

The melanin index of the test and control groups Group    Week N Melanin index S.E.Mean S.D. Test group 0 weeks 20 202.97 9.0743 43.1593 1 week 20 201.12 9.0506 38.2643 2 weeks 20 196.32 8.8677 39.3158 4 weeks 20 187.97 8.3594 36.0426 6 weeks 20 173.532 7.8700 30.6719 Control group 0 weeks 20 211.87 8.8775 40.2956 1 week 20 205.88 8.7292 37.2059 2 weeks 20 203.78 7.7896 32.5386 4 weeks 20 198.50 7.9256 31.6866 6 weeks 20 195.40 7.7875 32.6765

The melanin index of each test group was decreased compared to the control group.

Claims (12)

Platycarya strobilacea ) fruit extract as an active ingredient. Platycarya strobilacea ) fruit extract as an active ingredient. 2. The cosmetic composition for whitening skin according to claim 1, wherein the extract comprises 0.01 to 50% by weight based on the total weight of the composition. [Claim 2] The skin whitening extract of claim 1, wherein the extract is a crude extract extracted with at least one solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, butylene glycol and glycerin. / RTI &gt; [Claim 5] The cosmetic composition for skin whitening according to claim 4, wherein the crude extract comprises a soluble extract obtained by extracting the solid content of the crude extract with at least one solvent selected from the group consisting of hexane, ethyl acetate, butanol and water. The cosmetic composition for skin whitening according to claim 1, wherein the composition has a skin whitening effect by inhibiting tyrosinase activity or melanin production. [Claim 3] The composition for skin whitening according to claim 2, wherein the extract comprises 0.01 to 50% by weight of the extract. [Claim 3] The skin whitening extract of claim 2, wherein the extract is a crude extract extracted with at least one solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, butylene glycol and glycerin. &Lt; / RTI &gt; [Claim 9] The pharmaceutical composition for skin whitening according to claim 8, wherein the crude extract comprises an extract obtained by extracting a solid content of the crude extract with at least one solvent selected from the group consisting of hexane, ethyl acetate, butanol and water. The pharmaceutical composition for skin whitening according to claim 2, wherein the composition has a skin whitening effect by inhibiting tyrosinase activity or melanin production. The composition according to claim 1, wherein the composition is at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritional cream, a moisturizing cream, a hand cream, Pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion or body cleanser. The pharmaceutical composition according to claim 2, wherein the composition is a cream, a gel, a patch, a spray, an ointment, a warning agent, a lotion, a liniment, a pasta or a catarcoma.