KR101456062B1 - Cosmetic composition for preventing of kin trouble - Google Patents

Abstract

The present invention relates to a cosmetic composition for alleviating skin troubles, and more particularly, to a cosmetic composition for alleviating skin troubles comprising a fermented extract of Rhodiola as an active ingredient. The cosmetic composition for relieving skin troubles according to the present invention is safe for the skin because of its excellent anti-inflammatory effect, which is a skin tear-reducing effect.

Description

[0001] The present invention relates to a cosmetic composition for preventing skin trouble,

The present invention relates to a cosmetic composition for relieving skin troubles, and more particularly, to a cosmetic composition for relieving skin troubles which is excellent in anti-inflammatory effect, which is a skin troubles reducing effect, and is safe for skin.

Cosmetics are products used to protect the skin and beautify and clean the skin, but the composition thereof inevitably includes ingredients that are different from the purpose of skin protection. These ingredients include surfactants, preservatives, fragrances, sunscreens, pigments, as well as various ingredients for imparting other efficacy and effects. These ingredients are generally known to cause skin irritation, rashes, swelling and other problems (Maibach, H., I. Contact Dermatitis, 6. 369-404, 1980). In addition, sebum and sweat discharged from the body, fatty acids in cosmetic ingredients, higher alcohols, protein components and the like may be decomposed into substances having high toxicity by the skin-on-ground bacteria existing on the skin, It is well known that skin inflammation is caused by ultraviolet rays from the sun.

Inflammation reactions are manifested in five phenomena, such as feeling of redness, pricking feeling, burning hotness, swelling, and tissue changes, which may be caused by harmful environmental conditions, It is a physiological reaction to protect a living body from intrusion and mechanical damage. These inflammatory phenomena lead to a large number of polymorphonuclear leukocytes (PMNs) and immune substances, and these increased cells secrete a variety of inflammatory cell products, proteases and cytokines. I can do it. However, this action may cause harmful damage to adjacent tissue cells and non-cellular components. Therefore, under proper conditions, the normal function is restored after the initial state of inflammation. However, if irritant stimulating agents are not eliminated or continuously produced, chronic inflammation occurs as a result, resulting in more serious tissue damage.

Atopy and acne, forms of skin troubles related to inflammation, are described as follows.

Atopy is characterized by severe itching. When it irritates the skin, the itching becomes worse, and when it is scratched more and more, it becomes scratched, and the bacterial infection (Staphylococcus aureus, Streptococcus etc.) occurs and inflammation occurs. One of the important factors in the problem of atopy is the secondary infections and superantigen toxins that occur at this time. 80-90% of atopic patients are infected with Staphylococcus aureus and Streptococcus pyogenes in their skin, where Anit-Staphylococcus IgE is produced, which causes the superantigen toxin And it may cause deterioration. The superantigen toxin of Staphylococcus aureus selectively stimulates T lymphocytes through antigen presenting cells in Langerhans cells to produce interleukin-1 and TNF-alpha, and T-cell stimulated by these cells produces various intracellular cytokines And it causes inflammation. In other words, the superantigen toxin promotes the secretion of interleukin-4 and interleukin-5, thereby inhibiting the secretion of IFN-r and increasing the number of CLA, thereby promoting the production of IgE.

Second, patients infected with Staphylococcus aureus secrete histamine from the basophil by the IgE antibody to the staphylococcus toxin, which leads to worsening of atopic dermatitis.

Therefore, the use of moisturizers to prevent skin dryness is frequently recommended, and secondary infection and its control of superantigen factors are very important. In other words, atopy appears to be a basic pathogenesis of genetic defects, but it is triggered by various external factors.

The causes of atopic eczema include genetic factors, sensitive and dry skin, metabolic factors, excessive production of IgE, abnormalities of the autonomic nervous system, psychological factors, and irritating factors.

Atopy is a disease whose mechanism is not simple. From an immunological viewpoint, it is not an immunological problem but a problem of various patterns. For example, the problem of gamma interferon, which inhibits excessive production of IgE, may occur due to the fact that its production is lower than the average value, but it is smaller than interleukin-4, which has opposite action, And even if it is normal in terms of quantity or quality, sensitivity to the stimulation of interferon may be reduced.

On the other hand, acne can be divided into inflammatory acne lesions and non-inflammatory (acne vulgaris) acne lesions depending on symptoms.

Inflammatory acne lesions cause sebaceous matter to escape from cottonseed and cause inflammation reaction to surrounding skin tissue, which is red due to inflammation. Squeezing or pressing the acne buds will cause the cotton buds to burst into inflammatory acne lesions, which increases the risk of leaving acne scars.

Basically, there are three types of inflammatory acne lesions: papules, pustules, and nodules.

The acne acne (red acne) is as follows. The term "papule" refers to a small, firm red lesion that sometimes appears as an intermediate form of non-inflammatory acne lesions and acne lesions, and is accompanied by mild inflammation, so the hue appears red, . So, the acne that has sprung out is called acne acne. This is an inflammatory acne, as described above, which varies in size, hardness, and degree of lightness depending on the degree of inflammation. The more inflammation, the bigger, the more vivid and the longer it tends to go. Less irritated ones are just a few (about 7%) just fading, but most of the time they become more inflamed or become acne acne.

The pustular acne (acne acne) is as follows. It is like a battlefield after a fierce battle. In other words, there are some bacteria, dead tissue, and white blood cells that collect bacteria to get rid of the bacteria. Pustular pustules are small and round in shape, similar to papules, but they are different from papules in that they contain paprika. There is no bacteria in bacteria such as bacteria, and it is the result of inflammation reaction by chemical stimulation by ingredients such as free fatty acid in sebum. Among the acne acne, small, harder ones around are faster than the larger, harder ones.

Next, nodular acne is the most severe form of acne and is large in size, inflammatory, and contains a large amount of agar. The nodules form the nectar by inducing the immune response to the skin tissue around the nectar from the contents of the cotton. Nodular acne is relatively deep in the skin and can be accompanied by pain, and is more likely to leave an acne scar during the healing process than other acne lesions.

The core substance of these various inflammatory processes is arachidonic acid, which is converted from cell membrane lipids by an enzyme called stimuli-activated phospholipase A2 (PLA2), and arachidonic acid is converted to cyclooxygenase (Prostaglandins), leukotrienes (Leukotrienes) via mediators of inflammation (Thomas, R., et al., &Quot; COX ") or lipoxygenase Rev. Physiol. Biochem. Pharmacol., 100, 1984).

Recently, researches on methods to reduce skin irritation and inflammation have been actively carried out. As a result of the researches so far, neural peptides such as substance P (Substance P) Kinases such as bradykinin activated by plasma, or various cytokines, prostaglandin E2 (hereinafter abbreviated as "PGE2") formed by the COX pathway, are involved in edema formation and vasodilation, Therefore, neuropeptide antagonists such as substance P, a bradykinin antagonist and a COX inhibitor have been proposed as anti-inflammatory agents, but the known anti-inflammatory agents have been proposed to be included in the product in terms of safety against skin And its use is limited because it has most problems in terms of stability.

On the other hand, fermentation is a process of decomposing organic matter using enzymes possessed by microorganisms. Fermentation and corruption proceed by a similar process, but decomposition is called fermentation when substances that are useful for our lives are made, and when they make odorous or harmful substances, it is called corruption. Therefore, if the herb medicine is fermented using microorganisms, the toxicity of the herb medicine is reduced, the low molecular weight is easily digested, and the transdermal absorption upon application to the skin becomes easy, and the bioavailability is improved.

The herbal medicines themselves can exhibit pharmacological effects, but the pharmacological effects can be further strengthened by fermentation or harmful components can be produced by the metabolism of microorganisms, so that a new pharmacological effect can be exhibited which is not present in the herbal medicine before fermentation.

Accordingly, it is an object of the present invention to provide a cosmetic composition for relieving skin troubles which is excellent in anti-inflammatory effect, which is a skin troubles reducing effect, and is safe for skin.

Another object of the present invention is to provide a cosmetic composition for alleviating skin troubles which further enhances the anti-inflammatory effect by further strengthening the pharmacological effect of each medicinal substance through fermentation.

In order to accomplish the above object, the present invention provides a method for producing a cucurbitaceous mushroom of the present invention, comprising the steps of: (1) preparing a cucurbitaceous mushroom, A composition for relieving skin troubles, which comprises as an active ingredient, a fermented extract of a medicinal substance selected from the group consisting of guinea pigs, red ginseng, ginseng, tea ginseng, chrysanthemum, chrysanthemum, and phylloquinone.

The fermentation extract can be obtained by adding a medicinal substance to yeast, lactic acid bacteria or bifidobacteria and culturing the same.

The fermented extract can be obtained by inoculating fungi into a medicinal material and solid fermentation.

The medicinal fermentation extract is preferably contained in the cosmetic composition in an amount of 0.0001 to 10% by weight.

The cosmetic composition may be formulated into ointment for external use, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like.

The cosmetic composition for relieving skin troubles according to the present invention is excellent in skin-relieving effect and persistence of effect by enhancing the pharmacological effect of each medicinal substance through fermentation while enhancing the anti-inflammatory effect.

Hereinafter, the cosmetic composition for skin troubles of the present invention will be described in detail.

The medicinal plant extracts described in the present invention can be used as medicinal plant extracts of the present invention for the production of medicinal plants such as gingkohwon, samsang, mushroom, mushroom, green tea, mushroom, mushroom, mushroom, Refers to an extract obtained by extracting a medicinal substance selected from at least one species selected from red ginseng, ginseng, tea ginseng, sorghum, cherry, and hoiothrix according to a conventional method. Also, the medicinal fermentation extract means a secondary extract obtained by inoculating and culturing the above-mentioned medicinal plant extract into yeast, lactic acid bacteria or bifidus microorganisms, or obtained by solid fermentation by adding fungi to commercially available medicinal products or pulverized products.

The inventors of the present invention have conducted studies to develop a fermented Chinese medicinal herb having excellent skin trouble abatement function, and found that it is possible to produce a fermented Chinese medicinal herb having excellent skin relief function, , Fermented broth, saururus, sambucola, cherry, yugi, chow, zucchini, chalcopyrum, chrysanthemum, chrysanthemum, and phloem were found to be superior to the extracts of the respective extracts in skin troubles. .

The cosmetic composition for relieving skin troubles according to the present invention is characterized in that it comprises at least one selected from the group consisting of gold, , Fermented extracts of a medicinal herb selected from the group consisting of red ginseng, ginseng, tea ginseng, sorghum, kaiji, and hoeicho as effective ingredients.

The above-mentioned Lonicera japonica is said to be a gold flower, a stalks and so on, and has the efficacy of cyanosis and detoxification, and treats fever, dufehrguff, whdeher, vigor, mumps and the like.

The ginseng (Adenophora tetraphylla) is a root of perennial herbaceous perennial herbaceous plants belonging to the genus Chrysanthemum, and has the effect of entering into the lungs, reclaiming the sound, and eliminating the waste heat. The root extract has a tonic, hemorrhagic, and detoxifying effect. It acts mainly on the menopause, clearing the lungs, removing sputum and stopping thirst. It is also effective in treating boils. It has the effect of helping to see the middle stage (middle 气) and harmonizing the stomach by generating the essence, so it regulates weakness of the spleen and stomach, and it is also effective in the loss of appetite. It also improves symptoms of diarrhea and stiffness in the stool. It is also effective in the symptoms of swelling of the chest, breathing, swelling of the face. In particular, overuse damage, cold sweating and thirst are applied to severe symptoms, the treatment is excellent.

The Houttuynia cordata is a perennial herb having a height of 30 to 50 cm and is used for a variety of inflammatory diseases such as urethritis, cystitis, uterine inflammation, pneumonia, sinusitis, bronchitis, It is also effective in hypertension, strong detoxification, and has an effect of lowering the blood glucose level of diabetes.

The Schizophyllum commune is 1-3 cm in diameter and has no stem. It attaches to the body as part of the side or back side of the gymnosperm, and it has a fan shape or a round shape, sometimes cracked like a palm. The surface is rough-haired and white-gray or gray-brown. The wrinkles are white-gray or light salmon colored or purple, and the margins are divided vertically and appear to overlap two sheets. The flesh is leather, and when it is dried, it is cured. When it is immersed in water, it becomes undone. It is known that the polysaccharide originating from the skirt mushroom has a healing effect on burns and wounds.

The green tea (Camellia sinensis) is produced by heating and moisture-evaporating young leaves of an unfermented tea leaf. From prevention of adult diseases such as hypertension and diabetes to dieting, the efficacy of green tea is unrivaled.

The abovementioned mushroom (Phellinus linteus) is a mushroom which grows in the mulberry stem. Recently, it has been reported that Japanese mushrooms contain high quality polysaccharides and proteins in large amounts, and that they have anticancer effects and tumor suppression rates. In addition to reports of more than 90% of cases, there is a report that it is particularly effective for gastric cancer "gastric cancer, esophageal cancer, duodenum cancer, colon cancer, rectal cancer and liver cancer".

The Perilla frutescens is called Korean ornamental herbaceous plant, and it is an annual plant of the flower family. It grows in many parts of Korea and grows in the field. The stem is square, and leaves and flowers resemble perilla. But it is different from perilla in that stems and leaves are purple. Joso hears cold, he is cold, choked by all body, runny nose, chest tight and thirsty when the chestnut leaves 40 ~ 50 g a month to drink, sweating and sweating after he gets better. It is also used in a variety of diseases such as cough, phlegm, sore throat, indigestion, swelling, athlete's foot, insomnia, paralysis, diabetes and back pain.

Broussonetia papyrifera is a fruit of cucumber tree which is a deciduous subordinate belonging to the genus Phalaenopsis. It is a medicinal substance which has a function to play a role in three books such as liver, rain, and god. Mainly, it strengthens the kidneys more and increases the tone of the eyes. Symptoms are treated by treating the symptoms of the liver and joints with weakness of the kidneys, symptoms of weakness in the liver, symptoms of pain in the eyes, and headaches and dizziness. It exhibits good efficacy in treating the species by lowering the ability to regulate moisture in the spleen. I can not see my eyes when I'm tired, I feel like I have something on my eyes, I have erectile dysfunction, I have back pain and knee pain.

The Coptis chinensis is the root of the mulberry tree. Yellow roots are said to be one after another. Nowadays, larvae of buttercups are mainly communicated, and berberine is known to be similar. Anti-inflammatory, sedative, nervous anxiety, hyperemia and inflammation. It is also used for bad digestion and gastritis due to bitter taste. Also, in the inflammation of the eyes, wash the antler with a moon figure.

The Pueraria thunbergii is a perennial vine plant with soybean. It grows wild and grows in the mountains, reaching vines, and has a reddish-brown flower from summer to autumn. It is often called the "Puerariae", which is dried in the sun by slicing the thick roots. Roots and flowers are used for fever, sweating, fever, fever, fever, fever, thirst and headache. When head and neck are sick with a cold, it affects tonsillitis, acute otitis media, rhinitis, It is also used to relieve pain and to heal wounds. Leaves are used for headaches and diuretics that come with hypertension. It is combined with Shiho Golden Plaster to heal the symptoms of pulling the neck in the headache without sweating in the cold.

The Magnolia kobus is a dried bud. Essential oils (citral, oenicol, chineol, caviarol), pinene and caproic acid are included in the bud. It is used for headache, nasal congestion, sinusitis, rhinitis, dizziness and cold. It is good for refreshing mood and improving memory.

The Equisetum arvense is a herbaceous plant that is commonly found in rice paddies and corridors but contains many pharmacological ingredients and can be used for similar purposes. Moon mold has diuretic, anti-inflammatory, hemostatic, lead poisoning lead poisoning. In addition, it has been known that there is no significant adverse effect and there is a pronounced diuretic effect in clinical use in cardiac dysfunction and edema.

Hedyotis diffusa is a perennial herbaceous plant belonging to Madagascar. It grows up to 30 cm tall and has a white flower. It grows like leaves of a white serpent 's serpent. It has a function to lower fever, to poison, to reduce inflammation, to urinate well, to make blood circulate and to suppress pain.

Belamcanda chinensis is a perennial herb with an iris of about 50-100 cm in height. The flowers are round, with black spots on the orange background, leopard pattern, and leaf shape like fan. The rhizome stretches sideways, the leaves are embraced by each other on both sides, and spread out like a fan in a flat manner on both sides. In Oriental medicine, throat medicine, cough medicine, throat is sore or sore. It can be eaten dalyeoseo, or put a piece of rootstock on the leaves to suck the concern. It mixes with the mountain beetroot (new sand vine rootstock) and rolls it into the mouth and blows it into the mouth. In the private sector, it has been proven to be effective as an analgesic drug for fever, sweating, diuretic drugs, back pain, rheumatism, gastritis, toothache, stab wounds and dislocations, poisonous poisonous or snakebite poisoning, .

The Saururus chinensis is a perennial herb which has a height of 15 to 30 cm from Saururus chinensis. The heart-shaped leaf grows and the yellow flower grows on the stem upper part. Below it, four white calyxes run in a cross. It is used for anti-inflammatory drug, diuretic, antidote, gonorrhea, urethritis, cystitis, hemorrhoids, pneumonia, bronchitis, bitter athlete's foot, athlete's foot, Drinking like a car every day, you can prevent arteriosclerosis. It also eats drowsiness and sinusitis.

The Inula Britannica is a perennial grass of Asteraceae with a height of 30 ~ 60 ㎝. It grows in the foothills of various places, the field. In Hanchi, the medicine to pee the flower, the medicine to cough, the medicine to cough, the cough and the chest with the placebo are frustrating and sick, and the revenge is used for the tea. In the private sector, roots are used as hemostatic drugs and inflammatory drugs, and it is effective to use the supernatant fluid for hemorrhoids.

The cherry (Prunus tomentosa) is a deciduous shrub belonging to the Rosaceae and has a height of about 3 m. Leaves are oval and have serrations. In the private sector, the roots are used as a medicine to eliminate chikusa and roundworms. Leaves are also used as a medicine to relieve snake venom.

Ulmus davidiana is written in Chinese characters such as elm oil or elm, and its shell is called 楡 皮 and root shell is called 楡 根 皮. According to the Introduction to Medicine, there is no poison, it makes the water available, it annihilates the tumor, and communicates the feces. It has also been used as a swelling medicine since it has been known to remove the heat and heat of the stomach and intestines, to treat insomnia and cure it. Phosphorus medicine believed that the root of the root of the elm tree was swollen than the elm and calmed down the pain. It was used together with bamboo salt to treat various diseases caused by inflammation.

The Pinus densiflora is also called Chuangyok Mok, a sparrow named because it is found mainly in Chunyang area. The vagina is firm and grows vertically, like a larch.

The Anemarrhena asphodeloides is a perennial plant of Liliaceae. It is about 60 ㎝ in height, and it takes root in autumn, polishes the root, and dries it in the sun. In respiratory diseases (when the lungs are hot or sweaty and the mouth is dry), pulmonary tuberculosis, coughs caused by pneumonia, urinary incontinence due to cystitis, and nocturnal fever, diarrhea, sedation, coughing, , And infectious diseases with fever.

Plantago asiatica is a perennial herbaceous plant with a plant height of about 50 cm. In June-July, a flower stalk emerges between the leaves, and a light-colored, small flower blooms with an acorn. It is said that it is said to be a lot of follow along the road mark of the wheel of the road. The seed is called a car. The tea electrons are put into prescription for the purposes of fever reduction, diuretic, Jinhae geomatics, and nervous stability. It is in the market that it is marketed as a health supplement food at a high price by developing the shell of the tea electron as a laxative and a succulent remover. It can be used for similar purposes by blasting tea electrons.

Xanthium sibiricum is an annual plant of Asteraceae about 1 to 1.5 m in height. Leaves are stitched together, male flower is attached to the tip of stem, female flower is beneath it. It grows in a field of various places, gil. The fruit is elliptical and has thorns. In autumn, pick the dried fruit and dry it. The fruit has a lactone compound which has anti-inflammatory action, drainage action, insecticidal activity against molds, and has a radiant effect. Root has diuretic action, metabolism promotion action, etc., and alkaloid component of root has anticancer activity.

The mold (Schizonepeta tenuifolia) is a herbaceous herb with a height of about 60 to 80 cm and an annual herb. It is used as an antipyretic medicine that sweats in one's mouth, a cold medicine as an antipyretic medicine, it is used when it feels hot, it hurts a head, it is used for poison of various scars, sore throat,

The Saxifraga stolonifera is said to be a rockfish because it grows well in a rocky borehole in the forest, and the appearance of a densely soft hairs on a young leaf resembles a tiger's ears, It is also known as the flower of the capital letter when the flower resembles the large flower of the Chinese character [大]. Hoi chi (虎 耳草) to eliminate wind, fever, cleanse blood and has the effect of releasing poison. Rubella, eczema, otitis media, solitary, cough, sputum, cough, symptoms of bleeding, pneumonia, hemorrhoids.

The medicinal materials may be prepared by a conventional method, for example, by extracting with an organic solvent such as distilled water, anhydrous or low-boiling alcohol having 1 to 4 carbon atoms, concentrating under reduced pressure and then drying to obtain a first extract . Specifically, there are various kinds of mushrooms, such as gold eums, samsam, mushroom, mushroom, green tea, mushroom, mushroom, mushroom, chrysanthemum, , Chisel, chisel or hoi choy were purchased and finely pulverized and pulverized in an extractor with a reflux condenser in an organic solvent such as 5 to 20 volumes of water or 1 to 4 carbon atoms anhydrous or hydrated lower alcohol with respect to the dry weight of the pulverized material Heat the extract at ~ 100 ° C for 1 to 5 hours to extract the extracts of the medicinal products. After filtration with a filter cloth, the residue is further extracted one or more times by the same method. The extracts are combined, concentrated under reduced pressure, and lyophilized or spray dried to obtain a dry extract.

The herbal extracts obtained as described above may be added to yeast, lactic acid bacteria or bifidus bacteria and cultured to obtain a final fermented extract. At this time, it is preferable that the medicinal plant extract is added to the basic medium of yeast, lactic acid bacteria or bifidobacteria in an amount of 1 to 20% by weight. In addition, the pharmaceutical fermentation extract may be obtained by inoculating the commercially available medicinal product itself or a slice or powder into a fungi, followed by solid fermentation.

The yeast may be Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids , Saccharomyces boulardii or the like.

The lactic acid bacteria may be selected from the group consisting of Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus confusus, Lactobacillus fermentum and Lactobacillus brevis .

Examples of the bifidobacteria include Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum , Bifidobacterium infantis , and the like.

The fungus can be a medicinal mushroom mold such as Inonnotus-obliquus, Cordyceps sinensis, Paecilomyces japonica , Tricholoma matsutake, phellinus linteus and Ganoderma lucidum. have.

More specifically, when yeast is used as a microorganism, it has a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL), such as gonghwa, samsam, mushroom, skimmer mushroom, green tea, mushroom, Yeast was inoculated into a primary culture medium (YM medium) containing primary extracts of Munhyeong, Baekwha, Sambilac, Sambuche, Laotchia, Chrysanthemum, Chrysanthemum, 5.5 to 6.5, at a temperature of 25 to 35 占 폚, and aeration amount of 0.05 to 0.5 VVM for 30 to 120 hours. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the yeast body, and the concentrate is concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser, followed by lyophilization, thereby obtaining each of the fermentation extracts of medicinal plants.

When lactobacillus is used as a microorganism, it has a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL), and it has a low concentration of about 10 ⁵ to 10 ⁷ cells / mL in the order of ginseng, ginseng, (MRS medium) containing the first extracts of Phellinus linteus, Sambucoptera, Sambucongeae, Cherry, Chrysanthemum morifolium, Chrysanthemum, Zygomycetes, The culture is carried out for 24 to 120 hours while maintaining the temperature of 35 to 38 DEG C and the aeration amount of 1 VVM. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the lactic acid bacterium, and the concentrate is concentrated under reduced pressure at 60 ° C in a distilling apparatus equipped with a cooling condenser, followed by freeze-drying, thereby obtaining each of the fermentation extracts of medicinal plants.

When bifidobacteria are used as microorganisms, they have a constant concentration (about 10 ⁵ to 10 ⁷ cells / mL) and have a specific concentration (about 10 ⁵ to 10 ⁷ cells / mL) of gold, silver, Bifidobacteria were inoculated on a primary culture medium (BL medium) containing primary extracts, anthracnose, antimicrobial agent, Sambucoptera, Sambuca, Cherry, 7.5, temperature 35 to 38 캜, and incubated for 24 to 120 hours while maintaining anaerobic culture conditions. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and bifidus microbial cells, and the suspension is concentrated in a distillation apparatus equipped with a cooling condenser at 60 ° C and freeze-dried to obtain the respective fermentation extracts of medicinal products.

In addition, when fungi is used as a microorganism, it is possible to use fungi such as gold euwha, samsam, osengcheon, skirt mushroom, green tea, mushroom, persimmon, Fungus is inoculated in each of 1 to 10% by weight in each of the crushed, ground, crushed, dried, ground, chiseled, chiseled, Day solid fermentation to obtain each of the medicinal fermented extracts.

The pharmaceutical fermented extracts as described above exhibit a superior anti-inflammatory and anti-inflammatory effect when compared with common medicinal plant extracts when the anti-inflammatory effect is measured by the PGE2 generation inhibitory effect test. In addition, when the cosmetic composition is manufactured using the above-mentioned fermentation extracts of medicinal materials, it is safe to the skin without any side effects when applied to human skin and exhibits an excellent skin troubles reducing effect.

The medicinal fermentation extract obtained as described above is preferably contained in an amount of 0.0001 to 10% by weight, more preferably 0.0005 to 10% by weight, in the cosmetic composition for skin troubles of the present invention. When the content is less than 0.0001% by weight, no remarkable effect can be expected. When the content is more than 10% by weight, there is no evidence of a marked effect due to the increase of the content.

The formulation of the cosmetic composition for relieving skin troubles containing the above fermented extract of the present invention as an active ingredient is not particularly limited and may be appropriately selected according to the purpose. For example, the cosmetic composition of the present invention may be formulated into ointment for external use, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like. In this case, the ointment for external use on skin may be prepared so as to contain 50 to 97% by weight of vaseline and 0.1 to 5% by weight of polyoxyethylene oleyl-ether phosphate in addition to the medicinal fermentation extract of the present invention. 1 to 10% by weight of polyhydric alcohols (propylene glycol, glycerin, etc.) and 0.05 to 2% by weight of a surfactant (polyethylene oleyl ether, polyoxyethylene hardened castor oil, etc.). The nutritional lotion and nutritional cream may contain 5 to 20% by weight of oils (squalane, petrolatum, octyldodecanol, etc.) and 3 to 15% by weight of wax components (cetanol, stearyl alcohol, %, And the essence can be prepared by adding 5 to 30% by weight of polyhydric alcohols such as glycerin, propylene glycol and the like in addition to the fermented extract of the present invention. The massage cream is prepared by adding 30 to 70% by weight of an oil such as liquid paraffin, petrolatum, isononylisononanoate and the like in addition to the medicinal fermented extract of the present invention. In addition to the fermented extract of the present invention, the pack contains polyvinyl alcohol A peel off pack or a general emulsifiable cosmetic composition containing a weight% of a pharmacologically active ingredient of the present invention is characterized in that the pharmaceutical composition of the present invention contains a 5 to 30 wt% pigment such as kaolin, talc, zinc oxide, titanium dioxide, ) Pack.

In addition, the cosmetic composition for relieving skin troubles of the present invention may contain, in addition to the above-described ingredients, the components listed above in addition to the components of ordinary skin cosmetics such as oil, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent, dye, And the like can be appropriately used in combination as needed.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.

[Example]

Example 1

There are many kinds of mushrooms in the market, such as gimgwa, ginseng, mushroom, mushroom, green tea, mushroom, mushroom, mushroom, chrysanthemum, 100 g of the crushed product obtained by purchasing the crushed ice, the mold and the hoiocer were placed in 3 L of purified water or 80% methanol, respectively, and the mixture was boiled for 3 hours in a reflux condenser equipped with a condenser. After filtration through a 100-mesh filter cloth, the residue was extracted one more time by the same method. Each of the extracts was combined and filtered through a filter paper of Whatman No. 2 at room temperature to remove insoluble matters. The extract was concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser, followed by lyophilization, ≪ / RTI > Table 1 shows the dry weight of the extract per 100 g of medicinal product according to the extraction solvent.

Medicinal 80% methanol Purified water Gold silver 7.6 g 6.9 g Sasayam 8.0 g 7.2 g Sophora 6.7 g 6.1 g Skirt mushroom 8.6 g 7.8 g green tea 6.1 g 5.3 g Situation mushroom 9.1 g 8.2 g Scissors 5.7 g 4.9 g Mocker 6.4 g 5.7 g goldthread 7.3 g 6.5 g Changwon 6.8 g 6.0 g magnolia 5.7 g 5.1 g Moon style 6.1 g 5.3 g Bleacher 7.5 g 6.7 g Mutual debt 5.9 g 5.2 g Saury 8.1 g 7.4 g Embarkation 6.5 g 5.7 g Cherry 5.9 g 5.0 g Yugunpi 8.5 g 7.7 g Shipment 6.7 g 5.9 g Ji 6.1 g 5.4 g Car electronics 6.3 g 5.6 g Window 6.5 g 5.8 g Mold 7.2 g 6.5 g Hoi Cho 3.2 g 2.4 g

Examples 2 to 25

Each of the herbal extracts obtained using 80% methanol was added to 5% by weight of YM medium as a basic medium for Saccharomyces cerevisiae, which was a yeast, and fermented.

Specifically, Saccharomyces cerevisiae was inoculated into a yeast basic medium (YM medium) containing 5 wt% of each herbal extract at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, Lt; 0 > C, and aeration amount of 0.05-0.5 VVM. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the yeast organism, and then concentrated under reduced pressure at 60 DEG C in a distilling apparatus equipped with a cooling condenser, followed by freeze-drying to obtain a final fermented yeast fermentation product. The dry weight of the dried fermented product of the medicinal yeast is shown in Table 2 below.

Medicinal Dry weight (g) of fermentation broth Example 2 (silver gold) 6.10 Example 3 (Samam) 6.16 Example 4 (Rhizome) 6.20 Example 5 (Skirt mushroom) 6.05 Example 6 (green tea) 5.89 Example 7 (Situation mushroom) 6.06 Example 8 (Zero) 6.13 Example 9 (poor quality) 6.06 Example 10 (Rhizome) 6.08 Example 11 (Puerariae) 6.16 Example 12 (Magnolia) 6.08 Example 13 (Moon type) 5.91 Example 14 (Baekhwa private reeds) 6.14 Example 15 (Commodity) 5.93 Example 16 (Saururus chinensis) 5.97 Example 17 (Selling) 6.09 Example 18 (cherry) 5.96 Example 19 (yugunpyu) 6.12 Example 20 (Reduction) 6.05 Example 21 (Zymo) 5.99 Example 22 (secondary electron) 5.98 Example 23 (Changer) 6.02 Example 24 (mold) 5.99 Example 25 (Hoicho) 6.08

Examples 26 to 49

Each of the herbal extracts obtained by using 80% methanol was added to 5% by weight of MRS medium as a basic medium for Lactobacillus acidophilus, and fermented.

Specifically, Lactobacillus acidophilus was inoculated into a lactic acid bacterial basic medium (MRS medium) containing 5 wt% of each of the herbal extracts at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, and then pH 6.5 to 7.5 and temperature 35 to 38 , And a ventilation amount of 1 VVM was maintained for 48 hours. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the lactic acid bacteria, followed by concentration under reduced pressure at 60 DEG C in a distilling apparatus equipped with a cooling condenser, followed by lyophilization to obtain a final fermented product of lactic acid bacteria. The dry weight of the fermented product of the medicinal lactic acid bacteria is shown in Table 3 below.

Medicinal Dry weight (g) of fermentation broth Example 26 (Gold Silver) 5.81 Example 27 (crude sap) 6.11 Example 28 (Rhizome) 6.24 Example 29 (skimmed mushroom) 6.11 Example 30 (green tea) 5.93 Example 31 (Shiitake mushroom) 6.12 Example 32 (potato) 6.19 Example 33 (poor quality) 5.95 Example 34 (Rhodiola) 5.96 Example 35 (Puerariae) 5.93 Example 36 (Magnolia) 6.13 Example 37 (Moon Type) 5.86 Example 38 (Baekhwa-ri) 5.91 Example 39 (Commitment) 5.95 Example 40 (Saururus chinensis) 5.93 Example 41 (Selling) 6.02 Example 42 (cherry) 6.11 Example 43 (yugunpyu) 5.96 Example 44 (Red) 6.02 Example 45 (Zymo) 6.07 Example 46 (secondary electron) 6.03 Example 47 (Changer) 6.01 Example 48 (mold) 6.17 Example 49 (Hoi-Cho) 6.13

Examples 50 to 73

Each of the herbal extracts obtained by using 80% methanol was added to the BL medium as a basic medium for bifidobacter bifidobacterium longum at 5 wt% and fermented.

Specifically, Bifidobacterium longum was inoculated into a lactic acid bacterial basic medium (BL medium) containing 5% by weight of each of the herbal extracts at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, and then pH 6.5-7.5, temperature 35-38 ℃, and anaerobic culture conditions. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and bifidus microbial cells. The microbial precipitate and bifidus microbial cells were removed by a distillation apparatus equipped with a cooling condenser, followed by concentration under reduced pressure at 60 DEG C and freeze-drying to obtain a final fermented bifidus microorganism bacterium. The dry weight of the dried fermented product of the medicinal bifidus bacterium is shown in Table 4 below.

Medicinal Dry weight (g) of fermentation broth Example 50 (silver chelate) 6.17 Example 51 (crude sap) 6.05 Example 52 (Persimmon) 6.08 Example 53 (Skirt mushroom) 5.82 Example 54 (green tea) 5.99 Example 55 (Shiitake mushroom) 6.23 Example 56 (Phosphorus) 5.93 Example 57 (poor quality) 6.14 Example 58 (Rhizome) 6.17 Example 59 (Pueraria) 6.03 Example 60 (Magnolia) 6.01 Example 61 (moon type) 6.05 Example 62 (Baihua private use) 6.13 Example 63 (Commodity) 6.18 Example 64 (Saururus chinensis) 5.89 Example 65 (Selling) 6.12 Example 66 (cherry) 6.17 Example 67 (yugunpyu) 5.89 Example 68 (Red) 5.94 Example 69 (Zymo) 6.11 Example 70 (secondary electron) 6.19 Example 71 (Changer) 5.98 Example 72 (mold) 6.16 Example 73 (Hoi-Cho) 6.06

Examples 74 to 97

The medicinal materials used in Table 1 were sectioned and added with 5% by weight of fungi for solid fermentation.

Specifically, 200 g of each of the medicinal materials was used as a slice, Paecilomyces japonica was inoculated at 5% by weight under constant humidity, and the mixture was fermented for 60 days at a temperature of 18 to 25 캜 and a humidity of 60 to 70% g was added to 3 L of 80% methanol, and the mixture was boiled for 3 hours in a reflux condenser equipped with a condenser. After filtration with a 100 mesh filter cloth, the residue was extracted one more time by the same method. Each of the extracts was combined and filtered through a filter paper of Whatman No. 2 at room temperature to remove insoluble matter. The extract was concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser and then lyophilized to obtain a final fermented product of the medicinal fungus. The dry weight of the dried fermented product of the medicinal fungus is shown in Table 5 below.

Medicinal Dry weight (g) of fermentation broth Example 74 (silver gold) 6.92 Example 75 (crude sap) 7.12 Example 76 (Rhizome) 6.31 Example 77 (skimmed mushroom) 7.88 Example 78 (green tea) 5.57 Example 79 (Shiitake mushroom) 8.05 Example 80 (Prune) 5.17 Example 81 (poor quality) 5.83 Example 82 (Rhizome) 6.52 Example 83 (Pueraria) 6.09 Example 84 (Magnolia) 5.21 Example 85 (Moon type) 5.48 Example 86 (Baihua private use) 6.56 Example 87 (Commodity) 5.28 Example 88 (Saururus chinensis) 7.22 Example 89 (Esterification) 5.76 Example 90 (cherry) 5.28 Example 91 (yugunpyu) 7.63 Example 92 (Red) 6.05 Example 93 (Zymo) 5.42 Example 94 (secondary electron) 5.53 Example 95 (Changer) 5.76 Example 96 (mold) 6.43 Example 97 (Hoi-Cho) 3.11

Experimental Example 1. Inhibitory effect on PGE2 production

To confirm the anti-inflammatory effect of each of the medicinal materials themselves and the medicinal fermentation extracts of Examples 2 to 97, the effect of inhibiting PEG2 formation was examined. (Jeffrey, Kh, Anglea, SW, Zoe, S. and Rhia CM Intracellular Measurement of Prostaglandin E2: Effect of Anti-Inflammatory Drugs on Cyclooxygenase Activity and Prostanoid Expression, Analytical Biochemistry, 1999, 271, 18-28).

Fibroblasts isolated from human epidermal tissue were subcultured several times, and then put into a 96-well plate in an amount of 1 × 10 ^5, and cultured for 24 hours. FBS-free medium and cultured for 2 hours. The respective medicinal materials themselves and the medicinal fermentation extracts of Examples 2 to 97 were treated at the concentrations shown in the following Table 6, whereby indomethacin was also compared After incubation for 1 hour, calcium ionophore A23187 and arachidonic acid were treated at a concentration of 50 mM for stimulation for 5 minutes at 37 ° C. Cells were treated with dodecyltrimethlammonium bromide for 10 minutes at a final concentration of 0.25%, and the amount of PGE2 was quantitated using an enzyme-linked immunosorbent assay (ELISA) by taking an appropriate amount of the supernatant. The prostaglandin inhibitory effect of the herbal medicine fermentation broth was determined, and the results are shown in Tables 6 to 8 below.

sample PEG increase (pg / well) % Inhibition Control (negative control) - - Arachidonic acid (positive control) 219 - Indomethacin (positive control) 14 93.6 YM badge 195 11.0 MRS badge 183 16.4 BL medium 190 13.2 Gold silver Medicinal self 155 29.2 The yeast fermented extract of Example 2 120 45.2 The fermented extract of lactic acid bacteria of Example 26 116 47.0 The bifidobacterial fermentation extract of Example 50 125 42.9 The fungal fermentation extract of Example 74 119 45.7 Sasayam Medicinal self 136 37.9 The fermented yeast extract of Example 3 105 52.1 The fermented extract of lactic acid bacteria of Example 27 102 53.4 The bifidobacterial fermentation extract of Example 51 110 49.8 The fungal fermentation extract of Example 75 95 56.6 Sophora Medicinal self 139 36.5 The fermented yeast extract of Example 4 112 48.9 The fermented extract of lactic acid bacteria of Example 28 120 45.2 The bifidobacterial fermentation extract of Example 52 108 50.7 The fungal fermentation extract of Example 76 111 49.3 Skirt mushroom Medicinal self 140 36.1 The fermented yeast extract of Example 5 125 42.9 The fermented extract of lactic acid bacteria of Example 29 127 42.0 The bifidobacterial fermentation extract of Example 53 110 49.8 The fungal fermentation extract of Example 77 116 47.0 green tea Medicinal self 141 35.6 The yeast fermented extract of Example 6 127 42.0 The fermented extract of lactic acid bacteria of Example 30 119 45.7 The bifidobacterial fermentation extract of Example 54 115 47.5 The fungal fermentation extract of Example 78 112 48.9 Situation mushroom Medicinal self 137 37.4 The yeast fermented extract of Example 7 102 53.4 The fermented extract of lactic acid bacteria of Example 31 99 54.8 The bifidobacterial fermentation extract of Example 55 104 52.5 The fungal fermentation extract of Example 79 107 51.1 Scissors Medicinal self 151 31.1 The yeast fermented extract of Example 8 130 40.6 The fermented extract of lactic acid bacteria of Example 32 122 44.3 The bifidobacterial fermentation extract of Example 56 121 44.7 The fungal fermentation extract of Example 80 118 46.1 Low thread Medicinal self 142 35.2 Example 9 Yeast Fermentation Extract 127 42.0 The fermented extract of lactic acid bacteria of Example 33 112 48.9 The bifidobacterial fermentation extract of Example 57 121 44.7 Example 81 Mold fungus extract 120 45.2

sample PEG increase (pg / well) % Inhibition goldthread Medicinal self 145 33.8 The yeast fermented extract of Example 10 127 42.0 The fermented extract of lactic acid bacteria of Example 34 117 46.6 The bifidobacterial fermentation extract of Example 58 120 45.2 The fungal fermentation extract of Example 82 111 49.3 Changwon Medicinal self 140 36.1 The fermented yeast extract of Example 11 122 44.3 The fermented extract of lactic acid bacteria of Example 35 115 47.5 The bifidobacterial fermentation extract of Example 59 109 50.2 The fungal fermentation extract of Example 83 117 46.6 magnolia Medicinal self 150 31.5 The fermented yeast extract of Example 12 127 42.0 The fermented extract of lactic acid bacteria of Example 36 126 42.5 The bifidobacterial fermentation extract of Example 60 114 47.9 The fungal fermentation extract of Example 84 119 45.7 Moon style Medicinal self 153 30.1 The fermented yeast extract of Example 13 127 42.0 The fermented extract of lactic acid bacteria of Example 37 114 47.9 The bifidobacterial fermentation extract of Example 61 134 38.8 The fungal fermentation extract of Example 85 126 42.5 Bleacher Medicinal self 139 36.5 The fermented yeast extract of Example 14 110 49.8 The fermented extract of lactic acid bacteria of Example 38 109 50.2 The bifidobacterial fermentation extract of Example 62 104 52.5 The fungal fermentation extract of Example 86 113 48.4 Mutual debt Medicinal self 146 33.3 The yeast fermented extract of Example 15 116 47.0 The fermented extract of lactic acid bacteria of Example 39 122 44.3 The bifidobacterial fermentation extract of Example 63 124 43.4 The fungal fermentation extract of Example 87 116 47.0 Saury Medicinal self 139 36.5 The yeast fermentation extract of Example 16 115 47.5 The fermented extract of lactic acid bacteria of Example 40 113 48.4 The bifidobacterial fermentation extract of Example 64 126 42.5 The fungal fermentation extract of Example 88 111 49.3 Embarkation Medicinal self 143 34.7 The yeast fermented extract of Example 17 125 42.9 The fermented extract of lactic acid bacteria of Example 41 127 42.0 The bifidobacterial fermentation extract of Example 65 117 46.6 The fungal fermentation extract of Example 89 121 44.7 Cherry Medicinal self 147 32.9 The yeast fermented extract of Example 18 123 43.8 The fermented extract of lactic acid bacteria of Example 42 110 49.8 The bifidobacterial fermentation extract of Example 66 119 45.7 The fungal fermentation extract of Example 90 122 44.3

sample PEG increase (pg / well) % Inhibition Yugunpi Medicinal self 135 38.4 Example 19 Yeast Fermentation Extract 104 52.5 The fermented extract of lactic acid bacteria of Example 43 109 50.2 The bifidobacterial fermentation extract of Example 67 97 55.7 The fungal fermentation extract of Example 91 103 53.0 Shipment Medicinal self 150 31.5 The yeast fermented extract of Example 20 114 47.9 The lactic acid bacteria fermented extract of Example 44 120 45.2 The bifidobacterial fermentation extract of Example 68 122 44.3 The fungal fermentation extract of Example 92 125 42.9 Ji Medicinal self 147 32.9 The fermented yeast extract of Example 21 120 45.2 The fermented extract of lactic acid bacteria of Example 45 129 41.1 The bifidobacterial fermentation extract of Example 69 114 47.9 The fungal fermentation extract of Example 93 117 46.6 Car electronics Medicinal self 147 32.9 The yeast fermented extract of Example 22 117 46.6 The fermented extract of lactic acid bacteria of Example 46 120 45.2 The bifidobacterial fermentation extract of Example 70 125 42.9 The fungal fermentation extract of Example 94 119 45.7 Window Medicinal self 146 33.3 The fermented yeast extract of Example 23 118 46.1 The fermented extract of lactic acid bacteria of Example 47 119 45.7 The bifidobacterial fermentation extract of Example 71 126 42.5 The fungal fermentation extract of Example 95 120 45.2 Mold Medicinal self 151 31.1 The yeast fermented extract of Example 24 117 46.6 The fermented extract of lactic acid bacteria of Example 48 120 45.2 The bifidobacterial fermentation extract of Example 72 122 44.3 The fungal fermentation extract of Example 96 125 42.9 Hoi Cho Medicinal self 141 35.6 The fermented yeast extract of Example 25 107 51.1 The fermented extract of lactic acid bacteria of Example 49 112 48.9 The bifidobacterial fermentation extract of Example 73 117 46.6 The fungal fermentation extract of Example 97 113 48.4

As shown in Tables 6 to 8, the medicinal plant fermentation extracts obtained according to Examples 2 to 97 exhibited a higher inhibition rate of prostaglandin (PGE2) than the respective medicinal materials or the basic medium, thereby inhibiting the induction or inhibition of COX-2 enzyme It was found that the anti-inflammatory effect was excellent.

The following are examples of preparing cosmetic compositions using the medicinal fermented extract according to the present invention, which is excellent in anti-inflammatory and anti-irritating effect and excellent in skin troubles-relieving function and safe to the skin.

Example 98 and Comparative Example 1

The ointment extracts of Example 74 were used to prepare ointment for external use on the skin as shown in Table 9 below.

division Example 74 Comparative Example 1 Fermented extract 10 - Diethyl sebacate 8 8 Nonsense 5 5 Polyoxyethylene oleyl ether phosphate 6 6 Sodium benzoate Suitable amount Suitable amount vaseline To 100 To 100

Example 99 and Comparative Example 2

The cream of Example 74 was prepared according to the prescription of Table 10 using the fermented extract of Gingko fungus.

division Example 74 Comparative Example 2 Fermented extract 1.0 - Stearic acid 15.0 15.0 Cetanol 1.0 1.0 Potassium hydroxide 0.7 0.7 glycerin 5.0 5.0 Propylene glycol 3.0 3.0 antiseptic Suitable amount Suitable amount incense Suitable amount Suitable amount Purified water To 100 To 100

Example 100 and Comparative Example 3

Using the fermented extract of Gingko fungus of Example 74, the softening longevity was prepared according to the prescription of Table 11 below.

division Example 100 Comparative Example 3 Fermented extract 0.2 - ethanol 10.0 10.0 Polyoxyethylene sorbitan polylauric acid 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 glycerin 5.0 5.0 1,3-butylene glycol 6.0 6.0 incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water To 100 To 100

Example 101 and Comparative Example 4

Nutritive lotion was prepared according to the formulation shown in Table 12 below using the fermented gruff mold extract of Example 74.

division Example 101 Comparative Example 4 Fermented extract 0.1 - Vaseline 2.0 2.0 Sesquioleic acid sorbitan 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 Methyl paraoxybenzoate Suitable amount Suitable amount Propylene glycol 5.0 5.0 ethanol 3.2 3.2 Carboxyvinyl polymer 18.0 18.0 Potassium hydroxide 0.1 0.1 Pigment Suitable amount Suitable amount incense Suitable amount Suitable amount Purified water To 100 To 100

Example 102 and Comparative Example 5

Using the fermented gruff mold extract of Example 74, a pack was prepared according to the prescription shown in Table 13 below.

division Example 102 Comparative Example 5 Fermented extract 0.5 - glycerin 5.0 5.0 Propylene glycol 4.0 4.0 Polyvinyl alcohol 15.0 15.0 ethanol 8.0 8.0 Polyoxyethylene oleylethyl 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water To 100 To 100

Example 103 and Comparative Example 6

The essence was prepared by using the ferulicidal fermented extract of Example 74 as described in Table 14 below.

division Example 103 Comparative Example 6 Fermented extract 5.0 - Propylene glycol 10.0 10.0 glycerin 10.0 10.0 Sodium hyruronate aqueous solution (1%) 5.0 5.0 ethanol 5.0 5.0 Polyoxyethylene hardened castor oil 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 incense Suitable amount Suitable amount Purified water To 100 To 100

Experimental Example 2. Anti-inflammatory effect

To investigate the anti-inflammatory effect of the cosmetic preparation prepared in Example 98, a method using arachidonic acid (A. Crummey, GP Harper, EA Boyle and FR Mangan, Inhibitory of arachidonic acid-induced ear edema as a model for assessing Topical anti-inflammatory compound (Agents and Actions, 1987, 20: 69-76).

First, 35 mice (hairless mice) were divided into 7 animals. Both ears were cleaned with ethanol before application of the sample, and then measured using a micrometer. The cosmetic compositions of Example 46 and Comparative Example 1, indomethacin indomethacin) 1.0% and a control group which treated only arachidonic acid, and the average value of the ear thickness of the mouse was measured. 20 μl of the sample was continuously applied to the sample group for treating the cosmetic of Example 98 and the cosmetic of Comparative Example 1 and the comparative group for treating 1.0% of indomethacin, and 20 μl of ethanol was continuously applied for 4 days once a day to the control group. After the last application and curing for 1 hour, ethanol was applied to the left ear and arachidonic acid was applied to the right ear at 2 mg / ear. After 1 hour of application, the degree of edema of the ear was repeated three times for both ears using a micrometer Respectively. The left ear of the treated group was used as a control group to examine the degree of inflammation induced by the sample itself. The anti-inflammatory effect was evaluated based on the control group treated with arachidonic acid alone. The results are shown in Table 15 below.

The inhibition rate was measured according to the following equation (1).

[Equation 1]

Inhibition rate (%) =

A is the average thickness change of the control ear (the thickness of the arachidonic acid treatment pad - the thickness of the untreated arachidonic acid ear), B is the average thickness change of the sample application group ear (the thickness of the treated ear, to be.

division Concentration (%) Glume (㎛) % Inhibition Before sample treatment After sample treatment Sample fungus Example 98 - 355 541 22.5 Comparison group 1 Comparative Example 1 - 350 559 12.9 Comparison group 2 Indomethacin 1.0 357 497 41.7 Control group Arachidonic acid 2 mg / ear 333 573 -

As shown in Table 14, the cosmetic composition of Example 98 according to the present invention exhibited an inflammation inhibition rate of 25.8%, which is lower than that of indomethacin, which is an excellent anti-inflammatory substance as a pharmaceutical ingredient, As shown in FIG.

Claims (4)

A cosmetic composition for inhibiting prostaglandin E2 comprising a fermented extract of Angelica keiskei koidz. As an active ingredient. The method according to claim 1,
The fermented extract may be selected from the group consisting of Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids , Saccharomyces boulardii , Lactobacillus acidophilus, Lactobacillus acidophilus , Lactobacillus casei , Lactobacillus bulgaricus , Lactobacillus rhamnosus , Lactobacillus plantarum , Lactobacillus reuteri , Lactobacillus confusus, Lactobacillus spp . , Lactobacillus spp . , Lactobacillus spp. Lactobacillus fermentum , Lactobacillus brevis , Bifidobacterium breve , Bifidobacterium bifidum , Bifidobacterium longum, Bifidobacterium longum, Bifidobacterium longum, Long sword) and Bifidobacterium infantis (Bifidobacterium infantis (Prostaglandin E2) inhibitory composition comprising a fermented extract of herbal medicines obtained by adding and cultivating persimmon when cultivating at least one bacterium selected from the group consisting of herbaceous plant extracts and herbaceous plants. The method according to claim 1,
The fermented extracts may contain at least one fungi selected from the group consisting of Inonnotus-obliquus, Cordyceps sinensis, Paecilomyces japonica, phellinus linteus and Ganoderma lucidum, And the fermented extract obtained by solid fermentation is contained as an active ingredient. The cosmetic composition for inhibiting prostaglandin E2 according to claim 1, The method according to claim 1,
Wherein the fermented extract is contained in the cosmetic composition in an amount of 0.0001 to 10% by weight.