KR101441953B1 - Method and System for Measurement of Analytes in Samples - Google Patents

Abstract

The present invention relates to a reaction device comprising (a) a reaction device comprising (i) a color reaction means for applying a sample to induce a color reaction and (ii) a color-correction verification means having a pre- Applying a sample to the color reaction means to induce a color reaction in the color reaction means; (b) a reference-result synchronized image comprising a first image as an image of the color correction verification means in the device and a second image as an image of a result of the color reaction in a digital imaging device, At the same time as one image; Wherein the first image and the second image are obtained as one image simultaneously under the same light environment, (c) comparing the first image and the second image in the contrast-result synchronous image, Calibrating color; And (d) obtaining data on the presence or concentration of the analyte in the sample from the color of the corrected second image. According to the present invention, data on the presence or concentration of an analyte in a sample can be obtained easily and accurately.

Description

TECHNICAL FIELD The present invention relates to a method and a system for measuring an analyte in a sample,

The present invention relates to a measurement method and a measurement system for an analyte in a sample using a digital imaging device.

Methods for analyzing materials include gas chromatography, mass spectrometry, high-performance liquid chromatography, thin-layer chromatography, electrophoresis, ), Electrochemistry, Infrared Spectroscopy, Raman Spectroscopy, X-ray Flouescene, Ultraviolet / Visible Spectroscopy, and the like. Methods are widely used in industries such as natural science and engineering research, and medical, pharmaceutical, and environmental applications.

Most of the above-described analysis methods require expensive equipment, are difficult to use, and are difficult to be applied in a miniaturized or portable manner. On the other hand, the electrochemical method has already been commercialized because it can be easily measured and transported at low cost and is already applied to blood glucose test. However, since the electrochemical method is applied only to a reaction capable of generating electrons, there is a disadvantage that its utilization is limited.

On the other hand, the optical detection method of measuring the radiation energy that is transmitted, reflected, or refracted to an object as a function of wavelength is easy and accurate in use as well as an electrochemical measurement method, Can be used. Analytical methods that use these principles are applied in a wide variety of fields such as chemistry, physics, biochemistry, immunology, enzymology, molecular biology, and food science.

For example, Korean Patent Registration No. 10-0384795 discloses an analytical technique for measuring a color reaction using a kinetic type using a spectrophotometer. In Korean Patent Registration No. 10-0251999, a strip- Discloses a method of reading analysis results using electromagnetic radiation such as visible light. Korean Patent Publication No. 10-1092351, US Patent No. US7538336, US Patent No. 7803322, US Patent No. 7267799 and US Patent Publication No. US20030049849A1 disclose a method of measuring the reaction result in the form of strip in reflectance. The above analysis method has the disadvantage that the versatility is deteriorated because it is necessary to insert the analysis result into a device in a specific type of device in order to read the analysis result on the light absorption or reflectance. US Pat. No. 6,586,257 describes a system for scanning and analyzing color, fluorescence, and chemiluminescence appearing in multiwells, but it is difficult to use because it requires expensive equipment and expert knowledge.

The above analytical methods and systems use devices comprising at least one or more light sources and can be used to generate color or label deposition reactions in solid or liquid reactions in specific devices in the form of strips or tubes And the reaction device is inserted into the apparatus to measure the state in which the external light is blocked. This analysis method has a disadvantage that it is difficult to apply when a device of a certain type is used and an analyzing device measures the device, so that it is difficult to apply when the device type is different. Therefore, it is inconvenient to purchase each of the analyzers suitable for each test for the various types of tests, and to perform the measurement results according to the respective devices.

The present inventors have made efforts to develop a method for easily measuring an analyte in a sample using a device including a digital imaging device. As a result, the present inventors obtained a first image of the color correcting control means and a second image as a result of the color reaction in the reaction device simultaneously using the digital imaging device, and using the color correcting verification means, The present invention has been accomplished by identifying a method for correcting the color of an image and easily analyzing the presence or concentration of an analyte in the sample from the color of the second image.

Accordingly, it is an object of the present invention to provide a method for measuring an analyte in a sample.

It is another object of the present invention to provide a system for measuring an analyte in a sample.

According to one aspect of the present invention, the present invention provides a method of measuring an analyte in a sample comprising the steps of:

(a) a reaction device comprising (i) a color reaction means for applying a sample to induce a color reaction, and (ii) a control means for color correction having a pre- Thereby inducing a color reaction to occur in the color reaction means;

(b) a reference-result synchronized image comprising a first image as an image of the color correction verification means in the device and a second image as an image of a result of the color reaction in a digital imaging device, At the same time as one image; The first image and the second image are obtained as one image at the same time under the same light environment,

(c) comparing the first image and the second image in the contrast-result synchronous image to calibrate the color of the second image; And

(d) obtaining data on the presence or concentration of the analyte in the sample from the color of the corrected second image.

The present inventors have sought to develop a method for easily measuring an analyte in a sample using a digital imaging device. As a result, the present inventors obtained a first image of the color correcting control means and a second image as a result of the color reaction in the reaction device simultaneously using the digital imaging device, and using the color correcting verification means, It was found that the color of the image can be corrected and the presence or concentration of the analyte in the sample can be easily analyzed from the color of the second image.

According to the present invention, it is possible to simultaneously obtain the contrast means image and the result image under the same condition by simultaneously capturing the image of the color correcting means and the result of the color reaction, and simply correct the color of the resultant image from the contrast means image, A simple and accurate analysis result on the analyte in the sample can be obtained.

According to a preferred embodiment of the present invention, the sample used in the present invention may be a blood, a plasma, a serum, a urine, a lymph fluid, a bone marrow fluid, a saliva, a milk, an ocular fluid, a semen, a brain extract, a spinal fluid, A blood serum, a serum, a urine, a lymphatic fluid, a bone marrow fluid, a saliva, a milk, a spinal fluid, a cell tissue fluid, a buffer solution, tap water or wastewater, More preferably blood, plasma, serum or urine.

The analyte in the present invention may be a protein, a gene, a lipid, a carbohydrate, an amino acid, a nucleic acid, a monosaccharide, a vitamin, a hormone, a metabolite, a drug, an antibiotic, an agricultural chemical, an insecticide and an environmental pollutant. The analyte is measured through a color reaction such as coloration or discoloration occurring through a single or multi-step process, and a color reaction by labeling such as coloring matter, metal nanoparticles, and colored polymer.

The samples of the present invention may be present singly or in the form of a mixture, and may exist in solid or liquid form.

The measuring method of the present invention can be used in various fields such as diagnosis and prediction of disease, health care, paternity confirmation, constitution confirmation, fermentation engineering, biotechnology, food safety inspection, environmental analysis, cosmetic analysis and compound analysis.

In the present invention, a reaction device for applying a sample to induce a color reaction may employ various devices known in the art.

The reaction device used in the present invention includes a strip containing a solid material such as a nitrocellulose membrane, cellulosic paper, glass fiber, PVDF membrane, glass, plastic, etc., which can be immobilized to detect an analyte from a sample, Lt; / RTI > The reaction device of the present invention can be any shape such as a rectangle, a square, a right triangle, an equilateral triangle, a multi-circle, and a circle.

The reaction device in the present invention includes a control means for color correction.

In the present invention, the control means for color correction can be provided in two broad ways:

In the first method, the color correcting means may be provided separately from the color correcting means in the color correcting table printed with the color correcting means.

In the second method, the color correcting control means can be provided integrally with the color reaction means. That is, the color correcting control means may be provided on the same surface adjacent to and on the same color support means as the color reaction means.

According to the first method, an image of a color correction control means in the form of a colorimetric table provided separately from an image of a result of a color reaction in the color reaction means is simultaneously obtained as an image. Products such as urine test, pH test and nitrate test, which are currently being sold, are provided with a strip-shaped reaction device and printing a colorimetric chart on packaging paper or paper. Therefore, the color of the reaction part of the reaction device is visually checked against the colorimetric table to recognize the degree of the reaction. By using the above method, color matching control means can be separately provided in a packaging container or paper according to a conventional method of visually inspecting, and the degree of color change of a reaction device can be inspected using a digital imaging device.

However, in the case of the first method, it is inconvenient to individually set the checking means for color correction for each reaction or inspection. In addition, since it is very difficult to actually implement the image color and the print color acquired by the naked eye or the digital imaging device in the same manner, a considerable effort is required in order to apply it to various tests. In particular, when performing various kinds of inspections in a single device, the number of checking means for color correcting becomes several tens, which is disadvantage in that it is not easy to apply such a method. For example, Bayer's MultiStix ^TM For 10SG, 10 kinds of urine test strips for glucose, ketone, bilirubin, etc. are provided with color charts containing more than 50 colors. In order to inspect them using digital imaging device, It is not easy to apply because it needs to print. In addition, since the color correcting means for color correction is too large and complicated when photographing with a digital imaging device, it is difficult to precisely locate the image and it is difficult to cope with color or shape distortion of the image. In addition, when the contrasting means for color correction is provided separately from the device, it is necessary to obtain images by horizontally arranging the device and the control means horizontally. Therefore, it is very inconvenient to apply vertical state measurement such as a test tube reaction .

Therefore, according to a preferred embodiment of the present invention, the color correcting control means in the present invention is provided on the same surface adjacent to and on one support with the color reaction means in accordance with the second scheme. In this case, the problems of the first method described above can be overcome.

In the present invention, since the color correcting control means should be contained in the reaction device, it is preferable that 10 colors or less (for example, white, red, blue, green, etc.) or 5 or fewer colors are used, It is less than branches. When the reaction device includes the color correcting contrasting means, it is possible to apply the same to various tests.

When the color correction contrasting means is composed of a plurality of colors, preferably this means can be used as a coding system. As used herein, the term " coding system " refers to an optical instrument-read representation of data associated with an object to which the coding system is attached. In other words, the " coding system " in the present invention means an indicator of data such as a kind of inspection related to a reaction device to which a coding system is attached, an image information processing method and a test result display method. The device of the present invention may have a coding system separately as described later, and in the case where the color correction verification means is composed of a plurality of colors, the color correction verification means may be used as a coding system.

In the present invention, the color correction verification means is used to reduce the deviation of the image information with respect to the light source of the digital imaging apparatus. For example, when white light having high illuminance and white light having low illuminance are used as a light source, a difference occurs in image data. This difference can be corrected by color matching means to perform more accurate analysis.

According to a preferred embodiment of the present invention, the digital imaging device in the present invention comprises a camera module. An image sensor, which is an image sensing device of an image sensing device, includes a device that receives light and converts it into an electric signal. The image sensor includes a charge coupled device (CCD) type image sensor or a complementary metal-oxide semiconductor (CMOS) ) Type image sensor. The CCD type image sensor and the CMOS type image sensor commonly have a light receiving portion that receives light and converts it into an electric signal. The CCD type image sensor transmits the electric signal through the CCD, converts it to a voltage at the last stage, The image sensor converts the signal to a voltage at each pixel and outputs it to the outside. The digital imaging device converts the light passing through the lens into an electric signal through the image sensor, then converts the image into analog data, and then processes the digital data through the AD converter. Although the image digital data can be analyzed by a separate device such as a personal computer, it is preferable that the image processing is performed in the same electronic device. The digital image pickup apparatus of the present invention preferably includes a microcontroller chipset to compress image digital data changed by the AD converter and can perform color correction, contour enhancement, noise control, gamma value correction, gradation (gradation) , And the modified digital data is stored in a storage device inside or outside the digital imaging device.

The digital imaging device of the present invention is a digital imaging device that includes a digital imaging device and is capable of image correction through a microprocessor and can store results, and more preferably a portable multimedia player (PMP), a personal digital assistant ), A smart phone, a digital camera, a laptop or a tablet PC, and more preferably a smart phone.

The digital imaging device in the present invention preferably includes analysis means capable of analyzing the presence or concentration of the analyte from the image data within the device. By using the analysis means, it is possible to analyze the presence or concentration of the analyte by comparing the intensity or the change of hue from the image data of the color reaction result generated on the reaction device.

According to a preferred embodiment of the present invention, the reaction device of the present invention can attach a coding system such as a barcode to the inside or the outside of the reaction device. When the digital imaging device recognizes it, the type of inspection for the sample, And a method of displaying a result can be called up (see FIG. 3-4). Preferably, when the coding system is recognized by using the digital imaging device, analysis means in which the type and type of examination are stored in advance is displayed, and parentheses, squares or circles are displayed on the screen of the digital imaging device, (See Figs. 3-4). Also, the color correcting control means or color reaction result is displayed on the screen in the form of parentheses, and the focus is manually or automatically obtained. For example, when focusing manually, a shutter can be used like a digital camera, and an image can be automatically captured when a certain size and position match.

According to a preferred embodiment of the present invention, a contrast-result synchronous image comprising a first image as an image of the color-correcting control means of the present invention and a second image as an image of the result of the color- Can be obtained at the same time.

According to a preferred embodiment of the present invention, the optical environment for obtaining a synchronous image is under an external light source or an internal light source.

The term " light source " as used herein includes any device that emits light, including natural light, and includes, for example, a light bulb, a light emitting diode (LED), and a laser. The reflector may further include a reflector for concentrating or dispersing illumination emitted from the light source.

The reaction device used in the present invention preferably includes single or plural color correction means for white, red, blue, and green, and can calibrate the reaction result using the same.

According to a preferred embodiment of the present invention, the color of the present invention can be displayed using color spaces such as RGB, HSB, CMYK, CIELab, and CIEXYZ, and the coordinate values on the color space for each color can be switched And the formulas related thereto are already known. For example, the red color represented by RGB (255, 0, 0) is HSB (0, 100, 100), the color is the same as CIELab (53, 80, 67) Green is HSB (120, 100, 100), and is the same color as CIELab (88, -86, 83).

According to a preferred embodiment of the present invention, the color correction verification means having a specific coordinate can be changed according to the external environment (for example, the type and brightness of the light source), and by setting the correction value for that, the change in the external environment can be minimized have. For example, an orange color having HSB coordinates (32, 100, 100) or RGB coordinates (255, 136, 0) is displayed as HSB coordinates (32, 100, 80) or RGB coordinates (204, 109, 0) . When one or more color matching means is included in the device and the image is analyzed in this way, the position of each coordinate can be corrected by applying an algorithm suitable for the device. (2002), Agawal et al., J Pattern < RTI ID = 0.0 > Recognition Research 1 , 42-54 (2006)). In the present invention, the influence on the external environment can be minimized by using a known algorithm or applying a new algorithm.

In the present invention, it is preferable to use an average value of combinations of two or more selected ones of the coordinate values on the color space at a specific position of the image in which the reaction appears. Even more preferably, the above coordinate value is an average value of combinations of three or more.

According to the present invention, the digital imaging device in the present invention can include a communication module. The communication module used in the present invention can use various communication modules known in the art. The data can be transmitted to the same or different digital imaging apparatus by wire or wirelessly using the communication module.

According to a preferred embodiment of the present invention, the digital imaging apparatus according to the present invention further includes transmission means for transmitting the image coming from the reaction device or the analysis result coming from the analysis program to the user via wire or wirelessly. For example, if the analysis result from the analysis program is wirelessly transmitted through the transmission means, a third generation (3G) wideband code division multiple access network (WCDMA), a wireless LAN (Wi-Fi), a WiBro Users can transmit and receive using Evolution network (LTE).

According to another aspect of the present invention there is provided a system for measuring an analyte in a sample comprising:

(a) a reaction device comprising (i) a color reaction means for applying a sample to induce a color reaction to occur, and (ii) a control means for color correction having a pre-determined color;

(b) a digital imaging device; Said digital imaging device comprising a reference-resultant image comprising a first image as an image of said color correction verification means in said reaction device and a second image as an image resulting from a color reaction in said color reaction means, result synchronized image simultaneously in one image under the same light environment;

(c) comparing the first image and the second image in the contrast-result synchronous image to calibrate the color of the second image and determine the presence or absence of the analyte in the sample from the color of the corrected second image Or analyzing means for providing data on concentration.

The system of the present invention relates to an apparatus for implementing the above-described method of measuring analytes in a sample of the present invention, the description of which is common to avoid the excessive complexity of the present specification.

The system of the present invention comprises a reaction device, a digital imaging device and an analysis means. The reaction device (1) comprises any material and shape by which a sample can be applied to cause a color reaction. Preferably, the reaction device (1) comprises a support (11), a color reaction means (12) and a control means for color correction (13).

The support 11 includes any support conventionally used in the art, preferably a polymer (e.g., polystyrene and polypropylene), glass, metal, or paper. The reaction with the sample is induced in the color reaction means 12 on the support to generate a color reaction. Preferably, the color correcting means 13 is located on the same side of the support as the color reaction means 12 from which the color reaction result is derived.

According to a preferred embodiment of the present invention, the color correction verification means included in the reaction device of the present invention is utilized as a coding system. For example, in the case of using the white, red, green, and blue color matching means, 24 (4 x 3 x 2 x 1) types of coding are possible. In this case, preferably, the analyzing means in the present invention includes (i) information such as a kind of inspection for a sample having a correlation with the coding system, an image information processing method and a test result display method, and (ii) And a processor for providing data on the presence or concentration of the analyte in the sample based on the information having a correlation with the system.

For example, if a coding system is recognized using a digital imaging device, the processor may be configured to provide data on the presence or concentration of an analyte in the sample based on information such as the type of examination, the method of processing the image information, And parentheses, squares or circles are displayed on the screen of the digital imaging device to adjust the distance of the reaction device and the digital imaging device (see FIG. 3-4).

The features and advantages of the present invention are summarized as follows:

(I) The present invention provides a method for measuring an analyte in a sample.

(Ii) The present invention can easily and accurately derive data on the presence or concentration of an analyte in a sample.

(Iii) The present invention provides a system for measuring an analyte in a sample.

1 is a schematic view of a reaction device 1 of the present invention. A support 11, a color reaction unit 12, and a color correction control unit 13.
(A) R ", (b) G" in which the RGB values are corrected by using a calibration means for correction, after obtaining an image using a digital imaging device after performing nitrite detection by concentration according to the present invention, And (c) the B "value.
FIG. 3A is a schematic view showing a process of recognizing a color correction verification means included in a reaction device using a digital imaging device as a coding system. FIG.
Fig. 4 is a schematic diagram of a control-color image forming apparatus including a first image as an image of a color-correcting control means 13 in a reaction device and a second image as an image of a result of a color- The result is a schematic diagram showing the process of obtaining a synchronous image.
5 is a schematic diagram showing a process of deriving an analysis result from a contrast-result synchronous image through the analysis means included in the digital imaging device.
DESCRIPTION OF REFERENCE NUMERALS
1: Reaction device 11: Support
12: color reaction means 13: color contrast control means

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Example 1: Image correction of a pH strip

(0, 255, 0), CIELab (88, -86, 83) green, RGB (0, 0, 255), CIELab White contrasting means such as CIELab (32, 79, -108), RGB (255, 255, 255) and CIELab (100, 0, 0) are cut out from the standard color table of Pantone into band shapes, After attaching to the pH-Fix test strip of Macherey-Nagel (Germany) with pad, Samsung Electronics Galaxy S and Samsung Galaxy SII, which are smart phones, are used as digital imaging devices. . The obtained images were averaged over three points for each band using an image analysis program, and the RGB values were obtained. The results are shown in Table 1.

Device Galaxy S Galaxy SII collection One 2 3 4 5 6 color R G B R G B R G B R G B R G B R G B Red
Contrast means 131 14 30 152 25 40 175 34 51 117 15 31 136 40 53 163 53 76 green
Contrast means 10 84 56 21 107 76 25 126 93 One 87 55 13 113 82 22 138 113 blue
Contrast means 4 34 63 12 44 91 20 59 104 2 32 77 15 50 95 25 65 127 White
Contrast means 150 153 136 175 181 163 200 203 186 114 158 141 151 191 177 180 213 208 Pad 1 146 68 44 176 93 68 197 109 82 133 63 37 153 93 67 182 120 95 Pad 2 154 105 43 181 132 63 201 151 76 133 106 17 159 144 52 187 171 76 Pad 3 98 92 64 127 118 89 147 141 110 71 97 60 102 130 92 128 153 123 Pad 4 150 103 38 176 127 51 200 148 64 133 102 9 156 137 40 188 169 66

As can be seen from the above table, the same RGB values as those of the standard color trademark could not be obtained due to differences in illumination or image acquisition process, and color variations of the control means and the pad were varied as shown in Table 2.

division R G B Average Standard Deviation Average Standard Deviation Average Standard Deviation Pad 1 165 24.3 91 22.3 66 22.0 Pad 2 169 25.0 135 26.0 55 22.5 Pad 3 112 27.1 122 24.2 90 24.8 Pad 4 167 25.2 131 26.1 45 21.0

A method of correcting the color of an image can be applied in a wide variety of ways. In this embodiment, the color is corrected using a comparator by a relatively simple calculation. After converting the RGB values of the image into CIELab values, one of the six measured images was used as a reference image, and the difference between the reference image and the CIELab value for the white comparison unit of each image was calculated. After applying it to the CIELab value of the pad, it was converted back to the RGB value (R'G'B '). As a result, as shown in Table 3, it was found that the deviation of the result value was improved.

division R ' G ' B ' Average Standard Deviation Average Standard Deviation Average Standard Deviation Pad 1 145 5.9 62 4.9 38 6.3 Pad 2 151 4.7 107 5.5 19 19.9 Pad 3 97 7.8 91 2.7 57 11.1 Pad 4 148 4.8 103 5.3 12 15.8

The ratio between the reference image and the R value of the red contrast means of each image, the G value of the green contrast means, and the B value of the blue contrast means was calculated using one of the images measured six times, The calculated value was applied to the value of each pad, and then converted into an RGB value (R "G" B "). As a result, as shown in Table 4, it can be seen that the deviation of the result is improved.

division R " G " B " Average Standard Deviation Average Standard Deviation Average Standard Deviation Pad 1 148 2.1 69 4.7 44 6.9 Pad 2 152 2.7 104 2.2 36 11.8 Pad 3 100 11.2 94 1.6 61 6.0 Pad 4 150 1.0 101 2.1 30 11.8

Example  2: Nitrite test

As in Example 1, the color control unit was cut into bands from the standard color table of Pantone and attached to a nitrite test strip of Merck (Germany), followed by reaction of nitrite by concentration, and the color in the reaction pad was purple . This image was obtained three times under different illuminance environments using Samsung Galaxy SII. The resulting image was averaged over three points for each pad using an image analysis program to obtain RGB values. As a result, the deviation of the RGB values was large depending on the image acquisition environment.

time One 2 3 color R G B R G B R G B 0 mg / L NO ₂ ^- 134 124 114 173 161 147 206 190 167 5 mg / L NO ₂ ^- 131 86 110 166 119 140 198 144 164 10 mg / L NO ₂ ^- 126 73 105 157 100 132 191 127 156 20 mg / L NO ₂ ^- 98 33 76 131 48 102 163 62 121 40 mg / L NO ₂ ^- 86 26 59 116 38 77 149 54 96 80 mg / L NO ₂ ^- 61 16 37 84 21 50 119 36 66

The ratio of the reference image to the R value of the red contrasting means of each image, the G value of the green contrasting means, and the B value of the blue contrasting means was calculated as one of the images measured three times, The calculated values were applied to the values of the respective pads, and then converted into RGB values (R "G" B ") as shown in the following table.

time One 2 3 color R " G " B " R " G " B " R " G " B " 0 mg / L NO ₂ ^- 134 124 114 136 136 112 138 130 109 5 mg / L NO ₂ ^- 131 86 110 131 100 106 132 98 107 10 mg / L NO ₂ ^- 126 73 105 123 84 100 128 87 102 20 mg / L NO ₂ ^- 98 33 76 103 40 78 109 42 79 40 mg / L NO ₂ ^- 86 26 59 91 32 59 100 37 63 80 mg / L NO ₂ ^- 61 16 37 66 18 38 80 25 43

The value for the color change was calculated as 1 / (R " + B ") and as a result, a quantitative result was obtained as shown in FIG.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (17)

A method of measuring an analyte in a sample comprising the steps of:
(a) a reaction device comprising (i) a color reaction means for applying a sample to induce a color reaction, and (ii) a control means for color correction having a pre- Thereby inducing a color reaction to occur in the color reaction means; Wherein the color correcting control means and the color reaction means are adjacent to each other on a single support and are positioned on a straight line on the same surface;
(b) a control-resultant synchronous image comprising a first image as an image of said color correcting means in said device and a second image as an image of a result of said color reaction to a portable terminal comprising a camera module, result synchronized image) in one image at the same time; The first image and the second image are simultaneously obtained as one image under the same light environment and the portable terminal including the camera module includes an analysis program for providing data on the presence or concentration of the analyte in the sample ;
(c) comparing the first image and the second image in the contrast-result synchronous image to calibrate the color of the second image; And
(d) obtaining data on the presence or concentration of the analyte in the sample from the color of the corrected second image.
The method according to claim 1, wherein the sample is at least one selected from the group consisting of blood, plasma, serum, urine, lymph, bone marrow, saliva, milk, ophthalmic solution, semen, brain extract, spinal fluid, joint fluid, thymus fluid, , Tap water, sewage, sewage, or ground water.
delete The method according to claim 1, wherein the color correcting control means is three or more and ten or less.
5. The method of claim 4, wherein the color correction verification means is used as a coding system.
delete The method of claim 1, wherein the portable terminal is a smart phone.
delete The method of claim 1, wherein the optical environment is under an external light source or an internal light source.
A system for measuring an analyte in a sample comprising:
(a) a reaction device comprising (i) a color reaction means for applying a sample to induce a color reaction to occur, and (ii) a control means for color correction having a pre-determined color; Wherein the color correcting control means and the color reaction means are adjacent to each other on a single support and are positioned on a straight line on the same surface;
(b) a portable terminal including a camera module; Wherein the portable terminal including the camera module is a control-result synchronous device including a first image as an image of the color-correcting control means in the reaction device and a second image as an image resulting from a color reaction in the color- Concurrently obtaining a reference-result synchronized image in one image under the same light environment; And,
(c) comparing the first image and the second image in the contrast-result synchronous image to calibrate the color of the second image and determine the presence or absence of the analyte in the sample from the color of the corrected second image Or analyzing means for providing data on the concentration; The analysis means is included in the portable terminal.
delete 11. The system according to claim 10, wherein the color correcting means is three or more and ten or less.
13. The system of claim 12, wherein the color correction verification means is used as a coding system.
delete 11. The system of claim 10, wherein the portable terminal is a smartphone.
delete 11. The system of claim 10, wherein the portable terminal including the camera module includes a light source for providing the optical environment.

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