KR101398410B1 - Method for extracting omega3 of sturgeon according to nucleic extractive conditions - Google Patents

Abstract

The present invention relates to a method for extracting omega-3 of sturgeon by nucleic acid extraction conditions, comprising: a raw material weighing process for measuring sturgeon and nucleic acid; A first mixing step of mixing the metered raw materials at 40 DEG C for 4 hours; A filtration step of filtering the mixed raw material through 100 meshes; A concentration step of removing the nucleic acid remaining in the filtered filtrate; And a second mixing step of mixing the sludge and the refined oil in the concentrated liquid. The present invention has the effect of easily extracting omega-3 of sturgeon in a nucleic acid extraction condition, which is an optimum condition for commercialization of sturgeon which is being cultivated as a locally specialized item in Hamyang County Office.

Description

TECHNICAL FIELD The present invention relates to a method for extracting omega-3 from sturgeon,

The present invention relates to a method of extracting omega-3 of sturgeon, and more particularly, to a method of extracting omega-3 of sturgeon by nucleic acid extraction conditions.

Since 2003, Hamyang County has been designing sturgeon as a specialized item to cultivate local specialties of Hamyang County. The sturgeon is a very old species that has existed since the Cretaceous period and is known as a living fossil. It is distributed in the northern mid-latitude north of the northern hemisphere (Europe, Asia, North America, etc.) and 26 species (Species) The species is endangered or endangered.

Sturgeon is a species belonging to Acipenseridae (family), Acipenser and Huso in genus, and is called Schaphirhychus speed sturgeon with Sca-phirhychinidae. Sturgeon (Sturgeon) ), And in Korea, it has been known as sturgeon, dragon shark, swordfish, and armor general.

Although the fish family is called Chordata, it is named shark, but it has a characteristic of Chondrostean fish which is totally different from the common shark (Elasmobranchi) ㆍ In case of Jonghyun species, it grows to about 2m and weighs 10 ~ 60kg. For large species, it grows up to 6m and sometimes weigh more than 1 ton. The life span is known to be around 35 years for small species, 75 to 120 years for medium species, and sturgeon more than 170 years for large species.

It is very gentle, has no teeth, and has four bees around its pointed and wide mouth inhabiting underwater. It senses crustaceans like crustaceans and small fishes, lives as a prey, and most species, like salmon, After returning to freshwater and laying eggs, I have finished my life.

More than 70% of the sturgeon is composed of cartilage. Scales have only a few whorls on the caudal fin, and the hard shell is covered on the back in five rows. One dorsal fin, two side fins, two dorsal fin , And also has a lot of eggs compared to other fish. The salted egg is known as Caviar, one of the three gourmet meals in the world.

There are 26 species of sturgeon in the world, 26 species of 2, 6, and species, and tibia fish and cartilaginous fish with three species appeared in our country, but recently are in danger of extinction.

Sturgeon sharks migrate from the sea to the freshwater area, from the river downstream to the river upstream, and spend their lives mostly in the ocean or in the jockey area. Some are adapted to freshwater and have a lifetime in pure fresh water.

The spawning season is anomalous and occurs in the spring and summer when the water temperature rises. It is located in the upper part of the river with stones, pebbles or gravel and sand. It is rich in oxygen and spawns in a place where the flow rate is fast. Times. Egg is a sedimentation scale, size varies depending on the species but usually 2 ~ 4mm in diameter.

The resources of sturgeon are mainly distributed and caught in the Caspian Sea of Russia, and 90% of the world is produced here. Due to the excellent economic value of sturgeon, we are investing heavily in the development of aquaculture, and we are producing hybrid sturgeon by cross breeding. Acipenser sinensis (Acipenser sinensis) is a fish species originated from 450 to 350 million years ago (Devonian) and belongs to the Acipenseridae family. It is widely distributed in the northern mid-latitude region and 27 species (Species) Are now known, but almost all species are endangered or endangered species. Although the fish family is called Chordata, it is named Shark because it is similar to Shark, but it has the characteristic of Chondrostean fish, which is completely different from Elasmobranchi, which is a common shark attacking people.

Since the launch of the WTO and the entry into force of the FTA, the world's agricultural and marine products have entered an age of infinite competition, one of the most active alternatives to survive in the opening up of agricultural and marine products and in the international competition system has been to develop high value-added agricultural and marine products as regional specialties. The development and commercialization of health-oriented processed foods using the functions of these agricultural and marine products has been scientifically tested and spread widely in domestic and foreign markets to establish a stable supply and demand system of agricultural products and to realize high value added of agricultural and marine products as an optimal alternative .

From this point of view, the sturgeon aquaculture business, which is being promoted since 2002 to transform Hamyang-gun into a high-income, high-function agricultural and marine products-based agricultural industry, is a very promising item. And high-value-added processed merchandising are recognized as the best results in order to expand the market. Therefore, it is anticipated that if Hamgyang sturgeon is purchased as a functional food by purchasing the full amount from Hamyang Jirisan farming association, which is a food processing company in the hall, it will have a large effect on revitalization of local economy by increasing income through commercialization and commercialization.

As a part of our efforts, we have established the extraction condition of the target functional material of the sturgeon and analyzed the possibility of developing the product to analyze it. At this time, one of the most useful components among sturdy shark's various useful components is characterized by a high content of omega-3 fatty acids such as linolenic acid, EPA (Eosine Penteonic Acid) and DHA (Docosa hexaenoic acid). These substances are effective in the prevention and treatment of various cardiovascular diseases by improving cholesterol content in the blood, and DHA is a component of brain and retina, and is useful for improving learning ability and thinking ability.

Therefore, omega - 3 was extracted from various extraction conditions as a functional material which can realize high value - added by using functional food as a by - product which removed caviar of sturgeon which was cultured at a fishery company in Hamyang - gun.

As a first method for omega 3 extraction of sturgeon, extraction method under hot water extraction condition was studied. Striped shark (4.1 kg) was cut to an appropriate size, and then 15 times water was added to the weight, and the extract solution was further extracted for 2 hours with 0.6% Brix from 5 hours after extraction to obtain an extract solution value of pH 8.89 and 0.6% Brix . That is, under the hot water extraction condition, the sample amount is 4.1 kg, the water amount is 61.5 L, the amount of the extract is 67 L, and Brix is 0.6, and the yield is 89.3%.

Herein, components of omega-3 fatty acids such as linolenic acid, DHA, EPA and selenium were analyzed in the sturgeon extract. As a result, linolenic acid 15.20 mg / g, DHA 10.58 mg / g, EPA 2.61 mg / g, and selenium 3.74 mg / g. Thus, an inspection report of the extract extracted by the first method is shown in Fig. 1A.

As a second method, a method of lyophilizing whole sturgeon into a powder was carried out. The total weight of frozen sturgeon was 4 kg and the weight was 0.41 kg. As a result, it was found that the freeze-drying yield was 9.8%.

The components of the omega-3 fatty acids in the sturgeon extracts were 0.13 mg / g of linolenic acid, 0.34 mg / g of DHA, 0.52 mg / g of EPA and 1.37 mg / g of selenium. Thus, an inspection report of the extract obtained by the second method is shown in Fig. 1B.

As a third method, the extraction under the enzymatic decomposition extraction conditions was proceeded. Enzyme decomposition extraction method is a process of measuring the amount of iron shark, enzyme, purified water to the amount of input, enzymatic decomposition at 55 ° C for 90 minutes by putting each raw material into the instrument, adding ginger after the enzymatic decomposition, , Filtration at 60 mesh, and concentration of filtrate at 50 brix. In this case, the extracted amount of sturgeon was 11.41brix at 1641ml, and the amount of concentrate was 274.01g at 50brix.

The components of omega-3 fatty acids in the sturgeon extracts were 1.62 mg / g of linolenic acid, 17.4 mg / g of DHA, 10.89 mg / g of EPA and 0.52 mg / g of selenium. Thus, an inspection report of the extract obtained by the third method is shown in Fig. 1C.

The components of the omega-3 fatty acids among the sturgeon extracts according to the three methods described above were smaller than those of the omega-3 fatty acids in the nucleic acid extraction conditions according to the present invention described below.

Therefore, a method for extracting omega-3 at a high level is required for commercialization using sturgeon shark extract. Thus, a method for extracting omega-3 of sturgeon by nucleic acid extraction conditions according to the present invention was invented.

DISCLOSURE Technical Problem The present invention has been proposed in order to solve the above problems, and it is an object of the present invention to provide a method of extracting omega-3 of sturgeon by nucleic acid extraction conditions in order to commercialize sturgeon being cultivated as a locally specialized item in Hamyang County .

Another object of the present invention is to provide a method for extracting omega-3 of sturgeon by nucleic acid extraction conditions for realizing high value-added by-products obtained by removing caviar of sturgeon as a functional food.

It is another object of the present invention to provide a method for extracting omega-3 of sturgeon by nucleic acid extraction conditions which can easily extract omega-3 among useful components of sturgeon.

It is still another object of the present invention to provide a method for extracting omega-3 of sturgeon by nucleic acid extraction conditions capable of highly extracting linoleic acid, DHA, EPA and selenium which are omega-3 fatty acids extracted from sturgeon.

It is still another object of the present invention to provide a method for extracting omega-3 of sturgeon by nucleic acid extraction conditions in which omega-3 extracted from sturgeon is well preserved, and in particular, can be preserved microbiologically.

To achieve the above object, according to the present invention, there is provided a method for extracting omega-3 of sturgeon according to a nucleic acid extraction condition, comprising: a raw material weighing process for measuring sturgeon and nucleic acid; A first mixing step of mixing the metered raw materials at 40 degrees for 4 hours; A filtration step of filtering the mixed raw material through 100 mesh; A concentrating step of removing the nucleic acid remaining in the filtered solution; And a second mixing step of mixing the sludge and the refined oil in the concentrated liquid. Herein, the refined oil refers to a lipophilic substance remaining after filtration of the sturgeon with a nucleic acid as a solvent under a predetermined condition and then removing the nucleic acid from the extract. The sludge can not pass through the filter during filtration during the production of the sturgeon, It says.

The raw material measuring process of the omega-3 extraction method of sturgeon according to the nucleic acid extraction condition according to the present invention is characterized in that the mixing ratio of the sturgeon and nucleic acid is 16.67% and 83.33%, and the dosage is 800.16 g and 3999.84 g, respectively It is characterized by.

The present invention has the effect of easily extracting omega-3 of sturgeon in a nucleic acid extraction condition, which is an optimum condition for commercialization of sturgeon which is being cultivated as a locally specialized item in Hamyang County Office.

Also, byproducts from the caviar of sturgeon can be used as functional foods to realize high added value.

In addition, omega-3 can be easily extracted from useful components of sturgeon, and linoleic acid, DHA and EPA, which are omega-3 fatty acids, can be extracted with high efficiency.

We also examined the effect of omega - 3 extracted from sturgeon, especially microbiologically, on the preservation of extract.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art. Accordingly, the true scope of protection of the present invention should be determined only by the appended claims.

FIGS. 1A to 1C are Omega 3 extracts of sturgeon according to the prior art, which are analyzed by the Korea Functional Food Research Institute.
FIG. 2 is a flowchart showing a method for extracting omega-3 of sturgeon by nucleic acid extraction conditions according to the present invention.
FIG. 3 is a component test report analyzing Omega 3 extract of sturgeon according to the nucleic acid extraction condition according to the present invention by Korea Functional Food Research Institute.

Hereinafter, a method for extracting omega-3 of sturgeon according to a nucleic acid extraction condition according to a preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings. FIG. 2 is a flow chart showing a method of extracting omega-3 of sturgeon according to a nucleic acid extraction condition according to the present invention, and FIG. 3 is a diagram illustrating a method of extracting omega-3 extract of sturgeon by nucleic acid extraction conditions according to the present invention It is a component inspection report.

Referring to FIG. 2, in the method for extracting omega-3 of sturgeon according to the nucleic acid extraction condition according to the present invention, a raw material weighing process for measuring sturgeon and nucleic acid is performed (S1). Here, the mixing ratio of sturgeon and nucleic acid is set to 16.67% and 83.33%, and the amounts of the ingredients are set to 800.16 g and 3999.84 g, respectively.

Next, a first mixing process of mixing the metered raw materials at 40 degrees for 4 hours is performed (S3).

Subsequently, the mixed raw material is filtered through 100 meshes (S5), and a concentrated process for removing the nucleic acid remaining in the filtered solution is performed (S7).

Next, a second mixing process of mixing the sludge and the refined oil is performed in the concentrated liquid, and the extracted liquid comes out (S9).

Analysis of omega-3 fatty acids in the extract of sturgeon extracted with the present invention showed that the contents of linolenic acid 3.51 mg / g, DHA (docosa hexaenoic acid) 24.90 mg / g, EPA (eosine penteonic acid) 15.31 mg / g, selenium 0.34 mg / g. Thus, the inspection report of the extract obtained by the extraction method according to the present embodiment is shown in Fig.

Table 1 below shows the analysis of the components of the omega-3 fatty acids of the sturgeon extract according to the three extraction methods and the extraction method according to the present invention, which are the background of the above-described invention.

Test Items Hot water extraction Freeze-dried Enzymatic decomposition extraction Nucleic acid extraction Linolenic acid 15.20 0.13 1.62 3.51 DHA 10.58 0.34 17.4 24.90 EPA 2.61 0.52 10.89 15.31 Selenium 3.74 1.37 0.52 0.34

As shown in Table 1, omega-3 fatty acids were extracted from the omega-3 extraction method of sturgeon according to the nucleic acid extraction conditions according to the present invention, as compared with the above-mentioned three conventional methods.

Of the omega-3 fatty acids, EPA and DHA are highly unsaturated fatty acids. EPA is known to be effective for cardiovascular-related diseases such as improving cholesterol content in the blood and promoting blood flow. DHA is known to be a component of the brain and retina Therefore, it is known as an effective ingredient for improving learning ability and improving visual acuity. In case of sturgeon, EPA and DHA are abundant, and the content of essential fatty acid is abundant, so it is excellent in nutrition.

As shown in the above table 1, the functional products targeting the omega-3 fatty acids of sturgeon are not extracted by heat extraction, lyophilization, enzymatic digestion, When analyzed, many polyunsaturated fatty acids of EPA and DHA are extracted. Therefore, it was shown that the second commercialization using omega-3 fatty acids of EPA and DHA extracted by nucleic acid extraction method was most effective.

Meanwhile, a microbiological study was carried out for the preservation of the extract extracted with the omega 3 extraction method of sturgeon according to the nucleic acid extraction condition according to this embodiment.

The salt used in this study was a comparative salt of reagent (Merck KGaA, Darmstadt, Germany), and bamboo salt was used in the experiment. Porphyromonas was used. The medium used in this experiment was purchased from Becton Dickeson (Sparks, Md. USA) as Leuria bertani (LB) broth and agar was purchased from Merck.

The salt of the reagent and the salt of bamboo salt were dissolved in 10 mg of tertiary distilled water at 30% salinity (w / v), and centrifuged at 1000 mg at room temperature for 10 minutes. Then, LB agar solid and broth liquid medium Were used to test the antibacterial and bactericidal action of the samples against the general microorganism group.

Of the four strains used in this study, Escherichia coli was distributed from ATCC, and Enterococcus faecalis, Pseudomonas aeroginosa, and staphylococcus aureus were purchased from the Department of Microbiology, University Hospital B.

The microorganisms were tested three times to evaluate the bactericidal efficacy of the samples. The results of the experiments on each microorganism are as follows.

(Bactericidal effect of various salts concentration (w / v) against a Staphylococcus aureus) Salt s 5% 6% 7% 8% 9% 10% 15% 16% 20% Reagent
salt (merck) 3+ 3+ 2+ 2+ 1+ 1+ 0 0 0 0 Salt + sturgeon
fluid 3+ 2+ 2+ 2+ 2+ 2+ 2+ 1+ 0 0 Bamboo salt + sturgeon fluid 3+ 2+ 1+ 1+ 1+ 0 0 0 0 0

Bactericidal effect of various concentrations of salt on Pseudomonas aeruginosa (Bactericidal effect of various salts concentration (w / v) against a Pseudomonas aeruginous) Salt s 5% 6% 7% 8% 9% 10% 15% 16% 20% Reagent
salt (merck) 3+ 3+ 2+ 2+ 1+ 1+ 0 0 0 0 Salt + sturgeon
fluid 3+ 2+ 2+ 1+ 0 0 0 0 0 0 Bamboo salt + sturgeon fluid 3+ 1+ 0 0 0 0 0 0 0 0

Bactericidal effects of various concentrations of salt on intestinal bacteria (a / b) against a Enterococcus facials Salt s 5% 6% 7% 8% 9% 10% 15% 16% 20% Reagent
salt (merck) 3+ 2+ 2+ 2+ 1+ 0 0 0 0 0 Salt + sturgeon
fluid 3+ 2+ 2+ 1+ 0 0 0 0 0 0 Bamboo salt + sturgeon fluid 3+ 2+ 1+ 0 0 0 0 0 0 0

(Bactericidal effect of various salts concentration (w / v) against Escherichia coli) Salt s 5% 6% 7% 8% 9% 10% 15% 16% 20% Reagent
salt (merck) 3+ 2+ 2+ 2+ 1+ 1+ 0 0 0 0 Salt + sturgeon
fluid 3+ 2+ 2+ 1+ 1+ 0 0 0 0 0 Bamboo salt + sturgeon fluid 3+ 1+ 0 0 0 0 0 0 0 0

The minimum inhibitory concentration (MIC) of various salts against bacteria on solid agar media (% w / v) Intestinal Bacteria  Salt (%) Salt + sturgeon fluid (%) Bamboo salt (%) +
정성 유유 Escherichia coli 10 8 6 Pseudomonas
aeruginous 10 8 6 Staphylococcuc
aureus 10 16 9 Enterococcus facials 9 8 7

As shown in the above tables, E. coli began to exhibit a bactericidal effect at 5% salinity and at 5% salinity at 8% salinity, and at 5% in medium containing bamboo salt and sturgeon extract And completely disappeared at 6%.

Staphylococcus aureus began to show bactericidal effect in 8% of salmon and salmon extracts, and was completely extinguished in 16%. In the medium containing bamboo salt and sturgeon extract, the bacteria started to die at 5% and the bacteria completely died at 9%.

Pseudomonas aeruginosa showed a bactericidal effect in 5% of medium mixed with sea salt and sturgeon extract, and completely disappeared in 8%. In the medium containing bamboo salt and sturgeon extract, bactericidal effect was shown in 5% and 6% It was completely annihilated.

Intestinal bacteria began to exhibit bactericidal effect in 5% of medium mixed with sun salmon and sturgeon extract, and 8% of bacteria showed extinction in 5% of medium mixed with bamboo salt and sturgeon extract. 7%.

The results of the study on the antimicrobial sterilization effect when the bamboo salt and the sturdy shark extract were mixed with the culture medium of the common microorganism and the bamboo salt mixed with the sturgeon extract, the following results are obtained.

EPA and DHA, which are omega-3 fatty acids which are unsaturated fatty acids, are easy to oxidize. Therefore, the addition of 6% bamboo salt and 8% sodium chloride to the microbial stability test for the preservation of sturgeon shark extract targeted to omega-3 fatty acid alleviates oxidation of omega-3 fatty acids, EPA and DHA.

On the other hand, bamboo salt is effective for various diseases and is mainly used for the treatment of digestive or antidote, inflammation and ulcer, trauma and hemorrhage. In this experiment, it can be seen that microorganisms have much sterilization effect when mixed with 6% bamboo salt mixed with sturgeon extract.

These results suggest that microbial strains have better antioxidative and antimicrobial effects when mixed with bamboo salt and bamboo salt rather than salted and salted shark extract.

Meanwhile, the method for extracting omega-3 of sturgeon according to the nucleic acid extraction condition according to the present embodiment is not limited to the above-described embodiment, and various modifications may be made without departing from the technical spirit of the present invention. It will be readily apparent to those skilled in the art to which the present invention pertains.

Claims (2)

A raw material weighing process for weighing sturgeon and nucleic acid;
A first mixing step of mixing the metered raw materials at 40 degrees for 4 hours;
A filtration step of filtering the mixed raw material through 100 mesh;
A concentrating step of removing the nucleic acid remaining in the filtered solution;
In the concentrated liquid, the sturgeon is extracted with a solvent of nucleic acid as a solvent under a certain condition, and then purified oil, which is the remaining fat-soluble material after the nucleic acid is removed from the extract, and sludge which is filtered without passing through the filter during the production of sturgeon- And a second mixing step of mixing the omega-3 and omega-3 extracts. The method according to claim 1,
Wherein the mixing ratio of the sturgeon and nucleic acid is 16.67% and 83.33%, and the amount of the fish is 800.16 g and 3999.84 g, respectively.

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