KR101383519B1

KR101383519B1 - Composition having excellent antibacterial effect and manufacturing method thereof and cosmetic composition comprising thereof

Info

Publication number: KR101383519B1
Application number: KR20120156370A
Authority: KR
Inventors: 최화선; 신봉기
Original assignee: 최화선
Priority date: 2012-12-28
Filing date: 2012-12-28
Publication date: 2014-04-08

Abstract

The present invention relates to a composition having an excellent antibacterial effect, a manufacturing method thereof, and a cosmetic composition comprising the same and, more specifically, to a cosmetic composition which has an excellent wide range of antibacterial activity against various bacteria causing skin diseases, has an excellent moisture supply ability, has a skin whitening effect, has an anti-aging effect via excellent antioxidant effects, minimizes skin irritation by using natural ingredients, and facilitates long-term storage by having an excellent antiseptic ability.

Description

Composition having excellent antimicrobial effect, a method of manufacturing the same and a cosmetic composition comprising the same {Composition having excellent antibacterial effect and manufacturing method

The present invention relates to a composition having an excellent antimicrobial effect, a method for producing the same and a cosmetic composition comprising the same, and more particularly, to a cosmetic composition having excellent antibacterial action and excellent moisture supply power and excellent antiseptic power and easy storage for a long time. will be.

In general, human skin is the most important organ that has the biochemical and physical functions to protect the inside of the human body. Skin can be divided into three parts: epidermis, dermis and hypodermis.

As the skin ages, typical symptoms of aging such as wrinkles, blemishes, and brown mushrooms appear due to ultraviolet rays, wounds, and environmental pollution. Generally, there are many theories explaining skin aging, but among them, the free radical theory that the skin ages due to free radicals caused by various factors in and out of the body is accepted as the most valid theory.

Free radicals are chemically highly reactive chemicals that occur when the skin is irradiated with UV light or during the respiration of cells in the skin. The peroxidation of lipids caused by them generates various active species such as radicals, peroxides, aldehydes, epoxides, and the like, and damages DNA, RNA, proteins, cell lines and cellular structures.

Since free radicals or reactive oxygen species are related to skin aging, conventionally, superoxide dismutase, tocopherol, ascorbic acid, glutathione, and the like have been formulated in cosmetics to prevent skin aging. When the above components are combined, the product stability is poor, or the solubility is poor, and it is difficult to obtain a practical effect due to the concentration limitation in the actual product application.

In addition, since the skin is a part of direct contact with the surrounding environment, infections such as bacteria and fungi easily occur, and are Gram-positive bacteria Staphylococcus sp., Streptococcus sp. And Gram-negative bacteria. Pseudomonas aeruginosa, Escherichia coli, and fungi are among the most common species found in Staphylococcus, Staphylococcus epidermidis, Staphylococcus aureus and Propionibacterium acnes. These bacteria infect the skin and cause skin diseases such as acne, rashes, ringworm, swelling, and swelling. Conventionally, chemical bactericides such as Triclocarban and Triclosan have been used for the purpose of sterilizing these bacteria, but they are relatively ineffective against Gram-negative bacteria and fungi, causing resistant bacteria and causing skin irritation. The moisturizing power was so low that the use was limited.

The present invention has been made to solve the above problems, the object of the present invention is excellent in a wide range of antimicrobial activity against various bacteria causing skin diseases, excellent water supply, skin whitening effect, excellent antioxidant effect Through to provide a cosmetic composition having a skin anti-aging function. In addition, by using a natural material to minimize the skin irritation while providing an excellent antiseptic power cosmetic composition easy to store for a long time.

In order to solve the above problems, the present invention,

It provides an excellent antimicrobial composition containing Bupyeongcho extract, Echoseongcho extract, ear cow extract and alum as an active ingredient.

According to a preferred embodiment of the present invention, the alum may be heat-treated for 10 to 20 minutes at 150 to 350 ℃.

According to another preferred embodiment of the present invention, the composition may comprise 0.0001 to 80% by weight of the active ingredient.

According to another preferred embodiment of the present invention, the composition may include 20 to 50 parts by weight of Bupyeongcho, 20 to 50 parts by weight, 20 to 50 parts by weight of alum, and 20 to 50 parts by weight of antelope based on 100 parts by weight of alum.

In addition, the present invention comprises the steps of (1) extracting the extract of Bupyeongcho, eoseongcho and jeonwoowoo; (2) mixing 20 to 50 parts by weight of Bupyeongcho, 20 to 50 parts by weight, and 20 to 50 parts by weight of precious cows with respect to 100 parts by weight of alum; provides an excellent antimicrobial effect.

The present invention also provides a cosmetic composition containing the composition described above.

According to a preferred embodiment of the present invention, the cosmetic composition is an external skin ointment, face wash, softening lotion, nourishing longevity, skin, lotion, nourishing lotion, nourishing cream, massage cream, mist, essence, pack, emulsion, or oil gel It may be a formulation of.

Composition excellent in antimicrobial effect of the present invention, a method for producing and a cosmetic composition comprising the same have excellent antibacterial activity against a variety of bacteria causing skin diseases, excellent moisture supply, skin whitening effect, excellent antioxidant effect Through the anti-aging function of the skin, by using a natural material can provide a cosmetic composition that is easy to store for a long time with excellent antiseptic power while minimizing skin irritation.

Hereinafter, the present invention will be described in more detail.

As described above, there has been a problem of the appearance of resistance-derived strains or skin irritation due to the use of a chemical synthetic fungicide, a problem of not only insufficient antibacterial activity against various bacteria causing skin diseases, but also a significant drop in skin moisturizing function. there was. In addition, when using a natural material to minimize skin irritation there was a problem that the long-term storage is difficult to prevent the preservation.

Accordingly, the present invention sought to solve the above problems by providing a composition having excellent antibacterial effect containing Bupyeongcho, Echochocho and Ear Chuncho extract and alum as an active ingredient. Through this, it has excellent antibacterial activity against various bacteria causing skin diseases, excellent moisture supply, skin whitening effect, anti-aging effect through excellent antioxidant effect, and minimizes skin irritation by using natural materials At the same time, it is easy to store for a long time due to its excellent antiseptic property.

Specifically, the Bupyeongcho (Spirodela polyrhiza) is a perennial herb belonging to the Lemnaceae family, in oriental medicine, the whole plant is used for the treatment of antipyretic, diuretic and nosebleeds in the sun, as a medicine to pee, sweat, and steamed I use it. Houttuynia cordata is a perennial herb that belongs to more than three hundred, and it is known to be effective in treating pneumonia, edema, disease, eczema, etc. by detoxification, diuresis, and sarcoma. In addition, the jeonwoowoo refers to the arrowhead (Euonymus alatus) belonging to the genus Nova, it is known as a medicine that is effective in bruising, pain caused by bruises, bleeding due to the swelling action.

It was confirmed that the composition of the present invention containing the active ingredient mixed with the extract of Bupyeongcho, Echoseong and Haewoowoowoo Alum has excellent antibacterial effect against various bacteria. Therefore, the composition of the present invention can be used as a cosmetic composition for preventing skin diseases and anti-inflammatory by bacterial infection with excellent antimicrobial action against various bacteria, and can be used as a substitute for preservatives in the production of processed meat products by appropriate amount of edible. It can also be used for kitchen detergents.

At this time, the alum (AlK (SO ₄ ) _{2 ·} 12H ₂ O) is preferably a heat treatment can be used. By using heat-treated alum, it is possible to remove impurities in minerals to obtain pure crystals.Decreased function of destroying cell walls of bacteria by the same weight by dehydration of alum increases the chance of recombination with other liquid compositions. It has the advantage of providing. Alum heat treatment may be carried out at 150 to 350 ℃ for 10 to 20 minutes. If the heat treatment temperature of the alum is less than 150 ℃, the increase of antimicrobial power is insufficient, and it takes a long time to be combined with other compositions, which reduces the work efficiency and has the disadvantage of limiting the addition of functions other than sterilization power. It is difficult to try to combine with other natural products to add functions other than bactericidal power, there is a problem that the skin moisturizing power is significantly lowered, and the effect of improving the antimicrobial power is not increased further as the temperature increases, but is maintained at a certain level. have.

The excellent antimicrobial effect of the present invention may comprise 0.0001 to 80% by weight of the active ingredient. The solvent for dissolving the active ingredient is not particularly limited as long as it does not interfere with the effects of the present invention, and more preferably water, ethanol, propyl alcohol, or the like. If the content of the active ingredient is less than 0.0001% by weight it is difficult to implement the effect, such as antimicrobial activity, if it exceeds 80% by weight may have a problem in formulation stability.

In addition, the active ingredient may include 20 to 50 parts by weight of Bupyeongcho, 20 to 50 parts by weight, and 20 to 50 parts by weight of woojeonwoo with respect to 100 parts by weight of alum. If the Bupyeongcho is less than 20 parts by weight, antimicrobial activity is reduced, water supply is also reduced, and it is less effective to alleviate the symptoms of itching and pests such as mosquitoes. Rather, there is a problem that the sterilization ability is weakened, when the fish paste is less than 20 parts by weight, the function of reinforcing the sterilization ability of the alum is inferior, and the efficacy of the moisturizing power is also difficult to expect. There is a difficulty that can cause the user to reject, there is a problem that the function of Bupyeongcho weakened. If the ear cow is less than 20 parts by weight, there is a disadvantage that the antioxidant function is reduced, if it exceeds 50 parts by weight it is difficult to determine the efficacy of the increase in content there is a problem of only the burden of cost increase.

Next, a method of preparing the composition of the present invention is provided.

The method for preparing a composition of the present invention comprises the steps of: (1) extracting and extracting Bupyeongcho, Echochocho, and Chunwoowoo; And (2) mixing 20 to 50 parts by weight of Bupyeongcho, 20 to 50 parts by weight, and 20 to 50 parts by weight of precious cows based on 100 parts by weight of alum.

Bupyeongcho, Echochocho, and Jeonwoowoo Powder powder containing about 1 to 30 times, preferably about 1 to 15 times, water, ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, butylene glycol, propylene glycol, etc. It can be extracted using a single or mixed solvent of, more preferably hot water extraction at 60 to 100 ℃. The extracted extract can be concentrated by filtration and freeze-dried to obtain an extract.

The extract is mixed with 20 to 50 parts by weight of Bupyeongcho, 20 to 50 parts by weight, and 20 to 50 parts by weight of precious cows based on 100 parts by weight of alum. At this time, the alum is important to undergo a heat treatment process, there is an advantage that can be obtained pure crystals by removing impurities in the mineral through the heat treatment, the function of destroying the cell wall of bacteria to the same weight by the dehydration of alum. It has the effect of increasing the chance of recombination with other liquid compositions. The heat treatment of the alum is preferably carried out for 10 to 20 minutes at 150 to 350 ℃, the increase in antimicrobial power is insufficient when the alum heat treatment temperature is less than 150 ℃, work takes a long time to compound with other compositions There is a disadvantage in that there is a limit to the addition of functions other than bactericidal power due to poor efficiency, it is difficult to attempt to combine with other natural products to add functions other than bactericidal power when the temperature exceeds 350 ℃, there is a problem that the skin moisturizing power is significantly lowered, antibacterial activity There is a drawback that the effect of the enhancement is not increased further as the temperature is increased but is maintained at a constant level.

In addition, the present invention provides a cosmetic composition comprising a composition having excellent antimicrobial effect. The cosmetic composition of the present invention can secure sufficient skin moisturizing power while having an excellent antibacterial effect on bacteria causing skin diseases. In addition, it has an excellent antioxidant effect, has an anti-aging function, and is effective in whitening, nutrition, and the like.

The cosmetic composition of the present invention may be blended with the above essential components, if necessary, with other ingredients normally formulated into cosmetics. Other ingredients that can be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings. , Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

The cosmetic composition may be prepared in any formulation commonly prepared in the art, preferably skin external ointment, face wash, softening longevity, nourishing longevity, skin, lotion, nutrient lotion, nourishing cream, massage cream, essence, Formulations such as packs, emulsions, oil gels, and the like.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples should not be construed as limiting the scope of the present invention, and should be construed to facilitate understanding of the present invention.

&Lt; Examples 1 to 6 >

Each 1kg of dried Bupyeongcho, Eoseongcho and Chunjeonwoo were finely ground using a blender, and then sieved through a 0.5mm sieve, and then the powder was used. After distilled water of about 10 times the volume of the powder sample was added, the reflux condenser (Eyela Srs-651, Japan) was attached, and the extract was extracted at 100 ° C. for 1 hour, and then filtered in a freeze dryer (Ilshin Lab Co.Ltd, Korea). Lyophilized.

The above Bupyeongcho, Echochocho, and Jeonwoowoo extracts per 100 parts by weight of Alum (AlK (SO ₄ ) _{2 ·} 12H ₂ O) were mixed in the mixing ratios of Table 1, and 30 wt% of the solvent was dissolved in ethanol to prepare a composition.

&Lt; Examples 7 to 12 >

Except that the alum was heat-treated for 15 minutes at 300 ℃ was prepared in the same manner as in Example 1.

&Lt; Comparative Example 1 &

It was prepared in the same manner as in Example 1 except that Bupyeongcho was not included.

&Lt; Comparative Example 2 &

It was prepared in the same manner as in Example 1 except that the fish paste was not included.

&Lt; Comparative Example 3 &

It was prepared in the same manner as in Example 1 except that no ear cow was included.

Alum (part by weight) Bupyeongcho (Weight) Eoseongcho (part by weight) Ear cow (part by weight) Example 1 100 (heat treatment x) 30 30 30 Example 2 100 (heat treatment x) 35 20 25 Example 3 100 (heat treatment x) 10 30 30 Example 4 100 (heat treatment x) 5 30 30 Example 5 100 (heat treatment x) 30 5 30 Example 6 100 (heat treatment x) 30 30 5 Example 7 100
(300 ℃ heat treatment) 30 30 30 Example 8 100
(300 ℃ heat treatment) 35 20 25 Example 9 100
(300 ℃ heat treatment) 10 30 30 Example 10 100
(300 ℃ heat treatment) 5 30 30 Example 11 100
(300 ℃ heat treatment) 30 5 30 Example 12 100
(300 ℃ heat treatment) 30 30 5 Comparative Example 1 100 (heat treatment x) 0 30 30 Comparative Example 2 100 (heat treatment x) 30 0 30 Comparative Example 3 100 (heat treatment x) 30 30 0

Experimental Example 1 Measurement of Antimicrobial Activity

Bacteria used in the antimicrobial activity experiments of the compositions prepared in Examples 1 to 12 and Comparative Examples 1 to 3 are pathogenic bacteria, Staphylococcus aureus KCMC 11593, Staphylococcus epidermidis KCTC 1917 and Gram-negative bacteria Escherichia coli KCCM 11234, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium ATCC 11862 were used. In order to investigate their antimicrobial activity, each cell was precultured in nutrient broth (Difco Co., USA).

The composition prepared according to Examples and Comparative Examples was filtered and filtered with a 0.45 μm membrane filter (Milipore Co., USA), and 100 μl of each strain was uniformly dispensed into sterile Mueller Hinton agar (Difco Co., USA) medium. After application, sterile disk (φ 8mm) was adhered to the medium at regular intervals. The diluted composition was dropped on a disk, and then cultured in an incubator at 37 ° C. for 18 hours, and the diameter of the inhibitor was measured in mm to compare the antimicrobial activity. At this time, gentamicine (20 μg) was used as a control. Table 2 shows the inhibition zone (inhibition zone) of each strain by the composition.

Experimental Example 2 Antioxidant Activity Measurement

DPPH (α-α-Diphenyl-β-picrylhydrazyl) electron donating ability was measured according to Blois (1958) method. To measure the antioxidant activity, 200 μl of the composition was mixed well with 800 μl of 100 mM Tris-HCl (pH 7.4), and then vortexed with 1 ml of 500 μM α-α-Diphenyl-β-picrylhydrazyl in MeOH solution. The reaction was then allowed to stand at room temperature for 20 minutes, filtered (0.45 μm) and absorbance measured at 517 nm with a Spectropotometer (Shimadzu Co., UV-1601, Japan). At this time, blank was used for each solvent pure instead of the sample solution.

Antioxidant activity was indicated by the concentration of the sample on the blank, and the control group was butylated hydroxy anisole (BHA); Sigma Co.) and butylated hydroxy toluene (BHT); Sigma Co.) was used. It was calculated using the following formula.

DPPH radical scavenging rate (%) = {1-(sample O. D / blank O. D)} X 100

Experimental Example 3 Tyrosinase Inhibitory Activity

Tyrosinase activity inhibitory activity was measured in 0.2 ml of 0.175 M phosphate buffer (pH 6.8) and 0.2 ml of 5 mM L-DOPA solution pre-adjusted in a 35 ° C. water bath and 0.5 ml mixture of the composition prepared according to Examples or Comparative Examples. 110 units / ml Sigma) 0.1 ml was added. After reacting for 2 minutes in a 35 ℃ water bath, the absorbance at 475 nm (Sabs) and 0.1 ml of distilled water was added instead of the enzyme solution (Babs), and 0.5 ml of distilled water was added instead of the extracted sample solution. The absorbance was calculated by the following equation using the measured values (Cabs).

Inhibition effect (%) = 1-((Sabs-Babs) / Cabs) × 100

S = enzyme solution + sample + L-DOPA (buffer + L-DOPA + sample + enzyme)

B = D. W + sample + L-DOPA (buffer + L-DOPA + sample + D. W)

C = enzyme solution + D. W + L-DOPA (buffer + L-DOPA + extractant + enzyme)

Gram-positive bacteria Gram-negative bacteria S.aureus S. epidermidis E. coli P.aeruginosa S.typhimurium Example 1 15 mm 11 mm 11 mm 15 mm 9.5 mm Example 2 16 mm 10 mm 10.5 mm 12 mm 10 mm Example 3 13.5 mm 10.5 mm 10.5 mm 12.5 mm 10 mm Example 4 13 mm 9.5 mm 10 mm 10 mm 9.5 mm Example 5 13 mm 10 mm 10 mm 11 mm 9 mm Example 6 13.5 mm 10.5 mm 10 mm 10.5 mm 9.5 mm Example 7 18 mm 12 mm 14 mm 15 mm 12 mm Example 8 17 mm 13 mm 15 mm 13 mm 11 mm Example 9 14.5 mm 12 mm 11,5 mm 11.5 mm 10 mm Example 10 12 mm 10.5 mm 10.5 mm 9.5 mm 9 mm Example 11 13.5 mm 12 mm 12 mm 12 mm 10.5 mm Example 12 14 mm 11 mm 12 mm 12.5 mm 10 mm Comparative Example 1 - 4 mm 5.5 mm - 6 mm Comparative Example 2 11 mm 9.5 mm 10 mm 8 mm 8.5 mm Comparative Example 3 10.5 mm 9 mm 10 mm 8.5 mm 10 mm Gentamicine 20 mm 14 mm 15 mm 12 mm 15 mm

As in the above results, the composition according to the present invention was confirmed to have excellent antimicrobial activity on the skin flora.

DPPH radical scavenging rate (%) Inhibition effect (%) Example 1 69.9 80.1 Example 2 70.5 80.3 Example 3 70.9 83.4 Example 4 69.5 85.2 Example 5 70.9 78.7 Example 6 69.0 79.6 Example 7 72.3 82.3 Example 8 71.9 85.0 Example 9 71.2 88.2 Example 10 70.6 89.1 Example 11 71.0 78.8 Example 12 69.8 83.3 Comparative Example 1 63.3 76.5 Comparative Example 2 68.8 62.8 Comparative Example 3 69.1 78.1

As in the above results, the composition according to the present invention was confirmed to have excellent antioxidant effect and whitening function.

In another embodiment of the present invention, a cosmetic composition comprising the composition of the embodiment excellent in the antimicrobial effect was prepared.

Manufacturing example 1: Preparation of Essence

4.00 wt% ethyl alcohol

Methyl Paraben 0.10 wt%

Incense 0.10% by weight

0.60% by weight of hydrotreated castor oil

DL-α-tocopheryl acetate 0.02 wt%

Glycerin 5.00 wt%

1,3-butylene glycol 3.00 wt%

0.20% by weight of hydroxyethyl cellulose

Xanthan Gum 0.40 wt%

E.D.T.A-2Na 0.02 wt%

Fish-Collagen 4.00 wt%

0.5-2.0 wt% of the compositions of Examples 1-12

Amount of distilled water

Manufacturing example 2: nutrition cream

Glyceryl Monostearate Lipo 2.00 wt%

Sorbitan monostearate 1.00 wt%

Polysorbate 60 1.50 wt%

Stearic acid 1.00 wt%

2.00 wt% stearyl alcohol

6.00 wt% squalene

Cetyl Octanoate 2.00 wt%

Shea Butter 1.00 wt%

0.50% by weight of methyl polysiloxane

0.10 wt% ethyl paraben

Glycerin 5.00 wt%

1,3-butylene glycol 3.00 wt%

EDTA-2Na 0.02 wt%

Methyl Paraben 0.20 wt%

0.5-2.0 wt% of the compositions of Examples 1-12

Amount of distilled water

Manufacturing example 3: whitening pack

15.0 wt% polyvinyl alcohol

Carboxymethylcellulose sodium 0.3% by weight

Concentrated glycerin 3.0 wt%

Allantoin 0.1 wt%

0.01% by weight of ethylenediaminetetraacetate acetate

Polyethylene glycol 1.0 wt%

Ethanol 6.0 wt%

0.15% by weight of methyl paraoxybenzoate

Taraoxybenzoic acid propyl 0.05% by weight

Dipanthenol 0.1 wt%

0.5 weight% of polyoxyethylene (12) nonylphenyl ether

45.0 wt% of the compositions of Examples 1-12

A small amount of spices

Purified water balance

Manufacturing example 4: Cleansing Foam Composition

Stearic acid 6.5 wt%

Myristic acid 28.0 wt%

3.0% by weight of self-emulsifiable glycerin monostearate

Propylene Glycol 5.0 wt%

Concentrated glycerin 10.0 wt%

Sodium hydroxide 7.0 wt%

0.1% by weight of ethylenediaminetetraacetate acetate

15.0 wt% of the compositions of Examples 1-12

A small amount of spices

Purified water balance

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims. Of course.

Experimental Example 4 Evaluation of Moisturizing Power

In order to evaluate the moisturizing power of the cosmetic composition, the essence cosmetic composition containing the compositions of Examples 1 to 12 and Comparative Examples 1 to 3 was applied to the skin in a quantitative manner, and then the skin's electrical conductivity was measured to measure skin moisture retention ability. It was. In the test method, the essence cosmetic composition was applied in a constant temperature and humidity room of 22 ° C. and a relative humidity of 45% by applying a certain amount (0.03 g / 16 cm 2) to the inside of each of the 20 test subjects, followed by rubbing well. Measured. As a measuring device, a skin moisture content measuring device (Skicon 200) measuring electrical conductivity of skin was used.

2 hours after application
Skin Conductivity (μ / Ω) Example 1 33.7 Example 2 35.6 Example 3 32.7 Example 4 30.8 Example 5 29.6 Example 6 30.5 Example 7 40.5 Example 8 36.7 Example 9 33.9 Example 10 35.2 Example 11 33.2 Example 12 30.1 Comparative Example 1 10.8 Comparative Example 2 15.2 Comparative Example 3 13.4

As in the above results it can be confirmed that the cosmetic composition according to the present invention shows excellent moisturizing power.

Claims

Bupyeongcho extract, Eoseongcho extract, ear woowoo extract and an antimicrobial effect containing alum as an active ingredient.

The method of claim 1,
The alum is an excellent antimicrobial effect, characterized in that the heat-treated for 10 to 20 minutes at 150 to 350 ℃.

The method of claim 1,
The composition is excellent in antimicrobial effect, characterized in that it comprises 0.0001 to 80% by weight of the active ingredient.

The method of claim 1,
The composition is an excellent antimicrobial effect, characterized in that it comprises 20 to 50 parts by weight of Bupyeongcho, 20 to 50 parts by weight, 20 to 50 parts by weight of precious cow relative to 100 parts by weight of alum.

(1) extracting and drying the Bupyeongcho, Echoseong and Haeunwoo cows after extracting the extract;
(2) mixing 20 to 50 parts by weight of Bupyeongcho, 20 to 50 parts by weight, and 20 to 50 parts by weight of precious cows with respect to 100 parts by weight of alum.

6. The method of claim 5,
The alum is a method for producing an excellent antimicrobial effect, characterized in that the heat treatment for 10 to 20 minutes at 150 to 350 ℃.

Cosmetic composition containing the composition of any one of Claims 1-4.

The method of claim 7, wherein
The cosmetic composition is a cosmetic composition, characterized in that the external cosmetic ointment, face wash, softening longevity, nourishing cosmetics, skin, lotion, nutrition lotion, nutrition cream, massage cream, mist, essence, pack, emulsion, or oil gel formulation.