KR101331677B1

KR101331677B1 - 스트레스 내성 식물

Info

Publication number: KR101331677B1
Application number: KR1020077009208A
Authority: KR
Inventors: 블록 마크 드; 마이클 메츨라프; 베로니크 고셀르
Original assignee: 바이엘 크롭사이언스 엔.브이.
Priority date: 2004-09-24
Filing date: 2005-09-16
Publication date: 2013-11-26
Also published as: US9534230B2; AU2005287499A1; CN101040049B; US20110131672A1; ZA200701725B; DE602005018379D1; WO2006032469A2; AU2005287499B2; DK1794306T3; AR056256A1; ES2338443T3; KR20120138840A; KR20070060128A; CA2581257C; WO2006032469A3; CA2581257A1; SI1794306T1; BRPI0517331A; EP1794306B1; CY1109892T1

Abstract

식물 및 식물 세포에서 스트레스 허용성은 예를 들어 식물에서의 과발현의 경우 NAD 회수 합성 경로 및/또는 NAD 신생 합성 경로에 수반되는 효소들을 암호화하는 뉴클레오타이드 서열을 사용하여 성취된다.

Description

스트레스 내성 식물{STRESS RESISTANT PLANTS}

NAD 회수(salvage) 합성 경로 및/또는 NAD 신생(de novo) 합성 경로에 수반되는 효소를 식물에서 발현시킴으로써 식물 및 식물 세포에서 스트레스 내성을 증가시키는 방법을 제공한다.

불리한 생장 조건, 예를 들어 가뭄, 강한 광 강도, 고온, 영양소 제한, 염수 생장 조건 등에 대한 식물의 허용성은 궁극적으로 상기 식물의 실제 수확량을 증가시키고자 하는 끝없는 추구에 비추어 농작물 식물에 매우 바람직한 성질이다.

식물의 스트레스 내성 또는 스트레스 허용성으로서 통상적으로 공지된 것을 개선시키는 목적을 성취하려는 다양한 방법들이 개시되었다. 상이한 무생물 스트레스 조건들은 흔히 유해한 반응성 산소 종("ROS"), 예를 들어 초과산화물 또는 과산화 수소를 생성시키므로, 식물에서 스트레스 내성을 개선시키고자 하는 초기의 시도는 상기 ROS 발생의 방지 또는 그의 제거에 초점을 두었다. 이러한 접근법들에 대한 예는 ROS 청소 효소, 예를 들어 카탈라제, 퍼옥시다제, 수퍼옥사이드 디스뮤타제 등의 과발현, 또는 심지어 ROS 청소 분자, 예를 들어 아스코브산, 글루타치 온 등의 양을 증가시키는 것이다. 이러한 접근법 및 스트레스 허용성 식물을 설계하고자 하는 다른 시도들은 문헌[Wang et al. 2003, Planta 218:1-14]에 고찰되어 있다.

식물 세포 및 식물에서 스트레스 허용성은 또한 각각 WO 00/04173 및 PCT/EP 2004/003995에 개시된 바와 같이 내생 폴리-ADP-리보스 폴리머라제(ParP) 또는 폴리(ADP-리보스) 글리코하이드롤라제(ParG)의 활성 또는 수준을 감소시킴으로써 성취될 수 있다. 이러한 식으로, 스트레스 조건이 가해진 식물 세포에서 치명적인 NAD 및 ATP 고갈(외상성 세포 사를 발생시킨다)을 상기 스트레스를 받은 세포에 대해 회피시키거나 충분히 연기시켜 상기 세포를 생존시키고 스트레스 조건에 순응시킬 수 있을 것으로 생각된다.

유키미야(Uchimiya) 등(2002)은 YK1로 표시되는 벼 유전자의 단리뿐만 아니라 도열병 및 다수의 무생물 스트레스, 예를 들어 NaCl, UV-C, 침수 및 과산화 수소에 대한, 키메릭 YK1 유전자를 갖는 트랜스제닉 벼 식물의 허용성을 증가시키는 상기 유전자의 용도를 개시하고 있다(Uchimiya et al., 2002, Molecular breeding 9:25-31).

유키미야 등은 더욱이 벼 세포에서 NAD 의존성 리덕타제 유전자(YK1)의 과발현이 또한 NAD 신시타제 활성을 상향 조절함으로써 NAD(P)(H)의 수준을 촉진시킴을 개시하는 포스터 초록을 공개하였으며, 차례로 이러한 개질이 ROS 스트레스 내성에 필요한 산화환원 물질의 풀을 생성시킨다는 결론을 내렸다(Uchimiya et al. 2003 Keystone symposium on Plant biology: Functions and control of cell death, Snowbird Utah April 10-15, 2003).

효모로부터의 NAD 신시타제가 잘 특성화되었으며 상기는 NAD 신생 합성 경로 및 NAD 회수 경로 모두에서 최종 효소이다(도 1 참조). 상기 신생 경로에서, 퀴놀레이트는 NAD 합성의 전구체이며 트립토판 분해 산물로서 생성된다. 상기 회수 경로에서, 니코틴아미드(NAD의 분해 산물로, 다양한 효소, 예를 들어 PARP, NAD-의존성 데아세틸라제 또는 다른 NAD 글리코하이드롤라제의 작용을 통해 생성된다)는 전구체 분자이다. 첫 번째 단계에서, 니코틴아미드는 니코틴아미다제에 의해 니코틴산으로 탈아미드화된다. 상기 니코틴산은 니코티네이트 포스포리보실 트랜스퍼라제 효소에 의해 5-포스포리보실-1-피로포스페이트로 전달되어 니코틴산 모노뉴클레오타이드를 생성시킨다. 상기 화합물은 상기 신생 경로와 회수 경로 사이에서 공유된다. 따라서, NAD+ 피로포스포릴라제 및 NAD 신시타제에 의한 상기 화합물의 추가의 전환이 상기 신생 경로에서와 같이 성취된다.

효모에서, PNC1(니코틴아미다제를 암호화한다)의 과발현은 칼로리 제한 및 저 강도 스트레스에 의한 수명 연장과 상관되어 왔다(Anderson et al., 2003 Nature 423: p181-185; Gallo et al., 2004, Molecular and Cellular Biology 24:1301-1312).

식물에서 상기 NAD 생합성 경로의 각 효소들에 대해서는 거의 알려져 있지 않다. 헌트(Hunt) 등(2004)은 이들 효소의 식물 상동성을 확인하기 위해 아라비도프시스로부터 입수할 수 있는 게놈 정보를 사용함을 개시한다(Hunt et al., 2004, New Phytologist163(1): 31-44). 동정된 DNA 서열은 하기의 수탁 번호를 갖는다: 니코틴아미다제의 경우: At5g23220; At5g23230 및 At3g16190; 니코티네이트 포스포리보실트랜스퍼라제의 경우: At4g36940, At2g23420, 니코틴산 모노뉴클레오타이드 아데닐트랜스퍼라제의 경우: At5g55810 및 NAD 신시타제의 경우: At1g55090(모든 뉴클레오타이드 서열은 본 발명에 참고로 인용된다).

식물에서 스트레스 허용성을 증가시키기 위한 또 다른 방법들이 여전히 필요하며, 청구의 범위를 포함한, 이후에 개시되는 실시태양들은 그러한 방법 및 수단을 제공한다.

발명의 요약

본 발명의 하나의 실시태양에서, 키메릭 유전자를 식물의 세포에 도입시켜 트랜스제닉 세포를 수득한 다음(이때 상기 키메릭 유전자는 하기의 작동 가능하게 연결된 DNA 단편들, 즉

i. 식물-발현성 프로모터;

ii. 니코틴아미다제, 니코티네이트 포스포리보실트랜스퍼라제, 니코틴산 모노뉴클레오타이드 아데닐 트랜스퍼라제 및 니코틴아미드 아데닌 다이뉴클레오타이드 신시타제 중에서 선택된 니코틴아미드 아데닌 다이뉴클레오타이드 회수 합성 경로의 식물 작용성 효소를 암호화하는 DNA 부위; 및

iii. 전사 종결 및 폴리아데닐화에 수반되는 3' 단부 부위

를 포함한다),

상기 트랜스제닉 세포를 재생시켜 트랜스제닉 식물군을 수득하고; 상기 트랜스제닉 식물군으로부터 증가된 스트레스 내성을 나타내는 식물을 선택하거나, 또는 유사한 비 트랜스제닉 식물에 비해 스트레스 조건 하에서 감소된 반응성 산소 종 수준을 나타내거나 또는 높은 NADH 수준을 유지하는 식물을 선택함을 포함하는, 스트레스 내성이 증가된 식물의 수득 방법을 제공한다. 상기 DNA 부위는 서열식별번호: 2, 서열식별번호: 4, 서열식별번호: 6, 서열식별번호: 8, 서열식별번호: 10, 서열식별번호: 12, 서열식별번호: 14, 서열식별번호: 16, 서열식별번호: 18, 서열식별번호: 20, 서열식별번호: 22, 서열식별번호: 24의 아미노산 서열 중에서 선택된 아미노산 서열을 포함하는 단백질, 또는 서열식별번호: 1, 서열식별번호: 3, 서열식별번호: 5, 서열식별번호: 7, 서열식별번호: 9, 서열식별번호: 11, 서열식별번호: 13, 서열식별번호: 15, 서열식별번호: 17, 서열식별번호: 19, 서열식별번호: 21 또는 서열식별번호: 23의 뉴클레오타이드 서열과 같은 약 60%의 서열 일치성을 갖고 니코틴아미드 아데닌 다이뉴클레오타이드 회수 합성 경로의 효소 활성을 갖는 단백질을 암호화할 수 있다.

또 다른 실시태양에서, 본 발명은 본 발명에 개시된 바와 같은 키메릭 유전자, 상기 키메릭 유전자를 포함하는 식물 세포, 및 상기 키메릭 유전자를 포함하는 식물 세포로 필수적으로 이루어진 식물, 및 상기와 같은 식물의 종자에 관한 것이다. 상기 식물 및 식물 세포는 상기와 같은 키메릭 유전자를 포함하지 않는 유사한 식물보다 스트레스 조건 하에서 더 낮은 반응성 산소 종 수준을 가짐을 특징으로 할 수 있다.

더욱 또 다른 실시태양에서, 본 발명은 식물의 스트레스 내성을 증가시키거나 또는 스트레스 조건 하에서 식물 또는 식물 세포 중의 반응성 산소 종의 수준을 감소시키기 위한 상기 개시된 키메릭 유전자의 용도에 관한 것이다.

본 발명은 더욱이 스트레스 조건 하에서 식물의 스트레스 내성을 증가시키거나 또는 식물 또는 식물 세포 중의 반응성 산소 종의 수준을 감소시키거나 또는 NADH 수준을 유지시키기 위한, 니코틴아미다제, 니코티네이트 포스포리보실트랜스퍼라제, 니코틴산 모노뉴클레오타이드 아데닐 트랜스퍼라제 및 니코틴아미드 아데닌 다이뉴클레오타이드 신시타제 중에서 선택된 니코틴아미드 아데닌 다이뉴클레오타이드 회수 합성 경로의 식물 작용성 효소를 암호화하는 DNA 서열, 예를 들어 서열식별번호: 2, 서열식별번호: 4, 서열식별번호: 6, 서열식별번호: 8, 서열식별번호: 10, 서열식별번호: 12, 서열식별번호: 14, 서열식별번호: 16, 서열식별번호: 18, 서열식별번호: 20, 서열식별번호: 22, 서열식별번호: 24의 아미노산 서열 중에서 선택된 아미노산 서열을 포함하는 단백질, 또는 약 60%의 서열 일치성을 갖고 니코틴아미드 아데닌 다이뉴클레오타이드 회수 합성 경로의 효소 활성을 갖는 단백질을 암호화할 수 있는 DNA 서열, 예를 들어 서열식별번호: 1, 서열식별번호: 3, 서열식별번호: 5, 서열식별번호: 7, 서열식별번호: 9, 서열식별번호: 11, 서열식별번호: 13, 서열식별번호: 15, 서열식별번호: 17, 서열식별번호: 19, 서열식별번호: 21 및 서열식별번호: 23의 뉴클레오타이드 서열 중에서 선택된 뉴클레오타이드 서열을 포함하는 DNA 서열의 용도를 제공한다.

도 1은 빵효모(사카로마이세스 세레비지아(Saccharomyces cerevisea))에서 공지된 바와 같은 NAD 회수 경로 및 신생 NAD 합성 경로의 도식적인 표현이다.

도 2 내지 11은 식물 발현성 조절 요소들의 조절 하에서 NAD 회수 경로 또는 NAD 신생 합성 경로로부터의 효소를 암호화하는 DNA 부위들을 포함하는 다양한 T-DNA 벡터의 도식적인 표현이다. 사용된 약어는 하기와 같다: RB: 오른쪽 T-DNA 경계; 3'35S: CaMV 35S 전사물로부터의 전사 종결 및 폴리아데닐화 신호; Cab22L: Cab22L 전사물의 번역되지 않은 리더 서열; P35S2: CaMV 35S 프로모터; 3'g7: 아그로박테리움 튜메파시엔스 T-DNA 유전자 7로부터의 전사 종결 및 폴리아데닐화 신호; bar: 포스피노트리신 아세틸트랜스퍼라제 암호화 부위; pSSUAra: 아라비도프시스로부터의 루비스코 작은 서브유닛 전사물의 프로모터; LB; 왼쪽 T-DNA 경계; Sm/Sp: 스펙티노마이신 및 스트렙토마이신 내성 유전자; pVS1ori: 아그로박테리움에서의 복제에 적합한 VS1의 기원; ColE1: 복제 기원; NLS: 핵 국소화 신호; PNC1: 사카로마이세스 세레비지아에로부터의 니코틴아미다제를 암호화하는 DNA 부위; npt1: 사카로마이세스 세레비지아에로부터의 니코티네이트 포스포리보실트랜스퍼라제; nma1: 사카로마이세스 세레비지아에로부터의 니코틴산 모노뉴클레오타이드 아데닐 트랜스퍼라제 1; nma2: 사카로마이세스 세레비지아에로부터의 니코틴산 모노뉴클레오타이드 아데닐 트랜스퍼라제 2; qns1: 사카로마이세스 세레비지아로부터의 NAD 신시타제(QNS1).

본 발명은 효모에서 NAD 회수 경로로부터의 식물 작용성 효소를 암호화하는 DNA 서열을 사용하여, 상기 DNA 서열을 포함하지 않는 식물보다 스트레스, 특히 무생물 스트레스에 더 내성인 트랜스제닉 식물을 수득할 수 있다는 발견을 기본으로 한다. 상기 트랜스제닉 식물은 또한 스트레스 조건 하에 두었을 때 대조용 식물에 비해 현저하게 감소된 반응성 산소 종("ROS") 수준을 나타내었으며 높은 수준의 NADH를 유지하였다.

따라서, 본 발명의 하나의 실시태양에서,

-본 발명에 개시된 스트레스 내성 키메릭 유전자를 식물의 세포에 도입시켜 상기 스트레스 내성 키메릭 유전자를 포함하는 세포를 수득하는 단계;

-상기 스트레스 내성 키메릭 유전자를 포함하는 상기 세포를 재생시켜 상기 스트레스 내성 키메릭 유전자를 포함하는 식물군을 수득하는 단계; 및

-상기 식물군으로부터, 스트레스 조건 하에서 유사한 비 트렌스제닉 식물에 비해 증가된 스트레스 내성 및/또는 감소된 ROS 수준을 나타내고/내거나 높은 수준의 NADH를 유지하는 식물을 선택하는 단계

를 포함하는, 증가된 스트레스 내성을 갖는 식물을 수득하는 방법을 제공한다.

상기에 의해 상기 스트레스 내성 키메릭 유전자는 니코틴아미다제, 니코티네이트 포스포리보실트랜스퍼라제, 니코틴산 모노뉴클레오타이드 아데닐 트랜스퍼라제 및 니코틴아미드 아데닌 다이뉴클레오타이드 신시타제 중에서 선택된 니코틴아미드 아데닌 다이뉴클레오타이드 회수 합성 경로의 식물 작용성 효소를 암호화하는 DNA 부위, 및 전사 종결 및 폴리아데닐화에 수반되는 3' 단부 부위에 작동 가능하게 연결된 식물 발현성 프로모터를 포함한다.

본 발명에 사용된 바와 같은 "니코틴아미드 아데닌 다이뉴클레오타이드 회수 합성 경로의 식물 작용성 효소"는 식물에 도입될 때 식물 발현성 프로모터 및 종결자 부위와 같은 적합한 조절 요소에 연결되고, 전사 및 번역되어 식물 세포에서 작용성인 NAD 회수 합성 경로의 효소를 생산할 수 있는 효소이다. 식물 공급원으로부터 수득되는, 상기 NAD 회수 합성으로부터의 효소(및 암호화 유전자)뿐만 아니라 효모(사카로마이세스 세레비지아에) 또는 다른 효모 또는 진균으로부터 수득된 효소가 포함된다. 후자의 단백질이 본 발명에 따른 방법에 훨씬 더 적합할 수 있는데, 그 이유는 상기가 효소 피드백 조절 등을 덜 받을듯하기 때문이다(유사한 식물 유래된 효소들은 상기 조절을 받기 쉬울 수 있다).

상기 NAD 회수 합성 경로에 수반되는 효소는 하기를 포함한다

-니코틴아미드의 아미드 기의 가수분해를 촉진하여 니코티네이트와 NH3을 방출하는 니코틴아미다제(EC 3.5.1.19). 상기 효소는 또한 니코틴아미드 데아미나제, 니코틴아미드 아미다제, YND아제 또는 니코틴아미드 아미도하이드롤라제로서 공지되어 있다.

-나이아신 리보뉴클레오타이다제, 니코틴산 모노뉴클레오타이드 글리코하이드롤라제; 니코틴산 모노뉴클레오타이드 피로포스포릴라제; 니코티네이트-D-리보뉴클레오타이드 + 다이포스페이트 = 니코티네이트 + 5-포스포-α-D-리보스 1-다이포스페이트의 반응을 촉진하는 니코틴산 포스포리보실트랜스퍼라제로서 또한 공지된 니코티네이트 포스포리보실트랜스퍼라제(EC 2.4.2.11);

-데아미도-NAD+ 피로포스포릴라제; 니코티네이트 모노뉴클레오타이드 아데닐일트랜스퍼라제; 데아민도니코틴아미드 아데닌 다이뉴클레오타이드 피로포스포릴라제; NaMT-AT아제; ATP + 니코티네이트 리보뉴클레오타이드 = 다이포스페이트 + 데아미도-NAD⁺의 반응을 촉진하는 니코틴산 모노뉴클레오타이드 아데닐일트랜스퍼라제로서 또한 공지된 니코티네이트-뉴클레오타이드 아데닐일트랜스퍼라제(EC 2.7.7.18);

-NAD 신시타제; NAD⁺ 신타제; 니코틴아미드 아데닌 다이뉴클레오타이드 신시타제; 데아미도-NAD⁺ + ATP + NH3 = AMP + 다이포스페이트 + NAD⁺의 반응을 촉진하는 다이포스포피리딘 뉴클레오타이드 신시타제.

본 발명의 하나의 실시태양에서, 상기 NAD 회수 경로의 상이한 효소들을 암호화하는 암호화 부위는 서열식별번호: 2, 4, 6, 8 또는 10으로 열거되는 아미노산 서열을 갖는 단백질을 암호화하는 뉴클레오타이드 서열, 예를 들어 서열식별번호: 1, 3, 5, 7 또는 9의 뉴클레오타이드 서열을 포함한다.

그러나, 삽입, 결실 및 치환을 포함하여, 상기 뉴클레오타이드 서열의 변체들을 또한 동일한 효과에 사용할 수 있음이 명백할 것이다. 동등하게, 사카로마이세스 세레비지아에와 상이한 종들로부터의 상기 언급한 뉴클레오타이드 서열에 대한 동족체들을 사용할 수 있다. 여기에는 비 제한적으로 식물로부터의 뉴클레오타이드 서열, 및 동일한 아미노산 서열을 갖는 단백질을 암호화하는 뉴클레오타이드 서열뿐만 아니라 상기와 같은 뉴클레오타이드 서열의 변체들이 포함된다. 후자의 예는 서열식별번호: 12, 14, 16, 18, 20, 22 또는 24에 열거된 아미노산 서열을 갖는 단백질을 암호화하는 뉴클레오타이드 서열, 예를 들어 서열식별번호: 11, 13, 15, 17, 19, 21 또는 23의 뉴클레오타이드 서열이다.

상기 개시된 뉴클레오타이드 서열의 변체들은 상기 NAD 회수 경로로부터의 효소를 암호화하는 동정된 뉴클레오타이드 서열, 예를 들어 상기 서열 목록에서 동정된 것들과 약 80% 이상, 또는 85% 또는 90% 또는 95%의 서열 일치성을 가질 것이다. 바람직하게는, 상기 변체들은 상기 NAD 회수 경로로부터의 효소와 동일한 효소 활성을 갖는 작용성 단백질을 암호화할 것이다. 본 발명의 목적을 위해서, 퍼센트로서 표현된, 2 개의 관련된 뉴클레오타이드 또는 아미노산 서열의 "서열 일치성"은 동일한 잔기(x 100)를 비교된 위치의 수로 나눈 2 개의 최적으로 정렬된 서열 중의 위치의 수를 지칭한다. 틈, 즉 잔기가 하나의 서열에는 존재하지만 다른 것에는 존재하지 않는 정렬 중의 위치는 동일하지 않은 잔기를 갖는 위치로 간주한다. 상기 두 서열의 정렬을 니들맨과 분취(Needleman and Wunch 1970) 연산에 의해 수행한다. 상기 컴퓨터 지원된 서열 정렬을 편의상 표준 소프트웨어 프로그램, 예를 들어 50의 틈 생성 패널티 및 3의 틈 연장 패널티를 갖는 디폴트 득점 매트릭스를 사용하는 위스콘신 패키지 버전 10.1(Genetics Computer Group, Madision, Wisconsin, USA)의 일부인 GAP를 사용하여 수행할 수 있다.

효모에서 상기 NAD 회수 경로로부터의 효소를 암호화하거나, 또는 효모와 다른 유기체로부터의 상동 효소를 암호화하는 뉴클레오타이드 서열과 상동성인 뉴클레오타이드 서열을 헌트 등(상기 참조)에 의해 개시된 게놈 데이터의 인실리코 분석에 의해 동정할 수 있다.

상동성인 뉴클레오타이드 서열을 또한 탐침으로서 상기 NAD 회수 경로로부터의 효소를 암호화하는 동정된 뉴클레오타이드 서열, 예를 들어 상기 서열 목록에서 동정된 것들을 사용하여 엄격한 조건 하에서 하이브리드화에 의해 동정 및 단리할 수 있다.

본 발명에 사용된 "엄격한 하이브리드화 조건"은 하이브리드화가 일반적으로 탐침과 표적 서열 간에 95% 이상, 바람직하게는 97% 이상의 서열 일치성이 존재하는 경우 발생함을 의미한다. 엄격한 하이브리드화 조건의 예는 50% 폼아미드, 5 x SSC(150 mM NaCl, 15 mM 시트르산 삼나트륨), 50 mM 인산 나트륨(pH 7.6), 5 x 덴하르트 용액, 10% 덱스트란 설페이트, 및 20 ㎍/㎖의 변성된, 전단된 담체 DNA, 예를 들어 연어 정자 DNA를 포함하는 용액에서 밤새 배양한 다음 상기 하이브리드화 지지체를 대략 65 ℃에서, 바람직하게는 약 10 분간 2 회 0.1 x SSC 중에서 세척하는 것이다. 다른 하이브리드화 및 세척 조건들은 널리 공지되어 있으며 문헌[Sambrook et al., Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor, NY(1989), 특히 11 장]에 예시되어 있다.

상기와 같은 변체 서열을 또한 프라이머로서 상기 NAD 회수 경로로부터의 효소를 암호화하는 유전자에 특이적인 올리고뉴클레오타이드, 예를 들어 비 제한적으로 서열식별번호: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23 또는 이들의 보체의 뉴클레오타이드 서열 중에서 선택된 약 20 내지 약 50 개의 연속적인 뉴클레오타이드를 포함하는 올리고뉴클레오타이드를 사용하여 DNA 증폭에 의해 수득할 수 있다.

본 발명의 방법을 사용하여 상이한 종류의 스트레스 유발 조건, 특히 무생물 스트레스 조건, 예를 들어 침수, 강한 광 조건, 강한 UV 조사 수준, 증가된 과산화 수소 수준, 가뭄 조건, 고온 또는 저온, 증가된 염도 조건에 허용성인 식물을 수득할 수 있다. 본 발명의 방법을 또한 불리한 조건, 특히 무생물 스트레스 조건, 예를 들어 침수, 강한 광 조건, 강한 UV 조사 수준, 증가된 과산화 수소 수준, 가뭄 조건, 고온 또는 저온, 증가된 염도 조건 등의 조건 하에서 생장하는 식물의 세포 중의 ROS의 수준을 감소시키는데 사용할 수 있다. ROS의 수준 또는 NADH의 수준을 당해 분야에 공지된 방법, 예를 들어 실시예 3에 개시된 방법을 사용하여 측정할 수 있다.

본 발명에 개시된 방법을 사용하여, ROS의 수준이 비 스트레스 조건, 예를 들어 비 제한적으로 약한 광 하에서 대조용 식물과 같거나 이보다 낮은 식물을 수득할 수 있다. 이러한 식물에서, 비 스트레스 조건 하에 ROS의 수준은 약한 광 조건 하의 대조용 식물 수준의 50% 내지 100%, 보다 특히 약 60% 내지 약 85%의 범위일 수 있다. 스트레스 조건 하에서 이들 식물 중의 ROS의 수준은 스트레스 조건 하의 대조용 식물에서의 ROS 수준의 약 50% 내지 80%이며, 이는 비 스트레스 조건 하의 대조용 식물에서의 ROS 수준의 약 60 내지 80%에 상응한다. 유사하게, 이들 식물에서 NADH 수준은 비 스트레스 조건, 예를 들어 비 제한적으로 약한 광 하의 대조용 식물과 같거나 이보다 높다. 이러한 식물에서, 비 스트레스 조건 하에서 NADH의 수준은 약한 광 조건 하의 대조용 식물에서의 NADH 수준의 100% 내지 160%, 보다 특히 약 120% 내지 약 140%의 범위일 수 있다. 스트레스 조건 하에서 이들 식물 중의 NADH의 수준은 스트레스 조건 하의 대조용 식물에서의 NADH 수준의 약 200% 내지 300%이며, 이는 비 스트레스 조건 하의 대조용 식물에서의 ROS 수준의 약 100 내지 160%에 상응한다.

트랜스제닉 식물을 수득하기 위한 방법은 본 발명에서 중요하지 않은 것으로 생각되며 특정한 식물 종에 적합한 임의의 형질전환 방법 및 재생을 사용할 수 있다. 상기와 같은 방법은 당해 분야에 널리 공지되어 있으며 아그로박테리움(Agrobacterium)-매개된 형질전환, 입자 건 전달, 미세주입, 완전 세포의 전기천공, 폴리에틸렌글리콜-매개된 원형질체 형질전환, 원형질체의 전기천공, 리포솜 매개된 형질전환, 규소-휘스커 매개된 형질전환 등이 있다. 이어서 이러한 방식으로 수득된 형질전환된 세포를 성숙한 번식력 있는 식물로 재생시킬 수 있다.

상기 수득된 형질전환된 식물을 통상적인 번식 계획에 사용하여 동일한 특성을 갖는 보다 많은 형질전환된 식물을 생산하거나, 또는 동일하거나 관련된 식물 종의 다른 품종에 또는 하이브리드 식물에 본 발명에 따른 키메릭 유전자를 도입시킬 수 있다. 상기 형질전환된 식물로부터 수득된 종자는 안정한 게놈 삽입물로서 본 발명의 키메릭 유전자를 함유하며 이는 또한 본 발명에 포함된다.

상기 NAD 회수 경로로부터의 상이한 효소들을 암호화하는 DNA 부위를 갖는, 본 발명에 개시된 상이한 스트레스 내성 키메릭 유전자들을 하나의 식물 세포 또는 식물 내에서 결합시켜 상기 키메릭 유전자를 포함하는 식물의 스트레스 허용성을 더욱 향상시킬 수 있음은 분명할 것이다. 따라서, 본 발명의 하나의 실시태양에서, 각각 상이한 암호화 부위를 포함하는 2 개 이상의 스트레스 내성 키메릭 유전자를 포함하는 식물 세포 및 식물을 제공한다.

본 발명에 따른 트랜스제닉 식물 세포 및 식물 주는 WO 00/04173 및 PCT/EP2004/003995에 개시된 바와 같은 내생 PARP 및/또는 PARG 유전자의 발현을 감소시키는 키메릭 유전자를 추가로 포함할 수 있다.

이러한 추가의 키메릭 유전자를, 예를 들어 본 발명의 트랜스제닉 식물 주를 PARP 및/또는 PARG 유전자 발현 감소 키메릭 유전자를 함유하는 트랜스제닉 식물과 교배시킴으로써 도입시킬 수 있다. 트랜스제닉 식물 세포 또는 식물 주를 또한 본 발명의 키메릭 유전자를 PARP 또는 PARG 유전자 발현 감소 키메릭 유전자를 포함하는 트랜스제닉 식물 세포 내로 또는 이와 역으로 도입 또는 형질전환시킴으로써 수득할 수 있다.

본 발명의 목적을 위해서, 프로모터는 식물 발현성 프로모터이다. 본 발명에 사용된 "식물 발현성 프로모터"란 용어는 식물 세포에서 전사를 조절(개시)할 수 있는 DNA 서열을 의미한다. 상기는 식물 기원의 임의의 프로모터뿐만 아니라 식물 세포에서 전사를 조정할 수 있는 비 식물 기원의 임의의 프로모터, 즉 바이러스 또는 세균 기원의 몇몇 프로모터, 예를 들어 CaMV35S(Harpster et al., 1988 Mol. Gen. Genet. 212, 182-190), 지하 클로버 바이러스 프로모터 No 4 또는 No 7(WO9606932), 또는 T-DNA 유전자 프로모터뿐만 아니라 조직 특이성 또는 기관 특이성 프로모터, 예를 들어 비 제한적으로 종자 특이성 프로모터(예를 들어 WO 89/03887), 기관 초생의 특이성 프로모터(An et al., 1996, The Plant Cell 8, 15-30), 줄기 특이성 프로모터(Keller et al., 1988, EMBO J. 7, 3625-3633), 잎 특이성 프로모터(Hudspeth et al., 1989, Plant Mol Biol 12, 579-589), 잎살 특이성 프로모터(예를 들어 광 유도성 루비스코 프로모터), 뿌리 특이성 프로모터(Keller et al., 1989, Genes Devel. 3, 1639-1646), 괴경 특이성 프로모터(Keil et al., 1989, EMBO J. 8, 1323-1330), 맥관 조직 특이성 프로모터(Peleman et al., 1989, Gene 84, 359-369), 수술 선택성 프로모터(WO 89/10396, WO 92/13956), 열개 대역 특이성 프로모터(WO 97/13865) 등을 포함한다.

본 발명의 키메릭 유전자는 또한 SV40 NLS와 같이 상기 NAD 회수 경로의 효소를 암호화하는 DNA 부위에 작동 가능하게 연결된, 식물에서 작용성인 핵 국소화 신호("NLS")를 갖출 수 있다.

본 문헌을 읽으면, 당해 분야의 숙련가는 NAD 회수 경로 효소들을 암호화하는 내생 유전자가 보다 활성이거나 높은 수준으로 발현되는 식물의 천연 변체들이수득될 때마다 증가된 스트레스 내성에 관한 유사한 효과들을 획득할 수 있음을 바로 이해할 것이다. 상기와 같은 변형 식물들은 식물군을 돌연변이, 예를 들어 비 제한적으로 EMS 돌연변이시킨 다음 상기 NAD 회수 경로 효소들 중 임의의 하나 또는 이들의 조합의 증가된 활성을 선별함으로써 수득될 수 있다.

상이한 변종 또는 품종 군을 일반적으로 또는 특별하게 선택된 무생물 스트레스의 상기 언급한 스트레스 조건에 대한 증가된 허용성에 대해서 선별하고, 이어서 상기 스트레스 조건에 대해 증가된 허용성과 상기 NAD 회수 경로 효소의 효소를 암호화하는 내생 유전자들 중 임의의 유전자의 특정한 대립유전자의 존재와의 상관성에 대해 선별할 수 있음이 또한 바로 명백해질 것이다. 이어서 상기와 같은 대립유전자를 상기 종들이 성적으로 양립성인 경우 교배에 의해 관심 식물에 도입시키거나, 또는 상기를 본 발명에 개시된 통상적인 기법(하이브리드화 또는 PCR 증폭 포함)을 사용하여 동정하고 재조합 DNA 기법을 사용하여 도입시킬 수 있다. 번식 기법을 사용하여 특별히 목적하는 대립유전자를 도입시킨 다음 관심 대립유전자에 특이적인 분자 마커를 사용할 수 있다.

본 발명에 개시된 방법 및 수단은 모든 식물 세포 및 식물, 쌍떡잎 및 외떡입 식물 세포 모두, 및 예를 들어 비 제한적으로 목화, 배추과 식물, 평지 유지종자, 밀, 옥수수, 보리, 해바라기, 벼, 귀리, 사탕수수, 대두, 야채(치커리, 상추, 토마토 포함), 담배, 감자, 사탕무, 파파야, 파인애플, 망고, 아라비도프시스 탈리아나뿐만 아니라 원예, 화훼 또는 삼림에 사용되는 식물에 적합한 것으로 여겨진다.

본 발명에 사용된 "포함하는"은 서술된 특징, 정수, 단계 또는 언급되는 성분들의 존재를 명시하는 것으로 이해되어야 하지만, 하나 이상의 특징, 정수, 단계 또는 성분 또는 이들의 그룹의 존재 또는 첨가를 제외하는 것은 아니다. 따라서, 예를 들어 뉴클레오타이드 또는 아미노산의 서열을 포함하는 핵산 또는 단백질은 실제로 인용된 것들보다 많은 뉴클레오타이드 또는 아미노산을 포함할 수 있다, 즉 보다 큰 핵산 또는 단백질 중에 묻혀있을 수 있다. 작용적으로 또는 구조적으로 한정된 DNA 부위를 포함하는 키메릭 유전자는 추가의 DNA 부위 등을 포함할 수 있다.

하기의 비 제한적인 실시예들은 식물 세포 및 식물에서 스트레스 내성을 증가시키는 키메릭 유전자의 구조 및 상기와 같은 유전자의 용도를 개시한다.

실시예에 달리 나타내지 않는 한, 모든 재조합 DNA 기법들을 문헌[Sambrook et al.(1989) Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press, NY and in Volumes 1 and 2 of Ausubel et al.(1994) Current Protocols in Molecular Biology, Current Protocols, USA]에 개시된 바와 같은 표준 프로토콜에 따라 수행한다. 식물 분자 연구에 대한 표준 물질 및 방법은 BIOS 사이언티픽 퍼블리케이션스 리미티드(Scientific Publications Ltd, UK)와 블랙웰(Blackwell) 사이언티픽 퍼블리케이션스(UK)가 공동으로 발행한 문헌[R.D.D. Croy, Plant Molecular Biology Labfax(1993)]에 개시되어 있다. 표준 분자 생물 기법에 대한 다른 참고문헌들로는 문헌[Sambrook and Russell(2001) Molecular Cloning: A Laboratory Manual, Third Edition, Cold Spring Harbor Laboratory Press, NY, Volumes I and II of Brown(1998) Molecular Biology LabFax, Second Edition, Academic Press(UK)]이 있다. 폴리머라제 쇄 반응에 대한 표준 물질 및 방법을 문헌[Dieffenbach and Dveksler(1995) PCR Primer: A Laboratory Manual, Cold Spring Harbor Laboratory Press, and McPherson et al.(2000) PCR-Basics: From Background to Bench, First Edition, Springer Verlag, Germany]에서 찾을 수 있다.

상기 설명 및 실시예 전체를 통해 하기의 서열들을 참고로 한다:

서열식별번호: 1: 사카로마이세스 세레비지아에로부터의 니코틴아미다제의 뉴클레오타이드 서열(PNC1).

서열식별번호: 2: 사카로마이세스 세레비지아에로부터의 니코틴아미다제의 아미노산 서열(PNC1).

서열식별번호: 3: 사카로마이세스 세레비지아에로부터의 니코티네이트 포스포리보실트랜스퍼라제의 뉴클레오타이드 서열(NPT1)(보체).

서열식별번호: 4: 사카로마이세스 세레비지아에로부터의 니코티네이트 포스포리보실트랜스퍼라제의 아미노산 서열(NPT1).

서열식별번호: 5: 사카로마이세스 세레비지아에로부터의 니코틴산 모노뉴클레오타이드 아데닐 트랜스퍼라제 1의 뉴클레오타이드 서열(NMA1).

서열식별번호: 6: 사카로마이세스 세레비지아에로부터의 니코틴산 모노뉴클레오타이드 아데닐 트랜스퍼라제 1의 아미노산 서열(NMA1).

서열식별번호: 7: 사카로마이세스 세레비지아에로부터의 니코틴산 모노뉴클레오타이드 아데닐 트랜스퍼라제 2의 뉴클레오타이드 서열(NMA2).

서열식별번호: 8: 사카로마이세스 세레비지아에로부터의 니코틴산 모노뉴클레오타이드 아데닐 트랜스퍼라제 2의 아미노산 서열(NMA2).

서열식별번호: 9: 사카로마이세스 세레비지아에로부터의 NAD 신시타제의 뉴클레오타이드 서열(QNS1).

서열식별번호: 10: 사카로마이세스 세레비지아에로부터의 NAD 신시타제의 아미노산 서열(QNS1).

서열식별번호: 11: 아라비도프시스 탈리아나(Arabidopsis thaliana)로부터의 니코틴아미다제의 뉴클레오타이드 서열(동형 1).

서열식별번호: 12: 아라비도프시스 탈리아나로부터의 니코틴아미다제의 아미노산 서열(동형 1).

서열식별번호: 13: 아라비도프시스 탈리아나로부터의 니코틴아미다제의 뉴클레오타이드 서열(동형 2).

서열식별번호: 14: 아라비도프시스 탈리아나로부터의 니코틴아미다제의 아미노산 서열(동형 2).

서열식별번호: 15: 아라비도프시스 탈리아나로부터의 니코틴아미다제의 뉴클레오타이드 서열(동형 3).

서열식별번호: 16: 아라비도프시스 탈리아나로부터의 니코틴아미다제의 아미노산 서열(동형 3).

서열식별번호: 17: 아라비도프시스 탈리아나로부터의 니코티네이트 포스포리보실트랜스퍼라제의 뉴클레오타이드 서열(동형 1).

서열식별번호: 18: 아라비도프시스 탈리아나로부터의 니코티네이트 포스포리보실트랜스퍼라제의 아미노산 서열(동형 1).

서열식별번호: 19: 아라비도프시스 탈리아나로부터의 니코티네이트 포스포리보실트랜스퍼라제의 뉴클레오타이드 서열(동형 2).

서열식별번호: 20: 아라비도프시스 탈리아나로부터의 니코티네이트 포스포리보실트랜스퍼라제의 아미노산 서열(동형 2).

서열식별번호: 21: 아라비도프시스 탈리아나로부터의 니코틴산 모노뉴클레오타이드 아데닐 트랜스퍼라제의 뉴클레오타이드 서열.

서열식별번호: 22: 아라비도프시스 탈리아나로부터의 니코틴산 모노뉴클레오타이드 아데닐 트랜스퍼라제의 아미노산 서열.

서열식별번호: 23: 아라비도프시스 탈리아나로부터의 NAD 신시타제의 뉴클레오타이드 서열.

서열식별번호: 24: 아라비도프시스 탈리아나로부터의 NAD 신시타제의 아미노산 서열.

서열식별번호: 25: T-DNA 벡터 pTVE 467의 뉴클레오타이드 서열

서열식별번호: 26: T-DNA 벡터 pTVE 468의 뉴클레오타이드 서열

서열식별번호: 27: T-DNA 벡터 pTVE 469의 뉴클레오타이드 서열

서열식별번호: 28: T-DNA 벡터 pTVE 470의 뉴클레오타이드 서열

서열식별번호: 29: T-DNA 벡터 pTVE 496의 뉴클레오타이드 서열

서열식별번호: 30: T-DNA 벡터 pTVE 497의 뉴클레오타이드 서열

서열식별번호: 31: T-DNA 벡터 pTVE 500의 뉴클레오타이드 서열

서열식별번호: 32: T-DNA 벡터 pTVE 501의 뉴클레오타이드 서열

서열식별번호: 33: T-DNA 벡터 pTVE 502의 뉴클레오타이드 서열

서열식별번호: 34: T-DNA 벡터 pTVE 503의 뉴클레오타이드 서열

실시예 1: 스트레스 내성 키메릭 유전자의 조립 및 식물 내로의 도입

pTVE467

식물에서 스트레스 내성을 증가시키기 위해서, 통상적인 기법을 사용하여 하기의 DNA 단편을 순서대로 포함하는 키메릭 유전자를 제작하였다:

·콜리플라워 모자이크 바이러스(CaMV 35S)로부터의 프로모터 부위;

·번역되지 않은 리더 Cab22L에 상응하는 약 60 bp의 DNA 단편;

·사카로마이세스 세레비지아에로부터의 니코틴아미다제를 암호화하는 DNA 단편(서열식별번호 1);

·CaMV의 35S 전사물(3'35S)로부터의 3' 번역되지 않은 단부의 단편.

상기 키메릭 유전자를 제초제 포스피노트리신에 내성을 제공하는 선택성 마커 유전자와 함께 T-DNA로부터의 왼쪽과 오른쪽 경계 서열 사이에서, 상기 T-DNA 벡터에 도입시켜 pTVE467(서열식별번호 25)을 생성시켰다. T-DNA 벡터 pTVE467을 도 2에 도식적으로 나타낸다.

T-DNA 벡터 pTVE467은 하기의 분자 특성들을 포함한다:

(C)는 상보적인 가닥을 가리킨다.

pTVE468

pTVE467 중에 존재하는 바와 유사한 키메릭 유전자(여기에서 니코틴아미다제에는 통상적인 핵 국소화 신호가 갖추어져 있다)를 제작하였다. 따라서, 상기 키메릭 유전자는 하기의 작동 가능하게 연결된 DNA 단편들을 포함한다:

·번역되지 않은 리더 Cab22L에 상응하는 약 60 nt의 DNA 단편;

·핵 국소화 신호(NLS)를 포함하는 펩타이드를 암호화하는 약 20 nt의 DNA 단편;

·사카로마이세스 세레비지아에로부터의 니코틴아미다제를 암호화하는 DNA 단편(서열식별번호 1); 이에 의해 상기 NLS 신호가 프레임 안에 융합된다;

상기 키메릭 유전자를 제초제 포스피노트리신에 내성을 제공하는 선택성 마커 유전자와 함께 T-DNA로부터의 왼쪽과 오른쪽 경계 서열 사이에서, 상기 T-DNA 벡터에 도입시켜 pTVE468(서열식별번호 26)을 생성시켰다. T-DNA 벡터 pTVE468을 도 3에 도식적으로 나타낸다.

T-DNA 벡터 pTVE468은 하기의 분자 특성들을 포함한다:

(C)는 상보적인 가닥을 가리킨다.

pTVE469

·번역되지 않은 리더 Cab22L에 상응하는 약 60 bp의 DNA 단편;

·사카로마이세스 세레비지아에로부터의 니코티네이트 포스포리보실트랜스퍼라제를 암호화하는 DNA 단편(NPT1; 서열식별번호 3);

상기 키메릭 유전자를 제초제 포스피노트리신에 내성을 제공하는 선택성 마커 유전자와 함께 T-DNA로부터의 왼쪽과 오른쪽 경계 서열 사이에서, 상기 T-DNA 벡터에 도입시켜 pTVE469(서열식별번호 27)를 생성시켰다. T-DNA 벡터 pTVE469를 도 4에 도식적으로 나타낸다.

T-DNA 벡터 pTVE469는 하기의 분자 특성들을 포함한다:

(C)는 상보적인 가닥을 가리킨다.

pTVE470

pTVE469 중에 존재하는 바와 유사한 키메릭 유전자(여기에서 사카로마이세스 세레비지아에로부터의 니코티네이트 포스포리보실트랜스퍼라제에는 통상적인 핵 국소화 신호가 갖추어져 있다)를 제작하였다. 따라서, 상기 키메릭 유전자는 하기의 작동 가능하게 연결된 DNA 단편들을 포함한다:

·번역되지 않은 리더 Cab22L에 상응하는 약 60 nt의 DNA 단편;

·사카로마이세스 세레비지아에로부터의 니코티네이트 포스포리보실트랜스퍼라제를 암호화하는 DNA 단편(NPT1; 서열식별번호 3); 이에 의해 상기 NLS 신호가 프레임 안에 융합된다;

상기 키메릭 유전자를 제초제 포스피노트리신에 내성을 제공하는 선택성 마커 유전자와 함께 T-DNA로부터의 왼쪽과 오른쪽 경계 서열 사이에서, 상기 T-DNA 벡터에 도입시켜 pTVE470(서열식별번호 28)을 생성시켰다. T-DNA 벡터 pTVE470을 도 5에 도식적으로 나타낸다.

T-DNA 벡터 pTVE470은 하기의 분자 특성들을 포함한다:

(C)는 상보적인 가닥을 가리킨다.

pTVE496

·번역되지 않은 리더 Cab22L에 상응하는 약 60 bp의 DNA 단편;

·사카로마이세스 세레비지아에로부터의 니코틴산 모노뉴클레오타이드 아데닐 트랜스퍼라제 1을 암호화하는 DNA 단편(NMA1; 서열식별번호 5);

상기 키메릭 유전자를 제초제 포스피노트리신에 내성을 제공하는 선택성 마 커 유전자와 함께 T-DNA로부터의 왼쪽과 오른쪽 경계 서열 사이에서, 상기 T-DNA 벡터에 도입시켜 pTVE496(서열식별번호 29)을 생성시켰다. T-DNA 벡터 pTVE496을 도 6에 도식적으로 나타낸다.

T-DNA 벡터 pTVE496은 하기의 분자 특성들을 포함한다:

(C)는 상보적인 가닥을 가리킨다.

pTVE497

pTVE496 중에 존재하는 바와 유사한 키메릭 유전자(여기에서 사카로마이세스 세레비지아에로부터의 니코틴산 모노뉴클레오타이드 아데닐 트랜스퍼라제 1에는 통상적인 핵 국소화 신호가 갖추어져 있다)를 제작하였다. 따라서, 상기 키메릭 유전자는 하기의 작동 가능하게 연결된 DNA 단편들을 포함한다:

·번역되지 않은 리더 Cab22L에 상응하는 약 60 nt의 DNA 단편;

·사카로마이세스 세레비지아에로부터의 니코틴산 모노뉴클레오타이드 아데 닐 트랜스퍼라제 1을 암호화하는 DNA 단편(NMA1; 서열식별번호 5); 이에 의해 상기 NLS 신호가 프레임 안에 융합된다;

상기 키메릭 유전자를 제초제 포스피노트리신에 내성을 제공하는 선택성 마커 유전자와 함께 T-DNA로부터의 왼쪽과 오른쪽 경계 서열 사이에서, 상기 T-DNA 벡터에 도입시켜 pTVE497(서열식별번호 30)을 생성시켰다. T-DNA 벡터 pTVE497을 도 7에 도식적으로 나타낸다.

T-DNA 벡터 pTVE497은 하기의 분자 특성들을 포함한다:

(C)는 상보적인 가닥을 가리킨다.

pTVE500

·번역되지 않은 리더 Cab22L에 상응하는 약 60 bp의 DNA 단편;

·사카로마이세스 세레비지아에로부터의 니코틴산 모노뉴클레오타이드 아데 닐 트랜스퍼라제 2를 암호화하는 DNA 단편(NMA2; 서열식별번호 7);

상기 키메릭 유전자를 제초제 포스피노트리신에 내성을 제공하는 선택성 마커 유전자와 함께 T-DNA로부터의 왼쪽과 오른쪽 경계 서열 사이에서, 상기 T-DNA 벡터에 도입시켜 pTVE500(서열식별번호 31)을 생성시켰다. T-DNA 벡터 pTVE500을 도 8에 도식적으로 나타낸다.

T-DNA 벡터 pTVE500은 하기의 분자 특성들을 포함한다:

(C)는 상보적인 가닥을 가리킨다.

pTVE501

pTVE500 중에 존재하는 바와 유사한 키메릭 유전자(여기에서 사카로마이세스 세레비지아에로부터의 니코틴산 모노뉴클레오타이드 아데닐 트랜스퍼라제 2에는 통상적인 핵 국소화 신호가 갖추어져 있다)를 제작하였다. 따라서, 상기 키메릭 유전자는 하기의 작동 가능하게 연결된 DNA 단편들을 포함한다:

·번역되지 않은 리더 Cab22L에 상응하는 약 60 nt의 DNA 단편;

·사카로마이세스 세레비지아에로부터의 니코틴산 모노뉴클레오타이드 아데닐 트랜스퍼라제 2를 암호화하는 DNA 단편(NMA2; 서열식별번호 7); 이에 의해 상기 NLS 신호가 프레임 안에 융합된다;

상기 키메릭 유전자를 제초제 포스피노트리신에 내성을 제공하는 선택성 마커 유전자와 함께 T-DNA로부터의 왼쪽과 오른쪽 경계 서열 사이에서, 상기 T-DNA 벡터에 도입시켜 pTVE502(서열식별번호 32)를 생성시켰다. T-DNA 벡터 pTVE501을 도 9에 도식적으로 나타낸다.

T-DNA 벡터 pTVE501은 하기의 분자 특성들을 포함한다:

(C)는 상보적인 가닥을 가리킨다.

pTVE502

·번역되지 않은 리더 Cab22L에 상응하는 약 60 bp의 DNA 단편;

·사카로마이세스 세레비지아에로부터의 NAD 신타제를 암호화하는 DNA 단편(QNS1; 서열식별번호 9);

상기 키메릭 유전자를 제초제 포스피노트리신에 내성을 제공하는 선택성 마커 유전자와 함께 T-DNA로부터의 왼쪽과 오른쪽 경계 서열 사이에서, 상기 T-DNA 벡터에 도입시켜 pTVE502(서열식별번호 33)를 생성시켰다. T-DNA 벡터 pTVE502를 도 10에 도식적으로 나타낸다.

T-DNA 벡터 pTVE502는 하기의 분자 특성들을 포함한다:

(C)는 상보적인 가닥을 가리킨다.

pTVE503

pTVE502 중에 존재하는 바와 유사한 키메릭 유전자(여기에서 사카로마이세스 세레비지아에로부터의 NAD 신타제에는 통상적인 핵 국소화 신호가 갖추어져 있다)를 제작하였다. 따라서, 상기 키메릭 유전자는 하기의 작동 가능하게 연결된 DNA 단편들을 포함한다:

·번역되지 않은 리더 Cab22L에 상응하는 약 60 nt의 DNA 단편;

·사카로마이세스 세레비지아에로부터의 NAD 신타제를 암호화하는 DNA 단편(QNS1; 서열식별번호 ); 이에 의해 상기 NLS 신호가 프레임 안에 융합된다;

상기 키메릭 유전자를 제초제 포스피노트리신에 내성을 제공하는 선택성 마커 유전자와 함께 T-DNA로부터의 왼쪽과 오른쪽 경계 서열 사이에서, 상기 T-DNA 벡터에 도입시켜 pTVE503(서열식별번호 34)을 생성시켰다. T-DNA 벡터 pTVE503을 도 11에 도식적으로 나타낸다.

T-DNA 벡터 pTVE503은 하기의 분자 특성들을 포함한다:

(C)는 상보적인 가닥을 가리킨다.

통상적인 방법을 사용하여 상기 T-DNA 벡터들을 헬퍼 Ti-플라스미드를 포함하는 아그로박테리움 균주 내에 도입시켰다. 상기 키메릭 유전자를 당해 분야에 개시된 바와 같이 아그로박테리움 매개된 형질전환에 의해 아라비도프시스 식물 내에 도입시켰다.

실시예 2. 실시예 1에 개시된 키메릭 유전자들을 포함하는 트랜스제닉 아라비도프 시스 주의 분석

실시예 1에 개시된 바와 같이 수득된, NAD-회수 경로의 효모 유전자를 발현하는 트랜스제닉 아라비도프시스 주(T1-세대)의 종자를 발아시키고 15 ㎎ L^-1의 포스피노트리신(PPT)을 함유하는 배지 상에서 생장시켰다. 아라비도프시스 탈리아나 cv Col-0을 대조군으로서 사용하였다.

모든 식물들에 강한 광 스트레스를 가하였다. 30 μ아인슈타인 m^-2초^-1에서 생장시킨 2 주된 식물을 6 시간 동안 250 μ아인슈타인 m^-2초^-1(강한 광)로 이동시킨 다음, 암실에서 8 시간 및 다시 강한 빛에서 8 시간으로 이동시켰다.

상기 처리 후에, NADH 함량 및 초과산화물 라디칼 함량을 모든 주들에 대해 측정하고 약한 광 조건 하에서 생장시킨 트랜스제닉 및 대조용 주들에서의 동일한 화합물의 측정과 비교하였다. 결과를 표 1에 요약한다.

트랜스제닉 식물은 강한 광 하에서 대조용 식물보다 더 높은 NADH 함량을 나타내었으며, 강한 광 하에서 대조용 식물보다 적은 반응성 산소 종을 생성시켰다. 암호화된 NAD 회수 경로 효소에 핵 국소화 신호가 갖추어졌거나 그렇지 못한 구조물들간의 차이는 관찰되지 않았다.

트랜스제닉 식물 주들을 또한 강한 광 스트레스 조건에 대한 허용성에 대해 표현형에 의해 등급화하였다. 이를 위해, 식물들을 2 주간 약한 광 조건(30 μ아인슈타인 m^-2초^-1) 하에 생체 외에서 생장시키고 3 일간 강한 광 조건(250 μ아인슈타인 m^-2초^-1; 16 시간 명 - 8시간 암)으로 이동시켰다. 상기 강한 광 처리 후에, 상기 식물들을 약한 광 조건으로 복귀시키고 표현형 기록 전에 추가로 3 일간 생장시켰다.

대조용 식물은 작았으며 개화가 시작(스트레스 유발됨)된 반면, 실시예 1에 개시된 바와 같은 키메릭 유전자를 포함하는 트랜스제닉 주의 식물들은 대조용 식물보다 더 컸고 단지 웃자라기 시작하였다.

실시예 3: NADH 함량 및 초과산화물 함량의 측정을 위한 프로토콜

수용성 테트라졸륨 염을 사용한 세포 내 NAD (P)H 정량분석

참고문헌

[Jun Nakamura, Shoji Asakura, Susan D. Hester, Gilbert de Murcia, Keith W. Caldecott and James A. Swenberg(2003) 세포 내 NAD(P)H의 정량 분석은 염기 삭제 복원 결함 세포에서 DNA 단일 가닥 파괴 복원의 불균형을 실시간 모니터할 수 있다. Nucleic Acids Research 31(17), e104.]

식물 물질

대부분의 식물 물질을 사용할 수 있다:

-생체 외 생장된 아라비도프시스 새싹 14 내지 18일 된 것, 그러나 개화하지는 않음

-평지 유지종자의 배축 외식편

세포 계수 키트 -8( CCK -8)

Sopachem n.v./Belgium

72A, Avenue du Laarbeeklaan - 1090 Brussels

Belgium

내용물:

5 m몰/L WST-8(테트라졸륨 염), 0.2 m몰/L 1-메톡시 PMS, 150 m몰/L NaCl을 함유하는 5 ㎖ 병

반응 용액:

-25 mM K-포스페이트 완충액(pH 7.4) 10 ㎖

-CCK-8 0.5 ㎖

-0.1 mM 1-메톡시-5-메틸펜아지늄 메틸 설페이트(=1-메톡시펜아진 메토설페이트): 1 ㎕/㎖의 100 mM 모액(MW = 336.4; 물 2.973 ㎖ 중의 100 ㎎)

-1 방울의 트윈20/25 ㎖

과정

-식물 물질을 수확하고 25 mM K-포스페이트 완충액(pH 7.4) 중에 넣는다

예를 들어: 150 평지 유지종자의 배축 외식편

1 gr 아라비도프시스 새싹(뿌리 없음)

-완충액을 반응 용액으로 대체한다

1 gr의 아라비도프시스 새싹에 대해 15 ㎖

150 평지 유지종자의 배축 외식편에 대해 15 ㎖

-암실에서 26 ℃에서 약 1/2 시간 배양한다(반응에 이어서)

-450 ㎚에서 반응 용액의 흡광도를 측정한다.

XTT 의 환원을 정량분석함으로써 초과산화물 생성을 측정한다

참고문헌[De Block, M., De Brouwer, D.(2002) 평지 유지종자 주 및 하이브리드의 활력을 정량분석하기 위한 간단하고 확실한 생체 외 분석. Plant Physiol. Biochem. 40, 845-852]

A. 브라시카 나푸스

배지 및 반응 완충액

파종 배지(배지 201):

절반 농축된 무라쉬게와 스쿠그 염

2% 슈크로스

pH 5.8

0.6% 아가(Difco Bacto Agar)

250 ㎎/ℓ 트라이아실린

캘러스 유도 배지 A2S3:

MS 배지, 0.5 g/ℓ Mes(pH 5.8), 3% 슈크로스, 40 ㎎/ℓ 아데닌-SO₄, 0.5% 아가로스, 1 ㎎/ℓ 2,4-D, 0.25 ㎎/ℓ NAA, 1 ㎎/ℓ BAP, 250 ㎎/ℓ 트라이아실린

반응 완충액:

25 mM K-포스페이트 완충액 pH 8

1 mM 나트륨, 3'-{1-[페닐아미노-카보닐]-3,4-테트라졸륨}-비스(4-메톡시-6-나이트로) = XTT(BioVectra, Canada)(MW 674.53)

XTT를 조심스럽게 가온한 용액(±37 ℃)으로 용해시킨다(사용 전에 실온으로 냉각시킨다)

완충액 25 ㎖에 대해 1 방울의 트윈20

종자의 살균-종자의 사전 발아-묘목의 생장

종자를 2 분간 70% 에탄올에 담그고, 이어서 15 분간 0.1% 트윈20을 함유하는 차아염소산 나트륨 용액(약 6% 활성 염소 함유) 중에 담근다. 최종적으로, 상기 종자를 1 ℓ의 멸균 수로 세정한다.

종자를 멸균 수중에서 1 시간 이상 배양한다(발아를 억제할 수 있는 성분들의 종자로부터의 확산을 허용한다).

종자를 50 ㎖의 멸균수(+250 ㎎/ℓ 트라이아실린)를 함유하는 250 ㎖ 삼각 플라스크에 넣는다. 약 20 시간 동안 진탕시킨다.

유근이 돌출된 종자를 약 125 ㎖의 파종 배지(10 종자/용기, 오염에 의한 종자 손실을 감소시키기 위해 너무 많지 않아야 한다)를 함유하는 듀체파(Duchefa)의 비트로 벤트(Vitro Vent) 용기에 넣는다. 상기 종자를 16 시간의 광주기로 ±24 ℃ 및 10 내지 30 μ 아인슈타인 s^-1m^-1에서 발아시킨다.

단서: 파종해야할 종자 량의 계산: 5 배축 조각/묘목

상기 배축 외식편의 사전 배양 및 스트레스의 유발

-파종 후 12 내지 14일째에, 상기 배축을 약 7 내지 10 ㎜ 조각으로 절단한다.

-상기 배축 외식편(25 배축/Optilux Petridish, Falcon S1005, Denmark)을 25 ℃(10 내지 30 μ 아인슈타인 s^-1m^-1)에서 배지 A2S3 상에서 5일간 배양한다.

단서: 조건당 150 개의 배축 외식편을 사용한다.

-스트레스의 유발:

배축 외식편을 각각 0, 25 및 50 ㎎/ℓ의 아세틸살리실산을 함유하는 A2S3 배지로 옮긴다.

16 시간의 광 주기로 25 ℃ 및 10 내지 30 μ 아인슈타인 s^-1m^-1에서 약 24 시간 동안 배양한다.

XTT -분석

-150 개 배축 외식편을 50 ㎖ 팔콘 튜브로 옮긴다.

-반응 완충액(XTT 없음)으로 세척한다.

-반응 완충액 + XTT 20 ㎖을 가한다(외식편이 잠겨야 하지만 진공 침투되지는 않는다).

-26 ℃에서 암실에서 배양한다.

-상기 반응에 이어서 470 ㎚에서 반응 배지의 흡수를 측정한다.

B. 아라비도프시스 탈리아나

배지 및 반응 완충액

식물 배지:

절반 농축된 무라쉬게와 스쿠그 염

B5 비타민

1.5% 슈크로스

pH 5.8

0.7% 디프코 아가

배양 배지:

1/2 농축된 MS-염

1% 슈크로스,

0.5 g/ℓ MES pH 5.8

25 ㎖ 배지에 대해 1 방울의 트윈 20

반응 완충액:

25 mM K-포스페이트 완충액 pH 8

완충액 25 ㎖에 대해 1 방울의 트윈20

아라비도프시스 식물

-아라비도프시스 주: 시험하기 위한 대조용 주(시험된 주들이 유래된 모 주)

-아라비도프시스 종자의 살균:

2 분 70% 에탄올

10 분 표백(6% 활성 염소) + 20 ㎖의 용액에 대해 1 방울의 트윈 20

멸균 수로 5 회 세척

단서: 살균을 2 ㎖의 에펜도르프 튜브에서 수행한다. 아라비도프시스 종자는 상기 튜브의 바닥에 가라앉으며 1 ㎖ 피펫맨에 의해 상기 액체를 제거한다.

-종자의 사전 발아

멸균 수 12 ㎖을 함유하는 9 ㎝ 옵틸룩스 페트리디쉬(Falcon)에서 24 시간까지 밤새 약한 광

-아라비도프시스 식물의 생장

종자를 ±125 ㎖의 식물 배지를 함유하는 팔콘의 인터그리드 티슈 컬쳐(Intergrid Tissue Culture) 디스크에 파종한다: 1 종자/격자.

식물을 24 ℃, 30 μ 아인슈타인 s^-1m^-2, 16 시간 명-8 시간 암으로 약 18 일간(웃자라기 전에) 생장시킨다.

단서: 상기 분석을 수행하기 위해서 1 g의 식물 물질(뿌리가 없는 새싹)/주/조건이 필요하다. 1 g 새싹은 40 내지 60 개의 식물에 상응한다.

스트레스의 도입

패러콰트

-아라비도프시스 새싹(뿌리 없음)을 수확한다

- 각각 0, 5 및 10 μM의 패러콰트를 함유하는 배양 배지(새싹은 잠겨야하지만 진공 침투되지는 않는다)에 새싹 1 g을 넣는다.

배양 배지: 팔콘의 인터그리드 티슈 컬쳐 디스크(nr. 3025) 중의 ±150 ㎖

-16 시간의 광 주기로 ±24 시간 및 30 내지 50 μ 아인슈타인 s^-1m^-2에서 암실에서 24 ℃에서 배양한다.

강한 광

-상기 플레이트의 절반을 강한 광(250 μ 아인슈타인 s^-1m^-2)으로 옮기고 4 내지 20 시간 동안 배양한다.

XTT-분석

-아가 플레이트(강한 광 스트레스) 또는 액체 배양 배지(패러콰트 스트레스)로부터 새싹(뿌리 없음)을 수확하고 상기를 반응 완충액(XTT 없음)을 함유하는 50 ㎖ 팔콘 튜브에 넣는다

-반응 완충액을 XTT(15 ㎖/gr)를 함유하는 완충액으로 대체한다

-새싹은 잠겨야하지만, 진공 침투되지는 않는다

-26 ℃에서 암실에서 배양한다

-상기 반응에 이어서 470 ㎚에서 상기 반응 배지의 흡수를 측정한다(약 1 시간).

실시예 4: 효모 니코틴아미다제 ( Pnc1 ) 유전자를 과발현하는 아라비도프시스 탈리아 나 식물의 증가된 오존 허용성

키메릭 벡터 pTVE467(실시예 1)을 에이 탈리아나 생태형 콜럼비아의 형질전환에 사용하였다. 1 차 형질전환체들을 서던-DNA- 및 노던-RNA-블럿 분석에 의해 분석하였다. 하나의 트랜스제닉 주가 Pnc1-트랜스유전자 구조물의 단일 사본을 지니고 높은 정상 상태 수준의 트랜스제닉 전장 Pnc1-mRNA(20 pg/5 ㎍ 총 RNA)를 갖는 것으로 확인되었다.

100 개의 개별적인 식물의 발아 후 6 주째에 대조군으로서 단일 사본 트랜스제닉 주 및 야생형 콜럼비아를 각각 훈증 챔버에서 오존에 노출시켰다. 2일의 연속 기간 동안 상기 식물들을 각각 250, 350 및 500 ppb의 오존 농도로 5 시간/일간 처리하였다. 처리 후에 모든 식물들을 괴사성 병변으로서 나타나는 오존 손상에 대해 가시적으로 선별하였다. 결과를 표 2에 요약한다. 500 ppb 오존 노출에서 거의 모든 식물들은 괴사성 병변을 나타낸 반면 상기 2 개의 보다 낮은 오존 농도에서는 현저하게 더 낮은 퍼센트의 트랜스제닉 식물이 손상받았다.

또한, 생명력 성능 지수(PI)의 점진적 변화를 증가하는 오존 농도 하에서 모든 트랜스제닉 주의 식물 및 야생형 식물에 대해 측정하였다. PI를 식: PI = (ABS/CS) x (TR/CS) x (ET/CS)(이때 ABS는 안테나 안료 Chl^*에 의해 흡수된 광자의 선속이고; CS는 횡단면이고; TR은 반응 중심에 의해 포착된 에너지이고 산화환원 에너지로 전환되며; ET는 더욱 하향의 전자 선속으로 CO₂ 고정을 유도한다)에 의해 계산할 수 있다. 트랜스제닉 주에서, 상기 생명력 성능 지수 PI는 증가하는 오존 농도에 따라 현저하게 증가하는 반면 상기 지수는 증가하는 오존 농도로 처리된 야생형 식물에서 일정하게 남아있는다. 이는 상기 오존 손상을 중화하는 트랜스제닉 주 내의 생리학적 보상 반응에 의해 설명될 수 있다.

더욱 또한, 대조용 식물, 상기 키메릭 Pnc1 유전자를 포함하는 식물의 동형 접합 트랜스제닉 집단뿐만 아니라 이형 접합 트랜스제닉 집단에 오존 훈증을 가하고 훈증되지 않은 식물과 비교하여 가시적인 손상 및 다양한 생리학적 반응들을 기록하였다. 상기 평가는 조정되지 않은 형광, 조정된 형광의 측정, 엽록소 측정 및 새로운 중량 측정을 포함하였다.

상기 가시적인 손상 및 생리학적 반응들을 근거로, 각 집단의 순위를 정하였으며 이는 상기 오존 영향의 정도를 가리킨다. 상기 평가에서 음수가 클수록 오존에 대한 상기 집단의 반응은 더 민감하다.

상기 대조용 비 트랜스제닉 집단 및 이형 접합 트랜스제닉 집단이 -13의 누적 점수를 갖는 반면, 상기 2 개의 동형 접합 트랜스제닉 집단은 각각 -6 및 -2의 점수를 가졌다. 따라서, 상기 동형 접합 트랜스제닉 집단이 대조용 식물보다 통계학적으로 유의적으로 더 양호하게 수행됨이 분명하다.

SEQUENCE LISTING <110> Bayer BioScience N.V. De Block, Marc Metzlaff, Michael Gossele, Veronique <120> Stress Resistant Plants <130> BCS04-2005 WO1 <150> EP 04077624.7 <151> 2004-09-24 <150> US 60/628826 <151> 2004-11-17 <160> 34 <170> PatentIn version 3.0 <210> 1 <211> 651 <212> DNA <213> Saccharomyces cerevisiae <400> 1 atgaagactt taattgttgt tgatatgcaa aatgatttta tttcaccttt aggttccttg 60 actgttccaa aaggtgagga attaatcaat cctatctcgg atttgatgca agatgctgat 120 agagactggc acaggattgt ggtcaccaga gattggcacc cttccagaca tatttcgttc 180 gcaaagaacc ataaagataa agaaccctat tcaacataca cctaccactc tccaaggcca 240 ggcgatgatt ccacgcaaga gggtattttg tggcccgtac actgtgtgaa aaacacctgg 300 ggtagtcaat tggttgacca aataatggac caagtggtca ctaagcatat taagattgtc 360 gacaagggtt tcttgactga ccgtgaatac tactccgcct tccacgacat ctggaacttc 420 cataagaccg acatgaacaa gtacttagaa aagcatcata cagacgaggt ttacattgtc 480 ggtgtagctt tggagtattg tgtcaaagcc accgccattt ccgctgcaga actaggttat 540 aagaccactg tcctgctgga ttacacaaga cccatcagcg atgatcccga agtcatcaat 600 aaggttaagg aagagttgaa ggcccacaac atcaatgtcg tggataaata a 651 <210> 2 <211> 216 <212> PRT <213> Saccharomyces cerevisiae <400> 2 Met Lys Thr Leu Ile Val Val Asp Met Gln Asn Asp Phe Ile Ser Pro 1 5 10 15 Leu Gly Ser Leu Thr Val Pro Lys Gly Glu Glu Leu Ile Asn Pro Ile 20 25 30 Ser Asp Leu Met Gln Asp Ala Asp Arg Asp Trp His Arg Ile Val Val 35 40 45 Thr Arg Asp Trp His Pro Ser Arg His Ile Ser Phe Ala Lys Asn His 50 55 60 Lys Asp Lys Glu Pro Tyr Ser Thr Tyr Thr Tyr His Ser Pro Arg Pro 65 70 75 80 Gly Asp Asp Ser Thr Gln Glu Gly Ile Leu Trp Pro Val His Cys Val 85 90 95 Lys Asn Thr Trp Gly Ser Gln Leu Val Asp Gln Ile Met Asp Gln Val 100 105 110 Val Thr Lys His Ile Lys Ile Val Asp Lys Gly Phe Leu Thr Asp Arg 115 120 125 Glu Tyr Tyr Ser Ala Phe His Asp Ile Trp Asn Phe His Lys Thr Asp 130 135 140 Met Asn Lys Tyr Leu Glu Lys His His Thr Asp Glu Val Tyr Ile Val 145 150 155 160 Gly Val Ala Leu Glu Tyr Cys Val Lys Ala Thr Ala Ile Ser Ala Ala 165 170 175 Glu Leu Gly Tyr Lys Thr Thr Val Leu Leu Asp Tyr Thr Arg Pro Ile 180 185 190 Ser Asp Asp Pro Glu Val Ile Asn Lys Val Lys Glu Glu Leu Lys Ala 195 200 205 His Asn Ile Asn Val Val Asp Lys 210 215 <210> 3 <211> 1290 <212> DNA <213> Saccharomyces cerevisiae <400> 3 ttaggtccat ctgtgcgctt cgttatcacc actccaactt cgttcagtat atcccaattc 60 ctctttcact ctcttcacag tggcaggatc tcccatattt ttacctaagt tatcagaaat 120 tttgatagcg tgattaccat ttacttctaa tagtttgata acgatgttta acggctcact 180 tttaacctgg ggttctgact tcttacgaaa atcattagta aagtttgtgc caataccgaa 240 tgtggctagc attccattct ctttagctgc atgggagtaa gttattgcct tttcgacgtt 300 caaagaatcg gaataacaga taatcttcga gaatttaggc aatttcaaca cgtcatggta 360 atggtgggaa atctttttgg tatactcaac tgggtctcca gaatcttgtc taacaccgac 420 gtaagcatca gaatatggtg gacggaatga ttttaaaaag tcatcagttc caaaagtatc 480 cgttaatgct aaaccagcat tttttgcacc aaaagtattg atccaacaat ccattgcatt 540 tttattggca tgcaaataat cttcactaat agaagcgact cccataaccc actcgtgagc 600 cacagtaccg attggcttga ctccatattt cttggcaaat aaaatatttg atgtgcctaa 660 taatagcgat ttgtttctgt ctgggttacc gttcacagct ttcatgattc cttgcataat 720 tagatcttga gccttcagag atctacgacg tcttgtacca aattcactga atctaatacc 780 attatcaaac aaagtttccg ccttcttctc agcttgttct aattggtttt cgtagtccca 840 gtcgatgtca acaaatttaa aatacgcttc tgatattagg gacagtaagg ggatctcata 900 aaggatagta tccttccaac taccactgac taaaattttc aatttgtagt gggtgggctt 960 gccctcgatt tcttctgaag tgaaggaaat ctgctcttca gggtgtagtt tgtaattaga 1020 actgctaata tacttaatat atgccgatgg caaatatggg atttcctgtt ttaagtattc 1080 aatttcctct tctgtgaacc tcaaatttcc caaatacgaa aattgctctt tcaaccaatt 1140 aatggcttcc ttattgaagg tcaattggga cgacctgttg gtatatttat aagtaactgt 1200 aacatctgga aaattagtga agacagcagc atgcatcgta atcttgtaca tgtctgtgtc 1260 caaaagagac tttatcactg gttctgacat 1290 <210> 4 <211> 429 <212> PRT <213> Saccharomyces cerevisiae <400> 4 Met Ser Glu Pro Val Ile Lys Ser Leu Leu Asp Thr Asp Met Tyr Lys 1 5 10 15 Ile Thr Met His Ala Ala Val Phe Thr Asn Phe Pro Asp Val Thr Val 20 25 30 Thr Tyr Lys Tyr Thr Asn Arg Ser Ser Gln Leu Thr Phe Asn Lys Glu 35 40 45 Ala Ile Asn Trp Leu Lys Glu Gln Phe Ser Tyr Leu Gly Asn Leu Arg 50 55 60 Phe Thr Glu Glu Glu Ile Glu Tyr Leu Lys Gln Glu Ile Pro Tyr Leu 65 70 75 80 Pro Ser Ala Tyr Ile Lys Tyr Ile Ser Ser Ser Asn Tyr Lys Leu His 85 90 95 Pro Glu Glu Gln Ile Ser Phe Thr Ser Glu Glu Ile Glu Gly Lys Pro 100 105 110 Thr His Tyr Lys Leu Lys Ile Leu Val Ser Gly Ser Trp Lys Asp Thr 115 120 125 Ile Leu Tyr Glu Ile Pro Leu Leu Ser Leu Ile Ser Glu Ala Tyr Phe 130 135 140 Lys Phe Val Asp Ile Asp Trp Asp Tyr Glu Asn Gln Leu Glu Gln Ala 145 150 155 160 Glu Lys Lys Ala Glu Thr Leu Phe Asp Asn Gly Ile Arg Phe Ser Glu 165 170 175 Phe Gly Thr Arg Arg Arg Arg Ser Leu Lys Ala Gln Asp Leu Ile Met 180 185 190 Gln Gly Ile Met Lys Ala Val Asn Gly Asn Pro Asp Arg Asn Lys Ser 195 200 205 Leu Leu Leu Gly Thr Ser Asn Ile Leu Phe Ala Lys Lys Tyr Gly Val 210 215 220 Lys Pro Ile Gly Thr Val Ala His Glu Trp Val Met Gly Val Ala Ser 225 230 235 240 Ile Ser Glu Asp Tyr Leu His Ala Asn Lys Asn Ala Met Asp Cys Trp 245 250 255 Ile Asn Thr Phe Gly Ala Lys Asn Ala Gly Leu Ala Leu Thr Asp Thr 260 265 270 Phe Gly Thr Asp Asp Phe Leu Lys Ser Phe Arg Pro Pro Tyr Ser Asp 275 280 285 Ala Tyr Val Gly Val Arg Gln Asp Ser Gly Asp Pro Val Glu Tyr Thr 290 295 300 Lys Lys Ile Ser His His Tyr His Asp Val Leu Lys Leu Pro Lys Phe 305 310 315 320 Ser Lys Ile Ile Cys Tyr Ser Asp Ser Leu Asn Val Glu Lys Ala Ile 325 330 335 Thr Tyr Ser His Ala Ala Lys Glu Asn Gly Met Leu Ala Thr Phe Gly 340 345 350 Ile Gly Thr Asn Phe Thr Asn Asp Phe Arg Lys Lys Ser Glu Pro Gln 355 360 365 Val Lys Ser Glu Pro Leu Asn Ile Val Ile Lys Leu Leu Glu Val Asn 370 375 380 Gly Asn His Ala Ile Lys Ile Ser Asp Asn Leu Gly Lys Asn Met Gly 385 390 395 400 Asp Pro Ala Thr Val Lys Arg Val Lys Glu Glu Leu Gly Tyr Thr Glu 405 410 415 Arg Ser Trp Ser Gly Asp Asn Glu Ala His Arg Trp Thr 420 425 <210> 5 <211> 1206 <212> DNA <213> Saccharomyces cerevisiae <400> 5 atggatccca caagagctcc ggatttcaaa ccgccatctg cagacgagga attgattcct 60 ccacccgacc cggaatctaa aattcccaaa tctattccaa ttattccata cgtcttagcc 120 gatgcgaatt cctctataga tgcacctttt aatattaaga ggaagaaaaa gcatcctaag 180 catcatcatc accatcatca cagtcgtaaa gaaggcaatg ataaaaaaca tcagcatatt 240 ccattgaacc aagacgactt tcaaccactt tccgcagaag tgtcttccga agatgatgac 300 gcggatttta gatccaagga gagatacggt tcagattcaa ccacagaatc agaaactaga 360 ggtgttcaga aatatcagat tgctgattta gaagaagttc cacatggaat cgttcgtcaa 420 gcaagaacct tggaagacta cgaattcccc tcacacagat tatcgaaaaa attactggat 480 ccaaataaac tgccgttagt aatagtagca tgtgggtctt tttcaccaat cacctacttg 540 catctaagaa tgtttgaaat ggctttagat gcaatctctg aacaaacaag gtttgaagtc 600 ataggtggat attactcccc tgttagtgat aactatcaaa agcaaggctt ggccccatcc 660 taccatagag tacgtatgtg tgaattggcc tgcgaaagaa cctcatcttg gttgatggtg 720 gatgcatggg agtcattgca accttcatac acaagaactg ccaaggtctt ggatcatttc 780 aatcacgaaa tcaatattaa gagaggtggt gtagctactg ttactggaga aaaaattggt 840 gtgaaaataa tgttgctggc tggtggtgac ctaatagagt caatgggtga accaaacgtt 900 tgggcggacg ccgatttaca tcacattctc ggtaattacg gttgtttgat tgtcgaacgt 960 actggttctg atgtaaggtc ttttttgtta tcccatgata ttatgtatga acatagaagg 1020 aatattctta tcatcaagca actcatctat aatgatattt cttccacgaa agttcgtcta 1080 tttatcagac gcgccatgtc tgtacaatat ttgttaccta attcggtcat caggtatatc 1140 caagaacata gactatatgt ggaccaaacc gaacctgtta agcaagttct tggaaacaaa 1200 gaatga 1206 <210> 6 <211> 401 <212> PRT <213> Saccharomyces cerevisiae <400> 6 Met Asp Pro Thr Arg Ala Pro Asp Phe Lys Pro Pro Ser Ala Asp Glu 1 5 10 15 Glu Leu Ile Pro Pro Pro Asp Pro Glu Ser Lys Ile Pro Lys Ser Ile 20 25 30 Pro Ile Ile Pro Tyr Val Leu Ala Asp Ala Asn Ser Ser Ile Asp Ala 35 40 45 Pro Phe Asn Ile Lys Arg Lys Lys Lys His Pro Lys His His His His 50 55 60 His His His Ser Arg Lys Glu Gly Asn Asp Lys Lys His Gln His Ile 65 70 75 80 Pro Leu Asn Gln Asp Asp Phe Gln Pro Leu Ser Ala Glu Val Ser Ser 85 90 95 Glu Asp Asp Asp Ala Asp Phe Arg Ser Lys Glu Arg Tyr Gly Ser Asp 100 105 110 Ser Thr Thr Glu Ser Glu Thr Arg Gly Val Gln Lys Tyr Gln Ile Ala 115 120 125 Asp Leu Glu Glu Val Pro His Gly Ile Val Arg Gln Ala Arg Thr Leu 130 135 140 Glu Asp Tyr Glu Phe Pro Ser His Arg Leu Ser Lys Lys Leu Leu Asp 145 150 155 160 Pro Asn Lys Leu Pro Leu Val Ile Val Ala Cys Gly Ser Phe Ser Pro 165 170 175 Ile Thr Tyr Leu His Leu Arg Met Phe Glu Met Ala Leu Asp Ala Ile 180 185 190 Ser Glu Gln Thr Arg Phe Glu Val Ile Gly Gly Tyr Tyr Ser Pro Val 195 200 205 Ser Asp Asn Tyr Gln Lys Gln Gly Leu Ala Pro Ser Tyr His Arg Val 210 215 220 Arg Met Cys Glu Leu Ala Cys Glu Arg Thr Ser Ser Trp Leu Met Val 225 230 235 240 Asp Ala Trp Glu Ser Leu Gln Pro Ser Tyr Thr Arg Thr Ala Lys Val 245 250 255 Leu Asp His Phe Asn His Glu Ile Asn Ile Lys Arg Gly Gly Val Ala 260 265 270 Thr Val Thr Gly Glu Lys Ile Gly Val Lys Ile Met Leu Leu Ala Gly 275 280 285 Gly Asp Leu Ile Glu Ser Met Gly Glu Pro Asn Val Trp Ala Asp Ala 290 295 300 Asp Leu His His Ile Leu Gly Asn Tyr Gly Cys Leu Ile Val Glu Arg 305 310 315 320 Thr Gly Ser Asp Val Arg Ser Phe Leu Leu Ser His Asp Ile Met Tyr 325 330 335 Glu His Arg Arg Asn Ile Leu Ile Ile Lys Gln Leu Ile Tyr Asn Asp 340 345 350 Ile Ser Ser Thr Lys Val Arg Leu Phe Ile Arg Arg Ala Met Ser Val 355 360 365 Gln Tyr Leu Leu Pro Asn Ser Val Ile Arg Tyr Ile Gln Glu His Arg 370 375 380 Leu Tyr Val Asp Gln Thr Glu Pro Val Lys Gln Val Leu Gly Asn Lys 385 390 395 400 Glu <210> 7 <211> 1188 <212> DNA <213> Saccharomyces cerevisiae <400> 7 atggatccca ccaaagcacc cgattttaaa ccgccacagc caaatgaaga actacaacca 60 ccgccagatc caacacatac gataccaaaa tctggaccca tagttccata tgttttagct 120 gattataatt cttcgatcga tgctcctttc aatctcgaca tttacaaaac cctgtcgtca 180 aggaaaaaaa acgccaactc aagcaaccga atggaccata ttccattaaa tactagtgac 240 ttccagccac tatctcggga tgtatcatcg gaggaggaaa gtgaagggca atcgaatgga 300 attgacgcta ctctacagga tgttacgatg actgggaatt tgggggtact gaagagccaa 360 attgctgatt tggaagaagt tcctcacaca attgtaagac aagccagaac tattgaagat 420 tacgaatttc ctgtacacag attgacgaaa aagttacaag atcctgaaaa actgcctctg 480 atcatcgttg cttgtggatc attttctccc ataacatacc tacatttgag aatgtttgaa 540 atggctttag atgatatcaa tgagcaaacg cgttttgaag tggttggtgg ttatttttct 600 ccagtaagtg ataactatca aaagcgaggg ttagccccag cttatcatcg tgtccgcatg 660 tgcgaattag catgcgagcg gacatcatct tggttaatgg ttgatgcctg ggaatcttta 720 caatcaagtt atacaaggac agcaaaagtc ttggaccatt tcaatcatga aataaatatc 780 aagagaggtg gaatcatgac tgtagatggt gaaaaaatgg gcgtaaaaat catgttattg 840 gcaggcggtg atcttatcga atccatgggc gagcctcatg tgtgggctga ttcagacctg 900 caccatattt tgggtaatta tggatgtttg atcgtggaaa ggactggttc tgatgttagg 960 tccttcttgc tttcccatga tatcatgtat gaacacagaa gaaatatcct tattatcaaa 1020 caacttattt acaatgatat ttcctctacg aaagtgcggc ttttcatcag acgtggaatg 1080 tcagttcaat atcttcttcc aaactctgtc atccgttaca tccaagagta taatctatac 1140 attaatcaaa gtgaaccggt caagcaggtc ttggatagca aagagtga 1188 <210> 8 <211> 395 <212> PRT <213> Saccharomyces cerevisiae <400> 8 Met Asp Pro Thr Lys Ala Pro Asp Phe Lys Pro Pro Gln Pro Asn Glu 1 5 10 15 Glu Leu Gln Pro Pro Pro Asp Pro Thr His Thr Ile Pro Lys Ser Gly 20 25 30 Pro Ile Val Pro Tyr Val Leu Ala Asp Tyr Asn Ser Ser Ile Asp Ala 35 40 45 Pro Phe Asn Leu Asp Ile Tyr Lys Thr Leu Ser Ser Arg Lys Lys Asn 50 55 60 Ala Asn Ser Ser Asn Arg Met Asp His Ile Pro Leu Asn Thr Ser Asp 65 70 75 80 Phe Gln Pro Leu Ser Arg Asp Val Ser Ser Glu Glu Glu Ser Glu Gly 85 90 95 Gln Ser Asn Gly Ile Asp Ala Thr Leu Gln Asp Val Thr Met Thr Gly 100 105 110 Asn Leu Gly Val Leu Lys Ser Gln Ile Ala Asp Leu Glu Glu Val Pro 115 120 125 His Thr Ile Val Arg Gln Ala Arg Thr Ile Glu Asp Tyr Glu Phe Pro 130 135 140 Val His Arg Leu Thr Lys Lys Leu Gln Asp Pro Glu Lys Leu Pro Leu 145 150 155 160 Ile Ile Val Ala Cys Gly Ser Phe Ser Pro Ile Thr Tyr Leu His Leu 165 170 175 Arg Met Phe Glu Met Ala Leu Asp Asp Ile Asn Glu Gln Thr Arg Phe 180 185 190 Glu Val Val Gly Gly Tyr Phe Ser Pro Val Ser Asp Asn Tyr Gln Lys 195 200 205 Arg Gly Leu Ala Pro Ala Tyr His Arg Val Arg Met Cys Glu Leu Ala 210 215 220 Cys Glu Arg Thr Ser Ser Trp Leu Met Val Asp Ala Trp Glu Ser Leu 225 230 235 240 Gln Ser Ser Tyr Thr Arg Thr Ala Lys Val Leu Asp His Phe Asn His 245 250 255 Glu Ile Asn Ile Lys Arg Gly Gly Ile Met Thr Val Asp Gly Glu Lys 260 265 270 Met Gly Val Lys Ile Met Leu Leu Ala Gly Gly Asp Leu Ile Glu Ser 275 280 285 Met Gly Glu Pro His Val Trp Ala Asp Ser Asp Leu His His Ile Leu 290 295 300 Gly Asn Tyr Gly Cys Leu Ile Val Glu Arg Thr Gly Ser Asp Val Arg 305 310 315 320 Ser Phe Leu Leu Ser His Asp Ile Met Tyr Glu His Arg Arg Asn Ile 325 330 335 Leu Ile Ile Lys Gln Leu Ile Tyr Asn Asp Ile Ser Ser Thr Lys Val 340 345 350 Arg Leu Phe Ile Arg Arg Gly Met Ser Val Gln Tyr Leu Leu Pro Asn 355 360 365 Ser Val Ile Arg Tyr Ile Gln Glu Tyr Asn Leu Tyr Ile Asn Gln Ser 370 375 380 Glu Pro Val Lys Gln Val Leu Asp Ser Lys Glu 385 390 395 <210> 9 <211> 2145 <212> DNA <213> Saccharomyces cerevisiae <400> 9 atgtcacatc ttatcacttt agctacatgc aacttgaatc aatgggccct agattttgaa 60 ggtaatagag accgtatcct acagtccatt aagattgcca aagagagggg tgccaggtta 120 cgtgtcggcc cagaactgga aataactggc tacggatgtt tagatcattt tttagaaaat 180 gacgtttgcc ttcattcatg ggaaatgtat gctcaaatca ttaagaataa agaaacccat 240 ggattaatac ttgacattgg tatgcccgtt ctacacaaga atgttcgtta taattgtcgt 300 ttgttatcct tggatggtga gatattgttc ataagaccta agatttggtt agctaatgat 360 ggtaactata gggaaatgag atttttcaca ccttggatga aacctggcgt ggtggaggac 420 tttatccttc cacctgagat tcagaaagtt accggccaga gacttgtgcc atttggggac 480 gctgtgataa attcattgga tacatgcatt ggtacagaaa cttgtgaaga attgtttaca 540 cctcaatccc cccacatcgc catgtcttta gatggtgtgg aaatcatgac aaactcatct 600 ggttctcatc atgaactgcg taagttaaat aaaaggttag acctaatttt aaatgccact 660 aaacgttgtg gtggtgttta cttgtatgca aatcaaagag gttgtgatgg tgacagatta 720 tattatgatg gctgtgcact aattgccatc aatggtacaa ttgtagccca aggttcacaa 780 ttttcgctag atgatgtgga agtagttact gctactgtgg acctagaaga ggtgaggagt 840 tatcgtgcag ctgtcatgtc tcgtggccta caagcctcct tggcagaaat aaagttcaag 900 cgtattgata ttcctgtaga attggcttta atgacctcca gatttgatcc tacagtgtgt 960 ccaacaaaag tccgcgagcc tttctatcac tctcctgagg aagaaattgc actgggacct 1020 gcttgctgga tgtgggatta tttaagacgt tgtaacggaa cagggttttt ccttccctta 1080 tctgggggca ttgactcttg tgcaactgca atgattgtcc actctatgtg ccgtttagtg 1140 accgacgctg ctcaaaatgg aaatgagcaa gttatcaaag acgttcgtaa gataacacgt 1200 agcggcgatg attggattcc agacagtcca caggatctag cctcaaaaat atttcactcc 1260 tgtttcatgg gtacggaaaa ttcatccaag gagacaagaa acagagcaaa ggacctttcc 1320 aatgcaattg gatcttacca cgtggattta aagatggact cattggtatc cagtgtggtg 1380 tccttattcg aagtagccac tggcaaaaaa ccaatataca aaatatttgg gggatctcaa 1440 atcgagaact tggctttaca aaacatccag gcgcgtctaa gaatggttct ttcttatctt 1500 tttgcgcaac tgttgccgtg ggttcgtggt atcccaaact cgggtggatt gttagtactt 1560 ggtagcgcaa atgttgatga gtgcttacgt gggtatctaa caaaatatga ctgctcctcc 1620 gcagatatca accctattgg gggtatttca aaaactgact tgaaaagatt cattgcctac 1680 gcatcaaaac aatataacat gccaatcttg aatgactttt taaacgctac accaactgca 1740 gaattagaac ctatgactaa agattacgtt caatcggatg agatagatat ggggatgacg 1800 tatgaagaat tgggcgtgtt tggttaccta agaaaggttg aaaaatgtgg tccttattct 1860 atgttcttaa aacttcttca tcaatggtcc ccaaagttaa cacctcgtca aatatctgaa 1920 aaggtgaaaa gatttttctt cttctatgcc atcaacagac acaagcaaac tgttttaact 1980 cctagttatc atgctgaaca gtattcacca gaagacaaca gatttgactt acgtcctttc 2040 ttaatcaacc caagatttcc atgggcttca agaaaaattg atgaagttgt cgagcagtgt 2100 gaagcacata aaggctcaac gcttgacatt atgtctattg attag 2145 <210> 10 <211> 714 <212> PRT <213> Saccharomyces cerevisiae <400> 10 Met Ser His Leu Ile Thr Leu Ala Thr Cys Asn Leu Asn Gln Trp Ala 1 5 10 15 Leu Asp Phe Glu Gly Asn Arg Asp Arg Ile Leu Gln Ser Ile Lys Ile 20 25 30 Ala Lys Glu Arg Gly Ala Arg Leu Arg Val Gly Pro Glu Leu Glu Ile 35 40 45 Thr Gly Tyr Gly Cys Leu Asp His Phe Leu Glu Asn Asp Val Cys Leu 50 55 60 His Ser Trp Glu Met Tyr Ala Gln Ile Ile Lys Asn Lys Glu Thr His 65 70 75 80 Gly Leu Ile Leu Asp Ile Gly Met Pro Val Leu His Lys Asn Val Arg 85 90 95 Tyr Asn Cys Arg Leu Leu Ser Leu Asp Gly Glu Ile Leu Phe Ile Arg 100 105 110 Pro Lys Ile Trp Leu Ala Asn Asp Gly Asn Tyr Arg Glu Met Arg Phe 115 120 125 Phe Thr Pro Trp Met Lys Pro Gly Val Val Glu Asp Phe Ile Leu Pro 130 135 140 Pro Glu Ile Gln Lys Val Thr Gly Gln Arg Leu Val Pro Phe Gly Asp 145 150 155 160 Ala Val Ile Asn Ser Leu Asp Thr Cys Ile Gly Thr Glu Thr Cys Glu 165 170 175 Glu Leu Phe Thr Pro Gln Ser Pro His Ile Ala Met Ser Leu Asp Gly 180 185 190 Val Glu Ile Met Thr Asn Ser Ser Gly Ser His His Glu Leu Arg Lys 195 200 205 Leu Asn Lys Arg Leu Asp Leu Ile Leu Asn Ala Thr Lys Arg Cys Gly 210 215 220 Gly Val Tyr Leu Tyr Ala Asn Gln Arg Gly Cys Asp Gly Asp Arg Leu 225 230 235 240 Tyr Tyr Asp Gly Cys Ala Leu Ile Ala Ile Asn Gly Thr Ile Val Ala 245 250 255 Gln Gly Ser Gln Phe Ser Leu Asp Asp Val Glu Val Val Thr Ala Thr 260 265 270 Val Asp Leu Glu Glu Val Arg Ser Tyr Arg Ala Ala Val Met Ser Arg 275 280 285 Gly Leu Gln Ala Ser Leu Ala Glu Ile Lys Phe Lys Arg Ile Asp Ile 290 295 300 Pro Val Glu Leu Ala Leu Met Thr Ser Arg Phe Asp Pro Thr Val Cys 305 310 315 320 Pro Thr Lys Val Arg Glu Pro Phe Tyr His Ser Pro Glu Glu Glu Ile 325 330 335 Ala Leu Gly Pro Ala Cys Trp Met Trp Asp Tyr Leu Arg Arg Cys Asn 340 345 350 Gly Thr Gly Phe Phe Leu Pro Leu Ser Gly Gly Ile Asp Ser Cys Ala 355 360 365 Thr Ala Met Ile Val His Ser Met Cys Arg Leu Val Thr Asp Ala Ala 370 375 380 Gln Asn Gly Asn Glu Gln Val Ile Lys Asp Val Arg Lys Ile Thr Arg 385 390 395 400 Ser Gly Asp Asp Trp Ile Pro Asp Ser Pro Gln Asp Leu Ala Ser Lys 405 410 415 Ile Phe His Ser Cys Phe Met Gly Thr Glu Asn Ser Ser Lys Glu Thr 420 425 430 Arg Asn Arg Ala Lys Asp Leu Ser Asn Ala Ile Gly Ser Tyr His Val 435 440 445 Asp Leu Lys Met Asp Ser Leu Val Ser Ser Val Val Ser Leu Phe Glu 450 455 460 Val Ala Thr Gly Lys Lys Pro Ile Tyr Lys Ile Phe Gly Gly Ser Gln 465 470 475 480 Ile Glu Asn Leu Ala Leu Gln Asn Ile Gln Ala Arg Leu Arg Met Val 485 490 495 Leu Ser Tyr Leu Phe Ala Gln Leu Leu Pro Trp Val Arg Gly Ile Pro 500 505 510 Asn Ser Gly Gly Leu Leu Val Leu Gly Ser Ala Asn Val Asp Glu Cys 515 520 525 Leu Arg Gly Tyr Leu Thr Lys Tyr Asp Cys Ser Ser Ala Asp Ile Asn 530 535 540 Pro Ile Gly Gly Ile Ser Lys Thr Asp Leu Lys Arg Phe Ile Ala Tyr 545 550 555 560 Ala Ser Lys Gln Tyr Asn Met Pro Ile Leu Asn Asp Phe Leu Asn Ala 565 570 575 Thr Pro Thr Ala Glu Leu Glu Pro Met Thr Lys Asp Tyr Val Gln Ser 580 585 590 Asp Glu Ile Asp Met Gly Met Thr Tyr Glu Glu Leu Gly Val Phe Gly 595 600 605 Tyr Leu Arg Lys Val Glu Lys Cys Gly Pro Tyr Ser Met Phe Leu Lys 610 615 620 Leu Leu His Gln Trp Ser Pro Lys Leu Thr Pro Arg Gln Ile Ser Glu 625 630 635 640 Lys Val Lys Arg Phe Phe Phe Phe Tyr Ala Ile Asn Arg His Lys Gln 645 650 655 Thr Val Leu Thr Pro Ser Tyr His Ala Glu Gln Tyr Ser Pro Glu Asp 660 665 670 Asn Arg Phe Asp Leu Arg Pro Phe Leu Ile Asn Pro Arg Phe Pro Trp 675 680 685 Ala Ser Arg Lys Ile Asp Glu Val Val Glu Gln Cys Glu Ala His Lys 690 695 700 Gly Ser Thr Leu Asp Ile Met Ser Ile Asp 705 710 <210> 11 <211> 597 <212> DNA <213> Arabidopsis thaliana <400> 11 atggcttcct catcaacgag aaagtacgag acacgaaagc gagatccaaa ctctaaaatc 60 gcagctcttc tcgttatcga catgcagaat cacttctcct ccatggccaa acccatcctc 120 aacaacgttc tcaccaccat cgacatctgc cgacgcgcct cagtccccgt attctttacg 180 cgtcacaacc acaaatcccc gaccgaccac ggcatgctcg gcgagtggtg taacggcgat 240 gtaatccttg acggaaccac cgattctgaa atcatccagg agatacaagg ccaagtaacc 300 ggaccagacg agatggtgga gaagaacacg tacagtgcgt ttaacaaaac ccgcctccag 360 gaaaacctgg aaaagatcgg agtaaaggag gtgatcgtga tcggagtgat gacgaacttg 420 tgctgtgaga caacggcgcg tgaagcgttt attaagggtt ttagggtttt tttctcgacg 480 gacgcgactg cgacgtttaa tgaggagctt cacgaggcta cgctaatgaa tctcgctttt 540 ggcttcgctt atctcgtcga ttgcgataaa ctccggcgaa gtctactcgg taactaa 597 <210> 12 <211> 198 <212> PRT <213> Arabidopsis thaliana <400> 12 Met Ala Ser Ser Ser Thr Arg Lys Tyr Glu Thr Arg Lys Arg Asp Pro 1 5 10 15 Asn Ser Lys Ile Ala Ala Leu Leu Val Ile Asp Met Gln Asn His Phe 20 25 30 Ser Ser Met Ala Lys Pro Ile Leu Asn Asn Val Leu Thr Thr Ile Asp 35 40 45 Ile Cys Arg Arg Ala Ser Val Pro Val Phe Phe Thr Arg His Asn His 50 55 60 Lys Ser Pro Thr Asp His Gly Met Leu Gly Glu Trp Cys Asn Gly Asp 65 70 75 80 Val Ile Leu Asp Gly Thr Thr Asp Ser Glu Ile Ile Gln Glu Ile Gln 85 90 95 Gly Gln Val Thr Gly Pro Asp Glu Met Val Glu Lys Asn Thr Tyr Ser 100 105 110 Ala Phe Asn Lys Thr Arg Leu Gln Glu Asn Leu Glu Lys Ile Gly Val 115 120 125 Lys Glu Val Ile Val Ile Gly Val Met Thr Asn Leu Cys Cys Glu Thr 130 135 140 Thr Ala Arg Glu Ala Phe Ile Lys Gly Phe Arg Val Phe Phe Ser Thr 145 150 155 160 Asp Ala Thr Ala Thr Phe Asn Glu Glu Leu His Glu Ala Thr Leu Met 165 170 175 Asn Leu Ala Phe Gly Phe Ala Tyr Leu Val Asp Cys Asp Lys Leu Arg 180 185 190 Arg Ser Leu Leu Gly Asn 195 <210> 13 <211> 597 <212> DNA <213> Arabidopsis thaliana <400> 13 atggcttctt catcatcgag aacgtacgag acacgaaagc gagagccaaa tcctaaaatc 60 gcagctcttc tcgtcatcga tatgcagaat cacttctact ctatggctga accaatcctc 120 caaaacgctc tcaccaccat cgacatctgc cgacgcgctt caatccccgt attcttcacg 180 cgccacaacc acaaatcccc aaccgaccac ggcatgctcg gagagtggtg gaacggcgat 240 ctaatcctcg acggaaccac tgattccgaa atcatcccgg aaatcaatcg ccaggtcacc 300 ggaccagacg aaatcgtgga gaagagcacg tacagtgcgt ttaacaacac gcaccttcag 360 gagaagctgg acaagatcgg agtgaaggag gtgatcgtta tcggagtgat gacgaaccta 420 tgctgtgaga cgacggcgcg tgaagcgttt gtaaaggggt ttagggtttt tttctcgacg 480 gacgcgactg cgacggttaa tgaagagctt cacgaggcta ctctaatgaa tctcgcgtat 540 ggctttgctt atctcgtcga ttgcgataga ctccggcgag gtctactcag tagttaa 597 <210> 14 <211> 198 <212> PRT <213> Arabidopsis thaliana <400> 14 Met Ala Ser Ser Ser Ser Arg Thr Tyr Glu Thr Arg Lys Arg Glu Pro 1 5 10 15 Asn Pro Lys Ile Ala Ala Leu Leu Val Ile Asp Met Gln Asn His Phe 20 25 30 Tyr Ser Met Ala Glu Pro Ile Leu Gln Asn Ala Leu Thr Thr Ile Asp 35 40 45 Ile Cys Arg Arg Ala Ser Ile Pro Val Phe Phe Thr Arg His Asn His 50 55 60 Lys Ser Pro Thr Asp His Gly Met Leu Gly Glu Trp Trp Asn Gly Asp 65 70 75 80 Leu Ile Leu Asp Gly Thr Thr Asp Ser Glu Ile Ile Pro Glu Ile Asn 85 90 95 Arg Gln Val Thr Gly Pro Asp Glu Ile Val Glu Lys Ser Thr Tyr Ser 100 105 110 Ala Phe Asn Asn Thr His Leu Gln Glu Lys Leu Asp Lys Ile Gly Val 115 120 125 Lys Glu Val Ile Val Ile Gly Val Met Thr Asn Leu Cys Cys Glu Thr 130 135 140 Thr Ala Arg Glu Ala Phe Val Lys Gly Phe Arg Val Phe Phe Ser Thr 145 150 155 160 Asp Ala Thr Ala Thr Val Asn Glu Glu Leu His Glu Ala Thr Leu Met 165 170 175 Asn Leu Ala Tyr Gly Phe Ala Tyr Leu Val Asp Cys Asp Arg Leu Arg 180 185 190 Arg Gly Leu Leu Ser Ser 195 <210> 15 <211> 591 <212> DNA <213> Arabidopsis thaliana <400> 15 atggccgaga gatggaggaa cacggctcta ctcgtcatcg acatgcagaa cgatttcata 60 gaggaaggtg ctgtgacgca agtgaaagga ggaaaatcta tagttcctaa tgttatcaga 120 gtcgtcgaac tcgcgaggca gcgtggtatt ctcgtaattt gggttgttcg agaacatgat 180 cgtcaaggaa gagatgttga attattcagg cgccataact acagttctga gaaagtcggg 240 ccagttatta aaggcaccgt aggagcagaa ttggttgatg gattgatgat caacgaagaa 300 gatgactata agattgtgaa aactcgtttc agtgctttct ttagtaccaa tcttcattcc 360 ttcttgcaaa cttcaggggt taccaagtta gtgattgctg gtgtgcaaac gccgaactgt 420 atccggcaaa cggtgtttga tgcagtggcg ctggattatc ccaatgtgac tgttattaca 480 gatgccacag ctgctgcaac accagagatc catactgcga atattcttga catgaagaat 540 attggagtca agactcctac attacacgag tggtccgaag aacttgcttg a 591 <210> 16 <211> 196 <212> PRT <213> Arabidopsis thaliana <400> 16 Met Ala Glu Arg Trp Arg Asn Thr Ala Leu Leu Val Ile Asp Met Gln 1 5 10 15 Asn Asp Phe Ile Glu Glu Gly Ala Val Thr Gln Val Lys Gly Gly Lys 20 25 30 Ser Ile Val Pro Asn Val Ile Arg Val Val Glu Leu Ala Arg Gln Arg 35 40 45 Gly Ile Leu Val Ile Trp Val Val Arg Glu His Asp Arg Gln Gly Arg 50 55 60 Asp Val Glu Leu Phe Arg Arg His Asn Tyr Ser Ser Glu Lys Val Gly 65 70 75 80 Pro Val Ile Lys Gly Thr Val Gly Ala Glu Leu Val Asp Gly Leu Met 85 90 95 Ile Asn Glu Glu Asp Asp Tyr Lys Ile Val Lys Thr Arg Phe Ser Ala 100 105 110 Phe Phe Ser Thr Asn Leu His Ser Phe Leu Gln Thr Ser Gly Val Thr 115 120 125 Lys Leu Val Ile Ala Gly Val Gln Thr Pro Asn Cys Ile Arg Gln Thr 130 135 140 Val Phe Asp Ala Val Ala Leu Asp Tyr Pro Asn Val Thr Val Ile Thr 145 150 155 160 Asp Ala Thr Ala Ala Ala Thr Pro Glu Ile His Thr Ala Asn Ile Leu 165 170 175 Asp Met Lys Asn Ile Gly Val Lys Thr Pro Thr Leu His Glu Trp Ser 180 185 190 Glu Glu Leu Ala 195 <210> 17 <211> 1680 <212> DNA <213> Arabidopsis thaliana <400> 17 atggagaaga aagaaaatgg tctcgatgga aagcaatcgg gtcgggtcat taacggaccc 60 actaacccga tggtcacacc tctgctcaac gatctttacc aattcaccat ggcttatgct 120 tattggaaag ctggcaaaca atctgagcga tctgtgtttg atctgtattt tcgtaagaat 180 ccttttggtg gagaatacac tatctttgct ggtttagaag aatgcatcaa atttctcgct 240 aatttcaatt tgactgatga agagatcgat ttcgttcgtg attcgttacc tggatgtgag 300 gaagctttct gtgattatct tcgagggctt gattgttctg acattgaagt gtatgccatt 360 tcggaaggat cagttgtttt tcctaaagtt cctttactca gaatcgaagg tcctgttgct 420 gtggtgcaat tgttggaaac tccattcctc aatctcatca attacgcatc tttggttgct 480 acaaatgcag caagacatcg gtttgttgca ggaaaatcta agcttctgct tgagtttggt 540 gctagaagag ctcagggacc cgatggtgca ataagcgcat caaagtattg ctaccttgga 600 ggttttgatg caacaagtaa tgttgcagcg ggaaaactgt ttgggatacc cctccgtggt 660 actcattccc atgcttttgt tagctcattc atgagccttg atgaaattgt tgacaaagtg 720 cttcgaagtt ctgatgggaa aagcacttgt aaggatttta tatgtttggt ccaaacttgc 780 ctaacaaaga ttcagaattc atcttcatta caaggaattt tttccgagac aaatcaaagc 840 gagcttgcag cgttcatttc atatgcactg gcattcccaa actcctttct cgctcttgta 900 gacacttatg atgtgatgaa gagtggtatt ccaaacttct gtgctgttgc tctagcactt 960 aatgaattgg gatacaaagc agtaggcatt agactggatt caggtgactt agcctatctt 1020 tctactgagg tcaggaaatt cttttgtgcc atagagagag acctcaaagt tcctgatttc 1080 gggaagatga tcgtcactgc tagtaacgat ctaaacgaag agacagtcga tgctctaaat 1140 aaacagggtc atgaagtaga tgcatttgga attggaacca acttagtgac ttgctatgcg 1200 caagctgcgt taggttgtgt tttcaaactt gtggaaataa acaatcagcc tcggatcaaa 1260 ctttctgaag atgttactaa ggtatcgatt ccatgtaaaa agcgtactta cagattgttc 1320 ggaaaagagg gttaccctct tgttgatata atgactggag agaacgaacc acctccaaag 1380 gtcggtgaaa ggttactttg ccgtcatcca ttcaatgaat caaaaagggc ttatgtggtt 1440 ccacaacgcg ttgaagagct tctgaaatgt tattggcgtg gcaatgcaga tgaagctagg 1500 gaagagctag agccattgaa agagctaaga aatcgttgca tcaaacagct cgaaaatatg 1560 cgacccgatc atatgagaag attaaaccct actccttata aggttagtgt cagcgccaag 1620 ttgtatgact tcatccactt cctctggctc aacgaagctc ctgtcggtga actgcattga 1680 <210> 18 <211> 559 <212> PRT <213> Arabidopsis thaliana <400> 18 Met Glu Lys Lys Glu Asn Gly Leu Asp Gly Lys Gln Ser Gly Arg Val 1 5 10 15 Ile Asn Gly Pro Thr Asn Pro Met Val Thr Pro Leu Leu Asn Asp Leu 20 25 30 Tyr Gln Phe Thr Met Ala Tyr Ala Tyr Trp Lys Ala Gly Lys Gln Ser 35 40 45 Glu Arg Ser Val Phe Asp Leu Tyr Phe Arg Lys Asn Pro Phe Gly Gly 50 55 60 Glu Tyr Thr Ile Phe Ala Gly Leu Glu Glu Cys Ile Lys Phe Leu Ala 65 70 75 80 Asn Phe Asn Leu Thr Asp Glu Glu Ile Asp Phe Val Arg Asp Ser Leu 85 90 95 Pro Gly Cys Glu Glu Ala Phe Cys Asp Tyr Leu Arg Gly Leu Asp Cys 100 105 110 Ser Asp Ile Glu Val Tyr Ala Ile Ser Glu Gly Ser Val Val Phe Pro 115 120 125 Lys Val Pro Leu Leu Arg Ile Glu Gly Pro Val Ala Val Val Gln Leu 130 135 140 Leu Glu Thr Pro Phe Leu Asn Leu Ile Asn Tyr Ala Ser Leu Val Ala 145 150 155 160 Thr Asn Ala Ala Arg His Arg Phe Val Ala Gly Lys Ser Lys Leu Leu 165 170 175 Leu Glu Phe Gly Ala Arg Arg Ala Gln Gly Pro Asp Gly Ala Ile Ser 180 185 190 Ala Ser Lys Tyr Cys Tyr Leu Gly Gly Phe Asp Ala Thr Ser Asn Val 195 200 205 Ala Ala Gly Lys Leu Phe Gly Ile Pro Leu Arg Gly Thr His Ser His 210 215 220 Ala Phe Val Ser Ser Phe Met Ser Leu Asp Glu Ile Val Asp Lys Val 225 230 235 240 Leu Arg Ser Ser Asp Gly Lys Ser Thr Cys Lys Asp Phe Ile Cys Leu 245 250 255 Val Gln Thr Cys Leu Thr Lys Ile Gln Asn Ser Ser Ser Leu Gln Gly 260 265 270 Ile Phe Ser Glu Thr Asn Gln Ser Glu Leu Ala Ala Phe Ile Ser Tyr 275 280 285 Ala Leu Ala Phe Pro Asn Ser Phe Leu Ala Leu Val Asp Thr Tyr Asp 290 295 300 Val Met Lys Ser Gly Ile Pro Asn Phe Cys Ala Val Ala Leu Ala Leu 305 310 315 320 Asn Glu Leu Gly Tyr Lys Ala Val Gly Ile Arg Leu Asp Ser Gly Asp 325 330 335 Leu Ala Tyr Leu Ser Thr Glu Val Arg Lys Phe Phe Cys Ala Ile Glu 340 345 350 Arg Asp Leu Lys Val Pro Asp Phe Gly Lys Met Ile Val Thr Ala Ser 355 360 365 Asn Asp Leu Asn Glu Glu Thr Val Asp Ala Leu Asn Lys Gln Gly His 370 375 380 Glu Val Asp Ala Phe Gly Ile Gly Thr Asn Leu Val Thr Cys Tyr Ala 385 390 395 400 Gln Ala Ala Leu Gly Cys Val Phe Lys Leu Val Glu Ile Asn Asn Gln 405 410 415 Pro Arg Ile Lys Leu Ser Glu Asp Val Thr Lys Val Ser Ile Pro Cys 420 425 430 Lys Lys Arg Thr Tyr Arg Leu Phe Gly Lys Glu Gly Tyr Pro Leu Val 435 440 445 Asp Ile Met Thr Gly Glu Asn Glu Pro Pro Pro Lys Val Gly Glu Arg 450 455 460 Leu Leu Cys Arg His Pro Phe Asn Glu Ser Lys Arg Ala Tyr Val Val 465 470 475 480 Pro Gln Arg Val Glu Glu Leu Leu Lys Cys Tyr Trp Arg Gly Asn Ala 485 490 495 Asp Glu Ala Arg Glu Glu Leu Glu Pro Leu Lys Glu Leu Arg Asn Arg 500 505 510 Cys Ile Lys Gln Leu Glu Asn Met Arg Pro Asp His Met Arg Arg Leu 515 520 525 Asn Pro Thr Pro Tyr Lys Val Ser Val Ser Ala Lys Leu Tyr Asp Phe 530 535 540 Ile His Phe Leu Trp Leu Asn Glu Ala Pro Val Gly Glu Leu His 545 550 555 <210> 19 <211> 1674 <212> DNA <213> Arabidopsis thaliana <400> 19 atggagccga aagagaacgg ctcagaattg ggtcagaaga tcattgacgg accaacgaat 60 ccaatggtca cacctttact caatgatctt tatcaattca ccatggctta tgcttattgg 120 aaagctggca aacacaacga acgatccgtt ttcgatctgt attttcgtaa gaacccattt 180 ggtggtgagt acactgtgtt tgctggatta gaagagtgtg ttaagttctt agccaatttc 240 aaattgactg atgaagaaat cgatttcgtt caagagtgtt tgcctggatc tgaggaagct 300 ttttgtgatt atcttagagg gcttgattgt tctgatgttg aagtttatgc aattccggaa 360 ggatcagttg tttttcctaa agtacctctc atgagagttg aaggacctgt tggtgttgtt 420 caattgttgg aaactccatt cctcaatctt gtcaattttg catctttggt agctactaac 480 gcagctaggc atcgctttgt tgccggaaaa tctaagagtc tactcgagtt tggtgctcga 540 agggctcagg gtccggatgg tgcaataagc gcatcaaaat attgctacct tggaggtttt 600 gatgcaacaa gtaatgtagc agctggaaaa ctttttggga ttcctcttcg tggaacacac 660 tctcatgctt atgttagctc attcatgagt actgatgaga ttgttgacaa agtacttcgt 720 agtgctgatg ggaaaaccac gtgcgaggat tttgttagtc atgttcagac atggttaaaa 780 aagattcagt attcaccatc tctaagtggc attttctctg agacaaatca aagcgagcta 840 gcagctttca cctcatatgc actggcattc cccaaaactt ttcttgccct cgtagataca 900 tacgatgtga tgaagagtgg aatccctaac ttctgtgcag ttgctttagc actcaatgac 960 tttggatata aagcattagg tattagactg gattcaggtg atttagctta tctatctaga 1020 gaggccagaa atttcttctg cacggtagag agagaactaa aagtgcctgg ttttgggaag 1080 atggtcgtca ctgctagtaa tgatctaaat gaagagacga ttgacgcttt aaataaacag 1140 ggacatgagg tggatgcttt tggcatcggg acctacttgg tcacttgcta ttcacaagcg 1200 gccttaggtt gcgttttcaa acttgtggag ataaacaatc agcctcggat taaactttct 1260 gaagatgtta caaaggtatc aataccgtgt aaaaagcgaa gttacagatt atacggcaaa 1320 gaaggttacc ctctggtaga tataatgact ggagagaacg aaccacctcc aaaggttggt 1380 gagcgtttac tttgtcgtca cccattcaac gaatccaaaa gagcatatgt agtgccacaa 1440 cgtgtcgaag agctcctcaa atgttattgg cgtggaagtg cagatgaagc aagagaagta 1500 ttaccgcctt tgaaagagat aagagaccgt tgcatcaaac agctcgaaaa catgcgacct 1560 gatcatatga ggagattaaa cccaactcct tataaggtta gtgtaagcgc aaagctgtac 1620 gatttcatcc acttcttatg gctaaacgaa gcacctgttg gtgaattgca gtga 1674 <210> 20 <211> 557 <212> PRT <213> Arabidopsis thaliana <400> 20 Met Glu Pro Lys Glu Asn Gly Ser Glu Leu Gly Gln Lys Ile Ile Asp 1 5 10 15 Gly Pro Thr Asn Pro Met Val Thr Pro Leu Leu Asn Asp Leu Tyr Gln 20 25 30 Phe Thr Met Ala Tyr Ala Tyr Trp Lys Ala Gly Lys His Asn Glu Arg 35 40 45 Ser Val Phe Asp Leu Tyr Phe Arg Lys Asn Pro Phe Gly Gly Glu Tyr 50 55 60 Thr Val Phe Ala Gly Leu Glu Glu Cys Val Lys Phe Leu Ala Asn Phe 65 70 75 80 Lys Leu Thr Asp Glu Glu Ile Asp Phe Val Gln Glu Cys Leu Pro Gly 85 90 95 Ser Glu Glu Ala Phe Cys Asp Tyr Leu Arg Gly Leu Asp Cys Ser Asp 100 105 110 Val Glu Val Tyr Ala Ile Pro Glu Gly Ser Val Val Phe Pro Lys Val 115 120 125 Pro Leu Met Arg Val Glu Gly Pro Val Gly Val Val Gln Leu Leu Glu 130 135 140 Thr Pro Phe Leu Asn Leu Val Asn Phe Ala Ser Leu Val Ala Thr Asn 145 150 155 160 Ala Ala Arg His Arg Phe Val Ala Gly Lys Ser Lys Ser Leu Leu Glu 165 170 175 Phe Gly Ala Arg Arg Ala Gln Gly Pro Asp Gly Ala Ile Ser Ala Ser 180 185 190 Lys Tyr Cys Tyr Leu Gly Gly Phe Asp Ala Thr Ser Asn Val Ala Ala 195 200 205 Gly Lys Leu Phe Gly Ile Pro Leu Arg Gly Thr His Ser His Ala Tyr 210 215 220 Val Ser Ser Phe Met Ser Thr Asp Glu Ile Val Asp Lys Val Leu Arg 225 230 235 240 Ser Ala Asp Gly Lys Thr Thr Cys Glu Asp Phe Val Ser His Val Gln 245 250 255 Thr Trp Leu Lys Lys Ile Gln Tyr Ser Pro Ser Leu Ser Gly Ile Phe 260 265 270 Ser Glu Thr Asn Gln Ser Glu Leu Ala Ala Phe Thr Ser Tyr Ala Leu 275 280 285 Ala Phe Pro Lys Thr Phe Leu Ala Leu Val Asp Thr Tyr Asp Val Met 290 295 300 Lys Ser Gly Ile Pro Asn Phe Cys Ala Val Ala Leu Ala Leu Asn Asp 305 310 315 320 Phe Gly Tyr Lys Ala Leu Gly Ile Arg Leu Asp Ser Gly Asp Leu Ala 325 330 335 Tyr Leu Ser Arg Glu Ala Arg Asn Phe Phe Cys Thr Val Glu Arg Glu 340 345 350 Leu Lys Val Pro Gly Phe Gly Lys Met Val Val Thr Ala Ser Asn Asp 355 360 365 Leu Asn Glu Glu Thr Ile Asp Ala Leu Asn Lys Gln Gly His Glu Val 370 375 380 Asp Ala Phe Gly Ile Gly Thr Tyr Leu Val Thr Cys Tyr Ser Gln Ala 385 390 395 400 Ala Leu Gly Cys Val Phe Lys Leu Val Glu Ile Asn Asn Gln Pro Arg 405 410 415 Ile Lys Leu Ser Glu Asp Val Thr Lys Val Ser Ile Pro Cys Lys Lys 420 425 430 Arg Ser Tyr Arg Leu Tyr Gly Lys Glu Gly Tyr Pro Leu Val Asp Ile 435 440 445 Met Thr Gly Glu Asn Glu Pro Pro Pro Lys Val Gly Glu Arg Leu Leu 450 455 460 Cys Arg His Pro Phe Asn Glu Ser Lys Arg Ala Tyr Val Val Pro Gln 465 470 475 480 Arg Val Glu Glu Leu Leu Lys Cys Tyr Trp Arg Gly Ser Ala Asp Glu 485 490 495 Ala Arg Glu Val Leu Pro Pro Leu Lys Glu Ile Arg Asp Arg Cys Ile 500 505 510 Lys Gln Leu Glu Asn Met Arg Pro Asp His Met Arg Arg Leu Asn Pro 515 520 525 Thr Pro Tyr Lys Val Ser Val Ser Ala Lys Leu Tyr Asp Phe Ile His 530 535 540 Phe Leu Trp Leu Asn Glu Ala Pro Val Gly Glu Leu Gln 545 550 555 <210> 21 <211> 717 <212> DNA <213> Arabidopsis thaliana <400> 21 atggatgtcc cgttaccagt cgagaaatta tcttatggat caaacactga ggacaaaact 60 tgtgtagtgc ttgtggcaac tgggagtttc aatcctccta ctttcatgca tttacgcatg 120 tttgagctgg cgagagatga attacgctca aaaggatttc atgttcttgg aggatatatg 180 tctcctgtta atgatgcata taagaagaag ggccttttat ctgcagaaca tcgtttagag 240 atgtgtaatg tatcatgtca aagctctgac tttgtaatgg ttgatccgtg ggaggcatct 300 caaagcaact accaacgaac tttgacggtt ttatcaaggg tcaagacttt cttaacaaca 360 aatcgacatg tacccgagga atctctcaaa gtcatgctac tatgtggctc ggatttactg 420 ctatctttct gcactcccgg tgtttggatc cctgaacagt taagaactat ttgcaaagat 480 tatggcattg tgtgcatccg tagagaagga caagatgttg aaaatatgat ctctggtgac 540 gaaatcttaa acgaaaactg tgctaacgtc aaaatcgttg acaatactgt tcctaatcaa 600 atcagttcga gtagattaag gcaatgcatt tcgcgagggt tatcggttaa atacttgact 660 gaagatggag taatagatta tatcagacaa catcaactat acactgagct cacatga 717 <210> 22 <211> 238 <212> PRT <213> Arabidopsis thaliana <400> 22 Met Asp Val Pro Leu Pro Val Glu Lys Leu Ser Tyr Gly Ser Asn Thr 1 5 10 15 Glu Asp Lys Thr Cys Val Val Leu Val Ala Thr Gly Ser Phe Asn Pro 20 25 30 Pro Thr Phe Met His Leu Arg Met Phe Glu Leu Ala Arg Asp Glu Leu 35 40 45 Arg Ser Lys Gly Phe His Val Leu Gly Gly Tyr Met Ser Pro Val Asn 50 55 60 Asp Ala Tyr Lys Lys Lys Gly Leu Leu Ser Ala Glu His Arg Leu Glu 65 70 75 80 Met Cys Asn Val Ser Cys Gln Ser Ser Asp Phe Val Met Val Asp Pro 85 90 95 Trp Glu Ala Ser Gln Ser Asn Tyr Gln Arg Thr Leu Thr Val Leu Ser 100 105 110 Arg Val Lys Thr Phe Leu Thr Thr Asn Arg His Val Pro Glu Glu Ser 115 120 125 Leu Lys Val Met Leu Leu Cys Gly Ser Asp Leu Leu Leu Ser Phe Cys 130 135 140 Thr Pro Gly Val Trp Ile Pro Glu Gln Leu Arg Thr Ile Cys Lys Asp 145 150 155 160 Tyr Gly Ile Val Cys Ile Arg Arg Glu Gly Gln Asp Val Glu Asn Met 165 170 175 Ile Ser Gly Asp Glu Ile Leu Asn Glu Asn Cys Ala Asn Val Lys Ile 180 185 190 Val Asp Asn Thr Val Pro Asn Gln Ile Ser Ser Ser Arg Leu Arg Gln 195 200 205 Cys Ile Ser Arg Gly Leu Ser Val Lys Tyr Leu Thr Glu Asp Gly Val 210 215 220 Ile Asp Tyr Ile Arg Gln His Gln Leu Tyr Thr Glu Leu Thr 225 230 235 <210> 23 <211> 2178 <212> DNA <213> Arabidopsis thaliana <400> 23 atgaggctgt tgaaggttgc tacgtgtaac ttgaaccaat gggccatgga tttcgagagc 60 aacatgaaga acatcaaggc ttcgatcgct gaggcaaagg ctgctggtgc tgttatcagg 120 cttggacccg agctcgaggt cactggctat ggttgcgagg atcacttctt ggaactcgac 180 actgtcactc atgcgtggga gtgtttgaag gaattgctgc ttggtgattg gacggatgat 240 attttgtgca gcataggaat gcctgtgatt aaaggagcag agcgttataa ctgccaggtt 300 ctctgtatga acagaagaat catcatgatt cgaccgaaaa tgtggctcgc aaacgatgga 360 aactataggg agctacggtg gttcacagct tggaagcaga gagaagagct agaggaattt 420 cagctcccca ttgaaatttc agaggctttg gagcagaaat cagtcccttt tggttatggt 480 tacatccagt ttatcgacac ggctgttgca gctgaagtct gtgaggaact gtttagtcca 540 cttcctcctc atgccgagct cgcattgaat ggtgttgaag tatttatgaa tgcaagtggg 600 agtcatcacc aacttaggaa actagatatt cgtctgaatg cttttatggg ggctactcat 660 gctcgtggtg gggtgtatat gtacagtaat caacaaggat gcgatggtag ccgcttatac 720 tacgatggat gtgcatgtat tgttgtaaac gggaatgttg ttgctcaagg ctcacaattc 780 tcgttgagag acgttgaggt catcatttca caagtggatc ttgatgcggt tgctagcctt 840 cgtggatcta taagtagctt tcaggaacaa gcaagctgca aggttaaagt atcttcagta 900 gctgtgccct gtagacttac acagtccttc aacctgaaaa tgacactaag cagtccgaag 960 aagatcattt accactctcc acaagaagaa atagcctttg gtcccgcttg ctggatgtgg 1020 gactatttga gaagaagtgg cgcttcagga tttttgcttc ctctttctgg cggagcagac 1080 agctcctccg tggcagctat tgttggctgc atgtgccaac ttgttgttaa agagattgca 1140 aagggagatg agcaagtaaa agctgatgcg aaccgaattg ggaattatgc taatgggcag 1200 tttcctactg atagcaaaga gtttgccaaa cgaatatttt acactgtctt tatgggttct 1260 gaaaacagtt ctgaggagac aaaaaggcgt tcaaagcagc tggcagacga gattggtgct 1320 tggcatcttg atgtttgcat agatggtgtt gtctctgcag ttttatcatt atttcaaaca 1380 gttacaggca agcgaccaag gtataaggtt gatggaggat caaatgctga gaaccttggg 1440 ttgcagaaca ttcaagcccg gatgagaatg gtgttagcat ttatgttagc gtctctcttg 1500 ccttgggttc atagcaaacc aggcttttac cttgttctag gcagctccaa cgttgatgaa 1560 ggacttcgtg gttacctgac aaagtatgat tgcagctcag cagacataaa tcctatagga 1620 agtatcagta aaatggattt gaggttgttc ttaaaatggg ctgcaacgaa tctcggatat 1680 ccatccttgg cagagataga agctgctcca ccaacagctg agcttgagcc cattcgttct 1740 gactattctc agctcgatga agtcgacatg ggaatgacat atgaagagct ttcagtctat 1800 ggaaggatga ggaagatatt ccgttgtgga ccagtatcta tgttcaagaa tctatgttac 1860 aagtggggaa caaagctaag cccagcagaa gtagctgaga aagtgaagta tttcttcaaa 1920 tattattcga tcaatcgaca caaaatgact gtcctcacac cgtcttatca cgctgagagt 1980 tactccccag aggacaacag attcgatctg aggcagtttc tgtacaacag caagtggcca 2040 taccagttta agaagattga cgagattgtt gacagcttaa atggtgactc agttgctttc 2100 ccggaagaag aagcaaactc caacaaagaa attggagttg tagcagcaaa ctccggagac 2160 ccaagtgcgg gtctctga 2178 <210> 24 <211> 725 <212> PRT <213> Arabidopsis thaliana <400> 24 Met Arg Leu Leu Lys Val Ala Thr Cys Asn Leu Asn Gln Trp Ala Met 1 5 10 15 Asp Phe Glu Ser Asn Met Lys Asn Ile Lys Ala Ser Ile Ala Glu Ala 20 25 30 Lys Ala Ala Gly Ala Val Ile Arg Leu Gly Pro Glu Leu Glu Val Thr 35 40 45 Gly Tyr Gly Cys Glu Asp His Phe Leu Glu Leu Asp Thr Val Thr His 50 55 60 Ala Trp Glu Cys Leu Lys Glu Leu Leu Leu Gly Asp Trp Thr Asp Asp 65 70 75 80 Ile Leu Cys Ser Ile Gly Met Pro Val Ile Lys Gly Ala Glu Arg Tyr 85 90 95 Asn Cys Gln Val Leu Cys Met Asn Arg Arg Ile Ile Met Ile Arg Pro 100 105 110 Lys Met Trp Leu Ala Asn Asp Gly Asn Tyr Arg Glu Leu Arg Trp Phe 115 120 125 Thr Ala Trp Lys Gln Arg Glu Glu Leu Glu Glu Phe Gln Leu Pro Ile 130 135 140 Glu Ile Ser Glu Ala Leu Glu Gln Lys Ser Val Pro Phe Gly Tyr Gly 145 150 155 160 Tyr Ile Gln Phe Ile Asp Thr Ala Val Ala Ala Glu Val Cys Glu Glu 165 170 175 Leu Phe Ser Pro Leu Pro Pro His Ala Glu Leu Ala Leu Asn Gly Val 180 185 190 Glu Val Phe Met Asn Ala Ser Gly Ser His His Gln Leu Arg Lys Leu 195 200 205 Asp Ile Arg Leu Asn Ala Phe Met Gly Ala Thr His Ala Arg Gly Gly 210 215 220 Val Tyr Met Tyr Ser Asn Gln Gln Gly Cys Asp Gly Ser Arg Leu Tyr 225 230 235 240 Tyr Asp Gly Cys Ala Cys Ile Val Val Asn Gly Asn Val Val Ala Gln 245 250 255 Gly Ser Gln Phe Ser Leu Arg Asp Val Glu Val Ile Ile Ser Gln Val 260 265 270 Asp Leu Asp Ala Val Ala Ser Leu Arg Gly Ser Ile Ser Ser Phe Gln 275 280 285 Glu Gln Ala Ser Cys Lys Val Lys Val Ser Ser Val Ala Val Pro Cys 290 295 300 Arg Leu Thr Gln Ser Phe Asn Leu Lys Met Thr Leu Ser Ser Pro Lys 305 310 315 320 Lys Ile Ile Tyr His Ser Pro Gln Glu Glu Ile Ala Phe Gly Pro Ala 325 330 335 Cys Trp Met Trp Asp Tyr Leu Arg Arg Ser Gly Ala Ser Gly Phe Leu 340 345 350 Leu Pro Leu Ser Gly Gly Ala Asp Ser Ser Ser Val Ala Ala Ile Val 355 360 365 Gly Cys Met Cys Gln Leu Val Val Lys Glu Ile Ala Lys Gly Asp Glu 370 375 380 Gln Val Lys Ala Asp Ala Asn Arg Ile Gly Asn Tyr Ala Asn Gly Gln 385 390 395 400 Phe Pro Thr Asp Ser Lys Glu Phe Ala Lys Arg Ile Phe Tyr Thr Val 405 410 415 Phe Met Gly Ser Glu Asn Ser Ser Glu Glu Thr Lys Arg Arg Ser Lys 420 425 430 Gln Leu Ala Asp Glu Ile Gly Ala Trp His Leu Asp Val Cys Ile Asp 435 440 445 Gly Val Val Ser Ala Val Leu Ser Leu Phe Gln Thr Val Thr Gly Lys 450 455 460 Arg Pro Arg Tyr Lys Val Asp Gly Gly Ser Asn Ala Glu Asn Leu Gly 465 470 475 480 Leu Gln Asn Ile Gln Ala Arg Met Arg Met Val Leu Ala Phe Met Leu 485 490 495 Ala Ser Leu Leu Pro Trp Val His Ser Lys Pro Gly Phe Tyr Leu Val 500 505 510 Leu Gly Ser Ser Asn Val Asp Glu Gly Leu Arg Gly Tyr Leu Thr Lys 515 520 525 Tyr Asp Cys Ser Ser Ala Asp Ile Asn Pro Ile Gly Ser Ile Ser Lys 530 535 540 Met Asp Leu Arg Leu Phe Leu Lys Trp Ala Ala Thr Asn Leu Gly Tyr 545 550 555 560 Pro Ser Leu Ala Glu Ile Glu Ala Ala Pro Pro Thr Ala Glu Leu Glu 565 570 575 Pro Ile Arg Ser Asp Tyr Ser Gln Leu Asp Glu Val Asp Met Gly Met 580 585 590 Thr Tyr Glu Glu Leu Ser Val Tyr Gly Arg Met Arg Lys Ile Phe Arg 595 600 605 Cys Gly Pro Val Ser Met Phe Lys Asn Leu Cys Tyr Lys Trp Gly Thr 610 615 620 Lys Leu Ser Pro Ala Glu Val Ala Glu Lys Val Lys Tyr Phe Phe Lys 625 630 635 640 Tyr Tyr Ser Ile Asn Arg His Lys Met Thr Val Leu Thr Pro Ser Tyr 645 650 655 His Ala Glu Ser Tyr Ser Pro Glu Asp Asn Arg Phe Asp Leu Arg Gln 660 665 670 Phe Leu Tyr Asn Ser Lys Trp Pro Tyr Gln Phe Lys Lys Ile Asp Glu 675 680 685 Ile Val Asp Ser Leu Asn Gly Asp Ser Val Ala Phe Pro Glu Glu Glu 690 695 700 Ala Asn Ser Asn Lys Glu Ile Gly Val Val Ala Ala Asn Ser Gly Asp 705 710 715 720 Pro Ser Ala Gly Leu 725 <210> 25 <211> 11811 <212> DNA <213> Artificial <220> <223> pTVE467 <400> 25 agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60 cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120 acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180 cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240 cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300 ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360 tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420 aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480 agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agcttattta 540 tccacgacat tgatgttgtg ggccttcaac tcttccttaa ccttattgat gacttcggga 600 tcatcgctga tgggtcttgt gtaatccagc aggacagtgg tcttataacc tagttctgca 660 gcggaaatgg cggtggcttt gacacaatac tccaaagcta caccgacaat gtaaacctcg 720 tctgtatgat gcttttctaa gtacttgttc atgtcggtct tatggaagtt ccagatgtcg 780 tggaaggcgg agtagtattc acggtcagtc aagaaaccct tgtcgacaat cttaatatgc 840 ttagtgacca cttggtccat tatttggtca accaattgac taccccaggt gtttttcaca 900 cagtgtacgg gccacaaaat accctcttgc gtggaatcat cgcctggcct tggagagtgg 960 taggtgtatg ttgaataggg ttctttatct ttatggttct ttgcgaacga aatatgtctg 1020 gaagggtgcc aatctctggt gaccacaatc ctgtgccagt ctctatcagc atcttgcatc 1080 aaatccgaga taggattgat taattcctca ccttttggaa cagtcaagga acctaaaggt 1140 gaaataaaat cattttgcat atcaacaaca attaaagtct ccatggtttt ggtttaataa 1200 gaagagaaaa gagttctttt gttatggctg aagtaataga gaaatgagct cgagtcctct 1260 ccaaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg 1320 cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg 1380 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt 1440 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc 1500 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga 1560 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga 1620 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttgacgaaga 1680 ttttcttctt gtcattgagt cgtaaaagac tctgtatgaa ctgttcgcca gtcttcacgg 1740 cgagttctgt tagatcctcg atctgaattt ttgactccat gtatggtgca tatggcgcgc 1800 catatgcccg ggccctgtac agcggccgcg ttaacgcgta tactctagag cgatcgcccg 1860 ggccggccat ttaaatgaat tcgagctcgg tacccaaacg cggccgcaag ctataacttc 1920 gtatagcata cattatacga agttattcga ctctagagga tcccaattcc catgcatgga 1980 gtcaaagatt caaatagagg acacttctcg aactcggccg tcgaactcgg ccgtcgagta 2040 catggtcgat aagaaaaggc aatttgtaga tgttaattcc catcttgaaa gaaatatagt 2100 ttaaatattt attgataaaa taacaagtca ggtattatag tccaagcaaa aacataaatt 2160 tattgatgca agtttaaatt cagaaatatt tcaataactg attatatcag ctggtacatt 2220 gccgtagatg aaagactgag tgcgatatta tgtgtaatac ataaattgat gatatagcta 2280 gcttagctca tcgggggatc ctagacgcgt gagatcagat ctcggtgacg ggcaggaccg 2340 gacggggcgg taccggcagg ctgaagtcca gctgccagaa acccacgtca tgccagttcc 2400 cgtgcttgaa gccggccgcc cgcagcatgc cgcggggggc atatccgagc gcctcgtgca 2460 tgcgcacgct cgggtcgttg ggcagcccga tgacagcgac cacgctcttg aagccctgtg 2520 cctccaggga cttcagcagg tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc 2580 ggggggagac gtacacggtc gactcggccg tccagtcgta ggcgttgcgt gccttccagg 2640 ggcccgcgta ggcgatgccg gcgacctcgc cgtccacctc ggcgacgagc cagggatagc 2700 gctcccgcag acggacgagg tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga 2760 agttgaccgt gcttgtctcg atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg 2820 cctcggtggc acggcggatg tcggccgggc gtcgttctgg gtccattgtt cttctttact 2880 ctttgtgtga ctgaggtttg gtctagtgct ttggtcatct atatataatg ataacaacaa 2940 tgagaacaag ctttggagtg atcggagggt ctaggataca tgagattcaa gtggactagg 3000 atctacaccg ttggattttg agtgtggata tgtgtgaggt taattttact tggtaacggc 3060 cacaaaggcc taaggagagg tgttgagacc cttatcggct tgaaccgctg gaataatgcc 3120 acgtggaaga taattccatg aatcttatcg ttatctatga gtgaaattgt gtgatggtgg 3180 agtggtgctt gctcatttta cttgcctggt ggacttggcc ctttccttat ggggaattta 3240 tattttactt actatagagc tttcatacct tttttttacc ttggatttag ttaatatata 3300 atggtatgat tcatgaataa aaatgggaaa tttttgaatt tgtactgcta aatgcataag 3360 attaggtgaa actgtggaat atatattttt ttcatttaaa agcaaaattt gccttttact 3420 agaattataa atatagaaaa atatataaca ttcaaataaa aatgaaaata agaactttca 3480 aaaaacagaa ctatgtttaa tgtgtaaaga ttagtcgcac atcaagtcat ctgttacaat 3540 atgttacaac aagtcataag cccaacaaag ttagcacgtc taaataaact aaagagtcca 3600 cgaaaatatt acaaatcata agcccaacaa agttattgat caaaaaaaaa aaacgcccaa 3660 caaagctaaa caaagtccaa aaaaaacttc tcaagtctcc atcttccttt atgaacattg 3720 aaaactatac acaaaacaag tcagataaat ctctttctgg gcctgtcttc ccaacctcct 3780 acatcacttc cctatcggat tgaatgtttt acttgtacct tttccgttgc aatgatattg 3840 atagtatgtt tgtgaaaact aatagggtta acaatcgaag tcatggaata tggatttggt 3900 ccaagatttt ccgagagctt tctagtagaa agcccatcac cagaaattta ctagtaaaat 3960 aaatcaccaa ttaggtttct tattatgtgc caaattcaat ataattatag aggatatttc 4020 aaatgaaaac gtatgaatgt tattagtaaa tggtcaggta agacattaaa aaaatcctac 4080 gtcagatatt caactttaaa aattcgatca gtgtggaatt gtacaaaaat ttgggatcta 4140 ctatatatat ataatgcttt acaacacttg gatttttttt tggaggctgg aatttttaat 4200 ctacatattt gttttggcca tgcaccaact cattgtttag tgtaatactt tgattttgtc 4260 aaatatatgt gttcgtgtat atttgtataa gaatttcttt gaccatatac acacacacat 4320 atatatatat atatatatat tatatatcat gcacttttaa ttgaaaaaat aatatatata 4380 tatatagtgc attttttcta acaaccatat atgttgcgat tgatctgcaa aaatactgct 4440 agagtaatga aaaatataat ctattgctga aattatctca gatgttaaga ttttcttaaa 4500 gtaaattctt tcaaatttta gctaaaagtc ttgtaataac taaagaataa tacacaatct 4560 cgaccacgga aaaaaaacac ataataaatt tgaatttcga ccgcggtacc cggaattggg 4620 ttataattac ctcaggtcga ggaattaatt cggtacgtac ctaataactt cgtatagcat 4680 acattatacg aagttatatg gatctcgagg cattacggca ttacggcact cgcgagggtc 4740 ccaattcgag catggagcca tttacaattg aatatatcct gccgccgctg ccgctttgca 4800 cccggtggag cttgcatgtt ggtttctacg cagaactgag ccggttaggc agataatttc 4860 cattgagaac tgagccatgt gcaccttccc cccaacacgg tgagcgacgg ggcaacggag 4920 tgatccacat gggactttta aacatcatcc gtcggatggc gttgcgagag aagcagtcga 4980 tccgtgagat cagccgacgc accgggcagg cgcgcaacac gatcgcaaag tatttgaacg 5040 caggtacaat cgagccgacg ttcacggtac cggaacgacc aagcaagcta gcttagtaaa 5100 gccctcgcta gattttaatg cggatgttgc gattacttcg ccaactattg cgataacaag 5160 aaaaagccag cctttcatga tatatctccc aatttgtgta gggcttatta tgcacgctta 5220 aaaataataa aagcagactt gacctgatag tttggctgtg agcaattatg tgcttagtgc 5280 atctaacgct tgagttaagc cgcgccgcga agcggcgtcg gcttgaacga attgttagac 5340 attatttgcc gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact 5400 gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa 5460 gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct 5520 tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat 5580 ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg 5640 cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca 5700 aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg 5760 ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc 5820 gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag 5880 cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag 5940 ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt 6000 gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt 6060 cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca 6120 ccgcttccct catgatgttt aactttgttt tagggcgact gccctgctgc gtaacatcgt 6180 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc 6240 gaggcataga ctgtacccca aaaaaacagt cataacaagc catgaaaacc gccactgcgc 6300 cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact 6360 tgcattacag cttacgaacc gaacaggctt atgtccactg ggttcgtgcc ttcatccgtt 6420 tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca tttctgtcct 6480 ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg tcaggcattg gcggccttgc 6540 tgttcttcta cggcaagtgc tgtgcacgga tctgccctgg cttcaggaga tcggaagacc 6600 tcggccgtcc gggcgcttgc cggtggtgct gaccccggat gaagtctcta gagctctaga 6660 gggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag cttctgtatg 6720 gaacgggcat gcggatcagt gagggtttgc aactgcgggt caaggatctg gatttcgatc 6780 acggcacgat catcgtgcgg gagggcaagg gctccaagga tcgggccttg atgttacccg 6840 agagcttggc acccagcctg cgcgagcagg gatcgatcca acccctccgc tgctatagtg 6900 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct 6960 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta 7020 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg 7080 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc 7140 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca 7200 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg 7260 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg 7320 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca 7380 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt 7440 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt 7500 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc 7560 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt 7620 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct 7680 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag 7740 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga 7800 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac 7860 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc 7920 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta 7980 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg 8040 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg 8100 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc 8160 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta 8220 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc 8280 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag 8340 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc 8400 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc 8460 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg 8520 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc 8580 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag 8640 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga 8700 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg 8760 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc 8820 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg 8880 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt 8940 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac 9000 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt 9060 gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgacggtcg 9120 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt 9180 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc 9240 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc 9300 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct 9360 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc 9420 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga 9480 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc 9540 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg 9600 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg 9660 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat 9720 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt 9780 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga 9840 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt 9900 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg 9960 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg 10020 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga 10080 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg 10140 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct 10200 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac 10260 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa 10320 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga 10380 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc 10440 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct 10500 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat 10560 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga 10620 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc 10680 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 10740 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc 10800 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt 10860 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 10920 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 10980 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 11040 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 11100 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 11160 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 11220 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 11280 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 11340 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 11400 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 11460 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 11520 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 11580 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 11640 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 11700 agaagatccg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg 11760 gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc c 11811 <210> 26 <211> 11829 <212> DNA <213> Artificial <220> <223> pTVE468 <400> 26 agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60 cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120 acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180 cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240 cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300 ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360 tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420 aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480 agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agcttattta 540 tccacgacat tgatgttgtg ggccttcaac tcttccttaa ccttattgat gacttcggga 600 tcatcgctga tgggtcttgt gtaatccagc aggacagtgg tcttataacc tagttctgca 660 gcggaaatgg cggtggcttt gacacaatac tccaaagcta caccgacaat gtaaacctcg 720 tctgtatgat gcttttctaa gtacttgttc atgtcggtct tatggaagtt ccagatgtcg 780 tggaaggcgg agtagtattc acggtcagtc aagaaaccct tgtcgacaat cttaatatgc 840 ttagtgacca cttggtccat tatttggtca accaattgac taccccaggt gtttttcaca 900 cagtgtacgg gccacaaaat accctcttgc gtggaatcat cgcctggcct tggagagtgg 960 taggtgtatg ttgaataggg ttctttatct ttatggttct ttgcgaacga aatatgtctg 1020 gaagggtgcc aatctctggt gaccacaatc ctgtgccagt ctctatcagc atcttgcatc 1080 aaatccgaga taggattgat taattcctca ccttttggaa cagtcaagga acctaaaggt 1140 gaaataaaat cattttgcat atcaacaacc ttgcgcttct tcttgggaat taaagtctcc 1200 atggttttgg tttaataaga agagaaaaga gttcttttgt tatggctgaa gtaatagaga 1260 aatgagctcg agtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg 1320 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt 1380 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc 1440 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat 1500 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag 1560 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag 1620 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct 1680 ccaccatgtt gacgaagatt ttcttcttgt cattgagtcg taaaagactc tgtatgaact 1740 gttcgccagt cttcacggcg agttctgtta gatcctcgat ctgaattttt gactccatgt 1800 atggtgcata tggcgcgcca tatgcccggg ccctgtacag cggccgcgtt aacgcgtata 1860 ctctagagcg atcgcccggg ccggccattt aaatgaattc gagctcggta cccaaacgcg 1920 gccgcaagct ataacttcgt atagcataca ttatacgaag ttattcgact ctagaggatc 1980 ccaattccca tgcatggagt caaagattca aatagaggac acttctcgaa ctcggccgtc 2040 gaactcggcc gtcgagtaca tggtcgataa gaaaaggcaa tttgtagatg ttaattccca 2100 tcttgaaaga aatatagttt aaatatttat tgataaaata acaagtcagg tattatagtc 2160 caagcaaaaa cataaattta ttgatgcaag tttaaattca gaaatatttc aataactgat 2220 tatatcagct ggtacattgc cgtagatgaa agactgagtg cgatattatg tgtaatacat 2280 aaattgatga tatagctagc ttagctcatc gggggatcct agacgcgtga gatcagatct 2340 cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc tgccagaaac 2400 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat 2460 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca 2520 cgctcttgaa gccctgtgcc tccagggact tcagcaggtg ggtgtagagc gtggagccca 2580 gtcccgtccg ctggtggcgg ggggagacgt acacggtcga ctcggccgtc cagtcgtagg 2640 cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc gacctcgccg tccacctcgg 2700 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt 2760 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc 2820 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggt 2880 ccattgttct tctttactct ttgtgtgact gaggtttggt ctagtgcttt ggtcatctat 2940 atataatgat aacaacaatg agaacaagct ttggagtgat cggagggtct aggatacatg 3000 agattcaagt ggactaggat ctacaccgtt ggattttgag tgtggatatg tgtgaggtta 3060 attttacttg gtaacggcca caaaggccta aggagaggtg ttgagaccct tatcggcttg 3120 aaccgctgga ataatgccac gtggaagata attccatgaa tcttatcgtt atctatgagt 3180 gaaattgtgt gatggtggag tggtgcttgc tcattttact tgcctggtgg acttggccct 3240 ttccttatgg ggaatttata ttttacttac tatagagctt tcataccttt tttttacctt 3300 ggatttagtt aatatataat ggtatgattc atgaataaaa atgggaaatt tttgaatttg 3360 tactgctaaa tgcataagat taggtgaaac tgtggaatat atattttttt catttaaaag 3420 caaaatttgc cttttactag aattataaat atagaaaaat atataacatt caaataaaaa 3480 tgaaaataag aactttcaaa aaacagaact atgtttaatg tgtaaagatt agtcgcacat 3540 caagtcatct gttacaatat gttacaacaa gtcataagcc caacaaagtt agcacgtcta 3600 aataaactaa agagtccacg aaaatattac aaatcataag cccaacaaag ttattgatca 3660 aaaaaaaaaa acgcccaaca aagctaaaca aagtccaaaa aaaacttctc aagtctccat 3720 cttcctttat gaacattgaa aactatacac aaaacaagtc agataaatct ctttctgggc 3780 ctgtcttccc aacctcctac atcacttccc tatcggattg aatgttttac ttgtaccttt 3840 tccgttgcaa tgatattgat agtatgtttg tgaaaactaa tagggttaac aatcgaagtc 3900 atggaatatg gatttggtcc aagattttcc gagagctttc tagtagaaag cccatcacca 3960 gaaatttact agtaaaataa atcaccaatt aggtttctta ttatgtgcca aattcaatat 4020 aattatagag gatatttcaa atgaaaacgt atgaatgtta ttagtaaatg gtcaggtaag 4080 acattaaaaa aatcctacgt cagatattca actttaaaaa ttcgatcagt gtggaattgt 4140 acaaaaattt gggatctact atatatatat aatgctttac aacacttgga tttttttttg 4200 gaggctggaa tttttaatct acatatttgt tttggccatg caccaactca ttgtttagtg 4260 taatactttg attttgtcaa atatatgtgt tcgtgtatat ttgtataaga atttctttga 4320 ccatatacac acacacatat atatatatat atatatatta tatatcatgc acttttaatt 4380 gaaaaaataa tatatatata tatagtgcat tttttctaac aaccatatat gttgcgattg 4440 atctgcaaaa atactgctag agtaatgaaa aatataatct attgctgaaa ttatctcaga 4500 tgttaagatt ttcttaaagt aaattctttc aaattttagc taaaagtctt gtaataacta 4560 aagaataata cacaatctcg accacggaaa aaaaacacat aataaatttg aatttcgacc 4620 gcggtacccg gaattgggtt ataattacct caggtcgagg aattaattcg gtacgtacct 4680 aataacttcg tatagcatac attatacgaa gttatatgga tctcgaggca ttacggcatt 4740 acggcactcg cgagggtccc aattcgagca tggagccatt tacaattgaa tatatcctgc 4800 cgccgctgcc gctttgcacc cggtggagct tgcatgttgg tttctacgca gaactgagcc 4860 ggttaggcag ataatttcca ttgagaactg agccatgtgc accttccccc caacacggtg 4920 agcgacgggg caacggagtg atccacatgg gacttttaaa catcatccgt cggatggcgt 4980 tgcgagagaa gcagtcgatc cgtgagatca gccgacgcac cgggcaggcg cgcaacacga 5040 tcgcaaagta tttgaacgca ggtacaatcg agccgacgtt cacggtaccg gaacgaccaa 5100 gcaagctagc ttagtaaagc cctcgctaga ttttaatgcg gatgttgcga ttacttcgcc 5160 aactattgcg ataacaagaa aaagccagcc tttcatgata tatctcccaa tttgtgtagg 5220 gcttattatg cacgcttaaa aataataaaa gcagacttga cctgatagtt tggctgtgag 5280 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc 5340 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt 5400 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat 5460 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc 5520 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg 5580 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg 5640 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct 5700 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca 5760 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt 5820 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa 5880 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca 5940 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca 6000 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta 6060 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag 6120 tcgatacttc ggcgatcacc gcttccctca tgatgtttaa ctttgtttta gggcgactgc 6180 cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg 6240 cttgctgctt ggatgcccga ggcatagact gtaccccaaa aaaacagtca taacaagcca 6300 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc 6360 gtgagcgcat acgctacttg cattacagct tacgaaccga acaggcttat gtccactggg 6420 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag 6480 tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc 6540 aggcattggc ggccttgctg ttcttctacg gcaagtgctg tgcacggatc tgccctggct 6600 tcaggagatc ggaagacctc ggccgtccgg gcgcttgccg gtggtgctga ccccggatga 6660 agtctctaga gctctagagg gttcgcatcc tcggttttct ggaaggcgag catcgtttgt 6720 tcgcccagct tctgtatgga acgggcatgc ggatcagtga gggtttgcaa ctgcgggtca 6780 aggatctgga tttcgatcac ggcacgatca tcgtgcggga gggcaagggc tccaaggatc 6840 gggccttgat gttacccgag agcttggcac ccagcctgcg cgagcaggga tcgatccaac 6900 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg 6960 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt 7020 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt 7080 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg 7140 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt 7200 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc 7260 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg 7320 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag 7380 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca 7440 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca 7500 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg 7560 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg 7620 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg 7680 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg 7740 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc 7800 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc 7860 gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa 7920 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag 7980 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg 8040 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa 8100 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg 8160 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg 8220 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg 8280 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg 8340 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac 8400 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat 8460 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt 8520 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag 8580 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag 8640 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga 8700 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga 8760 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc 8820 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc 8880 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga 8940 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa 9000 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca 9060 ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag 9120 gaatcggcgt gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga 9180 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga 9240 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca 9300 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga 9360 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt 9420 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct 9480 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta 9540 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg 9600 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg 9660 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa 9720 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt 9780 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc 9840 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa 9900 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt 9960 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac 10020 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa 10080 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg 10140 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta 10200 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct 10260 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc 10320 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat 10380 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa 10440 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc 10500 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc 10560 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc 10620 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg 10680 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 10740 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 10800 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc 10860 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 10920 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg 10980 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 11040 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 11100 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 11160 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 11220 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 11280 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 11340 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 11400 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 11460 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 11520 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 11580 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 11640 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 11700 cagaaaaaaa ggatctcaag aagatccgga aaacgcaagc gcaaagagaa agcaggtagc 11760 ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc 11820 ggaattgcc 11829 <210> 27 <211> 12393 <212> DNA <213> Artificial <220> <223> pTVE469 <400> 27 agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60 cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120 acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180 cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240 cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300 ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360 tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420 aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480 agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agcttatgct 540 atagggaggc ataattgatg cttggcttga ataacagcat cacgatcacc agtatttttc 600 atgatatcgt cactaatctt cactgcttta gttccttcag cggaaaaaag cttaataaca 660 atattcatag gtttgctcac ttcactggga ttggaaactt tttggaagtc gctggtaaga 720 tttgtgccga tgccaaatgc agacttaatg ccacatttct cgcagtattt gtacagttcg 780 atacatctgt caacatttaa agcatcgcta tgaacaatta cttttgtgga aggatcgaca 840 cctattgatt tatagtgctt tacgactttt tcaatgtatt cctcagcaca accgctatct 900 tgacgaacac catggaaaac attggctaaa tcgtcggcag aattggctgt aaaagatttg 960 agaaacacat cagtagagaa tgtatccgtt aaggctatta aaagactagt accaaaagtt 1020 tggacccact ttaaggaagc aatacgattt gcttgtttat aattttgagt aatagctgca 1080 atgcccatat accactcgtg agcaaccgta ccagagacat ttagattata tttggcggcg 1140 aagtaaacat tagatgtacc aaggaaactt ccagggccct taaaatcctc ttgtgctttc 1200 atgagacctt ggagaacaat ttcctgggtg tgaggatcac gacgacgacg agtgccaaag 1260 tcagtaaagg cacatccggc tcggatgaga cgcttaccct tctcgtaagc tttttcaaac 1320 tgaccctcag gtgaccagtc cttatcgaca aatttaaaat aagattctga gacgagagca 1380 agcagtggaa tttcataaaa aatggtattc ttccagaggc cgtgaataaa gattgagaga 1440 tccttagttt cagaatcata attaagggaa attgaatttt caggatcaaa ttcgaactca 1500 tgcatgaatt cataaaatga ttcctttaaa taaggacagt tcttgcgaag ccattgctct 1560 tcttcaggaa gtaaatgtaa attccgtaag cctcttattt gttcccgtaa ccagttataa 1620 gcctcctgat ttaatgccat ttttggggac cggtttgtat acttatatga tacttgagca 1680 tccggataat gctctaaaac ggcttgaagc atggtgagtt tgtaaagatc cgtatcgagg 1740 atagagacaa cagccggttc acccatggtt ttggtttaat aagaagagaa aagagttctt 1800 ttgttatggc tgaagtaata gagaaatgag ctcgagtcct ctccaaatga aatgaacttc 1860 cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag 1920 tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca 1980 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa 2040 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg 2100 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta 2160 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct 2220 tggagtagac gagagtgtcg tgctccacca tgttgacgaa gattttcttc ttgtcattga 2280 gtcgtaaaag actctgtatg aactgttcgc cagtcttcac ggcgagttct gttagatcct 2340 cgatctgaat ttttgactcc atgtatggtg catatggcgc gccatatgcc cgggccctgt 2400 acagcggccg cgttaacgcg tatactctag agcgatcgcc cgggccggcc atttaaatga 2460 attcgagctc ggtacccaaa cgcggccgca agctataact tcgtatagca tacattatac 2520 gaagttattc gactctagag gatcccaatt cccatgcatg gagtcaaaga ttcaaataga 2580 ggacacttct cgaactcggc cgtcgaactc ggccgtcgag tacatggtcg ataagaaaag 2640 gcaatttgta gatgttaatt cccatcttga aagaaatata gtttaaatat ttattgataa 2700 aataacaagt caggtattat agtccaagca aaaacataaa tttattgatg caagtttaaa 2760 ttcagaaata tttcaataac tgattatatc agctggtaca ttgccgtaga tgaaagactg 2820 agtgcgatat tatgtgtaat acataaattg atgatatagc tagcttagct catcggggga 2880 tcctagacgc gtgagatcag atctcggtga cgggcaggac cggacggggc ggtaccggca 2940 ggctgaagtc cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg 3000 cccgcagcat gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt 3060 tgggcagccc gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca 3120 ggtgggtgta gagcgtggag cccagtcccg tccgctggtg gcggggggag acgtacacgg 3180 tcgactcggc cgtccagtcg taggcgttgc gtgccttcca ggggcccgcg taggcgatgc 3240 cggcgacctc gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga 3300 ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct 3360 cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga 3420 tgtcggccgg gcgtcgttct gggtccattg ttcttcttta ctctttgtgt gactgaggtt 3480 tggtctagtg ctttggtcat ctatatataa tgataacaac aatgagaaca agctttggag 3540 tgatcggagg gtctaggata catgagattc aagtggacta ggatctacac cgttggattt 3600 tgagtgtgga tatgtgtgag gttaatttta cttggtaacg gccacaaagg cctaaggaga 3660 ggtgttgaga cccttatcgg cttgaaccgc tggaataatg ccacgtggaa gataattcca 3720 tgaatcttat cgttatctat gagtgaaatt gtgtgatggt ggagtggtgc ttgctcattt 3780 tacttgcctg gtggacttgg ccctttcctt atggggaatt tatattttac ttactataga 3840 gctttcatac ctttttttta ccttggattt agttaatata taatggtatg attcatgaat 3900 aaaaatggga aatttttgaa tttgtactgc taaatgcata agattaggtg aaactgtgga 3960 atatatattt ttttcattta aaagcaaaat ttgcctttta ctagaattat aaatatagaa 4020 aaatatataa cattcaaata aaaatgaaaa taagaacttt caaaaaacag aactatgttt 4080 aatgtgtaaa gattagtcgc acatcaagtc atctgttaca atatgttaca acaagtcata 4140 agcccaacaa agttagcacg tctaaataaa ctaaagagtc cacgaaaata ttacaaatca 4200 taagcccaac aaagttattg atcaaaaaaa aaaaacgccc aacaaagcta aacaaagtcc 4260 aaaaaaaact tctcaagtct ccatcttcct ttatgaacat tgaaaactat acacaaaaca 4320 agtcagataa atctctttct gggcctgtct tcccaacctc ctacatcact tccctatcgg 4380 attgaatgtt ttacttgtac cttttccgtt gcaatgatat tgatagtatg tttgtgaaaa 4440 ctaatagggt taacaatcga agtcatggaa tatggatttg gtccaagatt ttccgagagc 4500 tttctagtag aaagcccatc accagaaatt tactagtaaa ataaatcacc aattaggttt 4560 cttattatgt gccaaattca atataattat agaggatatt tcaaatgaaa acgtatgaat 4620 gttattagta aatggtcagg taagacatta aaaaaatcct acgtcagata ttcaacttta 4680 aaaattcgat cagtgtggaa ttgtacaaaa atttgggatc tactatatat atataatgct 4740 ttacaacact tggatttttt tttggaggct ggaattttta atctacatat ttgttttggc 4800 catgcaccaa ctcattgttt agtgtaatac tttgattttg tcaaatatat gtgttcgtgt 4860 atatttgtat aagaatttct ttgaccatat acacacacac atatatatat atatatatat 4920 attatatatc atgcactttt aattgaaaaa ataatatata tatatatagt gcattttttc 4980 taacaaccat atatgttgcg attgatctgc aaaaatactg ctagagtaat gaaaaatata 5040 atctattgct gaaattatct cagatgttaa gattttctta aagtaaattc tttcaaattt 5100 tagctaaaag tcttgtaata actaaagaat aatacacaat ctcgaccacg gaaaaaaaac 5160 acataataaa tttgaatttc gaccgcggta cccggaattg ggttataatt acctcaggtc 5220 gaggaattaa ttcggtacgt acctaataac ttcgtatagc atacattata cgaagttata 5280 tggatctcga ggcattacgg cattacggca ctcgcgaggg tcccaattcg agcatggagc 5340 catttacaat tgaatatatc ctgccgccgc tgccgctttg cacccggtgg agcttgcatg 5400 ttggtttcta cgcagaactg agccggttag gcagataatt tccattgaga actgagccat 5460 gtgcaccttc cccccaacac ggtgagcgac ggggcaacgg agtgatccac atgggacttt 5520 taaacatcat ccgtcggatg gcgttgcgag agaagcagtc gatccgtgag atcagccgac 5580 gcaccgggca ggcgcgcaac acgatcgcaa agtatttgaa cgcaggtaca atcgagccga 5640 cgttcacggt accggaacga ccaagcaagc tagcttagta aagccctcgc tagattttaa 5700 tgcggatgtt gcgattactt cgccaactat tgcgataaca agaaaaagcc agcctttcat 5760 gatatatctc ccaatttgtg tagggcttat tatgcacgct taaaaataat aaaagcagac 5820 ttgacctgat agtttggctg tgagcaatta tgtgcttagt gcatctaacg cttgagttaa 5880 gccgcgccgc gaagcggcgt cggcttgaac gaattgttag acattatttg ccgactacct 5940 tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca 6000 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact 6060 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg 6120 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc 6180 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt 6240 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc 6300 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg 6360 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc 6420 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct 6480 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat 6540 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat 6600 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga 6660 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt 6720 ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca 6780 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc 6840 caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt 6900 cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa 6960 ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc 7020 cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca 7080 aggtttcggt ctccacgcat cgtcaggcat tggcggcctt gctgttcttc tacggcaagt 7140 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt ccgggcgctt 7200 gccggtggtg ctgaccccgg atgaagtctc tagagctcta gagggttcgc atcctcggtt 7260 ttctggaagg cgagcatcgt ttgttcgccc agcttctgta tggaacgggc atgcggatca 7320 gtgagggttt gcaactgcgg gtcaaggatc tggatttcga tcacggcacg atcatcgtgc 7380 gggagggcaa gggctccaag gatcgggcct tgatgttacc cgagagcttg gcacccagcc 7440 tgcgcgagca gggatcgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt 7500 cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg 7560 ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata 7620 cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg 7680 gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg 7740 cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg 7800 agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag 7860 accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg 7920 gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca 7980 tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca 8040 cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc 8100 tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga 8160 aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca 8220 gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga 8280 ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc 8340 gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat 8400 cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat 8460 cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga 8520 aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc 8580 ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag 8640 gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta 8700 gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc 8760 agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac 8820 cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac 8880 ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg 8940 attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt 9000 aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg 9060 atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc 9120 attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca 9180 accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct 9240 gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa 9300 acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc 9360 tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca 9420 agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca 9480 tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct 9540 aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg 9600 tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat 9660 ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt cgcaaaccat ccggcccggt 9720 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc 9780 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga 9840 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc 9900 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg 9960 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc 10020 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc 10080 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga 10140 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca 10200 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct 10260 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc 10320 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat 10380 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta 10440 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt 10500 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc 10560 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa 10620 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt 10680 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga 10740 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc 10800 aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa 10860 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg 10920 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc 10980 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg 11040 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc 11100 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg 11160 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca 11220 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 11280 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 11340 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg 11400 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 11460 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 11520 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 11580 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 11640 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 11700 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 11760 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 11820 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 11880 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 11940 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 12000 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 12060 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 12120 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 12180 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 12240 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc cggaaaacgc 12300 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc 12360 ggttttatgg acagcaagcg aaccggaatt gcc 12393 <210> 28 <211> 12414 <212> DNA <213> Artificial <220> <223> pTVE 470 <400> 28 agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60 cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120 acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180 cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240 cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300 ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360 tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420 aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480 agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agcttatgct 540 atagggaggc ataattgatg cttggcttga ataacagcat cacgatcacc agtatttttc 600 atgatatcgt cactaatctt cactgcttta gttccttcag cggaaaaaag cttaataaca 660 atattcatag gtttgctcac ttcactggga ttggaaactt tttggaagtc gctggtaaga 720 tttgtgccga tgccaaatgc agacttaatg ccacatttct cgcagtattt gtacagttcg 780 atacatctgt caacatttaa agcatcgcta tgaacaatta cttttgtgga aggatcgaca 840 cctattgatt tatagtgctt tacgactttt tcaatgtatt cctcagcaca accgctatct 900 tgacgaacac catggaaaac attggctaaa tcgtcggcag aattggctgt aaaagatttg 960 agaaacacat cagtagagaa tgtatccgtt aaggctatta aaagactagt accaaaagtt 1020 tggacccact ttaaggaagc aatacgattt gcttgtttat aattttgagt aatagctgca 1080 atgcccatat accactcgtg agcaaccgta ccagagacat ttagattata tttggcggcg 1140 aagtaaacat tagatgtacc aaggaaactt ccagggccct taaaatcctc ttgtgctttc 1200 atgagacctt ggagaacaat ttcctgggtg tgaggatcac gacgacgacg agtgccaaag 1260 tcagtaaagg cacatccggc tcggatgaga cgcttaccct tctcgtaagc tttttcaaac 1320 tgaccctcag gtgaccagtc cttatcgaca aatttaaaat aagattctga gacgagagca 1380 agcagtggaa tttcataaaa aatggtattc ttccagaggc cgtgaataaa gattgagaga 1440 tccttagttt cagaatcata attaagggaa attgaatttt caggatcaaa ttcgaactca 1500 tgcatgaatt cataaaatga ttcctttaaa taaggacagt tcttgcgaag ccattgctct 1560 tcttcaggaa gtaaatgtaa attccgtaag cctcttattt gttcccgtaa ccagttataa 1620 gcctcctgat ttaatgccat ttttggggac cggtttgtat acttatatga tacttgagca 1680 tccggataat gctctaaaac ggcttgaagc atggtgagtt tgtaaagatc cgtatcgagg 1740 atagagacaa cagcaacctt gcgcttcttc ttgggcggtt cacccatggt tttggtttaa 1800 taagaagaga aaagagttct tttgttatgg ctgaagtaat agagaaatga gctcgagtcc 1860 tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt 1920 gtgcgtcatc ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg 1980 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac 2040 tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg 2100 tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc 2160 cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg 2220 agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttgacga 2280 agattttctt cttgtcattg agtcgtaaaa gactctgtat gaactgttcg ccagtcttca 2340 cggcgagttc tgttagatcc tcgatctgaa tttttgactc catgtatggt gcatatggcg 2400 cgccatatgc ccgggccctg tacagcggcc gcgttaacgc gtatactcta gagcgatcgc 2460 ccgggccggc catttaaatg aattcgagct cggtacccaa acgcggccgc aagctataac 2520 ttcgtatagc atacattata cgaagttatt cgactctaga ggatcccaat tcccatgcat 2580 ggagtcaaag attcaaatag aggacacttc tcgaactcgg ccgtcgaact cggccgtcga 2640 gtacatggtc gataagaaaa ggcaatttgt agatgttaat tcccatcttg aaagaaatat 2700 agtttaaata tttattgata aaataacaag tcaggtatta tagtccaagc aaaaacataa 2760 atttattgat gcaagtttaa attcagaaat atttcaataa ctgattatat cagctggtac 2820 attgccgtag atgaaagact gagtgcgata ttatgtgtaa tacataaatt gatgatatag 2880 ctagcttagc tcatcggggg atcctagacg cgtgagatca gatctcggtg acgggcagga 2940 ccggacgggg cggtaccggc aggctgaagt ccagctgcca gaaacccacg tcatgccagt 3000 tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg ggcatatccg agcgcctcgt 3060 gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc ttgaagccct 3120 gtgcctccag ggacttcagc aggtgggtgt agagcgtgga gcccagtccc gtccgctggt 3180 ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg cgtgccttcc 3240 aggggcccgc gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg agccagggat 3300 agcgctcccg cagacggacg aggtcgtccg tccactcctg cggttcctgc ggctcggtac 3360 ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc gccggcatgt 3420 ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc tgggtccatt gttcttcttt 3480 actctttgtg tgactgaggt ttggtctagt gctttggtca tctatatata atgataacaa 3540 caatgagaac aagctttgga gtgatcggag ggtctaggat acatgagatt caagtggact 3600 aggatctaca ccgttggatt ttgagtgtgg atatgtgtga ggttaatttt acttggtaac 3660 ggccacaaag gcctaaggag aggtgttgag acccttatcg gcttgaaccg ctggaataat 3720 gccacgtgga agataattcc atgaatctta tcgttatcta tgagtgaaat tgtgtgatgg 3780 tggagtggtg cttgctcatt ttacttgcct ggtggacttg gccctttcct tatggggaat 3840 ttatatttta cttactatag agctttcata cctttttttt accttggatt tagttaatat 3900 ataatggtat gattcatgaa taaaaatggg aaatttttga atttgtactg ctaaatgcat 3960 aagattaggt gaaactgtgg aatatatatt tttttcattt aaaagcaaaa tttgcctttt 4020 actagaatta taaatataga aaaatatata acattcaaat aaaaatgaaa ataagaactt 4080 tcaaaaaaca gaactatgtt taatgtgtaa agattagtcg cacatcaagt catctgttac 4140 aatatgttac aacaagtcat aagcccaaca aagttagcac gtctaaataa actaaagagt 4200 ccacgaaaat attacaaatc ataagcccaa caaagttatt gatcaaaaaa aaaaaacgcc 4260 caacaaagct aaacaaagtc caaaaaaaac ttctcaagtc tccatcttcc tttatgaaca 4320 ttgaaaacta tacacaaaac aagtcagata aatctctttc tgggcctgtc ttcccaacct 4380 cctacatcac ttccctatcg gattgaatgt tttacttgta ccttttccgt tgcaatgata 4440 ttgatagtat gtttgtgaaa actaataggg ttaacaatcg aagtcatgga atatggattt 4500 ggtccaagat tttccgagag ctttctagta gaaagcccat caccagaaat ttactagtaa 4560 aataaatcac caattaggtt tcttattatg tgccaaattc aatataatta tagaggatat 4620 ttcaaatgaa aacgtatgaa tgttattagt aaatggtcag gtaagacatt aaaaaaatcc 4680 tacgtcagat attcaacttt aaaaattcga tcagtgtgga attgtacaaa aatttgggat 4740 ctactatata tatataatgc tttacaacac ttggattttt ttttggaggc tggaattttt 4800 aatctacata tttgttttgg ccatgcacca actcattgtt tagtgtaata ctttgatttt 4860 gtcaaatata tgtgttcgtg tatatttgta taagaatttc tttgaccata tacacacaca 4920 catatatata tatatatata tattatatat catgcacttt taattgaaaa aataatatat 4980 atatatatag tgcatttttt ctaacaacca tatatgttgc gattgatctg caaaaatact 5040 gctagagtaa tgaaaaatat aatctattgc tgaaattatc tcagatgtta agattttctt 5100 aaagtaaatt ctttcaaatt ttagctaaaa gtcttgtaat aactaaagaa taatacacaa 5160 tctcgaccac ggaaaaaaaa cacataataa atttgaattt cgaccgcggt acccggaatt 5220 gggttataat tacctcaggt cgaggaatta attcggtacg tacctaataa cttcgtatag 5280 catacattat acgaagttat atggatctcg aggcattacg gcattacggc actcgcgagg 5340 gtcccaattc gagcatggag ccatttacaa ttgaatatat cctgccgccg ctgccgcttt 5400 gcacccggtg gagcttgcat gttggtttct acgcagaact gagccggtta ggcagataat 5460 ttccattgag aactgagcca tgtgcacctt ccccccaaca cggtgagcga cggggcaacg 5520 gagtgatcca catgggactt ttaaacatca tccgtcggat ggcgttgcga gagaagcagt 5580 cgatccgtga gatcagccga cgcaccgggc aggcgcgcaa cacgatcgca aagtatttga 5640 acgcaggtac aatcgagccg acgttcacgg taccggaacg accaagcaag ctagcttagt 5700 aaagccctcg ctagatttta atgcggatgt tgcgattact tcgccaacta ttgcgataac 5760 aagaaaaagc cagcctttca tgatatatct cccaatttgt gtagggctta ttatgcacgc 5820 ttaaaaataa taaaagcaga cttgacctga tagtttggct gtgagcaatt atgtgcttag 5880 tgcatctaac gcttgagtta agccgcgccg cgaagcggcg tcggcttgaa cgaattgtta 5940 gacattattt gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca 6000 actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt 6060 caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat 6120 ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta 6180 catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta 6240 gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta 6300 ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg 6360 tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt 6420 cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta 6480 cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca 6540 aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac 6600 tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg 6660 gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga 6720 tcaccgcttc cctcatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat 6780 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg 6840 cccgaggcat agactgtacc ccaaaaaaac agtcataaca agccatgaaa accgccactg 6900 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct 6960 acttgcatta cagcttacga accgaacagg cttatgtcca ctgggttcgt gccttcatcc 7020 gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag gcatttctgt 7080 cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca ttggcggcct 7140 tgctgttctt ctacggcaag tgctgtgcac ggatctgccc tggcttcagg agatcggaag 7200 acctcggccg tccgggcgct tgccggtggt gctgaccccg gatgaagtct ctagagctct 7260 agagggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc cagcttctgt 7320 atggaacggg catgcggatc agtgagggtt tgcaactgcg ggtcaaggat ctggatttcg 7380 atcacggcac gatcatcgtg cgggagggca agggctccaa ggatcgggcc ttgatgttac 7440 ccgagagctt ggcacccagc ctgcgcgagc agggatcgat ccaacccctc cgctgctata 7500 gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt 7560 cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt 7620 ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga 7680 gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca 7740 accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg 7800 gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg 7860 ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg 7920 ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca 7980 ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc 8040 gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga 8100 agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg 8160 accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc 8220 tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg 8280 ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc 8340 aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga 8400 agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc 8460 aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc 8520 ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga 8580 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat 8640 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag 8700 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta 8760 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg 8820 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg 8880 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc 8940 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc 9000 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg 9060 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg 9120 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca 9180 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc 9240 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag 9300 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca 9360 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt 9420 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta 9480 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg 9540 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc 9600 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg 9660 gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgacgg 9720 tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa 9780 gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga 9840 atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg 9900 tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat 9960 gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct 10020 gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt 10080 agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat 10140 ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc 10200 cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg 10260 cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc 10320 catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc 10380 cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat 10440 cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac 10500 ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc 10560 acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag 10620 tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa 10680 tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat 10740 gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat 10800 gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag 10860 cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc 10920 aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg 10980 cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg 11040 tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc 11100 gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa 11160 aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact 11220 cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa 11280 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 11340 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga 11400 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat 11460 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 11520 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 11580 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 11640 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 11700 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 11760 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 11820 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 11880 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 11940 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 12000 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 12060 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 12120 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 12180 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 12240 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 12300 tcaagaagat ccggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac 12360 atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgcc 12414 <210> 29 <211> 12366 <212> DNA <213> Artificial <220> <223> pTVE496 <400> 29 agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60 cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120 acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180 cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240 cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300 ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360 tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420 aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480 agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agctcattct 540 ttgtttccaa gaacttgctt aacaggttcg gtttggtcca catatagtct atgttcttgg 600 atatacctga tgaccgaatt aggtaacaaa tattgtacag acatggcgcg tctgataaat 660 agacgaactt tcgtggaaga aatatcatta tagatgagtt gcttgatgat aagaatattc 720 cttctatgtt catacataat atcatgggat aacaaaaaag accttacatc agaaccagta 780 cgttcgacaa tcaaacaacc gtaattaccg agaatgtgat gtaaatcggc gtccgcccaa 840 acgtttggtt cacccattga ctctattagg tcaccaccag ccagcaacat tattttcaca 900 ccaatttttt ctccagtaac agtagctaca ccacctctct taatattgat ttcgtgattg 960 aaatgatcca agaccttggc agttcttgtg tatgaaggtt gcaatgactc ccatgcatcc 1020 accatcaacc aagatgaggt tctttcgcag gccaattcac acatacgtac tctatggtag 1080 gatggggcca agccttgctt ttgatagtta tcactaacag gggagtaata tccacctatg 1140 acttcaaacc ttgtttgttc agagattgca tctaaagcca tttcaaacat tcttagatgc 1200 aagtaggtga ttggtgaaaa agacccacat gctactatta ctaacggcag tttatttgga 1260 tccagtaatt ttttcgataa tctgtgtgag gggaattcgt agtcttccaa ggttcttgct 1320 tgacgaacga ttccatgtgg aacttcttct aaatcagcaa tctgatattt ctgaacacct 1380 ctagtttctg attctgtggt tgaatctgaa ccgtatctct ccttggatct aaaatccgcg 1440 tcatcatctt cggaagacac ttctgcggaa agtggttgaa agtcgtcttg gttcaatgga 1500 atatgctgat gttttttatc attgccttct ttacgactgt gatgatggtg atgatgatgc 1560 ttaggatgct ttttcttcct cttaatatta aaaggtgcat ctatagagga attcgcatcg 1620 gctaagacgt atggaataat tggaatagat ttgggaattt tagattccgg gtcgggtgga 1680 ggaatcaatt cctcgtctgc agatggcggt ttgaaatccg gagctcttgt gggatccatg 1740 gttttggttt aataagaaga gaaaagagtt cttttgttat ggctgaagta atagagaaat 1800 gagctcgagt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag 1860 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct 1920 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat 1980 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat 2040 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg 2100 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc 2160 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca 2220 ccatgttgac gaagattttc ttcttgtcat tgagtcgtaa aagactctgt atgaactgtt 2280 cgccagtctt cacggcgagt tctgttagat cctcgatctg aatttttgac tccatgtatg 2340 gtgcatatgg cgcgccatat gcccgggccc tgtacagcgg ccgcgttaac gcgtatactc 2400 tagagcgatc gcccgggccg gccatttaaa tgaattcgag ctcggtaccc aaacgcggcc 2460 gcaagctata acttcgtata gcatacatta tacgaagtta ttcgactcta gaggatccca 2520 attcccatgc atggagtcaa agattcaaat agaggacact tctcgaactc ggccgtcgaa 2580 ctcggccgtc gagtacatgg tcgataagaa aaggcaattt gtagatgtta attcccatct 2640 tgaaagaaat atagtttaaa tatttattga taaaataaca agtcaggtat tatagtccaa 2700 gcaaaaacat aaatttattg atgcaagttt aaattcagaa atatttcaat aactgattat 2760 atcagctggt acattgccgt agatgaaaga ctgagtgcga tattatgtgt aatacataaa 2820 ttgatgatat agctagctta gctcatcggg ggatcctaga cgcgtgagat cagatctcgg 2880 tgacgggcag gaccggacgg ggcggtaccg gcaggctgaa gtccagctgc cagaaaccca 2940 cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc 3000 cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc 3060 tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc 3120 ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt 3180 tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga 3240 cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct 3300 gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga 3360 ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggtcca 3420 ttgttcttct ttactctttg tgtgactgag gtttggtcta gtgctttggt catctatata 3480 taatgataac aacaatgaga acaagctttg gagtgatcgg agggtctagg atacatgaga 3540 ttcaagtgga ctaggatcta caccgttgga ttttgagtgt ggatatgtgt gaggttaatt 3600 ttacttggta acggccacaa aggcctaagg agaggtgttg agacccttat cggcttgaac 3660 cgctggaata atgccacgtg gaagataatt ccatgaatct tatcgttatc tatgagtgaa 3720 attgtgtgat ggtggagtgg tgcttgctca ttttacttgc ctggtggact tggccctttc 3780 cttatgggga atttatattt tacttactat agagctttca tacctttttt ttaccttgga 3840 tttagttaat atataatggt atgattcatg aataaaaatg ggaaattttt gaatttgtac 3900 tgctaaatgc ataagattag gtgaaactgt ggaatatata tttttttcat ttaaaagcaa 3960 aatttgcctt ttactagaat tataaatata gaaaaatata taacattcaa ataaaaatga 4020 aaataagaac tttcaaaaaa cagaactatg tttaatgtgt aaagattagt cgcacatcaa 4080 gtcatctgtt acaatatgtt acaacaagtc ataagcccaa caaagttagc acgtctaaat 4140 aaactaaaga gtccacgaaa atattacaaa tcataagccc aacaaagtta ttgatcaaaa 4200 aaaaaaaacg cccaacaaag ctaaacaaag tccaaaaaaa acttctcaag tctccatctt 4260 cctttatgaa cattgaaaac tatacacaaa acaagtcaga taaatctctt tctgggcctg 4320 tcttcccaac ctcctacatc acttccctat cggattgaat gttttacttg taccttttcc 4380 gttgcaatga tattgatagt atgtttgtga aaactaatag ggttaacaat cgaagtcatg 4440 gaatatggat ttggtccaag attttccgag agctttctag tagaaagccc atcaccagaa 4500 atttactagt aaaataaatc accaattagg tttcttatta tgtgccaaat tcaatataat 4560 tatagaggat atttcaaatg aaaacgtatg aatgttatta gtaaatggtc aggtaagaca 4620 ttaaaaaaat cctacgtcag atattcaact ttaaaaattc gatcagtgtg gaattgtaca 4680 aaaatttggg atctactata tatatataat gctttacaac acttggattt ttttttggag 4740 gctggaattt ttaatctaca tatttgtttt ggccatgcac caactcattg tttagtgtaa 4800 tactttgatt ttgtcaaata tatgtgttcg tgtatatttg tataagaatt tctttgacca 4860 tatacacaca cacatatata tatatatata tatattatat atcatgcact tttaattgaa 4920 aaaataatat atatatatat agtgcatttt ttctaacaac catatatgtt gcgattgatc 4980 tgcaaaaata ctgctagagt aatgaaaaat ataatctatt gctgaaatta tctcagatgt 5040 taagattttc ttaaagtaaa ttctttcaaa ttttagctaa aagtcttgta ataactaaag 5100 aataatacac aatctcgacc acggaaaaaa aacacataat aaatttgaat ttcgaccgcg 5160 gtacccggaa ttgggttata attacctcag gtcgaggaat taattcggta cgtacctaat 5220 aacttcgtat agcatacatt atacgaagtt atatggatct cgaggcatta cggcattacg 5280 gcactcgcga gggtcccaat tcgagcatgg agccatttac aattgaatat atcctgccgc 5340 cgctgccgct ttgcacccgg tggagcttgc atgttggttt ctacgcagaa ctgagccggt 5400 taggcagata atttccattg agaactgagc catgtgcacc ttccccccaa cacggtgagc 5460 gacggggcaa cggagtgatc cacatgggac ttttaaacat catccgtcgg atggcgttgc 5520 gagagaagca gtcgatccgt gagatcagcc gacgcaccgg gcaggcgcgc aacacgatcg 5580 caaagtattt gaacgcaggt acaatcgagc cgacgttcac ggtaccggaa cgaccaagca 5640 agctagctta gtaaagccct cgctagattt taatgcggat gttgcgatta cttcgccaac 5700 tattgcgata acaagaaaaa gccagccttt catgatatat ctcccaattt gtgtagggct 5760 tattatgcac gcttaaaaat aataaaagca gacttgacct gatagtttgg ctgtgagcaa 5820 ttatgtgctt agtgcatcta acgcttgagt taagccgcgc cgcgaagcgg cgtcggcttg 5880 aacgaattgt tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga 5940 caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag 6000 cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt 6060 cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca 6120 acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta 6180 aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg 6240 ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat 6300 caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc 6360 caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa 6420 tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa 6480 ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca 6540 aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg 6600 ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg 6660 atacttcggc gatcaccgct tccctcatga tgtttaactt tgttttaggg cgactgccct 6720 gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt 6780 gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga 6840 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg 6900 agcgcatacg ctacttgcat tacagcttac gaaccgaaca ggcttatgtc cactgggttc 6960 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg 7020 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg 7080 cattggcggc cttgctgttc ttctacggca agtgctgtgc acggatctgc cctggcttca 7140 ggagatcgga agacctcggc cgtccgggcg cttgccggtg gtgctgaccc cggatgaagt 7200 ctctagagct ctagagggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg 7260 cccagcttct gtatggaacg ggcatgcgga tcagtgaggg tttgcaactg cgggtcaagg 7320 atctggattt cgatcacggc acgatcatcg tgcgggaggg caagggctcc aaggatcggg 7380 ccttgatgtt acccgagagc ttggcaccca gcctgcgcga gcagggatcg atccaacccc 7440 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca 7500 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc 7560 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg 7620 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc 7680 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg 7740 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac 7800 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc 7860 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc 7920 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg 7980 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg 8040 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc 8100 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc 8160 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca 8220 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg 8280 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt 8340 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc 8400 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct 8460 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg 8520 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt 8580 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc 8640 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc 8700 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg 8760 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag 8820 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct 8880 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac 8940 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga 9000 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag 9060 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc 9120 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc 9180 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt 9240 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg 9300 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg 9360 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag 9420 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca 9480 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa 9540 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc 9600 gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa 9660 tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga 9720 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc 9780 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc 9840 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt 9900 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc 9960 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc 10020 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga 10080 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa 10140 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg 10200 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac 10260 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc 10320 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga 10380 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc 10440 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct 10500 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat 10560 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct 10620 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc 10680 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg 10740 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt 10800 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta 10860 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa 10920 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac 10980 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc 11040 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc 11100 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc 11160 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg 11220 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 11280 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 11340 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc 11400 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 11460 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 11520 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 11580 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 11640 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 11700 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 11760 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 11820 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 11880 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 11940 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 12000 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 12060 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 12120 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 12180 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 12240 aaaaaaagga tctcaagaag atccggaaaa cgcaagcgca aagagaaagc aggtagcttg 12300 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga 12360 attgcc 12366 <210> 30 <211> 12384 <212> DNA <213> Artificial <220> <223> pTVE497 <400> 30 agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60 cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120 acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180 cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240 cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300 ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360 tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420 aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480 agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agctcattct 540 ttgtttccaa gaacttgctt aacaggttcg gtttggtcca catatagtct atgttcttgg 600 atatacctga tgaccgaatt aggtaacaaa tattgtacag acatggcgcg tctgataaat 660 agacgaactt tcgtggaaga aatatcatta tagatgagtt gcttgatgat aagaatattc 720 cttctatgtt catacataat atcatgggat aacaaaaaag accttacatc agaaccagta 780 cgttcgacaa tcaaacaacc gtaattaccg agaatgtgat gtaaatcggc gtccgcccaa 840 acgtttggtt cacccattga ctctattagg tcaccaccag ccagcaacat tattttcaca 900 ccaatttttt ctccagtaac agtagctaca ccacctctct taatattgat ttcgtgattg 960 aaatgatcca agaccttggc agttcttgtg tatgaaggtt gcaatgactc ccatgcatcc 1020 accatcaacc aagatgaggt tctttcgcag gccaattcac acatacgtac tctatggtag 1080 gatggggcca agccttgctt ttgatagtta tcactaacag gggagtaata tccacctatg 1140 acttcaaacc ttgtttgttc agagattgca tctaaagcca tttcaaacat tcttagatgc 1200 aagtaggtga ttggtgaaaa agacccacat gctactatta ctaacggcag tttatttgga 1260 tccagtaatt ttttcgataa tctgtgtgag gggaattcgt agtcttccaa ggttcttgct 1320 tgacgaacga ttccatgtgg aacttcttct aaatcagcaa tctgatattt ctgaacacct 1380 ctagtttctg attctgtggt tgaatctgaa ccgtatctct ccttggatct aaaatccgcg 1440 tcatcatctt cggaagacac ttctgcggaa agtggttgaa agtcgtcttg gttcaatgga 1500 atatgctgat gttttttatc attgccttct ttacgactgt gatgatggtg atgatgatgc 1560 ttaggatgct ttttcttcct cttaatatta aaaggtgcat ctatagagga attcgcatcg 1620 gctaagacgt atggaataat tggaatagat ttgggaattt tagattccgg gtcgggtgga 1680 ggaatcaatt cctcgtctgc agatggcggt ttgaaatccg gagctcttgt aaccttgcgc 1740 ttcttcttgg gatccatggt tttggtttaa taagaagaga aaagagttct tttgttatgg 1800 ctgaagtaat agagaaatga gctcgagtcc tctccaaatg aaatgaactt ccttatatag 1860 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat 1920 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc 1980 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct 2040 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga 2100 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt 2160 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga 2220 cgagagtgtc gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaaa 2280 gactctgtat gaactgttcg ccagtcttca cggcgagttc tgttagatcc tcgatctgaa 2340 tttttgactc catgtatggt gcatatggcg cgccatatgc ccgggccctg tacagcggcc 2400 gcgttaacgc gtatactcta gagcgatcgc ccgggccggc catttaaatg aattcgagct 2460 cggtacccaa acgcggccgc aagctataac ttcgtatagc atacattata cgaagttatt 2520 cgactctaga ggatcccaat tcccatgcat ggagtcaaag attcaaatag aggacacttc 2580 tcgaactcgg ccgtcgaact cggccgtcga gtacatggtc gataagaaaa ggcaatttgt 2640 agatgttaat tcccatcttg aaagaaatat agtttaaata tttattgata aaataacaag 2700 tcaggtatta tagtccaagc aaaaacataa atttattgat gcaagtttaa attcagaaat 2760 atttcaataa ctgattatat cagctggtac attgccgtag atgaaagact gagtgcgata 2820 ttatgtgtaa tacataaatt gatgatatag ctagcttagc tcatcggggg atcctagacg 2880 cgtgagatca gatctcggtg acgggcagga ccggacgggg cggtaccggc aggctgaagt 2940 ccagctgcca gaaacccacg tcatgccagt tcccgtgctt gaagccggcc gcccgcagca 3000 tgccgcgggg ggcatatccg agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc 3060 cgatgacagc gaccacgctc ttgaagccct gtgcctccag ggacttcagc aggtgggtgt 3120 agagcgtgga gcccagtccc gtccgctggt ggcgggggga gacgtacacg gtcgactcgg 3180 ccgtccagtc gtaggcgttg cgtgccttcc aggggcccgc gtaggcgatg ccggcgacct 3240 cgccgtccac ctcggcgacg agccagggat agcgctcccg cagacggacg aggtcgtccg 3300 tccactcctg cggttcctgc ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt 3360 ggttgacgat ggtgcagacc gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg 3420 ggcgtcgttc tgggtccatt gttcttcttt actctttgtg tgactgaggt ttggtctagt 3480 gctttggtca tctatatata atgataacaa caatgagaac aagctttgga gtgatcggag 3540 ggtctaggat acatgagatt caagtggact aggatctaca ccgttggatt ttgagtgtgg 3600 atatgtgtga ggttaatttt acttggtaac ggccacaaag gcctaaggag aggtgttgag 3660 acccttatcg gcttgaaccg ctggaataat gccacgtgga agataattcc atgaatctta 3720 tcgttatcta tgagtgaaat tgtgtgatgg tggagtggtg cttgctcatt ttacttgcct 3780 ggtggacttg gccctttcct tatggggaat ttatatttta cttactatag agctttcata 3840 cctttttttt accttggatt tagttaatat ataatggtat gattcatgaa taaaaatggg 3900 aaatttttga atttgtactg ctaaatgcat aagattaggt gaaactgtgg aatatatatt 3960 tttttcattt aaaagcaaaa tttgcctttt actagaatta taaatataga aaaatatata 4020 acattcaaat aaaaatgaaa ataagaactt tcaaaaaaca gaactatgtt taatgtgtaa 4080 agattagtcg cacatcaagt catctgttac aatatgttac aacaagtcat aagcccaaca 4140 aagttagcac gtctaaataa actaaagagt ccacgaaaat attacaaatc ataagcccaa 4200 caaagttatt gatcaaaaaa aaaaaacgcc caacaaagct aaacaaagtc caaaaaaaac 4260 ttctcaagtc tccatcttcc tttatgaaca ttgaaaacta tacacaaaac aagtcagata 4320 aatctctttc tgggcctgtc ttcccaacct cctacatcac ttccctatcg gattgaatgt 4380 tttacttgta ccttttccgt tgcaatgata ttgatagtat gtttgtgaaa actaataggg 4440 ttaacaatcg aagtcatgga atatggattt ggtccaagat tttccgagag ctttctagta 4500 gaaagcccat caccagaaat ttactagtaa aataaatcac caattaggtt tcttattatg 4560 tgccaaattc aatataatta tagaggatat ttcaaatgaa aacgtatgaa tgttattagt 4620 aaatggtcag gtaagacatt aaaaaaatcc tacgtcagat attcaacttt aaaaattcga 4680 tcagtgtgga attgtacaaa aatttgggat ctactatata tatataatgc tttacaacac 4740 ttggattttt ttttggaggc tggaattttt aatctacata tttgttttgg ccatgcacca 4800 actcattgtt tagtgtaata ctttgatttt gtcaaatata tgtgttcgtg tatatttgta 4860 taagaatttc tttgaccata tacacacaca catatatata tatatatata tattatatat 4920 catgcacttt taattgaaaa aataatatat atatatatag tgcatttttt ctaacaacca 4980 tatatgttgc gattgatctg caaaaatact gctagagtaa tgaaaaatat aatctattgc 5040 tgaaattatc tcagatgtta agattttctt aaagtaaatt ctttcaaatt ttagctaaaa 5100 gtcttgtaat aactaaagaa taatacacaa tctcgaccac ggaaaaaaaa cacataataa 5160 atttgaattt cgaccgcggt acccggaatt gggttataat tacctcaggt cgaggaatta 5220 attcggtacg tacctaataa cttcgtatag catacattat acgaagttat atggatctcg 5280 aggcattacg gcattacggc actcgcgagg gtcccaattc gagcatggag ccatttacaa 5340 ttgaatatat cctgccgccg ctgccgcttt gcacccggtg gagcttgcat gttggtttct 5400 acgcagaact gagccggtta ggcagataat ttccattgag aactgagcca tgtgcacctt 5460 ccccccaaca cggtgagcga cggggcaacg gagtgatcca catgggactt ttaaacatca 5520 tccgtcggat ggcgttgcga gagaagcagt cgatccgtga gatcagccga cgcaccgggc 5580 aggcgcgcaa cacgatcgca aagtatttga acgcaggtac aatcgagccg acgttcacgg 5640 taccggaacg accaagcaag ctagcttagt aaagccctcg ctagatttta atgcggatgt 5700 tgcgattact tcgccaacta ttgcgataac aagaaaaagc cagcctttca tgatatatct 5760 cccaatttgt gtagggctta ttatgcacgc ttaaaaataa taaaagcaga cttgacctga 5820 tagtttggct gtgagcaatt atgtgcttag tgcatctaac gcttgagtta agccgcgccg 5880 cgaagcggcg tcggcttgaa cgaattgtta gacattattt gccgactacc ttggtgatct 5940 cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt 6000 cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca 6060 ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc 6120 tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg 6180 gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg 6240 gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg 6300 tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt 6360 cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga 6420 tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg 6480 aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta 6540 cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg 6600 agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta 6660 cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatgatg tttaactttg 6720 ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac 6780 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac 6840 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg 6900 ttctggacca gttgcgtgag cgcatacgct acttgcatta cagcttacga accgaacagg 6960 cttatgtcca ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct 7020 tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc aaggtttcgg 7080 tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag tgctgtgcac 7140 ggatctgccc tggcttcagg agatcggaag acctcggccg tccgggcgct tgccggtggt 7200 gctgaccccg gatgaagtct ctagagctct agagggttcg catcctcggt tttctggaag 7260 gcgagcatcg tttgttcgcc cagcttctgt atggaacggg catgcggatc agtgagggtt 7320 tgcaactgcg ggtcaaggat ctggatttcg atcacggcac gatcatcgtg cgggagggca 7380 agggctccaa ggatcgggcc ttgatgttac ccgagagctt ggcacccagc ctgcgcgagc 7440 agggatcgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc 7500 cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc 7560 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact 7620 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc 7680 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct 7740 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca 7800 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg 7860 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc 7920 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga 7980 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg 8040 ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg 8100 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg 8160 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag 8220 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg 8280 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga 8340 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg 8400 gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg 8460 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg 8520 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc 8580 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct 8640 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc 8700 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc 8760 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg 8820 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc 8880 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg 8940 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc 9000 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc 9060 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag 9120 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt 9180 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa 9240 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa 9300 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca 9360 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga 9420 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc 9480 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc 9540 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga 9600 acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga 9660 acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg 9720 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca 9780 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg 9840 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg 9900 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg 9960 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt 10020 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc 10080 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa 10140 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga 10200 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac 10260 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg 10320 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag 10380 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat 10440 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc 10500 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag 10560 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg 10620 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga 10680 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc 10740 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa 10800 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat 10860 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat 10920 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact 10980 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga 11040 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg 11100 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc 11160 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct 11220 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 11280 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 11340 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 11400 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 11460 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 11520 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 11580 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 11640 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 11700 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 11760 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 11820 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 11880 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 11940 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 12000 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 12060 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 12120 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 12180 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 12240 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat ccggaaaacg caagcgcaaa 12300 gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg 12360 gacagcaagc gaaccggaat tgcc 12384 <210> 31 <211> 12348 <212> DNA <213> Artificial <220> <223> pTVE 500 <400> 31 agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60 cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120 acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180 cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240 cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300 ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360 tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420 aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480 agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agctcactct 540 ttgctatcca agacctgctt gaccggttca ctttgattaa tgtatagatt atactcttgg 600 atgtaacgga tgacagagtt tggaagaaga tattgaactg acattccacg tctgatgaaa 660 agccgcactt tcgtagagga aatatcattg taaataagtt gtttgataat aaggatattt 720 cttctgtgtt catacatgat atcatgggaa agcgagaagg acctaacatc agaaccagtc 780 ctttccacga tcaaacatcc ataattaccc aaaatatggt gcaggtctga atcagcccac 840 acatgaggct cgcccatgga ttcgataaga tcaccgcctg ccaataacat gatttttacg 900 cccatttttt caccatctac agtcatgatt ccacctctct tgatatttat ttcatgattg 960 aaatggtcca agacttttgc tgtccttgta taacttgatt gtaaagattc ccaggcatca 1020 accattaacc aagatgatgt ccgctcgcat gctaattcgc acatgcggac acgatgataa 1080 gctggggcta accctcgctt ttgatagtta tcacttactg gagaaaaata accaccaacc 1140 acttcaaaac gcgtttgctc attgatatca tctaaagcca tttcaaacat tctcaaatgt 1200 aggtatgtta tgggagaaaa tgatccacaa gcaacgatga tcagaggcag tttttcagga 1260 tcttgtaact ttttcgtcaa tctgtgtaca ggaaattcgt aatcttcaat agttctggct 1320 tgtcttacaa ttgtgtgagg aacttcttcc aaatcagcaa tttggctctt cagtaccccc 1380 aaattcccag tcatcgtaac atcctgtaga gtagcgtcaa ttccattcga ttgcccttca 1440 ctttcctcct ccgatgatac atcccgagat agtggctgga agtcactagt atttaatgga 1500 atatggtcca ttcggttgct tgagttggcg ttttttttcc ttgacgacag ggttttgtaa 1560 atgtcgagat tgaaaggagc atcgatcgaa gaattataat cagctaaaac atatggaact 1620 atgggtccag attttggcat cgtatgtgtt ggatctggcg gtggttgtag ttcttcattt 1680 ggctgtggcg gtttaaaatc gggtgctttg gtgggatcca tggttttggt ttaataagaa 1740 gagaaaagag ttcttttgtt atggctgaag taatagagaa atgagctcga gtcctctcca 1800 aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt 1860 catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa 1920 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg 1980 cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac 2040 cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga 2100 ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg 2160 tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttg acgaagattt 2220 tcttcttgtc attgagtcgt aaaagactct gtatgaactg ttcgccagtc ttcacggcga 2280 gttctgttag atcctcgatc tgaatttttg actccatgta tggtgcatat ggcgcgccat 2340 atgcccgggc cctgtacagc ggccgcgtta acgcgtatac tctagagcga tcgcccgggc 2400 cggccattta aatgaattcg agctcggtac ccaaacgcgg ccgcaagcta taacttcgta 2460 tagcatacat tatacgaagt tattcgactc tagaggatcc caattcccat gcatggagtc 2520 aaagattcaa atagaggaca cttctcgaac tcggccgtcg aactcggccg tcgagtacat 2580 ggtcgataag aaaaggcaat ttgtagatgt taattcccat cttgaaagaa atatagttta 2640 aatatttatt gataaaataa caagtcaggt attatagtcc aagcaaaaac ataaatttat 2700 tgatgcaagt ttaaattcag aaatatttca ataactgatt atatcagctg gtacattgcc 2760 gtagatgaaa gactgagtgc gatattatgt gtaatacata aattgatgat atagctagct 2820 tagctcatcg ggggatccta gacgcgtgag atcagatctc ggtgacgggc aggaccggac 2880 ggggcggtac cggcaggctg aagtccagct gccagaaacc cacgtcatgc cagttcccgt 2940 gcttgaagcc ggccgcccgc agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc 3000 gcacgctcgg gtcgttgggc agcccgatga cagcgaccac gctcttgaag ccctgtgcct 3060 ccagggactt cagcaggtgg gtgtagagcg tggagcccag tcccgtccgc tggtggcggg 3120 gggagacgta cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc ttccaggggc 3180 ccgcgtaggc gatgccggcg acctcgccgt ccacctcggc gacgagccag ggatagcgct 3240 cccgcagacg gacgaggtcg tccgtccact cctgcggttc ctgcggctcg gtacggaagt 3300 tgaccgtgct tgtctcgatg tagtggttga cgatggtgca gaccgccggc atgtccgcct 3360 cggtggcacg gcggatgtcg gccgggcgtc gttctgggtc cattgttctt ctttactctt 3420 tgtgtgactg aggtttggtc tagtgctttg gtcatctata tataatgata acaacaatga 3480 gaacaagctt tggagtgatc ggagggtcta ggatacatga gattcaagtg gactaggatc 3540 tacaccgttg gattttgagt gtggatatgt gtgaggttaa ttttacttgg taacggccac 3600 aaaggcctaa ggagaggtgt tgagaccctt atcggcttga accgctggaa taatgccacg 3660 tggaagataa ttccatgaat cttatcgtta tctatgagtg aaattgtgtg atggtggagt 3720 ggtgcttgct cattttactt gcctggtgga cttggccctt tccttatggg gaatttatat 3780 tttacttact atagagcttt catacctttt ttttaccttg gatttagtta atatataatg 3840 gtatgattca tgaataaaaa tgggaaattt ttgaatttgt actgctaaat gcataagatt 3900 aggtgaaact gtggaatata tatttttttc atttaaaagc aaaatttgcc ttttactaga 3960 attataaata tagaaaaata tataacattc aaataaaaat gaaaataaga actttcaaaa 4020 aacagaacta tgtttaatgt gtaaagatta gtcgcacatc aagtcatctg ttacaatatg 4080 ttacaacaag tcataagccc aacaaagtta gcacgtctaa ataaactaaa gagtccacga 4140 aaatattaca aatcataagc ccaacaaagt tattgatcaa aaaaaaaaaa cgcccaacaa 4200 agctaaacaa agtccaaaaa aaacttctca agtctccatc ttcctttatg aacattgaaa 4260 actatacaca aaacaagtca gataaatctc tttctgggcc tgtcttccca acctcctaca 4320 tcacttccct atcggattga atgttttact tgtacctttt ccgttgcaat gatattgata 4380 gtatgtttgt gaaaactaat agggttaaca atcgaagtca tggaatatgg atttggtcca 4440 agattttccg agagctttct agtagaaagc ccatcaccag aaatttacta gtaaaataaa 4500 tcaccaatta ggtttcttat tatgtgccaa attcaatata attatagagg atatttcaaa 4560 tgaaaacgta tgaatgttat tagtaaatgg tcaggtaaga cattaaaaaa atcctacgtc 4620 agatattcaa ctttaaaaat tcgatcagtg tggaattgta caaaaatttg ggatctacta 4680 tatatatata atgctttaca acacttggat ttttttttgg aggctggaat ttttaatcta 4740 catatttgtt ttggccatgc accaactcat tgtttagtgt aatactttga ttttgtcaaa 4800 tatatgtgtt cgtgtatatt tgtataagaa tttctttgac catatacaca cacacatata 4860 tatatatata tatatattat atatcatgca cttttaattg aaaaaataat atatatatat 4920 atagtgcatt ttttctaaca accatatatg ttgcgattga tctgcaaaaa tactgctaga 4980 gtaatgaaaa atataatcta ttgctgaaat tatctcagat gttaagattt tcttaaagta 5040 aattctttca aattttagct aaaagtcttg taataactaa agaataatac acaatctcga 5100 ccacggaaaa aaaacacata ataaatttga atttcgaccg cggtacccgg aattgggtta 5160 taattacctc aggtcgagga attaattcgg tacgtaccta ataacttcgt atagcataca 5220 ttatacgaag ttatatggat ctcgaggcat tacggcatta cggcactcgc gagggtccca 5280 attcgagcat ggagccattt acaattgaat atatcctgcc gccgctgccg ctttgcaccc 5340 ggtggagctt gcatgttggt ttctacgcag aactgagccg gttaggcaga taatttccat 5400 tgagaactga gccatgtgca ccttcccccc aacacggtga gcgacggggc aacggagtga 5460 tccacatggg acttttaaac atcatccgtc ggatggcgtt gcgagagaag cagtcgatcc 5520 gtgagatcag ccgacgcacc gggcaggcgc gcaacacgat cgcaaagtat ttgaacgcag 5580 gtacaatcga gccgacgttc acggtaccgg aacgaccaag caagctagct tagtaaagcc 5640 ctcgctagat tttaatgcgg atgttgcgat tacttcgcca actattgcga taacaagaaa 5700 aagccagcct ttcatgatat atctcccaat ttgtgtaggg cttattatgc acgcttaaaa 5760 ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc ttagtgcatc 5820 taacgcttga gttaagccgc gccgcgaagc ggcgtcggct tgaacgaatt gttagacatt 5880 atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat 5940 ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta 6000 tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg 6060 gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc 6120 gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct 6180 caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg 6240 caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg 6300 gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct 6360 tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc 6420 ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc 6480 gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg 6540 gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga 6600 gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg 6660 cttccctcat gatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc 6720 tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag 6780 gcatagactg taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt 6840 taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc 6900 attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgccttc atccgtttcc 6960 acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc 7020 tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg gccttgctgt 7080 tcttctacgg caagtgctgt gcacggatct gccctggctt caggagatcg gaagacctcg 7140 gccgtccggg cgcttgccgg tggtgctgac cccggatgaa gtctctagag ctctagaggg 7200 ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt cgcccagctt ctgtatggaa 7260 cgggcatgcg gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat ttcgatcacg 7320 gcacgatcat cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg ttacccgaga 7380 gcttggcacc cagcctgcgc gagcagggat cgatccaacc cctccgctgc tatagtgcag 7440 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag 7500 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc 7560 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg 7620 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac 7680 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca 7740 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga 7800 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc 7860 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca 7920 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc 7980 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg 8040 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg 8100 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc 8160 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc 8220 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg 8280 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat 8340 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt 8400 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag 8460 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa 8520 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa 8580 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc 8640 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat 8700 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc 8760 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac 8820 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca 8880 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac 8940 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt 9000 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc 9060 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact 9120 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc 9180 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa 9240 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg 9300 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg 9360 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc 9420 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga 9480 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc 9540 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc 9600 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg acggtcgcaa 9660 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa 9720 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg 9780 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc 9840 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta 9900 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa 9960 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt 10020 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt 10080 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg 10140 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa 10200 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca 10260 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat 10320 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct 10380 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca 10440 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg 10500 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag 10560 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct 10620 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta 10680 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg 10740 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta 10800 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc 10860 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt 10920 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata 10980 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg 11040 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc 11100 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg 11160 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct 11220 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 11280 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt 11340 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact 11400 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 11460 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 11520 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 11580 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 11640 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 11700 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 11760 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 11820 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 11880 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 11940 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 12000 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 12060 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 12120 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 12180 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 12240 agatccggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg 12300 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgcc 12348 <210> 32 <211> 12366 <212> DNA <213> Artificial <220> <223> pTVE 501 <400> 32 agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60 cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120 acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180 cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240 cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300 ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360 tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420 aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480 agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agctcactct 540 ttgctatcca agacctgctt gaccggttca ctttgattaa tgtatagatt atactcttgg 600 atgtaacgga tgacagagtt tggaagaaga tattgaactg acattccacg tctgatgaaa 660 agccgcactt tcgtagagga aatatcattg taaataagtt gtttgataat aaggatattt 720 cttctgtgtt catacatgat atcatgggaa agcgagaagg acctaacatc agaaccagtc 780 ctttccacga tcaaacatcc ataattaccc aaaatatggt gcaggtctga atcagcccac 840 acatgaggct cgcccatgga ttcgataaga tcaccgcctg ccaataacat gatttttacg 900 cccatttttt caccatctac agtcatgatt ccacctctct tgatatttat ttcatgattg 960 aaatggtcca agacttttgc tgtccttgta taacttgatt gtaaagattc ccaggcatca 1020 accattaacc aagatgatgt ccgctcgcat gctaattcgc acatgcggac acgatgataa 1080 gctggggcta accctcgctt ttgatagtta tcacttactg gagaaaaata accaccaacc 1140 acttcaaaac gcgtttgctc attgatatca tctaaagcca tttcaaacat tctcaaatgt 1200 aggtatgtta tgggagaaaa tgatccacaa gcaacgatga tcagaggcag tttttcagga 1260 tcttgtaact ttttcgtcaa tctgtgtaca ggaaattcgt aatcttcaat agttctggct 1320 tgtcttacaa ttgtgtgagg aacttcttcc aaatcagcaa tttggctctt cagtaccccc 1380 aaattcccag tcatcgtaac atcctgtaga gtagcgtcaa ttccattcga ttgcccttca 1440 ctttcctcct ccgatgatac atcccgagat agtggctgga agtcactagt atttaatgga 1500 atatggtcca ttcggttgct tgagttggcg ttttttttcc ttgacgacag ggttttgtaa 1560 atgtcgagat tgaaaggagc atcgatcgaa gaattataat cagctaaaac atatggaact 1620 atgggtccag attttggcat cgtatgtgtt ggatctggcg gtggttgtag ttcttcattt 1680 ggctgtggcg gtttaaaatc gggtgctttg gtaaccttgc gcttcttctt gggatccatg 1740 gttttggttt aataagaaga gaaaagagtt cttttgttat ggctgaagta atagagaaat 1800 gagctcgagt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag 1860 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct 1920 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat 1980 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat 2040 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg 2100 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc 2160 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca 2220 ccatgttgac gaagattttc ttcttgtcat tgagtcgtaa aagactctgt atgaactgtt 2280 cgccagtctt cacggcgagt tctgttagat cctcgatctg aatttttgac tccatgtatg 2340 gtgcatatgg cgcgccatat gcccgggccc tgtacagcgg ccgcgttaac gcgtatactc 2400 tagagcgatc gcccgggccg gccatttaaa tgaattcgag ctcggtaccc aaacgcggcc 2460 gcaagctata acttcgtata gcatacatta tacgaagtta ttcgactcta gaggatccca 2520 attcccatgc atggagtcaa agattcaaat agaggacact tctcgaactc ggccgtcgaa 2580 ctcggccgtc gagtacatgg tcgataagaa aaggcaattt gtagatgtta attcccatct 2640 tgaaagaaat atagtttaaa tatttattga taaaataaca agtcaggtat tatagtccaa 2700 gcaaaaacat aaatttattg atgcaagttt aaattcagaa atatttcaat aactgattat 2760 atcagctggt acattgccgt agatgaaaga ctgagtgcga tattatgtgt aatacataaa 2820 ttgatgatat agctagctta gctcatcggg ggatcctaga cgcgtgagat cagatctcgg 2880 tgacgggcag gaccggacgg ggcggtaccg gcaggctgaa gtccagctgc cagaaaccca 2940 cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc 3000 cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc 3060 tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc 3120 ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt 3180 tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga 3240 cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct 3300 gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga 3360 ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggtcca 3420 ttgttcttct ttactctttg tgtgactgag gtttggtcta gtgctttggt catctatata 3480 taatgataac aacaatgaga acaagctttg gagtgatcgg agggtctagg atacatgaga 3540 ttcaagtgga ctaggatcta caccgttgga ttttgagtgt ggatatgtgt gaggttaatt 3600 ttacttggta acggccacaa aggcctaagg agaggtgttg agacccttat cggcttgaac 3660 cgctggaata atgccacgtg gaagataatt ccatgaatct tatcgttatc tatgagtgaa 3720 attgtgtgat ggtggagtgg tgcttgctca ttttacttgc ctggtggact tggccctttc 3780 cttatgggga atttatattt tacttactat agagctttca tacctttttt ttaccttgga 3840 tttagttaat atataatggt atgattcatg aataaaaatg ggaaattttt gaatttgtac 3900 tgctaaatgc ataagattag gtgaaactgt ggaatatata tttttttcat ttaaaagcaa 3960 aatttgcctt ttactagaat tataaatata gaaaaatata taacattcaa ataaaaatga 4020 aaataagaac tttcaaaaaa cagaactatg tttaatgtgt aaagattagt cgcacatcaa 4080 gtcatctgtt acaatatgtt acaacaagtc ataagcccaa caaagttagc acgtctaaat 4140 aaactaaaga gtccacgaaa atattacaaa tcataagccc aacaaagtta ttgatcaaaa 4200 aaaaaaaacg cccaacaaag ctaaacaaag tccaaaaaaa acttctcaag tctccatctt 4260 cctttatgaa cattgaaaac tatacacaaa acaagtcaga taaatctctt tctgggcctg 4320 tcttcccaac ctcctacatc acttccctat cggattgaat gttttacttg taccttttcc 4380 gttgcaatga tattgatagt atgtttgtga aaactaatag ggttaacaat cgaagtcatg 4440 gaatatggat ttggtccaag attttccgag agctttctag tagaaagccc atcaccagaa 4500 atttactagt aaaataaatc accaattagg tttcttatta tgtgccaaat tcaatataat 4560 tatagaggat atttcaaatg aaaacgtatg aatgttatta gtaaatggtc aggtaagaca 4620 ttaaaaaaat cctacgtcag atattcaact ttaaaaattc gatcagtgtg gaattgtaca 4680 aaaatttggg atctactata tatatataat gctttacaac acttggattt ttttttggag 4740 gctggaattt ttaatctaca tatttgtttt ggccatgcac caactcattg tttagtgtaa 4800 tactttgatt ttgtcaaata tatgtgttcg tgtatatttg tataagaatt tctttgacca 4860 tatacacaca cacatatata tatatatata tatattatat atcatgcact tttaattgaa 4920 aaaataatat atatatatat agtgcatttt ttctaacaac catatatgtt gcgattgatc 4980 tgcaaaaata ctgctagagt aatgaaaaat ataatctatt gctgaaatta tctcagatgt 5040 taagattttc ttaaagtaaa ttctttcaaa ttttagctaa aagtcttgta ataactaaag 5100 aataatacac aatctcgacc acggaaaaaa aacacataat aaatttgaat ttcgaccgcg 5160 gtacccggaa ttgggttata attacctcag gtcgaggaat taattcggta cgtacctaat 5220 aacttcgtat agcatacatt atacgaagtt atatggatct cgaggcatta cggcattacg 5280 gcactcgcga gggtcccaat tcgagcatgg agccatttac aattgaatat atcctgccgc 5340 cgctgccgct ttgcacccgg tggagcttgc atgttggttt ctacgcagaa ctgagccggt 5400 taggcagata atttccattg agaactgagc catgtgcacc ttccccccaa cacggtgagc 5460 gacggggcaa cggagtgatc cacatgggac ttttaaacat catccgtcgg atggcgttgc 5520 gagagaagca gtcgatccgt gagatcagcc gacgcaccgg gcaggcgcgc aacacgatcg 5580 caaagtattt gaacgcaggt acaatcgagc cgacgttcac ggtaccggaa cgaccaagca 5640 agctagctta gtaaagccct cgctagattt taatgcggat gttgcgatta cttcgccaac 5700 tattgcgata acaagaaaaa gccagccttt catgatatat ctcccaattt gtgtagggct 5760 tattatgcac gcttaaaaat aataaaagca gacttgacct gatagtttgg ctgtgagcaa 5820 ttatgtgctt agtgcatcta acgcttgagt taagccgcgc cgcgaagcgg cgtcggcttg 5880 aacgaattgt tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga 5940 caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag 6000 cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt 6060 cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca 6120 acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta 6180 aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg 6240 ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat 6300 caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc 6360 caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa 6420 tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa 6480 ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca 6540 aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg 6600 ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg 6660 atacttcggc gatcaccgct tccctcatga tgtttaactt tgttttaggg cgactgccct 6720 gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt 6780 gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga 6840 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg 6900 agcgcatacg ctacttgcat tacagcttac gaaccgaaca ggcttatgtc cactgggttc 6960 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg 7020 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg 7080 cattggcggc cttgctgttc ttctacggca agtgctgtgc acggatctgc cctggcttca 7140 ggagatcgga agacctcggc cgtccgggcg cttgccggtg gtgctgaccc cggatgaagt 7200 ctctagagct ctagagggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg 7260 cccagcttct gtatggaacg ggcatgcgga tcagtgaggg tttgcaactg cgggtcaagg 7320 atctggattt cgatcacggc acgatcatcg tgcgggaggg caagggctcc aaggatcggg 7380 ccttgatgtt acccgagagc ttggcaccca gcctgcgcga gcagggatcg atccaacccc 7440 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca 7500 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc 7560 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg 7620 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc 7680 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg 7740 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac 7800 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc 7860 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc 7920 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg 7980 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg 8040 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc 8100 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc 8160 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca 8220 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg 8280 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt 8340 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc 8400 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct 8460 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg 8520 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt 8580 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc 8640 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc 8700 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg 8760 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag 8820 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct 8880 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac 8940 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga 9000 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag 9060 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc 9120 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc 9180 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt 9240 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg 9300 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg 9360 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag 9420 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca 9480 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa 9540 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc 9600 gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa 9660 tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga 9720 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc 9780 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc 9840 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt 9900 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc 9960 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc 10020 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga 10080 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa 10140 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg 10200 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac 10260 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc 10320 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga 10380 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc 10440 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct 10500 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat 10560 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct 10620 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc 10680 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg 10740 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt 10800 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta 10860 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa 10920 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac 10980 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc 11040 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc 11100 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc 11160 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg 11220 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 11280 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 11340 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc 11400 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 11460 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 11520 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 11580 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 11640 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 11700 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 11760 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 11820 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 11880 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 11940 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 12000 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 12060 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 12120 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 12180 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 12240 aaaaaaagga tctcaagaag atccggaaaa cgcaagcgca aagagaaagc aggtagcttg 12300 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga 12360 attgcc 12366 <210> 33 <211> 13305 <212> DNA <213> Artificial <220> <223> pTVE502 <400> 33 agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60 cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120 acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180 cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240 cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300 ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360 tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420 aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480 agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agcctaatca 540 atagacataa tgtcaagcgt tgagccttta tgtgcttcac actgctcgac aacttcatca 600 atttttcttg aagcccatgg aaatcttggg ttgattaaga aaggacgtaa gtcaaatctg 660 ttgtcttctg gtgaatactg ttcagcatga taactaggag ttaaaacagt ttgcttgtgt 720 ctgttgatgg catagaagaa gaaaaatctt ttcacctttt cagatatttg acgaggtgtt 780 aactttgggg accattgatg aagaagtttt aagaacatag aataaggacc acatttttca 840 acctttctta ggtaaccaaa cacgcccaat tcttcatacg tcatccccat atctatctca 900 tccgattgaa cgtaatcttt agtcataggt tctaattctg cagttggtgt agcgtttaaa 960 aagtcattca agattggcat gttatattgt tttgatgcgt aggcaatgaa tcttttcaag 1020 tcagtttttg aaataccccc aatagggttg atatctgcgg aggagcagtc atattttgtt 1080 agatacccac gtaagcactc atcaacattt gcgctaccaa gtactaacaa tccacccgag 1140 tttgggatac cacgaaccca cggcaacagt tgcgcaaaaa gataagaaag aaccattctt 1200 agacgcgcct ggatgttttg taaagccaag ttctcgattt gagatccccc aaatattttg 1260 tatattggtt ttttgccagt ggctacttcg aataaggaca ccacactgga taccaatgag 1320 tccatcttta aatccacgtg gtaagatcca attgcattgg aaaggtcctt tgctctgttt 1380 cttgtctcct tggatgaatt ttccgtaccc atgaaacagg agtgaaatat ttttgaggct 1440 agatcctgtg gactgtctgg aatccaatca tcgccgctac gtgttatctt acgaacgtct 1500 ttgataactt gctcatttcc attttgagca gcgtcggtca ctaaacggca catagagtgg 1560 acaatcattg cagttgcaca agagtcaatg cccccagata agggaaggaa aaaccctgtt 1620 ccgttacaac gtcttaaata atcccacatc cagcaagcag gtcccagtgc aatttcttcc 1680 tcaggagagt gatagaaagg ctcgcggact tttgttggac acactgtagg atcaaatctg 1740 gaggtcatta aagccaattc tacaggaata tcaatacgct tgaactttat ttctgccaag 1800 gaggcttgta ggccacgaga catgacagct gcacgataac tcctcacctc ttctaggtcc 1860 acagtagcag taactacttc cacatcatct agcgaaaatt gtgaaccttg ggctacaatt 1920 gtaccattga tggcaattag tgcacagcca tcataatata atctgtcacc atcacaacct 1980 ctttgatttg catacaagta aacaccacca caacgtttag tggcatttaa aattaggtct 2040 aaccttttat ttaacttacg cagttcatga tgagaaccag atgagtttgt catgatttcc 2100 acaccatcta aagacatggc gatgtggggg gattgaggtg taaacaattc ttcacaagtt 2160 tctgtaccaa tgcatgtatc caatgaattt atcacagcgt ccccaaatgg cacaagtctc 2220 tggccggtaa ctttctgaat ctcaggtgga aggataaagt cctccaccac gccaggtttc 2280 atccaaggtg tgaaaaatct catttcccta tagttaccat cattagctaa ccaaatctta 2340 ggtcttatga acaatatctc accatccaag gataacaaac gacaattata acgaacattc 2400 ttgtgtagaa cgggcatacc aatgtcaagt attaatccat gggtttcttt attcttaatg 2460 atttgagcat acatttccca tgaatgaagg caaacgtcat tttctaaaaa atgatctaaa 2520 catccgtagc cagttatttc cagttctggg ccgacacgta acctggcacc cctctctttg 2580 gcaatcttaa tggactgtag gatacggtct ctattacctt caaaatctag ggcccattga 2640 ttcaagttgc atgtagctaa agtgataaga tgtgccatgg ttttggttta ataagaagag 2700 aaaagagttc ttttgttatg gctgaagtaa tagagaaatg agctcgagtc ctctccaaat 2760 gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat 2820 cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt 2880 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag 2940 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt 3000 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt 3060 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat 3120 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttgacg aagattttct 3180 tcttgtcatt gagtcgtaaa agactctgta tgaactgttc gccagtcttc acggcgagtt 3240 ctgttagatc ctcgatctga atttttgact ccatgtatgg tgcatatggc gcgccatatg 3300 cccgggccct gtacagcggc cgcgttaacg cgtatactct agagcgatcg cccgggccgg 3360 ccatttaaat gaattcgagc tcggtaccca aacgcggccg caagctataa cttcgtatag 3420 catacattat acgaagttat tcgactctag aggatcccaa ttcccatgca tggagtcaaa 3480 gattcaaata gaggacactt ctcgaactcg gccgtcgaac tcggccgtcg agtacatggt 3540 cgataagaaa aggcaatttg tagatgttaa ttcccatctt gaaagaaata tagtttaaat 3600 atttattgat aaaataacaa gtcaggtatt atagtccaag caaaaacata aatttattga 3660 tgcaagttta aattcagaaa tatttcaata actgattata tcagctggta cattgccgta 3720 gatgaaagac tgagtgcgat attatgtgta atacataaat tgatgatata gctagcttag 3780 ctcatcgggg gatcctagac gcgtgagatc agatctcggt gacgggcagg accggacggg 3840 gcggtaccgg caggctgaag tccagctgcc agaaacccac gtcatgccag ttcccgtgct 3900 tgaagccggc cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg tgcatgcgca 3960 cgctcgggtc gttgggcagc ccgatgacag cgaccacgct cttgaagccc tgtgcctcca 4020 gggacttcag caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg tggcgggggg 4080 agacgtacac ggtcgactcg gccgtccagt cgtaggcgtt gcgtgccttc caggggcccg 4140 cgtaggcgat gccggcgacc tcgccgtcca cctcggcgac gagccaggga tagcgctccc 4200 gcagacggac gaggtcgtcc gtccactcct gcggttcctg cggctcggta cggaagttga 4260 ccgtgcttgt ctcgatgtag tggttgacga tggtgcagac cgccggcatg tccgcctcgg 4320 tggcacggcg gatgtcggcc gggcgtcgtt ctgggtccat tgttcttctt tactctttgt 4380 gtgactgagg tttggtctag tgctttggtc atctatatat aatgataaca acaatgagaa 4440 caagctttgg agtgatcgga gggtctagga tacatgagat tcaagtggac taggatctac 4500 accgttggat tttgagtgtg gatatgtgtg aggttaattt tacttggtaa cggccacaaa 4560 ggcctaagga gaggtgttga gacccttatc ggcttgaacc gctggaataa tgccacgtgg 4620 aagataattc catgaatctt atcgttatct atgagtgaaa ttgtgtgatg gtggagtggt 4680 gcttgctcat tttacttgcc tggtggactt ggccctttcc ttatggggaa tttatatttt 4740 acttactata gagctttcat accttttttt taccttggat ttagttaata tataatggta 4800 tgattcatga ataaaaatgg gaaatttttg aatttgtact gctaaatgca taagattagg 4860 tgaaactgtg gaatatatat ttttttcatt taaaagcaaa atttgccttt tactagaatt 4920 ataaatatag aaaaatatat aacattcaaa taaaaatgaa aataagaact ttcaaaaaac 4980 agaactatgt ttaatgtgta aagattagtc gcacatcaag tcatctgtta caatatgtta 5040 caacaagtca taagcccaac aaagttagca cgtctaaata aactaaagag tccacgaaaa 5100 tattacaaat cataagccca acaaagttat tgatcaaaaa aaaaaaacgc ccaacaaagc 5160 taaacaaagt ccaaaaaaaa cttctcaagt ctccatcttc ctttatgaac attgaaaact 5220 atacacaaaa caagtcagat aaatctcttt ctgggcctgt cttcccaacc tcctacatca 5280 cttccctatc ggattgaatg ttttacttgt accttttccg ttgcaatgat attgatagta 5340 tgtttgtgaa aactaatagg gttaacaatc gaagtcatgg aatatggatt tggtccaaga 5400 ttttccgaga gctttctagt agaaagccca tcaccagaaa tttactagta aaataaatca 5460 ccaattaggt ttcttattat gtgccaaatt caatataatt atagaggata tttcaaatga 5520 aaacgtatga atgttattag taaatggtca ggtaagacat taaaaaaatc ctacgtcaga 5580 tattcaactt taaaaattcg atcagtgtgg aattgtacaa aaatttggga tctactatat 5640 atatataatg ctttacaaca cttggatttt tttttggagg ctggaatttt taatctacat 5700 atttgttttg gccatgcacc aactcattgt ttagtgtaat actttgattt tgtcaaatat 5760 atgtgttcgt gtatatttgt ataagaattt ctttgaccat atacacacac acatatatat 5820 atatatatat atattatata tcatgcactt ttaattgaaa aaataatata tatatatata 5880 gtgcattttt tctaacaacc atatatgttg cgattgatct gcaaaaatac tgctagagta 5940 atgaaaaata taatctattg ctgaaattat ctcagatgtt aagattttct taaagtaaat 6000 tctttcaaat tttagctaaa agtcttgtaa taactaaaga ataatacaca atctcgacca 6060 cggaaaaaaa acacataata aatttgaatt tcgaccgcgg tacccggaat tgggttataa 6120 ttacctcagg tcgaggaatt aattcggtac gtacctaata acttcgtata gcatacatta 6180 tacgaagtta tatggatctc gaggcattac ggcattacgg cactcgcgag ggtcccaatt 6240 cgagcatgga gccatttaca attgaatata tcctgccgcc gctgccgctt tgcacccggt 6300 ggagcttgca tgttggtttc tacgcagaac tgagccggtt aggcagataa tttccattga 6360 gaactgagcc atgtgcacct tccccccaac acggtgagcg acggggcaac ggagtgatcc 6420 acatgggact tttaaacatc atccgtcgga tggcgttgcg agagaagcag tcgatccgtg 6480 agatcagccg acgcaccggg caggcgcgca acacgatcgc aaagtatttg aacgcaggta 6540 caatcgagcc gacgttcacg gtaccggaac gaccaagcaa gctagcttag taaagccctc 6600 gctagatttt aatgcggatg ttgcgattac ttcgccaact attgcgataa caagaaaaag 6660 ccagcctttc atgatatatc tcccaatttg tgtagggctt attatgcacg cttaaaaata 6720 ataaaagcag acttgacctg atagtttggc tgtgagcaat tatgtgctta gtgcatctaa 6780 cgcttgagtt aagccgcgcc gcgaagcggc gtcggcttga acgaattgtt agacattatt 6840 tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg 6900 cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga 6960 cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg 7020 cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct 7080 catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa 7140 atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa 7200 cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct 7260 cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag 7320 ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga 7380 gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc 7440 gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct 7500 tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat 7560 ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt 7620 ccctcatgat gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc 7680 tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca 7740 tagactgtac cccaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac 7800 caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt 7860 acagcttacg aaccgaacag gcttatgtcc actgggttcg tgccttcatc cgtttccacg 7920 gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga ggcatttctg tcctggctgg 7980 cgaacgagcg caaggtttcg gtctccacgc atcgtcaggc attggcggcc ttgctgttct 8040 tctacggcaa gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc 8100 gtccgggcgc ttgccggtgg tgctgacccc ggatgaagtc tctagagctc tagagggttc 8160 gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc ccagcttctg tatggaacgg 8220 gcatgcggat cagtgagggt ttgcaactgc gggtcaagga tctggatttc gatcacggca 8280 cgatcatcgt gcgggagggc aagggctcca aggatcgggc cttgatgtta cccgagagct 8340 tggcacccag cctgcgcgag cagggatcga tccaacccct ccgctgctat agtgcagtcg 8400 gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta 8460 cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca 8520 taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg 8580 ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg 8640 ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc 8700 gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag 8760 tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca 8820 tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc 8880 cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa 8940 tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc 9000 cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag 9060 gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg 9120 cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg 9180 aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac 9240 aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga 9300 ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg 9360 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt 9420 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag 9480 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg 9540 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc 9600 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc 9660 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt 9720 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc 9780 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc 9840 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg 9900 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc 9960 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg 10020 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc 10080 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag 10140 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga 10200 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa 10260 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg 10320 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc 10380 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga 10440 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg 10500 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc 10560 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc 10620 atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc 10680 cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca 10740 agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc 10800 gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga 10860 cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg 10920 tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc 10980 cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc 11040 ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt 11100 gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca 11160 gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg 11220 tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag 11280 ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc 11340 tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc 11400 cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc 11460 cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc 11520 cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc 11580 ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg 11640 caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca 11700 aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata gcacgtacat 11760 tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta 11820 cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc 11880 cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact 11940 gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc 12000 cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg 12060 cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg 12120 gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 12180 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 12240 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac 12300 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag 12360 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 12420 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 12480 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 12540 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 12600 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 12660 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 12720 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 12780 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 12840 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 12900 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 12960 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 13020 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 13080 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 13140 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 13200 tccggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata 13260 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgcc 13305 <210> 34 <211> 13326 <212> DNA <213> Artificial <220> <223> pTVE503 <400> 34 agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60 cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120 acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180 cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240 cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300 ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360 tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420 aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480 agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agcctaatca 540 atagacataa tgtcaagcgt tgagccttta tgtgcttcac actgctcgac aacttcatca 600 atttttcttg aagcccatgg aaatcttggg ttgattaaga aaggacgtaa gtcaaatctg 660 ttgtcttctg gtgaatactg ttcagcatga taactaggag ttaaaacagt ttgcttgtgt 720 ctgttgatgg catagaagaa gaaaaatctt ttcacctttt cagatatttg acgaggtgtt 780 aactttgggg accattgatg aagaagtttt aagaacatag aataaggacc acatttttca 840 acctttctta ggtaaccaaa cacgcccaat tcttcatacg tcatccccat atctatctca 900 tccgattgaa cgtaatcttt agtcataggt tctaattctg cagttggtgt agcgtttaaa 960 aagtcattca agattggcat gttatattgt tttgatgcgt aggcaatgaa tcttttcaag 1020 tcagtttttg aaataccccc aatagggttg atatctgcgg aggagcagtc atattttgtt 1080 agatacccac gtaagcactc atcaacattt gcgctaccaa gtactaacaa tccacccgag 1140 tttgggatac cacgaaccca cggcaacagt tgcgcaaaaa gataagaaag aaccattctt 1200 agacgcgcct ggatgttttg taaagccaag ttctcgattt gagatccccc aaatattttg 1260 tatattggtt ttttgccagt ggctacttcg aataaggaca ccacactgga taccaatgag 1320 tccatcttta aatccacgtg gtaagatcca attgcattgg aaaggtcctt tgctctgttt 1380 cttgtctcct tggatgaatt ttccgtaccc atgaaacagg agtgaaatat ttttgaggct 1440 agatcctgtg gactgtctgg aatccaatca tcgccgctac gtgttatctt acgaacgtct 1500 ttgataactt gctcatttcc attttgagca gcgtcggtca ctaaacggca catagagtgg 1560 acaatcattg cagttgcaca agagtcaatg cccccagata agggaaggaa aaaccctgtt 1620 ccgttacaac gtcttaaata atcccacatc cagcaagcag gtcccagtgc aatttcttcc 1680 tcaggagagt gatagaaagg ctcgcggact tttgttggac acactgtagg atcaaatctg 1740 gaggtcatta aagccaattc tacaggaata tcaatacgct tgaactttat ttctgccaag 1800 gaggcttgta ggccacgaga catgacagct gcacgataac tcctcacctc ttctaggtcc 1860 acagtagcag taactacttc cacatcatct agcgaaaatt gtgaaccttg ggctacaatt 1920 gtaccattga tggcaattag tgcacagcca tcataatata atctgtcacc atcacaacct 1980 ctttgatttg catacaagta aacaccacca caacgtttag tggcatttaa aattaggtct 2040 aaccttttat ttaacttacg cagttcatga tgagaaccag atgagtttgt catgatttcc 2100 acaccatcta aagacatggc gatgtggggg gattgaggtg taaacaattc ttcacaagtt 2160 tctgtaccaa tgcatgtatc caatgaattt atcacagcgt ccccaaatgg cacaagtctc 2220 tggccggtaa ctttctgaat ctcaggtgga aggataaagt cctccaccac gccaggtttc 2280 atccaaggtg tgaaaaatct catttcccta tagttaccat cattagctaa ccaaatctta 2340 ggtcttatga acaatatctc accatccaag gataacaaac gacaattata acgaacattc 2400 ttgtgtagaa cgggcatacc aatgtcaagt attaatccat gggtttcttt attcttaatg 2460 atttgagcat acatttccca tgaatgaagg caaacgtcat tttctaaaaa atgatctaaa 2520 catccgtagc cagttatttc cagttctggg ccgacacgta acctggcacc cctctctttg 2580 gcaatcttaa tggactgtag gatacggtct ctattacctt caaaatctag ggcccattga 2640 ttcaagttgc atgtagctaa agtgataaga accttgcgct tcttcttggg atgtgccatg 2700 gttttggttt aataagaaga gaaaagagtt cttttgttat ggctgaagta atagagaaat 2760 gagctcgagt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag 2820 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct 2880 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat 2940 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat 3000 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg 3060 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc 3120 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca 3180 ccatgttgac gaagattttc ttcttgtcat tgagtcgtaa aagactctgt atgaactgtt 3240 cgccagtctt cacggcgagt tctgttagat cctcgatctg aatttttgac tccatgtatg 3300 gtgcatatgg cgcgccatat gcccgggccc tgtacagcgg ccgcgttaac gcgtatactc 3360 tagagcgatc gcccgggccg gccatttaaa tgaattcgag ctcggtaccc aaacgcggcc 3420 gcaagctata acttcgtata gcatacatta tacgaagtta ttcgactcta gaggatccca 3480 attcccatgc atggagtcaa agattcaaat agaggacact tctcgaactc ggccgtcgaa 3540 ctcggccgtc gagtacatgg tcgataagaa aaggcaattt gtagatgtta attcccatct 3600 tgaaagaaat atagtttaaa tatttattga taaaataaca agtcaggtat tatagtccaa 3660 gcaaaaacat aaatttattg atgcaagttt aaattcagaa atatttcaat aactgattat 3720 atcagctggt acattgccgt agatgaaaga ctgagtgcga tattatgtgt aatacataaa 3780 ttgatgatat agctagctta gctcatcggg ggatcctaga cgcgtgagat cagatctcgg 3840 tgacgggcag gaccggacgg ggcggtaccg gcaggctgaa gtccagctgc cagaaaccca 3900 cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc 3960 cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc 4020 tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc 4080 ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt 4140 tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga 4200 cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct 4260 gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga 4320 ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggtcca 4380 ttgttcttct ttactctttg tgtgactgag gtttggtcta gtgctttggt catctatata 4440 taatgataac aacaatgaga acaagctttg gagtgatcgg agggtctagg atacatgaga 4500 ttcaagtgga ctaggatcta caccgttgga ttttgagtgt ggatatgtgt gaggttaatt 4560 ttacttggta acggccacaa aggcctaagg agaggtgttg agacccttat cggcttgaac 4620 cgctggaata atgccacgtg gaagataatt ccatgaatct tatcgttatc tatgagtgaa 4680 attgtgtgat ggtggagtgg tgcttgctca ttttacttgc ctggtggact tggccctttc 4740 cttatgggga atttatattt tacttactat agagctttca tacctttttt ttaccttgga 4800 tttagttaat atataatggt atgattcatg aataaaaatg ggaaattttt gaatttgtac 4860 tgctaaatgc ataagattag gtgaaactgt ggaatatata tttttttcat ttaaaagcaa 4920 aatttgcctt ttactagaat tataaatata gaaaaatata taacattcaa ataaaaatga 4980 aaataagaac tttcaaaaaa cagaactatg tttaatgtgt aaagattagt cgcacatcaa 5040 gtcatctgtt acaatatgtt acaacaagtc ataagcccaa caaagttagc acgtctaaat 5100 aaactaaaga gtccacgaaa atattacaaa tcataagccc aacaaagtta ttgatcaaaa 5160 aaaaaaaacg cccaacaaag ctaaacaaag tccaaaaaaa acttctcaag tctccatctt 5220 cctttatgaa cattgaaaac tatacacaaa acaagtcaga taaatctctt tctgggcctg 5280 tcttcccaac ctcctacatc acttccctat cggattgaat gttttacttg taccttttcc 5340 gttgcaatga tattgatagt atgtttgtga aaactaatag ggttaacaat cgaagtcatg 5400 gaatatggat ttggtccaag attttccgag agctttctag tagaaagccc atcaccagaa 5460 atttactagt aaaataaatc accaattagg tttcttatta tgtgccaaat tcaatataat 5520 tatagaggat atttcaaatg aaaacgtatg aatgttatta gtaaatggtc aggtaagaca 5580 ttaaaaaaat cctacgtcag atattcaact ttaaaaattc gatcagtgtg gaattgtaca 5640 aaaatttggg atctactata tatatataat gctttacaac acttggattt ttttttggag 5700 gctggaattt ttaatctaca tatttgtttt ggccatgcac caactcattg tttagtgtaa 5760 tactttgatt ttgtcaaata tatgtgttcg tgtatatttg tataagaatt tctttgacca 5820 tatacacaca cacatatata tatatatata tatattatat atcatgcact tttaattgaa 5880 aaaataatat atatatatat agtgcatttt ttctaacaac catatatgtt gcgattgatc 5940 tgcaaaaata ctgctagagt aatgaaaaat ataatctatt gctgaaatta tctcagatgt 6000 taagattttc ttaaagtaaa ttctttcaaa ttttagctaa aagtcttgta ataactaaag 6060 aataatacac aatctcgacc acggaaaaaa aacacataat aaatttgaat ttcgaccgcg 6120 gtacccggaa ttgggttata attacctcag gtcgaggaat taattcggta cgtacctaat 6180 aacttcgtat agcatacatt atacgaagtt atatggatct cgaggcatta cggcattacg 6240 gcactcgcga gggtcccaat tcgagcatgg agccatttac aattgaatat atcctgccgc 6300 cgctgccgct ttgcacccgg tggagcttgc atgttggttt ctacgcagaa ctgagccggt 6360 taggcagata atttccattg agaactgagc catgtgcacc ttccccccaa cacggtgagc 6420 gacggggcaa cggagtgatc cacatgggac ttttaaacat catccgtcgg atggcgttgc 6480 gagagaagca gtcgatccgt gagatcagcc gacgcaccgg gcaggcgcgc aacacgatcg 6540 caaagtattt gaacgcaggt acaatcgagc cgacgttcac ggtaccggaa cgaccaagca 6600 agctagctta gtaaagccct cgctagattt taatgcggat gttgcgatta cttcgccaac 6660 tattgcgata acaagaaaaa gccagccttt catgatatat ctcccaattt gtgtagggct 6720 tattatgcac gcttaaaaat aataaaagca gacttgacct gatagtttgg ctgtgagcaa 6780 ttatgtgctt agtgcatcta acgcttgagt taagccgcgc cgcgaagcgg cgtcggcttg 6840 aacgaattgt tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga 6900 caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag 6960 cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt 7020 cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca 7080 acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta 7140 aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg 7200 ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat 7260 caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc 7320 caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa 7380 tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa 7440 ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca 7500 aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg 7560 ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg 7620 atacttcggc gatcaccgct tccctcatga tgtttaactt tgttttaggg cgactgccct 7680 gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt 7740 gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga 7800 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg 7860 agcgcatacg ctacttgcat tacagcttac gaaccgaaca ggcttatgtc cactgggttc 7920 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg 7980 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg 8040 cattggcggc cttgctgttc ttctacggca agtgctgtgc acggatctgc cctggcttca 8100 ggagatcgga agacctcggc cgtccgggcg cttgccggtg gtgctgaccc cggatgaagt 8160 ctctagagct ctagagggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg 8220 cccagcttct gtatggaacg ggcatgcgga tcagtgaggg tttgcaactg cgggtcaagg 8280 atctggattt cgatcacggc acgatcatcg tgcgggaggg caagggctcc aaggatcggg 8340 ccttgatgtt acccgagagc ttggcaccca gcctgcgcga gcagggatcg atccaacccc 8400 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca 8460 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc 8520 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg 8580 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc 8640 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg 8700 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac 8760 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc 8820 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc 8880 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg 8940 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg 9000 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc 9060 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc 9120 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca 9180 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg 9240 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt 9300 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc 9360 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct 9420 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg 9480 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt 9540 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc 9600 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc 9660 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg 9720 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag 9780 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct 9840 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac 9900 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga 9960 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag 10020 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc 10080 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc 10140 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt 10200 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg 10260 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg 10320 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag 10380 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca 10440 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa 10500 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc 10560 gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa 10620 tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga 10680 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc 10740 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc 10800 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt 10860 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc 10920 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc 10980 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga 11040 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa 11100 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg 11160 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac 11220 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc 11280 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga 11340 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc 11400 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct 11460 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat 11520 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct 11580 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc 11640 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg 11700 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt 11760 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta 11820 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa 11880 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac 11940 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc 12000 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc 12060 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc 12120 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg 12180 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 12240 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 12300 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc 12360 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 12420 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 12480 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 12540 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 12600 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 12660 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 12720 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 12780 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 12840 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 12900 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 12960 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 13020 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 13080 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 13140 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 13200 aaaaaaagga tctcaagaag atccggaaaa cgcaagcgca aagagaaagc aggtagcttg 13260 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga 13320 attgcc 13326

Claims

(a) 하기 i, ii 및 iii의 작동 가능하게 연결된 DNA 단편들을 포함하는 키메릭 유전자를 식물의 세포에 도입시켜 트랜스제닉 세포를 수득하는 단계:

i. 식물-발현성 프로모터,

ii. 서열식별번호: 2의 아미노산 서열을 포함하는 효소를 암호화하는 DNA 부위, 및

iii. 전사 종결 및 폴리아데닐화에 관여하는 3' 단부 부위;

(b) 상기 트랜스제닉 세포를 재생시켜 트랜스제닉 식물군을 수득하는 단계; 및

(c) 상기 트랜스제닉 식물군으로부터, 증가된 스트레스 내성을 나타내는 식물을 선택하거나, 또는 비 트랜스제닉 식물에 비해 스트레스 조건 하에서 감소된 반응성 산소 종 수준을 나타내거나 또는 높은 NADH 수준을 유지시키는 식물을 선택하는 단계

를 포함하는, 스트레스 내성이 증가된 식물의 수득 방법.
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제 1 항에 있어서,

상기 DNA 부위가 서열식별번호: 1의 뉴클레오타이드 서열을 포함하는, 방법.
제 1 항 또는 제 3 항에 있어서,

상기 식물을 또 다른 식물과 교배하는 단계를 추가로 포함하는 방법.
제 1 항 또는 제 3 항에 기재된 키메릭 유전자.
제 5 항에 따른 키메릭 유전자를 포함하는 식물 세포.
제 5 항에 따른 키메릭 유전자를 포함하는 식물.
제 7 항에 있어서,

목화, 배추과 식물, 평지 유지종자, 밀, 옥수수, 보리, 해바라기, 벼, 귀리, 사탕수수, 대두, 야채, 치커리, 상추, 토마토, 담배, 감자, 사탕무, 파파야, 파인애플, 망고 및 아라비도프시스 탈리아나(Arabidopsis thaliana) 중에서 선택되는 식물.
제 7 항에 있어서,

스트레스 조건 하에서 키메릭 유전자를 포함하지 않는 식물보다 더 낮은 수준의 반응성 산소 종을 가짐을 추가의 특징으로 하는 식물.
제 5 항에 따른 키메릭 유전자를 포함하는 종자.
제 5 항에 따른 키메릭 유전자를 식물에 도입하여 식물의 스트레스 내성을 증가시키는 방법.
제 5 항에 따른 키메릭 유전자를 식물 또는 식물 세포에 도입하여, 스트레스 조건 하에서 식물 또는 식물 세포 중의 반응성 산소 종 수준을 감소시키거나 또는 스트레스 조건 하에서 식물 또는 식물 세포 중의 NAD 수준을 유지시키는 방법.
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