KR101297712B1 - Biocide/disinfection agent containing hypochlorous acid water and soy protein - Google Patents

Abstract

Sodium hypochlorite, a conventional disinfectant disinfectant, generates chlorine gas when it is vaporized. Various papers and experiments show that chlorine gas can cause allergies and asthma. . On the other hand, hypochlorous water has little chlorine gas generation, which can guarantee worker safety. The present invention has a main component of hypochlorous acid water, which is safe for the human body. Hypochlorite water is obtained by the electrolysis of hydrochloric acid or saline, and refers to an aqueous solution containing hypochlorous acid as an active ingredient. It contains strong acidic hypochlorous acid solution (aqueous solution obtained from the anode side by electrolytic solution of sodium chloride of 0.2% or less in a diaphragm electrolytic cell composed of a positive electrode and a negative electrode separated by a diaphragm) and an unacidic hypochlorous acid solution (2-6% hydrochloric acid). (Aqueous solution obtained by electrolysis in a non-diaphragm electrolyzer comprised of the positive electrode and the negative electrode which are not separated by a diaphragm). The present invention relates to a method for optimizing sterilization, disinfection using hypochlorous acid and treatment by a suitable concentration for each site. In addition, hypochlorous acid and vegetable soybean protein are mixed to maximize the effect of sterilization and disinfection. It is also an object of the present invention to devise an optimization method of concentration difference, treatment place, sterilization and disinfection method for the target product using hypochlorous acid water.
The present invention relates to a disinfectant disinfectant and a method for disinfecting the target product using hypochlorous acid water, and more particularly, to increase the sterilizing power and disinfecting power of a chemical disinfectant whose component is hypochlorite water. Available places include water purification plants, sewage treatment plants, hospital operating rooms and medical equipment, food / beverage plant disinfection facilities, vegetables, cooling towers, chicken processing plants and meat plants, farms / fish farms, lakes, ponds, fountains, swimming pools, baths, and wastes. . Almost complete elimination of food poisoning bacteria, particularly Bacillus cereus, pathogenic E. coli and their spores, which are present in various places, can also be greatly reduced, and the use of harmful chemicals in existing chemical fungicides can be greatly reduced. Residual problem can be solved.
The present invention is a variety of bacteria, such as water purification plant, sewage treatment plant, hospital operating room and medical equipment, food / beverage plant disinfection equipment, vegetables, cooling towers, chicken processing plant and meat factory, aquaculture farm / fish farm, lake, pond, fountain, swimming pool, bath, waste The present invention relates to a treatment agent composition for inhibiting, and more particularly, a carbamate-based compound in a liquid form, and in particular, a variety of excellent mold inhibiting agents that cause filamentous bulking without affecting general bacteria useful for various treatments. It relates to a treating agent composition.

Description

Bactericidal disinfectant containing soy protein in hypochlorous acid {Biocide / disinfection agent containing hypochlorous acid water and soy protein}

The present invention relates to a bactericidal disinfectant prepared by adding soy protein to hypochlorous acid water.

The present invention relates to hypochlorous acid water produced by a hypochlorous acid production system for effectively sterilizing objects hygienically, ranging from drinking water, such as drinking water to food, medicine.

In particular, the present invention relates to hypochlorous acid water produced by the production system of hypochlorous acid (HCl), which has a high toxicity and sterilization effect using an electrolytic cell without a diaphragm.

Oxidizing biocide has a strong oxidizing power to oxidize microbial cell proteins to sterilize. Chlorine (or a combination of chlorine and bromine) and monochloramine are mainly used. Chlorine and bromine are strong oxidants (acutely toxic) with short half-lives, but they come into contact with halogens to form halogenated by-products. Other oxidizing fungicides include ozone, ultraviolet light, hydrogen peroxide and peracetic acid. Ozone and ultraviolet light require pretreatment of supplemented water or additives, which are less harmful than halogenated fungicides, but are expensive and cannot be applied in all situations.

Chlorine sterilizers most commonly used as cooling water disinfectants include chlorine gas, calcium hypochlorite, sodium hypochlorite, and the like, although there are differences in form, there is no difference in the principle of action of these chlorine disinfectants. As chlorine disinfectant is shown in the following equation 1 and the equation 2, can from hypochlorous acid (HOCl) and the difference of chloride ion (OCl ^-), and generate a, sterilizing the OCl of HOCl ^- the superior effect of about 50 to 100 times the have.

[Formula 1]

Chlorination: Cl ₂ + H ₂ O → HCl + HOCl

[Formula 2]

HOCl → H + OCl ^-

Nonoxidizing biocide exerts bactericidal activity by inhibiting the metabolism of microorganisms. Non-oxidizing fungicides include isothiazolones, methylene bisthiocyanate, glutaraldehyde, and quaternary ammonium. It has the characteristic that it is. Most of these substances are acutely toxic and most are not readily biodegradable, although some may be hydrolyzed or degraded by other mechanisms.

Accordingly, the present inventors have studied to obtain a remarkably excellent bactericidal effect while excluding the above-mentioned risks, hypochlorous acid (HOCl) is already acute toxicity test, eye irritation test, skin primary irritation test, skin cumulative irritation test, sensory test It is determined that there is no toxicity through cytotoxicity test, mutagenicity test, and the like, and thus, hypochlorous acid is selected and the hypochlorous acid water obtained by the conventional manufacturing method and apparatus for efficient, economical and safe use in the industrial purpose is provided.

The present inventors found that various types of carbamate-based compounds are inexpensive as inhibitors of microbial enzymatic activity, mainly act on filamentous microorganisms, and are chemically unstable and quickly metabolized in the body, thus toxic to human body compared to other antibacterial agents. The low and minimum inhibitory concentration (MIC) of filamentous microorganisms (MIC) was found to be a great effect on filamentous fungi suppression even with the addition of small amounts of 4-10 mg / l. The present invention was completed by recognizing that it is included in soy protein.

Carbamate-based fungicides, along with organophosphorus fungicides, are one of the major organic synthetic fungicides. The carbamate compound is composed of four elements, namely carbon (C), hydrogen (H), oxygen (O), and nitrogen (N), which is a source of environmental pollution. It does not contain This type of fungicide has a long history and was first discovered as a plant ingredient.

According to the present invention, the existing disinfectants such as sodium hypochlorite, hydrogen peroxide, benzalkonium chloride, formaldehyde, glutaraldehyde and the like are highly toxic and dangerous, but according to the present invention, acute toxicity test, eye irritation By selecting hypochlorous acid that has been reported to be nontoxic through experiment, primary skin irritation test, skin cumulative irritation test, sensory test, cytotoxicity test, mutagenicity test, etc. In addition, hypochlorous acid water obtained in an efficient and economic manner is provided.

The present invention provides a bactericidal disinfectant prepared by adding soy protein in an amount of 4 to 10 mg / L to 0.5 ppm to 2,000 ppm of hypochlorous acid water.

Hypochlorite water contained in the disinfectant disinfectant of the present invention is an aqueous solution obtained by electrolyzing hydrochloric acid in a membrane-free electrolytic cell composed of an anode and a cathode which are not separated by a diaphragm, and an aqueous solution having a hypochlorous acid (HOCl) content of 0.5 ppm to 2,000 ppm. to be.

In addition, the soy protein contained in the bactericidal disinfectant of the present invention may be a protein isolated from soybean, preferably is a soy protein isolated. Isolated soy protein is obtained by extracting and separating soybean protein by solubilizing soybean protein with water or alkaline solution after removing fat component from soybean, and is a vegetable protein source from which most carbohydrates and fats are removed. The soy protein used in the present invention may be used in solution or in powder form, commercially available one, or may be directly separated from soybean by a known method. An example of a method of preparing a soy protein solution is a mixing ratio of soy protein and water may be mixed in a weight ratio of 5 to 10:90 to 100, preferably 5: 100 by weight, and in the present invention, commercially available liquid soy protein is 70 It is possible to use a product prepared by continuously stirring and dissolving in purified water heated to ˜80 ° C., aging for 10 to 20 minutes, and cooling.

When the amount of hypochlorous acid in the disinfectant disinfectant of the present invention is 0.5ppm or less, the lack of bactericidal power is the most problematic, and it is difficult to expect the killing effect of the initial microorganisms or bacteria, and when it is 2,000ppm or more, excessive bleaching occurs with the occurrence of strong specific odor. The action can lead to discoloration or appearance deterioration of the object surface of sterilization / disinfection.

Soy protein in the disinfectant disinfectant of the present invention is preferably mixed in an amount of 4 ~ 10mg / L, it is difficult to expect the killing effect of the initial microorganism or bacteria when less than 4mg / L, if more than 10mg / L increased protein content Insufficient sterilization / disinfection effect on control filamentous fungi.

Sodium hypochlorite, a conventional disinfectant disinfectant, generates chlorine gas when it is vaporized. Various papers and experiments show that chlorine gas can cause allergies and asthma. . On the other hand, hypochlorous water has little chlorine gas generation, which can guarantee worker safety. The present invention has a main component of hypochlorous acid water, which is safe for the human body. Hypochlorite water is obtained by the electrolysis of hydrochloric acid or saline, and refers to an aqueous solution containing hypochlorous acid as an active ingredient. It contains strong acidic hypochlorous acid solution (aqueous solution obtained from the anode side by electrolytic solution of sodium chloride of 0.2% or less in a diaphragm electrolytic cell composed of a positive electrode and a negative electrode separated by a diaphragm) and an unacidic hypochlorous acid solution (2-6% hydrochloric acid). (Aqueous solution obtained by electrolysis in a non-diaphragm electrolyzer comprised of the positive electrode and the negative electrode which are not separated by a diaphragm). The present invention relates to a method for optimizing sterilization, disinfection using hypochlorous acid and treatment by a suitable concentration for each site. In addition, hypochlorous acid and vegetable soybean protein are mixed to maximize the effect of sterilization and disinfection. It is also an object of the present invention to devise an optimization method of concentration difference, treatment place, sterilization and disinfection method for the target product using hypochlorite water.

The present invention relates to a disinfectant disinfectant and a method for disinfecting the target product using hypochlorous acid water, and more particularly, to increase the sterilizing power and disinfecting power of a chemical disinfectant whose component is hypochlorite water. Available places include water purification plants, sewage treatment plants, hospital operating rooms and medical equipment, food / beverage plant disinfection facilities, vegetables, cooling towers, chicken processing plants and meat plants, farms / fish farms, lakes, ponds, fountains, swimming pools, baths, and wastes. . It is possible to eliminate almost all food poisoning bacteria, especially Bacillus cereus, pathogenic Escherichia coli and their spores, which are present in various places, and also greatly reduce the use of harmful components in existing chemical fungicides. Residual problem can be solved.

The present invention is a variety of bacteria, such as water purification plant, sewage treatment plant, hospital operating room and medical equipment, food / beverage plant disinfection equipment, vegetables, cooling towers, chicken processing plant and meat factory, aquaculture farm / fish farm, lake, pond, fountain, swimming pool, bath, waste The present invention relates to a treatment agent composition for inhibiting, and more particularly, a carbamate-based compound in a liquid form, and in particular, a variety of excellent mold inhibiting agents that cause filamentous bulking without affecting general bacteria useful for various treatments. It relates to a treating agent composition.

Examples of the microbial agent having an antimicrobial / antibacterial effect including soy protein include 0.5 to 3 parts by weight of two or more bacteria exemplified above in 100 parts by weight of a liquid culture medium, and 8.2 to 14.2 parts by weight of other microorganisms for promoting growth (nutrient composition). Parts, 3 to 4 parts by weight of glucose, 5 to 10 parts by weight of soy flour, 0.0001 to 0.0005 parts by weight of biotin, 0.1 to 0.2 parts by weight of potassium phosphate, 0.05 to 0.1 parts by weight of calcium carbonate, 0.001 to 0.005 parts by weight of iron sulfate, water 86.6 It consists of 90.8 weight part. Such microbial preparations are examples of microbial preparations having excellent disease / pest disinfection control and soybean growth promoting effects including soy protein.

The microorganism that can inhibit the growth by applying the soybean protein is not limited to this, Salinibacterium sp., Aurantimonas sp., Spingomonas sp. , Porphyrobacter tepidarius, Chryseobacterium sp., Hydrogenphagasp., Aeromonas hydrophila, Bacillus megaterium ), Micrococcus luteus, Pseudomonas aeruginosa, Bacillus pumilus, Brevundimonas vesicularis, Hydrogenpaga sp. Staphylococcus warner, Roseomonas mucosa, Staphylococcus epidermidis, Axidoborax delafildi Acidovorax delafieldii), Aeromicrobium sp., Microbacterium oxydans, Pedobacter sp., Or variants thereof.

The present invention is described in more detail with reference to the following Examples, but the present invention is not limited thereto.

Example  One

950 g of 50 ppm hypochlorous acid and 50 g of 10 ppm soy protein solution were mixed to prepare a bactericidal disinfectant and tested for bactericidal effect. The table below shows the results of testing the disinfectant effect of the disinfectant disinfectant mixed with hypochlorous acid water and soy protein solution.The disinfectant disinfectant was diluted with water and the sterilization effect was recorded for each time period. It was confirmed that the comma was maintained in a dead state afterwards.

Table 1: Comma of Bactericidal Disinfectants bacillus Bactericidal effect on (Comma fungus)

(+) ........ Survival (-) ........ Destruction

 ㆍ Bacteria used: Vibrio Comma Inaba

The phenolic coefficients of the bactericidal disinfectants of the present invention are shown in Table 2 below. These phenolic coefficients require a method of comparing the effects of various disinfectants and are a parameter for such comparison. The phenolic coefficient is obtained by selecting several pathogens to determine the disinfectant power of phenol as 1 and the concentration of the test disinfectant that is killed within 10 minutes without killing within 5 minutes. The relative index for phenol is 100. This disinfectant can be said to have 100 times stronger antiseptic power than phenol.

Table 2: Phenol Coefficients of Bactericidal Disinfectants

ㆍ Test method: described in KFDA and KP

 Test bacteria: Typhoid bacillus

Example 2

In the present Example, the amount of hypochlorous acid was tested for various purposes by varying the amount of hypochlorous acid, and the amount showing the excellent effect was confirmed, and the appropriate amount is shown in a table. Table 3 calculates the amount of use criteria for each field in consideration of the phenol coefficient of Table 2.

TABLE 3

Example 3

This example shows the test results for some microorganisms in Table 4. In these tests, the test solution for the bactericidal concentration test follows the usage and capacity of the standards and specifications, and the basic operation for microbial experiments is in accordance with 8. Microbiological Test Method in Article 7. General Test Methods of the Food Code.

Table 4

Example 4

This example is an example of testing the titer of an antimicrobial agent. The titer of an antimicrobial agent is determined by measuring the minimum drug concentration that inhibits growth, known as the minimum inhibitory concentration (MIC). In order to measure the MIC of a given drug, a series of test tubes containing a medium containing different concentrations of antimicrobial agents to be tested were prepared and inoculated with the test strain. After incubation, visible growth based on turbidity of the tubes was examined. MIC is the minimum concentration of a drug that completely inhibits the growth of a given microorganism. This simple and effective method is also known as the tube dilution technique.

The MIC of the antimicrobial agent cannot be absolute because the MIC depends on the characteristics of the strain used, the inoculation amount, the composition of the medium, the culture time and temperature, pH, aeration, and the like. But by thoroughly standardizing all conditions, you can compare different types of antimicrobial agents to determine what works best for a particular microorganism. Table 5 shows the results of titer tests of antimicrobial agents.

Table 5

Example 5

This example confirms the bactericidal power of microorganisms using hypochlorite water containing soy protein and hypochlorite water disinfectant containing no soy protein, and the results are shown in Table 6 below.

Table 6

-*: ATCC43895, **: IFO3313, ***: ATCC6633

 -30 minutes for bacterial follicles and 1 minute for others

 Hypochlorous acid alone: pH 6.2, effective chlorine concentration 10ppm

 Hypochlorite + protein solution: pH 6.5, effective chlorine concentration 10ppm, protein concentration 5ppm

The effective chlorine concentration is the concentration of hypochlorous acid contained in the disinfectant disinfectant, and when the protein is contained, it can be seen that the bactericidal effect is excellent in apo, mold, yeast and the like.

Claims (2)

A bactericidal disinfectant prepared by adding soy protein in an amount of 4 to 10 mg / L to 0.5 ppm to 2,000 ppm of hypochlorous acid water. The disinfectant disinfectant according to claim 1, wherein the hypochlorous acid is obtained by electrolyzing hydrochloric acid in a membrane-free electrolytic cell composed of an anode and a cathode.