KR101176305B1 - Composition for prevention of influenza virus comprising extracts of True Rhus, air filter comprising the composition and air cleaning device comprising the filter - Google Patents

Abstract

The present invention relates to a composition for the prevention of influenza virus infection comprising a rhododendron extract, a gas phase filter comprising the composition, and an air purifier including the filter, wherein the composition including the rhododendron extract has excellent anti-influenza activity. The filter coated with the composition can be effectively used for the prevention of viral infections and can be effectively used for the prevention of influenza virus infection because it can effectively remove the influenza virus in the air.

Description

Composition for prevention of influenza virus comprising extracts of True Rhus, air filter comprising the composition and air cleaning device configuring the filter}

The present invention relates to a composition for the prevention of influenza virus infection comprising a red tree extract, a gas phase filter comprising the composition, and an air cleaner having the filter.

Influenza is an infectious disease commonly called flu, or the influenza virus of the Oxomyxovirus family. Common symptoms include chills, fever, sore throat, myalgia, headache, cough, helplessness and discomfort, and mild symptoms like fever and cough are most easily seen, but can often cause fatal complications.

Influenza causes pandemic flu, which kills thousands to tens of thousands of people every season, and in the global pandemic that has occurred over the years, about one million people have died. In the 20th century, there were three global epidemics of the flu caused by new strains of influenza, killing tens of millions of people. These strains often occur when infections occur from other animals to humans, and when a species whose main host is human receives genes from a species whose main host is another animal. The emergence of H5N1 in Asia in 1990 brought great attention to the global flu epidemic, but the species did not change to specialize in human-to-human infections. In April 2009, a variant of H1N1 emerged in Mexico, spreading to several countries.

Influenza is strictly divided into three of the five genus included in the RNA virus Orthomyxoviridae, influenza A, influenza B, and influenza C.

Influenza A includes only one species, the influenza A virus. Wild aquatic birds act as natural hosts for many types of influenza A. Type A can cause a major epidemic through transmission of species and is the most toxic of influenza. Type A is further divided into several serotypes (subtypes) depending on whether the antibody responds. The subtypes known to cause flu in humans are listed in descending order by number of deaths during the epidemic. First, H1N1 caused the Spanish flu epidemic of 1918 and the worldwide flu epidemic of 2009. H2N2 caused Asian flu in 1957, and H3N2 was the subtype of Hong Kong flu in 1968. In addition, subtypes of influenza A include H5N1, H7N7, H1N2, H9N2, H7N2, H7N3, and H10N7.

Among them, H1N1 is described in more detail. Influenza virus type A H1N1 subtype (English: Influenza A virus subtype H1N1) or H1N1 is a subtype of influenza A, which is the most common type of influenza in humans. In addition to humans, pigs and birds can also be infected.

H1N1 caused pandemic flu, called the Spanish flu, and between 1918 and 1919 killed between 50 and 100 million people. In 2005, the genome of H1N1 was published in Science, the virus of which produced the Spanish flu in 1918, and only 25 to 30 of the 4,400 amino acids were mutated compared to the current one. Low-pathogenic H1N1 is now widely distributed around the world, and in 2006 almost half of human infections were reported.

However, the number of deaths from the H1N1 virus has increased worldwide since March 2009. The virus was called Swine Flu at the outset, but was later eliminated by the swine flu. (new influenza) or the new influenza A (H1N1) virus, and on June 11 of the same year, the World Health Organization raised the pandemic warning level of H1N1 influenza.

As of September 16, 2009, more than 10,000 people have been infected, 8 have been severely hospitalized, and 14 have been reported to have died from acute complications or acute complications.

Influenza B, on the other hand, includes one species that is an influenza B virus. Type B is almost susceptible to humans, and is rarer than type A. Other animals suspected of having type B susceptibility are seals and ferrets. This species has two to three times lower mutation rate than type A, and there is only one subtype with low genetic diversity. Because of this low antigenic diversity, type B has a small host range and very few epidemics.

Influenza C also includes one species that is influenza C virus. Type C is susceptible to humans, dogs, and pigs, but usually only mild symptoms in children. It appears less frequently than type A and type B.

Drugs conventionally used to treat influenza include oseltamivir (trade name: Tamiflu), zanamivir (trade name: Relenza), feramivir (en: Peramivir), and amantadine (en: Amantadine). Oseltamivir, called Tamiflu, is currently used primarily for the treatment of H1N1 influenza type A. Tamiflu is the world's only monoclonal avian influenza (AI) treatment. It is an antiviral agent that has a therapeutic effect by blocking the function of the enzyme to multiply the virus, and it takes effect within 48 hours after symptoms occur. The main therapeutic effects are a reduction in the worsening of flu symptoms, a decrease in the occurrence of secondary complications such as bronchitis or pneumonia, and a decrease in the incubation period for flu. It is also used as a treatment for influenza A and B. Janamivir is also called Zanamivir and is trade name Relenza. It acts as a neuraminidase inhibitor and is used to treat influenza A and B.

However, oseltamir has a side effect of severe vomiting and zanamivir has a high antiviral effect but low bioavailability and fast discharge from the kidney.

Most anti-influenza drugs developed to date show side effects. Therefore, there is an urgent need to develop an anti-influenza composition effective for the prevention and treatment of influenza.

Influenza, on the other hand, is infected by inhaling virus-containing aerosols, which usually come into the air through coughing or sneezing. In addition, it can be transmitted from other individuals' feces, saliva, runny nose, feces and blood, but most of the transmission can be said to be a splash infection from aerosol inhalation. Such influenza can be inactivated by surfactants such as sunlight, disinfectant, soap, etc. to prevent infection, but it does not help to remove viruses contained in the air.

Therefore, if the virus in the air can be removed, it will be able to effectively prevent the influenza infection.

An air filter is a breathable member that filters contaminants in the gas passing through the filter to purify the gas passing through the filter. Such a weather filter is used in various places such as home appliances, vehicles, buildings, for example, may be used in air cleaners, air conditioners, dryers, cleaners, humidifiers, dehumidifiers, vehicle air conditioners, central air conditioning systems in buildings, and the like.

On the other hand, an air cleaner is a device for purifying contaminated air and converting it into fresh air. A plurality of filters are used in one air cleaner. In general, a pretreatment filter for removing large particles of dust and the like; A deodorizing filter for removing toxic substances such as odors, volatile organic compounds and formaldehyde; Hepa filter which is a high performance filter which removes fine dust; One or two or more intermediate filters installed on the front of the HEPA filter to protect the HEPA filter from dust may be used in one air cleaner.

The more such filters are used, the better the air purification performance can be, but the overall resistance to air circulation can be increased, so it is common to use a filter of three to five stages overall.

Recently, a functional filter capable of selectively removing more harmful substances to the human body using an air cleaner or replacing harmful substances with substances beneficial to the human body has been studied. Exemplary researches include high-performance micromaterial filters that can filter out finer materials for infants, or filters for sickhouse syndrome that can filter out harmful substances caused by sick house syndrome.

Sumac is a deciduous arborescent belonging to Asiatic Anacardiaceae, which is also known as Magnus. Its height is 7m and leaves are alternately made of 7 ~ 13 leafs of leaves, with serrates on the edges of the leaves and wings between the leaves and leaves. In August-September, pale yellow or white flowers bloom separately in males or females, or conical inflorescences bloom at the end of stems. Boiled young shoots in the spring to eat as herbs, people need to be careful because there are toxic ingredients. Parasitic aphids ( Melaphis chinensis ), which grow on parasitic redwoods, create pouch-like worms, which are called gallanders (五 培 子).

Thus, the inventors of the present invention in search of the anti-influenza composition in view of the above point that the red rose extract has excellent anti-influenza activity, so that the composition containing it as an active ingredient can be used for the prevention of influenza virus infection and the composition to the filter The present invention was completed by confirming that the influenza virus in the air can be effectively removed by coating.

It is an object of the present invention to provide a composition for the prevention of influenza virus infection comprising a rhododendron extract.

Another object of the present invention is to provide a gas phase filter comprising the composition.

Still another object of the present invention is to provide an air cleaner including the filter.

In accordance with one aspect of the present invention, there is provided a composition for preventing influenza virus infection, which comprises red rose extract as an active ingredient.

According to a preferred embodiment of the present invention, the composition may further comprise a ginkgo extract.

According to a preferred embodiment of the present invention, the composition can be used in quasi-drugs.

According to another aspect of the present invention, there is provided a gas phase filter comprising the composition.

According to another aspect of the present invention, an air cleaner including the gas phase filter is provided.

Hereinafter, the present invention will be described in detail.

The present invention provides a composition for the prevention of influenza virus infection comprising a rhododendron extract as an active ingredient.

According to another embodiment of the present invention, the composition may further comprise a ginkgo extract in addition to the Rhododendron extract.

As used herein, the term "prevention" means any action that inhibits or delays the influenza virus infection by use of the composition.

In the present invention, the influenza virus may be influenza A virus, influenza B virus, influenza C virus, preferably H1N1 influenza virus, more preferably a strain of influenza A virus H1N1 subtype. It may be a swine flu A (H1N1) virus.

The H1N1 influenza virus, officially known as Influenza A virus subtype H1N1, is the most common type of influenza in humans. In addition to humans, pigs and birds can be infected or infected. Specific types include A / PR / 8/34 (H1N1), A / WSN / 33 (H1N1), A / Bervig-Mission / 1/18 (rvH1N1), A / Singapore / 6/86 (H1N1).

Swine flu A (H1N1) viruses are viruses that caused the 2009 flu pandemic in 2009, including A / California / O4 / 09 and A / California / 7/2009.

In the present invention, the influenza virus can cause flu, cold, sore throat, bronchitis, pneumonia, and in particular can cause bird flu, swine flu or chlorine flu.

In the present invention, the type of sumac (Sumac) as Rusu javanica ( Rhus javanica ), Rhus Chinese chinensis ), but is not limited thereto.

In the present invention, the Rhododendron and the ginkgo can be purchased and used commercially, or may be used that is harvested or grown in nature.

In the present invention, as the site of the red tree may be used as a leaf, fruit, bark, root, etc., as well as the worm beetle made by the worm aphid (Melaphis chinensis) parasitic growing on the red tree, preferably May use, but is not limited to, leaves, bark or gall bladder.

In the present invention, the ginkgo may use a site such as a leaf, fruit, bark, root and the like, and preferably, a leaf may be used, but is not limited thereto.

In the present invention, the rhododendron or ginkgo extract can be applied to all methods that can be extracted from a simple extraction method to a fat-soluble component in the extraction method, pulverized for easy extraction and extracted with an extraction solvent, filtered and concentrated by You can get it.

Extraction solvents include water, ethanol, methanol, butanol, n-hexane, n-heptane or DMSO, and may be used as an extraction solvent by mixing two or more of them, but is not limited thereto. Those skilled in the art can appropriately select from known methods depending on the amount, extraction method, and the like. Extraction time and temperature can also be appropriately selected by those skilled in the art in consideration of extraction efficiency, extraction solvent and the like. Preferred extraction time is 10 minutes to 1 hour, and preferred extraction temperature is 40 to 100 ° C.

In addition, the extract extracted by the extraction method can be concentrated by distillation or the like after filtering by a known filtration method such as vacuum filtration. Such extraction, filtration and concentration methods are well known in the art and thus those skilled in the art can make appropriate selections to produce rhododendron or ginkgo extracts.

According to another embodiment of the present invention, the composition for preventing influenza virus infection of the present invention can be used in quasi-drugs.

That is, the composition of the present invention can be added to quasi-drug for the purpose of prevention of influenza virus infection. When the composition of the present invention is used as an quasi-drug additive, the composition may be added as it is or used together with other quasi-drug or quasi-drug components, and may be appropriately used according to a conventional method. The mixed amount of the active ingredient can be appropriately determined depending on the intended use.

Preferably, the quasi-drug composition may be used in the manufacture of filter coatings, hand washes, gagrins, antiseptic cleaners, shower foams, wet wipes, detergent soaps, humidifier fillers, masks or fragrances.

The present invention also provides a gas phase filter comprising a composition for preventing the influenza virus infection.

In the present invention, the term "meteorological filter" is a filter for removing microorganisms, dust and the like in the air to prevent the introduction of dangerous substances or contaminants such as microorganisms, dust from the outside, and to prevent secondary pollution by the filter Say. Therefore, the gas phase filter according to the present invention may be used as a gas phase filter for automobiles, a gas phase filter for home appliances, a gas phase filter for air conditioners, a gas filter for gas masks, a gas phase filter for air purifiers, or a gas phase filter for clean rooms, preferably air. It can be used in gas phase filter for purifier.

Furthermore, the present invention provides a method for producing a gas phase filter comprising the composition for preventing influenza virus infection, and the following describes a method for producing a gas phase filter including the composition for preventing influenza virus infection described above.

Method for producing a gas phase filter comprising a composition for preventing influenza virus infection of the present invention,

(a) preparing a composition for preventing influenza virus infection;

(b) coating the filter substrate with a composition for preventing influenza virus infection; And

(c) drying the coated filter,

In step (b),

Immersing a roller in the composition for preventing influenza virus infection, and then applying the composition to the filter substrate using the immersed roller;

Immersing a roller including the filter substrate in the composition for preventing influenza virus infection; And

Spraying the composition for preventing the influenza virus infection to the filter substrate is characterized in that it is carried out by one or more steps selected from the group consisting of.

As the filter substrate, metals, plastics, nonwovens, films and the like can be used. As a gas phase filter for an air cleaner, a highly porous nonwoven fabric is preferably used, but is not limited thereto.

Plastic materials include, but are not limited to, polypropylene, polyethylene, polyurethane, acrylic, PVC or polystyrene, and are preferably polypropylene.

Nonwoven fabric refers to a sheet-like cloth using fibers entangled by adhesive or self-adhesive strength by arranging fibers appropriately, and a paper base nonwoven fabric, a fiber base nonwoven fabric, etc., depending on the base material have. In addition, the nonwoven material may include, but is not limited to, rayon, tencel, polypropylene, and the like, and any material known in the art may be used as a conventional nonwoven material. Preferably polypropylene.

The filter substrate of the present invention makes a web of fibers (entangled in a lattice shape) without weaving or weaving a filament yarn which is produced by heating the plastic resin to a temperature near the melting point and melt spinning. It can be prepared as a filter for fibers of the non-woven fabric form, or by adding a blowing agent to a porous foam filter.

In order to prepare a gas phase filter, first, a composition for preventing influenza virus infection is prepared. The composition for preventing influenza virus infection may be used for preparing a gas phase filter in the form of a solution by dissolving or diluting with a predetermined solvent. The solvent may be water, ethanol, methanol, butanol or n-hexane, n-heptane, DMSO or a mixed solvent thereof.

The filter substrate is coated with the composition for preventing the influenza virus infection. A method of coating, in which an absorbent member such as a sponge is immersed in the composition, and then the roller is rolled on the filter substrate and dried to coat the coating, and the filter substrate is directly immersed in the composition and dried to coat. And a method of spraying and drying the composition on the filter substrate and coating the composition.

The coating process for the production of the filter of the invention can be applied not only to the already prepared filter substrate but also to the fiber form step prior to weaving the filter. In the case of the fibrous form, a filter process of dipping or spraying the filter weaving fibers with the composition of the present invention is first performed, and then the filter substrate is woven after the coating process.

When the composition of the present invention is to dry the filter coated by immersion or spray, can be dried at room temperature or hot air, the drying method may be used without limitation methods known in the art. Drying allows the composition of the present invention to adsorb well to the filter, so that the filter can have antimicrobial efficacy for a long time.

In addition to the composition of the present invention, the filter of the present invention may further include an existing antimicrobial agent, deodorant (for example, flavonoids, phytoncide, wood vinegar, plant extract, cyclodextrin, metal ions, titanium dioxide) or dust collecting filter. In addition, these components may be applied together or separately for each component, and the application order is not particularly limited.

In addition, the present invention provides an air cleaner including the gas phase filter.

Referring to the air purifier as an embodiment in which the gas phase filter of the present invention is used, as described above, a plurality of filters are used in the air purifier, and the gas phase filter including the composition for preventing influenza virus infection according to the present invention is functional. It may be used as a filter, and may be used as any filter included in other air cleaners.

There is no restriction on the air purifier to which the gaseous phase filter of the present invention is applied, and the air purifier in a home or office or the indoor purifying filter for a car may be an example, and a common component having such a conventional air purifier is included. It may include. Preferably, the air purifier of the present invention is provided by arranging the gas phase filter of the present invention between an air inlet and a jet port.

In addition, the present invention provides an air cleaning method using the air cleaner.

The composition comprising the red tree extract according to the present invention can be used for the prevention of influenza virus infection by having excellent anti-influenza activity and the filter coated with the composition can effectively remove the influenza virus in the air to prevent the influenza virus infection It can be usefully used.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. However, the following examples are merely to illustrate the present invention is not limited to the contents of the present invention.

Manufacturing example  1: Preparation of Rhododendron Extract

Redwood (Sumac) used in the present preparation was used after pulverizing the powder form after drying to obtain the extract of the present invention after collecting the leaf portion. 5 L of water was added to 1 kg of rhododendron, followed by extraction in a water bath at 80 ° C. for 12 hours, followed by filtration with a filter paper, followed by concentration.

Manufacturing example  2: Preparation of Ginkgo Extract

The ginkgo used in this preparation was dried and then ground to obtain an extract of the present invention after harvesting the leaf area and used in the form of powder. 5 l of water was added to 1 kg of ginkgo, extracted by pouring in a water bath at 80 ° C. for 12 hours, filtered through a filter paper, and concentrated to obtain a ginkgo water extract.

Manufacturing example  3: manufacture of the present invention the filter is coated with red wood extract

A filter was prepared by spray coating a solution obtained by diluting the Rhododendron extract prepared in Preparation Example 1 with water on a polypropylene filter substrate and drying at 140 ° C. for 4 minutes.

Manufacturing example  4: manufacture of the present invention filter coated with the extract of ginkgo and ginkgo

To prepare a filter by spray coating a solution obtained by diluting a mixture of the same amount of the Rhododendron extract prepared in Preparation Example 1 and the Ginkgo extract prepared in Preparation Example 2 on a polypropylene filter substrate with water and dried at 140 ℃ for 4 minutes. It was.

Experimental Example  1: Determination of Influenza Virus Killing Activity of Rhododendron and Ginkgo Biloba Extracts

Influenza virus killing activity of the Rhododendron extract and Ginkgo biloba extract obtained in Preparation Examples 1 and 2 of the present invention was measured as follows.

Influenza virus is a pandemic influenza A (H1N1) virus that was declared a pandemic in 2009 (World Health Organization, June 11, 2009) and the WHO standard strain H1N1 (A / California / O4 / 09). A / PR / 8/34 (H1N1) and A / WSN / 33 (H1N1) were used as viruses of other H1N1 types known as H1N1 types.

As a sample, a mixture of the redwood extract obtained in Preparation Example 1 alone, the ginkgo extract obtained in Preparation Example 2 alone, and the redwood extract and the ginkgo extract obtained in Preparation Examples 1 and 2 was used in the same amount. More specific experimental method was as follows.

First, the Mardine Darbine Canine Kidney (MDCK) cell line was inoculated at a concentration of 1.5 × 10 ⁶ cells / ml, followed by 37 ° C., 5% CO ₂ in a 6-well plate. Incubated for 24 hours in a concentration incubator. In addition, each single extract and mixture was diluted to 100 mg / ml (1 ×) in mesophilic water, which was diluted 10-fold to prepare a sample. 90 ul of each prepared sample was put in a 96 well plate and treated with 10 ul of influenza virus. After reacting for 10 minutes, the solution was diluted 10 times with PBS. Thereafter, the full grown MDCK cell line at the bottom of the culture dish was infected with 1 ml of the diluted solution for 1 hour. On the other hand, the "infection + non-administration" control (control, a group of cells not infected with any extract after infection with influenza virus) was incubated by inoculating the MDCK cell line after the reaction under the same conditions.

Thereafter, the medium was removed, and 2 x MEM medium containing 2 x agarose and 10 ug / ml trypsin was mixed at a weight ratio of 1: 1 and poured into 2 ml plates containing MDCK cell lines. After incubating the MDCK cell line in a 37 ° C. incubator for 2 days, 1 ml of 4% paraformaldehyde was added and fixed, and washed with water to remove agarose. Then, the number of plaques was measured by staining with crystal violet, and the number was calculated as plaque forming unit (pfu / ml). The results are shown in Table 1-3 below.

Titer of Swine Flu A (H1N1) (A / California / O4 / 09) After Each Sample Treatment sample Virus titer (pfu / ml) % Virus Inhibition Control group 1.9 x 10 ⁶ - Rhododendron Extract Not-detected 99.99 Bank extract 1.0 x 10 ² 99.99 Ginkgo extract + red tree extract Not-detected 99.99

 Titer of Influenza A / WSN / 33 (H1N1) After Each Sample Treatment sample Virus titer (pfu / ml) % Virus Inhibition Control group 3.4 x ^{10 8} - Rhododendron Extract Not-detected 99.99 Bank extract 7.0x10 ³ 99.99 Ginkgo extract + red tree extract Not-detected 99.99

 Titer of Influenza A / PR / 8/34 (H1N1) After Each Sample Treatment sample Virus titer (pfu / ml) % Virus Inhibition Control group 3.9 x ^{10 9} - Rhododendron Extract Not-detected 99.99 Bank extract 6.0 x 10 ² 99.99 Ginkgo extract + red tree extract Not-detected 99.99

Compared to the control group through the results of Table 1-3, the virus is completely killed when the mixture of the same amount of the Rhododendron extract and the Rhododendron extract and the Ginkgo biloba extract is completely killed. It was found that the mixture had excellent anti-influenza virus killing activity.

Experimental Example  2: measurement of influenza virus killing activity of the gas phase filter of the present invention

Influenza virus killing activity of the Rhus extract treated gas filter and Rhus extract and ginkgo extract treated gas filter obtained in Preparation Examples 3 and 4 of the present invention was measured as follows.

Influenza virus is a pandemic influenza A (H1N1) virus that was declared a pandemic in 2009 (World Health Organization, June 11, 2009) and the WHO standard strain H1N1 (A / California / O4 / 09). A / PR / 8/34 (H1N1) and A / WSN / 33 (H1N1) were used as viruses of other H1N1 types known as H1N1 types.

As a sample, a gas phase filter obtained in Production Examples 3 and 4 was used. As a control, a gas phase filter without any extract was used. More specific experimental method was as follows.

After collecting the filter medium with 2x2cm, apply a certain amount of virus solution to the filter, and absorb the filter medium for 10 minutes to react, and then put the medium (1ml) on the absorbed filter and shake for 10 minutes to wash out the virus. This sample was diluted 10 times to prepare a sample. 90 ul of each prepared sample was put in a 96 well plate and treated with 10 ul of influenza virus. After reacting for 10 minutes, the solution was diluted 10 times with PBS. Thereafter, the full grown MDCK cell line at the bottom of the culture dish was infected with 1 ml of the diluted solution for 1 hour. On the other hand, "infection + non-administration" control (control, a group of cells not infected with any extract after infection with influenza virus) was inoculated in the MDCK cell line after the reaction under the same conditions and incubated.

Thereafter, the medium was removed, and 2 x MEM medium containing 2 x agarose and 10 ug / ml trypsin was mixed at a weight ratio of 1: 1 and poured into 2 ml plates containing MDCK cell lines. After incubating the MDCK cell line in a 37 ° C. incubator for 2 days, 1 ml of 4% paraformaldehyde was added and fixed, and washed with water to remove agarose. Then, the number of plaques was measured by staining with crystal violet, and the number was calculated as plaque forming unit (pfu / ml) . The results are shown in Table 4-6 (see Equation 1).

 Titer of Swine Flu A (H1N1) (A / California / O4 / 09) After Each Sample Treatment sample Virus titer (pfu / ml) % Virus Inhibition Control group 1.9 x 10 ⁶ - Rhododendron Extract Treatment Weather Filter Not-detected 99.99 Mixture treatment vapor filter of rhododendron extract and ginkgo extract Not-detected 99.99

Titer of Influenza A / WSN / 33 (H1N1) After Each Sample Treatment sample Virus titer (pfu / ml) % Virus Inhibition Control group 3.4 x ^{10 8} - Rhododendron Extract Treatment Weather Filter Not-detected 99.99 Mixture Treatment of Rhododendron Extract and Ginkgo Extract Not-detected 99.99

Titer of Influenza A / PR / 8/34 (H1N1) After Each Sample Treatment sample Virus titer (pfu / ml) % Virus Inhibition Control group 3.9 x ^{10 9} - Rhododendron Extract Treatment Weather Filter Not-detected 99.99 Mixture Treatment of Rhododendron Extract and Ginkgo Extract Not-detected 99.99

Through the results of Tables 4 to 6, it was found that the gas filter of the present invention, the mixture treatment of the Rhododendron extract treatment and the Rhododendron extract and the ginkgo extract has excellent influenza virus killing activity.

Claims (18)

A composition for the prevention of influenza virus infection comprising a rhododendron extract as an active ingredient, wherein the influenza virus is A / California / O4 / 09. The composition of claim 1, wherein the composition further comprises a ginkgo extract. According to claim 1 or 2, wherein the extract is water, ethanol, methanol, butanol, n-hexane, n-heptane, DMSO or a mixed solvent thereof, characterized in that the composition for preventing influenza virus infection . The composition of claim 1, wherein the red tree is a leaf, fruit, bark, root, or gall bladder site. The method of claim 2, wherein the ginkgo is a leaf, fruit, bark or root region, characterized in that the composition for the prevention of influenza virus infection. delete delete delete delete delete The composition of claim 1, wherein the disease caused by influenza virus infection is flu, cold, sore throat, bronchitis, pneumonia, bird flu, swine flu, or goat flu. According to claim 1, wherein the composition is a composition for the prevention of influenza virus infection, characterized in that used in the manufacture of quasi-drugs. The composition for preventing influenza virus infection of claim 12, wherein the quasi-drug is a filter coating, a hand wash, a mouthwash, a disinfectant cleaner, a shower foam, a wet tissue, a detergent soap, a humidifier filler, a mask, or an air freshener. A gas phase filter comprising a composition for preventing the influenza virus infection of claim 1 or 2. (a) preparing a composition for preventing influenza virus infection of claim 1 or 2;
(b) coating the filter substrate with a composition for preventing influenza virus infection; And
(c) drying the coated filter,
The step (b)
Immersing a roller in the composition for preventing influenza virus infection, and then applying the composition to the filter substrate using the immersed roller;
Immersing a roller including the filter substrate in the composition for preventing influenza virus infection; And
Spraying the composition for preventing the influenza virus infection on the filter substrate, characterized in that carried out by one or more steps selected from the group consisting of, gas phase filter manufacturing method. The method of manufacturing a gas phase filter according to claim 15, wherein the filter base material is plastic or nonwoven fabric. The method for producing a gas phase filter according to claim 16, wherein the nonwoven fabric is a paper base nonwoven fabric or a fiber base nonwoven fabric. An air purifier comprising the gas phase filter of claim 14.