KR101174042B1 - Mixture extract having insecticidal and repellent effects and products using the same - Google Patents

Abstract

The present invention relates to a mixed extract having a pesticidal and repelling action of pests extracted from mugwort, moth, mountain and mulberry trees as an extraction solvent, and a product using the same. The mixed extract according to the present invention can be widely used to manufacture products such as pesticides, fragrances, detergents and cosmetics by showing excellent insecticidal and repelling effects against pests without abnormalities in the human body.

Wormwood, moth, Sancho, Mulberry, Pest, Insecticide, Repellent, Mixed Extract

Description

Mixture extract having insecticidal and repellent effects and products using the same}

The present invention relates to a mixed extract having a pesticidal and repelling action of pests extracted from mugwort, moth, mountain and mulberry trees as an extraction solvent, and a product using the same.

Recently, various pests that are harmful to humans due to climate change inhabit them in spring, summer, autumn and winter. For example, in the case of mosquitoes, the sound or vampire activity causes mental and psychological damages such as sleep disturbance, discomfort, and anxiety, and causes diseases such as malaria, encephalitis, dengue fever, and skin allergies. In addition, pests such as cockroaches, butterflies, insects and ticks are causing much damage to humans, similar to mosquitoes.

To date, efforts to address the damage caused by pests have been actively carried out around the world. During World War II, the troops stationed in the Pacific suffered a lot from pests, so research was focused on developing pest repellents that could not be applied to bite. In addition, tests have been actively conducted to develop pesticides involved in inhibiting neurotransmission of insects, inhibition of energy production, growth regulation, and inhibition of behavior. DEET (N, N-diethyl- m- toluamide), developed as a result of this effort, is the most widely used synthetic repellent among the repellents developed to date, and it is effective for various mosquito species. It is used throughout pests such as fleas and ticks, and is also used to protect livestock. Dichloro-diphenyl-trichloroethane (DDT) also attracts attention from around the world because it is very insecticidal, yet harmless to humans and livestock against mosquitoes that spread malaria or yellow fever, teeth that spread rash typhus, and fleas that spread epidemics. Synthesized.

However, most of the pest repellents and pesticides currently on the market are chemically synthesized products and cause many side effects such as environmental pollution and skin trouble. For example, DEET has an unpleasant odor, irritates the skin, causes problems such as damaging glasses or watch bands by reacting with some plastics or synthetic rubbers, and is limited in use due to fast and strong skin penetration. Follow. DDT does not decompose well when sprayed in nature, and excretion occurs very slowly when ingested in living organisms, and its use has been banned due to its great effect on ecosystem and human health, such as accumulation in living organisms.

Therefore, researches to discover natural materials for maintaining a comfortable well-being life without harming the environment away from the conventional artificial synthetic pest repellents and insecticides have been conducted mainly on plants.

Plants are plants that produce useful substances such as alkaloids, flavons, terpenes and tannins through various metabolic processes. On the other hand, there are more than 550,000 kinds of plants on the earth, but only about 1% of the plants are used by humans, and the discovery of useful materials using plants is becoming a new field of research.

Plant-derived materials can be used as raw materials for pharmaceuticals, pesticides, dyes and spices, and when used in combination with appropriate plant extracts, they can be environmentally friendly and repellent or pesticidal against various pests. Until now, researches to prepare pest repellents and insecticides using plant extracts have been conducted under such recognition, but there are not many cases of discovering substances having effects over synthetic repellents and insecticides. Therefore, by developing pesticides, soaps, detergents, shampoos, quasi-drugs, etc. containing plant extracts, there is a need to develop products that are harmless to humans and deadly to various pests.

Accordingly, the present inventors have tried to find a substance that does not harm humans while having insecticidal and repelling action of various pests among plant extracts, and as a result of extracting mugwort, moth, vinegar and mulberry as extract solvents, It was confirmed that there is a more lethal effect on the pest than the material extracted from the plant and came to complete the present invention.

It is an object of the present invention to provide a mixed extract having insecticidal and repelling action of pests.

Another object of the present invention to provide a product having a pesticidal and repellent action of the pest using the mixed extract.

In order to achieve the above object, the present invention provides a mixed extract having insecticidal and repellent action of pests extracted from mugwort, moth, mountain and mulberry with the extraction solvent.

The present invention also provides a product, such as cosmetics, detergents and fragrances, including a mixed extract having the pesticidal and repelling action of the pest.

The mixed extracts of the mugwort, moth, sancho and multi-bead tree of the present invention can be used as a pesticide because it has an excellent insecticidal effect on pests, especially mosquito larvae. The extract of the present invention is excellent in insecticidal effect than using only one of the plant extracts of mugwort, nirvana, vinegar or multi-bead tree. In addition, the extract of the present invention may contribute to the provision of preventive products such as fragrances, soaps, detergents and cosmetics by exhibiting excellent insecticidal to repellent effect against pests without abnormalities in the human body.

Hereinafter, the present invention will be described in detail.

According to one aspect of the present invention, there is provided a mixed extract having the insecticidal and repelling action of the pests extracted from the mugwort, nibber, Sancho and mulberry tree as an extraction solvent.

According to another aspect of the present invention, a method of preparing a mixed extract having a pesticidal and pest repellent action of the pest comprises the steps of mixing the mugwort, moth, mountain and mulberry; Injecting an extraction solvent into the mixture; Extracting and filtering the mixture into which the extraction solvent is added; And concentrating the filtered extract under reduced pressure.

Natural extracts of the present invention are extracts of the extracts of mugwort, nirvana, sancho and mulberry tree, the composition ratio of mugwort, nirvana, sancho and mulberry tree can be used according to the purpose of the extract. According to a preferred embodiment of the present invention, the dried mugwort, bitter gourd, sancho and mulberry tree are mixed in the order of 1: 0.5-5: 1.5-3: 0.5-5 by weight, more preferably 1: 1.5 -5: 0.5-2: 1.5-4, and most preferably 1: 2.5-3.5: 1.5-2: 1.5-3.

According to a preferred embodiment, the extraction solvent used in the present invention is purified water, methanol, ethanol, propanol, butanol, glycerol, propylene glycol, 1,3-butylene glycol, normal-hexane or chloroform, more preferably methanol, One or more solvents selected from ethanol, propanol or normal-hexane are not limited thereto, and the extraction solvent may be selected and used according to the purpose of the extract.

For example, when the present invention is used as an insecticide and a repellent, it is possible to increase the extraction content of the pesticide component by using a mixed solvent of alcohol and normal-heptane, and to select purified water when used as an antibacterial or fruit preservative. It is desirable to dissolve easily in water.

At this time, the extraction solvent in the mixture of mugwort, moth, sancho and mulberry tree is 1: 3-100 weight ratio, more preferably 1: 3-50 weight ratio. If the extraction solvent is less than 3 weight ratio, the extract is not easy to extract, if more than 100 weight ratio excess energy is consumed during concentration.

Extraction and filtration may be repeated depending on the intended use.

Extracts concentrated under reduced pressure can be used directly or diluted in water, or mixed with other active ingredients or excipients, dried and powdered, mixed with excipients, synthetic fungicides or insecticides, and processed. It may be.

The extract of the present invention is useful for insect pests including mosquitoes, flies, mites, teeth or cockroaches, and preferably has excellent pesticidal activity against larvae of mosquitoes. For example, Culex pipiens pallens, Culex tritaeniorhynchus, Culex vishnui, Culex gelidus, Culex gelidus It has excellent insecticidal activity against mosquito larvae selected from the group consisting of Culex fuscocephala, Aedes aegyti and Anopheles stephensi.

When the natural extract of the present invention is used as a pesticide composition such as pesticide, the content of the active ingredient may be appropriately adjusted according to the formulation of the pesticide and the spraying method, but 0.01 to 40% by weight is preferable. If the content of the active ingredient is less than 0.01% by weight, the pesticidal effect of the pests is insignificant, and if more than 40% by weight, sufficient insecticidal effect can be obtained, and therefore, no excess amount is required.

The mixed extract of the present invention may have a repellent effect of the pest in addition to the pesticidal effect of the pest, which can be usefully used in daily life.

Therefore, according to one aspect of the present invention, there is provided a product such as cosmetics, detergents and fragrances having insecticidal and repelling action of pests including a mixed extract of mugwort, nirvana, wild pepper and mulberry.

Cosmetics containing the mixed extract of the present invention include, but are not limited to, substances such as skins, lotions, creams, essences, nutrients, lotions and packs. In addition, the detergents include, but are not limited to, soaps, shampoos, rinses, cleansing, whole body cleansers, treatments, and cosmetic products. The fragrance of the present invention refers to a substance having a repelling effect of the pest by moving the fine particles of the extract to the pest through the air regardless of the name.

In the preparation of the product of the present invention, other active ingredients or excipients are added in addition to the mixed extract of the present invention, depending on the purpose of use. Excipients to be used may be those known in the art, such as liquid or solid carriers (diluents), electrodeposition agents, emulsifiers, humectants, dispersants, pressure-sensitive adhesives, disintegrants and the like.

Examples of liquid carriers include aromatic hydrocarbons such as toluene, xylene, alcohols such as butanol, octanol, glycols, ketones such as acetone, amides such as dimethylformamide, sulfoxides such as dimethylsulfoxide, methylnaphthylene, cyclo Petroleum fractions, such as hexanone, animal oil, vegetable oil, fatty acid, fatty acid ester, kerosene, light oil, or water, are mentioned.

Examples of the solid carrier include clay, kaolin, talc, diatomaceous earth, silica, calcium carbonate, montmillonite, bentonite, feldspar, quartz, alumina, sawdust and the like. Examples of electrodeposition agents include polyoxyethylene nonyl penny ether, polyoxyethylene lauryl ether, and the like, and examples of emulsifiers or dispersants include anionic surfactants, cationic surfactants, nonionic surfactants, amphoteric surfactants, and the like. .

For example, triton series, such as higher alcohol sodium sulfate, stearyl trimethylammonium chloride, polyoxyethylene alkyl phenyl ether, lauryl betaine, and Triton-X-100, etc. are mentioned. Examples of the moisturizing agent may include polyoxyethylene nonyl phenyl ether dialkyl sulfosuccinate, and the like, and examples of the pressure-sensitive adhesive may include capoxymethyl cellulose and polyvinyl alcohol, and the disintegrant may include sodium lignin sulfonate, Sodium lauryl sulfate.

For example, when preparing a cosmetic soap, in addition to the mixed extract, it may further contain soaps and surfactants commonly used in cosmetic soaps, and other conventional antioxidants, humectants, blotting agents, inorganic salts, perfumes, pigments, dyes, and the like. have. In addition, the cosmetic soap composition may be used in the form of an ointment or a liquid, in addition to the solid soap.

The mixed extract of the present invention may be added in an appropriate amount for the product, depending on the purpose of use. For example, when preparing a cosmetic soap, it is preferable to add a mixed extract in an amount of 2 to 20% by weight, more preferably 4 to 10% by weight, based on the product. If the mixed extract is less than 2% by weight, the pest repelling effect is insignificant, and if it exceeds 20% by weight, a sufficient pest repelling effect can be obtained, so no need to use any excess.

Products containing the natural extract of the present invention has a friendly effect on the human body and skin, while giving off aromas to avoid access to pests such as mosquitoes and insects.

Hereinafter, the present invention will be described in more detail with reference to Examples. It should be understood, however, that these examples are for illustrative purposes only and are not to be construed as limiting the scope of the present invention.

Extraction of Mugwort, Mugwort, Sancho and Mulberry Trees

Collection of natural plants

Artemisia annua , I xeris dentata Nakaki , Zanthoxylum popetitum APDG , and Melia azedarach L. , which are found in the central and northern regions of Korea, in southern Korea After being collected and collected in the air, it was dried for 10 days, avoiding direct sunlight, and made into a dried material of the above material was used as the material of the test material. The list of plants used in the present invention is shown in Table 1.

 TABLE 1

Extraction of Natural Extracts

 50g, 150g, 90g and 110g of the dried raw materials, mugwort, cilantro, sancho and mulberry, respectively, were mixed, and a total of 1000 g was added by adding 600 g of 80% ethanol. The mixture was first extracted at 35 ° C. for 12 hours and distilled under reduced pressure using an EYELA A-3s vacuum concentrator (EYELA Tokyo Rikaikai). 450 g of 50% ethanol was added as much as the amount of ethanol evaporated, followed by secondary extraction at 35 ° C. for 8 hours. After distillation under reduced pressure in the same manner as the first extraction, ethanol was evaporated after extracting 3 times with 220 g of 20% ethanol, equivalent to the amount of ethanol, at 35 ° C. for 4 hours. Filtered material No. Concentrated using 3 (Wattman). This process is shown in FIG.

Names of substances extracted from Mugwort ( A rtemisia annua ), Mulberry ( M elia azedarach L. ), Mugwort ( I xeris dentata Nakaki ) and Sancho ( Z anthoxylum popetitum APDG ) are named AMlZ after the beginning of the scientific name of raw materials. Named it.

Validation of natural extracts

Physical properties of the extract (AMIZ) using the raw material of the AMIZ extract was analyzed by HPLC (JASCO model LC900 series) and the results are shown in Table 2 and FIG. Analysis of mixed natural extracts used acetonitrile (CH3CN) / water (50:50; v / v) as the first mobile phase and 25 to acetonitrile (CH3CN) / water (80:20; v / v) as the final mobile phase. Solvent gradient was set for 5 minutes to elute the solvent every 5 minutes. The dose of AMIZ mixture was 10 μl and was detected at an optimal wavelength of 230 nm using a UV detector. As can be seen in Table 2 and Figure 2, the HPLC analysis result shows that the elution order is chlorogenic acid (tR = 4.77 min), cinerine II (tR = 10.98 min), pyrethrin II (tR = 11.33 min), and cinerin I ( tR = 17.10 min), pyrethrin I (tR = 17.40 min), and tusendinine (tR = 21.08) min). The AMIZ extract of the present invention was confirmed to include chlorogenic acid, cinerine I, II, pyrethrin I, II and tusendinin by HPLC, but due to the nature of the extract, a substance having a pesticidal or repelling effect of a pest It is not limited to these materials.

 <Table 2>

<Confirmation of pesticidal activity of natural extracts>

Preparation of Insecticidal Samples

In order to test the insecticidal activity with the extracted AMIZ solution, a total of 10 4th instar Larva, which is a mosquito larva stage of the dipetra, and 10 pupa, which are the stages of the adult stage, were tested in eight test tubes including a blank ( each test tube). Thereafter, 10 ml of each test tube was equally adjusted with purified water, and the amount of AMIZ extracts shown in Table 3 was added to confirm the number of larvae that were killed by time. Experimental conditions were 28 ℃ -30 ℃, the room temperature conditions for larvae to live, and the average value of the number of larvae killed after repeated experiments was measured.

<Table 3>

Confirming the pesticidality of AMIZ

The process of killing the larvae for 15 minutes, 30 minutes, 60 minutes, 120 minutes and 360 minutes using the AMIZ extract is shown in Table 4. In test tube 1, no larvae were killed because there was no AMIZ extract. As the concentration of AMIZ extract increased from 1.0ml to 4.0ml, the number of larvae killed increased significantly. From these results, it can be seen that as the AMIZ concentration increases, the rate of death increases.

 TABLE 4

Comparison of Insecticidal Effects of AMIZ Extracts with Individual Extracts of Mugwort, Mugwort, Sancho and Mulberry Trees

The insecticidal effect of the extracts of the individual plants, but not the mixed extracts of mugwort, moth, mountain and mulberry, was compared with the insecticidal effect of the AMIZ extract.

The process of preparing the extract of each individual plant was the same as the process of preparing the AMIZ extract, and experimented with each of the 4th instar Larva and 50 adults of Pupa. Insecticidal effect experiments were conducted on 4 larvae and pupa with the same concentration of extract from individual plants as 4.0 ml of AMIZ was added to 10 ml of purified water. , The pesticidal effect with the AMIZ of the present invention was compared. The results are shown in Table 5.

 <Table 5>

As can be seen from the table, the insecticidal effect of the four plant larvae and pupa in the case of the individual plant extracts of mugwort, moth, mountain and mulberry tree, but the insecticidal effect when compared to the AMIZ mixed extract of the present invention under the same conditions It can be seen that is low. This indicates that the AMIZ mixed extract of the present invention has a superior pesticidal effect due to the mixing.

<Verging of pests of natural extract products>

Preparation of Cosmetic Soap with AMIZ Substance

 The AMIZ extract of the present invention was added to a fat of 70 ° C.-80 ° C. which caused the saponification reaction, and then mixed with Jojoba oil and lavender scent having high unsaturated fatty acid. The mixture was cooled to 50 ° C.-60 ° C. and then aged in a flask. The mixing ratios of this experimental example are shown in Table 6.

<Table 6>

Preparation of Beauty Soap without AMIZ Substance

A cosmetic soap was prepared by treating the cosmetic soap without adding the AMIZ material in the same manner as in the experimental example. The mixing ratio of this comparative example is shown in Table 7. As the amount of the extract of AMIZ was not added, the fragrance which did not affect the repellent activity of mosquitoes was added and compared with the Example.

<Table 7>

Skin condition and repellent activity of cosmetic soap

The skin condition and the degree of mosquito evasion were confirmed with the cosmetic soaps prepared in the experimental and comparative examples, respectively. Skin condition was measured after 1 hour and divided into 13 stages, and the highest point was 13 points. The mosquito repellent activity of moisturizing soap was counted by 60 Egyptian female mosquitoes. The test was carried out on 30 adult men and women and the results are shown in Table 8.

<Table 8>

As shown in Table 8, when comparing the cosmetic soap with the addition of AMIZ up to Experimental Examples 1 to 4 and the cosmetic soap without the addition of AMIZ up to Comparative Examples 1 to 6, even if the AMIZ material is added, It did not change. In addition, the higher the concentration of the added AMIZ extract was found to be less mosquito bites.

These results indicate that the AMIZ extract of the present invention, when used in cosmetic soaps, detergents and cleansing agents, exhibits a repelling effect from pests such as mosquitoes without harming humans.

1 is a view showing a process for identifying the extraction method of AMIZ and the components contained in the extract.

FIG. 2 is a chromatogram showing the results of detection at 230 nm, which is an optimum wavelength, after AMIZ was separated by HPLC.

Claims (7)

Mugwort, moth, moss, and mulberry tree are mixed in a ratio of 1: 2.5-3.5: 1.5-2: 1.5-3 by weight, ethanol solvent is added at 35 ° C, followed by primary extraction for 12 hours and for 8 hours. After the second extraction, the method of producing a mixed extract having a pesticidal and repelling action of the pest comprising a third extraction for 4 hours. The method of claim 1, The pest is a mixed extract manufacturing method having a pesticidal and repelling action of pests, including mosquitoes, flies, mites, teeth or cockroaches. A mixed extract prepared according to the method of claim 1, wherein the mixed extract is prepared. Cosmetics comprising the mixed extract of claim 3. Perfume comprising the mixed extract of claim 3. A detergent comprising the mixed extract of claim 3. delete

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