KR100852204B1 - An antifungal composition comprising an extract of corallina sp - Google Patents

Abstract

A composition comprising the extract of Corallina sp. is provided to exhibit antifungal activity against Malassezia sp. which is a fungus causing dandruff or atopic dermatitis without side effects, so that the composition is useful for preventing or treating dandruff or atopic dermatitis. A composition exhibiting antifungal activity against Malassezia sp. selected from Malassezia furfur, Malassezia restricta, Malassezia obtusa, Malassezia globosa, Malassezia slooffiae, Malassezia sympodialis and Malassezia pachydermatis comprises the extract of Corallina sp. including Corallina pilulifera and Corallina officinalis, which is prepared by extracting Corallina sp. with an extraction solvent selected from ether, chloroform, benzene, hexane, dichloromethane and a mixture thereof.

Description

Antifungal composition comprising coral extract {AN ANTIFUNGAL COMPOSITION COMPRISING AN EXTRACT OF CORALLINA SP.}

The present invention relates to a composition having antifungal activity against Malassezia sp. Fungi.

Dermatitis is an inflammation of the skin and is usually used in the same sense as eczema. It is classified into atopic dermatitis, contact dermatitis and seborrhoeic dermatitis. The atopic dermatitis is an eczema-like skin lesion that occurs in atopic dermatitis, also referred to as endogenous eczema or Benie yangjin. The contact dermatitis refers to inflammation of the skin caused by contact with an external substance. It is so-called skin rash that is characterized by itching. In addition, the seborrhoeic dermatitis refers to dermatitis, which occurs well in areas with high secretion of sebum, such as the head, forehead or armpit, and is also called seborrheic eczema. It is a dermatitis mainly caused by erythema and slenderness, or dandruff, and the lightest type is dandruff or scaly tetter.

The dandruff occurs when a problem occurs in the general cycle of skin cells, also known as dry seborrhea, a slight form of seborrheic dermatitis in the scalp, mainly after puberty in which sebaceous glands are actively active, but immediately after birth. Can occur in both sexes.

Recently, it is known that the causative agent of dandruff is a fungus, that is, a yeast bacterium. Especially, it is caused by Malassezia sp. It is reported that if the Malassezia fungi overgrows due to environmental factors such as food and food, dandruff symptoms such as sewage and itching appear, and when overgrowth is severe, seborrheic dermatitis occurs (Bergbrant IM Seborrhoeic dermatitis and Pityrosporum yeasts.Current Topics in Medical Mycology. 6: 95-112 (1995)). In addition, when a lot of dandruff occurs, the scalp is excessively rubbed due to itching, thereby causing a wound or, in some cases, causing inflammation and causing hair loss.

Recent studies have shown that Malassezia furfur , previously known as a causative agent of dandruff, is associated with seborrheic dermatitis, which occurs mainly in the trunk, and is rarely present in the scalp. Other species, Malassezia restrica and Malassezia globosa Has been identified as a major causative agent of dandruff (Christina M. Gemmer, Thomas L. Dawson Jr, et al., Journal of Clinical Microbiology , Sept Vol. 40, No. 9, p3350-3357 (2002)). After the above findings, recent studies have confirmed that Malassezia restrica and Malassezia globosa are the causative agents of dandruff and Malassezia furfur is related to seborrheic dermatitis of the torso skin and not scalp.

According to the results of the above study, in order to treat, prevent or improve dandruff and itching, the composition contains a component capable of inhibiting the growth of Malassezia sp. As an essential requirement, commercially available compositions such as ketoconazole, zinc pyrithion, itraconazole, or pyroctone olamine have been mainly used. In addition, tar, sulfur, salicylic acid, and the like have been used to relieve itching and tearing, and selenium sulfide, etc., has been used to suppress hyperproliferation of epidermal cells. However, while the antifungal agents that have been used for the treatment of dandruff have a good effect on the treatment of dandruff, most of them are questions about human safety because they are artificially synthesized materials.

For example, long-term use of ketoconazole, an active ingredient of an anti-dandruff treatment such as nizoral, which is commercially available in Korea, poses a problem that may cause adverse effects on the endocrine system (Schuermeyer T, Nieschlag E. 1982. Ketoconazole induced drop in serum and saliva testosterone.Lancet 1098p; Janssen PAJ, Symoens JE. 1983. American Journal of Medicine 74: 80-85). In addition, zinc pyrithione exhibits excellent effects as an antifungal agent for inhibiting scalp hyperkeratosis, but is insoluble in water and has a high specific gravity, which makes it difficult to spray uniformly in the composition. Gene A. Hyde and Mark M. Auerbach, Formulation techniques for zinc pyrithione antidandruff shampoos.Cosmetics and Toiletries, 94, 57, April (1979); Fred H. Snyder et al , Safety evaluation of Zinc 2-Pyridiethiol 1-Oxide in Shampoo Formulation, Toxicology and Applied Pharmacology, vol. 7, p. 425-437 (1965); Marks, R. The effects of a shampoo containing zinc pyrithione on the control of dandruff.Br J. Dermatol. 112. 415 (1985). In particular, zinc pyrithione causes skin dermatitis (Nielsen, NH, Allergic contact dermatitis caused by zinepyrithione associated with pustular psoriasis. Am J. Contact Dermat. 8, 170. 1997). It is reported that it is difficult to apply it to hair cosmetics at high concentration.

In addition, itraconazole, which has been studied to replace ketoconazole, is highly effective against many pathogenic fungi related to humans and has a high affinity for keratinous tissues (Van Cutsem J, Van Gerven F, Zaman R, Heeres J, Hanssen PAJ.Pharmacological and preclinical results with a new oral and topical broad spectrum antifungal R 51211. Proceedings of the 13 ^th International Congress of Chemotherapy, Vienna, Part 40: 1-11 (1983)), Ketoro As it is not transported through sweat glands like Nazol, it has a disadvantage of being slightly slower when taken with oral medication. It is reported that at least 1,000 mg of prescription is required daily to prevent satisfactory treatment and recurrence (Grant SM, Clissold). SP. Drugs 37: 310-344 (1989). In addition, pyroctonolamine and salicylic acid, which are mainly used as sterilization and keratin solubilizers, were studied to make up for the defects of zinc pyrithione.

In addition, the majority of patients are prescribed several kinds of drugs at the same time, but the administration of the combination may cause problems that may affect the toxicity and immune system of the liver cells and increase the efficiency or adverse effects of one drug. Svejgaard E. Side effects and interactions of oral antifungals.Journal of the European Academy of Dermatology and Venereology 5, page S11 (1995); Bickers DR. Journal of the American Academy of Dermatology 31: S87-S90 (1994), headache Or symptoms such as nausea (Georgiou S, Monastirli A, Pasmatzi E, Zografakis C, Tsambaos D. Oral itraconazole therapy for pityriasis versicolor.Journal of the European Academy of Dermatology and Venereology 8: 203-207 (1997)) .

Dandruff is not a shortened or serious illness, but it is a psychological stress for most people. Methods and ingredients to prevent or treat such dandruff are continuously being studied, but as mentioned above, there are still few studies on ingredients that are ecologically friendly, harmless to the environment and human, and which can bring about a reduction in the number of dandruff bacteria. to be.

 Therefore, unlike the existing antifungal substances, while having excellent antimicrobial activity against Malassezia sp. Fungi, the causative agent of dandruff, there is no problem of resistance or stability without long-term intake or use. In addition, there is a growing demand for the development of an improved, preventive and therapeutic composition for natural dandruff derived from natural substances that do not cause any side effects. In particular, the three sides are the sea, considering the geographical environment of Korea, which is rich in marine resources, there is an increasing demand to develop a composition capable of preventing or treating dandruff from marine natural substances including marine animals and plants.

The present invention is to improve the problems of the prior art as described above, and antimicrobial composition against Malassezia sp. Fungi, which is a causative agent of dandruff and / or seborrheic dermatitis, including seaweed extract as an active ingredient An object of the present invention is to provide a composition for preventing or treating at least one selected from the group consisting of dandruff and seborrheic dermatitis.

In order to achieve the above object, the present invention provides an antimicrobial composition against Malassezia sp. Fungi comprising Corallina sp. Seaweed extract as an active ingredient.

In addition, in order to achieve the above object, the present invention provides a composition for the prevention or treatment of dandruff and / or seborrheic dermatitis comprising the antimicrobial composition as an active ingredient.

In addition, in order to achieve the above object, the present invention provides a cleaning composition for preventing or improving dandruff and / or seborrheic dermatitis comprising the antimicrobial composition as an active ingredient. The detergent composition may be applied to the hair and / or scalp or to the skin.

In addition, to achieve the above object, the present invention provides a cosmetic composition for the prevention or improvement of dandruff and / or seborrheic dermatitis comprising the antimicrobial composition as an active ingredient. The cosmetic composition may be applied to the hair and / or scalp or to the skin.

In addition, in order to achieve the above object, the present invention provides a food composition for the prevention or improvement of dandruff and / or seborrheic dermatitis comprising the antimicrobial composition as an active ingredient. Examples of the food composition of the present invention include food, food additives, beverages or beverage additives.

Hereinafter, the present invention will be described in more detail.

In the present invention, coralline in (Corallina sp.) Seaweeds beonmu throughout primarily a tropical or subtropical with seaweed belonging to red algae jinu Ari neck coralline and (Corallinaceae) during algae and are the main types that make up so-called reef. Of the genus corals horse the coral, and a marine algae that is, yujeol coral algae belonging to malahgwa (Corallinoideae) is kind of a word that does not harden with calcified body coral, one of the limestone algae is most commonly grown in the leading edge of the Republic of Korea, It lives mainly on rocks of intertidal zone. The coral algae in the coral may preferably be any one or more selected from the group consisting of Corallina pilulifera and Corallina officinalis , and more preferably may be small bead coral.

In the present invention, Corallina pilulifera is a seahorse belonging to the red algae Coral family, and is a representative species belonging to the articulated coralline algae. The small bead coral foot is small, the height is 3 to 4cm, dense and flock together. It is mainly distributed in Japan and Korea, and can be found generally in Korea's previous years.

The extraction solvent of the present invention may be any one selected from the group consisting of nonpolar solvents of ether, chloroform, benzene, hexane or dichloromethane and mixed solvents thereof, preferably consisting of ether, chloroform, benzene, hexane and dichloromethane. It may be any one selected from the group, more preferably ether. In particular, it was confirmed that the antifungal activity against Malassezia fungi, the causative agent of dandruff, was found in the case of small bead esoteric ether extract. On the other hand, the antifungal activity against Malassezia fungi was not observed in extracts with polar solvents such as water or methanol (see Table 1).

Coral seaweed of the present invention may be prepared according to the conventional seaweed extract preparation method, specifically may be cold extraction, hot extraction or heat extraction, etc., using a conventional extraction device, ultrasonic grinding extractor or fractionator Can be.

In addition, the extract extracted with the solvent may be further subjected to the fractionation process with a solvent selected from the group consisting of a non-polar solvent of ether, chloroform, benzene, hexane, methylene chloride or dichloromethane and a mixed solvent thereof. The fractionation process may be performed at 4 ℃ to 120 ℃, but is not limited thereto. In addition, at least one solvent may be used in the fractionation.

The prepared extract or the fraction obtained by performing the fractionation process can be filtered or concentrated or dried to remove the solvent, it can be carried out both filtration, concentration and drying. Specifically, the filtration may be performed using a filter paper or a reduced pressure filter, the concentration may be concentrated under reduced pressure using a reduced pressure concentrator, for example, a rotary evaporator, and the drying may be performed by, for example, a lyophilization method.

In one embodiment of the present invention, the seaweed extract of the genus Coral can be obtained by the process shown in FIG. More specifically, after removing the salt and impurities of the seaweed in the coral, for example small bead corals, and dried to prepare a dry sample, and adding ether to the dried sample for 4 hours to 36 hours, preferably 12 hours To extract crude at room temperature for 24 hours to obtain a crude extract, the crude extract is concentrated under reduced pressure. At this time, the salt and impurities of the small bead coral may be removed by rinsing with running water, and the dried sample may be prepared by drying the small bead coral after removing the salt and impurities. In addition, the obtained small bead Coral extract can be stored in a deep freezer until use.

In addition, the coral algae extract in the coral may be obtained by completely removing the water through the concentration and lyophilization of the obtained extract, the coral algae extract in the complete removal of the water is used in powder form or the powder ether Or it can melt | dissolve in a normal nonpolar solvent and can be used.

The present invention relates to an antimicrobial composition against Malassezia fungi comprising an extract of the genus Coral seaweed as an active ingredient. The coral algae extract may be a crude extract obtained by the extraction solvent or a fraction obtained by the fractional solvent.

In the present invention, the antimicrobial composition may have the same meaning as an antibiotic which is a generic term for an antimicrobial agent, and may have the same meaning as an antifungal agent, a fungicide, a preservative, a preservative or an antifungal agent, and preferably, such as seborrheic dermatitis or dandruff. By pathogenic microorganisms, including Malassezia sp. Fungi, which cause skin diseases, it means substances that can inhibit or inhibit the development and life functions of fungi.

In the present invention, the genus Malassezia means all fungi belonging to the genus Malassezia , for example, the genus Malassezia is Malassezia restricta , Malassezia globosa , Maleesia Puffer ( Malassezia furfur ), Malassezia obtusa , Malassezia slooffiae , Malassezia sympodialis and Malassezia pachydermatis ( Malassezia pachydermatis ) It may be any one or more selected from the group, and more preferably may be any one or more selected from the group consisting of Malaysian restricta, Malesesia globosa, Malesesia puffer and Malesesia obtusa.

The antimicrobial composition may include 0.001 to 99.99% by weight, preferably 0.1 to 99% by weight of the seaweed extract in the coral, based on the total weight of the composition, depending on the method and purpose of use of the antimicrobial composition. The content can be adjusted appropriately.

The antimicrobial composition of the present invention may include sugars, proteins, lipids, vitamins and minerals in addition to the seaweed extract of the coral. The sugars, proteins, lipids, vitamins or minerals may be appropriately selected according to the purpose and use thereof. For example, the sugars may be honey, dextrin, sucrose, palatinose, glucose, fructose, starch syrup, sugar alcohol. , Sorbitol, xylitol, maltitol, the protein may be milk-derived protein such as casein, whey protein, soy protein, trypsin of these proteins, animal derived enzymes such as pepsin and neutrase ), Hydrolyzate by alkilase, the lipid may be monohydric saturated fatty acid, sunflower oil containing polyunsaturated fatty acid, rapeseed oil, olive oil, safflower oil, corn oil , Oils such as soybean oil, palm oil, palm oil, and other plant-derived oils, middle-chain fatty acids, EPA, DHA, soybean-derived phospholipids, milk-derived phospholipids, Lal may be potassium phosphate, potassium carbonate, potassium chloride, sodium chloride, calcium lactate, calcium gluconate, calcium pantothenate, calcium casein, magnesium chloride, ferrous sulfate, sodium hydrogen carbonate, but is not particularly limited by the examples. .

For any of the above saccharides, proteins, lipids, vitamins and minerals, as long as the antimicrobial properties of the composition are maintained, not limited to the above embodiments and may include other ingredients, each content is not limited as long as the antimicrobial properties are maintained Preferably, it may be 0.1 to 15% by weight, preferably 0.5 to 10% by weight relative to the total composition.

In addition, the antimicrobial composition maintains the effects of the present invention, and does not cause side effects due to the added ingredients, in addition to the active ingredient of various cosmetic compositions for the purpose of sterilizing Malassezia fungi and dermatology Additives, preferably in the group consisting of ketoconazole, zinc pyrithion, itraconazole, pyroctone olamine, selenium sulfide, tar, sulfur and salicylic acid, which are common anti-dandruff components It may further comprise any one selected, and such additional ingredients or additives may be provided so as not to interfere with other compositions of the composition.

The antimicrobial composition of the present invention can be used for a variety of purposes and uses that require antimicrobial activity, specifically can be used for the purpose of inhibiting the growth of pathogenic microorganisms, for example pathogenic fungi, dandruff or dermatitis caused by pathogenic fungi Preferably, it can be used for the purpose of disinfection of the genus Malassezia or for the treatment, prevention or improvement of dandruff or seborrheic dermatitis caused by the genus Malassezia.

More specifically, the antimicrobial composition of the present invention has antimicrobial activity against the male causative bacteria that cause causal bacteria among the genus Malassezia, which is the causative agent of dandruff, thereby treating, improving or treating dandruff. Since it has been found to have a remarkably excellent effect on prevention, it can be applied to a composition for preventing or treating dandruff or a cleaning composition or a cosmetic composition which can be applied to at least one selected from the group consisting of hair and scalp. In addition, since it exhibits antimicrobial activity to male causal puffer and male obesity, which are the causative agents of seborrheic dermatitis mainly in the trunk, to prevent or treat seborrheic dermatitis or to improve or prevent seborrheic dermatitis. It can be applied to a cleaning composition or a cosmetic composition that can be applied to the skin.

The present invention also provides a composition for preventing or treating at least one, preferably dandruff, selected from the group consisting of dandruff and seborrheic dermatitis comprising the antimicrobial composition as an active ingredient. Coral algae extract of the present invention is at least one selected from the group consisting of Malassezia fungi, more specifically maleesia restrista and maleesia globosa, the causative agent of dandruff, maleses that are the causative agents of seborrheic dermatitis. Since it has antifungal activity against one or more fungi selected from the group consisting of Zia puffer and maleseviia orbusa, the antimicrobial composition is one or more selected from the group consisting of dandruff and seborrheic dermatitis. Has a therapeutic effect.

In the present invention, dandruff refers to the skin of the head, ie, the stratum corneum cells of the scalp, simply falling off without inflammation, and appearing in dry or damp or greasy condition. Many cases of dandruff are referred to as dandruff, medically referred to as dry seborrhea. When the dry seborrhea is worsened, it may be a dermatitis seborrheica, and the seborrheic dermatitis may be accompanied by deprivation and itching of a large amount of dry scales. In addition, alopecia which occurs when the dandruff caused by dry seborrhea is chronically severe is called alopecia seborrheica. In case of the dandruff or seborrheic dermatitis, the main causative agent is reported as the genus Malassezia fungi. Therefore, in order to treat the dandruff or seborrheic dermatitis, it is possible to prescribe a substance having antibacterial activity against Malassezia fungi, the causative bacterium.

At least one preventive or therapeutic composition selected from the group consisting of dandruff and seborrheic dermatitis of the present invention can be applied directly to animals including humans. The animal is a biological group corresponding to plants, and mainly ingests organic matter as nutrients, and means that digestion, excretion, and respiratory organs are differentiated, preferably mammals, and more preferably humans.

The at least one preventive or therapeutic composition selected from the group consisting of dandruff and seborrheic dermatitis may include the antimicrobial composition alone as an active ingredient, and additional ingredients, ie, pharmaceuticals, depending on the formulation, method of use, and purpose of use. It may further comprise an acceptable or nutritionally acceptable carrier, excipient, diluent or accessory.

More specifically, at least one composition for prevention or treatment selected from the group consisting of dandruff and seborrheic dermatitis, in addition to the active ingredient, nutrients, vitamins, electrolytes, flavors, coloring agents, neutralizing agents, pectic acid and salts thereof, alginic acid And salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, and the like. In addition, the carrier, excipient or diluent may be lactose, dextrose, sucrose, sorbitol, mannitol, ziitol, erythritol, maltitol, starch, acycia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline Group consisting of cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, dextrin, calcium carbonate, propylene glycol, liquid paraffin, physiological saline It may be one or more selected from, but is not limited to any conventional carriers, excipients or diluents can be used. The components may be added independently or in combination to the antimicrobial composition that is the active ingredient.

At least one prophylactic or therapeutic composition selected from the group consisting of dandruff and seborrheic dermatitis is 0.001% to 99.9% by weight of the antimicrobial composition, preferably 0.1% to 99% by weight, more preferably based on the total weight of the composition. May include 1% to 50% by weight. In addition, the content of the additional component is preferably added in the range of 0.1 to 20 parts by weight per 100 parts by weight of the antifungal composition.

In addition, at least one preventive or therapeutic composition selected from the group consisting of dandruff and seborrheic dermatitis, when formulated, conventional fillers, extenders, binders, disintegrants, surfactants, anticoagulants, lubricants, wetting agents, fragrances, emulsifiers Or may further include preservatives, and can be used orally or parenterally.

Specifically, solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations include at least one excipient such as starch, calcium carbonate, water, and the like in the antimicrobial composition. Prepared by mixing sucrose or lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include suspensions, solvents, emulsions, and syrups.In addition to the commonly used simple diluents, water and liquid paraffin, various excipients may include, for example, wetting agents, sweeteners, fragrances, and preservatives. have.

The form for parenteral administration may be parenteral, such as topical agents such as creams, ointments, dressing solutions, sprays, and other coatings. As an example of the dosage form of the topical dosage form, an antifungal composition or an antifungal composition selected from the group consisting of dandruff and seborrheic dermatitis is impregnated into a carrier such as gauze made of natural or synthetic fibers. Can be. When formulated as a parenteral preparation which is a form for parenteral administration, it is prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents and surfactants that are commonly used. Preparations for parenteral administration may include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin, glycerol or gelatin may be used.

In the case of the cream or ointment, it may be suitable for direct application to or around the affected area of one or more diseases selected from the group consisting of dandruff and seborrheic dermatitis. In the case of the spraying agent, it can be prepared by a conventional spraying method, except that it contains an antimicrobial composition as an active ingredient, and is packed in a compression container or other spraying container and sprayed on the dandruff site from time to time to prevent dandruff or seborrheic Dermatitis can be prevented or treated.

In addition, the formulation of the one or more prophylactic or therapeutic compositions selected from the group consisting of dandruff and seborrheic dermatitis of the present invention may be in a preferred form depending on the method of use, in particular the rapid, continuous or Methods known in the art can be employed and formulated to provide delayed release. Examples of specific formulations include warnings, granules, lotions, liniments, limonades, fragrances, powders, syrups, ointments, liquids, aerosols and extracts. , Elixier, ointment, liquid extract, emulsion, suspension, premises, acupuncture, eye drops, tablets, suppositories, injections, spirits, cataplasma, capsules, creams, pills, troches ), Tincture, pasta, soft or hard gelatin capsules.

Furthermore, one or more compositions for the prophylaxis or treatment selected from the group consisting of dandruff and seborrheic dermatitis of the present invention can be prepared using appropriate methods known in the art or by Remington's Pharmaceutical Science (Recent Edition), Mack Publishing Company. , Easton PA).

The dosage of the at least one prophylactic or therapeutic composition selected from the group consisting of dandruff and seborrheic dermatitis according to the present invention is appropriately determined by those skilled in the art in consideration of the method of administration, the age, sex and weight of the recipient, and the severity of the disease. Can be selected. For example, at least one preventive or therapeutic composition selected from the group consisting of dandruff and seborrheic dermatitis of the present invention is 0.0001 mg / kg to 1000 mg / kg, based on the antimicrobial composition, to be more effective 0.01 mg / kg to 100 mg / kg. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

In addition, the composition for preventing or treating at least one selected from the group consisting of dandruff and seborrheic dermatitis of the present invention may further comprise a compound or plant extract having an inhibitory activity of known dandruff or seborrheic dermatitis in addition to the antimicrobial composition. 5 parts by weight to 20 parts by weight based on 100 parts by weight of the antimicrobial composition.

The present invention also provides a cleaning composition for preventing or improving at least one selected from the group consisting of dandruff and seborrheic dermatitis comprising the antimicrobial composition as an active ingredient. The detergent composition may be applied to the hair and / or scalp or to the skin.

The present invention also provides a cosmetic composition for preventing or improving at least one selected from the group consisting of dandruff and seborrheic dermatitis comprising the antimicrobial composition as an active ingredient. The detergent composition may be applied to the hair and / or scalp or to the skin.

In the present invention, hair refers to human hair as a generic term for body hair and hair, preferably hair, and may include hair roots of hair, hair follicles, hair and scalp near hair follicles. In the present invention, the scalp refers to the skin covering the head area, usually the hair (hair) area.

The detergent composition may be applied to one or more selected from the group consisting of hair and scalp, or may be applied to the skin, and is also referred to as a body cleanser composition when applied to the skin.

The cleaning composition comprising the antimicrobial composition as an active ingredient is a substance commonly used in the preparation of the active ingredient in addition to the active ingredient, for example, a surfactant, a tax aid, a pH adjuster, a moisturizing agent such as a natural moisturizing ingredient and a natural vegetable oil, and an antioxidant. , Metal ion sequestrants, preservatives, solvents, flavoring agents and pigments may be further included. In addition, it may include additional antimicrobial substances or fungicides within the scope of maintaining the antimicrobial effect of the present invention, and does not irritate the human body.

The detergent composition is a composition used to remove oily and aqueous pollution or wastes attached to the skin surface with moderate foaming power in cold water and hot water. Recently, moisture is evaporated while supplying moisture to the skin. It provides additional functions such as moisturizing effect, skin flexibility, and sebum removal ability. In addition, recently, the skin hypoallergenicity of the cleaning composition is emphasized.

In general, inflammation of human skin is caused by bacteria in the sebum in the pores, or caused by allergic factors. When washing with the cleaning composition, the skin condition is obtained by obtaining skin sebum removal and sterilization effects depending on the ingredients contained therein. Can be improved. In addition, the role of removing the waste or sebum in the cleaning composition is mainly a surfactant, and bacterial infection prevention is performed by a fungicide or an antimicrobial agent, since the currently used fungicide or antimicrobial material is mainly a chemical synthetic material, a chemical synthetic material When used on sensitive skin or excessive use over a certain level there is a problem that can cause skin damage by contact with the skin.

Therefore, efforts to replace the fungicides or antimicrobial substances with skin hypoallergenic or harmless natural substances are continued, and in this respect, the seaweed extracts in corals, which are the antimicrobial components of the antimicrobial composition of the present invention, are edible. Natural plant has the advantage of hypoallergenic skin.

In addition, the antimicrobial composition is a strain causing Malassezia fungi, in particular male puffer, which causes the seborrheic dermatitis mainly on the body, and maleesia restricta, which is the cause of dandruff and male dandruff. Since it shows excellent antimicrobial activity against and maleesia globosa, the cleaning composition of the present invention has a cleaning and moisturizing effect which is a basic function of the cleaning composition, is hypoallergenic, at least one selected from the group consisting of dandruff and seborrheic dermatitis. Remarkably excellent effects are recognized in that they can be improved or prevented.

 The surfactant may be any one selected from the group consisting of anionic surfactants, nonionic surfactants, cationic surfactants and amphoteric surfactants.

The anionic surfactant may be, for example, ammonium lauryl sulfosuccinate, ammonium lauryl sulfate, sodium cocoyl isionate, sodium lauryl isceionate, sodium lauryl sulfate, triethanolamine lauryl sulfate or sodium lauryl ether sulfate And the like. The nonionic surfactant may be, for example, polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene, polysorbate 80, hardened castor oil derivative, fatty acid diethanolamide, glyceryl stearate, or the like. The cationic surfactant may be, for example, a tertiary aliphatic amine salt, alkyltrimethylammonium chloride, or dialkal methylammonium chloride. The amphoteric surfactant may be, for example, betaine, amidebetaine type, sulfobetaine type, or steartrimonium chloride.

The pH adjusting agent is an organic acid, such as citric acid, succinic acid, tartaric acid, lactic acid, malic acid, fumaric acid, maleic acid, glycolic acid, hydrochloric acid, sulfuric acid, phosphoric acid, nitric acid, or the like, or a combination of sodium, potassium, or ammonium salts of these acids. Triethanolamine, sodium chloride, sodium hydroxide or potassium hydroxide, and the like.

The moisturizing agent is a conventional oil used for moisturizing, for example, vegetable oil or liquid paraffin, ethylene glycol, diethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, sorbitol, Collagen and the like. The moisturizing agent may be contained in an amount of 0.1 to 10 parts by weight based on the total weight of the composition, but is not limited thereto.

The preservative may be, for example, benzoic acid, paraoxybenzoic acid ester, a mixture of methylchloroisothiazolinone, phenoxyethanol, DMDM hydantoin, methyl paraben, ethyl paraben, or propyl paraben.

The solvent may be, for example, purified water, ethanol, Tween 20, cyclomethicone, mineral oil, or dimethicone, and the anti-corrosive agent and the pigment may be selected by a person having ordinary skill in the art to which the present invention belongs. The components used in the composition can be used.

The detergent composition may also be formulated as a liquid, solid soap or powder.

If the detergent composition is to be applied to at least one selected from the group consisting of hair and scalp, the detergent composition may be shampoo, rinse, shampoo and rinse blend, treatment, soap, bar or tablet suitable for cleaning skin, soap bar. Or a soap tablet or the like.

When the detergent composition of the present invention is used for shampoo and hair and scalp cleansing, it may further include a surfactant. The surfactant may be, for example, any one or more selected from the group consisting of synthetic anionic surfactants, amphoteric surfactants and nonionic surfactants, the anionic surfactants may be alkylsulfate or alkylethersulfate, The amphoteric surfactant may be alkylbetaine or alkylamidopropylbetaine, the nonionic surfactant may be alkanolamide or alkylamine oxide, and the surfactant may be used alone or in combination.

In addition, the hair and scalp cleansing composition may further include conventional ingredients for maintaining the basic properties and quality of the cleansing use, such as shampoo, the components are pearlescent supplements, preservatives, thickeners, viscosity regulators , pH adjusting agents, perfumes or dyes may be further included.

The pearl foil auxiliary agent may be ethylene glycol monostearate or ethylene glycol distearate, and the preservative may be methyl paraoxybenzoate, methylchloroisothiazolinone, methylisothiazolinone, or a mixture thereof, The agent and the viscosity adjusting agent may be sodium chloride, ammonium chloride or propylene glycol, the pH adjusting agent may be citric acid, phosphoric acid, sodium hydroxide or potassium hydroxide, the dye may be a water-soluble tar and the like. In addition, the hair and scalp cleansing composition may further include a hair conditioning agent such as polyquaternium-10, polyquaternium-7, methylpolysiloxane, dimethylconcopolyol or hydrolyzed animal protein.

The cosmetic composition may be for applying to the hair and / or the scalp or to the skin, for example may be a hair cosmetic composition.

When the cosmetic composition is a hair cosmetic composition, a hair conditioner, hair tonic, hair cream, hair lotion, hair face, hair gel, hair pack, hair massage, hair liquid, hair spray, hair mousse, aerosol mousse, and aerosol spray , Aerosol mousse, aerosol spray, perm, bleach, water dispersion, powder or oil. The composition of each formulation may contain a variety of bases and additives necessary for the formulation of the formulation, and the type and amount of these components can be easily selected by those skilled in the art within the range that can maintain the effect of the active ingredient. have.

When the formulation of the hair cosmetic composition is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silita, talc or zinc oxide may be additionally added. It may include.

When the formulation of the hair cosmetic composition is a powder or a spray, it may further include lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder, and in particular, when the formulation is a spray, additionally chlorofluoro Propellants such as hydroxycarbons, propane, butane or dimethyl ether.

When the formulation of the hair cosmetic composition is a solution or emulsion, it may include a solvent, a solubilizer or an emulsifier, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, Fatty acid esters of 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan, and the like.

When the formulation of the hair cosmetic composition is a suspension, a liquid diluent such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like may further be included.

The antimicrobial composition comprising the coral horse extract of the present invention as an active ingredient has an antimicrobial activity against the fungus Malassezia sp., Which is a causative agent of dandruff, and thus has an excellent preventive or therapeutic effect against dandruff. Unlike conventional antibiotics, it contains an extract extracted from coral algae, which is used for food, as an active ingredient, so there is no side effect, and it is economically advantageous because it uses coral algae, which is rich in algae. As the effect will be very large.

Hereinafter, preferred embodiments of the present invention are described. However, the following examples are only preferred embodiments of the present invention, and the present invention is not limited to the following examples.

Example 1 Preparation of Small Bead Coral Extract

Example 1-1 Preparation of Small Bead Coral Extract

The small beaded corals used for the test were collected from offshore of Cheongsapo, Busan in March 2006. The collected small beaded corals were removed with deposits, washed with clean fresh water, completely dried at room temperature, and then crushed using a grinding machine. It was. Extracts of the ground algae were prepared by the method as described in FIG. 1.

In more detail, as shown in FIG. 1, 300 ml ether or methanol was added to 20 g of the pulverized small bead-foam pulverized powder, and extracted at room temperature for one day to obtain a crude extract. The crude extract was filtered, the filtrate was concentrated under reduced pressure using a vacuum evaporator, sterilization was performed using a 0.22 μm filter, and the concentration of the sterilized extract was adjusted to 40 mg / ml, respectively, ether extract and Methanol extracts were prepared. Each ether extract and methanol extract obtained above was stored at -20 ° C in a deep freezer until use.

The methanol extract was filtered and 100 ml of methanol and the same amount of distilled water were added to the remaining residue, followed by extraction at room temperature for one day, followed by sterilization using a 0.22 μm filter, and the concentration of the sterilized water extract to 40 mg / ml. Adjusted to prepare a water extract. Each of the water extracts obtained was stored at -20 ° C in a deep freezer until use.

At this time, the total amount of the ether extract, methanol extract and water extract obtained from 20 g of the small bead-foam dry pulverized powder was 0.14 g, 0.73 g and 0.4 g, respectively.

Example 2: Antifungal Activity Experimental Preparation of Small Bead Coral Extract

For the crude extract of the small bead-coral equine prepared in Example 1, antifungal activity against Malassezia sp. Fungi, the causative agent of dandruff, was measured using the Agar diffusion method.

Example 2-1: Target strain and culture medium

Malassezia restricta strains (KCTC 7848) and Malassezia furfur strains (KCTC 7743), which are the Malassezia sp. Fungi strains, were distributed and used at the Korea Institute of Biotechnology and Biotechnology Center (KCTC), and the strains had the following composition: Malassezia restricta and Malassezia furfur were incubated at 34 ° C using culture medium.

Composition of culture medium: Modified Leeming and Notman agar medium (0.1% polypeptone, 2.0% glucose, 5.0% Malt extract, 2.0% desiccated ox bile, 0.2% glycerol, 1.0% Tween 40 and 2.0% agar in distilled water)

Example 2-2: Determination of Antifungal Activity Using Paper Disks

Antifungal activity against Malassezia sp. Fungi, the causative agent of dandruff, was carried out by plate medium diffusion using a paper disk.

The antifungal activity was used for paper discs containing each small bead kohlrabi extract prepared in Example 1 which is an antifungal target material.

First, each 5 mg extract of each small bead coral extract of Example 1 was dissolved in 30 μL of the same solvent as the extraction solvent, and then a paper disk (Advantec Filter Paper, Toyo Roshi Kaisha Ltd) having a diameter of 8 mm and a thickness of 1.5 mm , Japan), the solvent was completely evaporated, and then placed on a plated medium (NANA) plated with a strain of Malassezia fungi cultured in the medium of Example 2-1. The activity was tested, and the control group was tested for antimicrobial activity by performing the absorption and evaporation process using the same amount of the same solvent only. The strain was cultured Malassezia restricta and Malassezia furfur in a 34 ℃ condition using a culture medium having the following composition, and incubated for 48 hours at the above conditions, the size of the circular growth ring formed around the disk (in mm) was measured . The experiment was repeated three times independently, to obtain the average value of the three experiments are shown in Table 1 below.

As a result of measuring the antifungal activity, as shown in Table 1 and FIG. 2 and FIG. 3, the ether extract of small bead coral was found to have excellent antifungal activity against Malassezia restricta and Malassezia furfur . As shown in Figure 1 and Figures 4 to 7, the water extract or methanol extract of small bead coral was found to have no antifungal activity.

The numerical value shown in the following table is the value of the diameter of the inhibitor ring excluding the size of the diameter of 8 mm ((measured value-8 mm) / 2).

More specifically, in the case of the ether extract prepared in Example 1, the paper disk, compared to the control group without using the extract, Malassezia Antimicrobial activity was observed from 3 mg for restricta and from 1 mg for Malassezia furfur. The antifungal activity was also improved in proportion to the concentration of extract used for each fungus. On the other hand, methanol extracts and water extracts were found to have no antifungal activity at all when the extracts 1mg, 3mg and 5mg were used.

Taken together, the results of the antifungal activity against Malassezia sp. Fungi, the causative agent of dandruff, were determined using the Agar diffusion method. determined that no antifungal activity for the extract with, a non-polar solvent is an ether extract of the case causative organism of dry count subgenus (Malassezia sp.) fungi of Malassezia restricta and Malassezia furfur all concentrations proportionally superior antifungal activity in respect of the dandruff It was confirmed to have. From the above results, from the excellent antifungal activity of the small bead kohlrabi non-polar solvent extract, the small bead kohlrabi extract was recognized as an excellent dandruff treatment, improvement or prevention effect, the extractables and the solvent are both edible, It has been confirmed that it can be used for a purpose.

In particular, since the small bead coral is obtained from a natural product that can be used as a food material, there are no problems such as side effects of existing antifungal agents or compounds, and it is economical because the small bead coral can be easily obtained from the coast of Korea. In addition, the industrial value will be very large.

Example 3 Preparation of a Hair Composition Comprising Small Bead Coral Extract

Example 3-1 Preparation of Anti-dandruff Shampoo

The anti-dandruff shampoo containing the small bead coral oil ether extract of Example 1 was prepared in the following composition.

3 g of small bead coral oil ether extract of Example 1

30 g of 95% ethanol

0.5 g of surfactant 1 (alkylether sulfate)

0.5 g of Surfactant 2 (sodium lauryl ether sulfate, 2.2 EQ)

2.4 g of kosylbetaine

Water g 100 g

Example 3-2 Preparation of Anti-dandruff Lotion

The anti-dandruff lotion containing the small bead coral oil ether extract of Example 1 was prepared as a solution of the following composition.

1 g of Small Bead Coral Ester Ether Extract of Example 1

30 g of 95% ethanol

Water gs 100g

Example 3-3 Preparation of Cleaning Body Cleanser Composition

The body cleanser composition including the small bead coral oil ether extract of Example 1 was prepared as a solution of the following composition.

2 g of small bead coral oil ether extract of Example 1

18 g anionic surfactant

5 g of nonionic surfactant

7 g of glycerin

3 g of sodium chloride

Natural olive liquid soap 1.5 g

1 g of spices

Water gs 100g

1 is a flow chart showing a process for producing an extract of small bead follicles according to an embodiment of the present invention.

2 is a paper disk for antimicrobial activity against Malassezia furfur , a Malassezia sp. Fungus , a causative agent of dandruff, according to the amount of small bead kohlrabi ether extract according to an embodiment of the present invention. It is a photograph measured by the plate medium diffusion method using. Counterclockwise from top left, with control, 1 mg, 3 mg and 5 mg.

3 is an antimicrobial activity against Malassezia restricta , a Malassezia sp. Fungi, a causative agent of dandruff, according to the amount of small bead kohlrabi ether extract according to an embodiment of the present invention. It is a photograph measured by the plate medium diffusion method using a paper disk. Counterclockwise from top left, with control, 1 mg, 3 mg and 5 mg.

4 is a paper disk for antimicrobial activity against Malassezia furfur , a Malassezia sp. Fungus , a causative agent of dandruff, according to the amount of methanol extract of small bead coral according to an embodiment of the present invention. It is a photograph measured by the plate medium diffusion method using. Counterclockwise from top left, with control, 1 mg, 3 mg and 5 mg.

5 is an antibacterial activity against Malassezia restricta , a Malassezia sp. Fungi, a causative agent of dandruff, according to the amount of small bead kohlrabi methanol extract according to an embodiment of the present invention. It is a photograph measured by the plate medium diffusion method using a disk. Counterclockwise from top left, with control, 1 mg, 3 mg and 5 mg.

FIG. 6 is a paper disk showing antimicrobial activity against Malassezia furfur , a Malassezia sp. Fungi, which is a causative agent of dandruff, according to the amount of small bead kohlrabi water extract according to an embodiment of the present invention. It is a photograph measured by the plate medium diffusion method using. Counterclockwise from top left, with control, 1 mg, 3 mg and 5 mg.

7 is an antimicrobial activity against Malassezia restricta , a Malassezia sp. Fungus , a causative agent of dandruff, according to the amount of the small bead Coral horsetail water extract according to an embodiment of the present invention. It is a photograph measured by the plate medium diffusion method using a paper disk. Counterclockwise from top left, with control, 1 mg, 3 mg and 5 mg.

Claims (8)

Antimicrobial composition against Malassezia sp. Fungi, including Corallina sp. The method of claim 1, wherein the genus Malassezia fungus is Malassezia furfur , Malassezia restricta , Malassezia obtusa , Malassezia globosa ), Malassezia slooffiae , Malassezia sympodialis and Malassezia pachydermatis , wherein the antimicrobial composition is at least one selected from the group consisting of: The antimicrobial composition of claim 1, wherein the algae in the coral is at least one selected from the group consisting of Corallina pilulifera and Corallina officinalis . The antimicrobial composition of claim 1, wherein the seaweed extract of the coral is a crude extract extracted using any one selected from the group consisting of a nonpolar solvent of ether, chloroform, benzene, hexane, dichloromethane, and a mixed solvent thereof. . The method of claim 4, wherein the algae extract of the coral end is fractionated by using the crude extract as a fraction solvent any one selected from the group consisting of a non-polar solvent of ether, chloroform, benzene, hexane or dichloromethane and a mixed solvent thereof. Antibacterial composition that is one fraction. At least one preventive or therapeutic composition selected from the group consisting of dandruff and seborrheic dermatitis comprising the antimicrobial composition of any one of claims 1 to 5 as an active ingredient. At least one preventive or improved cleaning composition selected from the group consisting of dandruff and seborrheic dermatitis comprising the antimicrobial composition of any one of claims 1 to 5 as an active ingredient. At least one preventive or improved cosmetic composition selected from the group consisting of dandruff and seborrheic dermatitis comprising the antimicrobial composition of any one of claims 1 to 5 as an active ingredient.

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