KR100848686B1 - A FERMENTED GINSENG COMPOSITION STRENGTHENED SIMULTANEOUSLY WITH γ-AMINOBUTYRIC ACID AND BIOCONVERSION SAPONIN BY LACTIC ACID BACTERIA - Google Patents

Abstract

The present invention relates to gamma-aminobutyl acid having various physiological activities such as the prevention of senile diseases, and bioconverted saponins having anticancer and anti-allergic activities, that is, compound K, ginsenoside Rh2, ginsenoside Rk1, and ginsenoside Rh1. A method of enhancing the content of gamma-aminobutyric acid and active saponin while fermenting ginseng composition which simultaneously strengthens one or more saponins selected from the group consisting of lactic acid bacteria fermentation technology, and ginseng added with ginseng or glutamic acid (or glutamate, glutamic acid) The present invention relates to food, medicine, and the like.

Ginseng, Red ginseng, Lactobacillus, Fermentation, Saponin, Compound K, Ginsenoside Rh2

Description

Fermented ginseng composition which simultaneously enhanced gamma-aminobutyl acid and bioconverted saponin with lactic acid bacteria {A FERMENTED GINSENG COMPOSITION STRENGTHENED SIMULTANEOUSLY WITH γ-AMINOBUTYRIC ACID AND BIOCONVERSION SAPONIN BY LACTIC ACID BACTERIA}

The present invention relates to gamma-aminobutyric acid (GABA) having various physiologically active effects, such as the prevention of senile diseases, and bioconverted saponins having anticancer and anti-allergic activity, that is, compound K, ginsenoside Rh2, and ginsenosides. The present invention relates to a ginseng composition and a pharmaceutical composition having the above effect, wherein at least one saponin selected from the group consisting of seed Rk1 and ginsenoside Rh1 is simultaneously strengthened using a lactic acid bacterium fermentation technique. The present invention also relates to a method for enhancing the content of gamma-aminobutyl acid and active saponin while fermenting ginseng or glutamic acid (or glutamate, glutamic acid) added ginseng.

Gamma-aminobutyl acid has a molecular formula of H ₂ NCH ₂ CH ₂ COOH, a derivative of an amino acid having a molecular weight of 103.12, and is one of neurotransmitters present in the brain and the like. Gamma-aminobutyric acid improves cerebral blood flow and enhances metabolism, and is effective in mental stability, stroke, head trauma, and cerebral artery restraint. In addition, it lowers blood pressure and prevents high blood pressure (Masashi et al., Nippon Nogeikagaku Kaishi). , 61 (11): 1449-1451 (1987)).

Gamma-aminobutyric acid has recently been used as a pharmaceutical or health food material and is widely known to be widely distributed in plants such as vegetables, fruits, rice, and soybeans, but its content is extremely low and does not show physiological activity. Recently, however, green tea, rice germ, germinated brown rice, etc., having a high gamma-aminobutyl acid content, have been sold in Japan. As a method of increasing the gamma-aminobutyl acid content, a method of increasing the content of gamma-aminobutyl acid in soybean leaves by 20-40 times by cold shock or mechanical stimulation was used.

GABARON tea used in Japan is a green tea (GAYRON, Nippon Shokuhin Kagaku Kogaku Kaishi. 46 (4): 274 ~) that has increased the GABA content by anaerobic treatment (carbon dioxide treatment) of tea leaves with high glutamic acid content. 277 (1999)) and green tea products with increased GABA content (Yusuke et al., Nippon Shokuhin Kagaku Kogaku Kaishi. 46 (7): 462-466 (1999)) were developed. In Korea, a method of increasing the gamma-aminobutyl acid content by anaerobic treatment of green tea leaves for about 12 hours has been developed (Korean Patent Publication No. 1999-0034313).

 In order to increase the GABA content in rice, a high pressure treatment method (Miwako et al., Nippon Shokuhin Kagaku Kogaku Kaishi. 46 (5): 329 to 333 (1999)), and a method of immersing in water (Takayo et al., J. Agric. Food Chem. 42: 1122-1125 (1994).

In addition, when GABA content was increased by anaerobic treatment with gas such as nitrogen dioxide in soybean (Mitsuaki et al., Nippon Shokuhin Kogyo Gakkaishi. 36 (11): 916 ~ 919 (1989)), when GABA content was increased by anaerobic treatment of radish leaves. (John et al., Plant Physiol. 49: 572-578 (1972)), but there is no example of increasing the GABA content by using ginseng.

Ginseng is a perennial root of the genus Ginseng, which belongs to the genus Oga, according to the plant taxonomy. About 11 species of ginseng are known on the earth. For example, Ginseng is native to the Far East of Asia (North 33-48: Korea, North Manchuria, Russia) Excellent Goryeo ginseng ( Panax ginseng CAMeyer); American ginseng ( Panax quinquefolium L.) growing and growing in the United States and Canada; Panax notoginseng FHChen, wild or cultivated in southwestern Guangxi province from southeastern Yunnan Province of China; And Panax japonicus CAMeyer, which are distributed from Japan, southwest China, and Nepal.

Ginseng is not only stored as a commodity in the new agricultural plant, but has been used as a valuable medicine since ancient times. Many pharmacological experiments to date have revealed that ginseng strengthens the nonspecific resistance of the body to stress and has an acidic action. In addition, hypertension, insulin action enhancement, hypoglycemic effect in ALLOXAN diabetic mice, liver RNA synthesis in white rats, protein synthesis, sugar and lipid metabolism promoting effect, and anti-cancer effect.

The ginseng has been used for various diseases such as psychiatric diseases, diseases of the nervous system, and diabetes in the form of herbal medicine mainly in Asian countries such as Korea, China, Japan, and saponins, the main components of the ginseng, are tonic, gangjeong, It is known to have an effect on sedation molding and antihypertension.

Currently, the use of ginseng is red ginseng or ginseng, which is produced by heating white ginseng or ginseng, dried at room temperature, at a temperature of 98-100 ° C., or ginseng, black ginseng, which is prepared by heating at 120-180 ° C. It is used in the form of heat-treated ginseng.

On the other hand, the root of ginseng contains about 4 to 10% of ginseng saponin (Korean ginseng is 4 to 8% and American ginseng is 4 to 10%). The ginseng saponin is a mixture of various ginsenosides, among which Ginsenosides Rb1, Rc and Rg1 are relatively high in Korea, while ginsenosides Rb1 and Re are relatively high in the United States.

The types of ginsenoside components and their pharmacological efficacy contained in ginseng are shown in Table 1 below.

Kinds efficacy    Ginsenoside-Rb1 Central and mental stability, central feeding, aggression, analgesia, anticonvulsion, anti-anxiety, adrenal cortex stimulating hormone and corticosterone secretion, cholesterol biosynthesis, memory improvement, high cholesterol and triglycerides Lowering of fatty acids, promoting neuronal survival, protecting liver injury, promoting DNA, RNA, protein and lipid synthesis of bone marrow cells, promoting the release of acylcholine, vasodilation, inhibiting platelet aggregation, inhibiting lipid peroxidation, promoting cholesterol metabolism, anti-inflammatory, Activation of phagocytosis, inhibition of renal glomeruli    Ginsenoside-Rb2 Promoting sugar and fat metabolism, antidiabetic action, maintaining nitrogen metabolism equilibrium, promoting protein and lipid synthesis, hypocholesterolemic and anti-arteriosclerosis, antagonism of cancer toxin hormone, inhibition of smooth muscle cell proliferation, DNA, RNA, corticosteroids and Promotes corticosterone secretion, improves stress appetite loss, suppresses tumor angiogenesis, promotes the production of antioxidant active substances, activates ATP supply of liver tissues, regulates cholesterol, promotes metabolism of cholesterol, promotes hepatocyte proliferation and DNA synthesis, suppresses platelet aggregation, and analgesic effect Etc Ginsenoside-Rc Promotes liver, serum cholesterol and RNA synthesis, promotes bone marrow cell DNA, RNA, protein and lipid synthesis, analgesic activity, corticosteroid secretion, prostacyclin biosynthesis, renal glomeruli inhibition Ginsenoside-Rd Adrenal cortex stimulating hormone and corticosteroid secretion, renal glomeruli hypertrophy, etc.  Ginsenoside-Re Adrenal cortex stimulating hormone and corticosteroid secretion, analgesic, vasodilation, anti high temperature stress, smooth muscle cell proliferation inhibition, bone marrow cell DNA, RNA, protein and lipid synthesis promotion, liver injury protection, cholesterol metabolism promotion, etc.   Ginsenoside-Rg1 Immune function, platelet aggregation inhibition, antithrombin, good activation, memory and learning function, anti-fatigue, anti-stress, central excitement, vasodilation, anti-inflammatory, anti-nephritis and renal blood flow, high temperature environment and endogenous fever Protective actions against harmful stimuli, improvement of stress-induced slowdown, neuronal survival rate, hepatic cell proliferation and DNA synthesis, adrenal cortex stimulating hormone secretion, cholesterol metabolism, liver protection Ginsenoside-Rh1 Experimental anti-inflammatory effect, tumor cell differentiation promotion, platelet aggregation inhibition, glandular activation activity, anti-inflammatory effect, anti-allergic effect, etc.  Ginsenoside-Rh2 Inhibition of cancer cell proliferation, promotion of cancer cell regeneration, inhibition of cancer cell infiltration, inhibition of tumor growth, enhancement of anticancer activity of anticancer drugs, anti-allergic effect, anti-inflammatory effect, etc.  Compound K Potent tumor angiogenesis and cancer cell metastasis inhibition, type IV collagenase secretion, anti-angiogenic activity and platelet aggregation inhibition, anti-allergic effect, anti-allergic effect, anti-inflammatory effect

As is currently known, ginsenosides, the main constituents of the pharmacological efficacy of ginseng, are saponins of Rb1, Rb2 and Rc. However, the component K (20-O-β-D-glucopyranosyl-20 (S) -proto, which is substantially contained in ginseng, contains a component that substantially inhibits anticancer activity or cancer metastasis of cancer cells or antiallergic action. wave incident diol), ginsenoside Rh1 and Rh2 seed, and △ ²⁰ - it is known that the saponin component of the seeding ginsenoside Rh2 (binary mixture of ginsenosides and ginsenoside seed Rk2 seed Rh3).

Therefore, for use for anticancer action, antiallergic action and immune enhancing action of ginseng, compounds K, ginsenosides Rh1 and Rh2, which are not contained in the ginseng at all or are contained in very small amounts, and Δ ²⁰ -jin It is preferable to increase the content of the saponin component of the senoside Rh2.

Lactic acid bacteria are largely classified into Lactobacillus, Bifidobacterium, Staphylococcus, and Loconosstock strains, and the organic acids produced are slightly different depending on the fermentation type, but they produce lactic acid (lactic acid) in common. In general, the physiological activity of lactic acid bacteria includes inhibition of growth of harmful bacteria, alleviation of lactose intolerance, lowering of serum cholesterol, anticancer activity, immunity, diarrhea and constipation, and vitamin synthesis. Conventional techniques using lactic acid bacteria fermentation are commonly used to inoculate lactic acid bacteria in milk and add skim milk powder to make lactic acid bacteria beverages that have grown lactic acid bacteria.

Fermented food research using lactic acid bacteria includes rice eye drink (Korean Patent Application No. 1996-042486), method of manufacturing staple and liquid soybean oil lactic acid bacteria fermented milk products containing fruit fermented extract (Korean Patent Application No. 1997-01138), Streptococcus Summer Enteral dietary composition comprising Philus and Bifidobacterial long gum (Korean Patent Application No. 1998-0700579), Method of preparing yogurt using wolfberry (Korean Patent Application No. 1997-0065382), Lactobacillus strain Production method of oolong tea or black tea fermented probiotic beverage (Korean Patent No. 150394), Production method of lactic acid bacteria fermented concentrated concentrate (Korean Patent No. 218050), Lactic acid bacteria growth promotion method by adding additives (Korean Patent No. 232907), Lactic acid bacteria Rice Flour Containing Soluble Powder and Method for Manufacturing the Same (Korea Patent Publication No. 1997-0032448), Fermented Brown Rice Flour by Lactic Acid Bacteria And the like product manufacturing method (Republic of Korea Patent Publication No. 1997-0061120 call). However, while producing one or more saponins selected from the group consisting of compounds K, ginsenoside Rh1, ginsenoside Rh2, and ginsenoside Rk1, which have high physiological activities such as anti-cancer and anti-allergic effects of ginseng, It is possible to prepare a bioactive enhanced ginseng composition using the development of the height of the strain, which has not been reported so far.

However, at the same time to increase the content of GABA or increase the content of GABA using ginseng and simultaneously produce ginseng saponins to improve anticancer or cerebral ischemia such as compound K, ginsenoside Rh2, ginsenoside Rh1 or △ ²⁰ -ginsenoside Rh2 Since it is possible to ferment ginseng with lactic acid bacteria, fermentation technology for strengthening compound K and / or ginsenosides Rh2 and GABA can be said to be very useful, and can be used as food for the aging age.

Accordingly, the present inventors also conducted a study to obtain GABA from ginseng more efficiently, and found that the content of GABA was increased by fermentation with lactic acid bacteria, and at the same time, the compound K, ginsenoside Rh1 and ginsenoside Rh2, and △ ²⁰ - ginsenoside seed a result of the studies to increase the saponin component of Rh2 at the same time, the compounds with GABA in the lactic acid fermentation of ginseng obtained sikimeuroseo fermented ginseng as lactic acid K, ginsenoside seed Rh1 and ginsenoside seed Rh2, and △ ²⁰ - found to be true is equivalent to the content of ginsenoside Rh2 oxide and completed the present invention.

Accordingly, it is an object of the present invention to provide a lactic acid bacterium fermentation composition of ginseng or red ginseng having new physiological activities such as liver protection, hyperlipidemia or antioxidant efficacy.

In addition, another object of the present invention is to provide a food such as yogurt containing the lactic acid bacteria fermentation composition.

In addition, another object of the present invention is to provide a lactic acid bacteria used to obtain the lactic acid bacteria fermentation composition.

In one aspect of the invention, ginseng is fermented with lactic acid bacteria to provide a composition wherein the content of gamma-aminobutyl acid is increased.

In addition, the present invention provides a compound K, Δ ²⁰ -ginsenoside Rh2, ginsenoside Rh1, ginsenoside Rh2, protophanacanadiol or protopanaxatriol of the active saponin together with the gamma-aminobutyl acid Provided are compositions with increased content.

In another aspect of the invention, the composition is a composition having hepatic protection, hyperlipidemia or antioxidant efficacy.

In addition, one embodiment of the present invention is a composition made by adding 1 to 5% glutamic acid or glutamate to ginseng and inoculated with lactic acid bacteria to ferment for 1 to 96 hours.

Another aspect of the invention is a composition fermented with lactic acid bacteria using red ginseng in the composition.

In addition, the present invention is a food, such as health food, menopausal and senile foods, beauty foods comprising the composition; Medicines such as tablets and capsules; And it provides processed ginseng related products using them as a material.

The lactic acid bacteria that can be used in the present invention can be any of those that can metabolize ginseng saponin to produce a bioconverter compound K or ginsenoside Rh2, preferably lactic acid bacteria of Bifidobacterium, more Preferably, Bifidobacterium infantis, Bifidobacterium bipidum, Lactobacillus lactis, Clostridium butyricum, Bifidobacterium K-103 (Kim, Kyung Hee University, Dong-Hyun Kim, Arch. Pharm. Res., 21, p 54 ~ 61, 1998), B-Pidobacterium K-506 (Kim, Kyung-Hee University, Dong-Hyun Kim, Arch. Pharm.Res., 21, p 54-61, 1998) BP-Dobacterium K-513 (Kyung-Hee University, Kim Dong-Hyun) Prof. Arch. Pharm.Res., 21, p 54-61, 1998), Bifidobacterium KK-1 (Accession No .: KCCM 10364), Bifidobacterium KK-2 (Accession No .: KCCM 10365) One or a mixed strain thereof selected from the lactic acid bacteria can be used.

The compositions of the present invention may further comprise suitable carriers, excipients and diluents according to conventional methods.

Carriers, excipients and diluents that may be included in the compositions of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, Methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxy benzoate, propylhydroxy benzoate, talc, magnesium stearate and mineral oil.

The compositions of the present invention may be used in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral formulations, external preparations, suppositories, and sterile injectable solutions, respectively, according to conventional methods. .

The amount of the composition of the present invention may vary depending on the age, sex, and weight of the patient, but the amount of 0.1 to 100 mg / kg may be administered once to several times a day. In addition, the dosage may be increased or decreased depending on the route of administration, degree of disease, sex, weight, age, and the like. Therefore, the above dosage does not limit the scope of the present invention in any aspect.

The composition of the present invention may be used in various forms such as drugs, foods and beverages having hepatoprotective, hyperlipidemic or antioxidant efficacy. Examples of the food to which the ginseng fermentation composition can be added include various foods such as various health foods, menopausal and senile foods, beauty foods, beverages, gums, teas, vitamin complexes, and the like. Since the composition has little toxicity and side effects, it is a drug that can be used safely even for long-term administration for the purpose of prevention. The amount of the composition that can be added to the food may generally be added in 0.01 to 15% by weight of the total food weight, the food dry beverage composition is 0.02 to 10 g, preferably 0.3 to 1 g based on 100 ml Can be added in proportion.

The liquid component of the health beverage composition of the present invention is not particularly limited, and may include various flavors or natural carbohydrates, etc. as additional ingredients, as in a conventional beverage. Examples of the natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol, erythritol and the like. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (e.g., Rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of said natural carbohydrate is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (such as cheese, chocolate), pectic acid and salts thereof, alginic acid and salts thereof, Organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the composition of the present invention may include a natural fruit juice and a pulp for the production of fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of about 20 parts by weight or less per 100 parts by weight of the composition of the present invention.

The present invention is described in more detail based on the following examples, although the present invention is not limited thereto.

Test Example 1 Determination of Gamma-Aminobutyl Acid

About 0.03 g of lactic acid bacteria fermentate was added to a tube containing 400 μl of methyl alcohol and accurately weighed, and then methyl alcohol was volatilized for about 2 to 3 hours in a water bath maintained at 55 ° C. 1000 μl of 70 mM LaCl ₃ was added to the sample thus prepared, followed by well mixing and stirring, followed by centrifugation at 11,000 rpm for 5 minutes. Centrifugation yields a supernatant containing gamma-aminobutyl acid. To another tube with 160 μl of 1.0 M KOH, 800 μl of the supernatant was centrifuged and stirred well, followed by centrifugation for 5 minutes at 11,000 rpm. 550 μl of the supernatant, 200 μl of 0.5 MK ₄ P ₂ O ₇ , 150 μl of 4 mM NADP ⁺ 150 μl, and 50 units of 2.5 units GABASE / mL were placed in a disposable cuvette, mixed well, and absorbance was measured at 340 nm. The absorbance was measured and then mixed well with the addition of α-KG. After one hour, the absorbance was measured at 340 nm to calculate the gamma-aminobutyl acid content. This test example was used for the gamma-aminobutyl acid measurement method performed in all the examples, the test example was tested by Guijin et al. (Guojin, Z. and Alan, WB The Rapid Determintion of γ-Aminobutyric Acid.Phytochemistry. 44 (6): 1007-1009 (1997).

Test Example 2 Content Analysis of Saponin Components

1 g each of ginseng and fermented ginseng lyophilisate was extracted, extracted three times with 100 ml of methanol, concentrated, suspended in water, and extracted three times with 100 ml of ether. Then, after extracting three times with 100 mL of butanol, the butanol fraction was concentrated, and the obtained concentrate was dissolved in methanol and analyzed by TLC (as solvent, a lower layer solution of CHCl ₃ : MeOH: H ₂ O = 65:35:10, 5% sulfuric acid solution in MeOH and Shimadzu TLC scanner CS-9301PC were used as a coloring reagent. The content of ingredient was calculated by the content of each ingredient contained in ginseng.

Example 1 Production of Gamma-Aminobutyl Acid and Active Saponin of Ginseng Fermented with Lactic Acid Bacteria

Ginseng extract (3 g dry powder) obtained by drying ginseng extracted at 30-90 ° C, preferably 60 ° C with 70% alcohol (or water), suspended in 100 ml of water and commercially available or newly isolated lactic acid bacteria (wet weight) 10 mg to 3 g, preferably 1 g) is fermented at 37 ° C. for 3 hours to 72 hours, preferably 24 hours and lyophilized to obtain a powder.

Production of GABA and Bioconversion of Ginseng Saponin According to Lactic Acid Bacteria Ginseng Fermented Lactic Acid Bacteria content (%) GABA G-Rb1 G-Rg3 C-K G-Rh2 △ ²⁰ G-Rh2 None Lactobacillus acidophillus L. bulgaricus B. breve K-110 B. longum H-1 B.infantis KCTC 3226 Str. faecalis Leuconstoc mesentroides 0.02 0.03 0.03 0.04 0.03 0.05 0.03 0.03 1.22 0.89 0.92 0.65 0.12 0.34 0.65 0.87 > 0.01> 0.01> 0.01> 0.01> 0.01> 0.01> 0.01> 0.01 0> 0.01> 0.01 0.05 0.43 0.04 0.02 0.02 > 0.01> 0.01> 0.01> 0.01 0.01> 0.01> 0.01> 0.01 > 0.01> 0.01> 0.01> 0.01> 0.01> 0.01> 0.01> 0.01

Fermentation with lactic acid bacteria compared to the non-fermented ginseng resulted in an increase of 50-150% of GABA, an increase in active saponins, and a significant increase in compound K, which was not contained in ginseng.

Example 2 Production of Gamma-Aminobutyl Acid and Active Saponin of Red Ginseng Fermented with Lactic Acid Bacteria

Red ginseng prepared by steaming and drying the ginseng at 90 to 100 ° C, extracted from 50 to 100% alcohol or water, preferably 70% alcohol at 30 to 90 ° C, preferably 60 ° C (3 g dry powder) ) Is suspended in 100 ml of water and fermented and lyophilized at 37 ° C. for 3 hours to 72 hours, preferably 24 hours at a commercial or newly isolated lactic acid bacterium wet weight of 10 mg to 3 g, preferably 1 g. .

Production of GABA and Bioconversion of Ginseng Saponin by Fermented Red Ginseng Red ginseng fermented lactic acid bacteria content (%) GABA G-Rb1 G-Rg3 C-K G-Rh2 △ ²⁰ G-Rh2 None Ginseng Lactobacillus acidophillus L. bulgaricus B. breve K-110 B. longum H-1 B.infantis KCTC 3226 Str. faecalis Leuconstoc mesentroides 0.006 0.007 0.008 0.013 0.010 0.018 0.010 0.009 0.35 0.21 0.19 0.11 0.06 0.09 0.21 0.18 1.4 0.89 0.92 0.49 0.32 0.59 0.91 1.01 > 0.01> 0.01> 0.01 0.01 0.08 0.01 0.01> 0.01 > 0.01 0.02 0.03 0.12 0.52 0.43 0.04 0.05 > 0.01 0.01 0.01 0.03 0.12 0.07 0.01 0.01

Fermentation with lactic acid bacteria compared to unfermented red ginseng resulted in an increase in GABA content by 18-200%, and at the same time an increase in active saponins, especially compound K, which was not contained in ginseng, and ginsenoside Rh2 in trace amounts. , △ ²⁰ - Gene were seeded ginsenoside Rh2 is increased a significant amount.

Example 3 Production of Gamma-Aminobutyl Acid and Active Saponin of Glutamic Acid-Containing Ginseng Fermented with Lactic Acid Bacteria

Ginseng extract (3 g of dry powder) and 1 gram of glutamic acid obtained by drying the ginseng at 30 to 90 ° C., preferably 60 ° C., with 70% alcohol (or water) are suspended in 100 ml of water and sold or newly separated. A lactic acid bacterium is fermented at a wet weight of 10 mg to 3 g, preferably 1 g at 37 ° C. for 3 hours to 72 hours, preferably 24 hours, and lyophilized to obtain a powder.

GABA Production and Glutamic Acid Conversion of Ginseng Saponin by Glutamic Acid Ginseng Fermented Lactic Acid Bacteria content (%) GABA G-Rb1 G-Rg3 C-K G-Rh2 △ ²⁰ G-Rh2 None Ginseng Lactobacillus acidophillus L. bulgaricus B. breve K-110 B. longum H-1 B.infantis KCTC 3226 Str. faecalis Leuconstoc mesentroides 0.02 0.08 0.07 0.12 0.09 0.18 0.19 0.11 1.22 0.95 0.12 0.55 0.12 0.52 0.65 0.97 > 0.01> 0.01> 0.01> 0.01> 0.01> 0.01> 0.01> 0.01 0> 0.01> 0.01 0.06 0.56 0.06 0.02 0.01 > 0.01> 0.01> 0.01> 0.01 0.01> 0.01 0.01> 0.01 > 0.01> 0.01> 0.01> 0.01> 0.01> 0.01> 0.01> 0.01

Lactic acid bacteria fermented ginseng with 1% glutamic acid compared to untreated fermented ginseng increased GABA content from 300% to 850%, and at the same time increased active saponins, especially compound K, which was not contained at all. And back increased considerably.

Example 4 Production of Gamma-Aminobutyl Acid and Active Saponin of Red Ginseng Fermented with Lactic Acid Bacteria

Red ginseng prepared by steaming and drying ginseng at 90 to 100 ° C, extracted from 50-100% alcohol or water, preferably 70% alcohol, at 30 to 90 ° C, preferably 60 ° C (3 g dry powder) ) And glutamic acid are suspended in 100 ml of water and fermented at 37 ° C. for 3 hours to 72 hours, preferably 24 hours with commercially available or newly isolated lactic acid bacteria (wet weight 10 mg to 3 g, preferably 1 g). To obtain a powder.

GABA Production of Glutamic Acid-Contained Red Ginseng and Bioconversion of Ginseng Saponin by Lactic Acid Bacteria Red ginseng fermented lactic acid bacteria content (%) GABA G-Rb1 G-Rg3 C-K G-Rh2 △ ²⁰ G-Rh2 None Ginseng Lactobacillus acidophillus L. bulgaricus B. breve K-110 B. longum H-1 B.infantis KCTC 3226 Str. faecalis Leuconstoc mesentroides 0.006 0.012 0.017 0.108 0.101 0.178 0.162 0.095 0.35 0.29 0.21 0.15 0.05 0.11 0.19 0.18 1.4 0.101 0.95 0.51 0.32 0.49 0.89 1.01 > 0.01> 0.01> 0.01 0.02 0.11 0.01 0.01> 0.01 > 0.01 0.01 0.02 0.11 0.58 0.32 0.04 0.05 > 0.01 0.01 0.01 0.02 0.10 0.05 0.01 0.01

Lactic acid bacteria fermented ginseng with 1% glutamic acid to lactic acid bacteria compared with unfermented red ginseng increased GABA content by 100 to 12,867%, and at the same time increased active saponins, especially compounds K and traces not contained in ginseng. was added ginsenoside Rh2 increased seed significant amounts - that is contained ginsenoside Rh2 seed, △ ^20.

Experimental Example 1. Protective effect of hepatic impairment of fermented ginseng supplemented with GABA and ginseng saponin bioconversion with B. longum H-1

Six groups of rats (male, 180-200 g) were administered orally with 1 ml of 20% carbon tetrachloride (diluted with olive oil) per 100 g, followed by oral administration of ginseng or ginseng fermented product after 30 minutes. 24 hours after administration of carbon tetrachloride, blood was drawn to measure blood aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity.

Dosage (mg / kg) Blood concentration (Karmen unit) AST ALT None - 609 ± 19 203 ± 235 Carbon tetrachloride treated group - 2306 ± 309 ^# 1294 ± 161 ^# Carbon tetrachloride and ginseng treatment group 100 oral administration 1523 ± 238 ^* 825 ± 132 ^* Carbon tetrachloride and ginseng fermentation group 100 oral administration 1154 ± 281 ^* 613 ± 189 ^* Carbon tetrachloride and glutamic acid added ginseng fermented group 100 oral administration 1282 ± 115 ^* 712 ± 167 ^* Carbon tetrachloride and red ginseng treatment group 100 oral administration 1489 ± 216 ^* 712 ± 99 ^* Carbon tetrachloride and red ginseng fermentation group 100 oral administration 1221 ± 145 ^* 809 ± 179 ^* Carbon tetrachloride and glutamic acid added red ginseng fermented group 100 oral administration 1256 ± 298 ^* 735 ± 319 ^* Carbon Tetrachloride And Silymarin Treatment Group 100 oral administration 1344 ± 630 ^* 816 ± 235 ^*

Each value is the mean ± standard deviation (n = 6).

# Significantly different from normal group (p <0.05).

* Significantly different from control (p <0.05).

Hepatic injury of rats with carbon tetrachloride increased blood ALT and AST, but hepatic damage was improved in the ginseng and red ginseng treatment groups. However, as a result of fermenting ginseng or glutamic acid-added ginseng with lactic acid bacteria, soy sauce protection effect was increased compared to ginseng or red ginseng.

Experimental Example 2. Antioxidative Effect of Fermented Ginseng Enhancement of GABA and Ginseng Saponin Bioconversion with B. longum H-1

Radical Scavenging Effect of DPPH (1,1-diphenyl-2-picrylhydrazyl)

500 μl of the sample was added to 500 μl of 60 M DPPH (in EtOH) and reacted at room temperature for 30 minutes, and the absorbance was measured at 520 nm. EtOH was used instead of DPPH as a blank and 100% when water was used instead of the sample as a control to obtain DPPH radical removal rate. Caffeic acid was used as a control drug.

Superoxide Anion Scavenging Effect

To 900 μl of 0.05 M Na ₂ CO ₃ (pH 10.2), 50 μl of 3 mM xanthine (Sigma), 3 mM EDTA (Sigma), 1.5 μg / ml BSA (Sigma), 0.75 mM NBT (Sigma) Each added. 50 μl of the sample and 50 μl of 0.1 μg / ml xanthine oxidase were added thereto, followed by reaction for 30 minutes. 6 mM CuCl ₂ was added thereto to stop the reaction, and the absorbance was measured at 560 nm. Caffeic acid was used as a control drug.

Fermented ginseng, which has GABA enhancement and ginseng saponin bioconverted by fermenting ginseng, has anti-oxidant effect, anti-cancer effect, cerebral ischemia and anti-inflammatory effect.

50% antioxidant effect inhibitory activity (㎍ / ml) DPPH Superoxide Anion Scavenging Caffeic acid (control) 0.4 0.8 Ginseng 125 61 Ginseng Fermentation Group 89 48 Ginseng Fermented Ginseng Treatment Group 71 29 Red ginseng treatment group 96 40 Red ginseng fermentation group 78 35 Glutamic Acid-Added Ginseng Fermentation Treatment Group 68 29

Antioxidant effect of ginseng and ginseng fermentation was recognized by DPPH method and superoxide anion radical sbenzing effect measurement method.

Experimental Example 3. Hyperlipidemic effect of fermented ginseng enhanced with GABA and ginseng saponin bioconversion with B. longum H-1

Blood Lipid Lowering Effects of Corn Oil-induced Hyperlipidemic Mice

Experimental Method: Ginseng or ginseng fermented product was orally administered to 10 rats (male, 20 to 22 g), and the control group was orally administered with saline instead. After 2 hours, 1 g / kg of corn oil was orally administered, and the normal group was orally administered with saline instead of corn oil. Two hours after corn oil administration, light anesthesia with ether was used to draw the heart to triglyceride (hereinafter referred to as "TG"), total cholesterol (hereinafter referred to as "TC"), and low density lipoprotein cholesterol (hereinafter referred to as "low density lipoprotein" (LDL). ) Was measured using Asan Pharmaceutical Co., Ltd. kit.

group Dose (mg / kg / day) TG level (mg / dl) TC level (mg / dl) LDL level (mg / dl) Normal - 84 ± 12 156 ± 13 36 ± 9 Corn oil administration group - 156 ± 25 ^# 165 ± 10 45 ± 13 Ginseng 100 112 ± 16 ^* 148 ± 10 49 ± 18 Ginseng Fermentation Group 100 89 ± 19 ^* 161 ± 19 35 ± 13 Glutamic Acid-Added Ginseng Fermentation Treatment Group 100 82 ± 22 ^* 148 ± 18 36 ± 12 Red ginseng treatment group 100 107 ± 22 ^* 149 ± 12 45 ± 8 ^* Red ginseng fermentation group 100 89 ± 11 ^* 146 ± 14 38 ± 5 Glutamic Acid-Added Ginseng Fermentation Treatment Group 100 82 ± 22 ^* 139 ± 22 39 ± 11 ^* Xenical 10 88 ± 14 ^* 143 ± 17 47 ± 13

Each value is the mean ± standard deviation (n = 10).

# Significantly different from normal group (p <0.05).

* Significantly different from control (p <0.05).

Triglycerides were significantly increased in corn lipids by inducing hyperlipidemia in mice. However, ginseng and red ginseng were recognized to improve hyperlipidemia, but the ginseng fermentation composition, glutamic acid added ginseng fermentation product, red ginseng fermentation product, and glutamic acid added ginseng fermentation product showed better effects.

Blood Lipid Lowering Effect in Triton WR-1339 Induced Hyperlipidemic Mice

Ginseng or fermented ginseng was orally administered once a day for 5 days with 10 mice of 1 group, and physiological saline was administered to the control group. Fasting 16 hours prior to Triton administration and final sample administration 1 hour prior to Triton WR-1339 injection. Dissolve 250 mg / kg of Triton WR-1339 in sterile distilled water and inject the tail intravenously. In the normal group, physiological saline instead of Triton WR-1339 is injected. After 18 hours, light anesthesia with ether is used to draw the blood and TG, TC, LDL. -The amount of cholesterol was measured using Asan Pharmaceutical Co., Ltd. kit.

group Dose (mg / kg / day) TG level (mg / dl) TC level (mg / dl) LDL level (mg / dl) Normal - 79 ± 13 162 ± 9 43 ± 11 Triton WR-1339 administration experimental group - 840 ± 112 ^# 389 ± 17 ^# 89 ± 18 ^# Ginseng 100 621 ± 91 276 ± 15 61 ± 5 ^* Ginseng Fermentation Group 100 555 ± 66 ^* 237 ± 13 ^* 58 ± 10 ^* Glutamic Acid-Added Ginseng Fermentation Treatment Group 100 525 ± 113 ^* 246 ± 22 ^* 49 ± 25 ^* Red ginseng treatment group 100 614 ± 126 ^* 266 ± 25 ^* 67 ± 16 ^* Red ginseng fermentation group 100 540 ± 72 ^* 232 ± 20 57 ± 9 ^* Glutamic Acid-Added Ginseng Fermentation Treatment Group 100 592 ± 89 ^* 229 ± 22 ^* 52 ± 12 ^* Lovastin 10 559 ± 94 ^* 287 ± 76 57 ± 11 ^*

Each value is the mean ± standard deviation (n = 10).

# Significantly different from normal group (p <0.05).

* Significantly different from control (p <0.05).

Triton WR1336 induced hyperlipidemia in mice and measured blood lipid levels, which significantly increased TG, TC, and LDL-cholesterol. However, ginseng and red ginseng were recognized to improve hyperlipidemia, but the ginseng fermentation composition, glutamic acid added ginseng fermentation product, red ginseng fermentation product, and glutamic acid added ginseng fermentation product showed better effects.

As described above, the ginseng or glutamic acid-containing ginseng is fermented using lactic acid bacteria through the technical composition of the present invention to convert to gamma-aminobutyl acid having dementia-preventing effects such as brain metabolism enhancement effect and at the same time, antioxidant activity and liver protection. Lactic acid bacteria fermented ginseng composition having the effect, such as anti-hyperlipidemia can be made. Through this invention, lactic acid bacteria fermented products can be used not only as lactic acid bacteria, but also variously used, such as health food, menopausal and senile foods, women's food, beauty food materials and other products.

Claims (8)

Bifidobacterium infantis, Bifidobacterium bifidum, Lactobacillus lactis, Clostridium butyricum, Bifidobacterium K-103, Bifidobacterium K-506, Bifidobacterium K-513, Bifi Ginseng was fermented with a lactic acid bacterium selected from the group consisting of Dobacterium KK-1 and Bifidobacterium KK-2, together with gamma-aminobutyl acid, Compound K, Δ ²⁰ -ginsenoside Rh2, ginsenoside Rh1, A pharmaceutical composition for the prevention and treatment of hyperlipidemia, cancer, cerebral ischemia or inflammation, comprising ginsenosides Rh2, protophanacanadiol, and active saponins having an increased content of at least one selected from the group consisting of protophanacanatriol. delete delete Bifidobacterium infantis, Bifidobacterium bifidum, Lactobacillus lactis, Clostridium butyricum, Bifidobacterium K-103, Bifidobacterium K-506, Bifidobacterium K-513, Bifi Fermented red ginseng with lactic acid bacteria selected from the group consisting of gambacterium KK-1 and Bifidobacterium KK-2, together with gamma-aminobutyl acid, compound K, Δ ²⁰ -ginsenoside Rh2, ginsenoside Rh1, A pharmaceutical composition for the prevention and treatment of hyperlipidemia, cancer, cerebral ischemia or inflammation, comprising ginsenosides Rh2, protophanacanadiol, and active saponins having an increased content of at least one selected from the group consisting of protophanacanatriol. delete delete Bifidobacterium infantis, Bifidobacterium bifidum, Lactobacillus lactis, Clostridium butyricum, Bifidobacterium K-103, Bifidobacterium K-506, Bifidobacterium K-513, Bifi Ginseng was fermented with a lactic acid bacterium selected from the group consisting of Dobacterium KK-1 and Bifidobacterium KK-2, together with gamma-aminobutyl acid, Compound K, Δ ²⁰ -ginsenoside Rh2, ginsenoside Rh1, Health food comprising an active ingredient saponin of increased content of at least one selected from the group consisting of ginsenoside Rh2, protophanakanadiol and protophanakanatriol. Bifidobacterium infantis, Bifidobacterium bifidum, Lactobacillus lactis, Clostridium butyricum, Bifidobacterium K-103, Bifidobacterium K-506, Bifidobacterium K-513, Bifi Ginseng was fermented with a lactic acid bacterium selected from the group consisting of Dobacterium KK-1 and Bifidobacterium KK-2, together with gamma-aminobutyl acid, Compound K, Δ ²⁰ -ginsenoside Rh2, ginsenoside Rh1, Prevention and treatment of liver damage, hyperlipidemia, cancer, cerebral ischemia or inflammation, comprising as an active ingredient an active saponin with an increased content of at least one selected from the group consisting of ginsenoside Rh2, protophanacanadiol and protophanacanatriol Pharmaceutical products that are tablets or capsules for.