KR100812968B1 - A study of purifying hunman inner body with natural herbal plants - Google Patents

Abstract

A natural herbal composition is provided to remove harmful ingredients accumulated in human body due to smoking by extracting various herbs effective for eliminating harmful ingredients from the human body, concentrating, purifying and fermenting the extract. A method for preparing a natural herbal composition for detoxicating human body caused by smoking comprises the steps of: (a) preparing each 10kg of each dried powder of Houttuynia cordata thunb, Geranium nepalense Sweet, Glycine max (L.) Merrill, Plantago asiatica L., Solanum nigrum, Pueraria thunbergiana Benth, Lonicera japonica Thunb, Glycyrrhiza glabra L., Gardenia jasminoides Ellis, Schizandra chinensis Baillon, Eucommia ulmoides, Dioscorea Rhizome and Smilacis Chinae Radix, respectively; (b) mixing 10 times of purified water, C1-4 alcohol or a mixture solvent thereof regarding each of the dried powder and agitating for 5 hours at a temperature of more than 75 deg.C for the Houttuynia cordata thunb, for 12 hours at a temperature of 25 deg.C for the Gardenia jasminoides Ellis, and for 5 hours at a temperature of 95 deg.C for the other remaining powders, respectively; (c) after respectively centrifuging each of the products obtained from the step(b) and removing solid residuals therefrom to obtain a filtrate, leaving the filtrate for 1 day and then filtering supernatant therefrom; (d) concentrating each of the extract obtained from the step(c) under the pressure of 70-76 mmHg at a temperature of 55 deg.C to obtain each of solid ingredients; (e) concentrating each of the solid ingredients of the Houttuynia cordata thunb, Geranium nepalense Sweet, Plantago asiatica L., Pueraria thunbergiana Benth, Lonicera japonica Thunb, Glycyrrhiza glabra L., Gardenia jasminoides Ellis, Schizandra chinensis Baillon, Eucommia ulmoides, Dioscorea Rhizome and Smilacis Chinae Radix to have the solid content of 60%, concentrating the solid ingredient of the Glycine max (L.) Merrill to have the solid content of 40%, and concentrating the solid ingredient of the Solanum nigrum to have the solid content of 50% and obtaining 6-7 kg of an extract for each of the Houttuynia cordata thunb, Geranium nepalense Sweet, Plantago asiatica L., and Gardenia jasminoides Ellis, 4-5 kg of an extract of each of Plantago asiatica L., Pueraria thunbergiana Benth, Eucommia ulmoides, Dioscorea Rhizome and Smilacis Chinae Radix, and 5-6 kg of Solanum nigrum, Glycyrrhiza glabra L., and Schizandra chinensis Baillon; and (f) mixing each of the extracts obtained from the step(e) with agitation and fermenting the mixture at a temperature of 30-40 deg.C for 40 days.

Description

Herbal herbal composition having an effective effect on eliminating toxic substances in the human body due to smoking and a method of manufacturing the same {A Study of Purifying Hunman Inner Body with Natural Herbal Plants}

Figure 1 shows the proliferation effect by spleen cells

Figure 2 shows the mutagenicity results of 2-Aminofluorene (2-AF)

Figure 3 shows the results of quantitative analysis of NNN, NAT, NNK, B (a) P in the tar of tobacco.

The present invention improves the conventional smoking-related herbal composition to propose a new composition different from the conventional composition to obtain a herbal composition with better efficacy in removing toxic components in the human body due to smoking and at the same time using the above composition It is to be able to provide a method for easily manufacturing a herbal medicine.

In general, a decrease in the human body due to smoking refers to the conversion of a toxic substance into a non-toxic substance in cells in a living body, that is, a constant biochemical reaction in which the body converts a structure that is less actionable or easy to excrete.

The environment that surrounds life varies from simple molecules like oxygen to large molecules like proteins. All of these substances, if appropriate, are needed or harmless to the cells. However, if the amount is above a certain amount will inhibit the normal function of the cell.

The amount at which a substance is harmful to cells depends on the type of organism, but is nearly equal for all kinds of biological cells.

Detoxification therapy is a treatment that detoxifies harmful toxins from inside the body.

People are exposed to thousands of toxic chemicals and pollutants of water and soil every day in their daily lives. These substances themselves cause a number of symptoms, including immunity, neurotoxicity, hormonal side effects, mental problems, and even cancer.

Even when exposed to so many risk factors, the human body has a natural effect of releasing toxic substances and waste products through the kidneys, liver, urine, feces and respiration.

Since the Industrial Revolution, however, many chemicals, insecticides, various drugs, food additives, heavy metals, anesthetics, various drugs, and legal substances such as alcohol, tobacco, caffeine, and various substances such as illegal drugs are exposed. There is a limit to the function of the excretion process.

Today's people are exposed to a much larger amount of chemicals than their predecessors, and the condition of the disease is complicated and chronic, resulting in immunocompromised, neurological, hormonal, mental and cancer diseases.

Therefore, many studies have been conducted on various useful compounds that may contribute to effective toxicity reduction and defense ability protection against the exposed risk factors, but the development of remarkable functional additives has not been made.

In addition, there are many substances and elements that harm people's health, but the most damaging is tobacco. Tobacco is the biggest cause of death in the modern age.

The research on the association between smoking and disease has been intensively studied since the mid-1900s, and there is a continuous evidence of increasing incidence of various diseases including lung cancer caused by smoking and other health disorders.

In fact, about 4,000 kinds of components were found in cigarette smoke as early as present, and it is estimated that there are more than 100,000 kinds of components in mainstream smoke. Among them, nicotine shows the effects of all nicotinic receptors in autonomic nervous system. In combination it causes excitement in all sympathetic, parasympathetic nerves.

However, this excitement does not occur continuously, and when a large amount of nicotine is supplied, the time of excitation is very short, and for the central nervous system, nicotine is initially excited as in the autonomic nervous system and later paralyzed.

That is, the reflex excitability of the spinal cord is increased, each center of the training (excited nerve center, respiratory center, etc.) is excited to slow the pulse, faster breathing, tremor appears, and then the whole body spasms.

Following the excitement action, the paralysis enters the end of the respiratory center and peripheral respiratory paralysis may die, and if you smoke too much at once is the cause of death.

In the circulatory system, blood vessels and blood pressure predominantly affect the function of the vascular movement center and sympathetic nervous system, which causes blood vessels to contract and increase blood pressure, but as time passes, the blood pressure decreases again. When people with the disease smoke, blood vessels shrink and narrow, which makes the blood vessels worse, resulting in myocardial infarction or ischemia.

Nicotine also causes paralysis of the respiratory center and respiratory muscles, hyperactivity and inhibition of various glands in the body, conjunctivitis, atrophy of the optic nerve, and degeneration of the optic ganglia resulting in so-called tobacco amblyopia and stimulating parasympathetic nerves in the digestive system. Increasing bowel movements, nausea, vomiting, severe diarrhea, and nicotine is known to die when 1mg per 1kg of body weight, corticosteroids, free fatty acids and antidiuretic hormones in the blood to increase blood sugar.

In addition, nicotine acts as a very potent carcinogen by nicotine derived nitros amine, which has resulted in hundreds of millions of lung cancer patients worldwide every year from smoking, and in 1956 Magee and Barnes reported nitrosodimethylamine (N'-). Nitrosodimethylamine (NDMA) proved to be a very potent liver cancer-causing agent in mice (Magee, PN, and Barnes, JM (1956) Br. J. Cancer, 10, 114-122), after which more than 200 N-nitrosamines More than 30 compounds with carcinogenicity were identified.

Smith and others showed that various nitrosamines were formed from nicotine, and Hecht demonstrated that many other substances, such as NNK, NNA, and NNN, were formed from nicotine.

And people who smoke tar are often said to have a taste of tobacco, which means that each cigarette enjoys a unique tar scent, such as gin that flows when burning burning grass.

The fact that smoking is harmful to the body is the same as saying that tar is harmful to the body. The most harmful ingredient in cigarettes is tar.

Tar plays the most important role in cancer, and cigarettes contain more than 1,200 cancer-causing agents, most of which belong to tar. Most of the carcinogenic substances in tar are proteinaceous nitrogen, which causes cancer not only in the respiratory system but also in many parts of the body, especially in the urine, thus increasing the incidence of bladder cancer. Nevertheless, it is not easy for a cigarette lover to quit smoking habits, and there is no easy means and method to prevent various diseases due to smoking while continuing to smoke.

The present invention has been invented with the technical problem to propose a herbal medicine that can remove, reduce or detoxify toxic components in the human body accumulated due to smoking, and the present invention has been used as a herbal medicine and food for a long time in Korea and China. In addition, among natural herbal medicines that have been shown to reduce the toxicity of tobacco (nicotine, tar, dioxin, etc.), three hundred seconds, algae grass, black bean, chajeoncho, yonggyu, brown root, tofu, bokbokyeong, mountain medicine, honeysuckle, licorice, gardenia, It is intended to solve the technical problem of the present invention by providing a herbal composition and a method of manufacturing the main ingredient of Schisandra chinensis.
This will be described in more detail as follows.

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The present invention contains the ingredients obtained from 1 to 10 parts by weight, respectively, three hundred seconds, lichen grass, black bean, chajeoncho, yonggyu, brown root, tofu, Tobokyeong, powder, honeysuckle, licorice, gardenia, Schisandra chinensis, as known To provide a herbal composition and a method for preparing the composition, the following is a description of the method of preparing a composition using the natural herbal medicine, after first looking at the components and normal efficacy of the natural herbal medicine, but the efficacy is obvious A description of one component will be omitted.

Three hundred seconds ( Houttuynia cordata thunb ( poly cordata Bueck ) mainly uses the stem and leaves of the plant, and the main components are methylnonylkenone CH ₃ (CH ₂ ) ₈ COCH ₃ (oxidation product of decanoyl acetaldehyde) myrcene (C ₁₀ H ₁₆ ), laurin aldehyde (C ₁₁ H ₂₃ CHO), caprinaldehyde (C ₉ H ₁₉ CHO), capric acid, and many other components and leaves contain quercitrin and minerals released in water. Tenfold concentration) and cardiac activity, and antibacterial and capillary potentiation effects against E. coli, typhoid, paratyphoid, erythropoietic, gonococcus, staphylococcus, and filamentous fungi. It has antimicrobial activity against Staphylococcus aureus, gonococcus, and acidophilic bacteria.

Its applications are inflammatory drugs and diuretic poisoning drugs, which are used for gonorrhea, urethritis, cystitis, uteritis, pneumonia, bronchitis, water pooling, athlete's foot treatment, antidepressant, swelling, windworm, sinusitis.

Geranium nepalense Sweet (G. thunbergii Siebold et Zuccarini ) uses the young leaves of plants, the main components of which are pyrogalloltanine, glutenous acid, succinic acid, quercetin and its glycosides, and camphorol and kemperitriline C ₂₇ H ₃₀ O ₁₄ · 3 1/2 H ₂ O (D'rhamnoside from Camperol), and other sources show tannins based on geranine, a type of ellagtanine.

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As a main action, the water extract or alcohol extract of the outpost increases intestinal tension. Diarrhea is as clear as bismuth preparation, and no amount of side effects, even if you use a large amount of rice, do not lose the taste, bactericidal bacteria, typhoid fever, E. coli bactericidal action.

Heukdu (黑豆) (Glycine max (L. ) Merrill) is another name in the seoritae, as called beans, soybean components has said protein is 36-45%, the oil contains 17-22% Other tree pepper Terre There are nooids saponins, nitrogen compounds, carbohydrates, candies, pectins, minerals and vitamins.

80 ~ 90% of protein consists of essential amino acids such as arginine, methionine, threonine, leucine, isoleucine, valine, etc.Phospholipid content includes lecithin 1 ~ 3.5%, kephalin, and nitrogen compounds such as adenine, guanine, choline , Trigonelin, and the amino acid histidine.

Carbohydrates are made up almost completely of water, including 3.3-13% saccharose.

The ash contains K, Mg, Cu, Fe, Mn, Ni, Zn and traces of Co and phosphoric acid, and vitamins A, B ₁ , B ₂ , D, E, K and isoflavone glycosides such as diazine, heistin, and rovinin. This contains.

Where it works, soy saponin is active in lipid metabolism. Lecithin in soybeans emulsify fats and steroids, which are not easily soluble in water, so that they are released in water, thereby reducing the amount of cholesterol, the main substance that causes atherosclerosis.

Lecithin also breaks down the body into choline, glycophosphate, and fatty acids, which have a beneficial effect on neurophospholipid metabolism.

It is used as a raw material to make drugs that stimulate the central nervous system, drugs to treat diabetes and light diseases, and is good for excessive secretion of gastric juice, acute and chronic infectious diseases, and typhoid fever.

In motion therapy, anti-inflammatory stomachache, digestive medicine, fever lowering medicine and sweating medicine are used in the early stages of fever and when the head is sore and cold.

Chachocho ( Plantago asiatica L. (P.major L. var. asiatica Decne ) is used to cut the bottom of leaves and dry them in the sun.The leaves contain aquabin, an iridoid glycoside (C ₁₅ H ₂₄ O ₉ ). Catalanpol and flavonoids, lantaginine (scutellalane-7-glucoside), homoplantaginine (hispitolin-7-glucoside) and apigenin, luteolin, nefetin, 6-oxyluteolin glycosides, and Ursolic acid, gentriacontane (C ₂₃ H ₄₈ ), palmitic acid esters of β-sitosterol, stigmasterol and β-sitosterol.

There are also enzymes (emulsine and inverotene), carotene and ascorbic acid, and seeds have acubin and a lot of mucus.

Partial hydrolysis of the mucus resulted in the disaccharide I [O-β-D-xyclopyranosyl- (1 → 2) -D-xyclopyranose], И [O-β-D-xyclopyranosyl- (1 → 4) -D-xyclopyranose] and III [(-β-D-glucopyranosyl-uronic acid- (1 → 5) -L-arabinopyranose)]. Others include planthenol acid, succinic acid, choline, adenine, vitamins A and B ₁ .

Outposts have polysaccharides that are released in water. Polysaccharides contain up to 28% ash, and only about half are removed by electrodialysis. If refined for a long time, the ash reaches 0.4 ~ 0.5%. Polysaccharides consisted of 80-82% pectinic acid, 5-6% galactoaraban and 4-5% galactan. Pectinic acid produces 78-83% of galacturonic acid, galactose, arabinose, rhamnose, small amounts of glucose, xylose and three other sugars. In other words, the polysaccharide consists of all nine sugars and minerals.

Where it works, outposts and planttagins act on the respiratory centers in animal experiments, slowing and deepening breathing. It also increases the secretion of mucous membranes, increasing the secretion of bronchial mucosa and digestive juices.

Seed mucus is a relaxing and heat-lowering effect.

Polysaccharide component is anti-inflammatory, epithelialization promoting action.

Outpost extracts have anti-inflammatory and epithelial activity in small wounds in animal experiments.

Feeding polysaccharides in rats 1 to 3 g / kg and dogs to 2 to 5 g / kg for 14 days has no toxic effects and suppresses butadione-induced gastric ulcer formation in rats.

Feeding dogs also increases the secretion of gastric juice by 2.5 times, increases the free acidity and total acidity of gastric juice by 20-30%, and slows the movement of material through the stomach by 3-4 times.

The hardening effect of polysaccharides on isolated intestines of rats is also shown in 1: 10,000 solution.

As such, polysaccharides have a hardening effect, anti-inflammatory action, and epithelialization promoting action, so it is a good medicine for gastric ulcer.

The functional properties of polysaccharides lower the acidity and increase the acidity to normalize the secretion of gastric juice, especially in patients with normal or low acidity of gastric juice.

Applications include polysaccharides or fresh juices derived from outposts for gastric juices with normal or low gastric duodenal ulcers and chronic gastritis.

Outpost decoction is also effective for nephropathy.

In motion therapy, outposts and seeds are used to remove moist heat and brighten the eyes, so they are used as eye diseases, cystitis, jangkata, pissing, diarrhea, and inflammatory drugs, urinating drugs, and diarrhea. In addition, it is used as a cough medicine for respiratory diseases such as white cough and bronchitis. In civilian use, outposts are used for gastrointestinal diseases and other arteriosclerosis and diabetes. Mr. Moon drinks from eye diseases and nervous system diseases.

Solanum nigrum uses the fruit of plants.

 Ripe fruits include glycoalkaloids, saponins and anthocyanin pigments, β-carotene 1,630 mg% ascorbic acid, solasonin (0.2%) and solamargin (0.25%).

It acts as an inhibitory effect against typhoid bacteria, staphylococcus aeruginosa, Pseudomonas aeruginosa, E. coli, coliform, and fungi.Alkaloids promote histamine breakdown in lung and bronchial tissues. Protects pulmonary function

The application is to restore fatigue from motion therapy to tonic, fever and urinary medicine.

In the private sector, it is used as a tonic medicine and urine medicine for nephropathy and bite water, and vinegar juice is used as a sedative medicine, jingyeong medicine, and sweat medicine for cold.

Pueraria thunbergiana Benth is another name called bush and vine, and the used part is used by digging in the early spring or late autumn and washing it with water.

Component in the roots at the isoflavone compound daidzein _{(C 15 H 10 O 4)} , daidzin (C ₂₁ H ₂₀ O _9), the rarin (C ₂₁ H ₂₀ O ₉₎ to Pu, Fu rarin xylose Seed, luteolin, biocanine A (C ₁₆ H ₁₂ O ₅ ) and the like, there is a horse, coumarin. In addition, choline, acetylcholine-positive substances, cacaine, and puerarol cracked.

Where it works, feeding the root powder 15g / kg to the heated rabbit stimulates the warming, but there is a distinct heat-lowering effect but no other special change.

The isoflavone compound in roots has a hardening effect.

In particular, this action is strong in dydzein. Cirrhosis is due to papaverine and antiacetylcholine action.

Isoflavones are thought to have been used for the soreness of the roots as a medicinal agent in the synergistic treatment.

 According to an example of using dyedzein for various heart failures such as migraine, hypertension, and angina pectoris, cardiac extension of the heart was effective in 70-80% of patients.

Thus, dyed jane is used for hypertension, migraine and angina. At the root, there is a substance that antagonizes dyspane's dystrophic action, that is, a substance that strongly contracts the smooth muscle organs.

It is used in the treatment of motion in the roots and flowers are used for fever with antipyretic fever and sensitizing medicine. When the throat is dry and the head hurts, the cold and the head and neck hurts, tonsillitis and acute otitis media.

It is also used for small head sickness, fever and vomiting, pain in the head and stuffy stomach, when the neck and shoulders are stretched out, to loosen the blood and heal the wound, and the leaves are used for the head pain that comes with high blood pressure. Also used, the root is used for high blood pressure, explosive ear.

Honeysuckle (Lonicera japonica Thunb) cuts the stems of the plant's leaves like a round skein, winds it up and dries it in the sun, then uses the stem and leaves.

Ingredients include 5-8% tannins in the leaves and stems, loganine, about 19% nitrogenous content in the leaves, and roniserine, a luteolin-7-rhamnoglucoside (C ₂₇ H ₃₀ O ₁₅ 2H ₂ O).

Water decoction acts as a pissing action and increases blood sugar, and also has a fungal effect on erythrocytes, Typhoid bacteria, staphylococci, and pneumococci. 예방 There is also a protective effect of peptic ulcer, sympathetic nerve excitement, smooth muscle paralysis.

It is used in motion therapy for flowers, leaves, and stems when it is opened early in Pung-kak with heat-lowering medicine, poisonous medicine, urinary medicine, and blood shaping medicine.

Licorice ( Glycyrrhiza glabra L.), in the fall, cut off the rhizome and roots, trim the stems, wash them in water and use them to dry in the sun.

Roots and stems contain 5-14% glycyrrhizine (potassium or calcium salt of glycyrrhizin acid) (23% more) and glabraic acid (glyciretin) (C ₃₀ H ₄₆ O ₅ ).

Glycilisic acid (C ₄₂ H ₆₂ O ₁₆ ) is triterpenoids saponin, melting point 205 ℃, decomposition point 220 ℃, [α] D ²⁰ + 58.5 °, well soluble in alcohol and hot water, not soluble in ether. After cooling in hot water, it hardens like grass.

Decomposition of water results in tasteless glycyrrhetinic acid (C ₃₀ H ₄₆ O ₄ ) and two molecules of glucuronic acid (C ₆ H ₁₀ O ₇ ).

Glycyretinic acid is a β-amirin-based triterpenoid, which has two kinds of α and β substances, which are easily changed.

α-glyciretinic acid is a plate-like crystal (dilute alcohol), melting point 283 ° C., [α] D ²⁰ + 140 ° (ethanol), soluble in ethanol, dioxane and chloroform.

β-Glycyretinic acid is needle crystal (ethanol), melting point 296 ~ 300 ℃ [α] D ²⁰ + 86 ° (alcohol), soluble in ethanol, pyridine, acetic acid and insoluble in petroleum ether.

In addition, flavono glycosides, liqueurin (C ₂₁ H ₂₂ O ₉ ), neoriquirithins, and rhamnoricuritin (all of which are non-glycosides are liqueurinigen) and their corresponding galcon glycosides, isoricuitin , Neoisorikuuritin, isoram norikuuritin, and liqueuride (the non-sugar part of these are all Isorikuuritingenin).

More than 27 flavonoid analogs were identified by chromatograph in licorice root.

Β-sitosterol, formmononetin, ricolycidine, lycoricone, and ricore-oligonane were identified in the curved licorice roots.

Glycyrrhinic acid produced by water decomposition of glycyrrhizin, known as an active ingredient of licorice root, has no sweetness and has a hemolytic action.

Licorice root extract and glycyrrhizin are non-toxic and can relieve poisoning caused by po catchochloral, strycnin, yohimbine, cocaine, morphine, codeine, atropine and luminal.

It releases bacterial and viral poisons such as tetanus toxin, diphtheria toxin and snake venom. It also antagonizes with acetylcholine, histamine and allergens, and has an adrenaline-like effect.

The poisonous action of glycyrrhizin is related to glucuronic acid, a product of water degradation.

Glucuronic acid is a component widely found in animal and plant systems and is combined with toxic substances in the liver to form glucuronide and excreted by urine.

Therefore, glucuronic acid is used for drug addiction, alcoholism, food poisoning, jaundice, cirrhosis of the liver, chronic liver, rheumatism, joint pain, nerve pain.

The detoxification of glycyrrhizin is not thought to be caused solely by glucuronic acid, but is also related to the action of glycyrazine similar to that of corticosteroids.

Glycirizine has the same effect as desoxycorticosterone, the corticosteroid. Glycyretinic acid reduces sodium, chlorine, and urine excretion, increases potassium excretion, and lowers the Na / K ratio in animal studies.

As such, it negatively affects potassium metabolism but positively affects sodium metabolism.

It also acts to reduce the ascorbic acid content of the adrenal cortex, which sets conditions to enhance the effects of corticosteroids on water and salt metabolism.

Licorice root extract glycyrrhizin is anti-inflammatory. In other words, it positively affects the treatment of experimentally induced formalin arthritis in animals, inhibits the activity of serum transaminase and increases the activity of adenosine triphosphatase in the liver.

The anti-inflammatory action of glycyrrhizin similar to desoxycorticosterone is believed to be due to the degradation product glycyrrhetinic acid.

Licorice root extract has a fascinating muscular dystrophy similar to papaverine on gastrointestinal smooth muscle in animal experiments. This action is due to the flavonoid component.

Licorice flavonoids, which have not been used until now, was developed with known antifungal and anti-inflammatory effects. Licuraside, a flavonoid component, has three times as strong as papaverine and has anti-inflammatory and anti-ulcer activity. And antagonists of acetylcholine and histamine.

Licorice has a strong surface activity, so if you eat it with other copper medicines, it helps loosen the ingredients in water to help absorption. It also reduces the toxicity of the medicinal herbs and changes the taste. Licorice root has a slight inhibitory effect against Staphylococcus aureus, Escherichia coli, and Lycobacterium. When glycyrrhizin is used with anti-cancer drug thiopoxamide, anti-cancer activity reaches 85-95%.

Its applications include sputum sputum, mitigating, anti-inflammatory and antifungal and antihistamine. Therefore, sputum acupuncture is used as an upper respiratory tract disease, because it has a sweet taste, it is also used as a drug medicinal acupuncture and poison medicine or excipient.

Recently, it is widely used for gastroduodenal ulcer, Edison's disease, bronchial asthma, jaundice and hepatitis, eczema and other skin diseases.

In motion therapy, the roots are used mainly for the purpose of alleviating and harmonizing the actions of other drugs. In other words, when used with hot medicine to relieve heat, and when used with cold medicine to relieve cold.

As a palliative or pain 멎 jinjak, sputum pill, cough 멎 yipo, poison pool medicine stomach cramps, stomach pain, sore throat, stomach ulcers, duodenal ulcers, etc. and enters the poisonous prescription.

Gardenia (Gardenia jasminoides Ellis) consists of iridoid glycosides, genipine (C ₁₁ H ₁₄ O ₅ ), its glycosides, geniposides (C ₁₇ H ₂₄ O) and genthiobiosides, gardenides and There is a yellow pigment, α-crochin. In addition, there are coumarin ingredients such as xantholeicin, praserene, and feudanol methyl ester.

Where it works, the water and ethanol extract of the fruit does not significantly increase the secretion of heat when fed to the rabbit, but inhibits the bilirubin out into the blood and peripheral forest during total bile duct ligation. When crocin and its sodium salt are injected into the rabbit's vein, it increases heat secretion and suppresses the blood of the rabbit, which holds the total bile duct. Indole alkaloids lower blood pressure. Feeding the fruit to white mice causes mild diarrhea, the action of which is geniposide.

Applications include anti-inflammatory, sedative, urinary, fever-lowering, blood-repellent drugs in motion therapy, inflammation, bleeding, myocarditis, hemostasis, keratosis, and jaundice.

Schisandra chinensis (Schizandra chinensis Baillon = Schisandra chinensis ) picks ripe berries in the autumn and uses them to dry in the sun and then in the shade.

Ingredients include 45% to 47% alcohol, 39% to 41% water, and organic acids.

The organic acid content of the fruit, including seeds, is 10.9-12.8% of lemon acid, 7.6-10% of malic acid, and 0.8% of grape acid. The fruit also contains 7.8% resin, about 5% saponin, 350-762 mg% ascorbic acid, and the seeds contain 20-30% oil, resin, 1.6-25 essential oils, and lignin compounds.

The skin has a lemon-like odor and is a full yellowish color with about 20% aldehydes and ketones.

It acts as a rainforest medicine and tincture of the fruit has a long-lasting excitatory effect on the central nervous system and remarkably enhances reflex excitability.

In addition, it increases the excitability of the muscle nerve, improves the function of the peripheral nervous system and has a good therapeutic effect on the cardiovascular system.

Suzandrarin increases the reflex excitability of the spinal cord, has a strain on the cardiac blood system and breathing, helps carbohydrates and ligaments, speeds up tissue breathing, increases the activity of enzymes, inhibits arteriosclerosis, and lowers enzyme units during hepatitis. There is also.

Clinical trials have a marked therapeutic effect on systemic weakness, nervous breakdown, systemic schizophrenia and hypotension.

Schizandra chinensis alcohol extract lowers serum glutanic acid acetate transaminasase (SGPT) concentration, which is increased due to drug addiction in animal experiments, strengthens liver detoxification function, and promotes liver glycogen production and serum protein synthesis. Clinically, it has some therapeutic effects on chronic viral hepatitis and drug hepatitis, and the effect of lowering SGPT is obvious.

Applications include systemic weakness, mental and physical fatigue, nervous breakdowns, hypotension, heart failure, malnutrition ulcers, and to improve vision.

In addition, clinical reviews have shown that fruit and seeds can significantly improve laboratory test indicators when used in epilepsy.

In motion therapy, cough, astringent, astringent, and nourishing tonic are used to protect the lungs and to help the kidneys. Cough, dry mouth, diarrhea and sweating and sputum are often used. In case of high liver dysfunction, sputum pee urine and juice are used as a potent tablet in oil wells.

Each extraction method of the active ingredient extract of the herbal medicine to obtain the herbal composition with the above ingredients and efficacy as shown in the present invention is as follows.
Three hundred seconds;

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10 kg of low alcohol (50% ethanol) of C _1-4 was added to 10 kg of the dry powder of 300 seconds, and extracted with gentle stirring for 5 hours while maintaining 75 ° C or more.

The obtained product is centrifuged, the solid residue is discarded, the extract is left for 1 day, and the supernatant is filtered and concentrated under reduced pressure.

The concentration under reduced pressure was concentrated at 55 ° C. under reduced pressure (70-76 mmHg), and the obtained solid component was concentrated to a solid content of about 60% and 6 kg-7 kg was obtained as an extract. The extract is aged and fermented (when fermentation fermentation is carried out for 40 days while maintaining the temperature of 30 ° C. to 40 ° C.) to obtain 6 kg to 7 kg. In addition, the extract may be fermented microorganisms using fungi commonly used in food fermentation process.

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Heterogeneous grass;

 10 kg of dry grass powder is added, and 10 times of purified water is added and extracted with gentle stirring for 5 hours while maintaining at 95 ° C or more.

The obtained product is centrifuged, the solid residue is discarded, the extract is left for 1 day, and the supernatant is filtered and concentrated under reduced pressure.

The concentration under reduced pressure was concentrated at 55 ° C. under reduced pressure (70-76 mmHg), and the obtained solid component was concentrated to a solid content of about 60% and 6 kg-7 kg was obtained as an extract.

The extract is aged and fermented (when fermentation fermentation is carried out for 40 days while maintaining the temperature of 30 ° C. to 40 ° C.) to obtain 6 kg to 7 kg. The extract also contains fungi commonly used in food fermentation processes. Microbial fermentation can be used.

Dark bean;

10 kg of purified water is added to 10 kg of black bean dry powder, and extracted at room temperature for 12 hours.

The obtained product is centrifuged, the solid residue is discarded, the extract is left for 1 day, and the supernatant is filtered and concentrated under reduced pressure.

The concentration under reduced pressure was concentrated at 55 ° C. under reduced pressure (70-76 mmHg), and the obtained solid component was concentrated to a solid content of about 40%, and 7 kg-8 kg was obtained as an extract. This extract is aged and fermented (when fermentation fermentation is carried out for 40 days while maintaining the temperature of 30 ~ 40 ℃) to obtain 7kg ~ 8kg. In addition, the extract may be fermented microorganisms using fungi commonly used in food fermentation process.

Charcoal;

10 kg of dried water was added to 10 kg of dried powder of chajeoncho, and extracted with gentle stirring for 5 hours while maintaining 95 ° C or more.

The obtained product is centrifuged, the solid residue is discarded, the extract is left for 1 day, and the supernatant is filtered and concentrated under reduced pressure. The concentration under reduced pressure was concentrated at 55 ° C. under reduced pressure (70-76 mmHg), and the obtained solid component was concentrated to a solid content of about 60% and 4 kg to 5 kg were obtained as an extract. This extract is aged by fermentation (fermentation fermentation is carried out for 40 days while maintaining the temperature of 30 ℃ ~ 40 ℃) to obtain 4kg ~ 5kg. In addition, the extract may be fermented microorganisms using fungi commonly used in food fermentation process.

Yonggyu is;

To 10 kg of dry silica powder, add 10 times of purified water and extract with gentle stirring for 5 hours while maintaining 95 ℃ or more.

The obtained product is centrifuged, the solid residue is discarded, the extract is left for 1 day, and the supernatant is filtered and concentrated under reduced pressure. The concentration under reduced pressure was concentrated at 55 ° C. under reduced pressure (70-76 mmHg), and the obtained solid component was concentrated to a solid content of about 55%, and then 5 kg to 6 kg was obtained as an extract. This extract is aged by fermentation (fermentation fermentation is carried out for 40 days while maintaining the temperature of 30 ℃ ~ 40 ℃) to obtain 5kg ~ 6kg. In addition, the extract may be fermented microorganisms using fungi commonly used in food fermentation process.
Brown root;

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10 kg of dried dry powder is added, and 10 times of purified water is extracted.

The obtained product is centrifuged, the solid residue is discarded, the extract is left for 1 day, and the supernatant is filtered and concentrated under reduced pressure.

The concentration under reduced pressure was concentrated at 55 ° C. under reduced pressure (70-76 mmHg), and the obtained solid component was concentrated to a solid content of about 60% and 4 kg to 5 kg were obtained as an extract. This extract is aged by fermentation (fermentation fermentation is carried out for 40 days while maintaining the temperature of 30 ℃ ~ 40 ℃) to obtain 4kg ~ 5kg. In addition, the extract may be microbial fermentation using fungi commonly used in food fermentation process.

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Honeysuckle;

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10 kg of dried honeysuckle powder is added, and 10 times of purified water is extracted.

The obtained product is centrifuged, the solid residue is discarded, the extract is left for 1 day, and the supernatant is filtered and concentrated under reduced pressure.

The concentration under reduced pressure was concentrated at 55 ° C. under reduced pressure (70-76 mmHg), and the obtained solid component was concentrated to a solid content of about 60% and 6 kg-7 kg was obtained as an extract. The extract is aged and fermented (when fermentation fermentation is carried out for 40 days while maintaining the temperature of 30 ° C. to 40 ° C.) to obtain 6 kg to 7 kg. In addition, the extract may be fermented microorganisms using fungi commonly used in food fermentation process.

Licorice is;

10 kg of low alcohol (40% ethanol) of C _1-4 was added to 10 kg of licorice dry powder, and primary extraction was carried out while stirring slowly for 5 hours while maintaining at 70 ° C. or higher. Add and extract secondary at 5 hours.

The products obtained in the primary and secondary are centrifuged, the solid residue is discarded, the extract is left for 1 day, the supernatant is filtered and concentrated under reduced pressure.

The concentration under reduced pressure was concentrated at 55 ° C. under reduced pressure (70-76 mmHg), and the obtained solid component was concentrated to a solid content of about 60% and 5 kg to 6 kg was obtained as an extract. This extract is aged by fermentation (fermentation fermentation is carried out for 40 days while maintaining the temperature of 30 ℃ ~ 40 ℃) to obtain 5kg ~ 6kg. In addition, the extract may be fermented microorganisms using fungi commonly used in food fermentation process.

Gardenia;

10 kg of lower alcohol (40% ethanol) of C _1-4 was added to 10 kg of Gardenia dried powder, and extracted with 25 hours of stirring at 25 ° C.

The obtained product is centrifuged, the solid residue is discarded, the extract is left for 1 day, and the supernatant is filtered and concentrated under reduced pressure.

The concentration under reduced pressure was concentrated at 55 ° C. under reduced pressure (70-76 mmHg), and the obtained solid component was concentrated to a solid content of about 60% and 6 kg-7 kg was obtained as an extract. The extract is aged and fermented (when fermentation fermentation is carried out for 40 days while maintaining the temperature of 30 ° C. to 40 ° C.) to obtain 6 kg to 7 kg. In addition, the extract may be fermented microorganisms using fungi commonly used in food fermentation process.

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Schisandra;

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10 kg of dried powder of Schizandra chinensis shall be added with 10 times of purified water and extracted with gentle stirring for 5 hours while maintaining 95 ℃ or more.

The obtained product was centrifuged, the solid residue was discarded, the extract was left for 1 day, and the supernatant was filtered and concentrated under reduced pressure. The concentration under reduced pressure is concentrated at 55 ° C. under reduced pressure (70-76 mmHg), and the obtained solid component is concentrated to a solid content of about 60%, and then 5 kg to 6 kg are obtained as an extract.

This extract is aged by fermentation (fermentation fermentation is carried out for 40 days while maintaining the temperature of 30 ℃ ~ 40 ℃) to obtain 5kg ~ 6kg. In addition, the extract may be fermented microorganisms using fungi commonly used in food fermentation process.
On the other hand, in the present invention, one or more selected from the herbal medicines such as stomach medicinal herb, pesticide, civil engineering, Yugeunpi, Hyunho-color, pulse movement can be added, and the active ingredient extraction method of the herbal medicine is as follows.
Toothworm;
10 kg of purified water was added to 10 kg of Tofu dry powder, and extracted with gentle stirring for 5 hours while maintaining 95 ° C or more.
The obtained product is centrifuged, the solid residue is discarded, the extract is left for 1 day, and the supernatant is filtered and concentrated under reduced pressure.
The concentration under reduced pressure was concentrated at 55 ° C. under reduced pressure (70-76 mmHg), and the obtained solid component was concentrated to a solid content of about 60% and 4 kg to 5 kg were obtained as an extract. This extract is aged by fermentation (fermentation fermentation is carried out for 40 days while maintaining the temperature of 30 ℃ ~ 40 ℃) to obtain 4kg ~ 5kg. In addition, the extract may be fermented microorganisms using fungi commonly used in food fermentation process.

Tobok-ryeong;
10 kg of purified water was added to 10 kg of Tobokyeong dry powder, and extracted with gentle stirring for 5 hours while maintaining 95 ° C or more.
The obtained product is centrifuged, the solid residue is discarded, the extract is left for 1 day, and the supernatant is filtered and concentrated under reduced pressure.
The concentration under reduced pressure was concentrated at 55 ° C. under reduced pressure (70-76 mmHg), and the obtained solid component was concentrated to a solid content of about 60% and 4 kg to 5 kg were obtained as an extract. This extract is aged by fermentation (fermentation fermentation is carried out for 40 days while maintaining the temperature of 30 ℃ ~ 40 ℃) to obtain 4kg ~ 5kg. In addition, the extract may be fermented microorganisms using fungi commonly used in food fermentation process.
The potion is;
To 10 kg of powdered dry powder, add 10 times of purified water and extract with gentle stirring for 5 hours while maintaining 95 ℃ or more.
The obtained product is centrifuged, the solid residue is discarded, the extract is left for 1 day, and the supernatant is filtered and concentrated under reduced pressure.
The concentration under reduced pressure was concentrated at 55 ° C. under reduced pressure (70-76 mmHg), and the obtained solid component was concentrated to a solid content of about 60% and 4 kg to 5 kg were obtained as an extract. This extract is aged by fermentation (fermentation fermentation is carried out for 40 days while maintaining the temperature of 30 ℃ ~ 40 ℃) to obtain 4kg ~ 5kg. In addition, the extract may be fermented microorganisms using fungi commonly used in food fermentation process.
Root skin;
10 kg of purified water was added to 10 kg of dried root powder, and extracted with gentle stirring for 5 hours while maintaining 95 ° C or more.
The obtained product is centrifuged, the solid residue is discarded, the extract is left for 1 day, and the supernatant is filtered and concentrated under reduced pressure.
The concentration under reduced pressure was concentrated at 55 ° C. under reduced pressure (70-76 mmHg), and the obtained solid component was concentrated to a solid content of about 60% and 4 kg to 5 kg were obtained as an extract. This extract is aged by fermentation (fermentation fermentation is carried out for 40 days while maintaining the temperature of 30 ℃ ~ 40 ℃) to obtain 4kg ~ 5kg. In addition, the extract may be fermented microorganisms using fungi commonly used in food fermentation process.

Cormorant color;
10 kg of low alcohol (40% ethanol) of C _1-4 was added to 10 kg of the coarse orange dry powder, and primary extraction was carried out while stirring slowly for 5 hours while maintaining at 70 ° C. or higher. Add and extract secondary at 5 hours.
The products obtained in the primary and secondary are centrifuged, the solid residue is discarded, the extract is left for 1 day, the supernatant is filtered and concentrated under reduced pressure.
The concentration under reduced pressure was concentrated at 55 ° C. under reduced pressure (70-76 mmHg), and the obtained solid component was concentrated to a solid content of about 60% and 4 kg to 5 kg were obtained as an extract. The extract is aged for fermentation (fermentation fermentation is carried out for 40 days while maintaining the temperature of 30 ° C. to 40 ° C.) to obtain 4 kg to 5 kg. Microbial fermentation can be used.
Pulmonary sinus;
Add 10 times purified water to 10 kg of dried powder powder and extract with gentle stirring for 5 hours while maintaining 95 ℃ or more.
The obtained product is centrifuged, the solid residue is discarded, the extract is left for 1 day, and the supernatant is filtered and concentrated under reduced pressure.
The concentration under reduced pressure is concentrated at 55 ° C. under reduced pressure (70-76 mmHg), and the obtained solid component is concentrated to a solid content of about 60%, and then 5 kg to 6 kg are obtained as an extract.
This extract is aged by fermentation (fermentation fermentation is carried out for 40 days while maintaining the temperature of 30 ℃ ~ 40 ℃) to obtain 5kg ~ 6kg. In addition, the extract may be fermented microorganisms using fungi commonly used in food fermentation process.
When the extract is extracted from each natural herbal medicine, the solvent mixed with the dry powder of each natural herbal medicine may be purified water or lower alcohol of C _1-4 as described above, but the solvent may be purified water and lower C _1-4. It can be used as a mixed solvent of alcohol.
Next, in order to obtain the extract of the natural herbal medicine, each component may be used simultaneously or separately in an excess amount of an aqueous solvent (for example, water alone or a hydrophilic organic solvent, for example, a mixture of methanol and ethanol, ethyl acetete), that is, 5 to 20 After mixing with the amount of the pear, and distilled mixture is extracted for about 3 to 10 hours at 40 ℃ ~ 95 ℃ for a sufficient time to remove the solids, the extract is concentrated under reduced pressure, and then microbial fermentation and aging fermentation using microorganisms.
In addition, the above three hundred seconds, yirum grass, black bean, chajeoncho, yonggyu, brown root, honeysuckle, licorice, gardenia, Schisandra chinensis extract by mixing each one by weight to obtain the main herbal composition, or to the main herbal composition to worms, powdered soil Freeze-dried lyophilized one or more extracts selected from Fukryeong, Yu-Geun Skin and Hyun-ho-Sang pulse exercise may be added.
What is described below measured the efficacy of the present invention as an experimental example of the herbal composition obtained through the present invention.

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Experimental Example 1

Determination of Nicotine Degradation in Blood by Animal Clinical Trials

1) Test Animal

Wister strain male rats (6 weeks old) are purchased from Samtaco (Osan-si, Gyeonggi-do).

2) Test environment

Animals were housed in 5 wire cages in an animal room set at room temperature 22 ± 1 ° C., humidity 55 ± 5%, ventilation time 12 times / hour, lighting time 12 hours / day (light at 8 am, light at 8 pm). . Forage was fed solid water (Sam # 31 (Autoclavable)) with water.

3) Administration method

Adapted to the test environment for 2 weeks and animals were used at 8 weeks of age.

The test substance was mixed with 0.4 g of the composition and 3.6 g of solid feed, and total 4 g was orally administered to the body as free food with water.

4) Dosing time

Administration was performed at 2 pm and the period was 7 days.

At the end of dosing and at the end of the test, 1 ml of blood was drawn from the heart as a sample for nicotine resolution measurement.

5) Observation Method

Dosing days were observed immediately after administration and in the evening, and the morning and evening thereafter. Observation was done carefully from outside the breeder

6) Test result of nicotine resolution in blood

Blood was adjusted to pH 9 with dilute ammonia water. Subsequently, the resultant was kept in EXTRELUT COLUMN. (Manufactured by Merck Co., Ltd.) for 15 minutes, eluted with 15 ml of ethyl acetate, the eluted acetate was distilled off under reduced pressure, and 100 µl of ethyl acetate was added to the resulting residue to dissolve.

For analysis, use gas chromatography (HP 5890 SERIESП with FID).

COLUMN used a glass column 2 m long and 3 mm in diameter filled with 2% Tharmon 1000 + 1% KOH Chromosorb WAM (8 / 100Mesh).

COLUMN temperature was 100 ℃, N ₂ was used as the carrier gas and the flow rate was 60ml / min.

Table 1. Results after 7 days administration of the composition

paralysis Symptom Hyperactivity Start time Hyperactivity End time Death time In the blood Nicotine Concentration (µg / ml) G 1 One 27 " 2'10 " 5'50 " recovery 0.024 2 36 " 2'44 " 6'10 " recovery 0.027 3 43 " 3'25 " 6'51 " recovery 0.015 4 43 " 3'50 " 5'56 " recovery 0.021 5 33 " 3'52 " 6'35 " recovery 0.017 G 2 One 22 " 4'42 " 6'45 " 31'18 " 1.037 2 26 " 4'46 " 6'35 " recovery 0.014 3 20 " 5'40 " 7'25 " recovery 0.012 4 24 " 5'47 " 7'20 " recovery 0.013 5 23 " 4'46 " 6'47 " recovery 0.019 G 3 One 23 " 35 " 56 " 1'33 " 12.178 2 33 " 45 " 1'15 " 1'35 " 12.183 3 29 " 31 " 1'20 " 1'50 " 12.987 4 25 " 54 " 1'35 " 1'57 " 12.969 5 22 " 51 " 1'50 " 2'10 " 11.719 G 4 One 33 " 2'50 " 4'20 " 6'15 " 4.019 2 25 " 2'40 " 4'50 " 7'18 " 3.679 3 24 " 2'45 " 4'47 " 6'45 " 4.095 4 21 " 2'37 " 5'10 " 6'25 " 4.074 5 33 " 2'35 " 4'15 " 6'54 " 4.117

Note: G1: Nicotine administration after pretreatment of the composition.

G2: Anti-drug administration after Nicotine administration after pretreatment of this composition.

G3: control Nicotine.

G4: Administration of the invention after administration of control Nicotine.

Ten test subjects were tested in each group.

Nicotine concentration 40mg / kg / weight ratio (dose ml)

Experimental Example 2

Determination of Nicotine Degradation in Urine by Animal Clinical Experiment

1) Test Animal

Wister strain male rats (6 weeks old) are purchased from Samtaco (Osan-si, Gyeonggi-do).

2) Test environment

Five animals were housed in a wire cage in an animal room set at room temperature 22 ± 1 ° C., humidity 55 ± 5%, ventilation number 12 times / hour, lighting time 12 hours / day (light at 8 am, light at 8 pm). . Forage was fed solid water (Sam # 31 (Autoclavable)) with water.

3) Administration method

Adapted to the test environment for 2 weeks and animals were used at 8 weeks of age.

The test substance was mixed with 0.4 g of the composition and 3.6 g of solid feed, and total 4 g was orally administered to the body as free food with water.

4) Dosing time

Administration was performed at 2 pm and the period was 7 days.

    At the end of dosing and at the end of the test, 1 ml of blood was collected from the bladder as a sample for measuring nicotine resolution.

5) Observation Method

Dosing days were observed immediately after administration and in the evening, and the morning and evening thereafter. Observation was done carefully from outside the breeder

6) Test result of nicotine resolution in urine

Urine was adjusted to pH 9 with dilute ammonia water. Then EXTRELUT COLUMN. (Merck Co., Ltd.) was maintained for 15 minutes, eluted with 15 ml of ethyl acetate, the eluted acetate was distilled off under reduced pressure, and 100 µl of ethyl acetate was added to the resulting residue to dissolve and used as an assay sample.

For analysis, use gas chromatography (HP 5890 SERIESП with FID).

COLUMN was filled with 2% Tharmon 1000 + 1% KOH Chromosorb WAM (8 / 100Mesh) and used a glass column with a length of 2 m and a diameter of 3 mm.

The COLUMN temperature was 100 ° C, the carrier gas was N ₂ , and the flow rate was 60 ml / min.

Table 2. Results after 7 days administration of the composition

paralysis Symptom Hyperactivity Start time Hyperactivity End time Death time Urinary Nicotine Concentration (µg / ml) G 1 One 22 " 2'20 " 5'30 " recovery 5.524 2 34 " 2'34 " 6'30 " recovery 5.527 3 43 " 3'45 " 6'31 " recovery 5.515 4 46 " 3'40 " 5'36 " recovery 5.531 5 30 " 3'32 " 6'15 " recovery 5.517 G 2 One 23 " 4'32 " 6'35 " recovery 5.537 2 23 " 4'36 " 6'55 " recovery 5.514 3 34 " 5'50 " 7'55 " recovery 5.512 4 21 " 5'17 " 7'40 " recovery 5.513 5 20 " 4'26 " 6'37 " recovery 5.519 G 3 One 20 " 30 " 57 " 1'43 " 0.021 2 33 " 55 " 1'35 " 1'55 " 0.031 3 23 " 41 " 1'50 " 2'30 " 0.037 4 24 " 54 " 1'35 " 1'57 " 0.021 5 25 " 55 " 1'55 " 2'30 " 0.029 G 4 One 31 " 2'30 " 4'10 " 6'45 " 0.219 2 23 " 2'30 " 4'45 " 7'25 " 0.279 3 23 " 2'55 " 4'37 " 6'15 " 0.215 4 23 " 2'47 " 5'50 " 6'55 " 0.214 5 30 " 2'55 " 4'45 " 6'14 " 0.217

  Note: G1: Nicotine administration after pretreatment of the composition.

G2: Anti-drug administration after Nicotine administration after pretreatment of this composition.

G3: control Nicotine.

             G4: Administration of the invention after administration of control Nicotine.

Ten test subjects were tested in each group.

Nicotine concentration 40mg / kg / weight ratio (dose ml)

Experimental Example 3

Determination of Carbon Monoxide Concentration in Blood by Animal Clinical Experiment

1) Test Animal

Wister strain male rats (6 weeks old) are purchased from Samtaco (Osan-si, Gyeonggi-do).

2) Test environment

Animals were housed in 5 wire cages in an animal room set at room temperature 22 ± 1 ° C., humidity 55 ± 5%, ventilation time 12 times / hour, lighting time 12 hours / day (light at 8 am, light at 8 pm). . Forage was fed solid water (Sam # 31 (Autoclavable)) with water.

3) Administration method

Adapted to the test environment for 2 weeks and animals were used at 8 weeks of age.

The test substance was mixed with 0.4 g of the composition and 3.6 g of solid feed, and total 4 g was orally administered to the body as free food with water.

4) C0 expose method

The day after the end of the administration, each animal was housed in an exposure chamber (manufactured by Sugiyamage, Japan), and a mixed gas of 21% O ₂ and 100 ppm CO was introduced into the pump and aspirated for 5 minutes. The concentration in the chapter was examined with a carbon monoxide meter (COM-4, manufactured by Komei Rikagaku Kogyo, Japan).

5) Measurement of CO-Hb Concentration in Blood

Immediately after the end of the exposure, blood was drawn from the heart, 0.5 ml of blood was added to a 5 ml volume of reaction vial, and one drop of octyl alcohol and 0.25 ml of saturated aqueous potassium ferricate solution were added. After the reaction solution was capped and shaken for 5 minutes, gas phase was used as analytical sample.

For analysis, use gas chromatography (HP 5890 SERIESП, TCD).

COLUMN used a glass column 2m in length and 3mm in diameter filled with molecular sieve 5A (3 / 60Mesh).

The COLUMN temperature was 60 ° C., the carrier gas was H ₂ , and the flow rate was 50 ml / min.

6) Results

The results of measuring the CO concentration in the blood after the test are shown in Table 3.

      Table 3. Results after 10 days administration of this composition

CO-Hb concentration in blood (%) G 1 One 53.8 2 52.9 3 50.3 4 52.8 5 53.5 G 2 One 53.0 2 52.8 3 53.5 4 52.9 5 53.0 G 3 One 69.3 2 70.2 3 69.1 4 68.5 5 68.9 G 4 One 61.5 2 60.8 3 62.9 4 62.1 5 61.8

Experimental Example 4

Antioxidative effect

Free radicals are atherosclerosis (Palinski, W., Rosenfeld, ME, Yla, HS, Gurtner, GC, Socher, SS, Butler, SW, Carew, TE, Steinberg, D., and Witztum, JL (1989) Proc . Natl . Acad. Sci., 86, 1372-1376), ischemia by vasoconstriction (Hammond, B., Kontos, HA , and Hess, ML (1985) Can. J. Physiol. Pharmacol., 63, 173-187 ), Inflammation (Cheeseman, KH, and Forni, LG (1988) Biochem . Pharmacol ., 37, 4225-4233), carcinogenesis (Weitzman, SA, Weitberg, AB, Clark, EP, and Stossel, TP (1985) Science , 227, 1231-1233), rheumatoid arthritis (Fantone, JC, and Ward, PA (1985) Human Pathol . , 16, 973-978). Various evidences have been known to mediate a wide range of diseases. Therefore, free radical scavenger is expected to be an effective treatment by reducing free radical levels, and people have focused on developing safe and effective antioxidants.

In this experiment, a method for measuring antioxidant effects by free radicals DPPH (1,1-diphenyl-2-picrylhydrazyl) and Flow-Injection Chemiluminescence (FI-CL) (Analytical Biochemistry 264, 291-293) SOD (superoxide dismutase) functional agent Was tested against.

1) Method of measuring antioxidant effect by DPPH (1,1-diphenyl-2-picryl-hydrazyl)

Dissolve the stable free radical DPPH in ethanol to make 0.5 mM solution, add 1 ml of 100 mM Tris-HCl buffer (pH 7.4) to 1 ml of DPPH solution, and add 0.1 ml of 0.5% test sample to 15 ℃ in the dark. After the reaction, the absorbance was measured at 517 nm. Absorbance was measured using an HP UV / VIS spectrophotometer. The antioxidant effect of each test sample was compared with the UV absorbance of the control without DPPH but no sample and the absorbance of the test tube with 0.5% sample in DPPH. The radical scavenging activity of the composition was expressed as% inhibition.

% Inhibition = [A-B] / [A] x 100

A: absorbance only DPPH

B: Sample absorbance mixed with DPPH + sample (scavenger)

The composition vitamin C (control) Average 92.47 79.80 Standard error 0.02 1.30

2) Method of measuring antioxidant effect by Flow-Injection Chemiluminescence (FI-CL) (Analytical Biochemistry 264, 291-293)

The chemiluminescence detector can be used to determine the antioxidant effect by removing reactive oxygen species (ROS) generated by hydrogen peroxide. CL intensity was measured by connecting a pump (Gilson Model 306) and an injector (Rheodyne Model 7125) to a spectrophotometer (CLA-1100, Tohoku Electronic Industry), a filter equipped photon counting type. Chemiluminescence is the reaction of cytochrome c with H ₂ O ₂ to produce oxygen-derived free radicals, and the product reacts with luminol to cause oxidation of luminol, resulting in chemiluminescence. At this time, free radicals are reduced by antioxidants, which act as radical scavenger, resulting in a decrease in CL intensity. The mobile phase was prepared by adding cytochrome c (10 mg / L) and luminol (2 mg / L) to 50 mM phosphate buffer (pH 7.4) made of 50% methanol. The flow rate was 1 ml / min. 5 μl of 0.06% H ₂ O _{2 and} 5 μl of 0.1% test sample were injected through the injector. Antioxidant effect of the test sample is compared to the decrease in CL intensity appeared to injection with a 0.1% sample in 0.06% H ₂ O ₂ in a CL intensity as a control, and 0.06% H ₂ O ₂ are shown by the radical scavenging activity.

% radical scavenging activity = [A-B] / [A] x 100

A: Chemiluminescence generated after injection of H ₂ O ₂ only

B: Chemiluminescence generated after injection of H ₂ O ₂ + sample (scavenger)

The composition vitamin C Average 98 100 Standard error 0.01 0.00

Experimental Example 5

Nitrite scavenging effect

The functional preparation of the present invention was tested to inhibit the formation of carcinogenic nitrosamine by-products.

The nitrite scavenging action reduces the activity of the precursors of the nitrosamines, which are carcinogenic substances, to investigate the inhibitory function of nitrosamines and to measure the inhibitory effect of nitrosamines production in vivo by the extract of the present invention.

This method creates a Nitrosation model system: morpholin and NaNO ₂ While reacting the solution, the Nitrosation inhibition effect by the extract of the composition is measured.

Inhibition of Nitrosation In this Response The measurement of the inhibitory effect in the nitrosation reaction can effectively suppress the occurrence of cancer, one of the major diseases in modern society, by searching for the inhibition of the production of various nitrosamines synthesized in the stomach of humans or animals. Not only can you get information about the possibilities, but you can also check the various functionality.

After spreading the composition (1.5%) evenly on each cigar, smoke dried cigarettes to collect nitrite scavenging ability was Kato experiment (Kato, H., Lee, IE, Chuyen, NV, KIM, SB and Hayase, F.Inhibition of nitrosamine formation by nondialyable melanoidins.Agri.Bio.Cham. 51 (5): 1333-1338 (1987). and Kytopoulos, SA Ascorbic acid and formation of N-nitroso compounds; Possible role of ascorbic acid in cancer prevention.Am. J. Clin. Nutr. 45: Measured according to the method of 1344-1350 (1987), ie, 1 mL of each of the compositions was added to ₂ mL of 1 mM NaNO ₂ solution and 0.1 N HCl (pH 1.2) and 0.2 M citrate buffer (pH 4.2). ), The pH of the reaction solution was adjusted to 1.2 and 4.2, respectively, so that the total volume was 10 mL, and the reaction was carried out for 1 hour at 37 ° C. 1 mL of the reaction solution was taken, and 5 mL of 2% actic acid and griess reagent were added thereto. After adding 0.4mL, the solution was blocked at room temperature for 15 minutes and the absorbance was measured at 520nm. Griess reagent quantified 1% equivalents of sulfanilic acid (Sigma, co.) And naphthylamine (Sigma, co.), Respectively, and added 100% acetic acid to stir and dissolve it. The nitrite scavenging rate was expressed as the percentage of absorbance with and without sample solution, and vitamin C 10mg / 100g was used as a control.

      SA (%) = (1-A-B / B) X 100

      SA: Nitrite scavenging ability

A: Absorbance of 1mM NaNO ₂ added sample after standing for 1 hour

B: Absorbance of NaNO ₂

      C: Absorbance of control

Experimental results are the results of examining the nitrite scavenging rate for the functional product of the composition is shown in Table 4. The functional product for this composition showed the highest scavenging rate as a whole at 55.75 ~ 99.86% under the condition of pH 1.2, and the scavenging rate was significantly decreased as the pH was increased to 10.25 ~ 34.22% at pH 4.2. The optimum pH for nitrosamine production was 2.5 ~ 3.0, which showed the same tendency to decrease with increasing pH.

Since nitrosamine is easily formed in the gastrointestinal (low pH) or tobacco smoke, the high nitrite scavenging rate at low pH seems to effectively inhibit nitrosamine formation. The nitrite scavenging rate of vitamine C and 10 mg used as a control group was 98.93% at pH 1.2 and 92.91% at pH 4.2.

Table 4

pH 1.2 pH 4.2 Vit.c 98.93 Vit.c 92.91 The composition 99.86 The composition 34.22

Experimental Example 6

Splenocyte proliferation effect by the present composition

NO is a new bioregulator that is recently involved in pharmacological and toxic effects. Brain toxicity, of course, acts as a target to neutralize the intrinsic action of myocytes, hepatocytes, neurons. However, when NO reacts with other species with unpaired electrons, it also produces a more toxic secondary, which is a direct cause of fatal tissue damage.

In general, macrophages have three major antimicrobial activities.

First is the increase in various hydrolytic enzymes contained in intracellular lysosomes.

Second is the generation of ROI by NADH oxidase activity,

Third is the production of NO.

Excess ROI and NO, however, damage tissues and cause inflammation. Therefore, the production of these two active substances needs to be regulated, and studies have shown that NO and ROI are produced simultaneously or promoted by either.

In the test of the present invention, RAE264.7, a mouse macrophage, was collected at 1 × 10 ⁵ cells per well, and cultured for 2 hours in a 37 ° C. CO ₂ incubator to remove non-attached dead cells. (100 ng / ml) was mixed and treated and incubated for 24 hours. After completion of the culture, nitrite (a stable aqueous compound of NO) in the culture supernatant was quantified using Griess reagent using NaNO ₂ as a standard.

Table 5. Inhibition effect of L on NO production in RAW 264.7 (Each value represents mean ± S.D. of triplicates.

Dose (µg / ml) Not LPS + LPS (100 ng / ml) Viability (%) 0 3.3 ± 1.2 25.4 ± 4.3 100 0.5 3.2 ± 1.2 23.5 ± 3.3 97 5.0 4.7 ± 1.6 22.6 ± 5.8 98 50.0 5.6 ± 2.1 21.9 ± 4.8 96 500.0 4.9 ± 2.1 10.2 ± 3.6 95

Experimental results show that the composition is not toxic to animal cells (experimental to 1 mg / ml), as shown in the table above, weaker than mitogen (LPS. ConA), but promotes the proliferation of mouse immune cells (splenocytes). Although weaker than NO inhibitors (NMMA, Polymicin B), they inhibit NO production in RAW (inhibition of Inos or direct reduction of NO). In vitro experiments induce the activation of immune cells and increase the immune activity in vivo. have.

Experimental Example 7

AMES TEST

Tobacco smoke contains about 4,000 toxic substances. In order to find out how much to reduce the toxicity of this toxic substance, the experiment was conducted by the following test method (AMES TEST).

To assess antimutagenic induction, mutations are induced by B (a) P and the inhibitory action by the extract is verified. In the model system, mutagencity is added by using concentrations of Aflation B ₁ , benzo (α) Pyrene (B (a) P), and some Nitrosamines, and smoke condensate is added to each concentration. To investigate.

☞ Purpose: Ames test using Salmonella bacteria is a method to check whether it shows mutagenic activity during metabolism by microsomal mixid function oxidase system that plays a major role in metabolism. Investigate the inhibition effect by extract.

Table 6. Results of the AMES Test of this composition (X ± SD).

Composition (mg / plate) TA ₉₇ TA ₉₈ TA ₁₀₀ TA ₁₀₂ -S ₉ + S -S ₉ + S -S ₉ + S -S ₉ + S One 15 144 32 41 10 12 31 30 5 9 163 38 39 5 4 8 0 25 15 113 34 36 11 11 32 27 120 0 168 35 36 One 6 0 9 Control 12 147 39 36 12 11 31 31 9 5 3 5 2 12 16 12 31 30 9 4 4 7 5 12 11 10 27 31 7 9 8 8 0

Experimental results It is the result of experiment by the test method (AMES TEST) to find out how much toxic substances are reduced in the tobacco smoke.

According to the concentration of smoke condensate, we tested the effects of cigarette smoke reduction on nitrosamines that cause mutagenic activity and toxic substances of tobacco smoke, especially carcinogenic cancers. It was shown to be mitigating.

Experimental Example 8

Bone marrow nuclear test

Chromosomal damage was examined by the method of mouse bone marrow micronucleus test of the composition.

Table 7. Mouse bone marrow nucleus test result

  dose Macronucleus number Macronucleus ratio P female cock female cock Control - 40 36 8.0 7.2 - positive 40mg / kg 160 120 32.6 24.4 <0.0 The composition 5g / kg 41 32 8.2 6.4 One The composition 10g / kg 37 30 7.4 6.0 5 The composition 20g / kg 41 29 8.2 5.8 〉 0.0 5 〉 0.0 5

The results of the macronucleus test of male and female bone marrow micronucleus cells showed that the chromosomal aberrations of the myeloid nucleus cells of the mouse were not observed after 5-20 g / kg of the composition was administered in a single dose range.

Experimental Example 9

Small Rat Spermatology Test

The small rat sperm malformation test of the present composition was examined to determine whether sperm count was damaged.

Table 8. Small mouse sperm malformation test

dose Animal number Observed sperms Abnormality sperms Abnormality Ratio (%) Control - 5 5000 52 1.04 Positive 60mg / kg 5 5000 250 5.00 The composition 5g / kg 5 5000 50 1.00 The composition 10g / kg 5 5000 52 1.04 The composition 20g / kg 5 5000 69 1.38

The test results showed that there was no abnormal change in mouse sperm count when the composition was administered in a dose of 5-20 g / kg.

Experimental Example 10

2-Aminofluorene (2-AF) Test

2-AF is the strongest chemical carcinogen known in the world. Inhibition of 2-aminofluorene is as follows.

Table 9. Influence of the invention on mutagenicity of 2-Aminofluorene (2-AF).

Main composition (mg / plate) 2-AF (μg / plate) TA ₉₇ (+ S ₉ ) TA ₉₈ (+ S ₉ ) TA ₁₀₀ (+ S ₉ ) 0 10 1518.0 ± 92.2 > 2000 674.0 ± 38.5 15 10 338.1 ± 1.46 179.3 ± 4.5 342.7 ± 15.5 30 10 190.3 ± 2.74 46.0 ± 3.6 132.0 ± 27.6 60 10 161.3 ± 5.6 35.7 ± 4.2 128.7 ± 9.3 120 10 141.3 ± 5.6 29.0 ± 1.0 112.3 ± 13.6 Control - 144.7 ± 5.8 28.7 ± 0.6 117.3 ± 4.2 DMSO - 141.3 ± 5.6 30.3 ± 2.1 117.3 ± 5.5

As a result, the positive purpose of activating 2-AF was to cause mutation, and the composition was able to significantly inhibit the mutagenicity of 2-AF. And at a single dose of 120 mg / plate, the composition was able to completely eliminate the mutagenic effects of 2-AF.

Experimental Example 11

NNN in TAR of tobacco. NAT. NNK. B (a) P Analysis

The atmosphere contains hundreds of chemicals, along with smoke from automobile exhaust, factory chimneys, and heating in houses and buildings. Among them, benzopyrene is known as a substance causing lung cancer, and in particular, a problem due to smoking has been raised.

The faster metabolism of nicotine from smoking than the conversion of nicotine into the nicotine derivatives NNK, NNN and NAT can reduce the damage caused by smoking and ultimately inhibit carcinogens by reducing Benzo (a) pyren. .

It is the result of measuring tobacco smoke by adding this composition to tobacco.

1. Reagent

  1.1 Standard

NNN, NAT, NNK, and B (a) P (2000 µg / ml, Supelco, USA) were used diluted to a concentration range of about 1.0 to 40.0 ng / ml.

2. Appliances and Devices

2.1 Gas Chromatography
The gas chromatography used was Hewlett Packard (USA) 6890 Plus Detector used Flame Ionized Detector, Mass Selective Detector, HP 5973.

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2.2 Automatic Smoking Device
Automatic smoking device is Heinr. A 20-port smoker from Borgwaldt (Germany) was used.

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3. Analysis method

   3.1. Test cigarette

3.1.1 SAMPLE cigarette manufactured by adding this composition in Tianjin tobacco production window,

3.1.2 HANDA Tobacco Manufactured at Tianjin Tobacco Manufacturing Window, China

3.2. Cigarette production

2 ml of distilled water was added to 100 mg of the present composition, and the present invention was dissolved well, evenly sprayed onto 20 g of tobacco leaves, and dried at 50 ° C. for 24 hours.

3.3. NNN of Tar in Tobacco. NAT. NNK. Benzo (a) pyrene) Assay

3.3.1 Combustion conditions

Each test cigarette was prepared and burned to CORESTA standard conditions. Combustion conditions were 35 ± 0.5ml of smoking volume, 2.0 seconds of smoking time and 60 seconds of smoking cycle. The length of the butts was adjusted to the length of the tip paper with filter +3 mm.

The smoking profile was designed to maintain a bell shape that meets ISO 3308.

3.2.2 N'-nitrosonor nicotine in tar

N'-nitrosoanatabine (NAT).

NNK (4- (methylnitrosamino) -1- (3-pyridyl) -1-butanone).

Quantitative analysis of Benzo (a) pyrene (B (a) P).

Table 10. Quantitative Analysis Results (Unit: ng / bare)

NNN NAT NNK B (a) P SAMPLE 0.86 ± 0.12 1.11 ± 0.18 0.45 ± 0.12 5.78 ± 0.31 CONTROL 2.43 ± 0.25 4.26 ± 0.43 1.72 ± 0.17 19.59 ± 0.54 Content difference (%) 64.61 73.94 73.84 70.50

4. Test result

Among tobacco tars, NNN, NAT, NNK, and B (a) P test resulted in an average 70.723% reduction in SAMPLE (2% additional composition) than the CONTROL group.

Experimental Example 12

Effect of this composition on proliferation of mouse splenocyte (immunity test)

Splenic cell culture Way

Spleens from three-week-old ICR mice were washed with physiological saline to separate splenocytes from complete RPMI 1640 media. The isolated splenocytes were dispensed at 96 x 2 wells at 2 x 10 ⁵ cells / ml and incubated with stimulators such as Con A and LPS, and treated with different concentrations of the composition at 37 ° C and 5% CO _2. After incubation in the incubator for two days, the absorbance was measured using a microplate reder by treating alarmar blue with 20µl / well.

* Concentration of this composition: 20, 50, 100, 200 ug / ml

* All experiments were performed in a clean bench, and all samples including media were filtered (22 μm) and treated to cells.

* micro plate reder: measured at measurment 570 nm, referance 600 nm

** Alamar Blue assay: Alamar blue dye is a non-toxic cytotoxity assay that uses metabolic properties of cells to turn blue. When the OD value measured by the micro plate reder is low, the cell proliferation is estimated to be reduced.

The test results are as follows.

Two days of incubation with Con A as stimulus, followed by alamar blue.

Table 10. It is the result about this composition of the state which passed 32 hours.

Average Standard error normal -0.05 0.01 control 0.09 0.00 Main composition 200 0.14 0.01 Main composition 100 0.13 0.01 Main composition 50 0.11 0.01 Main composition 25 0.08 0.01

Experimental Example 13

Results of Animal Testing (PLASMA)

1. Experimental Method: SD-rat at 3 weeks of age was divided into groups of Normal, Nicotin, and Nicotine + the present invention (n = 7) and sacrificed at 7 weeks of age after breeding.

Normal (n = 6): Free expression

Nicotine (n = 8): nicotine 4mg / kgBW oral administration + 200mg / kgBW physiological saline intraperitoneal administration

Nicotine + composition (n = 7): nicotine 4mg / kgBW oral administration + 200mg / kgBW this composition intraperitoneally

2. Plasma Analysis

Blood was collected from the arteries of the sacrificed animals and treated with EDTA to prevent coagulation. Collected blood was centrifuged at 3,000 rpm for 10 minutes to separate serum and stored at -70 ℃ until analysis.

 1) Total Cholesterol: Using the blood total cholesterol measurement kit (Wako, Japan), absorbance was measured and measured by UV-VIS Spectrophotometer 505nm.

 2) Triglyceride: Using a blood triglyceride measurement kit (Wako, Japan), absorbance was measured on the UV-VIS Spectrophotometer at 410 nm.

3. Determination of Lipid Peroxidation Content (TBARS)

After administration of Nicotine and this composition for 4 weeks, the liver was extracted by anesthesia with diethyl ether. The isolated liver was stored at -70 ° C until analysis.

After 1 g of liver was homogenated with 100 mM Tris-HCl, pH 7.4 buffer, TBA color reagent was added to quantitatively analyze the TBARS content generated at 535 nm.

Table 11. Experiment result

Plasma (mg / dl) Normal Nicotine Nicotine + main composition Triglyceride 37.2 ± 6.0 ^a 77.1 ± 3.8 ^b 46.3 ± 2.7 ^a Total cholesterol 89.8 ± 3.2 ^c 68.0 ± 1.9 ^b 43.7 ± 3.0 ^a Glucose 151.5 ± 2.4 ^a 174.4 ± 2.7 ^b 197.3 ± 3.5 ^c

TBARS (nmol / mg) Normal Nicotine Nicotion + main composition Liver 1.0 ± 0.0 ^b 0.91 ± 0.0 ^a 0.8 ± 0.1 ^a Brain 2.2 ± 0.1 2.0 ± 0.1 2.1 ± 0.1 Lung 1.5 ± 0.1 1.5 ± 0.1 1.5 ± 0.1 Plasma 0.3 ± 0.0 0.3 ± 0.0 0.3 ± 0.1

What is TBARS- (ThioBarbutic Acid -Recative Substance).

Determination of lipid peroxidation by measuring MDA (MalonDiAldehyde-lipid peroxidation product) reacting with TBA reagent

Experimental Example 14

Single Oral Dose Toxicity Test Using Rats of the Composition

1) Purpose: To investigate the toxicity by single oral administration in rats of this composition.

2) Test method: This test was conducted in accordance with Korean Food and Drug Administration Notification No. 1999-61 (December 22, 1999), 'Toxicity Test Standards for Drugs'.

3) Animal Breeding Room: SPF Laboratory Animal Center, Central Equipment Center

4) Storage of test substance: The test substance used in this test was refrigerated.

1. Summary

In order to investigate the toxicity by single oral administration in rats of the present composition, male and female were administered to five Sparague-daweley (SD) strain rats in excipient control and test substance doses for 2 weeks of mortality, general symptoms, weight change and Autopsy findings were observed.

(1) No dead animals were observed during the entire test period.

(2) No abnormal symptoms related to administration of test substance were observed in general symptoms.

(3) Significant body weight change between groups was not recognized in both male and female in oral administration through the entire study period.

(4) There were no toxicological abnormalities associated with the administration of test substance in autopsy findings.

(5) results

From the above test results, no unusual toxicity symptoms associated with the test substance were observed in the male and female rats of the composition under the test conditions. Therefore, the minimum lethal dose (MLD) in the rat of the composition was 2,000 mg / kg It was thought to be higher than that.

2. Compositions and Excipient Controls

(1) Test substance

 1) Name: Complex Herbal Extract

2) Quantity of water: 20g (including container)

3) Date of acquisition: February 28, 2004

4) Appearance and appearance: brown powder

5) Storage condition: Refrigerated storage

(2) excipient control substances

1) Name: sterile water for injection

2) Manufacturer: Korea Pharmaceutical Industry Co., Ltd.

3) Manufacturing Number: 04D3F21

3. Materials and Methods

(1) test system

1) Species and strains

SPF rats of Spraue-dawley (SD) strain

2) Source

Orient Co., Ltd.

Address: 699-13, Mokdong-ri, Buk-myeon, Gapyeong-gun, Gyeonggi-do

3) Reason for selection of test system

Rats used in this study are suitable for toxicity test and are widely used for general toxicity test. In addition, the rats of this system have accumulated abundant test basis data and can use these data when interpreting and evaluating the test results.

4) Age and weight range

Receipt of age: 4 weeks of age and sex

Number of Animals: 17 Male and Female

Body weight at arrival: Male (80-90g), Female (70-80g)

Age at start of administration: 5 weeks for male and female

Number of animals at start of administration: 5 male and female

5) Quarantine and Purification

Upon receipt, the animals were inspected and quarantined with reference to the pathogen test report provided by the supplier. The animals were purified in the animal room, which was tested for 7 days after the animals were obtained. General symptoms were observed during the acclimation period and only healthy animals were given to the test.

(2) Breeding environment

1) Environmental conditions

Daegu Catholic University SPE with temperature 23 ± 3 ℃, relative humidity 55 ± 15%, ventilation frequency 10-20 times / h, lighting time 12hr (08:00 to 20:00 off) and illuminance 150 ~ 300Lux Laboratory animals were performed in the GLP nursery.

2) Monitoring of breeding environment

During the test, the temperature and humidity of the animal room were measured every hour by an automatic temperature and humidity meter using a computer system, and the environmental conditions such as the number of times of ventilation and illuminance were measured regularly. No environmental abnormalities were observed during the test period.

3) Identification of breeding box, breeding density and breeding box

Animals were bred in stainless net cages (215W × 360L × 200Hmm) for 5 rats in both the periods of acclimation and observation. Breeding boxes were identified by affixing individual identification cards containing test and animal numbers.

4) Feeding method of feed and water

The feed was freely ingested with sterilized test animal solid feed (Harlan Co., Ltd., USA) supplied by Polar International.

(3) Dosage and composition of test group

In the preliminary test, the dose was set to distilled water, 2000 mg / kg, 500 mg / kg, 50 mg / kg, and 5 mg / kg. Two days of observation showed no beneficial toxicity, so this study was administered with five male and female females at 2000 mg / kg body weight.

4. Results

(1) Death animals and minimum cooking quantity

No dead animals were observed in the test group during the entire trial. Therefore, it was observed that the rat exceeded the minimum dose limit of 2000 mg / kg in rats.

(2) General symptoms (Table 12)

No symptoms were observed in all groups during the entire trial.

(3) Weight change (Table 13)

No abnormal symptoms were observed in male and female body weight changes.

(4) autopsy findings

An autopsy was not performed because there were no specificities in death, abnormal symptoms, and weight changes.

Table 12. Incidence of clinical signs in male rats after single oral administration of herbal extracts (group summary)

MALE dAY SINGE OBSERVEd DOSE LEVEL (mg / kr) 0 2000 0 Appears normal 5/5 5/5 One Appears normal 5/5 5/5 2 Appears normal 5/5 5/5 3 Appears normal 5/5 5/5 4 Appears normal 5/5 5/5 5 Appears normal 5/5 5/5 6 Appears normal 5/5 5/5 7 Appears normal 5/5 5/5 7 Appears normal 5/5 5/5 9 Appears normal 5/5 5/5 10 Appears normal 5/5 5/5 11 Appears normal 5/5 5/5 12 Appears normal 5/5 5/5 13 Appears normal 5/5 5/5 14 Appears normal 5/5 5/5

; Number of animals with the sign / Number of animals examined

Table 13. Body weight change in male rats after single oral administration of Herbal extracts (group summary)

gender number Dosing concentration (mg / kg) Body weight at administration (g) After 14 days (g) Weight change   female One 2000 186 283 97 2 2000 171 267 96 3 2000 188 300 112 4 2000 197 316 119 5 2000 193 304 111   cock One 2000 156 206 50 2 2000 148 182 34 3 2000 151 196 45 4 2000 146 202 56 5 2000 139 191 52

As confirmed in the above experiments, the composition of the present invention has an excellent effect on the decomposition and release of harmful substances in the human body. Therefore, the composition of the present invention is very useful as an antidote for nicotine poisoning or other harmful substances by tobacco smoking.

The composition of the present invention may be used as a preparation form used in conventional foods or pharmaceuticals, such as tablets, powders, liquids, syrups, capsules, injections, etc., or may be prepared by ingesting it in a drink or tea, It is very easy for someone with ordinary knowledge in this field.

The composition of the present invention may be used in addition to beverages, confectionery, animal or fish feed, oral toothpaste, soap.

The compositions of the present invention are ingredients that have been used for a long time as food or medicine, and therefore are ingredients that have been found to have little toxicity.

As is evident in the above experimental example, the composition of the present invention accelerates the decomposition of nicotine and functional agents that detoxify toxic components in the human body due to smoking, and inhibits the formation of carcinogenic nitroso-compounds and benzopyrene. In addition, it inhibits the formation of carcinogens by tobacco, exhibits antioxidant effects, inhibits mutagenic activity by NNK, NNN, NAT and benzopyrene, and suppresses lung cancer incidence.

Therefore, the composition of the present invention reduces or eliminates toxic components in the human body due to smoking, reduces damage caused by smoking, and also reduces the amount of nicotine in the blood, thereby reducing or eliminating toxic components in the human body caused by smoking. Diseases can be prevented beforehand.

Moreover, the composition of the present invention is consumed as a food or a food formulated as a functional product, so as to reduce or eliminate smoking toxic components in the human body, it is necessary to stop smoking habits, use other items or add extra actions when smoking. none.

Therefore, it is not only toxic, but it can be applied to functional food additives and tobacco additives as well as to reduce immunity and defense ability due to the widespread contamination of various harmful substances that are very problematic in modern society. Can be taken without mask.

Claims (19)

From the extract of 1 to 10 parts by weight of Tobacco, Toothpaste, and Tobok-ryong, respectively, to the common ingredients extracted from 1 to 10 parts by weight, respectively, three hundred seconds, green grass, black bean, chajeoncho, yonggyu, brown root, honeysuckle, licorice, gardenia Herbal herbal medicine composition having an effective effect on the removal of toxic components in the human body due to smoking, characterized in that it contains. To give a three hundred seconds, cranesbill, heukdu, chajeoncho, yonggyu, Puerariae Radix, honeysuckle, licorice root, gardenia, after the Schisandra respectively dry powder of purified water to the dry powder, respectively, a lower alcohol or a mixture of product and a mixed solvent of C _{1 -4} The extract obtained by filtration and concentration under reduced pressure is freeze-dried by 1 weight each for extracting herbal medicines. Preparing two dry powders of tortoise, acid powder, and Tobok-ryeong in addition to the three hundred scents, lichen herb, black bean, chajeoncho, yonggyu, brown root, honeysuckle, licorice, gardenia, and Schisandra chinensis powder, preparing each dry powder for each 10 kg; Each of the dry powders prepared in the above step was mixed with 10 times the purified water, C _1-4 lower alcohol, or a mixed solvent thereof with respect to the dry powder weight. Time, black bean is 12 hours at room temperature, the remainder is stirred for 5 hours at 95 ℃ or more to obtain a product; Centrifuging the products obtained in the above step and leaving the solid residue for 1 day, and then filtering the supernatant; Concentrating the extract obtained in the above step under reduced pressure at a temperature of 55 ℃ at 70 ~ 76 mmHg pressure to obtain a solid component; The solid components of the three hundred seconds, lichen grass, chajeoncho, brown root, tofu, pesticide, Tobokyeong, honeysuckle, licorice, gardenia, Schisandra chinensis obtained in the above step contains 60% solids, 40% solids of black bean, The solid component of is concentrated to 55% of the solid content of three hundred seconds, algae grass, honeysuckle, gardenia extract 6kg ~ 7kg extract, black bean 7kg ~ 8kg extract, chajeoncho, brown root, tofu, mountain medicine, Tobokyeong 4kg ~ Extracting 5 kg of extract, yonggyu, licorice, Schisandra chinensis extract of 5 kg to 6 kg; Chinese herbal medicine manufacturing method having an effective efficacy in removing toxic components in the human body due to smoking, characterized in that it comprises the step of fermenting for 40 days while maintaining the 30 ℃ ~ 40 ℃ by mixing and stirring each extract obtained in the step. delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete delete

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