KR100816264B1 - Cosmetic composition for alleviating skin irritation containing throat extract as an active ingredient - Google Patents

Abstract

The present invention is a neck ( Akebia It provides a cosmetic composition for alleviating skin irritation comprising a quinata extract as an active ingredient. The throat extract of the present invention significantly relieves skin irritation, has an increased anti-inflammatory activity, and when used in cosmetics, it has an excellent skin irritation relieving effect and is also useful as a cosmetic supplement.

Throat extract, skin irritation relief, cosmetic composition, supplement

Description

Cosmetic composition for relieving skin irritation containing neck extract as an active ingredient {Cosmetic Composition for Abirritating Skin Containing Extract of Akebia quinata as Active Ingredient}

The present invention relates to a cosmetic composition for skin irritation relief.

Cosmetics are used for cleansing, maintaining a healthy state and beautifying the skin, but the cosmetic composition includes ingredients necessary for the manufacture and maintenance of the product contrary to this purpose. These are ingredients that are indispensable for preserving, maintaining, and functioning cosmetics such as preservatives, surfactants, sunscreens, and whitening agents, and may cause skin irritation in some of the products actually used. Therefore, cosmetics manufacturers are making efforts to minimize the irritation by applying commonly used irritant substances to the product.

Skin irritation that may occur with the use of cosmetics includes irritation such as itching, stinging and burning, and erythema and edema. These stimuli are called anti-inflammatory drugs that act to alleviate or eliminate inflammation as a phenomenon of the inflammatory response. To date, non-steroidal substances that are used for anti-inflammatory purposes include flufenamic acid, ibuprofen, benzydamine, indomethacin, and prednisolone as a steroid system. prednisolone) and dexamethasone (Kim Chang-jong, Pathophysiology, Hallym Co., p61-69, 1988), allantoin, azulene, ε-aminokiproic acid, hydrocortisone (v), licoriceic acid and its derivatives (β) -Glycylinic acid, Glychilechin acid derivatives, etc. are known to be effective in anti-inflammatory (Ministry of Justice, Department of New Cosmetics, Namsan Sugar, p162, 1993), but indomethacin cannot be used in cosmetics and hydrocortisone This is limited, licorice acid and its derivatives are difficult to stabilize or poor solubility is difficult to achieve a practical effect due to the limitation of concentration in actual application It has a point.

The present inventors conducted a thorough study on natural products in order to compensate for the above disadvantages, and found that the neck extract is a substance having an effect of stabilizing the formulation, reducing side effects caused by cosmetics, and alleviating skin irritation. Completed.

Therefore, it is an object of the present invention to provide a cosmetic composition for skin irritation relief comprising the extract of the throat as an active ingredient.

Other objects and advantages of the present invention will become apparent from the following examples and claims.

According to another aspect of the present invention, the present invention provides a cosmetic composition for alleviating skin irritation, comprising a throat extract as an active ingredient.

The barrel used as an active ingredient of the present invention is a perennial vine belonging to the ivy ( Akebia) quinata ) is a dry stem, used as an anti-inflammatory, diuretic, analgesic, and pear pesticide in oriental medicine. It is used for nephritis, heart disease, swelling, neuralgia, arthritis, and pregnancy.

The neck extract can be a variety of extraction solvents, such as water, anhydrous or hydrous lower alcohols (methanol, ethanol, propanol and butanol) having 1 to 4 carbon atoms, acetone, ethyl acetate, chloroform, 1,3-butylene glycol and butyl It can be obtained from the barrel as an extraction solvent selected from the group consisting of acetate. More preferably, the extract of the present invention is obtained using a hydrous lower alcohol, most preferably ethanol. On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained extracts having substantially the same effect using not only the above-described extraction solvent but also other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-mentioned extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The active fraction is also included in the extract of the present invention.

According to a preferred embodiment of the present invention, the content of the extract is 0.001-5.0% by weight relative to the total weight of the cosmetic composition. If the content is less than the expected effect is difficult to appear, if the content is exceeded the effect is no longer increased.

Throat extract of the present invention to alleviate the skin irritation caused by external substances and has an anti-inflammatory activity, to mitigate the skin irritation caused by cosmetic raw materials. Therefore, the throat extract of the present invention can be used as a cosmetic supplement to alleviate skin irritation caused by cosmetic raw materials.

Raw material causing skin irritation in the cosmetic composition includes a natural extract and ingredients that can be commonly used in cosmetics. Representative raw materials that cause skin irritation include, but are not limited to, lactic acid, glycolic acid, salicylic acid and retinoic acid.

The cosmetic composition of the present invention is prepared as a composition comprising a neck extract as an active ingredient, and may be applied before or after applying the cosmetic composition having a large skin irritation to the skin. In addition, the extract may be applied to the skin by additionally added to the cosmetic composition having a high skin irritation.

The ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions in addition to the neck extract as an active ingredient, and include, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings, And carriers.

Cosmetic compositions of the present invention may be prepared in any formulation conventionally prepared in the art and include, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The present invention improves skin safety of cosmetics by relieving skin irritation that may occur when cosmetics are applied to the skin by using a throat extract.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those of ordinary skill in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident.

Preparation Example 1 Preparation of Bark Extract

The neck was washed with purified water and then dried and broken into small pieces, to which 70% lower alcohol, 1-10 times its dry weight, was added. The mixture was extracted at 15-35 ° C. for 5 days, filtered through a 300 mesh filter cloth, filtered through Whatman No. 5 filter paper, and then dried using a rotary evaporator to obtain a dry weight of 63 g / kg. The dried neck extract powder was used dissolved in 30% 1,3-butylene glycol.

Experimental Example 1 Test of the Relaxation Effect of Cellulose Extracts by Lactic Acid

Fibroblasts (Korea Cell Line Bank), which are human skin cells, were cultured in T-75 flasks (Falcon, USA) until 90% growth. This was again transferred to 96 × well plates (Falcon, USA) to 3 × 10 ⁴ cells / well and incubated for 24 hours. After culturing, the microscope confirmed whether the cells were completely attached and grew well. The experiment was performed using lactic acid as a source of skin cell stimulation. At this time, lactic acid was used as 0.2% (all skin cells were killed at this concentration). That is, 200 μl of DMEM (Sigma, US) medium containing 0.2% lactic acid was added to each well of a 96 well plate, and the neck extract was adjusted to 10, 20, 50, 100 and 200 μg / ml. In this case, no lactic acid and extract were added to the control group, and only the lactic acid was added as a comparative group. Twelve μl of MTT reagent was added to each well to compare cell viability after 12 hours of addition of test material.

In order to determine the cell viability after 4 hours, absorbance at 570 nm was measured using an ELISA reader, and cell viability was calculated according to Equation 1 below, and the results are shown in Table 1.

Cell viability (%) = [(control absorbance-test material absorbance) / control absorbance] × 100

Concentration of extract (µg / ml) Cell survival rate (%) Experimental group Comparison Control 10 27.1 0.0 100.0 20 56.2 50 83.4 100 97.6 200 103.5

In the results of Table 1, it was found that when the neck extract was treated to the lactic acid treated cells, the survival rate of the cells increased depending on the concentration of the extract, and the neck extract was relieved of cell stimulation caused by the lactic acid.

Experimental Example  2: anti-inflammatory effect test of neck extract

In order to measure the anti-inflammatory effect of the neck extract, the anti-inflammatory effect was measured in 20 people with no skin disease or skin allergy symptoms. The age distribution was 21-35 years old and the average age was 29 years old. The test method was as follows. After measuring three measurement locations on the upper arm, the erythema (a * value) of the skin was measured with a chromameter. 5% neck extract (experimental group) and licoriceic acid (comparative group, conventional anti-inflammatory agent) were applied 4 times at 1 hour intervals in two measurement locations, and the other one (control) was not applied. After 20-30 minutes of last application, the measurement site was lightly wiped with running water.

When the water was dried on the upper arm, 3 mM methyl nicotimate (0.04%) was soaked in 80 μl of paper disk, and placed for 1 minute and 30 seconds. After 1 minute and 30 seconds, the paper disk was removed and washed with running water. After 20-30 minutes of inflammation, erythema develops. The incidence of erythema is measured by chromatometer and the degree of erythema suppression is evaluated by comparing with erythema at the beginning of the experiment. Evaluation of the anti-inflammatory effect was performed as in Equation 2 below, and the results are shown in Table 2.

Anti-inflammatory effect division Δa * (after treatment-before treatment) Erythema production rate (%) Anti-inflammatory effect (%) Control 1.54 100 - 5% licorice 1.18 73.3 26.7 5% neck extract 0.57 37.0 63.0

Through the experimental results of Table 2, it can be seen that the anti-inflammatory effect of the neck extract of the present invention is far superior to the conventional anti-inflammatory licorice.

On the basis of the experimental example, it proposes a cosmetic composition of various solubilizing agent type and emulsifier type containing a throat extract excellent in skin irritation relief effect. Although this is described in detail in the following formulation example, the composition of the present invention is not limited only to the following examples.

Formulation Example 1: Softener (Skin Lotion)

As shown in Table 3 below, the softening lotion containing the barrel extract was prepared according to a conventional method.

ingredient Content (% by weight) Throat extract 2.0 glycerin 5.0 1,3-butylene glycol 3.0 PEG 1500 1.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaluronate 5.0 ethanol 10.0 Octicdodeceth-16 0.2 Polysorbate 20 0.2 incense 0.02 Uniside-U 13 0.01 Green # 13 0.001 Distilled water Remaining amount Sum 100

Formulation Example 2. Converging Cosmetic Water

As shown in Table 4 below, astringent cosmetic water containing a throat extract was prepared according to a conventional method.

ingredient Content (% by weight) Throat extract 2.0 glycerin 2.0 1,3-butylene glycol 2.0 Allantoin 0.2 DL-panthenol 0.2 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaluronate 3.0 ethanol 15.0 Polysorbate 20 0.3 Witch Hagel Extract 2.0 Citric acid a very small amount incense 0.02 Uniside-U 13 0.01 Green # 13 0.001 Distilled water Remaining amount Sum 100

Formulation Example 3. Nutrients

As shown in Table 5, nutritious longevity containing the neck extract was prepared according to a conventional method.

ingredient Content (% by weight) Throat extract 2.0 Glyceryl Stearate SE 1.5 Cetearyl Alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Cured Vegetable Oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoine 2.0 Dimethicone 0.8 Tocopherol Acetate 0.5 Carboxyvinyl Polymer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 Sodium hyaluronate 5.0 Triethanolamine 0.12 incense 0.01 Uniside-U 13 0.02 Green # 3 0.003 Distilled water Remaining amount Sum 100

Formulation Example 4. Nutrition Cream

Nutritious cream containing a throat extract as shown in Table 6 below was prepared according to a conventional method.

ingredient Content (% by weight) Throat extract 5.0 Lipophilic monostearate 2.0 Cetearyl Alcohol 2.2 Stearic acid 1.5 Beeswax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Cured Vegetable Oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoine 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaluronate 4.0 incense 0.01 Uniside-Y13 0.02 Green # 3 0.003 Distilled water Remaining amount Sum 100

Formulation Example 5. Massage Cream

A massage cream containing a throat extract as shown in Table 7 below was prepared according to a conventional method.

ingredient Content (% by weight) Throat extract 4.0 Lipophilic Monostearic Acid Glycerin 1.5 Cetearyl Alcohol 1.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Isostearyl Isosterate 5.0 Squalane 5.0 Mineral oil 35.0 Dimethicone 0.5 Hydroxyethyl cellulose 0.12 glycerin 6.0 Triethanolamine 0.7 incense 0.01 Uniside-Y13 0.02 Green # 3 0.003 Distilled water Remaining amount Sum 100

Formulation Example 6. Essence

Essence containing the throat extract as shown in Table 8 below was prepared according to a conventional method.

ingredient Content (% by weight) Throat extract 4.0 glycerin 10.0 Betaine 5.0 PEG 1500 2.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl cellulose 0.1 Sodium hyaluronate 8.0 Carboxy Vinyl Polymer 0.2 Triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeceth-16 0.4 ethanol 6.0 incense 0.02 Uniside-U 13 0.01 Green # 3 0.001 Distilled water Remaining amount Sum 100

Formulation Example 7 Pack

As shown in Table 9 below, a pack containing a barrel extract was prepared according to a conventional method.

ingredient Content (% by weight) Throat extract 2.0 Polyvinyl alcohol 15.0 Cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 Chicdestrin 0.15 DL-panthenol 0.4 Allantoin 0.1 Glycyrisin Monoammonium 0.3 Nicotinamide 0.5 ethanol 6.0 PEG 40 Cured Castor Oil 0.3 incense a very small amount Uniside-Y13 0.02 Green # 3 0.003 Distilled water Remaining amount Sum 100

Experimental Example 3: Human Skin Patch Test

Rock, which is a skin irritant in the cosmetic formulation of Formulation Example 4, containing 5.0 wt% of neck extract, targeting 20 women in their 20s and 30s who have never had hypersensitivity to skin irritation due to past history and have no skin disease or skin allergy symptoms. Tic acid 5.0% by weight was added as an irritant (Example), and the irritation-relieving effect was confirmed through a human skin patch test. At this time, the group without addition of the lactic acid and the throat extract was used as a control group, the neck extract included in the cosmetic formulation of Formulation Example 4 was replaced with purified water and only the lactic acid was added as Comparative Example 1, and included in the cosmetic formulation of Formulation Example 4 Comparative Example 2 was used to replace the neck extract extracted with 5.0% by weight licorice.

First the test site was wiped with 70% ethanol and dried. 15 μg of the prepared test substance was added dropwise into a Fin chamber (Finn chamber, 100 × 10, EPITEST, Finland), and then airtightly wrapped inside the forearm of the test subject. The patch was applied for 24 hours, the patch was removed, and the test site was marked with a marker pen. 1 hour and 24 hours after each removal (1 hour after removal, 24 hours = 24 hours, 48 hours including patch attachment) using a magnifying glass (8MC-150, DAZOR, United States) to observe the test site for erythema and edema Was observed.

The skin reaction was determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG), and the average skin reactivity was calculated by Equation 3 below and the results are shown in Table 10.

 ※ Evaluation criteria and score of skin reaction

Symbol score Evaluation standard -  0 No response, normal skin ±  0.5 Faint or light erythema +  1.0 Boundary but weak erythema, edema and papules ++  2.0 Pronounced erythema, papules and vesicles +++  3.0 Severe erythema and vesicle formation ++++  6.0 Cannon formation

Human skin patch test result Test substance 24 hours 48 hours Average skin reactivity (n = 20) ± + ++ ± + ++ Control 2 - - --- 0.41 Comparative Example 1 13 3- 3 2- 5.41 Comparative Example 2 9 2- 3 1- 3.75 Example 4 2- One - - 1.87

As can be seen from the results of Table 10, the example containing 5.0% by weight of the neck extract is significantly superior to the relaxation effect of the skin irritation induced by lactic acid, compared to Comparative Example 2 containing 5.0% by weight licorice And it was found.

Experimental Example 4: Stability test of the formulation of the cream containing the throat extract

The stability according to the temperature change of the cosmetic formulation of the present invention was tested. Nourishing cream of Formulation Example 4 containing the throat extract, cream (Comparative Example) replacing the neck extract with 5.0 wt% licorice in Formulation Example 4, and the cream (control) replaced with purified water in an opaque glass container After storage for 1 week in a constant temperature bath maintained at 45 ℃, and also kept in a opaque glass jar at a constant 4 ℃ and stored in a completely shaded refrigerator for 1 week, the degree of discoloration was measured. The results are shown in Table 11 below. At this time, the degree of product discoloration was evaluated by classifying into the following six grades.

※ criteria for evaluation of product discoloration

score Evaluation standard 0  No change One  Very little change 2  Little change 3  Slightly worse 4  Badly changed 5  Extremely severe

Safety test results of the formulation Temperature Discoloration degree of test substance Formulation Example 4 Control Comparative example 45 ℃ 0 0 4 4 ℃ 0 0 2

As can be seen from the results of Table 11, the cream of Comparative Example containing licorice acid instead of the neck extract was weak in the stability of the formulation, the cream of Formulation Example 4 containing the neck extract is hardly discolored, the conventional cosmetics Compared to the stability was found to be very excellent. Discoloration did not occur in the control case where the neck extract was replaced with purified water, indicating that discoloration did not occur by the other ingredients of the cream.

As described in detail above, the present invention provides a cosmetic composition for alleviating skin irritation, comprising a throat extract as an active ingredient. The neck extract of the present invention significantly relieves skin irritation by cosmetic raw materials, and also shows an increased anti-inflammatory activity, compared to existing anti-inflammatory agents, and when used in cosmetics, has an excellent skin irritation relief effect. Therefore, the cosmetic composition of the present invention can enhance skin safety of the cosmetic because it can relieve skin irritation that may occur when the cosmetic is applied to the skin by using a neck extract.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that the specific technology is merely a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (5)

Throat Extract ( Akebia Cosmetic composition for skin irritation relief containing quinata ) as an active ingredient. The method of claim 1, wherein the barrel extract is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol or butanol), acetone, ethyl acetate, chloroform, 1,3-butylene glycol and butyl acetate Cosmetic composition, characterized in that extracted using a solvent selected from the group consisting of. delete The cosmetic composition according to claim 1, wherein the throat extract is contained in an amount of 0.001-5.0% by weight based on the total weight of the cosmetic. The composition of claim 1 wherein the cosmetic composition consists of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Cosmetic composition, characterized in that it has a formulation selected from the group.