KR100736176B1 - Nematicidal Compositions Containing Tartaric Acid - Google Patents

Abstract

The present invention relates to a novel nematicide composition having an excellent nematicidal effect, and more particularly, provides an nematicide composition containing insecticide and tartaric acid which acts together with the insecticide to exert the nematicide effect.

Tartaric acid, nematicide

Description

Nematicidal Compositions Containing Tartaric Acid}             

1 is a graph showing the nematicidal effect of each compound to be mixed with the pesticide [A: untreated. B: 1% tartaric acid, C: 1% sodium stannate, D: 1% sodium citrate]

2 is a graph showing the insecticidal effect according to the nematicide composition [A: untreated, B: 1,000 times imidacloprid liquid, C: 0.1% TA + imidacloprid liquid, D: 1,000 times of abamectin emulsion, E: 0.1% TA + ava Mectin emulsion 1000 times, F: phospham solution 1,000 times, G: 0.1% TA + phospham solution 1,000 times, H: phosphazate solution 1,000 times, I: 0.1% TA + phosphate solution 1,000 times]

Figure 3 is a graph showing the nematicidal effect according to the mixed concentration of tartaric acid [A: no treatment, B: 1,000 times phosphiteate solution, C: 1,000 times 0.1% TA + phosphate solution, D: 0.01% TA + phosphase Thousand liquid 1,000 times]

4 is a graph showing the nematicidal effect according to the dilution factor of the phenytrothion emulsion

Figure 5 is a comparison photo of the lethal nematode (a) and untreated nematode (b) after treatment of the composition of the present invention

Figure 6 is a comparative photograph of the photograph (a) and the untreated group (b) in which the occurrence of root nodules by the phenytrothion emulsion treatment is suppressed

The present invention relates to a nematicide composition, and more particularly to a novel nematicide composition having a very good nematicide effect by mixing tartaric acid with a conventionally disclosed pesticide.

Nematodes derive their origins from Greek yarns ((nema (= sil) + oid (= like)). The composition ratio is 50% sea nematodes, 15% animal parasites, 25% free live nematodes, Phytoparasites are known to be 10%.

Pine reptiles ( Bursaphelenchus xylophilus Steiner & Buhrer (1934), Nickle, 1970) first appeared in Japan in 1905, killing most of Japan's pine forests, and China was confirmed in 1982 and Taiwan in 1985. In Korea, as the internationalization and liberalization era increased, international trade volume and diversified trade products prevented the inflow, and the first damage occurred in Busan in 1988. As of the end of 2003, 160,999 pine trees were felled in 28 municipalities. Therefore, the development of preventive drugs is urgently needed to preserve pine forests by preventing damage caused by pine re-nematodes.

Pine bar nematode preventive drugs are used as tree injection drugs in Japan. Levamis, Mesulfenfos and Morantel tartrate have been developed and used in Japan. It is impossible to introduce and use.

Root-knot nematodes are plant parasitic nematodes, which invade the roots of plants to absorb nutrients and promote infection of pathogenic microorganisms. Due to recent plant cultivation, the damage of nematodes is increasing due to year-round cultivation of the same crops. Root-knot nematodes have more than 700 species of host plants, which are rare in resistant varieties, and it is urgent to develop new control methods because they are difficult to control due to resistance of chemical insecticides.

Biological control methods of plant parasitic nematodes include methods using predatory nematodes, predatory insects, mites, bacteria, filamentous fungi, etc. Among them, the most effective method is by predatory or parasitic fungi. This control method has the advantage that there is no problem of pesticides, soil pollution and pesticide residues caused by spraying pesticides. However, these biocontrol agents have a very low control effect compared to chemical pesticides, so crop cultivators tend to prefer chemical pesticides.

The present invention has been proposed to solve the problems of the prior art, the purpose of which is very excellent in the lethal effect on nematodes, and also can prevent the damage of pine ash nematode disease when injecting trees on pine, etc. A useful nematicide composition is provided.

In order to achieve the above object, the present invention provides an insecticide composition comprising a pesticide and tartaric acid that acts together with the insecticide to exert an insecticidal effect.

Insecticides may include any pesticide that exerts an insecticidal effect when applied together with tartaric acid. It may include any insecticide that can exert a sufficient insecticidal effect on its own, and the insecticidal effect on nematodes is very weak. Examples of the former include abamectin emulsion, emamectin benzoate emulsion, phosphazate solution, imidacloprid emulsion, and the like. Examples of the latter include phenytrothion emulsion and the like. These insecticides may be mixed with tartaric acid alone, or two or more components may be mixed and added together with tartaric acid. Abamectin is a derivative of natural ingredients extracted from microorganisms and is safe for the environment, and exhibits insecticidal effects by contact poisoning and digestive poisoning. This medicine is known to be effective in controlling mites, such as roses and watermelons, due to its rapid effect and accurate effect. Emamectin benzoate is a natural derivative derived from soil bacteria and shows strong permeability and rapid insecticidal effect. It is known to be widely used for controlling insect pests and target crops, such as cucumber locusts, beetroot moths and Chinese cabbage moths. Imidacloprid solution is a chloronicotinyl family of penetrating and long lasting drugs. This drug has a wide range of pests and crops, and is a pine needle injection agent specifically for the control of pine needles and bark beetles.

In addition, phoschiate is a nematode medicinal agent that can effectively control root-knot nematodes such as watermelon and melons, strawberry leaf nematodes of daughters, rhizome nematodes of chrysanthemums and garlic bulb nematodes as penetrating agents. According to the experimental results of the present invention, the drugs can be used in dilution, and when applied to nematodes alone, the insecticidal activity is not small or very large, whereas when mixed with tartaric acid, the insecticidal effect is remarkably improved. Confirmed.

Phenithrothion is well known as an organophosphorus insecticide that is well absorbed by plants and is effective against larvae that feed on plants. According to the experimental results of the present invention, it was confirmed that the insecticidal effect was excellent even when treated with nematodes alone. . When using phenytrothion emulsions, the dilution factor is about 500-5,000 times, preferably about 2,000-3,000 times.

Tartaric acid was found to exert an excellent nematicide effect when mixed with the pesticides exemplified above. Tartaric acid is classified into four types of isomers such as D-type, L-type, DL-type, and inactive-type. D-type is present in nature and DL-type is synthesized. D-type is used as an acidulant in grapes, lime flavored beverages, fruit wine, candy, jelly, ice cream, etc., and is used as a powder of fast-acting synthetic expanders in the form of monopotassium salt of tartaric acid (tar tar cream). Tartaric acid is used to impart aroma to fruit or fruit flavored foods, as a powder in baking powders, as a discoloration inhibitor in cheeses, and as a metal remover in vegetable and vegetable oils, but it has not been reported as a pesticide. In the present invention, any form of tartaric acid may be used as an nematicide irrespective of the specific form of tartaric acid.

The concentration in the composition of tartaric acid mixed with the pesticide is not required to be particularly limited, for example, in the range of 0.1-20% (w / v), preferably 0.1-10% (w / v), more Preferably, the activity was found to be better at 0.1% (w / v).

Hereinafter, the content of the present invention will be described in more detail with reference to Examples. However, these examples are only presented to understand the content of the present invention, and the scope of the present invention should not be construed as being limited to these embodiments.

<Example 1> Selection of nematicide mixed compound

In order to select nematicide compounds, nematicidal activity was examined using tartaric acid, sodium tartarate and sodium zincate. The nematodes used in the experiment were collected from the pine reef infected with pine ash nematode in Jinju area and brought to the laboratory and isolated by funnel method, and then cultured using gray fungus.

The test compound was placed in a disposable petri dish 5 cm in diameter and inoculated with 0.2 ml of nematode suspension (200 nematodes) and treated at 25 ° C. After 24 hours of treatment, the nematode numbers were examined and repeated three times for each treatment.

The results of FIG. 1 show that the insecticidal activity of the compound was treated, and the insecticidal effect of the tartaric acid treatment group was 70% and the sodium tartarate treatment group was 32%, but the sodium citrate treatment group showed no insecticidal effect.

Example 2 Insecticidal Activity of Insecticide Mixed with Tartaric Acid

Mixed Effect of Tartaric Acid (TA) by Insecticide

In order to examine the nematicidal activity by the mixed treatment of tartaric acid by insecticide, phosphazate 30% (w / v) solution, phosphamidone 50% (w / v) solution, imidacloprid 4% (w / v) solution, The nematicidal effect was investigated by mixing tartamic acid with 0.1% (w / v) concentration of abamectin 1.8% (w / v) emulsion.

2 shows that the nematicidal activity was very low when the co-agent was treated alone as a result of examining the nematicidal effect of the drug mixed with tartaric acid, but the insecticidal activity was very high when the tartaric acid was mixed. In particular, nematicidal activity was very high when tartaric acid was mixed with a 1,000-fold dilution of 30% (w / v) phosphazate. The insecticidal rate was 10% or less when the 1,000-fold dilution solution of phosphazate solution was treated alone, but when the tartaric acid was mixed with 0.1% (w / v) concentration, the insecticidal rate was 90% or more.

Nematode Effect by Tartrate Mixture Concentration

From the above results, in order to examine the nematicidal activating effect in the case of using a mixture of high nematicidal activity, phosphitate solution was mixed and investigated with tartaric acid at 0.1% (w / v) and 0.01% (w / v) concentrations. As a result, as shown in FIG. 3, the insecticidal rate was 10% or less in the phosphazate solution alone treatment group, but the insecticidal effect was high when the mixture was treated with tartaric acid. The insecticidal effect was very high, with the pesticidal rate of 90% or more in the mixture treated with tartaric acid at a concentration of 0.1% (w / v), and the insecticide rate was 10% in the 0.01 (w / v)% treatment. The nematicidal effect of the mixed treatment and the non-tartrate treatment at the concentration of 0.1% (w / v) of tartaric acid is shown in FIG. 5.

<Example 3> Nematode control effect of phenytrothion 50% (w / v) emulsion

Nematode Effect of Phenytrothion 50% (w / v) Emulsion

To examine the nematicidal effect of phenytrothion 50% (w / v) emulsion, the pine ash nematode was diluted by 1,000, 2,000, and 5,000 folds. The pear dilution showed 100% mortality after 1 day of treatment, and even the 5,000-fold dilution showed 85% of nematode lethal effect.

Nematode Control Pot Test of Phenytrothion 50% (w / v) Emulsion

Inhibition of nematode density was investigated by irrigating phenytrothion emulsions to root-knot nematode soils. As a result, the density of root-knot nematodes was significantly reduced. In the phenyl trothion 2,000-fold dilution, the treatment showed a 95.1% density reduction, and in the 4,000-fold dilution, the treatment showed an 89.6% density reduction. In addition, in the root-knot-forming water, there was 13 in the untreated group, whereas one was formed in the 2,000-fold dilute solution of phenytrothion, and the damage suppression effect of the root-knot nematode was very high (see FIG. 6).

TABLE 1

process Nematode density before treatment (mari / 100 g soil) Nematode density after treatment (/ 100 g soil) Reduction rate (%) Root Bone / Object  Phenytrothion emulsion 2,000 times 800  39 95.1 0.9  Phentrothion emulsion 4,000 times 83 89.6 9.3  4,000 phosphate solution (control)  21 97.4 0.1  Nematode 1,020 0 12.9

Soil Irrigation Treatment of Phenytrothion 50% (w / v) Emulsion

Phenytrothion emulsions were irrigated with soil to investigate the damage of cucumbers by treatment amount. As a result, the drug treatment did not affect the germination of cucumber seeds, but after germination, weakening occurred according to the dosage. That is, in the 1,000-fold dilution treatment treatment, there was no damage in the first irrigation treatment, but there was a weakening of the mozalloc symptoms in the two irrigation treatment treatments. However, in the 2,000 and 4,000-fold dilution treatments, no damage occurred even after two irrigation treatments.

TABLE 2

process Sowing Germination rate (%) Damage rate by treatment count (%) 1 time treatment (just after sowing) 2 times treatment (1 week after sowing)  Phenytrothion emulsion 1,000 times 30 tablets 93.3 0 75.6  Phenytrothion emulsion 2,000 times 30 tablets 90.0 0 0  Phentrothion emulsion 4,000 times 30 tablets 96.7 0 0  No treatment 30 tablets 86.7 0 0

The effects of soil irrigation of phenytrothion emulsion on the growth of crops were investigated. As a result, the treatment of 2,000 and 4,000-fold diluents with phenytrothion did not significantly affect the growth of cucumbers. .

TABLE 3

process Growth situation Ground length (cm) Ground biometric weight (g)  2,000-fold dilution of phenytrothion emulsion 11.5 1.9  4,000-fold dilution of phenytrothion emulsion 11.2 1.4  4,000-fold dilution of phosphazate solution (control) 9.2 0.9  No treatment 11.0 1.3

Example 4 Effect of Tree Injection of Abamectin and Emamectin Benzoate Containing Tartaric Acid

A stock solution of abamectin 1.8% (w / v) emulsion and emamectin benzoate 2.15% (w / v) emulsion was injected with 10 ml and 20 ml per 10 cm of the height of chestnut, and 0.1% (w / v) on the other hand. v) The drug was diluted 10-fold in tartaric acid, and then 100ml and 200ml of wood was injected per 10cm of the height of the pine tree. As a result, the nematode infection rate was 40% in the pine ash nematode alone treatment group, but the nematode damage did not occur in the tree inoculated with 100ml and 200ml of 10-fold dilutions of abamectin emulsion and 10-fold dilutions of emamectin benzoate. In addition, 20 ml of abamectin emulsion solution and 10 ml of emamectin benzoate emulsion solution showed 3.3% incidence of nematode damage and 91.8.

TABLE 4

Treating medicine Dilution drainage Treatment amount / Chest height 10㎝ Treatment head Nematode Infection Rate (%) Control Abamectin Emulsion -     10   30 0 100 -     20   30 3.3 91.8 10    100   30 0 100 10    200   30 0 100 Emamectin Benzoate Emulsion -     10   30 3.3 91.8     20   30 0 100 10    100   30 0 100 10    200   30 0 100 Nematode - - 30 40 -

The nematicidal composition according to the present invention has a very excellent lethal effect on nematodes, and is also very useful in the forestry industry because it can prevent the damage of pine ash nematode disease when injecting trees on pines and the like.

Claims (3)

Contains at least one pesticide selected from abamectin emulsion, emamectin benzoate emulsion, phosphazate emulsion, imidacloprid emulsion, and phenytrothion emulsion, and tartaric acid which acts together with the insecticide to exert nematicidal effect. Nematicide composition delete The nematicide composition according to claim 1, wherein the concentration of tartaric acid is 0.1-20% (w / v).

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