KR100639833B1 - A edible herbs using younger barley leaf whcih have an antioxidant activity and a method for preparation thereof - Google Patents

Abstract

The present invention relates to an antioxidant active edible sprout using young barley leaves and a method of producing the same. The content of saponarin and isobitexin by sowing young barley leaves in autumn and harvesting them during wintering, that is, when the main leaves are five leaves. This is very high and therefore has an excellent effect of providing edible herbs which also exhibit high antioxidant activity. In addition, the edible herb of the present invention is a very useful invention in the food industry, cosmetic industry and pharmaceutical industry because it can provide functional cosmetics and antioxidants containing the extract as an active ingredient using the extract.

Wheat, barley, saponarin, isobitexin, electron donating ability, antioxidant activity

Description

A edible herbs using younger barley leaf whcih have an antioxidant activity and a method for preparation

Figure 1 shows the young barley leaves extracted with MeOH and injected into HPLC.

Figure 2 shows the standard calibration curve of saponarin components by HPLC from young barley leaves.

Figure 3 shows the standard calibration curve of the isobitexin component by HPLC from young barley leaves.

Fig. 4 shows the correlation between the saponarin component and the isobitexin component when five main leaves before wintering.

FIG. 5 shows the correlation between saponarin and isobitexin components and EDA activity when five pre-wintering leaves were from young barley leaves.

The present invention relates to antioxidant active edible herbs using young barley leaves and a method for producing the same. More specifically, young barley leaves are sown in autumn and harvested when five main leaves are harvested. The present invention relates to edible herbs and a method of producing the same, which are very high in content and thus also have high antioxidant activity.

In recent years, natural plants have received a lot of attention as a source of bioactive substances including antioxidants, anti-inflammatory, anti-cancer function. Antioxidants are known to physically stabilize the membrane itself or act as a chain stopper in the peroxidation of lipids (Yagi, 1987; Harman, 1982; Culter, 1984). To detox, it is very important to take antioxidants such as β-carotene, vitamin E, vitamin C, uric acid, glutathionine and riboflavin. In addition, antioxidants have been identified as a system that prevents the generation of free radicals against damage caused by free radicals, a system that quickly stabilizes and stabilizes free radicals, and repairs and regenerates free radical damage. It is known that the higher the E, SOD activity, the longer the life.

Conventionally, synthetic antioxidants such as BHA and BHT have been used as foods, but soon their use began to be restricted due to their toxicity, thus increasing the importance of natural antioxidants.

On the other hand, sapononarin and isobitexin components found in barley leaves have been identified as flavone-based antioxidants (Ohkawa, 1998; Osawa, 1992), flavones contained in cereals, vegetables and fruits. Antioxidants of the system is a component of much interest as a naturally occurring substance to protect the living body from the risk factors caused by free radicals. In particular, young barley leaf extract is known to have antioxidant activity such as α-tocopherol as well as anti-inflammatory activity (Osawa, 1992), and its industrial applicability is increasing. However, studies related to saponarin and isobitexin of young barley and wheat leaves are still insignificant throughout Korea and abroad.

In view of the above, the present inventors quantify the saponarin and isobitexin by dividing young barley leaves and wheat leaves before and after wintering, and analyzing the antioxidant activity of young barley leaves by saponarin and iso After investigating the correlation between the bitexin content and the antioxidant activity, the present invention was completed by confirming that young barley leaves before wintering had a high content of these substances and thus exhibited high antioxidant activity.

Accordingly, it is an object of the present invention to provide an edible herb having antioxidant activity and a method of producing the same using young barley leaves.

In order to achieve the above object, the present inventors sow the leaves of 23 varieties of barley and 7 varieties of wheat in autumn and harvest them separately after wintering and after wintering, and then dry and pulverize them to make a sample for extraction, and the sample and MeOH. The mixed solvent was filtered and concentrated under reduced pressure, and then saponarin and isothexin were simultaneously quantified by HPLC, and the content difference was compared between varieties according to plant species and harvest time, and then sampled from young barley leaves. Antioxidant activity by the electron donating ability according to different concentrations was measured and compared, and the correlation between saponarin content and isobitexin content and antioxidative activity when 5 main leaves were wintered before harvesting Completion of the present invention by confirming that the young barley leaves contain much more saponarin and isobitexin components and also have higher antioxidant activity. It was.

Hereinafter, the configuration of the present invention will be described in detail.

The present invention is sowing the leaves of 23 varieties of barley and seven varieties of wheat in the fall season and harvested separately after wintering, after wintering, and then drying and grinding to prepare a sample; The sample and MeOH were mixed well and extracted in a shaker for 9 hours, the extracted solvent was filtered and concentrated under reduced pressure, and then saponarin and isothexin were simultaneously quantified using HPLC, and the cultivars were mixed according to plant species and harvest time. Comparing; Comparing and measuring the antioxidant activity due to the electron donating ability according to the varieties between the sample concentrations from young barley leaves; And investigating the correlation between saponarin content and isobitexin content when the main leaves were five sheets before wintering, and the correlation between the components and antioxidant activity.

Hereinafter, the configuration of the present invention will be described in detail by way of examples, but the scope of the present invention is not limited thereto.

Example 1 Experimental Materials and Sample Preparation

The materials disclosed in the present invention are vulture barley, baekbark barley, saenggang barley, miracle barley, barley barley, ol barley, Taepyeong barley, health barley, paldori barley, wheat sheep barley, tapgol barley, almond barley, Daejin barley, May barley, sae barley , Barley, large barley No. 1, large barley, evergreen barley, new barley, waxy barley, large barley and barley of barley, as well as coarse wheat, fine wheat, white wheat, green wheat, all wheat wheat, Korean wheat and gold wheat Seven varieties of wheat were grown and used in greenhouses at Korea National Open University.

These varieties were sown on October 1, 2002, and the leaves were harvested when five main leaves emerged (before wintering) and on March 10 after wintering, respectively, and were subjected to experiments. At this time, the plants can be grown in both greenhouse or roadbed cultivation, it is good to maintain the temperature about 11 ~ 15 ℃ when greenhouse cultivation. Also, sowing can be done at any time during autumn, but it is most preferable to do so on October 1 because it takes a lot of days before leaves appear on the ground. The harvested leaves were dried in a 60 ° C hot air dryer (JISICO, Cheil Science Industry) until the moisture content was 3% or less, and then ground and used as samples for extraction.

Example 2: Simultaneous Quantitative Analysis and Comparative Study of Saponarin and Isovitex

The dried sample was ground finely, taken 10g each, and mixed into 300ml of MeOH 300ml as an extraction solvent, and the mixed solution of MeOH and MeOH was extracted in a shaker for 9 hours, and the extracted solvent was filtered. After concentration under reduced pressure, 100 ml was analyzed. Determination of saponarin and isobitexin components was carried out using HPLC (waters 501 system), UV-Vis (waters 480 detector) at 254nm, the flow rate of the solvent was 1.0mL / min Develosil ODS-5 column (4.6 × 250nm , Nomura Chem. Co., Ltd.). At this time, the standard calibration curve was prepared at the concentration of 50, 100, 200, 300, 400 ppm by purchasing the standard material of saponarin and isobitexin from Extrasynthese Co., France.

1. Simultaneous Determination of Saponarin and Isovitex in Barley Leaves

10 g of barley leaf samples were extracted with MeOH and injected into HPLC. In FIG. 1, the peak of saponarin was in the 19-minute squad and the peak of the isobitaxin was in the 23-minute squad.

In addition, the standard calibration curves of the identified saponarin and isobitexin components are shown in FIGS. 2 and 3, respectively. The calibration curve for saponarin was estimated to be Y = 259062 + 25457.27X (r ² = 0.9993), and a linear correlation was observed between concentration (X) and peak area (Y). The calibration curve for isobitaxin was estimated to be Y = -191728 + 34146.712X (r ² = 0.9993), and a linear correlation was observed between the concentration (X) and the peak area (Y).

2. Varietal Varieties of Saponarin and Isobitexin Contents by Harvest Date and Plant Species

(1) First, the differences between the varieties of saponarin content according to the harvest time and plant species are shown in Table 1 below.

Varietal Differences in Saponarin Content According to Harvest Date and Plant Species (Wheat, Barley) (Unit: mg / 100g Drying Standard) Plant species kind Harvest time Winter season After wintering wheat Eunmil Cho 6.20 - Wheat 1.17 - Anbaek Mill 1.16 - Grumille 1.17 - All-Grumille 6.99 - Our wheat 3.05 - Geumgang Mill 1.19 - barley Rushing barley 853.35 458.27 Great Barley 718.61 499.04 New River Barley 762.67 374.49 Miracle barley 518.80 467.00 Barley Barley 897.65 390.66 Olborg 906.05 457.41 Taiping Barley 693.59 395.00 Healthy barley 879.36 119.94 Paldoribo 730.33 443.33 Wheat barley 867.02 406.24 Top 747.98 417.33 Rich barley 673.82 390.62 Daejin Barley 865.96 470.02 Maypole 753.92 372.05 New barley 739.63 452.92 River barley 1177.07 355.12 Large Alvor No. 1 682.08 320.06 Large barley 638.03 338.70 Evergreen barley 680.87 371.02 Saeoli 973.27 448.90 Waxy barley 812.54 416.65 Daeyeonbori 808.59 427.08 Alvory 787.62 447.30 Average barley 789.95 (A) 401.70 (B) Difference (A-B) 388.25 ^**

**: Significant difference between pre-wintering and post-wintering at 1% level.

Comparing the saponarin content of barley leaves and wheat leaves in Table 1, it can be seen that the average saponarin content of wheat leaves before wintering is 7mg 3mg, which is much less than the average content of barley 23 varieties of 789.9mg. In the case of barley leaves before wintering, saponarin content was 518 ~ 1177mg (100g dry leaves) according to varieties. Saponarin content was not detected in wheat leaves after wintering, and saponarin content of barley leaves was reduced by about 50% in all varieties. Therefore, young barley leaves, rather than young wheat leaves, and harvesting season, it was judged that it would be advantageous to harvest and use the leaves before wintering.

On the other hand, Rapacz et al reported that the osmotic pressure is increased by increasing the water-soluble sugar content due to the cold, and injury resistance is increased. Denises et al. Start synthesizing lutonarin when saponarin increases. It has a glycoside. In particular, Jerry et al. Explained that when young barley leaves receive low-energy light such as white light, the content of saponarin increases and starts the synthesis of rutonarin.

(2) The differences between the varieties of isobitexin content according to harvest time and plant species are shown in Table 2 below.

Variation in Varieties of Isobitexin Content According to Harvest Date and Plant Species (Wheat, Barley) (Unit: mg / 100g Drying Standard) Plant species kind Harvest time Winter season After wintering wheat Eunmil Cho 31.47 25.02 Wheat 29.93 22.20 Anbaek Mill 25.05 16.36 Grumille 18.89 27.70 All-Grumille 36.53 24.06 Our wheat 26.28 20.27 Geumgang Mill 24.15 19.59 barley Rushing barley 114.16 39.15 Great Barley 69.15 44.48 New River Barley 79.21 41.99 Miracle barley 54.89 30.98 Barley Barley 115.24 26.09 Olborg 112.73 43.27 Taiping Barley 85.85 30.07 Healthy barley 107.73 63.76 Paldoribo 114.97 38.26 Wheat barley 71.72 30.01 Top 119.91 41.24 Rich barley 54.14 46.53 Daejin Barley 71.98 32.31 Maypole 95.35 34.53 New barley 71.81 34.76 River barley 119.67 30.30 Large Alvor No. 1 85.94 14.76 Large barley 110.17 50.95 Evergreen barley 66.55 32.02 Saeoli 137.22 46.66 Waxy barley 86.10 33.82 Daeyeonbori 105.71 39.26 Alvory 65.69 25.30 Average Wheat (B) 27.47 22.17 Barley (A) 92.00 36.98 Difference A-B 64.53 ^** 14.81 ^**

**: Significant difference between wheat and barley at 1% level.

Comparing the isobitexin content of young barley leaves and wheat leaves in Table 2, the average isobitexin content of young wheat leaves before wintering is 27.47mg, which is about 64.53mg of the difference in the content of 92. You can see it. After wintering, the average isobitexin content was 22.17mg, which was not significantly reduced compared to saponarin. However, barley leaf was significantly reduced after wintering to 36.98mg.

Rice seeds, on the other hand, contain phenolic compounds such as isobitexin, which have been found to be very strong antioxidants that inhibit lipid peroxidation (Ramrathnam, 1998, 1999).

Example 3 Investigation of Antioxidant Activity by Electron Donating Ability

In order to measure the antioxidant activity of young barley leaves, Blois's method was modified. That is, the dry grinding sample content for MeOH was 0.1, 0.5, 1.0% and then extracted for 24 hours. 1 ml of 0.2 M DPPH solution was added to 2 ml of the supernatant of the extract, shaken for 10 seconds with a vortexing mixer, and 30 minutes later, the absorbance was measured at 517 nm using a spectrophotometer. Here, the electron donating ability (EDA,%) to measure the degree of inhibition of oxidation by donating electrons to oxidative active oxygen causing chain peroxidation of lipid peroxidation and causing various diseases and aging is the reducing power of the sample. In the present invention, the electron donating ability in the present invention is expressed as a percentage by using the sample added absorbance in contrast to the sample addition.

The results of analyzing the antioxidant activity of the young barley leaves by EDA are shown in Table 3 below.

Comparison of EDA of MeOH Extract in Young Barley Leaves (Unit:%) kind 0.1% 0.5% 1.0% Alvory 3.42 ± 0.41 8.8 ± 1.88 76.3 ± 1.55 Top 2.56 ± 0.36 7.9 ± 1.46 77.2 ± 1.33 Olborg 0.81 ± 0.13 9.1 ± 1.32 80.6 ± 1.26 Rich barley 2.79 ± 1.10 8.5 ± 1.56 76.3 ± 1.44 Saeoli 3.11 ± 0.68 13.6 ± 1.68 78.6 ± 1.87 Daeyeonbori 4.13 ± 0.97 14.2 ± 1.83 79.1 ± 1.53 Evergreen barley 1.08 ± 0.88 14.2 ± 1.22 75.3 ± 1.83 River barley 1.87 ± 0.55 13.5 ± 1.76 80.3 ± 1.79

In Table 3, the EDA of 0.1%, 0.5% and 1.0% of the MeOH solution showed 4% or less electron donating ability at 0.1% of sample concentration, and 7 ~ 14% electron donating ability at 0.5% of sample concentration. It can be seen that the difference between the varieties was large. In addition, the EDA of the sample content of 1% showed a very high antioxidant activity of more than 75%, from the results it can be concluded that there is a large EDA difference between the varieties between sample concentrations. On the other hand, in the case of barley, not only the saponarin content and the isobitexin content are very high, but also the antioxidant activity is also very high.

Example 4 Correlation between Saponarin and Isobitexin Content and Antioxidant Activity

The correlation between saponarin content and isobitexin content when young barley leaves were 5 sheets before wintering was examined, and the results are shown in FIG. 4. In FIG. 4, the crystal coefficient of saponarin content and the total content of isobitexin showed a linear correlation with r ² = 0.7496. In general, the saponarin content was also high in the variety of saponarin content.

In addition, the correlation between saponarin and isobitexin content and antioxidant activity from young barley leaves was examined, and the results are shown in FIG. 5. In Figure 5, both saponarin and isobitexin components are shown to have high antioxidant activity in the high content varieties.

As is apparent from the above examples, the present invention is sowing the young barley leaves in autumn and harvested when five main leaves of winter solstice leaves so that the content of saponarin and isobitexin component is very high and accordingly the antioxidant activity is also high It has an excellent effect on providing edible herbs. In addition, the edible herb of the present invention is a very useful invention in the food industry, cosmetic industry and pharmaceutical industry because it can provide functional cosmetics and antioxidants containing the extract as an active ingredient using the extract.

Claims (3)

Soybean barley is sown in autumn and harvested when 5 main leaves are harvested. Method for producing antioxidant active edible herbs, characterized in that. delete Edible herbs produced by the method of claim 1.

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