KR100601191B1 - Hypoglycemic agents - Google Patents

Abstract

The present invention relates to a blood sugar lowering agent having an extract of corn whiskers as an active ingredient and a food and drink containing the same.

Description

Hypoglycemic agents {HYPOGLYCEMIC AGENTS}

The present invention relates to a blood sugar lowering agent having an extract of corn whiskers as an active ingredient and a food and drink containing the extract.

In recent years, the incidence of diabetes tends to increase with hypertension and cancer. Background of the Invention Diabetic patients are rapidly increasing due to declining glucose tolerance due to age, in addition to excessive calorie intake, lack of labor and exercise, or mental stress due to abundant diet.

Diabetes is a disease that causes chronic systemic metabolic disorders and indicates chronic hyperglycemia. It is considered that many factors, such as many environmental factors and abnormality of an insulin receptor gene or a sugar transport carrier gene, are involved. The major regulator of blood glucose is insulin synthesized and secreted from pancreatic β cells. Hyperglycemia occurs due to deficiency of insulin or excess of factors antagonizing its action. A deficiency of insulin leads to abnormalities in sugar, protein, and fat metabolism, resulting in various complications such as axosis, capillary abnormalities in the stomach and retina, peripheral neuropathy, and promoting atherosclerosis.

The goal of diabetes treatment is to alleviate the deficiency of insulin in all tissues of the system and to maintain and normalize metabolism, and to prevent and suppress these acute and chronic complications.

In the treatment of diabetes mellitus or glucose tolerance disorder, dietary therapy (calorie restriction) or exercise therapy are generally performed, and drug therapy is performed when blood glucose cannot be controlled well by these treatments. Diet and exercise therapy are becoming a great mental and physical stress for the patient. In addition, drugs such as oral hypoglycemic agents used in pharmacotherapy are required to have no side effects and to be continuously and safely ingested for a long time.

In such a situation, as a result of searching for a component having a blood sugar lowering action among foods that a person normally eats, various food components that are considered to have a blood sugar lowering action have been proposed. For example, an impaired glucose tolerance improving agent using an hemicellulose partial decomposition product extracted from corn bran has been proposed (Japanese Patent Laid-Open No. 7-324036). However, it is a reality which has not yet been satisfied with the validity.

Accordingly, an object of the present invention is to provide a novel herbal extract having a conventional hypoglycemic action.

MEANS TO SOLVE THE PROBLEM As a result of earnestly examining in order to solve the said subject, the present inventors discovered that the extract of corn beard has the outstanding hypoglycemic effect, and completed this invention.

That is, the present invention is to provide a blood glucose lowering agent using the extract of corn whiskers as an active ingredient.

In addition, the present invention is to provide a food or drink having a hypoglycemic action or a hypoglycemic action containing the extract of corn whiskers.

The present invention also provides the use of the extract of corn whiskers for the production of food and drink having a hypoglycemic or hypoglycemic action.

Furthermore, the present invention provides a method for treating diabetes, characterized by administering the extract of corn swim.

1 is a diagram showing a gel filtration chromatogram of the extract of corn whiskers.

Column: Superdex 30 prep grade

Column Size: 16 mm ID * 60cm L

Flow rate: 1.0 ml / min

Injection volume: 50 μl

Eluent: 10 mM HEPES-NaOH (pH 7.4) / 1 M NaCl

Figure 2 is a diagram showing the hypoglycemic effect of the extract of corn whiskers in STZ-induced diabetic model rats.

Best Mode for Carrying Out the Invention

Corn whiskers are the corn silk (also called "corn whiskers") of the freshening of corn Zea mays L. of Gramineae. The mountainous regions are China, Japan, and Korea. Pharmacological actions have been reported with diuresis, lowering blood pressure, and promoting bile secretion [難 破 恒 雄: 和 漢 藥 百科 圖鑑 [II]: 131, 1994, (株) 保育 社].

Corn whiskers used in the present invention may be corn extracts of all varieties cultivated all over the world, but preferred is dried products of corn whiskers derived from Adan No. 12 or Adan No. 14 corn in Shandong Province, China.

Corn beard extract can be obtained using the general method used for the extraction of active ingredients from plant herbs.

For example, corn whiskers are harvested and dried. Then extracted with solvent. For extraction, it is preferable to use about 10-30 times of water, aqueous ethanol, weakly acidic water, etc. with respect to the chopped or crushed sample. The export temperature is from room temperature to the boiling point temperature of the extraction solvent, preferably extraction by hot water. Extraction time is performed under stirring for 30 to several hours in the case of hot water. The obtained extract can be used as an extract of the corn whiskers of the present invention as it is, but in order to concentrate the active ingredient of the extract again, the extract is separated into a solid and a liquid by filtration and centrifugation, and the liquid portion under reduced pressure (below 95 ° C). You may concentrate. The concentrated liquid may be dried again by spray drying, reduced pressure drying or lyophilization to obtain a powder of whiskers. Moreover, you may refine | purify this extract again by chromatography etc. as needed.

The extract of corn whiskers thus obtained has a hypoglycemic effect alone, but a synergistic or additive effect can be expected by using it with a food extract component having another hypoglycemic effect. For example, hydrothermal extracts of cultured mycelium of Cordyceps sinensis (Berkeley) Saccard have several pharmacological effects, including cardiac, hypotensive, antitussive, anti-fatigue and hypoglycemic (WO96 / 00580). By using the extract of corn whiskers with this extract as a medicine, it is considered that various medicinal effects can be expected including synergistic or additive hypoglycemic action.

When Namba corn mustard extract is used as an antihyperglycemic agent or a drug for promoting hypoglycemic activity, appropriate excipients (lactose, starch, crystalline cellulose, etc.) may be added and mixed to prepare various formulations (tablets, capsules, powders, granules). Or granules). In addition, by adding a small amount of the extract of corn whiskers in the food and drink, without improving the flavor of the food and drink can be expected to improve or prevent diabetes in the daily diet.

When the extract of corn whiskers of the present invention is used as a blood glucose raising inhibitor or a blood glucose lowering agent as a medicine, the extract can be orally administered in the range of 0.1 to 20 g / kg of dry solids per day for adults.

Although an Example is given to this invention concretely below, this invention is not limited to these Examples.

Example 1 Preparation of Extract

After drying the corn beard (Liquid No. 12, Shandong Province, China) for 30 minutes in about 15 times the amount of water, and boiled for 1 hour to extract the active ingredient. The extraction residue was added about 8 times of water and boiled for 1 hour. The extracts were combined and filtered through 100 mesh sieve. The filtrate was concentrated under reduced pressure until the specific gravity was 1.08. The concentrate was centrifuged (20 min at 2000 r.p.m), and the supernatant thereof was heat sterilized to obtain a hydrothermal extract of corn beard. Fig. 1 shows the gel filtration chromatogram of this extract (dry solid content 3.33 g / 100 ml).

Example 2 Preparation Example of Food and Drink Containing Extract

65.6 kg of the extract of corn stubble obtained in Example 1, Cordyceps sinensis hot water extract (see WO96 / 00580), 3.33 kg (50 mg / mL of solid content), 5.0 kg of sweetener, 0.5 kg of citric acid, 0.5 kg of malic acid, and 0.1 kg of perfume The total amount was added to 100 kg and stirred and dissolved. 0.07 kg of preservative was dissolved in 0.5 L of alcohol and added. After pre-sterilization, the bottles were filled and immersed in hot water at 90 ° C. for 2 hours. After that, the product was cooled by natural cooling overnight.

Example 3 Blood Sugar Drop Test

Seven week-old SD (Sprague-Dawley) male rats were preliminarily bred for one week, and then 50 mg / 2 ml / kg of streptozotocin (STZ) was administered to the tail vein of each rat. Blood glucose levels of each rat were measured on day 3 after STZ administration. The STZ-induced diabetic rats were divided into extract administration group (6 animals) and control group (6 animals) so that the average blood glucose level was almost the same. Three rats were used as the normal group (no one administered).

Corn beard extract obtained in Example 1 was orally administered (4.07 g / kg as a dry solid) for 7 consecutive days from the third day after the STZ administration to the extract administration group. Distilled water was orally administered to the control group. The daily fluctuation of the blood glucose level of each group is shown in FIG. 2, the following facts were found.

(1) The blood glucose level of the normal group showed almost constant trend at about 110mg / dl during the test period. In contrast, the STZ-induced diabetic group had a high blood sugar level of 557 mg / dL on the first day of administration, and continued hyperglycemia after 530 mg / dL.

(2) In the hypoglycemic test using the STZ-induced diabetic rat, the blood glucose level was 370 mg / dL on the third day of the extract administration, which was significantly lower than the control group. After that, the blood glucose level was almost constant at 400 mg / dl.

(3) From the above facts, it has been found that the extract of corn whiskers has a hypoglycemic effect and is effective for the prevention and treatment of diabetes.

According to the present invention, the hypoglycemic effect of the extract of corn stubble was found. This extract can be used as a prophylactic and therapeutic agent for diabetes or as a prophylactic agent for atherosclerotic vascular disorders caused by impaired glucose tolerance. In addition, by containing the extract in the food and drink it can be expected to continue to improve or prevent diabetes in the daily diet.

Claims (8)

Blood glucose lowering agent using extract of corn whiskers as an active ingredient The hypoglycemic agent according to claim 1, wherein the corn stubble is derived from the varieties of liquids No. 12 or No. 14 corn of Shandong Shengshan, China. Food and drink having a hypoglycemic action or a hypoglycemic action containing an extract of corn whiskers. The food and drink according to claim 3, wherein the corn stubble is derived from the varieties A-C12 or A-C14 of Corn of Shandong Province, China. delete delete delete delete

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