KR100518362B1 - Control Agents in Nematode - Google Patents

Abstract

The present invention relates to nematicide control agent, powdery mildew control agent and plant disease resistant composition using one or two or more mixtures of tannin-based plant extracts. Plants used in the present invention may be made of yulpi, persimmon, hops, pine needles, medicinal herbs, amaranth, coffee, let's go, joiner, fat milk, soy sauce, rhubarb, light, and one or two or more mixtures of these extracts Excellent control against powdery mildew of nematodes and crops can be obtained. In addition, these compositions have the effect of promoting the disease resistance and crop root development of crops to promote crop growth and fruit growth, and is a composition for preventing plant diseases that can be safely used because there is little risk of human toxicity and environmental pollution.

Description

Nematode control composition {Control Agents in Nematode}

The present invention is a plant that effectively controls nematodes and powdery mildew generated in crops by extracting and filtering from yulpi, persimmon, hops, pine needles, hyacinth, sea cucumber, amaranth, coffee, let's go, joiner, fat milk, soy sauce, rhubarb and light. The present invention relates to a composition for controlling pests and plant diseases and a method of manufacturing the same.

In agriculture, chemical pesticides are used differently depending on crops and pests, and there are hundreds of species currently used. In recent years, the use of a large amount of chemical pesticides have raised the problem of resistance to pests, and toxic pesticides are detected in the agricultural products beyond the allowable value, and in many cases, the value as food is lost.

The development of plant pest control agents that are not toxic to humans and have low environmental pollution are the researchers' biggest challenges. However, the solution to this problem can be solved by the development of a microbial agent or a product using natural products, and research on this is being actively conducted. Is not made. The development of eco-friendly products with excellent control effect is still insufficient.

Root-knot nematodes have more than 700 species of host plants, with few resistant varieties and gradually becoming resistant to chemicals, which is becoming a serious problem in growing crops. Root- knot nematodes ( Meloidogyne sp.) Are phyto-parasitic nematodes that invade the roots of plants to absorb nutrients and promote complex infection of pathogenic microorganisms. Nematodes in the roots of nematodes inhibit the formation of root nodules and the growth of roots, eventually leading to plant death. In recent years, the damage of nematodes is rapidly increasing due to the increase in size of fruits and vegetables, year-round cultivation, and mass cultivation. Since chemicals cause problems such as nematode resistance and environmental pollution, biological control methods have been actively studied as an alternative to solve them. There are many types of nematodes, such as bacteria, predatory fungi, and mites. Among them, the study of predatory fungi is the most studied as a biological control method.

Powdery mildew is known from 11 genera and 80 species from 300 kinds of host plants, and the most common causes of powdery mildew are Sphaerotheca fuliginea, Sphaerotheca macularis, Sphaerotheca pannosa, Sphaerotheca humuli, Sphaerotheca phaseoli, Sphaerotheca glycines, Leveillua tauchorae, and Eearshipe ciear . . Such powdery mildew is a plant disease that occurs not only in facility cultivation but also in the open field, and has a high rate of spread, causing great damage. The onset temperature of powdery mildew is 15-25 degrees, and the humidity is 40% -85%. In open field cultivation, it usually occurs in May-June and September-November, but in facility cultivation, powdery mildew occurs all year except in summer heat. The plants affected by powdery mildew are widespread, but mainly strawberries, cucumbers, melons, melons, pumpkins, watermelons, peppers, tomatoes, peaches, apples, grapes, roses, gerberas, wolfberry and peony. In powdery mildew, symptoms vary somewhat depending on the crop, but most of the leaves, stems and fruits bloom white powdery mildew, and as the disease progresses, the diseased tissues deform rapidly as the diseased tissues necrosis by inhibiting photosynthesis and respiration. I will die. Therefore, the spread of powder in the leaves causes a great reduction in yield due to the weakening of the leaves and leaves formed on the leaves, the growth of the stems, and the inability to harvest the fruits. Powdery mildew can be controlled by spraying chemicals, but because it forms endogenous hyphae, it is difficult to completely control because it spreads in the air in the form of conidia from endogenous hyphae. Therefore, powdery mildew needs periodic control, and if the control fails, the power of the plant falls and the fruit becomes poor and the yield is greatly reduced. Recently, the control technology using microbial agents has been actively researched, but the effect is insignificant, so development of powdery mildew control agent with low human toxicity is urgently needed.

Chestnut ( Castanea crenata ) is a nut that has been used for a long time because of its excellent taste. Yulpi is an inner skin of chestnut and is a kind of industrial by-product that was removed and disposed of in the manufacture of processed food using chestnut. Recently, however, Yulpi has a skin whitening effect and is used as a component of skin care packs and is used as a natural dyeing material because of the brown pigment.

Diospyros kaki is native to Korea, China, and Japan. Persimmon is a crop that is easy to cultivate and manage due to its strong development and low damage of pests. Persimmon fruit has a strong astringent taste due to tannins. These tannins are synthesized in the leaves and transferred to the fruit. In addition, research on tannin contained in persimmon leaves has been actively conducted, and persimmon leaf tea using this has been developed as a product. Tannins contained in persimmon leaves are heat-stable and have many phenolic hydroxy groups, making them easy to gel at high concentrations and soluble in water, ethanol and methanol. Persimmon leaf tannins are known to have lipid peroxidation inhibitory and detoxifying effects, like (-)-epigallocatechin, the main component of green tea tannins.

Pine ( Pinus densiflora ) is an evergreen tree that grows well in Korea and contains ascorbic acid, carotene, vitamin K, flavonoids, tannic acid, and essential oils. Pine needles are known to be effective in neuralgia, arthritis, arteriosclerosis, scurvy, etc., and are used as tea after being dried.

Hope ( Humulus lupulus ) is mainly used as a raw material of beer. This hop contains mainly polyphenol compounds and tannin compounds. In the manufacture of beer, the tannin component in the hops combines with protein to form a precipitate, which may reduce the quality of beer.

The medicinal herb is a dry aqueous extract obtained by extracting water from the leaves and young branches of Uncaria gambir or Acacia catechu and drying them. Brown and dark brown crumbly lumps with a peculiar smell and taste very bitter and bitter. Substances are well soluble in solvents such as murcohol alcohol and acetone, and are reported to be effective in suppressing abnormal bleeding, chronic diarrhea, tonsillitis, and the like.

Acacia catechu is a legume that is used as a medicinal herb and is also called Ada. Antibacterial, antiviral, anticancer, hemostatic, burn treatment, anti-inflammatory effect is known, and the main components include tannins and pigments.

Amaranthus ( Phyllanthus emblica ) is an antibacterial and antifungal effect of antipodes and plants and has been used as a herbal medicine. It contains tannins and gallic acid as main ingredients.

Coffee, a dicotyledon, is an evergreen tree belonging to the family Agane and grown in the tropics and subtropics. Coffee is composed of caffeine, tannins, fats, minerals, etc., and about 40 kinds, including Robusta and Arabica, are used as favorite drinks and teas because of their unique aroma and taste.

The fruit of Terminalia chebula is called 'Gaza' in oriental medicine and is used for various purposes. The composition is composed of oxalic acid, terflavin, chebulic acid and gallic acid.

Joinery ( Caesalpinia sappan ) is used in diseases related to heart because of its anti-inflammatory and antibacterial activity in herbal medicine, and some studies have reported anti-cancer and whitening effect. Joiner's components include brasilin, gallic acid, gallotannin and sappanin.

Fatty oil ( Sanguisorba officinalis ) is a cucumber grass and consists of gallic acid, pedunculagin, glucogallin, and is used as an additive in herbal prescription.

The root of Polygonum cuspidatum is called Kwon Keun-Geun and is used extensively in Korean medicine. Ingredients include protocatechuic acid, oxalic acid, citric acid, cinnamic acid, gallic acid and tetrahydroxystilbene.

Rheum taguticum has antimicrobial, anti-inflammatory and tetragonal effects, and its components include physcion, gallic acid, emodin, rhein, chrysophanic acid and catechin.

Uncaria rhynchophylla consists of gallotannin, vallesiachotamine, isovallesiachotamine, vincoside lactam, strictosamide and is distributed as a herbal medicine.

As a method for controlling nematodes, physical methods such as freshwater and high temperature treatment, biological control using natural enemies and microorganisms, or chemical control such as chlorpicrine, methyl bromide, and starex are used. Chemical control is highly effective in lowering nematode density due to its high control value, but it is becoming increasingly regulated in developed countries because it causes damages such as environmental pollution, human toxicity, destruction of soil ecosystem. However, physical methods and control agents of biologics are much lower than control agents of chemicals, so the development of biologics that can replace chemicals is urgently needed.

Chemicals used to control powdery mildew include Myclobutanil, Fenarimol, Thiophanate, Benomyl, Triadimefon, Sulfur, and Chinomethionate. In addition, baking soda, clay, oils and surfactants have recently been used to control powdery mildew in crops, and non-toxic plant extracts and microorganisms have also been tried, but due to their low effectiveness, they cannot achieve realistic control effects. .

Therefore, the present invention is to provide an effective composition for controlling powdery mildew and an environmentally friendly fungal control agent by providing a composition for controlling powdery mildew and a method for producing the same by extracting and filtering a plant disease control active material from tannin-based plants.

The plants are juniper, persimmon, hops, pine needles, persimmon tree and sea hyacinth, amaranth, coffee, let's go, joiner, fat milk, soy sauce, rhubarb, and coriander, which contain large amounts of tannin compounds.

Tannin is a vegetable polyhydric alcohol that has been used as a pharmaceutical for the purpose of dissolving in water to suppress astringent action and bleeding, and has been reported to have bactericidal and antiseptic action. Tannins are largely divided into hydrolyzable tannins and catechol tannins, depending on their chemical properties. ellagic acid) and other forms of glucose are divided into gallotannin and ellagitannins. Condensed tannins are more resistant to hydrolysis than pyrogallotannin and are classified as catechin-tannin and leucoanthocanidin.

The present invention aims to enhance the growth and crop resistance of crops by using tannin-based plant extracts as an agent for controlling crop parasitic nematodes and powdery mildew.

Plant parasitic nematodes and powdery mildew are difficult to control, and high control chemicals not only cause soil contamination and poisoning toxicity, but are also limited in their use due to concerns about crop damage.

The present invention relates to a plant and its composition which can be used for efficient control of phytopathogenic powdery mildew and parasitic nematode, and not only has excellent control effect of phytosanitary nematode and powdery mildew, but also induces plant disease resistance and trigeminal development. Its aim is to provide environmentally friendly plant disease control agents that are not detrimental to crops and have little toxin toxicity.

In order to achieve the above object, the present invention, Yulepi ( Castanea crenata ), persimmon ( Diospyros kaki ), pine ( Pinus densiflora ), hop ( Humulus lupulus ), persimmon ( Uncaria gambir ), Acacia catechu , cancer Phyllanthus emblica , Coffee, Terminalia chebula , Caesalpinia sappan , Sanguisorba officinalis , Polygonum cuspidatum , Rheum taguticum , Rheum taguticum , Uncaria rhynchophylla Alternatively, the present invention provides a plant disease control composition and a method of manufacturing the same, which are effective in controlling nematodes, powdery mildew, enhancing plant disease resistance, and developing muscle roots by using one or two or more kinds of extracts by extracting hot water.

Hereinafter, the present invention will be described in more detail.

Yulepi ( Castanea crenata ), Persimmon ( Diospyros kaki ), Pine ( Pinus densiflora ), Hof ( Humulus lupulus ), Uncaria gambir , Sea cucumber Acacia catechu , Phyllanthus emblica , Coffee, Lettuce chebula , Joiner caesalpinia sappan , Sanguisorba officinalis , Polygonum cuspidatum , Rheum taguticum , Lantern ( Uncaria rhynchophylla ) Silver was prepared from one or two or more extracts or powders thereof obtained by extraction with methanol and water.

Nematodes used for nematode activity measurement in the present invention was used separately from the soil and cucumber root nodules severe nematode infection.

In the present invention, the fungus used for measuring the antimicrobial activity of fungi causing powdery mildew was used separately from the leaves and fruits of strawberries and cucumbers with severe powdery mildew.

The present invention will be described in more detail with reference to the following examples. However, these Examples are only illustrative of the present invention and the scope of the present invention is not limited thereto.

Example 1 Preparation and Validation of Water Extract of Yulpi

Yulpi was extracted with hot water to increase the yield of bioactive materials including tannins. That is, Yulpi was pulverized using a pulverizer and 1 kg of the powder was placed in a filter bag and 3L of water was added thereto for 3-4 hours. The extract was filtered or centrifuged to obtain only the extract. The extract containing the active substance was adjusted to a final dose of 1L and then tested for weakening, nematicidal effect and powdery mildew effect, respectively, as in the following experiment.

The results obtained by irrigation and foliar spraying on cucumbers and strawberries by diluting (200 times, 400 times, 600 times) the yulpi extract by concentration are shown in Table 1. Yulpi extract showed similar growth status in both crops and some roots promoted muscle development.

Yeonpi Test of Hot Water Extract Yulpi Crop and Dilution Concentration (Dilution Drainage) cucumber Strawberry 200 times 400 times 600 times 200 times 400 times 600 times Hydrothermal extract - - - - - -

-, No growth disorder

The nematicidal activity of Yulpi hot-water extract was isolated under nematode from soil and infected root nodules and added at a density of 200 per well, and treated under different concentrations, and observed under a microscope. In other words, the control group was treated only with physiological saline and the control group was added to each concentration, and the experimental group was added to each concentration at 25 ℃ incubator for 7 days while measuring the population of nematodes that survived the control and test nematode effect of the nematode effect It was confirmed. The highest mortality was obtained when the extract was treated with 200-fold dilution in saline, and the experimental group treated with 400- and 600-fold dilutions showed 65% or more mortality than the control at 7 days after treatment.

Nematode Effect of Yulpi Extract Yulpi Processing time (days) Treatment concentration (dilution drainage) 200 times 400 times 600 times Hydrothermal extract One 60 45 20 2 65 55 30 4 80 70 63 7 85 75 65

Diluted by Yulpi hot-water extract concentration in strawberry and cucumber severely affected by powdery mildew disease, and sprayed foliar on infected leaves to measure the control effect against powdery mildew, and the results are shown in Table 3. As a result of diluting Yulpi hot water extract in cucumber and strawberry by 200 times, foliar spraying resulted in excellent control of powdery mildew with 60% control rate by 10 days after foliar spraying in these crops. It can be seen that it is kept high.

Effect of Yulpi Hot Water Extract on Controlling Powdery mildew Treatment group Control rate (%) cucumber Strawberry 2 days after foliar spray 10 days after foliar spray 2 days after foliar spray 10 days after foliar spray Control 0 0 0 0 Hydrothermal extract 60 75 50 60

Example 2 Preparation and Validation of Water Extract of Persimmon

Persimmon was extracted with hot water to increase the yield of bioactive substances, including tannins. That is, the powder was pulverized using a crusher, and then 1 kg of the powder was placed in a filter bag, and 3L of water was added thereto, followed by warm extraction for 3-4 hours. The extract was filtered or centrifuged to obtain only the extract. The extract containing the active substance was adjusted to a final dose of 1L and then tested for weakening, nematicidal effect and powdery mildew effect, respectively, as in the following experiment.

Diluted persimmon extract by concentration (200 times, 400 times, 600 times), watering and foliar spraying on cucumbers and strawberries are shown in Table 4. Persimmon extract showed similar growth status in both crops and some roots promoted the development of muscle.

Weakness test of persimmon hot water extract feeling Crop and Dilution Concentration (Dilution Drainage) cucumber Strawberry 200 times 400 times 600 times 200 times 400 times 600 times Hydrothermal extract - - - - - -

-, No growth disorder

The nematicidal activity of persimmon water extract was isolated from soil and infected root nodules and injected at 200 densities per well, and treated under different concentrations and observed under a microscope. The results are shown in Table 5. In other words, the control group was treated only with physiological saline and the control group was added to each concentration, and the experimental group was added to each concentration at 25 ℃ incubator for 7 days while measuring the population of nematodes that survived the control and test nematode effect of the nematode effect It was confirmed. Diluted persimmon extract in physiological saline was 200 times the highest mortality, and the experimental group treated with dilutions 400 and 600 times showed more than 60% mortality than the control group at 7 days after treatment.

Nematode Effect of Persimmon Extract feeling Processing time (days) Treatment concentration (dilution drainage) 200 times 400 times 600 times Hydrothermal extract One 55 40 20 2 62 51 28 4 75 63 42 7 84 68 60

Diluted by Yulpi hot water extract concentration in strawberry and cucumber severely affected by powdery mildew disease and sprayed on foliar on infected leaves and stems, and the control effect on powdery mildew was measured. Yulpi hot water extract was diluted 200-fold and foliar sprayed on cucumbers and strawberries. As a result, the control rate was maintained at 60% or more until 10 days after foliar spraying in these crops. It can be seen that the control effect is high.

Effect of Persimmon Water Extracts on Controlling Powdery mildew Treatment group Control rate (%) cucumber Strawberry 2 days after foliar spray 10 days after foliar spray 2 days after foliar spray 10 days after foliar spray Control 0 0 0 0 Hydrothermal extract 55 65 45 60

Example 3 Preparation and Validation of Water Extract of Pine Needle

Pine needles were extracted with hot water to increase the yield of bioactive substances such as tannin compounds. That is, the pine needles were pulverized using a grinder, and then 1 kg of the powder was placed in a filter bag, and 3L of water was added thereto, followed by warm extraction for 3-4 hours. The extract was filtered or centrifuged to obtain only the extract. The extract containing the active substance was adjusted to a final dose of 1L and then tested for weakening, nematicidal effect and powdery mildew effect, respectively, as in the following experiment.

Dilution of pine needle extract by concentration (200 times, 400 times, 600 times) to the cucumber and strawberry through the irrigation and foliar spraying results are shown in Table 7. Pine needle extract was only effective in inhibiting growth when diluted 200 times in both crops, and when diluted more than 400 times, the growth was similar to that of the control.

Antitoxic Test of Pine Needle Extract pine needles Crop and Dilution Concentration (Dilution Drainage) cucumber Strawberry 200 times 400 times 600 times 200 times 400 times 600 times Hydrothermal extract + - - + - -

-No growth disorders; +, Growth inhibition compared to the control

The nematicidal activity of the pine needle extract was observed under a microscope after nematodes were isolated from soil and infected root nodules at 200 densities per well, and the test substances were treated by concentrations. The results are shown in Table 8. In other words, the control group was treated with physiological saline only, and the control group was treated with physiological saline only, and the experimental group was added to the extract by concentration, followed by culturing for 7 days in a 25 ° C. incubator, and measuring the population of nematodes that survived in the control and test groups. The nematode effect was confirmed. The highest mortality was obtained when the pine needle extract was diluted 200-fold in physiological saline and treated at 400- and 600-fold dilutions, which showed 75% or more mortality at 7 days after treatment.

Nematode Effect of Pine Needle Extract pine needles Processing time (days) Treatment concentration (dilution drainage) 200 times 400 times 600 times Hydrothermal extract One 73 59 45 2 78 69 53 4 87 78 67 7 97 92 75

The control effect on powdery mildew was measured by diluting pine needle hot water extracts by concentrations on strawberries and cucumbers, which were seriously affected by powdery mildew. Diluted pine leaf hot water extract by 200 times and spraying cucumber and strawberry leaves showed excellent control of powdery mildew in these crops, and it was able to observe agglomeration of powdery spores. Traces were observed.

Control Effects of Crop Water Extracts of Pine Needles on Different Crops Treatment group Control rate (%) cucumber Strawberry 2 days after foliar spray 10 days after foliar spray 2 days after foliar spray 10 days after foliar spray Control 0 0 0 0 Hydrothermal extract 56 72 54 62

Example 4 Preparation and Validation of Water Extracts of Sun Sun Medicine

The medicinal herb is extracted from Gambir or Haida and contains a large amount of bioactive substances including tannin compounds. Sub Sun medicine was pulverized using a pulverizer and then dissolved 100 grams of the powder in a small amount of ethanol and distilled water was added to the final concentration was used in the experiment to 100g / 1L. The insoluble substance of the sub-drug was filtered or centrifuged to obtain only the supernatant. The sub-drugs were identified as weak test, nematicidal effect and powdery mildew control effect as follows.

Dilution of the medicinal extract by concentration (200, 400, 600 times) by irrigation and foliar spraying on cucumbers and strawberries is shown in Table 10. The subadenosine showed similar growth to the control in both crops, and the root development was promoted in some roots.

Drug Test catechu Crop and Dilution Concentration (Dilution Drainage) cucumber Strawberry 200 times 400 times 600 times 200 times 400 times 600 times - - - - - -

-, No growth disorder

The nematicidal activity of sub-nematophytes was observed under the microscope after the nematodes were separated from soil and infected root nodules and injected at a density of 200 per well, and the test substances were treated by concentration. In other words, the control group was treated with physiological saline only after the control group and the experimental group, and the experimental group was added with a medicinal saline solution by concentration, and then cultured for 7 days in a 25 ° C. incubator, and the extracts were measured by measuring the number of nematodes surviving in the control and test groups. The nematicidal effect of was confirmed. The medicinal solution showed a mortality rate of 92% and 88% in one day when treated with 200- and 400-fold dilutions in physiological saline. All treatments showed 100% lethal effect on 7 days after treatment.

Nematode Effect of Subacute Drugs catechu Processing time (days) Treatment concentration (dilution drainage) 200 times 400 times 600 times One 92 88 82 2 95 94 89 4 100 100 98 7 100 100 100

The control effect on powdery mildew was measured by diluting the medicinal solution of strawberries and cucumbers with severe damage to powdery mildew by spraying foliar onto infected leaves and stems, and the results are shown in Table 12. As a result of dilution of the medicinal solution by 200 times and foliar spraying on cucumbers and strawberries, it was found that the powdery mildew control effect was excellent in these crops, and that the control effect was high and the sustaining effect was high in both strawberry and cucumber.

Effect of Sunshine on Controlling Powdery mildew Treatment group Control rate (%) cucumber Strawberry 2 days after foliar spray 10 days after foliar spray 2 days after foliar spray 10 days after foliar spray Control 0 0 0 0 catechu 83 95 82 93

Example 5 Preparation and Validation of Water Extracts of Hope, Amaranth, Coffee, Let's Go, Joiner, Fat Milk, Soy Sauce, Rhubarb, and Light

As in the above examples, the extracts of hops, amaranth, coffee, let's go, joiner, fat milk, soybean, rhubarb, and light were also tested for crop weakness. As a result, no growth disturbances were observed for the crops. Since no deterioration was observed, it was found that these extracts were not detrimental to crop growth.

In addition, as a result of confirming the nematicidal effect on the extracts, it was found that amaranth, coffee, light, and rhubarb showed a significantly higher nematicidal effect than the yulpina persimmon extract and similar levels of the nematicidal effect than the sub-medicinal drugs. Could. Hope, Gaza, Joiner, Fat Milk and Hojang showed similar rate of nematode lethality.

Strawberry and cucumber were severely affected by powdery mildew, hops, amaranth, coffee, let's go, joiner, fat milk, soy sauce, rhubarb, and light extracts were diluted by concentration and sprayed on infected leaves. After diluting each solution 200 times and foliar spraying on cucumbers and strawberries, it was found that amaranth, coffee, joiner, rhubarb, and light were more effective in controlling powdery mildew than hops and persimmons. .

Example 6 Mixed Preparation of Amine Medicine and Amaranth Extract and its Efficacy Verification

A mixture of sundae medicine and amaranth extract was used to carry out a phytotoxicity test, nematicidal effect, and powdery mildew control of crops. In other words, the ammonite and the sun medicine were pulverized using a grinder. 1 kg of ammonium powder was added with 6-7 L of water, followed by 2-3 hours of hot water extraction, followed by filtration or centrifugation to obtain only hot water extracts containing active ingredients such as tannin. About 200 g of powdered subline was dissolved in a small amount of ethanol, and then mixed with 1 L of ammonia hydrothermal extract. The medicinal herb and amaranth mixed solution were identified as weak test, nematicidal effect and powdery mildew control effect as follows.

The results of dilution (200 times, 400 times, 600 times) of the medicinal extracts and ammonium mixtures by concentration, and water and foliar spraying on cucumbers and strawberries are shown in Table 13. This mixed solution did not show any particular symptoms of weakening in both crops. Rather, it was found that leaf growth and growth of leaf and fruit were enhanced and glossy, and the development of muscle muscle was promoted.

Antitoxic test of sunscreen and ammonium mixture Mixed solution of sub-drug and ammonium Crop and Dilution Concentration (Dilution Drainage) cucumber Strawberry 200 times 400 times 600 times 200 times 400 times 600 times - - - - - -

-, No growth disorder

The nematicidal activity of subarachnoid and ammonium mixtures was isolated under nematode from soil and infected root nodules at 200 densities per well, and treated under different concentrations, and observed under a microscope. In other words, the control group was treated with physiological saline only, and the control group was treated with physiological saline, and the experimental group was added to the mixed solution for each concentration. The nematode effect was confirmed. In case of diluting the medicinal solution and ammonium solution by diluting 200 and 400 times in saline solution, it showed more than 96% mortality in 1 day, and 600 times more than 98% mortality in 2 days after treatment. After 1 day, all treatments had a lethal effect of 100%.

Nematode Effects of Subacute and Amaranth Subamic and Ammonite solution Processing time (days) Treatment concentration (dilution drainage) 200 times 400 times 600 times One 98 96 92 2 100 100 98 4 100 100 100 7 100 100 100

Diluted subarachnoid and amaranth mixed solution in strawberry and cucumber severely affected by powdery mildew disease by spraying foliar on infected leaves and stems to measure the control effect on powdery mildew, and the results are shown in Table 12. As a result of diluting 200-fold dilution solution of sunscreen and ammonium on leaf and spraying cucumber and strawberry, it was confirmed that powdery mildew control effect was the most excellent in these crops. .

Effect of Sunshine and Amaranth Mixture on Controlling Powdery mildew Treatment group Control rate (%) cucumber Strawberry 2 days after foliar spray 10 days after foliar spray 2 days after foliar spray 10 days after foliar spray Control 0 0 0 0 Treatment 93 100 93 99

Example 7: Mixing Preparation of Equinoxaea and Light Extract and Verification of Efficacy

Subsidiary drugs and coarse extracts were mixed and tested for the harmful effects of crops, nematode effect, and powdery mildew control. That is, the lamp and the medicine were ground using a grinder. 1 kg of powder was added with 6-7 L of water, followed by 2-3 hours of hot water extraction, followed by filtration or centrifugation to obtain only a hot water extract containing active ingredients such as tannin. About 100 g of powdered subline was dissolved in a small amount of ethanol, and then mixed with 1 L of a lamp hot water extract. Sub-medicinal drugs and light mixtures were confirmed as weak test, nematicidal effect and powdery mildew effect as shown in the following experiment, respectively.

The result of watering and foliar spraying on cucumbers and strawberries by diluting (200 times, 400 times, 600 times) the subsidiary drug and light mixture by concentration is shown in Table 16. This mixed solution did not show any particular symptoms of weakening in both crops. Rather, it was found that leaf growth and growth of leaf and fruit were enhanced and glossy, and the development of muscle muscle was promoted.

Weakness test of subarachnoid and light mixture Subtractive drug and light mixture solution Crop and Dilution Concentration (Dilution Drainage) cucumber Strawberry 200 times 400 times 600 times 200 times 400 times 600 times - - - - - -

-, No growth disorder

The nematicidal activity of subarachnoid and light mixtures was isolated under soil and infected root nodules and injected at a density of 200 per well, and the test materials were treated by concentration, and the results were observed under a microscope. In other words, the control group was treated with physiological saline only after the control and the experimental group, and the experimental group was added to the mixed solution for each concentration and then incubated for 7 days in a 25 ℃ stationary incubator, and the extract by measuring the population of nematodes surviving in the control and test The nematicidal effect of was confirmed. After dilution with 200-fold and 400-fold dilution of the sensitizer and the dilute solution in saline, the mortality rate was 94% or more in one day, and the mortality rate was 96% in the 600-fold compared to the control group at 2 days after treatment. After 1 day, all treatments had a lethal effect of 100%.

Nematode Effect of Subacute and Light Mixture Solutions Mixture solution of sublight medicine and light Processing time (days) Treatment concentration (dilution drainage) 200 times 400 times 600 times One 97 94 91 2 100 100 96 4 100 100 100 7 100 100 100

After diluting the sensitizer and light mixed solution in the strawberry and cucumber, which were seriously affected by powdery mildew, by spraying the foliar on infected leaves and stems, the control effect on powdery mildew was measured. As a result of diluting 200-fold dilution of sunscreen and light mixture on cucumber and strawberry, it was confirmed that these crops had excellent control of powdery mildew with 98% control value over 10 days after foliar spraying. High effect and excellent sustaining effect was found.

Effect of Sunshine and Light Mixtures on Control of Powdery mildew by Crops Treatment group Control rate (%) cucumber Strawberry 2 days after foliar spray 10 days after foliar spray 2 days after foliar spray 10 days after foliar spray Control 0 0 0 0 Treatment 90 100 92 98

Example 8: Mixed Product Preparation

The present invention is a mixture of extracts of yeast, persimmon, pine, hops, persimmon tree or sea hyacinth, amaranth, coffee, let's go, joiner, fat milk, soy sauce, rhubarb, crude, or two or more kinds of extracts The present invention provides a plant disease control composition and a method for producing the same, which are effective in controlling nematodes, controlling powdery mildew, enhancing plant resistance and developing muscle roots.

As in Examples 6 and 7, it can be seen that when the mixed herbal medicine is mixed with the herbal medicine as compared with the case of using the herbal medicine alone, the nematicidal effect and powdery mildew control effect are enhanced. In the present invention, the tannin-containing herbal medicines (Yulpi, persimmon, pine needles, hops, amaranth, coffee, let's go, joiner, oil, spicy sauce, rhubarb, light, etc.) having nematicidal effect and powdery mildew control effect are mixed with subsidiary drugs in a certain ratio. Since the preparation can enhance the efficacy, the mixing ratio and the kind of herbal medicine can be varied according to the use of the preparation without being limited to the above examples.

In the extraction solvent of the tannin-containing herbal medicines (Julpi, persimmon, pine needles, hops, persimmon tree or Haida (apricot), amaranth, coffee, Gaza, joiner, fat milk, soy sauce, rhubarb, crude), methanol or When extracted with ethanol, the effect of controlling nematicids and powdery mildew is increased by containing a large amount of tannin-based components and other nutrients. The herbal medicines can also be prepared extract using methanol and ethanol, and the active substance is separated even when extracted using an organic solvent such as butanol, acetone, ether, chloroform, nucleic acid, etc. It is not limited to the water extract of the example.

In addition, for use before the formalization of crops of tannin-based herbal medicines (jujupi, persimmon, pine needles, hops, persimmon tree or sea hyacinth), amaranth, coffee, let's go, joiner, fat milk, soy sauce, rhubarb, crude, etc. One or two or more powders may be prepared and sprayed directly. For convenience in farming, the powder can be transformed into various forms such as granules as granules.

The types of herbal medicines, extraction methods, extraction solvents, liquid and granular preparations, etc. may vary depending on crop characteristics and conditions of use, but are not limited thereto.

In addition, formulations for plant cultivation using tannin-based plant extracts may be mixed with a diffusing agent, a penetrant, or a surfactant to enhance the absorption and effect of the extract.

The present invention is not limited to the manufacturing process shown in the examples, and may be manufactured by other methods.

As described above, in the present invention, the tannin-containing plants (Julpi, persimmon, pine needles, hops, persimmon tree or sea hyacinth), amaranth, coffee, let's go, joiner, fat milk, soy sauce, rhubarb, crude, etc. By extracting one or two or more species with water or an organic solvent, the materials obtained by the preparation of foliar spraying and irrigation of crops are excellent in controlling powdery mildew and nematode control of crops, and promoting the growth and cropping of crops. It can improve the development and disease resistance. The optimum condition in the manufacturing process and application method is the case of using the material extracted with water, in addition to the organic solvents such as ethanol and methanol can be prepared and the formulation can be modified according to the application method.

Claims (6)

A composition for controlling parasitic nematodes, which contains a sun medicinal extract of persimmon tree or Haida Nematode control composition containing 3 wt% to 100 wt% of subarachnoid according to the formulation of plant disease control agent 3. The nematode control composition according to claim 1 or 2, wherein the formulation of the composition is a liquid for irrigation, a liquid for spraying foliar, a granular granule or powder. delete delete delete