KR100471340B1 - A new process for enhancing the efficacies of Ginseng - Google Patents
A new process for enhancing the efficacies of Ginseng Download PDFInfo
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- KR100471340B1 KR100471340B1 KR10-2003-0085983A KR20030085983A KR100471340B1 KR 100471340 B1 KR100471340 B1 KR 100471340B1 KR 20030085983 A KR20030085983 A KR 20030085983A KR 100471340 B1 KR100471340 B1 KR 100471340B1
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- ginseng
- extract
- water
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
- A23V2250/2124—Ginseng
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
본 발명은 인삼의 가공방법에 관한 것으로서, 더욱 상세하게는 인삼과, 오미자, 산사자, 산수유, 모과, 매실, 유자, 탱자, 사과, 석류 및 오렌지 중에서 선택된 1종 또는 2종 이상의 생약재를 혼합 처방함으로써 서로 상승작용을 일으켜 진세노사이드(Rg3 + Rg5)/(Rb1 + Rb2 + Rc + Rd)의 비율을 10 내지 45로 증강시키는 가공인삼 추출물 또는 가공인삼의 사포닌 분획 추출물, 및 인삼과 상기 생약재를 혼합하여 내용물이 끓는 온도에서 가열 처리하는 인삼의 가공방법에 관한 것이다.The present invention relates to a method of processing ginseng, and more specifically, by mixing one or two or more herbal medicines selected from ginseng, schizandra, hawthorn, cornus, quince, plum, citron, tanza, apple, pomegranate and orange Synergistic effect of each ginsenoside (Rg3 + Rg5) / (Rb1 + Rb2 + Rc + Rd) to enhance the ratio of 10 to 45 ginseng extract or saponin fraction extract of processed ginseng, and mixed ginseng and the herbal medicine It relates to a processing method of ginseng by heating at a temperature of boiling contents.
Description
본 발명은 인삼의 가공방법에 관한 것으로서, 더욱 상세하게는 인삼과, 오미자, 산사자, 산수유, 모과, 매실, 유자, 탱자, 사과, 석류 및 오렌지 중에서 선택된 1종 또는 2종 이상의 생약재를 혼합 처방함으로써 서로 상승작용을 일으켜 진세노사이드(Rg3 + Rg5)/(Rb1 + Rb2 + Rc + Rd)의 비율을 10 내지 45로 증강시키는 가공인삼 추출물 또는 가공인삼의 사포닌 분획 추출물, 및 인삼과 상기 생약재를 혼합하여 내용물이 끓는 온도에서 가열 처리하는 인삼의 가공방법에 관한 것이다.The present invention relates to a method of processing ginseng, and more specifically, by mixing one or two or more herbal medicines selected from ginseng, schizandra, hawthorn, cornus, quince, plum, citron, tanza, apple, pomegranate and orange Synergistic effect of each ginsenoside (Rg3 + Rg5) / (Rb1 + Rb2 + Rc + Rd) to enhance the ratio of 10 to 45 ginseng extract or saponin fraction extract of processed ginseng, and mixed ginseng and the herbal medicine It relates to a processing method of ginseng by heating at a temperature of boiling contents.
인삼은 동서양을 막론하고 가장 잘 알려져 있는 자양강장제로서 그 성분과 약리생리학적 연구가 많이 이루어져 신비한 약효가 현대 과학적 방법에 의하여 설명되고 있다. 인삼의 효능은 노화 억제, 동맥경화 예방, 고지혈증 개선, 당뇨병 개선, 고혈압 개선, 간 기능 강화, 항산화 효과, 항스트레스 효과, 면역력 증강, 혈전 개선, 뇌 기능개선, 뇌 세포 보호, 항암 효과 등 다양하여 인체의 항상성을 유지하고 모든 병리상태를 정상상태로 할 수 있다. 최근에는 홍삼에만 극미량 함유된 진세노사이드 Rg3가 혈관이완작용[J. Nat. Prod. 63, 1702(2000)], 혈소판 응집억제작용[Biol. Pharm. Bull. 21, 79(1998), Korean J. Ginseng Sci. 21, 132(1997)], 뇌신경세포 보호작용[J. Neuroscience Res. 53, 426(1998), Neuro Report 9, 226(1998)]를 나타내고, 진세노사이드 Rg3, Rg5, Rh2, Rh1은 항암 작용이 있다고 보고되었다[Jpn. J. cancer Res. 87, 357(1996), J. cancer Res. Clin. Oncol. 120, 24(1993), Anticancer Res. 17, 1067(1997), Cancer Letters 150, 41(2000), Dietary Anticarcinogenesis and Antimutagenesis 274(2000)].Ginseng is the most well-known nutritious tonic in both East and West, and its physiological and physiological studies have been conducted extensively to explain the mysterious effects by modern scientific methods. The benefits of ginseng include aging inhibition, arteriosclerosis prevention, hyperlipidemia, diabetes improvement, hypertension, liver function enhancement, antioxidant effect, antistress effect, immunity enhancement, thrombus improvement, brain function improvement, brain cell protection, anticancer effect, etc. Maintain homeostasis of human body and make all pathologies to normal state. Recently, ginsenoside Rg3 containing only trace amounts of red ginseng has vasorelaxant effect [J. Nat. Prod. 63, 1702 (2000)], platelet aggregation inhibitory activity [Biol. Pharm. Bull. 21, 79 (1998), Korean J. Ginseng Sci. 21, 132 (1997)], neuroprotective neurons [J. Neuroscience Res. 53, 426 (1998), Neuro Report 9, 226 (1998), and ginsenosides Rg3, Rg5, Rh2, Rh1 have been reported to have anticancer activity [Jpn. J. cancer Res. 87, 357 (1996), J. cancer Res. Clin. Oncol. 120, 24 (1993), Anticancer Res. 17, 1067 (1997), Cancer Letters 150, 41 (2000), Dietary Anticarcinogenesis and Antimutagenesis 274 (2000).
수삼을 장기간 보관할 수 있도록 백삼, 홍삼으로 가공하여 사용하고 있다. 홍삼은 수삼을 가열 처리하여 제조되는 것으로서 백삼에 비하여 우수한 약효를 가지고 있는 것으로 널리 알려져 있다. 특히, 홍삼에는 수삼, 백삼에 함유되지 않은 진세노사이드 Rg3, Rg5, Rk1, Rh2, Rh1, 말톨 등 미량 성분이 존재하여 약효가 증가하는 것으로 알려졌다. 가정에서 수삼이나 백삼에서 홍삼 엑기스를 제조할 수 있는 방법이 고안되어 그 제조를 위한 기계도 이미 알려진 바 있다[대한민국 특허 공개번호 제 10-2001-19628호]. 이처럼 홍삼에 미량 함유된 성분을 강화하여 약효를 증강시키는 방법들이 연구되어 지고 있다.The fresh ginseng is processed into white and red ginseng for long-term storage. Red ginseng is manufactured by heat-treating ginseng and is widely known to have excellent efficacy compared to white ginseng. In particular, red ginseng has been known to increase the drug efficacy due to the presence of trace components such as ginsenosides Rg3, Rg5, Rk1, Rh2, Rh1, maltol not contained in ginseng and white ginseng. A method for producing red ginseng extract from fresh ginseng or white ginseng has been devised at home, and a machine for manufacturing the same has already been known [Korean Patent Publication No. 10-2001-19628]. As such, methods for enhancing drug efficacy by strengthening components contained in red ginseng have been studied.
특히, 그 중에서도 최근 들어서는 소위 선삼(仙蔘)이라 하여, 인삼을 소량의 물 존재하에 홍삼 제조 온도보다 훨씬 높은 온도 120 내지 180 ℃에서 가열 처리하여 홍삼에 미량 함유되었던 성분들의 함량이 크게 증가하고 새로운 성분이 생성되어 약효가 크게 증가된다는 사실도 알려졌다[J. Natural Products 63, 1702, 2000, 대한민국 특허 등록 제 192678호]. 그러나, 이 방법은 제조시간이 많이 걸리거나, 고압 가열기 등 특수장비가 필요하고 특히, 통상의 가공 온도 보다 고온으로 가열 처리해야 하므로 대량 제조할 때 인삼이 탄화되는 경우가 발생하는 등 제조공정의 운영상 어려움이 많다. Particularly, among these, so called ginseng in recent years, the ginseng is heat-treated in the presence of a small amount of water at a temperature of 120-180 ° C., which is much higher than the red ginseng manufacturing temperature, thereby greatly increasing the content of the components contained in the red ginseng. It is also known that the ingredients are produced and the drug efficacy is greatly increased [J. Natural Products 63, 1702, 2000, Korean Patent Registration No. 192678]. However, this method requires a lot of manufacturing time or requires special equipment such as a high-pressure heater, and in particular, the ginseng is carbonized during mass production because it needs to be heated to a higher temperature than the normal processing temperature. There are many difficulties.
이에, 본 발명자는 인삼의 약효를 증강시킬 수 있는 과학적 연구를 수행해 오던 중 고온에서 인삼을 가열처리하여 찌는 가공방법과는 달리, 내용물이 끓는 온도에서 인삼에 특정 생약재를 혼합 처방하여 가열 탕약을 추출함으로써 진세노사이드(Rg3 + Rg5)/(Rb1 + Rb2 + Rc + Rd)의 비율을 10 내지 45, 대개는 20 내지 45로 증강시켜 기존인삼 보다 혈관이완작용이 65 내지 97 배 증강하는 등의 놀라운 약효를 발휘하는 가공인삼 추출물을 개발함으로써 본 발명을 완성하게 되었다.Thus, the present inventors have been doing scientific research to enhance the efficacy of ginseng, unlike the processing method of steaming the ginseng by heating at high temperature, unlike the processing method of boiling the contents at the boiling temperature by mixing the prescribed herbal medicines to extract the heated decoction By increasing the ratio of ginsenosides (Rg3 + Rg5) / (Rb1 + Rb2 + Rc + Rd) to 10 to 45, usually 20 to 45, the vasorelaxation effect is increased by 65 to 97 times compared to conventional ginseng. The present invention has been completed by developing a processed ginseng extract exhibiting efficacy.
따라서, 본 방법은 기존인삼 보다 월등히 약효가 증강된 진세노사이드(Rg3 + Rg5)/(Rb1 + Rb2 + Rc + Rd)의 비율이 10 ∼ 45인 가공인삼 추출물 또는 이의 사포닌 분획 추출물을 제공하는 데 그 목적이 있다.Therefore, the present method provides a processed ginseng extract or a saponin fraction extract thereof having a ratio of ginsenosides (Rg3 + Rg5) / (Rb1 + Rb2 + Rc + Rd) that is significantly enhanced than conventional ginseng in a ratio of 10 to 45. The purpose is.
또한, 본 발명은 인삼에 오미자, 산사자, 산수유, 모과, 매실, 유자, 탱자, 사과, 석류, 오렌지와 같은 산미를 갖는 생약재와 함께 내용물이 끓는 온도에서 가열 처리함으로써 프로토파낙사디올계 사포닌이 변화되어 진세노사이드 Rg3, Rg5, Rk1이 다량 생성되며 프로토파낙사트리올계 사포닌은 진세노 사이드 Rg2, F4, Rh1 등으로 변화되는 인삼의 새로운 가공방법을 제공하는데 그 목적이 있다. In addition, the present invention changes the protopanaxadiol-based saponins by heating at a temperature of boiling contents with herbal medicines having acidity such as Schisandra chinensis, Sansa, Cornus, Chinese quince, plum, citron, tanza, apple, pomegranate, orange in ginseng The ginsenosides Rg3, Rg5, Rk1 is produced in a large amount, and the protoparnaxatriol-based saponins aim to provide a new processing method of ginseng that is changed to ginsenosides Rg2, F4, Rh1 and the like.
또한, 본 발명에 따른 가공인삼 추출물 또는 사포닌 분획 추출물은 그대로 농축액, 희석액 또는 음료조성물에 응용하거나, 이를 분말화하여 정제, 캅셀제, 환제, 과립제 등으로, 또는 과자류에 첨가한 형태의 건강식품 또는 의약품을 제공하는 데 또 다른 목적이 있다. In addition, the processed ginseng extract or saponin fraction extract according to the present invention is applied to a concentrate, diluent or beverage composition as it is, or powdered to a tablet, capsules, pills, granules, or the like added to confectionary or health food or pharmaceutical form There is another purpose to provide.
본 발명은 진세노사이드(Rg3 + Rg5)/(Rb1 + Rb2 + Rc + Rd)의 비율이 10 ∼ 45인 가공인삼 추출물 또는 이의 사포닌 분획 추출물을 그 특징으로 한다.The present invention is characterized by the ginseng extract (Rg3 + Rg5) / (Rb1 + Rb2 + Rc + Rd) ratio of 10 to 45 processed ginseng extract or saponin fraction extract thereof.
또한, 본 발명은 인삼에 오미자, 산사자, 산수유, 모과, 매실, 유자, 탱자, 사과, 석류 및 오렌지 중에서 선택된 1종 또는 2종 이상의 생약재를 혼합하고 내용물이 끓는 온도에서 가열 처리하는 새로운 인삼의 가공방법을 또 다른 특징으로 한다.In addition, the present invention is the processing of a new ginseng mixed with ginseng, hawthorn, hawthorn, cornus, quince, plum, citron, tanza, apple, pomegranate and orange selected from ginseng and two or more herbal medicines and heated at the temperature of boiling contents The method is another feature.
또한, 본 발명은 상기 가공인삼 추출물 또는 이의 사포닌 분획 추출물이 심혈관 질환, 발기부전, 뇌 신경세포 관련 질환 예방 및 개선, 그리고 항암 효과 등의 우수한 기능성 효능을 지님으로써 이를 유효성분으로 함유하는 다양한 제형의 의약품 및 건강식품을 포함한다.In addition, the present invention ginseng extract or saponin fraction extract thereof has excellent functional efficacy, such as cardiovascular disease, erectile dysfunction, cerebral nerve cell-related diseases prevention and improvement, and anti-cancer effect of various formulations containing it as an active ingredient Medicines and health food.
먼저, 본 발명에 따른 인삼의 가공방법을 상세히 설명하면 다음과 같다.First, the processing method of ginseng according to the present invention will be described in detail.
본 발명은 인삼 100 중량부에 대하여 오미자, 산사자, 산수유, 모과, 매실, 유자, 탱자, 사과, 석류, 오렌지 등의 산미를 내는 생약재 10 내지 100 중량부를 혼합하여 사용한다. 상기 혼합물을 물 4 ∼ 10 배량에서 1 ∼ 6시간 가열한 후 실온으로 냉각한 다음, 여과하여 여액을 농축시켜 엑기스나 분말로 제조할 수 있다. 이때, 사용하는 물의 양은 추출 초기에는 많은 양(예를 들면 7 ∼ 10 배량)을 넣어 추출 후기에는 4 ∼ 5 배량이 되도록 가열함으로써 추출조 내의 온도를 높게 할 수 있어 반응이 촉진된다. The present invention is used by mixing 10 to 100 parts by weight of herbal medicines, such as Schisandra chinensis, lioness, cornus, Chinese quince, plum, citron, tanza, apple, pomegranate, orange, etc. to 100 parts by weight of ginseng. The mixture may be heated in 4 to 10 times of water for 1 to 6 hours, cooled to room temperature, filtered, and the filtrate is concentrated to prepare an extract or powder. At this time, the amount of water to be used can increase the temperature in the extraction tank by heating a large amount (for example, 7 to 10 times) at the beginning of the extraction, and heating it so as to be 4 to 5 times at the late stage of the extraction, thereby promoting the reaction.
또한, 상기 혼합물을 가열 시 온도는 70 내지 120 ℃, 바람직하게는 내용물이 끓는 온도에서 탕약 추출하여도 충분하며, 산업현장에서 통상적으로 사용하고 있는 추출장치인 가압 용출조를 사용하면 복잡한 추가의 설비가 필요하지 않고서도 쉽게 상업적으로 대량 제조할 수 있다.In addition, when the mixture is heated, the temperature is 70 to 120 ℃, preferably it is sufficient to extract the medicinal extract at the boiling temperature of the contents, a complicated additional equipment by using a pressurized elution tank which is commonly used in industrial sites Easily commercially manufactured in bulk without the need for
가열시간은 1 ∼ 6시간, 바람직하게는 3 ∼ 5시간 동안 수행하며, 이 범위를 벗어나면 수득율에 별로 변동이 없으면서 오히려 진세노이드 Rg3, Rg5가 분해될 수 있다.The heating time is carried out for 1 to 6 hours, preferably 3 to 5 hours, and outside this range, the ginsenoids Rg3 and Rg5 may be decomposed without any change in yield.
이렇게 얻어진 가공인삼 추출물을 그대로 사용하여도 본 발명의 목적을 달성할 수 있으나, 상기 추출물을 물을 증발시키고 초기 물의 양에 대하여 1.2 ∼ 2배의 에탄올 또는 메탄올로 1 ∼ 3시간 가열 추출하는 단계를 추가로 시행하는 과정을 거칠 수도 있다. 이런 과정을 거치는 것은 특히 사포닌 용출 효율을 높히는 공정에 적용하는 경우 바람직한 결과를 얻을 수가 있다.Although the object of the present invention can be achieved by using the obtained ginseng extract as it is, the step of extracting the extract by heating the extract with 1.2 to 2 times ethanol or methanol for 1 to 3 hours with respect to the amount of initial water Additional steps may be taken. Going through this process can produce desirable results, especially when applied to a process that increases the saponin elution efficiency.
또한, 상기와 같이 추출한 가공인삼 추출물은 비교적 인삼과 혼합 처방된 생약재에서의 비약효성 불순물 등이 함유되어 있으므로 이를 좀더 정제하기 위해서는 예를 들어 디클로로메탄 등으로 추출하고 수층을 수포화 부탄올 등으로 여러 번 추출하여 합하고 물로 세척하고 부탄올 추출액을 감압 농축하여 메탄올에 녹이는 과정을 추가로 거침으로써 불필요한 추출 불순물 등을 적절히 제거한 다음 순도를 증강시킨 사포닌 분획 추출물을 얻을 수 있는 바, 이러한 사포닌 분획 추출물을 사용하는 경우 더욱 고순도의 제품으로 사용할 수 있다.In addition, the processed ginseng extract extracted as described above contains non-pharmaceutical impurities in herbal medicines mixed with ginseng relatively, for further purification, for example, extracted with dichloromethane and the aqueous layer several times with saturated butanol. Extracted, combined, washed with water, butanol extract is concentrated under reduced pressure to dissolve in methanol to further remove unnecessary impurities, and then saponin fraction extract with enhanced purity can be obtained. It can be used as a higher purity product.
한편, 본 발명에 사용하는 인삼은 수삼, 백삼, 미삼, 홍삼, 인삼엽, 화기삼, 삼칠인삼 또는 죽절인삼이 바람직하다.Meanwhile, the ginseng used in the present invention is preferably ginseng, white ginseng, rice ginseng, red ginseng, ginseng leaf, hwagi ginseng, samchil ginseng or bamboo shoot ginseng.
또한, 본 발명에서 인삼 가공 시 함께 사용되는 생약재들은 산미를 내는 것으로서, 오미자, 산사자, 산수유, 모과, 매실, 유자, 탱자, 사과, 석류, 오렌지, 중에서 1종 또는 2종 이상을 혼합하여 인삼과 함께 혼합 처방으로 사용한다. 본 발명에 사용되는 오미자는 여러 가지 리그난 성분이 함유되어 있어 간의 손상을 예방하고 간 손상의 회복을 촉진하며 간 기능정상화, 담즙분비항진 효과, 항박테리아, 항산화제, 소화촉진제로서 폐, 간, 신장을 위해 사용되고 있다. 피로회복, 스트레스 저항력증강, 시력피로, 인식능력증가에도 유효한 연구 결과들이 많다. 산사자는 위액 분비촉진, 항균작용, 지속성 혈압강하작용으로 건위, 소화, 정장약으로 사용하고 최근에는 관상동맥확장작용, 콜레스테롤 강하 효과가 있어 혈액순환 개선제로 널리 사용되고 있다. 산수유는 이뇨작용, 일시적 혈압강하작용, 항균작용, 항히스타민작용이 있어 자양강장 수렴 지혈 목적으로 사용되고 있다. 유자는 향신료로, 탱자는 방향성 건위제로서 식품 및 한약재로 사용되고 있지만, 이들의 과육 속에는 구연산 등 식물 유기산이 다량 함유되어 있어 식초대용으로 사용되거나 생선요리의 비린내를 제거하는 향신료로 사용되고 있다. 매실은 항균작용, 소화액 분비촉진, 청량 지갈의 작용이 있어 청량성 수렴약, 진해 거담, 해열, 구충작용이 있다. 모과는 신진대사를 도와주고 소화 효소의 분비를 촉진시켜주는 효과를 발휘한다. 사과는 비타민과 미네날이 풍부하며, 특히 섬유질이 많아서 정장효과와 진정작용가 뛰어난다. 석류는 옛부터 강장제로 알려져 왔으며, 특히 고혈압과 동맥경화 예방에 좋은 효과를 나타낸다. 또한 설사, 이질, 복통, 대하증 등에 마시면 구충제의 작용도 한다. 오렌지는 비타민 C 의 함유량이 많아 피로회복, 피부미용에 효과가 있다. In addition, the herbal medicines used together in the ginseng processing in the present invention is to produce acidity, ginseng family by mixing one or two or more of Schisandra chinensis, Sansa, Cornus, Chinese quince, plum, citron, tanza, apple, pomegranate, orange, Use as a mixed prescription together. Schizandra chinensis used in the present invention contains various lignans, which prevents liver damage, promotes recovery of liver damage, normalizes liver function, promotes bile secretion, antibacterial, antioxidant, and digestive stimulants such as lung, liver and kidney. It is being used for There are many studies that are effective for fatigue recovery, stress resistance, vision fatigue, and cognitive ability. Sansa is used as a gastric juice, antibacterial action, persistent blood pressure lowering action, in the stomach, digestion, and formal medicine. Recently, it is widely used as a blood circulation improving agent because of its coronary artery dilation and cholesterol lowering effect. Cornus milk has diuretic effect, temporary blood pressure lowering effect, antibacterial effect and antihistamine effect, and is used for nourishing tonic convergent hemostasis. Citron is used as a spice and tanza is used as a food and medicinal herb as aromatic fragrance. However, the pulp contains a large amount of organic organic acids such as citric acid and is used as a vinegar substitute or as a spice to remove fishy fish. Plum has the action of antibacterial action, digestive juice secretion, and cold jigal action, it has a cooling astringent drug, Jinhae expectoration, fever, antiparasitic action. Chinese quince helps metabolism and stimulates the release of digestive enzymes. Apples are rich in vitamins and minerals. Especially, they are high in fiber, so they have a good suitability and calming effect. Pomegranate has long been known as a tonic, and has a particularly good effect on the prevention of hypertension and atherosclerosis. In addition, diarrhea, dysentery, abdominal pain, drooping and drinking also acts as a repellent. Orange is rich in vitamin C, which is effective for fatigue and skin beauty.
이와 같이, 본 발명에서는 종래와는 달리, 인삼과 오미자, 산사자, 산수유, 모과, 매실, 유자, 탱자, 사과, 석류, 오렌지 등의 생약재를 혼합 처방하여 가열 추출함으로써 기존에 인삼 단일성분을 고온 처리한 경우와는 달리 복합 약재가 서로 상승작용을 일으켜 진세노사이드(Rg3 + Rg5)/(Rb1 + Rb2 + Rc + Rd)의 비율이 10 ∼ 45로 크게 증강된 효과를 나타내며, 고혈압, 동맥경화, 혈전, 발기부전, 뇌졸증, 기억력 장애 예방 및 치료, 항암 효과 및 항암제 부작용을 경감시키는 등의 우수한 약효를 지님을 확인할 수 있었다.Thus, in the present invention, unlike conventional, ginseng and Schizandra chinensis, hawthorn, cornus, quince, plum, citron, tanza, apple, pomegranate, orange, etc. by mixing and prescribing herbal medicines such as apples, pomegranates, oranges, etc. Unlike one case, the combined medicinal herbs synergize with each other, resulting in a significant increase in the ratio of ginsenosides (Rg3 + Rg5) / (Rb1 + Rb2 + Rc + Rd) to 10-45, hypertension, arteriosclerosis, Thrombosis, erectile dysfunction, stroke, memory disorders prevention and treatment, anti-cancer effects and anti-cancer drug side effects were found to have excellent efficacy.
한편, 본 발명에 따른 가공인삼 추출물 또는 이의 사포닌 분획 추출물을 유효성분으로 하는 의약품 또는 건강식품을 포함하는 바, 가공인삼 추출물의 의약품, 건강식품 또는 식품첨가제로의 적용은 통상의 제형화나 첨가방법에 의해 다양하게 적용될 수 있다.On the other hand, the processed ginseng extract or saponin fraction extract according to the present invention includes a drug or a health food containing the active ingredient bar, the application of the processed ginseng extract as a medicine, health food or food additives in a conventional formulation or method of addition It can be applied in various ways.
예컨대, 의약품으로 제조시에는 경구 투여 제제에 통상적으로 사용되는 첨가제, 유화제, 활탁제, 점착제, 감미제, 방향제 등을 이용하여 정제, 경질캅셀제, 연질캅셀제, 환제, 과립제, 액제와 같은 경구 투여 제제, 주사제 등으로 제조가 가능하다.For example, when prepared as a pharmaceutical, oral dosage preparations such as tablets, hard capsules, soft capsules, pills, granules, liquids, etc., using additives, emulsifiers, suspending agents, pressure-sensitive adhesives, sweetening agents, fragrances, etc., which are commonly used in oral dosage forms, It can be manufactured by injection.
본 발명의 가공인삼 추출물 또는 이의 사포닌 분획 추출물은 사용되어지는 원료 생약이 의약품 또는 건강식품으로 사용할 수 있으며 함께 가열 처리하더라도 무독성으로 안전하게 섭취할 수 있다. The processed ginseng extract of the present invention or the saponin fraction extract thereof may be used as a pharmaceutical raw material used as a medicine or a health food and may be safely ingested without toxicity even when heated together.
본 발명에 의한 가공인삼 추출물 또는 이의 사포닌 분획 추출물은 성인 60 kg 체중을 기준으로 100 내지 1,000 ㎎씩 하루 2 ∼ 4회 복용하는 것이 바람직하다. The processed ginseng extract or saponin fraction extract thereof according to the present invention is preferably administered 2 to 4 times a day from 100 to 1,000 mg based on 60 kg of adult body weight.
한편, 식품첨가제로 제조시에는 음료, 정제, 과립제, 캅셀제, 환제, 껌, 과자류 등의 식품소재에 본 발명의 가공인삼 추출물 또는 사포닌 분획 추출물을 포함시켜 제조할 수 있다.On the other hand, when manufacturing as a food additive may be prepared by including the processed ginseng extract or saponin fraction extract of the present invention in food materials such as beverages, tablets, granules, capsules, pills, gum, confectionery.
이러한 본 발명에 따른 가공인삼 추출물을 위에 예시한 각종 제형으로 제조하여 다양한 방법으로 복용하는 경우 발기부전 개선, 혈액 순환 개선, 피로회복, 고혈압, 동맥 경화, 항 혈전, 뇌졸증 및 뇌 기능 개선 등에 효과가 있고, 또한 항암 효과뿐만 아니라 항암제 부작용을 경감시키는 효능도 지니고 있다. When the processed ginseng extract according to the present invention is prepared in various formulations exemplified above, it is effective in improving erectile dysfunction, improving blood circulation, fatigue, hypertension, arteriosclerosis, antithrombosis, stroke and brain function when taken in various ways. In addition, it has the effect of reducing the anti-cancer effects as well as anti-cancer effects.
이하, 본 발명은 다음 실시예에 의거하여 더욱 상세하게 설명하겠는 바, 본 발명이 이에 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail based on the following examples, but the present invention is not limited thereto.
실시예 1 Example 1
백삼 2.5 Kg과 오미자 1.5 Kg에 물 40 ℓ를 넣고 가압 추출장치에서 4시간 동안 가열하여 끓인 후(내부 온도 120 ℃) 물 20 ℓ를 증류하여 제거하고 에탄올 48 ℓ를 넣고 2시간 가열하여 추출하고 실온으로 냉각한 후 여과하였다(1차 여액). 잔사에 물 16 ℓ와 에탄올 48 ℓ를 넣고 3시간 가열추출하고 냉각한 후 여과한 여액과 1차 여액을 합하고 추출물 용량이 1/3 될 때까지 농축하고 분무 건조하여 가공인삼 추출물 분말을 얻었다.Add 40 ℓ of water to 2.5 Kg of white ginseng and 1.5 Kg of Schisandra chinensis and heat it in a pressure extractor for 4 hours, boil it (internal temperature 120 ℃), distill out 20 ℓ of water, add 48 ethanol and extract for 2 hours, It was cooled to and filtered (primary filtrate). 16 ℓ of water and 48 ethanol were added to the residue, followed by heating, extraction and cooling for 3 hours. The filtered filtrate and the primary filtrate were combined, concentrated to an extract volume of 1/3, and spray dried to obtain a processed ginseng extract powder.
실시예 2 Example 2
백삼 2.5 Kg과 산사자 1.5 Kg을 상기 실시예 1과 동일하게 처리하여 가공인삼추출물 분말을 얻었다.2.5 g g of white ginseng and 1.5 Kg of mountain lion were treated in the same manner as in Example 1 to obtain a processed ginseng extract powder.
실시예 3Example 3
미삼 50 g과 오미자 30 g에 물 700 ㎖를 넣고 4시간 동안 가열하여 끓인 후 실온으로 냉각한 후 여과하고 여액을 감압 농축하여 가공인삼 농축물을 얻었다.To 50 g of ginseng and 30 g of Schizandra chinensis, 700 ml of water was added, boiled for 4 hours, boiled, cooled to room temperature, filtered, and the filtrate was concentrated under reduced pressure to obtain a processed ginseng concentrate.
실시예 4Example 4
홍삼 20 g과 오미자 15 g에 물 300 ㎖을 넣고 약 100 ℃에서 3시간 가열한 후 실온으로 냉각하고, 여과하여 여액을 감압 농축하여 가공인삼 분말을 얻었다.300 g of water was added to 20 g of red ginseng and 15 g of Schisandra chinensis, heated at about 100 ° C. for 3 hours, cooled to room temperature, filtered, and the filtrate was concentrated under reduced pressure to obtain a processed ginseng powder.
실시예 5Example 5
수삼 25 g을 잘게 썰어서 산사자 20 g과 합하고 물 200 ㎖를 넣고 가열하여 4시간 끓인 후 실온으로 냉각, 여과하고 여액을 감압 농축하여 가공인삼 분말을 얻었다.25 g of fresh ginseng was finely chopped, combined with 20 g of mountain lions, 200 ml of water was added thereto, heated to boil for 4 hours, cooled to room temperature, filtered, and the filtrate was concentrated under reduced pressure to obtain a processed ginseng powder.
실시예 6Example 6
백삼 20 g과 모과 25 g에 물 400 ㎖를 넣고 상기 실시예 3과 같이 수행하여 가공인삼 추출액을 제조하였다.400 g of water was added to 20 g of white ginseng and 25 g of Chinese quince, and the processed ginseng extract was prepared in the same manner as in Example 3.
실시예 7Example 7
백삼 20 g과 매실 과육 건조품 15 g에 물 300 ㎖를 넣고 상기 실시예 3과 같이 수행하여 가공인삼 농축물을 제조하였다.300 g of water was added to 20 g of white ginseng and 15 g of plum flesh dried product to prepare a processed ginseng concentrate as in Example 3.
실시예 8Example 8
백삼 15 g과 유자 과육 15 g에 물 200 ㎖를 넣고 상기 실시예 3과 같이 수행하여 가공인삼 농축물을 제조하였다.15 g of white ginseng and 15 g of citron pulp were put in 200 ml of water to carry out as in Example 3 to prepare a processed ginseng concentrate.
실시예 9Example 9
홍삼 15 g과 탱자 20 g에 물 300 ㎖를 넣고 상기 실시예 3과 같이 수행하여 가공인삼 농축물을 제조하였다.15 g of red ginseng and 20 g of tanza were added to 300 ml of water to prepare a processed ginseng concentrate as in Example 3.
실시예 10 Example 10
화기삼 20 g과 산수유 20 g, 죽절인삼 20 g과 산사자 20 g, 산칠인삼 15 g, 건조한 인삼엽 20 g과 오미자 15 g에 물 350 ㎖를 각각 넣고 4시간 가열하여 끓이고 물을 증류하여 제거하고 에탄올 400 ㎖를 넣고 2시간 가열 환류시키고 실온으로 냉각, 여과하여 여액을 감압 농축하여 가공인삼 농축물을 제조하였다. 20 g of fire ginseng, 20 g of cornus ginseng, 20 g of bamboo shoot ginseng, 20 g of mountain lion, 20 g of wild ginseng, 15 g of dried ginseng leaf, 20 g of dried ginseng leaf and 15 g of Schizandra chinensis were added 350 ml of water and boiled for 4 hours, and water was distilled off. 400 ml of the mixture was heated to reflux for 2 hours, cooled to room temperature, filtered, and the filtrate was concentrated under reduced pressure to prepare a processed ginseng concentrate.
실시예 11Example 11
백삼 10 g과 산사자 10 g에 물 200 ㎖를 넣고 1시간 가열하여 끓이고 물 100 ㎖를 증발시킨 후 뚜껑을 닫고 3시간 가열한 후 물을 증발시켜 제거하였다. 여기에 메탄올 400 ㎖를 가하여 1시간 가열 추출하고 냉각하여 여과하여 가공인삼 추출물을 얻었다. 10 g of white ginseng and 10 g of mountain lion were put in 200 ml of water, heated for 1 hour to boil, 100 ml of water was evaporated, the lid was closed, heated for 3 hours, and water was evaporated and removed. 400 ml of methanol was added thereto, and the mixture was heated and extracted for 1 hour, cooled, and filtered to obtain a processed ginseng extract.
실시예 12Example 12
백삼 10 g과 오미자 10 g에 물 200 ㎖를 넣고 1시간 가열하여 끓이고 물 100 ㎖를 증발시킨 후 뚜껑을 닫고 3시간 가열한 후 물을 증발시켜 제거하였다. 여기에 메탄올 400 ㎖를 가하여 1시간 가열 추출하고 냉각하여 여과하여 가공인삼 추출물을 얻었다. 200 g of water was added to 10 g of white ginseng and 10 g of Schisandra chinensis and heated for 1 hour to boil. After evaporating 100 ml of water, the lid was closed and heated for 3 hours. 400 ml of methanol was added thereto, and the mixture was heated and extracted for 1 hour, cooled, and filtered to obtain a processed ginseng extract.
실시예 13Example 13
백삼 10 g과 산수유 10 g에 물 200 ㎖를 넣고 1시간 가열하여 끓이고 물 100 ㎖를 증발시킨 후 뚜껑을 닫고 3시간 가열한 후 물을 증발시켜 제거하였다. 여기에 메탄올 400 ㎖를 가하여 1시간 가열 추출하고 냉각하여 여과하여 가공인삼 추출물을 얻었다. 200 g of white ginseng and 10 g of cornus oil were added and boiled for 1 hour, boiled and evaporated 100 ml of water, the lid was closed, heated for 3 hours, and water was removed by evaporation. 400 ml of methanol was added thereto, and the mixture was heated and extracted for 1 hour, cooled, and filtered to obtain a processed ginseng extract.
실시예 14Example 14
백삼 10 g과 모과 10 g에 물 200 ㎖를 넣고 1시간 가열하여 끓이고 물 100 ㎖를 증발시킨 후 뚜껑을 닫고 3시간 가열한 후 물을 증발시켜 제거하였다. 여기에 메탄올 400 ㎖를 가하여 1시간 가열 추출하고 냉각하여 여과하여 가공인삼 추출물을 얻었다. 10 g of white ginseng and 10 g of Chinese quince were put in 200 ml of water, heated for 1 hour to boil, 100 ml of water was evaporated, the lid was closed and heated for 3 hours, and then water was evaporated and removed. 400 ml of methanol was added thereto, and the mixture was heated and extracted for 1 hour, cooled, and filtered to obtain a processed ginseng extract.
실시예 15Example 15
백삼 10 g과 매실 과육 10 g에 물 200 ㎖를 넣고 1시간 가열하여 끓이고 물 100 ㎖를 증발시킨 후 뚜껑을 닫고 3시간 가열한 후 물을 증발시켜 제거하였다. 여기에 메탄올 400 ㎖를 가하여 1시간 가열 추출하고 냉각하여 여과하여 가공인삼 추출물을 얻었다.10 g of white ginseng and 10 g of plum pulp were added to 200 ml of water, heated for 1 hour to boil, 100 ml of water was evaporated, the lid was closed, heated for 3 hours, and water was evaporated to remove it. 400 ml of methanol was added thereto, and the mixture was heated and extracted for 1 hour, cooled, and filtered to obtain a processed ginseng extract.
실시예 16Example 16
백삼 10 g과 유자 과육 10 g에 물 200 ㎖를 넣고 1시간 가열하여 끓이고 물 100 ㎖를 증발시킨 후 뚜껑을 닫고 3시간 가열한 후 물을 증발시켜 제거하였다. 여기에 메탄올 400 ㎖를 가하여 1시간 가열 추출하고 냉각하여 여과하여 가공인삼 추출물을 얻었다. 10 g of white ginseng and 10 g of citron pulp were put in 200 ml of water, heated for 1 hour to boil, 100 ml of water was evaporated, the lid was closed, heated for 3 hours, and water was evaporated and removed. 400 ml of methanol was added thereto, and the mixture was heated and extracted for 1 hour, cooled, and filtered to obtain a processed ginseng extract.
실시예 17Example 17
백삼 10 g과 탱자 10 g에 물 200 ㎖를 넣고 1시간 가열하여 끓이고 물 100 ㎖를 증발시킨 후 뚜껑을 닫고 3시간 가열한 후 물을 증발시켜 제거하였다. 여기에 메탄올 400 ㎖를 가하여 1시간 가열 추출하고 냉각하여 여과하여 가공인삼 추출물을 얻었다. 200 g of water was added to 10 g of white ginseng and 10 g of tanza, heated to boil for 1 hour, boiled and evaporated 100 ml of water, the lid was closed and heated for 3 hours, and then evaporated to remove water. 400 ml of methanol was added thereto, and the mixture was heated and extracted for 1 hour, cooled, and filtered to obtain a processed ginseng extract.
실시예 18Example 18
홍삼 10 g과 오미자 10 g에 물 200 ㎖를 넣고 1시간 가열하여 끓이고 물 100 ㎖를 증발시킨 후 뚜껑을 닫고 3시간 가열한 후 물을 증발시켜 제거하였다. 여기에 메탄올 400 ㎖를 가하여 1시간 가열 추출하고 냉각하여 여과하여 가공인삼 추출물을 얻었다. 10 g of red ginseng and 10 g of Schizandra chinensis were added 200 ml of water, heated for 1 hour to boil, 100 ml of water was evaporated, the lid was closed, heated for 3 hours, and water was evaporated to remove. 400 ml of methanol was added thereto, and the mixture was heated and extracted for 1 hour, cooled, and filtered to obtain a processed ginseng extract.
실시예 19Example 19
상기 실시예 1에서 얻은 가공인삼 추출물 50 g을 물 350 mℓ에 녹이고 디클로로메탄 350 ㎖씩 2회 추출하고 수층을 수포화 부탄올 350 ㎖씩으로 3회 추출하여 합하고 물 350 ㎖로 세척한 다음 부탄올 추출액을 감압 농축하여 인삼 사포닌 분획 추출물을 얻었다.50 g of the processed ginseng extract obtained in Example 1 was dissolved in 350 ml of water, extracted twice with 350 ml of dichloromethane, and the aqueous layer was extracted three times with 350 ml of saturated butanol, washed with 350 ml of water, and the butanol extract was decompressed. Concentrated to obtain ginseng saponin fraction extract.
실시예 20Example 20
상기 실시예 2에서 얻은 가공인삼 추출물 50 g을 물 350 ㎖에 녹이고 디클로로메탄 350 ㎖씩 2회 추출하고 수층을 수포화 부탄올 350 ㎖씩으로 3회 추출하여 합하고 물 350 ㎖로 세척한 다음 부탄올 추출액을 감압 농축하여 인삼 사포닌 분획 추출물을 얻었다.50 g of the processed ginseng extract obtained in Example 2 was dissolved in 350 ml of water, extracted twice with 350 ml of dichloromethane, the aqueous layer was extracted three times with 350 ml of saturated butanol, and the mixture was washed with 350 ml of water, and the butanol extract was decompressed. Concentrated to obtain ginseng saponin fraction extract.
실시예 21Example 21
상기 실시예 11에서 얻은 가공인삼 추출물 5.1 g을 물 50 ㎖에 녹이고 디클로로메탄 50 ㎖씩 2회 추출하고 수층을 수포화 부탄올 50 ㎖씩으로 3회 추출하여 합하고 물 50 ㎖로 세척한 다음 부탄올 추출액을 감압 농축하여 인삼 사포닌 분획 추출물을 얻었다.5.1 g of the processed ginseng extract obtained in Example 11 was dissolved in 50 ml of water, 50 ml of dichloromethane was extracted twice, and the aqueous layers were extracted three times with 50 ml of saturated butanol, washed with 50 ml of water, and the butanol extract was decompressed. Concentrated to obtain ginseng saponin fraction extract.
실시예 22Example 22
상기 실시예 12에서 얻은 가공인삼 추출물 5.4 g을 물 50 ㎖에 녹이고 디클로로메탄 50 ㎖씩 2회 추출하고 수층을 수포화 부탄올 50 ㎖씩으로 3회 추출하여 합하고 물 50 ㎖로 세척한 다음 부탄올 추출액을 감압 농축하여 인삼 사포닌 분획 추출물을 얻었다.5.4 g of the processed ginseng extract obtained in Example 12 was dissolved in 50 ml of water, extracted twice with 50 ml of dichloromethane, the aqueous layers were extracted three times with 50 ml of saturated butanol, washed with 50 ml of water, and the butanol extract was decompressed. Concentrated to obtain ginseng saponin fraction extract.
실시예 23Example 23
상기 실시예 13에서 얻은 가공인삼 추출물 5 g을 물 50 ㎖에 녹이고 디클로로메탄 50 ㎖씩 2회 추출하고 수층을 수포화 부탄올 50 ㎖씩으로 3회 추출하여 합하고 물 50 ㎖로 세척한 다음 부탄올 추출액을 감압 농축하여 인삼 사포닌 분획 추출물을 얻었다.5 g of the processed ginseng extract obtained in Example 13 was dissolved in 50 ml of water, 50 ml of dichloromethane was extracted twice, and the aqueous layers were extracted three times with 50 ml of saturated butanol, washed with 50 ml of water, and the butanol extract was decompressed. Concentrated to obtain ginseng saponin fraction extract.
실시예 24Example 24
상기 실시예 14에서 얻은 가공인삼 추출물 5.5 g을 물 50 ㎖에 녹이고 디클로로메탄 50 ㎖씩 2회 추출하고 수층을 수포화 부탄올 50 ㎖씩으로 3회 추출하여 합하고 물 50 ㎖로 세척한 다음 부탄올 추출액을 감압 농축하여 인삼 사포닌 분획 추출물을 얻었다.5.5 g of the processed ginseng extract obtained in Example 14 was dissolved in 50 ml of water, extracted twice with 50 ml of dichloromethane, the aqueous layer was extracted three times with 50 ml of saturated butanol, washed with 50 ml of water, and the butanol extract was decompressed. Concentrated to obtain ginseng saponin fraction extract.
실시예 25Example 25
상기 실시예 15에서 얻은 가공인삼 추출물 4.8 g을 물 50 ㎖에 녹이고 디클로로메탄 50 ㎖씩 2회 추출하고 수층을 수포화 부탄올 50 ㎖씩으로 3회 추출하여 합하고 물 50 ㎖로 세척한 다음 부탄올 추출액을 감압 농축하여 인삼 사포닌 분획 추출물을 얻었다.4.8 g of the processed ginseng extract obtained in Example 15 was dissolved in 50 ml of water, extracted twice with 50 ml of dichloromethane, the aqueous layers were extracted three times with 50 ml of saturated butanol, washed with 50 ml of water, and the butanol extract was decompressed. Concentrated to obtain ginseng saponin fraction extract.
실시예 26Example 26
상기 실시예 16에서 얻은 가공인삼 추출물 4.9 g을 물 50 ㎖에 녹이고 디클로로메탄 50 ㎖씩 2회 추출하고 수층을 수포화 부탄올 50 ㎖씩으로 3회 추출하여 합하고 물 50 ㎖로 세척한 다음 부탄올 추출액을 감압 농축하여 인삼 사포닌 분획 추출물을 얻었다.4.9 g of the processed ginseng extract obtained in Example 16 was dissolved in 50 ml of water, extracted twice with 50 ml of dichloromethane, the aqueous layers were extracted three times with 50 ml of saturated butanol, washed with 50 ml of water, and the butanol extract was decompressed. Concentrated to obtain ginseng saponin fraction extract.
실시예 27Example 27
상기 실시예 17에서 얻은 가공인삼 추출물 5.1 g을 물 50 ㎖에 녹이고 디클로로메탄 50 ㎖씩 2회 추출하고 수층을 수포화 부탄올 50 ㎖씩으로 3회 추출하여 합하고 물 50 ㎖로 세척한 다음 부탄올 추출액을 감압 농축하여 인삼 사포닌 분획 추출물을 얻었다.5.1 g of the processed ginseng extract obtained in Example 17 was dissolved in 50 ml of water, 50 ml of dichloromethane was extracted twice, and the aqueous layers were extracted three times with 50 ml of saturated butanol, washed with 50 ml of water, and the butanol extract was decompressed. Concentrated to obtain ginseng saponin fraction extract.
실시예 28Example 28
상기 실시예 18에서 얻은 가공인삼 추출물 5.2 g을 물 50 ㎖에 녹이고 디클로로메탄 50 ㎖씩 2회 추출하고 수층을 수포화 부탄올 50 ㎖씩으로 3회 추출하여 합하고 물 50 ㎖로 세척한 다음 부탄올 추출액을 감압 농축하여 인삼 사포닌 분획 추출물을 얻었다.5.2 g of the processed ginseng extract obtained in Example 18 was dissolved in 50 ml of water, extracted twice with 50 ml of dichloromethane, and the aqueous layers were extracted three times with 50 ml of saturated butanol, washed with 50 ml of water, and the butanol extract was decompressed. Concentrated to obtain ginseng saponin fraction extract.
실시예 29Example 29
백삼 15 g, 산사자 5 g 및 오미자 5 g 에 물 250 mℓ를 넣고 5시간 동안 가열하여 끓인 후 실온으로 냉각하고 여과하여 여액을 감압농축하여 가공인삼추출물을 얻었다. 250 g of water was added to 15 g of white ginseng, 5 g of hawthorn, and 5 g of Schisandra chinensis. The mixture was heated and boiled for 5 hours, cooled to room temperature, filtered, and the filtrate was concentrated under reduced pressure to obtain a processed ginseng extract.
실시예 30Example 30
백삼 15 g, 오미자 3g, 산사자 3g 및 산수유 4 g 에 물 250 mℓ를 넣고 5시간 동안 가열하여 끓인 후 실온으로 냉각하고 여과하여 여액을 감압농축하여 가공인삼추출물을 얻었다. 250 g of water was added to 15 g of white ginseng, 3 g of Schisandra chinensis, 3 g of Sansa, and 4 g of cornus oil, heated for 5 hours to boil, cooled to room temperature, filtered, and the filtrate was concentrated under reduced pressure to obtain a processed ginseng extract.
참고실시예 1: 정제 제조Reference Example 1: Tablet Preparation
상기 실시예 1에서 제조한 가공인삼 추출물 분말 500 g과 결정성 셀룰로오스 196 g, 스테아린산마그네슘 4 g을 잘 혼합하고 통상의 방법에 따라 타정하여 700 mg 정제를 제조하였다.500 g of the processed ginseng extract powder prepared in Example 1, 196 g of crystalline cellulose, and 4 g of magnesium stearate were mixed well and tableted according to a conventional method to prepare 700 mg tablets.
참고실시예 2: 정제 제조Reference Example 2: Tablet Preparation
상기 실시예 2에서 제조한 가공인삼 추출물 500 g, 결정성 셀룰로오스 196 g, 스테아린산마그네슘 4 g을 잘 혼합하여 통상의 방법에 따라 타정하여 700 mg정제를 제조하였다.500 g of the processed ginseng extract prepared in Example 2, 196 g of crystalline cellulose, and 4 g of magnesium stearate were mixed well and tableted according to a conventional method to prepare 700 mg tablets.
참고실시예 3: 경질캅셀제 제조Reference Example 3: Preparation of Hard Capsule
상기 실시예 1에서 제조한 가공인삼 추출물 분말 100 g, 결정성 셀룰로오스 95 g, 산화규소 5 g을 잘 혼합하고 통상의 방법에 따라 400 mg 경질캅셀제를 제조하였다.100 g of the processed ginseng extract powder prepared in Example 1, 95 g of crystalline cellulose, and 5 g of silicon oxide were mixed well to prepare a 400 mg hard capsule according to a conventional method.
참고실시예 4: 연질캅셀제 제조Reference Example 4: Manufacture of soft capsule
상기 실시예 1에서 제조한 가공인삼 추출물 분말 200 g, 대두유 280 g, 레시친 15 g, 황납 5 g을 균질하게 혼합하고 통상의 방법에 따라 500 mg 연질캅셀을 제조하였다.200 g of the processed ginseng extract powder prepared in Example 1, 280 g of soybean oil, 15 g of lecithin and 5 g of lead were homogeneously mixed and 500 mg of a soft capsule was prepared according to a conventional method.
참고실시예 5: 과립제 제조Reference Example 5: Preparation of Granules
상기 실시예 2에서 제조한 가공인삼 추출물 500 g, 옥수수전분 196 g, 스테아린산마그네슘 4 g을 잘 혼합하고 통상의 방법에 따라 과립제를 제조하였다.500 g of the processed ginseng extract prepared in Example 2, 196 g of corn starch, and 4 g of magnesium stearate were mixed well and granules were prepared according to a conventional method.
참고실시예 6: 환제의 제조Reference Example 6: Preparation of Pills
상기 실시예 2에서 제조한 가공인삼 추출물 300 g, 옥수수전분 100 g, 결정성 셀룰로오스 50 g, 벌꿀 150 g을 잘 혼합하여 통상의 방법에 따라 100 mg 환제를 제조하였다.300 g of the processed ginseng extract prepared in Example 2, 100 g of corn starch, 50 g of crystalline cellulose, and 150 g of honey were mixed well to prepare 100 mg pills according to a conventional method.
참고실시예 7: 액제의 제조Reference Example 7 Preparation of Liquid
상기 실시예 1에서 제조한 가공인삼 추출물 100 g, 홍삼추출물 농축액(인삼사포닌 140 mg/g) 20 g, 동충하초 추출물 분말(2.5 : 1로 농축한 것) 100 g, 대추농축액(55 브릭스) 50 g, 비타민 B1 염산염 2 g, 비타민 B2 1 g, 비타민 B6 염산염 2 g, 설탕 200 g에 정제수를 가하여 용해시키고 2 ℓ로 맞추어 액제를 제조하였다.100 g of the processed ginseng extract prepared in Example 1, 20 g of red ginseng extract concentrate (140 g / g of ginseng saponin), 100 g of Cordyceps sinensis extract powder (concentrated to 2.5: 1), 50 g of jujube concentrate (55 brix) Purified water was added to 2 g of vitamin B1 hydrochloride, 1 g of vitamin B2, 2 g of vitamin B6 hydrochloride, and 200 g of sugar to prepare a liquid formulation.
참고실시예 8 : 음료 제품 제조Reference Example 8: Beverage Product Preparation
상기 실시예 1에서 제조한 가공인삼 추출물 10 g, 구연산 1 g, 아라비아검 1 g, 설탕 5 g을 물에 희석하여 통상의 방법으로 음료를 제조하되 총 100 ㎖로 맞춘 후 95 ℃에서 15초간 살균, 냉각하여 드링크 타입의 음료 제품을 제조하였다.10 g of the processed ginseng extract prepared in Example 1, 1 g of citric acid, 1 g of Arabian gum, 5 g of sugar were diluted in water to prepare a beverage in a conventional manner, but after adjusting to 100 ml in total, sterilizing for 15 seconds at 95 ℃. After cooling, a drink type beverage product was prepared.
비교예 1Comparative Example 1
홍삼 2 Kg, 백삼 5 Kg, 오미자 5 Kg, 산사자 5 Kg을 각각 별도로 상기 실시예 3과 같이 수행하여 추출물 분말을 제조하였다.2 Kg of red ginseng, 5 Kg of white ginseng, 5 Kg of Schisandra chinensis and 5 Kg of Sansa were separately prepared as in Example 3 to prepare an extract powder.
비교예 2Comparative Example 2
백삼 10 g에 물 200 ㎖를 넣고 1시간 가열하여 끓이고 물 100 ㎖를 증발시킨 후 뚜껑을 닫고 3시간 가열한 후 물을 증발시켜 제거하였다. 여기에 메탄올 400 ㎖를 가하여 1시간 가열 추출하고 냉각하여 여과하여 가공인삼 추출물을 얻었다. 200 g of water was added to 10 g of white ginseng, and the mixture was heated and boiled for 1 hour, 100 ml of water was evaporated, the lid was closed, heated for 3 hours, and then evaporated to remove water. 400 ml of methanol was added thereto, and the mixture was heated and extracted for 1 hour, cooled, and filtered to obtain a processed ginseng extract.
비교예 3Comparative Example 3
홍삼 10 g에 물 200 ㎖를 넣고 1시간 가열하여 끓이고 물 100 ㎖를 증발시킨 후 뚜껑을 닫고 3시간 가열한 후 물을 증발시켜 제거하였다. 여기에 메탄올 400 ㎖를 가하여 1시간 가열 추출하고 냉각하여 여과하여 가공인삼 추출물을 얻었다. 200 g of water was added to 10 g of red ginseng, and the mixture was heated and boiled for 1 hour, 100 ml of water was evaporated, the lid was closed, heated for 3 hours, and the water was evaporated and removed. 400 ml of methanol was added thereto, and the mixture was heated and extracted for 1 hour, cooled, and filtered to obtain a processed ginseng extract.
비교예 4Comparative Example 4
상기 비교예 2에서 얻은 가공인삼 추출물 3.4 g을 물 50 ㎖에 녹이고 디클로로메탄 50 ㎖씩 2회 추출하고 수층을 수포화 부탄올 50 ㎖씩으로 3회 추출하여 합하고 물 50 ㎖로 세척한 다음 부탄올 추출액을 감압 농축하여 인삼 사포닌 분획 추출물을 얻었다.3.4 g of the processed ginseng extract obtained in Comparative Example 2 was dissolved in 50 ml of water, 50 ml of dichloromethane was extracted twice, and the aqueous layer was extracted three times with 50 ml of saturated butanol, washed with 50 ml of water, and the butanol extract was decompressed. Concentrated to obtain ginseng saponin fraction extract.
비교예 5Comparative Example 5
상기 비교예 3에서 얻은 가공인삼 추출물 3.4 g을 물 50 ㎖에 녹이고 디클로로메탄 50 ㎖씩 2회 추출하고 수층을 수포화 부탄올 50 ㎖씩으로 3회 추출하여 합하고 물 50 ㎖로 세척한 다음 부탄올 추출액을 감압 농축하여 인삼 사포닌 분획 추출물을 얻었다.3.4 g of the processed ginseng extract obtained in Comparative Example 3 was dissolved in 50 ml of water, 50 ml of dichloromethane was extracted twice, and the aqueous layers were extracted three times with 50 ml of saturated butanol, washed with 50 ml of water, and the butanol extract was decompressed. Concentrated to obtain ginseng saponin fraction extract.
비교예 6: 선삼의 제조(대한민국 특허 등록 제 192678호)Comparative Example 6: Preparation of ginseng (Korean Patent Registration No. 192678)
40 ㎖ 스테인레스 용기에 백삼 5 g과 물 5 ㎖를 가한 후 가압멸균기에 넣고 밀폐하여 120 ℃에서 2시간 동안 가열하였다. 가열이 끝난 가공인삼 5 g을 취하여 메탄올 100 ㎖씩으로 3회 추출하고 농축시킨 후에 물에 현탁시키고 에테르 100 ㎖씩으로 3회 추출하였다. 남은 수층을 부탄올 100 ㎖씩으로 3회 추출한 후에 부탄올 분획을 농축시켜 인삼 사포닌 분획 추출물을 얻었다.5 g of white ginseng and 5 ml of water were added to a 40 ml stainless container, which was then placed in an autoclave and heated at 120 ° C. for 2 hours. 5 g of the processed ginseng was heated, extracted three times with 100 ml of methanol, concentrated, suspended in water, and extracted three times with 100 ml of ether. The remaining aqueous layer was extracted three times with 100 ml of butanol, and the butanol fraction was concentrated to obtain a ginseng saponin fraction extract.
비교예 7: 선삼의 제조(대한민국 특허 등록 제 192678호)Comparative Example 7: Preparation of ginseng (Korean Patent Registration No. 192678)
40 ㎖ 스테인레스 용기에 백삼 5 g과 물 5 ㎖를 가한 후 가압멸균기에 넣고 밀폐하여 130 ℃에서 2시간 동안 가열하였다. 가열이 끝난 가공인삼 5 g을 취하여 메탄올 100 ㎖씩으로 3회 추출하고 농축시킨 후에 물에 현탁시키고 에테르 100 ㎖씩으로 3회 추출하였다. 남은 수층을 부탄올 100 ㎖씩으로 3회 추출한 후에 부탄올 분획을 농축시켜 인삼 사포닌 분획 추출물을 얻었다.5 g of white ginseng and 5 ml of water were added to a 40 ml stainless container, which was then placed in an autoclave and heated at 130 ° C. for 2 hours. 5 g of the processed ginseng was heated, extracted three times with 100 ml of methanol, concentrated, suspended in water, and extracted three times with 100 ml of ether. The remaining aqueous layer was extracted three times with 100 ml of butanol, and the butanol fraction was concentrated to obtain a ginseng saponin fraction extract.
비교예 8 Comparative Example 8
홍삼 5 g을 메탄올 100 mℓ씩 3회 3시간 가열추출하고 농축한 후 물 100 mℓ에 현탁시키고 에테르 100 mℓ씩 3회 추출하였다. 수층을 부탄올 100 mℓ씩 3회 추출하여 합하고 농축하여 홍삼의 사포닌 분획 추출물을 얻었다.5 g of red ginseng was extracted with 100 ml of methanol three times for 3 hours, concentrated, suspended in 100 ml of water, and extracted three times with 100 ml of ether. The aqueous layer was extracted three times with 100 ml of butanol, combined and concentrated to obtain a saponin fraction extract of red ginseng.
참고비교예 1 Reference Example 1
상기 비교예 1에서 제조한 백삼 추출물 350 g, 오미자 추출물 210 g, 결정성 셀룰로오스 136 g, 스테아린산마그네슘 4 g을 잘 혼합하고 통상의 방법으로 타정하여 700 mg 정제를 제조하였다.350 g of white ginseng extract prepared in Comparative Example 1, 210 g of Schisandra chinensis extract, 136 g of crystalline cellulose, and 4 g of magnesium stearate were mixed well and compressed into a conventional method to prepare 700 mg tablets.
참고비교예 2Comparative Example 2
상기 비교예 1에서 제조한 백삼 추출물 350 g, 산사자 추출물 210 g, 결정성 셀룰로오스 136 g, 스테아린산마그네슘 4 g을 잘 혼합하고 통상의 방법에 따라 타정하여 700 mg 정제를 제조하였다.350 g of white ginseng extract prepared in Comparative Example 1, 210 g of mountain lion extract, 136 g of crystalline cellulose, and 4 g of magnesium stearate were mixed well and tableted according to a conventional method to prepare 700 mg tablets.
참고비교예 3Comparative Example 3
상기 비교예 1에서 제조한 홍삼 추출물 350 g, 결정성 셀룰로오스 246 g, 옥수수전분 100 g, 스테아린산 4 g을 잘 혼합하고 통상의 방법에 따라 타정하여 700 mg 정제를 제조하였다.350 g of red ginseng extract prepared in Comparative Example 1, 246 g of crystalline cellulose, 100 g of corn starch, and 4 g of stearic acid were mixed well and tableted according to a conventional method to prepare 700 mg tablets.
실험예 1 Experimental Example 1
상기 실시예 21 ∼ 28 및 비교예 4 ∼ 5에서 얻은 사포닌 분획 추출물을 박층크로마토그래피법으로 분석하였다(전개 용매 : 클로르포름: 메탄올: 물 = 15 : 10 : 2.5, 발색제 : 10% 황산을 분무한 후 가열)[도 1 및 도 2]. 또한, 고속액체크로마토그래피(HPLC)는 Capcell pak MG C18(4.6 ×250 mm), 검출 203 nm, 용출용매는 (A) 10% 아세토니트릴/물, (B) 90% 아세토니트릴/물을 시간대 별로 비율차이를 두어 실시하여 그 결과를 도 3에 나타내었다.The saponin fraction extracts obtained in Examples 21 to 28 and Comparative Examples 4 to 5 were analyzed by thin layer chromatography (developing solvent: chloroform: methanol: water = 15: 10: 2.5, colorant: 10% sulfuric acid sprayed) Post heating) [FIGS. 1 and 2]. In addition, high-performance liquid chromatography (HPLC) is Capcell pak MG C18 (4.6 x 250 mm), detection 203 nm, elution solvent (A) 10% acetonitrile / water, (B) 90% acetonitrile / water It carried out with the ratio difference, and the result is shown in FIG.
도 1과 도 2에서 보는 바와 같이, 비교예 4 및 5는 진세노사이드의 변화가 관찰되지 않았다. 그러나, 실시예 21 내지 28에서는 진세노사이드들이 대부분 변화하여 극성이 적은 진세노사이드들이 많이 생성된 것을 확인하였다. As shown in Figures 1 and 2, in Comparative Examples 4 and 5 there was no change in ginsenosides. However, in Examples 21 to 28, it was confirmed that ginsenosides were mostly changed to generate a lot of ginsenosides with less polarity.
도 3에서 보는 바와 같이, 비교예 4의 진세노사이드들은(PG-Q로 표시) 대부분 변화하여(GS-1 표시는 실시예 22, GC-1 표시는 실시예 21) 진세노사이드 Rg3, Rg5, Rk1, Rg2, Rh1, F4로 전환되었음을 확인하였다. 피크 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13은 각각 진세노사이드 Rg1, Re, Rb1, Rc, Rb2, Rd, Rg2, Rh1, F4, (20S)-Rg3, (20R)-Rg3, Rk1, Rg5를 나타낸다. As shown in FIG. 3, ginsenosides of Comparative Example 4 (denoted by PG-Q) were mostly changed (GS-1 in Example 22, GC-1 in Example 21), and ginsenosides Rg3 and Rg5. , Rk1, Rg2, Rh1, it was confirmed that the conversion to F4. Peaks 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 are ginsenosides Rg1, Re, Rb1, Rc, Rb2, Rd, Rg2, Rh1, F4, ( 20S) -Rg3, (20R) -Rg3, Rk1, and Rg5 are shown.
상기 실시예 19 ∼ 28 및 비교예 4 ∼ 7에서 얻은 사포닌 분획 추출물을 고속액체크로마토그래피로 분석하여 진세노사이드(Rg3 + Rg5)/(Rb1 + Rb2 + Rc + Rd)의 비율을 각 성분의 상대 피크면적을 기준으로 분석하여 그 결과를 다음 표 1에 나타내었다. The saponin fraction extracts obtained in Examples 19 to 28 and Comparative Examples 4 to 7 were analyzed by high performance liquid chromatography, and the ratio of ginsenosides (Rg3 + Rg5) / (Rb1 + Rb2 + Rc + Rd) was compared with each other. Analysis based on the peak area is shown in Table 1 below.
상기 표 1에서 나타낸 바와 같이, 본 발명에 따른 가공인삼 추출물의 진세노사이드(Rg3 + Rg5)/(Rb1 + Rb2 + Rc + Rd)의 비율은 10 ∼ 45로서 선행기술(대한민국 특허등록번호 제 192678호)의 비교예 6과 비교예 7에서 1.03 ∼ 2.43인 것에 비하여 월등히 우수하다. 또한, 실시예 19 및 20의 경우에는 실시예 21 및 22와 동일한 생약재로 가공된 인삼이지만 대량 가공하더라도 역시 진세노사이드(Rg3 + Rg5)/(Rb1 + Rb2 + Rc + Rd)의 비율이 매우 증가되었음을 알 수 있다.As shown in Table 1, the ratio of ginsenoside (Rg3 + Rg5) / (Rb1 + Rb2 + Rc + Rd) of the processed ginseng extract according to the present invention is 10 to 45 as the prior art (Korean Patent Registration No. 192678). It is much superior to 1.03-2.43 in Comparative Example 6 and Comparative Example 7 of No.). In addition, in the case of Examples 19 and 20, but the ginseng processed with the same herbal medicines as Examples 21 and 22, but also in the mass processing, the ratio of ginsenosides (Rg3 + Rg5) / (Rb1 + Rb2 + Rc + Rd) is also greatly increased It can be seen that.
실험예 2: 발기부전증 개선효능 비교Experimental Example 2: Comparison of Efficacy of Erectile Dysfunction
발기부전증을 갖고 있는 45 ∼ 60세 남성 40명을 8명씩 5군으로 나누고, 상기 참고실시예 1, 참고실시예 2, 참고비교예 1, 참고비교예 2 및 참고비교예 3에서 제조한 정제를 1회 2정씩 1일 3회 30일간 복용시키고, 발기부전증 개선효과를 자각증상과 문진으로 평가하여 그 결과를 다음 표 2에 나타내었다.40 male 45-60 years old with erectile dysfunction were divided into 5 groups of 8 persons, and the tablets prepared in Reference Example 1, Reference Example 2, Reference Comparative Example 1, Reference Comparative Example 2 and Reference Comparative Example 3 were prepared. Two tablets once a day, three times a day for 30 days, and the effects of improving erectile dysfunction were evaluated by subjective symptoms and questionnaires, and the results are shown in Table 2 below.
상기 표 2에 나타낸 바와 같이, 본 발명에 따른 가공인삼 추출물 참고실시예 1 및 2는 87.5%의 발기부전개선효과를 보였으며, 참고비교예 1 및 2는 발기부전 개선효과가 미미하였다. 문헌 상 홍삼도 발기부전 개선효과가 있다고 보고되어 있으나, 본 발명의 가공인삼 추출물이 홍삼(참고비교예 3)보다 훨씬 우수한 효과를 나타내었다.As shown in Table 2, Example 1 and 2 of the processed ginseng extract according to the present invention showed an improvement in erectile dysfunction of 87.5%, and Comparative Examples 1 and 2 were insignificant. Although red ginseng has been reported in the literature to improve the erectile dysfunction, the processed ginseng extract of the present invention showed a much better effect than red ginseng (Comparative Example 3).
실험예 3: 말초 혈액순환 개선작용Experimental Example 3: Peripheral Blood Circulation Improvement
경미한 신경장애를 갖는 49 ∼ 60세, 남성 당뇨병 환자 40명을 8명씩 5개 군으로 나누고, 상기 참고실시예 1, 참고실시예 2, 참고비교예 1 및 참고비교예 2에서 제조한 정제를 6정씩을 복용시키고 복용 전, 복용 후 45분, 복용 후 90분에 Laser Doppler Flowmetry 장치로 환자들의 우측 발에 대한 혈류 흐름을 측정하였다. 위약군은 같은 량의 옥수수전분으로 만든 정제를 복용시켰다. 그 결과를 다음 표 3에 나타내었다.Forty-nine years old with mild neuropathy, 40 male diabetic patients were divided into five groups of eight, and the tablets prepared in Reference Example 1, Reference Example 2, Reference Comparative Example 1 and Reference Comparative Example 2 The blood flow to the right foot of the patients was measured with a Laser Doppler Flowmetry device before and after 45 minutes and 90 minutes after the dose. The placebo group took tablets made from the same amount of corn starch. The results are shown in Table 3 below.
상기 표 3에 나타낸 바와 같이, 복용 후 90분에서의 참고실시예 1, 참고실시예 2는 각각 51.2, 68.2% 혈류 증가를 보이는 반면에, 참고비교예 1, 참고비교예 2와 같이 인삼 추출물에 오미자 추출물이나 산사자 추출물을 단순히 혼합하여 투여한 군은 15, 19%의 혈류 증가를 나타냄으로써, 본 발명에 따른 가공인삼 추출물이 혈류 장애를 받는 당뇨병 환자의 발에서 혈류를 증강시킴을 알 수 있었다. As shown in Table 3, Reference Example 1 and Reference Example 2 showed an increase in blood flow of 51.2 and 68.2%, respectively, at 90 minutes after administration, while the Ginseng extracts as in Comparative Example 1 and Reference Example 2, respectively. The group administered simply by mixing Schisandra chinensis extract or Sansa extract showed an increase in blood flow of 15 and 19%.
실험예 4: 혈관이완작용의 비교Experimental Example 4: Comparison of Vascular Relaxation
체중 260 ∼ 280 g의 스프라그도우리계 래트의 흉부대동맥을 적출하고 내피세포를 손상시키지 않게 2 ∼ 3 mm 길이로 대동맥환을 만들었다. 크랩스탄산완충액(mM; NaCl 118, KCl 4.7, CaCl2 2.5, MgSO4 1.2, KH2PO4 1.2, 포도당 11.0, 95% O2 + 5% CO2, 37 ℃) 20 ㎖가 채워진 장기 챔버에 현수하고 등척성 장력의 기록장치에 연결하였다. 대동맥환을 2 g 휴지장력으로 60분간 평행시켰다. 페닐에프린 30 μM을 가하여 안정된 수축이 나타난 것을 확인하고, 아세틸콜린 1 μM을 가하여 내피세포가 존재하는 것을 확인하였다. 대동맥환을 45분간 3회 세척하고 다시 페닐에프린으로 수축시키고 시료를 누적적으로 가하고 혈관이완작용을 관측하였다[도 4]. 사용한 시료는 상기 실시예 21, 실시예 22 및 비교예 4에서 얻은 사포닌 분획 추출물을 사용하였다.The thoracic aorta of Sprague-Dawley rats weighing 260-280 g was extracted and aortic rings were made 2-3 mm long so as not to damage endothelial cells. In a long-term chamber filled with 20 ml of crabstannic acid buffer (mM; NaCl 118, KCl 4.7, CaCl 2 2.5, MgSO 4 1.2, KH 2 PO 4 1.2, glucose 11.0, 95% O 2 + 5% CO 2 , 37 ° C) Suspended and connected to an isometric tension recorder. The aortic ring was paralleled for 60 minutes with 2 g resting tension. 30 μM of phenylephrine was added to confirm that stable contraction was observed, and 1 μM of acetylcholine was added to confirm the presence of endothelial cells. The aortic ring was washed three times for 45 minutes, and then contracted again with phenylephrine, and the sample was added cumulatively to observe vasorelaxation (FIG. 4). As the sample used, saponin fraction extracts obtained in Example 21, Example 22 and Comparative Example 4 were used.
도 4에서 나타낸 바와 같이, 본 발명에 따른 가공인삼 사포닌 분획 추출물의 혈관이완작용이 비교예 4의 사포닌 분획 추출물(- ▲- 표시) 보다 실시예 21의 사포닌 분획 추출물(- ●- 표시)이 65배, 실시예 22의 사포닌 분획 추출물(- ○- 표시)은 97배 강력한 효과를 나타내었다. 본 발명에 의한 가공인삼은 선행기술[대한민국 특허 등록 제 192678호 및 J. Natural Products 63, 1702, 2000]에 의한 가공인삼(32배 약효 증가)보다 훨씬 우수한 것을 알 수 있었다.As shown in Figure 4, the vasorelaxant effect of the processed ginseng saponin fraction extract according to the present invention is 65 saponin fraction extract (-▲-mark) of Comparative Example 4 than the saponin fraction extract (-▲-mark) of 65 Pear, saponin fraction extract of Example 22 (-○-indicated) showed a 97-fold powerful effect. Processed ginseng according to the present invention was found to be much superior to the processed ginseng (32 times increase in efficacy) according to the prior art (Korean Patent Registration No. 192678 and J. Natural Products 63, 1702, 2000).
실험예 5: 고혈압에 대한 효과Experimental Example 5: Effect on hypertension
45 ∼ 60세 남성 고혈압 환자 21명을 11명, 10명 두군으로 나누고, 한군에는 상기 참고실시예 2에서 제조한 정제를, 다른 한 군에는 상기 참고비교예 2에서 제조한 정제를 1일 3회 1회 2정씩을 30일간 복용시키고 혈압의 변화를 측정하여 다음 표 4에 나타내었다.Twenty-one patients with hypertension of 45 to 60 years old were divided into two groups of 11 and 10 patients, one group of tablets prepared in Reference Example 2, and the other group of tablets prepared in Reference Comparative Example 2 three times a day. Take 2 tablets once every 30 days and measure the change in blood pressure are shown in Table 4 below.
상기 표 4에 나타낸 바와 같이, 본 발명에 따른 가공인삼 추출물을 1개월 간 복용했을 때 고혈압자의 수축기 혈압을 강하시키는 비율은 90.9%이었고 변화된 혈압은 -14 ∼ -36 mmHg이었으며, 기존인삼 추출물에 산사자 추출물을 합한 것을 복용한 군은 10명 중 2명(20%)만 혈압이 낮아지고 80%는 거의 변화가 없었다. 본 발명에 따른 가공인삼 추출물은 고혈압자의 수축기 혈압을 강하시키는 효과가 우수하였다. As shown in Table 4, when the processed ginseng extract according to the present invention was taken for one month, the rate of dropping systolic blood pressure of hypertensive patients was 90.9%, and the changed blood pressure was -14 to -36 mmHg. In the group taking the combined extracts, only 2 out of 10 (20%) had lowered blood pressure and 80% had little change. The processed ginseng extract according to the present invention was excellent in reducing the systolic blood pressure of high blood pressure.
실험예 6: 혈소판 응집 억제 효과Experimental Example 6: Platelet aggregation inhibitory effect
건강한 남자의 상박정맥에서 채혈하고(항응고제는 citric acid-citrate-dextrose 15% 사용), 120 g에서 15분간 원심분리하여 얻은 혈소판이 풍부한 혈장 500 g에서 10분간 원심분리하여 혈소판을 얻었다. 2억개 혈소판/㎖로 현탁시켜 Washed platelet를 얻었다. 문헌[고려인삼학회지 21, 132 (1997)]에 나타난 대로 콜라겐으로 유도되는 혈소판 응집반응에 대한 가공인삼 추출물의 효과를 측정하여 다음 표 5에 나타내었다. Blood samples were collected from the humeral vein of healthy men (15% citric acid-citrate-dextrose for anticoagulant) and centrifuged for 15 minutes at 120 g for 10 minutes at 500 g of platelet-rich plasma. Suspended at 200 million platelets / ml to obtain Washed platelet. As described in Korean Ginseng Journal 21, 132 (1997), the effect of the processed ginseng extract on platelet aggregation induced by collagen was measured and shown in Table 5 below.
상기 표 5에 나타낸 바와 같이, 50% 저해농도를 비교하면 본 발명에 따른 가공인삼 추출물이 기존인삼 추출물보다 52배, 42배 강한 혈소판 응집반응 억제효과를 나타내므로 고혈압, 동맥경화, 뇌졸증 등의 혈액순환관련 질환의 예방과 치료효과가 기대된다.As shown in Table 5, when compared to the 50% inhibitory concentration, the processed ginseng extract according to the present invention exhibited a 52-fold, 42-fold stronger platelet aggregation inhibitory effect than the conventional ginseng extract, such as hypertension, arteriosclerosis, and stroke. The prevention and treatment effect of circulation related disease is expected.
실험예 7: 항암제의 부작용 경감 효과Experimental Example 7: Side effect reduction effect of anticancer agent
항암제 씨스플라틴 투여로 발생하는 부작용 중에서 신장기능 저해효과에 대한 본 발명에 따른 가공인삼의 사포닌 분획 효과를 측정하였다. 체중 200 g 전후의 스프라그도우리계 흰쥐를 각군 6마리씩 5개군으로 하고 대조군(식염수 투여)을 제외한 모든 군에 씨스플라틴 5 ㎎/㎏/5 ㎖ 용액을 복강 투여하였다. 상기 실시예 22에서 얻은 사포닌 분획 추출물 300 ㎎/㎏ , 비교예 4에서 얻은 사포닌 분획 추출물 300 ㎎/㎏을 각각 씨스플라틴 투여 72, 48, 24, 12시간 전에 복강투여하고(사전 투여군), 씨스플리틴 투여 후 12, 24, 48, 72시간에 복강 투여하였다(사후 투여군). 4일째에 실험동물에서 혈액을 채취하고 혈청을 제조하였다. The effect of saponin fraction of the processed ginseng according to the present invention on the renal function inhibitory effect was measured among the side effects caused by the administration of the anticancer drug cisplatin. Sprague-Dawley rats weighing about 200 g were divided into five groups of six in each group, and all groups except the control group (saline administration) were intraperitoneally administered with 5 mg / kg / 5 ml solution of cisplatin. 300 mg / kg of the saponin fraction extract obtained in Example 22 and 300 mg / kg of the saponin fraction extract obtained in Comparative Example 4, respectively, were intraperitoneally administered 72, 48, 24, and 12 hours prior to administration of cisplatin (pre-administration group). It was intraperitoneally administered 12, 24, 48, 72 hours after the administration of the plytin (post-administration group). On day 4, blood was collected from experimental animals and serum was prepared.
BUN(Blood urea nitrogen) 측정용 키트를 사용하여 신장 독성 지표인 BUN을 측정하였다. 우레아제 효소 원액 0.1 ㎖를 완충액 20 ㎖에 혼합한 것 0.02 ㎖에 실험동물에서 채취한 혈액으로부터 제조한 혈청과 우레아 기준액 0.02 ㎖를 첨가하고, 37 ℃에서 15분간 보온한 후 발색시약 2 ㎖씩을 각각 첨가하고 37 ℃에서 5분간 보온한 후 570 nm에서 흡광도를 측정하였다.The BUN (Blood urea nitrogen) measurement kit was used to measure BUN, an indicator of kidney toxicity. 0.1 ml of the urease enzyme stock solution was mixed with 20 ml of buffer solution. To 0.02 ml, serum and urea reference solution prepared from blood from experimental animals were added, and the resultant was kept at 37 ° C for 15 minutes, followed by 2 ml of color reagent. After the addition and warming for 5 minutes at 37 ℃ absorbance was measured at 570 nm.
크레아티닌 측정 키트를 사용하여 신장 독성 지표인 크레아티닌을 측정하였다.Creatinine, a measure of renal toxicity, was measured using the creatinine measurement kit.
실험 동물에서 제조한 혈청 0.5 ㎖에 4 ㎖ 텅스텐 용액을 가하고 강하게 진탕하고 10분 후에 1500 g에서 10분간 원심분리하였다. 원심 분리한 상등액, 크레아티닌 표준약 3 ㎖(대조군은 증류수 3 ㎖), 피크레이트액 1 ㎖, 1.4 M NaOH 0.5 ㎖를 가하여 잘 혼합하고 15분 후에 515 nm에서 흡광도를 측정하였다.4 ml tungsten solution was added to 0.5 ml of serum prepared in the experimental animals, vigorously shaken, and centrifuged at 1500 g for 10 minutes after 10 minutes. Centrifuged supernatant, 3 ml of creatinine standard (control was 3 ml of distilled water), 1 ml of picrate solution and 0.5 ml of 1.4 M NaOH were added and mixed well. After 15 minutes, absorbance was measured at 515 nm.
상기 표 6에 나타낸 바와 같이, 씨스플라틴 투여 후에 실시예 22의 사포닌 분획 추출물을 투여한 사후 투여군에서는 BUN, 크레아티닌치가 정상치에 가깝게 회복함을 알 수 있었다.As shown in Table 6, it was found that in the post-administration group administered with the saponin fraction extract of Example 22 after administration of cisplatin, BUN and creatinine levels were restored to near normal values.
실험예 8 : 뇌 신경세포 보호효과의 측정Experimental Example 8 Measurement of Brain Neuronal Protective Effects
뇌졸증의 치료 및 예방에 대한 효과를 알아보기 위하여 뇌 세포가 글루타메이트 과노출로 사멸하는 것에 대한 가공인삼 사포닌 분획 추출물의 효과를 문헌[J. Neurosci. Res. 53, 426(1998)]에 준하여 측정하여, 다음 표 7에 나타내었다.To determine the effect of treatment and prevention of stroke, the effect of the extract of the processed ginseng saponin fraction on the death of brain cells by glutamate overexposure is described in J. Chem. Neurosci. Res. 53, 426 (1998)].
상기 표 7에 나타낸 결과에서와 같이, 상기 실시예 20에서 얻은 사포닌 분획 추출물은 비교예 4에서 얻은 사포닌 분획 추출물보다 우수한 글루타메이트 신경독에 대한 방어효과를 나타내었다.As shown in the results of Table 7, the saponin fraction extract obtained in Example 20 showed a better protective effect against glutamate neurotoxin than the saponin fraction extract obtained in Comparative Example 4.
실험예 9: 항암작용Experimental Example 9: Anticancer Activity
송아지 혈청 배양액에서 인간 암세포주를 접종하고 48시간 배양한 후 96-웰 배양용기에 1일 동안 계대배양하였다. 그런 다음 가공인삼 추출물을 처리하고 48시간 후 MTT용액을 가하고 불용성 포르마진을 생성시켰다. 원심분리 후 침전층에 디메틸설폭사이드를 가해 포르마진 침전을 용해시켜 570 nm에서 흡광도를 자동평판판독기로 측정하여 50% 세포가 생존하는 농도를 산출하여 그 결과를 다음 표 8에 나타내었다.Human cancer cell lines were inoculated in calf serum cultures and cultured for 48 hours and then passaged in 96-well culture vessels for 1 day. Then, the treated ginseng extract was treated, and after 48 hours, MTT solution was added to generate insoluble formazine. After centrifugation, dimethyl sulfoxide was added to the precipitate layer to dissolve the formazin precipitate, and the absorbance at 570 nm was measured using an automatic plate reader to calculate the concentration at which 50% cells survived. The results are shown in Table 8 below.
상기 표 8에 기재된 바와 같이, 가공인삼 추출물의 사포닌 분획 추출물은 백삼 추출물의 사포닌 분획 추출물에 비해 강력한 암세포성장 억제효과를 나타내었다.As shown in Table 8, the saponin fraction extract of the processed ginseng extract showed a strong cancer cell growth inhibitory effect compared to the saponin fraction extract of white ginseng extract.
실험예 10: 뇌 기능개선 효과의 비교Experimental Example 10 Comparison of Brain Function Improvement Effects
기억력 장애 증상을 갖는 61세- 74세 노인 20명(남자 10명, 여자 10명)을 남녀 반씩 나누어 2개군으로 하고 실시예 2, 참고실시예 2에서 제조한 정제를 1일 2회 1회 2정을 3개월간 복용시키고 문진에 의하여 기억력 장애 증상의 개선 여부를 관찰하여 그 결과를 다음 표 9에 나타내었다.The tablets prepared in Example 2 and Reference Example 2 were divided into two groups of 20 males (10 males and 10 females) having a memory impairment symptom and divided into two groups of men and women. Taking the tablets for 3 months and observing whether the memory impairment symptoms were improved by questionnaire, the results are shown in Table 9 below.
다음 표 9에서 보는 바와 같이 개선되었다와 현저히 개선되었다를 합한 것을 유효한 것으로 판단할 때 본 발명의 가공인삼추출물 실시예 2는 유효율이 80%로써 기존 인삼추출물의 유효율 30%에 비교하면 본 발명의 인삼추출물이 현저하게 기억력 장애 개선을 통한 뇌 기능 개선 효과가 우수함을 확인하였다.As shown in Table 9, the ginseng extract of Example 2 of the present invention has an effective rate of 80%, and the ginseng of the present invention is compared with an effective rate of 30% of the conventional ginseng extract, when it is determined that the combined improvement and remarkable improvement are effective. The extract was remarkably excellent in improving brain function through memory impairment.
실험예 11: 독성시험Experimental Example 11: Toxicity Test
본 발명에 따른 가공인삼 추출물에 대하여 독성실험을 다음과 같이 수행하였다. 구체적으로 상기 실시예 1, 실시예 2의 가공인삼 추출물을 0.2% 트윈 80 수용액에 녹이고 이를 체중 20 g의 ICR계 마우스(군당 20마리)에 각각 5 g/㎏을 경구투여한 다음 7일간 관찰하였으나 사망하는 쥐는 없었다.Toxicity test was performed on the processed ginseng extract according to the present invention as follows. Specifically, the processed ginseng extracts of Examples 1 and 2 were dissolved in 0.2% Tween 80 aqueous solution, and 5 g / kg orally was administered to 20 g of ICR mice (20 mice per group), and then observed for 7 days. No rats died.
이상에서 설명한 바와 같이, 본 발명은 인삼에 오미자, 산사자, 산수유, 모과, 매실, 유자, 탱자, 사과, 석류 및 오렌지 중에서 선택된 1종 또는 2종 이상과 함께 물에서 가열 가공하는 것이 인삼 단독이나 오미자 등 생약재를 각각 추출하여 합하여 사용하는 것보다 증강된 효능을 나타낸다. 이것은 백삼에는 없고 홍삼에만 미량 함유된 진세노사이드 Rg3, Rg5, Rk1 등이 본 발명의 가공인삼 추출물 또는 이의 사포닌 분획 추출물에는 고 농도로 함유되어 있는 것에 기인한 것으로 사료되며 진세노사이드(Rg3 + Rg5)/(Rb1 + Rb2 + Rc + Rd)의 비율이 10 ∼ 45인 것이 특징이다.As described above, the present invention is the ginseng alone or Schisandra chinensis ginseng, hawthorn, hawthorn, cornus, quince, plum, yuzu, tanza, apple, pomegranate and one or more selected from two or more selected from the orange It shows enhanced efficacy than extracting and using each herbal medicine. This may be due to the fact that ginsenosides Rg3, Rg5, Rk1 and the like contained in trace amounts in red ginseng are present in high concentrations in the processed ginseng extract or saponin fraction extract of the present invention. The ratio of) / (Rb1 + Rb2 + Rc + Rd) is 10 to 45.
이러한 가공인삼 추출물은 발기부전 개선, 혈액 순환 개선, 피로회복, 고혈압, 동맥 경화, 항 혈전, 뇌졸중 및 뇌 기능 개선, 항암 보조제 등의 목적으로 의약품 및 건강식품으로 이용될 수 있다. 본 발명에 따른 가공인삼 추출물 또는 이의 사포닌 분획 추출물은 액제, 정제, 과립제, 환제, 캅셀제, 연질캅셀제, 주사제 등으로 제조되어 누구나 편리하게 복용할 수 있다.The processed ginseng extract can be used as medicines and health foods for the purpose of improving erectile dysfunction, improving blood circulation, fatigue, hypertension, atherosclerosis, antithrombosis, stroke and brain function, anticancer supplements. The processed ginseng extract or saponin fraction extract thereof according to the present invention is prepared as a liquid, tablets, granules, pills, capsules, soft capsules, injections, etc., which can be conveniently taken by anyone.
도 1은 본 발명에 따른 가공인삼, 기존인삼의 사포닌 분획 추출물의 박층 크로마토그램을 나타낸 것이다[PG: 비교예 4, GC: 실시예 21, GS: 실시예 22, RG: 비교예 5, RGS: 실시예 28].Figure 1 shows a thin layer chromatogram of saponin fraction extract of processed ginseng, conventional ginseng according to the present invention [PG: Comparative Example 4, GC: Example 21, GS: Example 22, RG: Comparative Example 5, RGS: Example 28.
도 2는 본 발명에 따른 가공인삼, 기존인삼의 사포닌 분획 추출물의 박층 크로마토그램을 나타낸 것이다[1: 비교예 4, 2: 실시예 22, 3: 실시예 21, 4: 실시예 23, 5 : 실시예 24, 6: 실시예 25, 7: 실시예 26, 8: 실시예 27, 9: 비교예 5, 10: 실시예 28].Figure 2 shows a thin layer chromatogram of saponin fraction extract of processed ginseng, conventional ginseng according to the present invention [1: Comparative Example 4, 2: Example 22, 3: Example 21, 4: Example 23, 5: Example 24, 6: Example 25, 7: Example 26, 8: Example 27, 9: Comparative Example 5, 10: Example 28].
도 3은 백삼과 오미자 또는 산사자로 가공된 인삼의 사포닌 분획 추출물을 고속액체크로마토그래피로 분석한 것이다[PG-Q: 비교예 4, GC-1: 실시예 21, GS-1: 실시예 22]. Figure 3 is analyzed by high-performance liquid chromatography of saponin fraction extract of ginseng processed with white ginseng and Schisandra chinensis or mountain lion [PG-Q: Comparative Example 4, GC-1: Example 21, GS-1: Example 22] .
도 4는 본 발명에 따른 가공인삼, 기존인삼의 사포닌 분획 추출물 및 진세노사이드 Rg3의 혈관이완작용을 비교한 그래프이다.Figure 4 is a graph comparing the vascular relaxation effect of the processed ginseng, saponin fraction extract of conventional ginseng and ginsenoside Rg3 according to the present invention.
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| WO2006109979A1 (en) * | 2005-04-12 | 2006-10-19 | Biomedgenomics Co., Ltd. | Novel method for preparing processed ginseng to obtain increased amount of ginsenoside rg5 |
| KR100910585B1 (en) * | 2008-09-10 | 2009-08-03 | (주)보문피앤에프 | Fermented black ginseng and the method of preparing it |
| CN112245443A (en) * | 2020-08-25 | 2021-01-22 | 富力 | Composition of ginsenoside Rg3 and Rg5 and its anti-tumor medicine |
| KR102505329B1 (en) * | 2022-10-17 | 2023-03-07 | 대동고려삼 주식회사 | Method for producing processed red ginseng food using material having improvement effect of cognitive function |
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