JPS63501313A - Cleaning device for many pores - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention] The present invention relates to the cleaning of multiple recesses, for example several rows of recesses in a microtitration plate.

Conventional technology In diagnostic and epidemiological investigations, a large number of biological chemicals can be analyzed in the wells of a microtitration plate. It is common practice to test reactions in bulk. Various stages of such testing On the floor, it is necessary to wash out the depressions. For example, enzyme immunoassay (E , 1. In A, ) (enzyme in + a + uno assay), It is necessary to wash each cavity between each step to remove unabsorbed or unreacted material. It is essential.

A manually operated unit that cleans the wells of a microtitration plate one row at a time as part of the EIA technique. Row washers are currently available. The cleaning process involves vacuum evacuation (e.g. vacuate) L, refill the cavity with a constant volume of rinsing fluid and during immersion By giving a period of time, cleaning the cavity again and repeating the process three or more times. and then carefully rinse each well of the microtitration plate. the hollow is filled again Each time the remaining liberated contents are diluted to approximately 100:1, thus 3 In one cycle, the released contents are diluted 10' to 1. attached structure It is important to carefully control the cleaning to avoid stripping any of the material from the recesses. It is essential. Also, there should be no overfilling that could lead to cross-contamination between the recesses. stomach.

An example of such a manually operated single row washer is the Flow Laboratories Titertek Handivash manufactured by Limited .

One problem that arises with known cleaning equipment is that the flow is not yet evacuated. The body tends to gather in the bottom corners of the depression.

Summary of the invention According to the present invention, in a first aspect, the fluid is vacuum evacuated from the recess of the microtitration plate. A method is provided for , apply suction to the nozzle, and apply suction so that the suction nozzle crosses the bottom of the recess. It includes the step of not moving the microtitration plate horizontally with respect to the pulling nozzle. Suction no By running the slurry across the bottom of the cavity, fluid in the corners of the cavity is effectively removed. be done.

Another problem with the known cleaning device is that the recess is one recess outside the suction nozzle. There can be cross-contamination from fluids carried across from one cavity to the next. That's what I mean.

According to the invention, in a second aspect, a device for removing fluid from a reservoir is provided. The apparatus is provided with a support around the sump and for removing fluid from the sump on the support. a suction nozzle connected to the suction nozzle, and a suction means connected to the suction nozzle; means for lowering the fluid into the reservoir and a suction nozzle lowering the fluid into the reservoir; to apply suction to the suction nozzle before the fluid in the reservoir reaches the suction nozzle. Make sure that the suction nozzle does not penetrate the surface of the fluid in the reservoir when it is pulled up into the tank. and control means for lowering the suction nozzle slowly enough.

Applying suction to the suction nozzle before it is lowered to the fluid in the reservoir This avoids acclimatization of the outside of the suction nozzle with the fluid in the reservoir and prevents this mutual contamination. You can also avoid staining. In one application, the reservoir is a well in a microtitration plate.

In a preferred form, the device includes a fill nozzle and a rinsing fluid for supplying the fill nozzle. a suction nozzle and a suction nozzle, and a suction nozzle and a suction A means for moving the nozzle and a suction tube just above the level of the upper surface of the microtitration plate. Position the pull nozzle and supply while applying suction to the suction nozzle in the lowered position. activating the means to supply rinsing fluid through the filling nozzle to the recess of the microtitration plate; control means adapted to cause the rinsing fluid to overflow from the recess. It is characterized by filling the recess with rinsing fluid to the brim without any problem.

The cleaning cycle consists of precisely controlling the amount of rinsing fluid dispensed into the cavity. It is desirable to control. This control also means that the rinsing fluid is supplied at a constant rate. This can be achieved by controlling the time that the flow control valve is open.

Unfortunately, the output of the pump is usually pulsating, so the flow through the flow control valve is variable. Ru.

According to the present invention, in a third aspect, the pump and the A length of tubing made of flexible, resilient material and a pump output through the tubing. a throttle valve connected to the downstream end of the tube for receiving the force; A liquid supply characterized in that the wall is elastic so as to damp pulsations in the output of the pump. A feeding device is provided.

Brief description of the drawing Particular embodiments of the invention will now be described by way of example with reference to the accompanying drawings, in which: FIG.

FIG. 1 is a top view of a titration plate cleaning apparatus incorporating the present invention.

FIG. 2 is a sectional view taken along the line ■-■ in FIG. 1.

FIG. 3 is a bottom cutout view from below of the device of FIG. 1;

FIG. 4 is a diagram of the spiral cam plate.

Figure 5 is a side view taken along the line ■-■ in Figure 1, with some parts omitted for clarity. Ru.

Figure 6 is a plan view of the device shown in Figure 1, with the cover removed and some parts omitted for clarity. This is an abbreviation.

FIG. 7 is a schematic diagram of the feed pump.

Figures '18 to '3 are cross-sectional views showing various embodiments of the nozzle supply arrangement.

Figure 14 is a partial view passing through the head and the recess, showing the carrier in the recess's vacuum evacuation (air). This shows the two-way movement of the ji.

Figure 15 is a partial view of the head and the recess, showing the size for cleaning the recess to the edge. Shows the kuru.

Description of preferred embodiments Referring to the accompanying drawings, the apparatus for microtitration plate cleaning is shown below the liquid dispensing head 46. A moving carriage 16 is provided. Rinse fluid supply nozzle 48 and suction nozzle 5 A zero run projects downward from the head 46. Pivot 55 (see Figure 5) By moving the head 46 as a center, the nozzles are aligned in a row of depressions in the plate 14. It can be lowered into the sky. accommodating the plate 14 for cleaning operations; Move the carriage 16 under the nozzle to align the - row of recesses under the nozzle. . The cleaning process involves filling the recess with rinsing fluid from the supply nozzle 48 and filling the cavity with rinsing fluid from the suction nozzle 5. 0 to evacuate this fluid. Raising and lowering the dispensing head and carriage movement is achieved by a stepper motor controlled by a pre-programmed microprocessor. This is done by data 36.57. The microprocessor has several different washes cycles and users can select from these cleaning cycles the one that suits their requirements. You can choose.

Referring to FIGS. 1, 2 and 'M3, the housing 9 includes a front wall 5, a rear wall 6, and and a base portion 1o connected to the side walls 7.8, thus providing a rigid support structure. A recess cleaning device is then constructed on this support structure. The cover 11 is attached to the front wall 5 and the rear rests on wall 6 and side wall 8 and extends over the left half of the device as viewed in FIG. .

The microtitration plate 14, shown cut away for clarity, is in place on the carriage 16. Retained. The microtitration plate 14 for use with this device is a bright transparent molded It is an ordinary 8x12 row type made of plastic material. Reaction inside each cavity The degree of reaction inside the hollow is measured optically according to the amount of light that passes through the material. Like a vine, each depression usually has a flat bottom.

The carriage 16 is rectangular with raised lips around its edges for positioning the board. 15, which extends downward toward the central drain hole 13 to collect liquid spilled from the plate. It has an upper surface 17 shaped as a shallow funnel sloping towards the side. The carriage 16 is Moves back and forth in a straight line on one side of the Uzing. The track consists of rail 18 and housing and a groove 19 fixed to the ring. The rail 18 is a side wall of the housing. is attached to the -C cover Il, but the underside of the carriage 16 is attached to one side. (See FIG. 2). The other side of carriage 16 is It is fixed to the upper part 25 of the carriage arm 24. The upper part 25 of the arm 24 is enlarged. and engages in the groove 19 of the cover to support the carriage on its right side and guide it along the track. guide the carriage so that it moves in a straight line. Spring 27 attached to arm 24 presses the underside of the cover 11 to hold the carriage 16 on the cover and expand the arm. Large portion 25 is maintained within groove 19. Due to the opening between the groove 19 and the rail 18, the cover 11 is a droplet tray shaped as a shallow funnel and extending over the length of travel of the carriage 16. A26 is formed. Tray 26 collects spilled fluid from the carriage and then via a pump (not shown) into a central drain hole 20 connected to a suitable tank. Ga A drain filter 21 is disposed inside the drain hole 20.

The carriage 16 is moved along its linear trajectory by a stepper motor 36 to drive a lead screw. It is moved via mechanism 28. The narrow lower portion of the carriage arm 24 through the slot 29 in the cover 11 next to the drop tray 26 from the enlarged upper part. Extends downward and is fixed to the lead screw sleeve 2 3 of the lead screw mechanism 28 .

The lead screw 30 has one end attached to a ball race shaft attached to the rear wall 6 of the housing 9. It is rotatably mounted in the receiver 32 and the other end is adjacent to the front wall 5 of the housing 9. It is attached to the shaft of an attached stepper motor 36. lead screw sleeve 23 is inside the sleeve 23 to provide smoother moving characteristics than with metal equivalents. Through a pair of spaced apart threaded plastic bushings 38 fixed to the side. Engages with the threads of the lead screw. To maintain the threads of the lead screw 30 cleanly, The female lead screw 30 has a threaded cover 40 at one end and a sleeve 23 at the other end. a second base with the other end attached to the sleeve 35 of the stepper motor 36; It is surrounded by a rose-like covering 44. The sleeve is rotated by the rotation of the stepper motor shaft. 23 along the lead screw 30, each shroud 40, 44 expands and contracts to Adapts to changes in the position of the rib 23.

The carriage 16 is moved to a "body rest position FjR" at the front of the cover 11 by a stepper motor 36. (home 5tation)” to the rear operating position below the distribution head 46. can be moved. The rinsing fluid supply nozzle 48 and the suction nozzle 50 are a pair. projecting downwardly from the dispensing head. The spacing between the pairs of nozzles corresponds to the rows of microtitration plates. Corresponds to the interval between the indentations made. In this embodiment, the dispensing head 46 and carriage 16 was used on the standard microtitration plate described above with an 8 x 10 array. It is configured to Thus, 12 pairs of cleaning liquid supply nozzles 48 and suction nozzles 50 is provided. More or fewer nozzle pairs can be installed in a row. It is clear that this is not the case.

The distribution head is compatible, e.g. can be replaced by a head with 8 pairs of nozzles Can be done. Provide two or more rows of nozzle pairs to increase the speed of the cleaning process It is also possible.

Each suction nozzle 50 exits the dispensing head when the dispensing rad 46 is in the vacuum evacuation position. It protrudes vertically downward and can suck fluid from the bottom of the recess of the micro titration plate 14. It is long enough. Each supply nozzle 48 is slightly smaller than its corresponding suction nozzle 50. It is attached at a slight angle and ends in front of the suction nozzle 50. of cleaning solution To enhance the cleaning action, when the cleaning liquid enters the recess from the supply nozzle 48, The supply nozzle 48 is angled relative to the suction nozzle 50 to create a swirling motion. It is best to install the

8-11 illustrate two configurations of supply nozzle 48 that may be used in dispensing head 46. and the dispensing head 46 is lowered into the fill position relative to the cavity to be cleaned. . In a modified example, as shown in FIGS. 12 and 13, the supply nozzle and the suction nozzle are The supply nozzles 48 can be arranged parallel to each other.

The dispensing head 46 is removably connected to an interconnect 52 shown in FIG. A suction hole 54 and a supply hole 56 of the door 46 connect to a suction pump (see FIG. (not shown) and supply pump 80, respectively. Suction hole and supply The holes are connected to suction nozzle 50 and supply nozzle 48, respectively, within the dispensing head. phase The cross section of the supply hole 5G connecting the interconnection part 524 and the supply nozzle 48 is parallel to the side of the nozzle. Having a sufficiently large diameter relative to the cross section that there is no significant pressure between the fluids in different nozzles. There is no difference in strength.

The interconnection 52 is a post 49 fixed to the rear wall 6 of the housing 9 and is connected by a plate 55. This pin is pivotably supported by a second stepper motor 57 (see FIG. 5). It is raised and lowered mainly by bots. The spiral cam plate 60 shown in FIG. It is attached to the shaft 58 of the motor 57. The direction away from the second stepper motor 57 On the surface of the cam plate 60 facing There is a groove 62. A lowering stop arm 66 fixed to the base of the interconnect 52 A pin 64 protruding from the cam plate 60 engages with a groove 62 of the cam plate 60. Second stepper motor 5 7, when the path of the groove 62 passes through the fixing pin 64, the fixing pin 6 4 moves up and down. This causes the lowering stop arm 66 to rise, thus Tilting the interconnect 52 and dispensing head 46 about the pivot leaf spring 55. Itaba The advantage of the thread 55 is that backlash occurs when the direction of movement of the dispensing head 46 is changed. There is no such thing.

Pin 64 is maintained against one wall of groove 62 during movement of dispensing head 46 The interconnect/dispensing head assembly 68 is secured to the rear wall 6 of the housing 9 to a bracket 71 and the interconnect/dispensing head assembly 68. It is urged downward by a spring 70. Inner end of spiral groove 62 of cam plate 60 is tapering in its width, thus lowering the stop arm 66 and therefore distributing it. The head 46 is lowered to the vacuum evacuation position and the lower surface of the lowering stop arm 66 is adjustable. When it comes into contact with the upper surface 73 of the lever 72, the additional force exerted by its own weight and the spring 70 The pin 64 is released from the wall of the groove 62 against which the pin 640 is abutting with a sufficient restraining force. Reba -72 can pivot about point 76 so that When the graduated dial 98 is rotated, the lever 72 is centered at the pivot point 76. pulled upward or downward. This prevents the pin 64 from coming into contact with the sidewalls of the groove 62. dispense head without having to separate and reprogram the movement of the second stepper motor 57. The lowered vacuum evacuation position of door 46 can be adjusted. Lift the dispensing head 46 In order to move the pin 6, the stepper motor 57 rotates the cam plate 60. 4 engages the wall of the spiral groove 62, and the spiral groove 62 6 from the position of contact with the arm of the lever 72. This allows Rad4 to be distributed. 6 to reach the height above the predetermined micro titration plate 17 is the time required for the suction nozzle 50 is independent of the vacuum evacuation position adjustment.

This greatly simplifies programming of the control of stepper motor 57.

Referring also to FIG. 6, the suction hole 54 is connected to a solenoid that controls the use of the suction pump. It is connected to a suction pump via an actuated suction valve 78. In this embodiment, the suction The suction pump is a separate pump located outside the device, but in other embodiments, the The pull pump is arranged within the space 79 inside the housing.

The feed pump 80 shown schematically in FIG. 1. When the piston 81 moves from right to left, the inside of the piston crown The first check valve 85 closes, the fluid is sucked into the space in which the piston 81 has moved, and the second check valve 85 closes. It is pulled to the right of the piston 81 via the stop valve 87. Once the piston 81 moves from right to left If the space to the right of the piston 81 is filled with fluid after moving to the end of the cylinder 83, , the piston returns to its original position and fluid flows through the first check valve 85 to the piston 81. can flow into the space to the left of. Repeated movement of the piston from right to left As a result, the fluid in the space to the left of the piston 81 passes through the third check valve 87 in the cylinder 83. The space to the right of the piston is repeatedly filled. It covers the cylinder 83. The surrounding solenoid is energized to move the piston 81 back and forth.

The delivery response of the pump 80 at startup is instantaneous. The supply is virtually constant over time. This allows the amount of fluid delivered to supply nozzle 48 to be can be calculated based on the length of time the pump is in operation. Of course, this A constant supply of is compromised by the pulsatility of the output characteristics of pump 48. this To overcome this problem, a length of silicone rubber tubing 82 is provided on the output side of the feed pump 80. and the other end of the chicove 82 is connected to an adjustable throttle valve 91. Siri The con rubber tube 82 absorbs energy from the pulse of cleaning fluid and thus Optimal smoothness is achieved by matching 91 to the length of tube 82 .

Smooth cleaning fluid delivery is provided by a solenoid-operated filling control via hard-walled tubing 92. It is connected to the control valve 84. The hard-walled tube is The pressure that immediately causes a flow surge is constricted. It is used to prevent being trapped between the valve 91 and the solenoid valve 84.

The cleaning operation may include as many cleaning and rinsing cycles as necessary for each row. can. These include the first stepper motor 36, the second stepper motor 57, and the A microprocessor (not shown) issues appropriate commands to the lenoid valves 78,84. programmed into software.

When the device is first turned on, the microprocessor controls the stepper motor 36 and 57 (each connected with the corresponding microswitch) It is programmed to perform the

When the carriage 16 reaches the end of its travel towards the rear wall 6, it is attached to the rear wall 6. When the lever 92 comes into contact with a stopped stop (not shown), the sleeve 23 A microswitch 90 attached to the switch is activated. The movement of the dispensing head 46 A similar microswitch (not shown) is closed when the head 46 is fully raised. ).

This program uses a closed microphone that corresponds to a fully raised distribution head. Check for existence of loss switch. If this closure is not detected, the distribution The head has a maximum value of 61 steps or 61 increments of the second stepper motor. rises to.

Once again, the microswitch is asked about the closed state and if the closed state occurs. If it is, the error condition will be indicated on the display panel and the entire cleaning process will be blocked. . However, if closure is detected, the dispensing head will switch to the second step motor. When the microswitch is moved 20 steps downwards, the microswitch questions about an open circuit. be done. Once this is detected, the dispensing head is raised sufficiently. be lifted again within 10 steps of the position.

The microprocessor then performs a similar check routine for carriage movement. Now. If the microswitch corresponding to carriage movement is not closed. If so, the carriage moves in the opposite direction to the maximum value of 1549 steps of the first step motor. On the other hand, the microprocessor uses a microprocessor to detect the closure of the microswitch. Explore the cross switch. Once closure is detected, the carriage moves to the first step The machine is moved forward 20 steps on the machine and the machine is moved forward 20 steps to determine the number of rows and wash cycles to be performed. ready to be programmed according to the nature of the

The number of cleaning routines is determined by the number of steps on the display panel 86 on the housing 90 cover 88. The program is sent to the microprocessor controller by the switch. Appropriate By selecting the button you can select the type of wash cycle and start the cycle. You can select the number of repetitions and select the cleaning fluid used for each cavity. The soak time between cleaning and vacuum evacuation can also be programmed.

The microprocessor provides the following modes of cleaning program:

a) A normal cycle suitable for most applications. In this cycle, the microprocessor The rinser alternately fills the recesses in each row with rinsing fluid to allow immersion. All After all rows are filled, lower the suction nozzle to the bottom of each row of wells and evacuate the wells. put out This cycle is repeated many times.

b) Moving carriage cycle. This cycle is performed during vacuum evacuation of the recess. According to the subroutine, one side 100 of the platelet recess is connected to the suction nozzle. and then bring the other side 101 of the platelet recess up to the suction nozzle (No. (See Figure 14). Finally, it should be washed Before moving the nozzle to the next row, center the nozzle in the recess. Move the jariji. These movements are performed by the stepper motor 36 after the required number of steps. Move the microtitration plate towards the standard microtitration well by moving it forward, then back again. a microphone that instructs the stepper motor 36 to move a distance corresponding to the size of the microphone; Processor.

C) Special cleaning cycle that allows efficient cleaning of residues at the top of the dirt Le. The device is designed so that when the suction nozzle is in the raised position (see Figure 3315), the suction nozzle It is configured to be approximately 2 mm above the level of the top of the microtitration plate. tiny The recess plate will overfill while the dispensing head is in this position, reducing the possibility of overflow. Suction is applied to the suction nozzle for removal. Therefore, the recess is cleaned to the top. It will be done.

d) - Single row wash cycle. This cycle ensures that each column is fully scanned before moving on to the next column. It is the same as a normal cycle, except that the cycle is cleaned.

Raise the dispensing head 46, check the verification routine, and enter the wash mode. A more detailed description of the programmed wash cycle is the programmed wash cycle. the carriage until the first row of indentations is aligned below the row of 12 pairs of nozzles. 16 moves the microtitration plate 17 directly below the dispensing head 46 . The distribution head 46 The pair of drains are lowered into a dispensing position above the final level of cleaning fluid and the cleaning The fluid is controlled by a microprocessor that opens a solenoid-operated fill control valve for a predetermined period of time. It is supplied to the supply nozzle 48 based on the command from. Each supply nozzle to the microtitration plate The angle of the hole 48 allows the fluid to swirl around the recess as described above and The identified pump characteristics ensure that the amount of fluid being regulated is accurate and Guaranteed that it will not spill into the water. of the jet of fluid exiting the supply nozzle 48. The force may be insufficient to wash away the reacted antigen/antibody absorbed into the cavity walls. It is located.

After the soak time, the dispensing head moves towards the recess of the microtitration plate to the vacuum evacuation position. be lowered. Once this is done, each suction nozzle 50 has a surface of fluid in its corresponding recess. Before encountering the surface, a solenoid-operated suction control valve 78 is opened to begin suction action. Ru. This sequence divides the fluid level so that it falls at a faster rate than the nozzle. When lowering the descending speed of the distributing head 46, the fluid does not touch the sides of the nozzle. inside the suction nozzle 50 (and thereby without contaminating the sides of the nozzle). be drawn into.

The feature of the flat bottom of the microtitration plate is that the proportion of the cleaning solution is It remains in the corner due to surface tension. Minimize the amount of fluid remaining in the cavity and To significantly improve the dilution of unreacted material in the wells, By moving back and forth with the dispensing head 46 in the empty discharge position, the suction nozzle 50 becomes more The microprocessor is placed in mode (C) so that large amounts of fluid can be drawn up. ) is recommended. Once the suction action has been performed, the dispensing head 46 The microtitration plate 140 is lifted and the washing cycle is carried out in the same recess or in the next row of microtitration plates. repeated in the hollow.

Each supply nozzle 48 is located next to a corresponding suction nozzle 50 and thus The removal of fluid adhering to the fluid is not limited by the presence of the supply nozzle 48 Therefore, it is possible to move the carriage without compromising the integrity of the emptying action. be.

The spacing of each indentation is the same for a given type of microtitration plate, but It is necessary to be able to finely adjust the attitude of the dispensing head 46. This adjustment Loosen the screw 93 that fixes the This is done by adjusting. Determine the depth of movement of the suction nozzle 50 into the recess. In order to be able to change, an adjustment lever is used as described above. this hollow The depth of can vary according to the nature of the microtitration plate used. The bottom of the depression If the surface is not flat, the suction nozzle 50 may be shuffled back and forth. ling) is decreased or programmed from that cycle and accordingly The working depth of the suction nozzle 50 will change accordingly.

Procedural amendment (formality) Mr. Kunio Kogawa, Commissioner of the Patent Office t〆1%, q2nu/ 1. Indication of incident PCT/GB8610 O5522, name of invention Many buds cleaning equipment 3. Person who makes corrections Relationship to the case: Applicant Name: Flow Laboratories Limited 4, Agent 5. Date of amendment order: February 9, 1985 6. Subject of amendment: Translation of drawings international search report

Claims (11)

[Claims] 1. In a method for emptying a fluid from a recess in a microtitration plate, Lower the suction nozzle into the recess of the micro titration plate, apply suction to the suction nozzle, Place the microtitration plate against the suction nozzle so that the suction nozzle crosses the bottom of the well. The method includes each step of flattening. 2. In a device for directly discharging fluid from the recesses of a microtitration plate, The suction nozzle attached to the head, the support for the microtitration plate, and the selection of the microtitration plate. The support is moved horizontally so that the selected depression is positioned directly under the suction nozzle. a head for lowering the nozzle to the bottom of the selected recess; a means for lowering the head, a source of air for applying suction to the nozzle, and a means for lowering the head. While applying suction to the suction nozzle, the suction nozzle control means configured to actuate the support moving means across the bottom; A device characterized by comprising: 3. The head also supports a cleaning fluid supply nozzle, the cleaning fluid supply nozzle being one supply. A supply nozzle and one suction nozzle are lowered into each recess of the microtitration plate. Claim 2, characterized in that the suction nozzle is arranged in a pair with the suction nozzle. The device described. 4. The supply nozzle is connected to the cleaning fluid source via the supply valve, and the suction nozzle connects the suction valve. The control means operates the supply valve and the suction valve in a cycle. The device according to claim 3, characterized in that it is configured to move. . 5. In a device for removing fluid from a reservoir, a support for the reservoir; a suction nozzle for removing fluid from a reservoir on the body; and a suction nozzle connected to the suction nozzle. A suction means, a means for lowering the suction nozzle toward the reservoir, and a nozzle. and fluid for applying suction to the suction nozzle before lowering it to the fluid in the reservoir. When the fluid is pulled up into the suction nozzle, the nozzle does not penetrate the surface of the fluid in the reservoir. control means for lowering the nozzle slowly enough to A device for removing fluid from a reservoir, characterized in that: 6. Claim 5, characterized in that the reservoir is a recess in a microtitration plate. equipment. 7. In a device for cleaning the recesses of a microtitration plate, the filling nozzle and a means for supplying rinsing fluid to the nozzle, a suction nozzle, and a means for supporting the microtitration plate; means for moving the suction nozzle relative to the support for Position the suction nozzle just above the level of the surface and apply suction to the suction nozzle. while supplying rinsing fluid through the filling nozzle to the wells of the microtitration plate. control means adapted to actuate the supply means, whereby the rinsing fluid is supplied. Characterized by filling the cavity with rinsing fluid to its edges without overflowing from the cavity. A device for cleaning the recesses of a microtitration plate. 8. a pump and a length of flexible and resilient material connected to the output side of the pump; tube and the downstream end of the tube to receive the output of the pump through the tube. a throttle valve connected to the tube, the walls of the tube damping the pulsations in the pump's output. A liquid supply device characterized in that it is elastic. 9. 9. Device according to claim 8, characterized in that the throttle valve is adjustable. 10. Claims characterized in that the output side of the throttle valve is connected to a flow control valve. 9. The device according to paragraph 8 or 9. 11. A plurality of supply nozzles, a supply source of cleaning fluid, and claim 2 or 3. a liquid supply device as described in Section 1, and the inlet of the pump is connected to a source of cleaning fluid. and the throttle valve is used to supply the cleaning fluid through the nozzle and into the cavity of the microtitration plate. for cleaning the recesses of a microtitration plate, which is characterized by being connectable to the nozzle of equipment.