JPS6323502B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to an electrophoresis sample dish used in an electrophoresis apparatus.

[Prior Art] As shown in FIG. 4, a sample dish used in an automatic electrophoresis apparatus is one in which a plurality of sample dispensing recesses 1a are formed in one sample dish 1. A different sample 2 is included. A sample supply device that supplies the sample dish 1 while preventing moisture evaporation from the sample 2 is disclosed in, for example, Japanese Patent Application No. 54-57845 filed by the present applicant (Japanese Unexamined Patent Application Publication No. 55-578).
149826)). That is, the sample supply device is as shown in FIG.
The upper opening 3a and the inner dimensions are almost the same as the outer dimensions of the sample dish 1, and the size is such that a plurality of sample dishes 1 can be stacked vertically and accommodated, and the lower end is large enough to allow one sample dish 1 to pass through. A sample plate storage container 3 having a front opening 3b and placed on a support stand 4, a sample plate protection cover 5 placed on the uppermost sample plate 1, and a lowermost sample plate 1. The front opening 3b
It is equipped with a piston-type sample transfer mechanism 6 for transferring the sample plate 1 to the sample attachment position A through the sample transfer mechanism 6, and a receiving tray 7 for storing the used sample plate 1. The sample 2 is pushed out and moved to the sample attachment position A, where the sample 2 is attached to the coating tip 9 of the sample applicator 8, and then the sample 2 attached to the coating tip 9 is applied onto the support 10 at the sample coating position B. During this time, the sample transfer mechanism 6
The second sample pan 1, which is now at the lowest position, is then pushed out to the sample attachment position A. At this time, the first sample tray 1, which had been at the sample attachment position A, is moved to the second sample tray 1. The sample plate 1 is pushed and dropped into the receiving tray 7 by movement, and the above-mentioned operations are repeated in sequence, while the sample plate 1 remains in the sample plate storage container 3, that is, until it is moved to the sample attachment position A. During this time, a sealed state was maintained by the protective cover 5 or the sample plate 1 stacked immediately above, thereby preventing moisture from evaporating from the sample 2.

[Problems to be Solved by the Invention] However, this sample supply device has the disadvantage that the used sample plate is dropped into the receiving tray, making it impossible to reuse the sample for reexamination, etc. had.

The present invention was made in view of the above drawbacks, and an object of the present invention is to provide a sample dish for electrophoresis suitable for supplying a sample.

[Means and effects for solving the problems] The present invention provides a sample plate ejector provided in a sample plate storage container for storing a plurality of sample plates in a stacked manner at the edge of a sample plate having a plurality of recesses for storing samples. By providing an inclined portion in the direction of the opening for pushing up the sample plate cover, the discharged sample plate can be covered with the sample plate cover.

[Embodiment] An embodiment of the present invention will be described with reference to FIGS. 1A and 1B and FIG. 2. Note that the same members as in FIGS. 4 and 5 will be described with the same reference numerals.

In FIGS. 1A and B, 1' has a plurality of sample dispensing recesses 1'a formed on the leading edge side of its top surface, an inclined part 1'b formed on its rear edge, and its bottom surface. The basic structure of the sample plate 3' is that of the conventional sample plate storage container 3, in which a pair of left and right engagement holes 1'c are formed in the rear edge portion of the sample plate feed lever, which will be described later, into which the upper end of the sample plate feed lever can be engaged. The sample plate storage container 4', which is the same but further has a rear opening 3'c at its lower end large enough for one sample plate 1' to pass through, has the same basic structure as the conventional support stand 4. In addition, the support stand 5' has a pair of long left and right slots 4'a on the top wall thereof, through which the upper end of the sample plate feed lever (described later) passes through, and 5' has a basic structure similar to that of the conventional sample plate protection cover 5. This is a sample plate protection cover which is the same but further has a pair of left and right engagement holes 5'a formed in the rear edge side portion of the bottom surface thereof, into which the upper end of a sample plate feed lever, which will be described later, can be engaged. Reference numeral 11 denotes a fixing member that is removably fixed on the support stand 4' near the rear of the sample plate storage container 3', and has a hole 11a in its lower end that is large enough to allow one sample plate 1' to pass through. , 12 is a horizontal support rod fixed to the fixing member 11 so as to extend rearward, and 13 is a support rod 1.
The position of the bottom surface (height H' from the top surface of the support table 4') is the same as that of the sample plate 1' placed on the support table 4. These are a plurality of sample plate covers that are normally lower than the top surface (thickness H of the sample plate 1') and are arranged in series toward the rear. 14 is a support member fixed on the bottom wall of the support base 4'; 15 is a support member 14;
16 is an upper lever fixed to the upper end of the vertical support shaft 15;
7 is a lower lever fixed to the lower end of the vertical support shaft 15; 18 is interposed between the upper lever 16 and the support member 14; A spring 19 is a support member 14 that provides an upward movement habit.
A stopper 20 is fixed to the upper end and restricts the lifting of the vertical support shaft 15, upper lever 16, and lower lever 17 to a predetermined position. The upper end 20b can pass through the slot 4'a of the support base 4' and engage with the engagement hole 1'c of the sample plate 1'. A pair of left and right sample plate feed levers are provided with a longitudinally long slot 20c in the center thereof, and 21 is a disk 2 rotated by a motor (not shown).
This is a drive pin which is fixed at a predetermined position on the outer circumference of the drive pin 2 and whose tip engages with the slot 20c. Then, due to the displacement of the drive pin 21 due to the rotation of the disk 22 in the clockwise direction in FIG. Since the upper lever 16 is pushed down by the stroke h from the lift restriction position against the elastic force of the upper lever 18, the sample feed lever 20 is also lowered by the stroke h from the lift restriction position. 2. At this time, the drive pin 21 comes off the upper lever 16, so the sample feed lever 20 moves up by the stroke h due to the elasticity of the spring 18 and returns to the rise restriction position.) Point position c (position shown by the one-dot chain line in FIG. 2. At this time, the drive pin 21 has not yet pushed down the upper lever 16,
remains in the rise restriction position. ) is made to swing and move up and down in sequence, and this is repeated. Further, the forward movement distance of the sample pan 1' from the front opening 3' due to the displacement of the upper end 20b of the sample feed lever 20 from the reset position a to the front dead center position b is determined by the front-to-back width w of the sample pan 1'. The sample dispensing concave portion 1'a of the sample dish 1' is set to be sufficiently exposed from the sample dish storage container 3'. or,
The backward movement distance from the rear opening 3'c of the sample pan 1' due to the displacement of the upper end 20b of the sample feed lever 20 from the front dead center position b to the rear dead center position c is determined from the front-to-back width W of the sample pan 1'. is also set to be large.

Since the sample supply device is constructed as described above, firstly, by sequentially inserting a large number of sample plates 1' into the sample plate storage container 3' from the upper opening 3'a, the dimensions of the upper opening 3'a and the inside are adjusted. Since the outer dimensions are approximately the same as the sample trays 1', the sample trays 1' always fall horizontally and are accurately stacked in the sample tray storage container 3'. Thereafter, the sample plate protection cover 5' is also inserted through the upper opening 3'a and placed on top of the uppermost sample plate 1'. At this time, the upper end 20 of the sample plate feed lever 20
b is stopped at reset position a. Next, when the disk 22 is rotated, the drive pin 21 is first disengaged from the upper lever 16, so the upper end 20b of the sample plate feed lever 20 rises and engages with the engagement hole 1'c of the lowest sample plate 1'. Then, as the upper end 20b moves to the front dead center position b, the lowermost sample tray 1' passes through the front opening 3'b of the sample tray storage container 3', and the sample dispensing recess 1'a is inserted into the sample tray 1'. It is moved forward until it reaches the attachment position A. Then, as in FIG. 5, the sample 2 in the sample dispensing recess 1'a is attached to the tip 9 of the sample applicator 8, and the sample 2 is further attached to the tip 9 at the sample application position B. Sample 2 is applied onto support 10. In this case, since the forward movement distance of the lowest sample plate 1' from the front opening 3'b is set smaller than the front-to-back width of the sample plate 1',
The rear part of the lowest sample plate 1' remains in the sample plate storage container 3', and therefore the second and higher sample plates 1' from the bottom cannot be lowered. Next, the upper end 20b of the sample plate feed lever 20 moves to the rear dead center position, so that the lowest sample plate 1' to which the sample has been attached is moved to the rear opening 3'c of the sample plate storage container 3' and the fixing member 11. The sample plate cover 13 is moved backward through the hole 11a, and moved while pushing up the sample plate cover 13 by the action of the inclined portion 1'b of the sample plate 1'.
It will stop at the position that matches. And during this time,
The remaining sample pans 1' and protective cover 5' are lowered by one sample pan due to their own weight, and the second sample pan 1' is positioned at the lowest position. Subsequently, the upper end b of the sample plate feed lever 20 begins to move forward again, but at the same time, the drive pin 21 begins to push down the upper lever 16, so the upper end 20b begins to descend, and is lower than the forward movement. Since this is faster, the upper end 20b disengages from the engagement hole 1'c without moving the sample plate 1' forward and stops at the reset position a. In this way, by sequentially repeating the above operations, the sample 2 is supplied to all the sample plates 1' in the sample storage container 3', and all the sample plates 1' are covered with the sample plate cover 13 after the sample is attached. sent down. After the analysis is completed, the fixing member 11 supporting the sample plate cover 13 is moved to the horizontal support rod 1.
The used sample dish 1' is recovered by removing it together with the sample dish 2 from the support stand 4'.

According to the above embodiment, each sample plate 1' in the sample plate storage container 3' is maintained in a sealed state by the protective cover 5' or the sample plate 1' stacked immediately above. Evaporation of moisture from Sample 2 is prevented. Further, even after the sample is attached, each sample plate 1' is sealed by the sample plate cover 13, so that moisture from the sample 2 is prevented from evaporating. In addition, since evaporation of moisture from the used sample 2 is prevented in this state, the sample 2 can be reused for re-inspection or the like. Furthermore, since no member is located between the sample attachment position A and the sample application position B, the distance between both positions A and B is shortened, and as a result, the travel time of the application destination 9 is shortened, and the application destination 9 The sample 2 adhered to the substrate 10 does not need to be dried much before being coated on the support 10.

In the case of the above embodiment, in order to allow the sample plate 1' to fall horizontally into the sample plate storage container 3', the inner dimensions of the upper opening 3'a are approximately the same as the outer dimensions of the sample plate 1'. the same, but instead the third
As shown in the figure, U-shaped recesses 1'd are provided at the left and right ends of the sample dish 1', and vertical guides (not shown) into which the recesses 1'd can fit are provided on the left and right inner walls of the sample dish storage container 3'. It's okay.

[Effects of the Invention] According to the electrophoresis sample dish of the present invention, the sample can be supplied to an electrophoresis apparatus while preventing moisture from evaporating from the sample.

[Brief explanation of the drawing]

FIG. 1A is a perspective view of an embodiment of the sample dish of the present invention, FIG. 1B is a cross-sectional view of FIG. 1A, and FIG. 2 is a vertical cross-section of a sample supply device using the sample dish of the present invention. 3 is a perspective view of another embodiment of the sample dish of the present invention, FIG. 4 is a perspective view of a conventional sample dish, and FIG. 5 is a vertical sectional view of a sample supply device using a conventional sample dish. It is. 1'...Sample dish, 1'a...Dispensing recess, 1'b
...Slope part, 2...Sample.

Claims (1)

[Claims] 1. To push up the sample plate cover in the direction of the sample plate discharge opening provided in the sample plate storage container that stores multiple sample plates stacked on the edge of the sample plate that has multiple recesses for storing samples. A sample dish for electrophoresis, characterized in that it is provided with an inclined part.