JPS63119775A - Blood separation apparatus - Google Patents

Abstract

(57) [Abstract] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a blood separation device that automatically separates blood components in a centrifuged blood bag into separation bags.

[Conventional technology] In recent years, the adverse effects of whole blood transfusion have been viewed as a problem.

BACKGROUND ART Blood component transfusions have become popular with the aim of reducing the physical burden on patients and side effects caused by immunity as much as possible by transfusing only those components of blood that are necessary for the patient.

On the other hand, in order to make a component preparation, it is necessary to fractionate the blood in a blood bag into each component using a centrifuge and separate each component into separate bags.

Therefore, in the past, a separation stand as shown in Figure 8 was used to manually separate the plasma in the centrifuged blood bag, and a tube sealer was used to seal the tube of the separation bag. I make it and seal it.

That is, the blood bag 1 that has undergone centrifugation is set in the hanging bottles 3, 3 of the separation stand 2 so that the blood cells do not fly up. At this time, the tube 5 of the separation bag 4 is
After stopping the middle part with a Kocher 6, the separation needle 7 attached to the tip of the tube 5 is pierced through the discharge port 1a of the blood bag 1, and both bags 1.4 are communicated with each other. Next, by removing the lever 8 of the separation stand 2 from the hook 9, the blood bag 1 and separation stand 2 are rotated via the fixing plate 10 of the separation stand 2 and the lever 8 which is always urged in a predetermined direction by a spring (not shown). While sandwiching it between the pressing plate 11,
The Kochel 6 that closes the middle part of the tube 5 is removed, and the plasma is separated into the separation bag 4. When plasma moves to the separation bag 4 and blood cells begin to enter the tube 5, the tube 5 is closed with the Kocher 6, the press plate 11 is returned to its original position, and the lever 8 is locked to the hook 9.

Next, pressure is applied to the separation bag 4 by hand to open the container 6 to return air to the blood bag 1 side, and the tube 5 is closed with the container 6.

[Problems to be Solved by the Invention] As described above, conventional separation operations have mainly been performed manually, which has the following drawbacks.

(1) It places a heavy burden on workers. That is, it is labor-intensive, and the separation operation is performed for each bag, and one person can only handle two bags at a time. As a result, they may be overwhelmed with work.

(2) Separation stands, scales, tube sealers, and other equipment are required separately, and a large installation space is required.

(3) Experience and intuition are required to control the amount of blood components.

[Means for Solving the Problems] The present invention has been made for the purpose of solving the above-mentioned problems, and includes the following configuration in order to solve the problems.

That is, a blood bag pressurizing means that holds a centrifuged blood bag and pressurizes the blood bag at a predetermined pressure, and a blood bag pressurizing means that holds at least two separation bags that communicate with the blood bag via a tube and that controls the weight of the separation bags. A holding measuring means for measuring the amount of liquid, a regulating means disposed midway through the tube between the blood bag and each separation bag and regulating the amount of liquid movement within the tube, and a holding measuring means that uses the measured weight information of the holding measuring means as part of the control information. and control means for controlling the regulating means.

[Operation] In the above configuration, the control means is pressurized by the blood bag pressurizing means, and by controlling the regulating means, the blood flowing out from the bag is automatically stored in separate bags for each component.

[Embodiments] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the drawings.

1(A) to 1(C) are external views of one embodiment of the present invention, in which FIG. 1(A) is a plan view, FIG. 1(B) is a front view, and FIG. 1(C) is a plan view. is a side view.

In FIG. 1, 21 is a pressure plate that pressurizes the blood bag 51, 22 is a stopper that restricts the movement of the pressure plate, 23 is a measurement plate, and 24 is a weight indicator window, which is placed on the measurement plate 23. The measurement result of the weight of the object will be displayed. This measurement uses a spring weighing mechanism using a parallel link mechanism, which has a built-in rotary encoder and outputs a signal proportional to the measured weight.

25 is an adjustment handle that adjusts the vertical position of the photosensor 29; 26 is a worm gear fixed to the rotating shaft of the adjustment handle 25; 27 is a pinion gear that is always engaged with the worm gear 26;
9 is fixed. 28 holds a blood bag 51;
The pressurizing holding section 29 that pressurizes with the pressurizing plate 21 is a photosensor that detects concentrated red blood cells in the blood bag 51 suspended from a hook pin (not shown) of the pressurizing holding section 28 .

Further, 31 is a hanger for suspending the separation bag, and the lower part thereof is a spring weighing mechanism using a parallel ring mechanism, and a rotary encoder is incorporated therein, and a signal proportional to the measured weight is output. Reference numeral 32 is a hanger for suspending a separation bag that can be attached as an option. 33 to 35 are clamp valves that clamp the tube sealer and control the flow of liquid in the tube, and when the valves are closed, the flow of liquid is stopped. 40 is an operation panel, which includes a power switch 15, a select switch 36 for mode selection,
There is a start switch 37 and a stop switch 38.

Further, 41 and 42 are handles, and 43 is a maintenance panel. Further, 51 to 54 are bags for storing blood components, and are also attached to the hanger 32 as necessary.

The configuration of the electrical control section of this embodiment will be explained with reference to the block diagram of FIG.

In the figure, 100 is a control unit that controls the entire embodiment according to the program stored in the program cassette 200 attached to the connector unit 115 of the program cassette control unit 114, and 101 is a spring containing the pressure plate 21. This is a pressurizing cylinder control unit that controls the pressurizing cylinder 102 that maintains the pressurizing cylinder 102 in a non-pressurized state against the pressure, and the pressurizing cylinder 10
2 is stopped, the pressure plate 21 pressurizes the set bag by spring pressure from a spring (not shown). 10
3 calculates the weight of the bag from the output of a rotary encoder 105 that outputs a signal proportional to the weight of the bag suspended on the hanger 31 of a hanger weight measurement unit 104 disposed below the hanger 31, and outputs it to the control unit 100. A hanger weight conversion unit 106 calculates the weight of the bag based on the output of a rotary encoder 108 that outputs a signal proportional to the weight of a weight measurement unit 107 that measures the weight of the bag placed on the measurement plate 23, and sends it to the control unit 100. This is a weight conversion unit that outputs.

110-112 are clamp valves 1-3 (33-35)
113 is a RAM;
Reference numeral 16 denotes a sound output control section that controls and controls sound output such as a chime sound from the speaker 117. Further, 29 indicates a photosensor shown in FIG. 1, and 36 to 38 indicate switches similarly shown in FIG. 1.

The control of this embodiment with the above configuration is shown in FIGS.
This will be explained below with reference to the flowchart of B).

First, in step S1, select the 1" key of the select switch 36 to set the first sequence. Next, each bag is attached to each predetermined position. Here, the inside of the pressure plate is subjected to strong centrifugation (for example, 4-1 at 5500G
0 minutes), set the blood bag 51, hang the child bag 1 (52) on the hanger 31, and attach the measurement plate 2.
A child bag 3 (53) and a grandchild bag (54) are placed on the blood bag 1.3, and in the following step S3, each bag and the blood bag are communicated with each other by a tube via a clamp valve 1.3 (33, 35). This state is shown in FIG. 1(A), and in this case, the hanger 32, clamp valve 2 (34)
No tubes will be installed.

Here, the blood bag 51 subjected to the strong centrifugation process is separated according to each blood component, and is in the state shown in FIG. 4. That is, there is a concentrated red blood cell layer 61 at the bottom and a white blood cell layer 62 above it.
Furthermore, a platelet layer 63 is formed, and a plasma layer 64 is formed at the top.

In the subsequent step S4, as a result of setting the blood bag 51 at the pressure plate 21 position, the detection position of the photosensor 29 is at a predetermined position (for example, about 32 mm) from the upper surface of the blood bag 51 (position A in FIG. 4). ). If the photo sensor 29 is not at the predetermined position, the photo sensor 29 is moved up and down by rotating the adjustment handle 25 in step S5 to adjust it to the predetermined position, and the process proceeds to step S6. This is because the plasma layer 64 allows light to pass through, so the user can adjust the liquid volume by controlling the blood components to be sent to the child bag 1 (52) until the photocoupler 29 detects the concentrated red blood cell layer 61. This is because it is possible.

Since preparation for blood separation is now complete, the "start" switch 37 is pressed in step S6. Then, in the following step S7, clamp valve 2 (34) and clamp valve 3 (35) are closed to prevent liquid from flowing through the set tube. This prevents liquid from flowing into child bag 2 (not set in this example) and child bag 3 (53). Then, in step S8, the pressure cylinder control unit 101 is instructed to release the pressure cylinder 102, and the pressure plate 21 presses down the blood bag 51 due to the pressure plate spring pressure (
pressurize). Therefore, as shown in step S9, the blood components in the blood bag 51 are removed from the opened clamp valve 1.
(33) to child bag 1 (52). Therefore, in the subsequent step 310, it is checked whether the photosensor 29 has detected the concentrated red blood cell layer 61, and if it has not detected it, the process waits for detection. When the red blood cell level of the blood bag 51 is detected by the photocoupler 29, the process proceeds from step S10 to step Sll, and the valve control unit 1 (t
to) and close clamp valve 1 (33). As a result, the flow of liquid into the child bag 1 (52) is stopped, and only the plasma layer 64 in the blood bag 51 is transferred to the child bag 1 (52).
This means that it was transferred to (52).

Subsequently, in step S12, the empty child bag 3 (53) and grandchild bag (53) placed on the measurement plate 23 are
54) Measure the weight! The i amount information is converted into the weight converter 106
(read from) and store it in RAMll3. Then, in step S13, the valve control unit 3 (112) is controlled to open the clamp valve 3 (35), and as shown in step S14, the blood components in the blood bag 51 are transferred to the child bag 3 (53). . The control unit 100 performs step S15.
The measured weight from the weight converter 106 is monitored, and it is checked whether the "first set amount" (for example, 70 g) specified by the program cassette 200 has flowed in (or whether the weight has increased). Here, when the transfer of the "first set amount" is completed, the process proceeds to step 316, where the valve control section 3 (112) is controlled to close the clamp valve 3 (35), and the child bag 3
Stop the liquid flowing into (53). And step S17
to control the pressurizing cylinder control section 101 and pressurizing cylinder 1.
02 to open the pressure plate 21. As a result, the child bag 3 (53) contains platelets, some white blood cells and red blood cells, and the remaining plasma.

Next, in step 51B, valve control unit 1 (110) is controlled to open clamp valve 1 (33), and step S
As shown at 19, blood components (plasma) from the child bag 1 (52) naturally fall into the blood bag 51, which is the parent bag. Therefore, in the subsequent step S20, the weight of the child bag 1 (52) suspended on the hanger 31 is read from the hanger weight converter 103, and
"Set bag weight" specified by 0 (e.g. 20
g) Monitor whether blood has been injected, and when the set bag weight J is reached, proceed to step 521, control the valve control unit 110 to close the clamp valve 1 (33), and close the blood bag 5.
Stop the flow of liquid into 1.

As a result, a predetermined amount of plasma has flowed into the remaining concentrated red blood cells into the blood bag 51. As a result, the hematocrit value of concentrated red blood cell fluid can be corrected to, for example, 80% or less.

In the next step S22 and step 523, since the blood component separation has been completed, the acoustic output control unit 116 is controlled to emit several chimes from the speaker 117, and the start lamp 118 (start switch 3 which is an illuminated switch) is activated.
7) blinks. The operator thereby recognizes the end of the blood separation process, fixes and closes the tubes connecting each bag with clips, etc., and presses the end switch 38. Since the control unit 100 monitors the input of the end switch 38 in step S24, the process proceeds to step S25 based on the input of the end switch 38, and controls the valve control units 110 to 112 to control each clamp valve 3.
3 to 35 are released and the process ends.

In addition, although the above explanation describes an example in which the child bag 2 is not suspended from the hanger 32, it is also possible to store an additive liquid for blood preservation in the child bag 2 to be supplied to the blood bag 51 as needed.
Following step S17, a predetermined amount of this additive liquid may be supplied to the blood bag 51.

In this way, the blood bag 51 and child bag 1 (52),
Among the blood components separated into child bag 3 (53), concentrated red blood cells 61, white blood cells 62, platelets 63, and some plasma 64 transferred to child bag 3 (53) can be further separated. be.

In this case, this child bag 3 (53) is subjected to mild centrifugation. The mild centrifugation process is performed, for example, at 800G for about 5 minutes. By this process, child bug 3 (53)
is in the state shown in FIG. 5, with liquid platelets 65 at the top. By performing the mild centrifugation process in this manner, liquid platelets 65 are produced. Thereafter, the process according to FIG. 6 may be executed.

First, in step S50, the "2" switch of the select switch 36 is pressed to set the second sequence. Then, in the subsequent step S51, the child bag 3 (53) in the state shown in FIG. A tube communicates between the bags, and the tube is passed through the clamp valve 3 (35). Then, as in step S4, it is checked whether the photosensor 29 is at a predetermined position or not, and if it is not at the predetermined position, the adjustment handle 25 is rotated in step S53 to set the photosensor 29 at a desired position. Then, the process proceeds to step S54, where the start switch 37
Press to input. When the control unit 100 detects that the start switch 37 is turned on, the control unit 100 controls the pressure cylinder control unit 101 to open the pressure cylinder, and moves the pressure plate 21 to the child bag 3 (53) by a pressure plate spring force (not shown). Pressure contact. Subsequently, in step S56, clamp valve 3 (35)
is released, and the child bag 3 (which is pressed by the pressure plate 21)
53) is transferred to the grandchild bag (54). Then, in the following step S58, it is monitored whether the photosensor 29 has detected red blood cells, and when the photosensor 29 detects red blood cells, the process proceeds to step S59, where the valve control section 110 is controlled and the clamp valve 3 (35) is closed. . Then, in steps S60 to S62, a chime sound is output as in steps 322 to 24, the start lamp 118 is blinked, and the end switch 38 is waited for to be pressed manually. Here, when the operator recognizes the end of the second sequence process, performs the necessary processes, and presses the end switch, the process proceeds from step S62 to step S63, where the clamp valve 1 is opened and the process ends.

By controlling in this manner, only the liquid platelets 65 can be separated from the concentrated red blood cells 61, white blood cells 62, and liquid platelets 65 in the child bag 3 (53).

The above explanation has been made regarding an example in which the photosensor 29 is used to control the separation of the liquid content from the child bag 3 (53), but in addition to this, the device of this embodiment also performs this separation by weight measurement. be able to. An example in which this separation is carried out by weight measurement will be explained with reference to the flowchart of FIG.

First, in step 5100, the select switch 36 is set to "3".
Press the switch manually to set the third sequence. Then, in the following step 5101, the child bag 3 (53) in the state shown in FIG. 5 is set on the pressure plate 21, and
Place the grandchild bag (54) on the measurement plate 23. In the next step 5102, the tube is clamped to the clamp valve 3 (3
5) Through child bag 3 (53) and grandchild bag (54)
Clamp them in the middle to communicate. and step 5
At step 103, the start switch 37 is pressed. When the control unit 100 detects the press input of the start switch 37, the process immediately proceeds to step 5104, and the valve control unit 110
control to close the clamp valve 3 (35). Then, in step 5105, the weight of the grandchild bag (54) placed on the measurement plate 23 is measured and stored in the RAM 113. Then, in step 5106, the pressure cylinder control unit 101 is controlled to open the pressure cylinder, and the pressure plate 21 is pressed against the child bag 3 (53) by the pressure of a pressure plate spring (not shown). Subsequently, in step 5107, the clamp valve 3 (35) is opened, and as shown in step 3108, the blood components of the child bag 3 (53) are transferred to the grandchild bag (54) by pressing with the pressure plate 21.

Then, in step 5109, the weight converter 106 reads and monitors the weight of the grandson bag (54), and the amount specified by the program cassette 200 (for example, about 40g) is monitored.
) is sent eight times, and it is checked whether the weight of the grandchild bag (54) has reached a predetermined amount. When the predetermined amount of 8 feeds is completed, the process proceeds to step 5110, and thereafter steps 5110 to 5114 and steps 359 to S63 in FIG.
Executes the same process as .

As explained above, the blood separation device of this embodiment can be used in multi-bag systems (e.g., double bag, triple bag, quadrable bag, SAG bag system, etc.) used for blood component preparations by centrifugation. , is a device that automatically separates each blood component (red blood cells, white blood cells, platelets, plasma) into predetermined blood components, reducing the burden on the operator compared to conventional manual separation operations. Can be done. - Separation stand function, blood component interface detection function, clamp function, weight measurement function (
It is made more compact by incorporating features such as a tube seal function.

Additionally, experience and intuition were required to detect the interface between blood components and to control the amount (weight) of each blood component, but with this device, anyone can separate blood components into uniform blood components. can be expected.

[Effects of the Invention] As described above, according to the present invention, blood components can be automatically separated using a compact device, and blood components can be separated into desired amounts without requiring experience or intuition.

[Brief explanation of the drawing]

Figures 1 (A) to (C) are external views of one embodiment of the present invention, Figure 2 is a block configuration diagram of this embodiment, and Figures 3 (A) and (B) are blood of this embodiment. First component separation
A flowchart of the sequence, FIG. 4 is a diagram showing the state of a blood bag that has been subjected to strong centrifugation, and FIG.
Figure 6 is a flowchart of the second sequence of this embodiment, Figure 7 is a flowchart of the third sequence of this embodiment, and Figure 8 is a diagram showing the state of a child bag after mild centrifugation. It is an external view of a component separation device. In the figure, 11.21... Pressure plate, 23... Measurement plate, 25... Adjustment handle, 26... Worm gear, 27... Pinion gear, 29... Photo sensor, 31.32... ...Hanger, 33-35...Clamp valve, 36...Select switch, 37...
Start switch, 38... End switch, 41.
42...Handle, 51...Blood bag, 52.53.
...child bag, 54...grandchild bag, 61...concentrated red blood cell layer, 62...white blood cell layer, 63...platelet layer, 6
4... Plasma layer, 65... Liquid platelet layer, 100...
・Control unit, 101... Pressure cylinder control unit, 102・
...Pressure cylinder, 103...Hanger weight conversion section, 1
04... Hanger weight measurement section, 105, 108... Rotary encoder, 106... Weight conversion section, 107.
...Weight measurement section, 110-112...Valve control section,
113... RAM, 114-- Program cassette control section, 115... Connector section, 116... Sound output control section, 117... Speaker, 118... Start lamp, 200... Program cassette It is. Patent applicant Terumo Corporation agent Patent attorney 4 □ 1° ゛1゜Figure 1 (B) Figure 1 (C)

Claims (3)

[Claims] (1) A blood bag pressurizing means that holds a centrifuged blood bag and pressurizes the blood bag at a predetermined pressure, and a blood bag pressurizing means that holds at least two separation bags that communicate with the blood bag via a tube, and that holds and pressurizes the blood bag at a predetermined pressure. a holding and measuring means for measuring bag weight; a regulating means disposed in the middle of a tube between the blood bag and each of the separation bags and regulating the amount of liquid movement within the tube; and measurement weight information of the holding and measuring means. A blood separation apparatus comprising: a control means for inputting as one of the control information and controlling the regulation means. (2) The tube extending from the blood bag branches into a tube that communicates with the first separation bag and a tube that communicates with the second separation bag, and a regulating means is arranged between the branch point and each separation bag. A blood separation device according to claim 1, characterized in that the blood separation device is provided with: (3) The blood separation device according to claim 1 or 2, wherein at least one of the bag holding means for holding the separation bag is located above the blood bag pressurizing means.