JPS63103971A - Filter body holder - Google Patents

Abstract

PURPOSE:To obtain a filter body holder which processes a large amount of material speedily by setting the capacity of the injection hole of a clamping body larger than the capacity of a suction hole set by a check valve. CONSTITUTION:A filter body 20 is sandwiched on the filter body holder 1 and the material isolated by being pumped by a syringe is dispensed into the injection hole together with a solution. The holder 1 is sent to a chemical treating device as a next process after the solution dispensation and the solution is sucked by a sucking mechanism to catch the material on the filter surface 21 of the filter body 20. Then, a reagent is dispensed in the hole 11 and then the dispensed reagent is filtered through a filter surface 21 and dripped gradually in the suction hole 7. The dripped reagent is inhibited by the check valve 3 from flowing and stays in the hole 7. The capacity of the hole 11 is set larger than the capacity of the hole 7 set by the valve 3 to inject a necessary amount of the reagent at a time, and also dip a material on the filter surface 21 in the reagent and perform sufficient treatment.

Description

Detailed Description of the Invention [Field of Industrial Application] The present invention is applicable to pumping dispensing processing, capturing processing, and staining processing in which materials such as cells are sucked and discharged with a syringe, dispersed, and poured onto a filter surface. The present invention relates to a filter body holder that tightly holds a filter body used in preparing a preparation by performing a transfer enclosing process, etc., and enables the above-mentioned process.

[Conventional technology]

To prepare materials such as cells in a solution, first isolate materials such as assembled cells by pumping, capture them with a filter, secondly apply staining etc. to this captured material, and finally slide. Transfer and enclose it on a mirror inspection plate such as glass. Transfer encapsulation here refers to transferring and encapsulating the material captured on the filter surface into a mounting medium or the like on a slide glass.

Conventionally, these steps are often performed one by one manually. Especially when moving from one step to the next, for example from a capture step to a dyeing step.

It is a laborious manual process. Traditional acquisition is 9
For example, a filter is installed in a cylindrical trapping device, and after a solution containing the material is filled onto the filter, the solution is filtered by suction or pressurization, and the material is captured on the filter.

From this capturing step to the next dyeing step, the filter is manually removed from the capturing device and attached to the dyeing device by hand as well.

In addition, when a material is captured by a filter, usually only one filtering surface is formed, so that not only the capture process but also all the processes up to the transfer and encapsulation process take time.

[Problem that the invention seeks to solve]

When the material is a living thing such as a cell, it is necessary to prepare it before it changes, but with conventional technology, which uses a single filtration surface and is mainly manually processed, the throughput is limited. There are limits to this, and it is conceivable that the cells may deteriorate, making it impossible to prepare preparations in a desirable state.

For these reasons, there has been a strong desire for an apparatus that can automate preparation of preparations and rapidly process large amounts of materials.

The present invention is intended to satisfy this demand.

The present invention provides a builter integral holder that is necessary when processing a large amount of material quickly.

[Means to solve the problem]

Therefore, the present invention is composed of nine main bodies 2, a check valve 3, a clamping body 4, and a holding mechanism 5.

The main body 2 has an upper surface as a mounting surface 6 on which the filter body 20 is mounted, and a position facing the filtration surface 21 of the filter body 20 where the solution, chemical treatment liquid, etc. on the filtration surface 21 is sucked. A suction hole 7 was drilled through which the solution passes through when the solution is removed, the upper opening of which is in close contact with the filter body 20, and the lower opening of which is in close contact with the suction mechanism 26 that sucks the solution and the like.

The check valve 3 prevents the solution etc. from flowing down naturally,
It was tightly fitted into the suction hole 7 and assembled.

The holding body 4 has a lower surface that connects the filter body 20 to the main body 2.
A pressing surface 10 that presses against the upper surface of the filter body 20 and a hole through which a solution containing a material, a dyeing solution, etc. are injected at a position facing the filtering surface 21 of the filter body 20, and the lower opening is in close contact with the filter body 20. An injection hole 11 was bored.

Then, the holding mechanism 5 holds the main body 2 and the clamping body 4 between the filter body 20 and the clamping body 4.
The assembly is provided to hold the parts tightly together to the extent necessary.

Further, the volume of the injection hole 11 of the clamping body 4 was set larger than the volume of the suction hole 7 which is set when the check valve 3 is closed.

[Effect]

The filter body holder 1 of the present invention will be explained with reference to FIG. 1, 2, 3 and 4 show an embodiment of the filter body holder 1 according to the present invention,
FIG. 5 shows an apparatus that uses the filter body holder 1 as one of its constituent elements and automatically creates a large amount of preparations. This preparation preparation device 22 includes a pumping dispensing device 23, a reagent processing device 24. And transfer enclosing device 2
It consists of 5.

The filter body 20 is tightly held between the filter body holder l of the present invention having the above-described structure, and is first placed in a panning device. Here, the material pumped and isolated by a syringe is dispensed into the injection hole 11 along with a solution by the syringe.

After the solution is injected, the filter body holder 1 is automatically sent into the chemical processing device 24 which is the first step.

In the chemical processing device 24, the solution is first sucked by the suction mechanism 26, and the material is captured on the filter surface 21 of the filter body 20. From the state in which the material is captured.

Next, reagents such as methanol, staining solution, and washing solution are sequentially dispensed into the injection hole 11 . The reagent dispensed into the injection hole 11 passes through the filter surface 21 of the filter body 20 and gradually drips into the suction hole 7. The flow of the dropped reagent is blocked by the check valve 3 and accumulates in the suction hole 7. The volume of the injection hole 11 is set by the suction hole 7 by the check valve 3.
Since the volume is set larger than the volume of the reagent, it is possible to inject as much reagent as necessary at once, and also to immerse the material on the filtration surface 21 in the reagent, allowing sufficient treatment to take place. Once the material has been sufficiently treated with the reagent.

The reagent is sucked and discharged by the suction mechanism 26. Staining, etc. can be achieved by repeating these processes sequentially.

The necessary reagent treatments are accomplished.

After the above reagent treatment is completed, the filter body holder 1 is first sent into the transfer enclosing device 25.

Here, the holding v1 structure 5 that holds the main body 2 and the clamping body 4 is unassembled, and the filter body 20 is removed from the filter body holder l. The material on the filter surface 2 of the filter body 20 is the slide glass 27.
The preparation is completed by transferring and enclosing it onto a microscopic examination plate such as a microscope, etc., and covering it with a cover glass.

〔Example〕

An embodiment of the filter body holder 1 according to the present invention will be described with reference to FIG.

The main body 2 suction hole 7 and the sandwiching body 4 injection hole 11 are not attached to one, but are attached to the filter surface 21 of the filter body 20.
If there are a plurality of filters, a plurality of filters can be formed at locations facing the filter surface 21. It is advantageous to form a plurality of them because the processing capacity is greatly improved.

If a positioning portion 16 is formed on the main body mounting surface 6 for positioning when the filter body 20 is mounted,
It is convenient because the a position can be easily performed. In the illustrated embodiment, a single step portion is formed to serve as the alignment portion 16.

When the main body 2 and the clamping body 4 are hingedly connected by the hinge connecting mechanism 28, handling becomes easy. In case of the illustrated embodiment 1
A shaft pin 17 is provided at the rear of the main body 2, and the shaft pin 17 is coupled to the rear of the clamping body 4.

When the main body 2 and the clamping body 4 are hinged together via the shaft pin 17 as described above, when the main body 2 and the clamping body 4 are reversed, the lower surface of the main body 2 and the upper surface of the clamping body 4 are flush with each other. When set so as to be 2, the stability of the filter body holder 1 itself is improved, and for example, handling in the transfer enclosing device 25 becomes easy.

A notch on which a slide glass 27 can be mounted is formed on the hinge joint side of the main body 2 to form a slide glass mounting part 19, and the filter mounted on the main body 2 is placed in this mounting part 19. Place the slide glass 27 on which the material of the body filtration surface 21 is to be transferred and encapsulated.

Slide glass 2 with the material displayed in advance
7, it is possible to transfer and enclose it without fail.

There are various ways to place the slide glass 27 on the mounting section 19, such as simply placing it on the mounting section 19, or forming a groove and engaging the slide glass 27 with the groove.

In the illustrated embodiment, a slide glass 27 is provided on the side of the 9-body mounting portion 19 from the shaft pin 17 that connects the 2 main bodies 2 and the clamping body 4 with a hinge.
protrudes a locking pin 18 that locks and prevents natural flow from coming out from the mounting portion 19. By doing this, M
It is possible to simply load and take out the vehicle, and by simply setting the shaft pin 17 slightly longer, that part can be replaced with the locking pin 1.
8, which is advantageous in terms of manufacturing.

The upper end of the check valve 3 that is tightly fitted into the suction hole 7 of the main body 2 is slightly protruded from the mounting surface 6 of the main body 2, and the filter body 2
If the elastic contact portion 8 is in tight elastic contact with the liquid, there will be no leakage of the solution, etc., and suction, etc. can be reliably achieved.

In the two illustrated embodiments, the elastic contact portion 8 is formed by tightly fitting a rubber cylinder.

If the lower opening of the main body suction hole 7 is made into a suction part 9 that comes into close elastic contact with the suction mechanism 26 that suctions the solution etc. in the suction hole 7,
Similar to the above, suction etc. can be achieved reliably. Here, too, a rubber cylinder is tightly fitted to form the suction portion 9.

Also, the lower end of the clamping body injection hole 11 is connected to the clamping body pressing surface 10.
By making the close contact portion 12 protrude slightly more and come into close elastic contact with the filter body 20, the same effects as described above can be obtained. Here, too, a rubber cylinder is used.

The holding mechanism 5 assembles the main body 2 and the clamping body 4.

Here, the shaft rod 141 bullet material 15. It is constructed by combining pressing pieces 13. Various means can be considered for this holding mechanism 5, including the use of the various members described above, as well as the use of magnetic force or the like.

〔Effect of the invention〕

As described above, when the filter body holder according to the present invention is used as a part of the preparation device shown in the embodiment, it can be passed through each device while holding the filter body to accomplish various treatments.

This makes it possible to automate the creation of slides.

During the above processing, the present invention uses a check valve, so it is possible to prevent the natural flow of the staining solution, etc.

It is possible to subject the material to various treatments such as sufficient dyeing treatments.

As a result, if the filter body holder according to the present invention is used, a large amount of material can be easily and reliably processed in a short period of time, which is an excellent effect.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a front half-sectional view showing an embodiment of the present invention. FIG. 2 is a plan view, FIG. 3 is a side sectional view, FIG. 4 is a side view in a fully opened state, and FIG. 5 shows a preparation device incorporating the present invention. Explanation of symbols 1: Filter body holder, 2: Main body. 3: Check valve, 4: Holding body, 5: Holding mechanism. 6: Placement surface, 7: Suction hole, 8: Elastic contact part. 9: Adsorption part, 10: Pressing surface, 11: Injection hole. 12: Close contact part, 13: Pressing piece, 14: Axial rod. 15: Bullet material, 16: Positioning part, 17 Biaxial pin. 18: Locking bin, 19: Mounting section. 20: filter body, 21: filtration surface. 22 nibble ballad creation device. 23 Nipanbing dispensing device, 24: Chemical processing device. 25: Transfer enclosing device, 26: Suction mechanism. 27: Slide glass 27. 28: Hinge connection mechanism. layer

Claims (16)

[Claims] (1) Pumping and dispensing treatment for materials such as cells, capture treatment,
Filter body (20) used to create preparations through reagent treatment such as staining, transfer encapsulation treatment, etc.
A filter body holder (1) that tightly holds the filter body, the upper surface of which is a mounting surface (6) on which the filter body (20) is placed.
), and the filter surface (2) of the filter body (20).
1) is a hole through which the solution on the filtration surface (21) and various reagents for staining are sucked, and whose upper opening is tightly connected to the filter body (20). A main body (2) having a suction hole (7) in close contact with a suction mechanism (26) whose lower opening is in close contact with a suction mechanism (26) that suctions the solution, reagent, etc.; a check valve (3) that is fitted and assembled to prevent the natural flow of the solution, reagent, etc., and a lower surface serving as a pressing surface (10) that presses the filter body (20) against the upper surface of the main body (2); The filter body filtration surface (
A hole in which a solution or reagent containing a material is injected is located at a position opposite to the filter body (21), and the lower opening is located opposite to the filter body (20).
) with an injection hole (11) in close contact with the clamping body (4).
), the main body (2) and the sandwiching body (4), the main body (2)
and a holding mechanism (5) for assembling and holding the filter body (20) to a degree necessary to tightly sandwich the filter body (20) between the holding body (4) and the holding body (4).
), and the volume of the clamping body injection hole (11) is equal to the volume of the check valve (3).
The filter body holder (1) is set to be larger than the volume of the suction hole (7) set in the closed state. (2) The main body suction hole (7) and the sandwiching body injection hole (
The filter body holder (1) according to claim (1), wherein a plurality of 11) are formed at locations facing the filter body filtration surface (21). (3) The filter body (2) is placed on the main body mounting surface (6).
The positioning part (1) is used for positioning when placing the
6) The filter body holder (1) according to claim (1). (4) The filter body according to claim (1), which is formed by forming a cutout portion on which the slide glass (27) is mounted on the main body (2) to serve as the slide glass (27) mounting portion (19). Holder (1). (5) The upper end of the check valve (3) fitted in the main body suction hole (7) is made to slightly protrude from the main body mounting surface (6), so that it tightly presses against the filter body (20). The elastic contact part (8
) A filter body holder (1) according to claim (1). (6) The lower opening of the main body suction hole (7) is configured as a suction part (9) that comes into close elastic contact with a suction mechanism (26) that sucks the solution, etc. in the suction hole (7). The filter body holder (1) according to (1). (7) The lower end of the sandwiching body injection hole (11) slightly protrudes from the sandwiching body pressing surface (10), and the filter body (20)
) The filter body holder (1) according to claim (1), which is formed as a close contact part (12) in close elastic contact with the filter body holder (1). (8) Pumping and dispensing treatment for materials such as cells, capture treatment,
Filter body (20) used to create slides after various treatments associated with staining, transfer and encapsulation, etc.
A filter body holder (1) that tightly holds the filter body, the upper surface of which is a mounting surface (6) on which the filter body (20) is placed.
), and the filter surface (2) of the filter body (20).
1) is a hole through which the solution on the filtration surface (21) and various reagents for staining are sucked, and whose upper opening is tightly connected to the filter body (20). A main body (2) having a suction hole (7) in close contact with a suction mechanism (26) whose lower opening is in close contact with a suction mechanism (26) that suctions the solution, reagent, etc.; a check valve (3) that is fitted and assembled to prevent the natural flow of the solution, reagent, etc., and a lower surface serving as a pressing surface (10) that presses the filter body (20) against the upper surface of the main body (2); The filter body filtration surface (
A hole in which a solution or reagent containing a material is injected is located at a position opposite to the filter body (21), and the lower opening is located opposite to the filter body (20).
) with an injection hole (11) in close contact with the clamping body (4).
), the main body (2) and the sandwiching body (4), the main body (2)
and a holding mechanism (5) for assembling and holding the filter body (20) to a degree necessary to tightly sandwich the filter body (20) between the holding body (4) and the holding body (4).
), and a shaft pin (17) connects the main body (2) and the holding body (4).
a hinge coupling mechanism (28) that is hinge-coupled via the check valve (3);
The filter body holder (1) is set to be larger than the volume of the suction hole (7) set in the closed state. (9) The main body suction hole (7) and the sandwiching body injection hole (
The filter body holder (1) according to claim (1), wherein a plurality of 11) are formed at locations facing the filter body filtration surface (21). (10) The filter body (
20) for positioning when placing the
16) A filter body holder (1) according to claim (1). (11) The filter body according to claim (1), wherein a cutout portion for mounting a slide glass (27) is formed in the main body (2) to serve as a slide glass (27) mounting portion (19). Holder (1). (12) The upper end of the check valve (3) fitted in the main body suction hole (7) is made to slightly protrude from the main body mounting surface (6), so that it tightly presses against the filter body (20). The contacting elastic part (
8) A filter body holder (1) according to claim (8). (13) A claim in which the lower opening of the main body suction hole (7) is formed as a suction part (9) that comes into close elastic contact with a suction mechanism (26) that sucks the solution, etc. in the suction hole (7). (8)
The filter body holder (1) described in (1). (14) The lower end of the sandwiching body injection hole (11) slightly protrudes from the sandwiching body pressing surface (10), and the filter body (2
The filter body holder (1) according to claim 8, wherein the filter body holder (1) is formed as a close contact part (12) that comes into close elastic contact with the filter body holder (1). (15) When the main body (2) and the clamping body (4) are reversed, the lower surface of the main body (2) and the upper surface of the clamping body (4) are set to be flush with each other. Filter body holder (1) according to range (8). (16) From the shaft pin (17) to the main body mounting portion (1
According to claim (8), a locking pin (18) is protruded from the slide glass (27) to lock the slide glass (27) and prevent it from coming out naturally from the mounting portion (19). Filter body holder (1).