JPS62100281A - Iodine-containing yeast, production thereof and yeast pharmaceutical containing enriched iodine content - Google Patents

Abstract

PURPOSE:To obtain an iodine-containing yeast suitable as a material for ingesting iodine in a large amount or daily without causing side effect, by ingesting an iodine compound in yeast fungi. CONSTITUTION:The concentration of an iodine compound in a culture medium used for cultivating a yeast fungal group is increased stepwise to select a strain capable of ingesting the iodine, carry out iodine ingesting cultivation of the strain and give the aimed iodine-containing yeast. Yeast fungal bodies must contain at least 1mug, based on 100g dried weight, iodine, preferably at least 0.1mg, based on 100g dry weight, iodine as an analytical result of elementary composition thereof.

Description

[Detailed Description of the Invention] [Field of the Invention] The present invention provides an iodine-containing yeast that can easily ingest iodine in a desirable form, a method for producing the same, and an iodine-containing pickle using the same. Concerning fortified yeast preparations.

[Background of the Invention] Iodine is an essential element for living organisms, and its involvement in thyroid function has been clarified through research to date. Moreover, preparations containing this iodine are manufactured for the purpose of treating various diseases other than thyroid dysfunction.

In general, drugs for ingesting iodine into the body include gastrointestinal 1
Side effects include irritability, iodine rash, and iodine rash. Regarding this problem of side effects, 1. Therefore, a method of ingesting ioS7 as an organic compound such as iodine lecithin has been proposed and implemented. However, iodine is very large,
Alternatively, [1] In order to regularly ingest iodine, materials containing iodine in a more favorable state from a medical or nutritional standpoint are required.

[Summary of the Invention] An object of the present invention is to provide a material that allows iodine to be easily ingested in a preferred form.

Uninvented is at least dry multilayer 1001: per IAL,
It provides yeast i+2 containing g of iodine.

The above yeast can be easily cultivated by gradually reducing the concentration of iodine compounds in the medium used for culturing the yeast f1 group, and by simultaneously selecting an iodine-capable bacterial strain and cultivating that strain for iodine uptake. can be produced.

In addition, the yeast described in L is treated with cell wall destruction and is
Iodine containing fj B1 with excipients or carriers! It can be preferably used as a yeast preparation with positive reinforcement.

[Effects of the invention] (The iodine-containing yeast of the invention allows the fermenting bacteria to ingest all the iodine compounds, so that most of the iodine compounds can be easily ingested into the living body, without the above-mentioned side effects. Therefore, the uninvented iodine-containing yeast I is suitable as a material for ingesting iodine in large quantities or on a daily basis. Since it does not impair the excellent attachment properties of yeast cells, it is also suitable as a material for attachment reinforcement agents that are ingested on a daily basis.Furthermore, it is used for the treatment of specific diseases by administering iodine. In this case, the cell wall-disrupted I-2 yeast t1 can be used as an easy-to-drink preparation suitable for oral administration using an excipient or carrier.

In addition, the method for producing the iodine-containing enzyme V) that does not emit iodine is as follows.

Since it is possible to simultaneously select an iodine-ingesting bacterial strain and cultivate the strain to ingest iodine, the above-mentioned production of the iodine-containing yeast can be easily carried out.

[Detailed Description of the Invention I NfFzJ bacteria can be used not only for the production of fermented foods, but also for the excellent use of yeast cells! In addition to its properties (especially protein and vitamins), it has applications as a nutritional supplement, or as food and feed. However, in such applications where yeast nl carriers are directly ingested, few attempts have been made to actively enhance specific components of the bacterial cells.

According to research by the inventor, iodine-resistant strains exist as natural mutant strains with a certain frequency in the yeast fE) group commonly used in the fermentation industry. In addition, these iodine-resistant strains also have an ability to uptake iodine. Therefore, the io-1 used in the practice of the present invention
Ingestive strains can be easily selected and used from commonly used yeast strains.

There are no particular restrictions on the type of yeast that can be used in the uninvented process, and the types of bacteria that are used in the fermentation process can be used. However, considering that it is easy to enter and that research is progressing, yeast belonging to the genus Saecharamyces,
In particular, Saccharomyces cerevisiae (Saccharo)
myces gerevisiae) is preferably used.

The iodine-containing yeast of the present invention contains at least Igg per 100 g of dry weight (preferably at least 0.1 mg per 100 g of dry weight) as a result of analysis of the elemental composition of the bacterial cells.
It is characterized by containing iodine. LkiPIJ
When used as a material for a drug that targets the mother by aggressive iodine injection, the dry weight of at least 10
Preferably, it contains 0.1 mg of iodine per 0 g. However, dry weight 100g dust Igg to 10m
Yeast containing g of iodine in its bacterial cells can also be preferably used as a nutritional supplement for iodine deficiency, or as a material for drugs that treat 'N therapy with a small amount of iodine film. I can do it.

The iodine uptake bacterial strains used in conducting the comparison are:
As mentioned above, the naturally variable U strain can be selected from the bacterial group whose traits are relatively unfixed. That is, by culturing the yeast group II in a medium containing a high concentration of iodine compounds, it is possible to select iodine-X uptake strains.

By using the iodine uptake bacterial strain obtained as described in Section I, it is possible to ingest a high concentration of iodine into the yeast cells. It is also possible to create strains with even better collagen uptake and other properties through artificial mutation using radiation, drugs, etc.

Furthermore, according to the yeast production method of the present invention, f'l! Jul
iodine in the medium used in the j′8 cultivation of the bacterial group,
By decreasing the compound concentration stepwise, it is also possible to select an iodine-accommodating bacterial strain and to culture that strain at the same time. A method to gradually lower the iodine concentration in 18m is to prepare multiple media with different iodine compound concentrations and sequentially transplant yeast groups to the medium with higher iodine compound concentration. Efficient in strain selection. Alternatively, it is also possible to use a method of gradually increasing the iodine compound concentration by gradually adding the iodine compound to the liquid medium.

There are no particular restrictions on the medium used in the selection and cultivation of L yeast, including the production method of yeast ENI of the present invention.
11! ! However, a complex medium such as the one used in the Examples described later may be used. Furthermore, since there are no specific restrictions on the culture conditions (pH, temperature 'v), it is preferable to carry out the cultivation under the optimal conditions for the fermentation ITJ used. Although it depends on the type of yeast, it is generally preferable to carry out the process under aerobic conditions in terms of production efficiency.

In selecting and culturing L yeast, it is often preferable to use a metal salt of iodine as the iodine compound added to the medium. Particularly preferred metal salts of iodine include potassium iodide and sodium iodide.

When potassium iodide or sodium iodide is used, the iodine concentration of the medium used in the method for producing yeast fNt of the present invention is preferably about 0.001% at the culture start level. Also.

The degree of potassium iodide or I.lium iodide of the medium at the end of cultivation is determined depending on the final iodine content per dry weight, but it is relatively high. If the purpose is to produce bacterial cells containing ￥ (dry ball r1′jt o o g ”-isl Omg h),
Yul-2 fermentation L31 is preferably 1.0% or less
If the selection of iodine uptake bacterial strains is set to [-1, culture media with different iodine concentrations, 1!
For about 12 to about 24 hours each time, and about 20 to about 20 to about 20 to about 20 to It is preferable to carry out the treatment for about 24 hours.

The fermentation enzyme (IT) of the present invention produced as described above can be used in various ways. Generally, when the purpose is to ingest yeast cells, other methods of cell treatment include physically or chemically destroying the cell walls that impede digestion and absorption. It can be prepared in various forms depending on the purpose of iodine intake, etc. For example, known yeast preparations (beer fermentation 1
Preparation method of preparation '9) and food production (protein production)
This method can be carried out by referring to the method for treating bacterial cells for the purpose.

In particular, if the iodine membrane goo, which is a two-day medical treatment, is used as a first medical treatment,
It is preferable to prepare the cell wall-disrupted L2 yeast together with excipients or carriers in the form of tablets or capsules.When preparing as described above, the yeast is generally subjected to a dry φ powder treatment.

As mentioned above, the iodine-containing balm-enriched preparation of the present invention prepared in this way is particularly excellent for oral administration.

Examples of the present invention are shown below.

[Example 1] Potassium iodide was added to a medium having the composition shown below.
ooi% (I), O, 01% (IN), 0.05% (
m) and 01% (IV) were added.

Fermentation I: Jl modified medium Ml composition (per book 12) Glucose
10g peptone
5g powdered yeast extract 3g powdered malt extract The pH was adjusted to 5.6 using 3gIN hydrochloric acid.

Saeeharniyces Cer
evisiae) was cultured in medium (I) with the lowest potassium iodide concentration at 27°C for 24 hours,
Next, the cells were transplanted to medium (Il), picked up in the same manner, and transplanted and cultured to medium (m) and (IV) sequentially.

In culture in the last (rV) medium, dry ton i
A strain with an iodine uptake of 10 mg-L or more per 100 g of i was obtained. This strain was continuously cultured for 24 hours in the medium (■) above to ensure sufficient intake of iodine. The analysis results of the obtained iodine-containing yeast are shown below.

Iodine 18, 3mg per 100g dry weight (11
Analysis results by the Food Research Center (based on iodine titration method) l-2 The iodine-containing yeast according to the present invention was powdered and put into capsules to prepare a yeast preparation.

When 1 to 3 g of the above yeast preparation was taken 1 to 3 times a day, no side effects were observed.

Claims (1)

[Scope of Claims] 1. Yeast containing at least 1 μg of iodine per 100 g of dry weight. 2. Claim 1, characterized in that it contains at least 0.1 mg of iodine per 100 g of dry weight.
Yeast as described in section. 3. The yeast according to claim 2, which contains at least 10 mg of iodine per 100 g of dry weight. 4. The yeast according to claim 1, wherein the yeast belongs to the genus Saccharomyces. 5. The yeast according to claim 4, wherein the yeast is Saccharomyces cerevisiae. 6. At least per 100 g of dry weight, characterized in that the selection of an iodine-capable bacterial strain and the iodine-capturing culture of the strain are simultaneously carried out by increasing the concentration of iodine compounds in the medium used in the culture of the yeast group in stages. A method for producing yeast containing 1 μg of iodine. 7. The method is characterized in that the iodine concentration in the medium used is gradually increased by preparing a plurality of mediums with different concentrations of iodine compounds and sequentially transplanting the yeast bacteria to the medium with higher concentrations of iodine compounds. A method for producing yeast according to claim 6. 8. The method for producing yeast according to claim 6, wherein the iodine compound is potassium iodide. 9. A patent characterized in that the potassium iodide concentration of the medium at the start of the culture is 0.001% or less, and the potassium iodide concentration of the medium at the end of the culture is 1.0% or more A method for producing yeast according to claim 8. 10. An iodine-enriched yeast formulation containing cell wall-disrupted yeast containing at least 1 μg of iodine per 100 g of dry weight together with an excipient or carrier. 11. The yeast preparation according to claim 10, wherein the yeast has been subjected to drying and powdering treatment. 12. The yeast preparation according to claim 10, which is for oral administration.