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JPS6113158A - Biochemical analyser - Google Patents

Biochemical analyser

Info

Publication number
JPS6113158A
JPS6113158A JP13399584A JP13399584A JPS6113158A JP S6113158 A JPS6113158 A JP S6113158A JP 13399584 A JP13399584 A JP 13399584A JP 13399584 A JP13399584 A JP 13399584A JP S6113158 A JPS6113158 A JP S6113158A
Authority
JP
Japan
Prior art keywords
serum
whole blood
main body
photometric
operated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP13399584A
Other languages
Japanese (ja)
Other versions
JPH0439624B2 (en
Inventor
Tsuneo Narushima
鳴島 恒雄
Masashi Azuma
我妻 将士
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Konica Minolta Inc
Original Assignee
Konica Minolta Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Konica Minolta Inc filed Critical Konica Minolta Inc
Priority to JP13399584A priority Critical patent/JPS6113158A/en
Publication of JPS6113158A publication Critical patent/JPS6113158A/en
Publication of JPH0439624B2 publication Critical patent/JPH0439624B2/ja
Granted legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B11/00Feeding, charging, or discharging bowls
    • B04B11/04Periodical feeding or discharging; Control arrangements therefor
    • B04B2011/046Loading, unloading, manipulating sample containers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099Characterised by type of test elements
    • G01N2035/00138Slides
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346Heating or cooling arrangements
    • G01N2035/00356Holding samples at elevated temperature (incubation)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465Separating and mixing arrangements
    • G01N2035/00495Centrifuges

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

PURPOSE:To attain to enhance the efficiency of analytical work, by providing a centrifugal separator for separating serum from whole blood, a thermostatic tank having a photometric head and a pipet apparatus. CONSTITUTION:Centrifugal separators 2 each separating serum from whole blood, a tunnel shaped thermostatic tank 20 having a plurality of photometric heads and a pipe apparatus 54 capable of sucking separated serum to distribute the same to analytical slides passing through the thermostatic tank 20 are provided in a main body 1. Test tubes 8 each receiving a proper amount of whole blood are mounted to the disc 6 of each centrifugal separator 2, which are positioned directly under circular holes 11 provided to the main body 1, through said holes 11. Next, when an operation board 80 is manipulated, a driving part 3 is operated and the output shaft 4 thereof is rotated by 1/3. At this position, a rotation energizing means 12 is operated and whole blood in each test tube is separated into blood corpuscles and serum on the basis of specific- gravity difference thereof by centrifugal force due to the high speed rotation of each disc 6. Next, the shaft 4 is further rotated by 1/3 and the pipet apparatus 54 is operated to suck a proper amount of the serum in each tube 8. This sucked serum is dripped on the measuring surface of each analytical slide and irradiated with photometric light while the reflected light thereof is operated by a light receiving element and a measured value is displayed on a display device 76.

Description

【発明の詳細な説明】 〔産業上の利用分野〕 この発明は生化学分析装置、詳しくは反応試薬が含浸さ
れた分析素子を備えた分析スライドに被検サンプルを供
給し、該被検サンプルとの反応により色の濃度変化等を
測定して化学的に分析するための装置に関するものであ
る。
[Detailed Description of the Invention] [Industrial Application Field] This invention supplies a test sample to a biochemical analyzer, specifically an analysis slide equipped with an analysis element impregnated with a reaction reagent, and This invention relates to a device for chemically analyzing changes in color density by measuring changes in color density due to reactions.

〔従来の技術〕[Conventional technology]

一般に、血液中に特定成分が含有されているか否か、そ
の含肴量等を知る場合に反応試薬が含浸された分析素子
を備えた分析スライドを用い、この分析素子に分析すべ
き被検サンプルを滴下しで供給し、これを反応用恒温槽
内において被検サンプルと反応せしめ、その反応の進行
状態または結果を1例えば反応による色の濃度変化を光
学式濃度測定器により測定する手段。
Generally, when determining whether or not a specific component is contained in blood and its content, an analysis slide equipped with an analytical element impregnated with a reaction reagent is used, and the test sample to be analyzed is placed on this analytical element. Means for supplying the solution dropwise, reacting it with a test sample in a thermostatic reaction chamber, and measuring the progress or result of the reaction (1), for example, the change in color density due to the reaction, using an optical density meter.

その他の手段により測定分析する装置が知られていた しかしながら、上記測定に被検サンプルとして使用され
る血清は予め別工程で遠心分離装置等を用いて全血より
分離し、ピペットにて分析スライドに個々に手動にて供
給しなければならず、その取扱が面倒であった。
Devices that perform measurement and analysis using other means have been known.However, the serum used as the test sample for the above measurements is separated from whole blood in a separate process using a centrifugal separator, etc., and then transferred to an analysis slide using a pipette. They had to be fed individually, which was troublesome to handle.

〔発明の目的〕[Purpose of the invention]

この発明は上記の問題を解消するためのもので、全血か
ら被検サンプルとしての血清分離。
This invention is intended to solve the above problems, and involves separating serum as a test sample from whole blood.

分析スライドへの分離血清の自動供給及び測定が一気に
できるようにした生化学分析装置を提供することを目的
としている。
The object of the present invention is to provide a biochemical analyzer that can automatically supply and measure separated serum to an analysis slide at the same time.

〔発明の構成〕[Structure of the invention]

この発明は全血より血清を分離する遠心分離手段と1分
析スライドを直線的に一定のピッチで搬送する手段を有
し、かつ分析スライドの停止位置に複数個の測光ヘッド
を有するトンネル型の恒温槽と1分離血清を吸引し、恒
温槽内を通る分析スライドに分注できるピペット装置と
を備えることにより、上記目的を達成できるようにした
ものである。
This invention is a tunnel-type thermostat that has a centrifugal separation means for separating serum from whole blood, a means for transporting one analysis slide linearly at a constant pitch, and a plurality of photometric heads at the stop position of the analysis slide. The above objective can be achieved by being equipped with a bath and a pipette device that can aspirate one separated serum and dispense it onto an analysis slide that passes through a constant temperature bath.

〔実施例〕〔Example〕

次に、この発明を添付図面に示す一実施例にもとづいて
説明する。
Next, the present invention will be described based on an embodiment shown in the accompanying drawings.

1は生化学分析装置本体(以下、単に本体という)で、
該本体1内には全血より血清を分離するための遠心分離
手段2が設けられている。
1 is the biochemical analyzer main body (hereinafter simply referred to as the main body),
A centrifugal separator 2 is provided within the main body 1 for separating serum from whole blood.

遠心分離装置2は第2図乃至第4図に示すように駆動部
3の出力軸4の上端部に三方向に延びる同長の腕体5を
設け、該腕体5の各先端部にはディスク6がその支軸6
′を介して回転自在に軸支されている。該ディスク6は
有底円筒体6aに上面板6bを固着してなる。各ディス
ク6の上面板6bの外縁部には複数の穴7.7−が同一
円上に等配列膜され、法人7,7−・−にはそれぞれ試
験管8を受領できる筒状容器9が第3図(I)に″示す
ようにピン10を介して揺動自在に垂下され、ディスク
6の高速回転時の遠心力でその下端部が外方に向は同図
(Ir)に示すように揺動できるようになっている。前
記ディスク6は駆動部3の作動により矢印方向に3分の
1回転(120度)・づつ回転する出力軸4とともに同
一円内を公転し、定位置停止手段(図示せず)により予
め定められた位置A、B、Cにて正確に停止できるよう
になっている。
As shown in FIGS. 2 to 4, the centrifugal separator 2 is provided with an arm 5 having the same length extending in three directions at the upper end of the output shaft 4 of the drive unit 3. The disk 6 is its spindle 6
It is rotatably supported via . The disk 6 is formed by fixing a top plate 6b to a bottomed cylindrical body 6a. A plurality of holes 7, 7- are arranged equally on the same circle on the outer edge of the top plate 6b of each disk 6, and each of the corporations 7, 7-, . . . has a cylindrical container 9 that can receive a test tube 8. As shown in FIG. 3 (I), it is suspended swingably through the pin 10, and due to the centrifugal force when the disk 6 rotates at high speed, its lower end is directed outward as shown in the same figure (Ir). The disk 6 revolves in the same circle together with the output shaft 4, which rotates one-third of a turn (120 degrees) in the direction of the arrow by the operation of the drive unit 3, and stops at a fixed position. It is possible to accurately stop at predetermined positions A, B, and C by means (not shown).

11はディスクの停止位置Aに対応する本体1の一上面
板1aに設けた円孔で、該円孔11はディスク上面の穴
7−・の全部が完全に露出できる大きさになっている。
Reference numeral 11 denotes a circular hole provided in one top plate 1a of the main body 1 corresponding to the stop position A of the disk, and the circular hole 11 is large enough to completely expose the entire hole 7-. on the top surface of the disk.

これは試験管8をディスク6の穴7に垂下した容器9に
装着するためのものである。12はディスクの停止位置
Bにあるディスクを高速回転させるための回転付勢手段
で、該回転付勢手段12は第4図に示すようにソレノイ
ド13の作動により一端を支点として上下動できる可動
板14上にモータ15を設置し、該モータ15の錐軸1
6に上向き凹状の摩擦部材17を備え、可動板14の上
下動で摩擦部材17がディスク6の支軸6′の下端に設
けた下向き凸状の摩擦部材18に結合、離反できるよう
に構成されている。この回転付勢手段12のモータ15
は試験管8内の全血より血清を分離するに必要な回転数
(例え4f3000rpm以上)の高速回転が得られる
ものが使用されることは勿論である。19はディスクの
停止位置Cに対応する本体1の上−板1aに設けたピペ
ット装入孔で、該ピペット装入孔19はディスク上の一
つの穴7 (試験管8の開口部)に合致する。なお、C
位置においてディスク6はB位置の回転付勢手段と同構
造の回転付勢手段(図示せず)にて所定角度正逆回転さ
れ、ピペット装置にて吸引しようとする血清が入った試
験管8が選択できるようになっている。この選択はディ
スクの適所に例えばバーコードを付し、これを検出器(
図示せず)にて検出して行うようにしておけばよい。
This is for attaching the test tube 8 to the container 9 which is suspended in the hole 7 of the disk 6. Reference numeral 12 denotes a rotation urging means for rotating the disk at a high speed at the disk stop position B. The rotation urging means 12, as shown in FIG. A motor 15 is installed on the cone shaft 1 of the motor 15.
6 is provided with an upwardly concave friction member 17, and the friction member 17 is configured to be coupled to and separated from a downwardly convex friction member 18 provided at the lower end of the support shaft 6' of the disk 6 by vertical movement of the movable plate 14. ing. The motor 15 of this rotation urging means 12
Needless to say, a device capable of rotating at a high speed (for example, 4 f 3000 rpm or higher) necessary to separate serum from whole blood in the test tube 8 is used. Reference numeral 19 denotes a pipette insertion hole provided in the upper plate 1a of the main body 1 corresponding to the stop position C of the disk, and the pipette insertion hole 19 matches one hole 7 (opening of the test tube 8) on the disk. do. In addition, C
At the position, the disk 6 is rotated forward and backward at a predetermined angle by a rotation urging means (not shown) having the same structure as the rotation urging means at the B position, and the test tube 8 containing the serum to be aspirated with the pipette device is You can choose. This selection involves attaching, for example, a barcode to a suitable location on the disc and transmitting it to the detector (
(not shown).

20は本体1の上面板1a上に設けた溝1b内に設置さ
れた分析スライド21のトンネル型の恒温槽で、該恒温
槽20は第5図に示すように金属板22で外面を保護さ
れた断熱材23にて形成した断面口型の上部筐体24と
、断面U型の下部筺体°25を両者の開口側が向い合う
ように組合せ、上部筐体24及び下部筐体25内に発熱
体26及び放熱板27を配置してなる。
Reference numeral 20 denotes a tunnel-shaped constant temperature bath for the analysis slide 21 installed in the groove 1b provided on the top plate 1a of the main body 1, and the constant temperature bath 20 has an outer surface protected by a metal plate 22 as shown in FIG. An upper casing 24 with an opening-shaped cross section and a lower casing 25 with a U-shaped cross-section formed of a heat insulating material 23 are combined so that their opening sides face each other, and a heating element is placed inside the upper casing 24 and the lower casing 25. 26 and a heat sink 27 are arranged.

該下部筺体25内の放熱板27は断面u型に成形され、
その上端間には中央部に分析スライド21の両端縁を軽
く把持できる溝28a、’28bが対向した搬送路28
を有する天板29が載置されている。
The heat sink 27 in the lower housing 25 is formed into a U-shaped cross section.
Between the upper ends thereof, there is a conveying path 28 in which opposing grooves 28a and 28b are formed in the center to lightly grip both edges of the analysis slide 21.
A top plate 29 is placed thereon.

30は搬送路28の分析スライド21を一定のピッチで
間歇的に搬送するための間歇搬送手段で、該間歇搬送手
段30は図示のように下部筺体25内の放熱板27の内
底面中央部に路長方向に沿って2条の溝31a、、31
bを設けている。該11iaia、3tbにはH型基台
32を摺動自在に嵌合している。該H型基台32は図示
しない駆動装置により所定のストロークで前後動できる
ようになっている。33はH型基台32の、両側壁32
a、32b間に所定間隔を隔てて渡設した横軸で、該横
軸33の中央部には押圧部材34が起倒自在に取付けら
れている。
Reference numeral 30 denotes an intermittent conveyance means for intermittently conveying the analysis slides 21 on the conveyance path 28 at a constant pitch, and the intermittent conveyance means 30 is provided at the center of the inner bottom surface of the heat dissipation plate 27 in the lower housing 25 as shown in the figure. Two grooves 31a, 31 along the path length direction.
b. An H-shaped base 32 is slidably fitted to the 11iaia and 3tb. The H-shaped base 32 can be moved back and forth with a predetermined stroke by a drive device (not shown). 33 are both side walls 32 of the H-shaped base 32
A pressing member 34 is attached to the center of the horizontal shaft 33, which extends between a and 32b at a predetermined distance.

また、H型基台32の両側壁32a、32bの外側には
図示しない駆動装置(H型基台32の駆動装置と共通の
ものでも、別個のものでもよい)で路長方向に所定のス
トロークにて前後する摺動板35 a、  35 bが
対向している。該摺動板35a、35bには前記横軸3
3の設置間隔と同一間隔で縦長孔36a、36bが設け
られ、該縦長孔36a、36bには押圧部材34の両端
面上部から突出したピン37a、37bが嵌入している
。従って、押圧部材34は前記摺動板35a、35bが
H型基台32を停止させた状態で前方に向けて摺動する
ことにより。
Further, on the outside of both side walls 32a and 32b of the H-shaped base 32, a drive device (not shown) (which may be common to the drive device of the H-shaped base 32 or a separate device) is used to drive a predetermined stroke in the path length direction. Front and rear sliding plates 35a and 35b face each other. The horizontal shaft 3 is attached to the sliding plates 35a and 35b.
Vertical holes 36a, 36b are provided at the same interval as the installation interval of 3, and pins 37a, 37b protruding from the upper portions of both end surfaces of the pressing member 34 are fitted into the vertical holes 36a, 36b. Therefore, the pressing member 34 is slid forward with the sliding plates 35a and 35b stopping the H-shaped base 32.

第6図(1)の如く起立する。逆に後方に向けて摺動す
るときは同図(II)の如く後方に倒れ・るようになっ
ている、この摺動板の動作にH型基台32の摺動を組合
せることにより押圧部材34は起立状態で前進するとと
もに、倒れ状態で後進することができる。そして押圧部
材34は起立時にはその上端突部34aが第7図に示す
ように分析スライド21の搬送路28より上方に突出し
、倒れ時には該搬送路下に没するから、押圧部材34の
前記作動(起立状態での前進及び倒れ状態での後進)を
繰返すことにより分析スライド21は間歇的に順次前方
に向は搬送されることとなる。
Stand up as shown in Figure 6 (1). On the other hand, when sliding backwards, the sliding plate is designed to fall backwards as shown in Figure (II), and by combining the sliding movement of the H-shaped base 32 with the movement of this sliding plate, it is possible to press The member 34 can move forward in an upright state and can move backward in a collapsed state. When the pressing member 34 stands up, its upper end protrusion 34a protrudes above the conveying path 28 of the analysis slide 21 as shown in FIG. 7, and when it falls down, it sinks below the conveying path. By repeating the forward movement in an upright state and the backward movement in a fallen state, the analysis slide 21 is intermittently and sequentially transported forward.

38は前記搬送路28に分析スライド2.1を供給する
供給部で、該供給部38は第8図に示すように分析スラ
イド21を積層収納したカセット39を周縁部上面に着
税自在に配置した回転体40と、該回転体40の支軸4
1の上端に固定した静止床42と、該静止床42上に設
置したプッシャー43とからなる。プッシャー43は第
9図に示すように対向した取付板44a。
Reference numeral 38 denotes a supply unit that supplies the analysis slides 2.1 to the conveyance path 28, and the supply unit 38, as shown in FIG. The rotating body 40 and the spindle 4 of the rotating body 40
1, and a pusher 43 installed on the stationary floor 42. The pusher 43 has mounting plates 44a facing each other as shown in FIG.

44b間に渡設した2条のシャフト45a、45bにプ
ッシャー板46を備えた可動体47を摺動自在に嵌合し
、該可動体47を取付板44aの外方に配置したモータ
48の錐軸に固定した駆動プーリ49と、他方の取付板
44b側に立設した側板44cに軸支された軸に固定し
た従動プーリ50との間に張設したロープ51に係留し
ている。従って、モータ48の作動でロープ51を介し
てプッシャー板46が前進すると。
A movable body 47 equipped with a pusher plate 46 is slidably fitted to two shafts 45a and 45b extending between 44b, and the movable body 47 is arranged outside the mounting plate 44a. It is moored to a rope 51 stretched between a driving pulley 49 fixed to a shaft and a driven pulley 50 fixed to a shaft supported by a side plate 44c erected on the other mounting plate 44b side. Therefore, when the pusher plate 46 moves forward via the rope 51 due to the operation of the motor 48.

その前面に位置するカセット39内の分析スライド21
が図示のように押出され、m送路28に供給されるよう
になっている。この場合において回転体40はその駆動
手段52の作動により回転し、プッシャーFi46の前
面に必要な分析スライド21を装填したカセット39が
来るよう選択できるようになっている。
Analysis slide 21 in the cassette 39 located in front of it
is extruded as shown and supplied to the m feed path 28. In this case, the rotating body 40 is rotated by the operation of its driving means 52, and the cassette 39 loaded with the necessary analysis slides 21 can be selected to be placed in front of the pusher Fi46.

53は前述したように搬送路28内を間歇的に走行する
分析スライド21に分離血清を分注するための分注孔で
、該分注孔53は分析スライド21の停止位置に対応す
る上部筺体24に貫通状に設けられている。54はピペ
ット装置で、該ピペット装置54は前記遠心分離装置2
を構成するディスク6の停止位置Cに対応する本体1の
上面板1aに設けたピペット装入孔19を通して下降し
、試験管8内の血清吸引後、上動し、その上動点にて回
動して前記分注孔53上において下降し、吸引した血清
を分析スライド21の素子面21aに分注できるように
なっている。ピペット装置54は第10図に示すように
本体1の上面板1aに設けた軸受部材55に回転自在に
支持された垂直軸56の頭部に固定した水平腕杆57の
先端コ状枠57aに上下動自在にピペット主体58を保
持するとともに。
Reference numeral 53 denotes a dispensing hole for dispensing separated serum to the analysis slide 21 that travels intermittently within the transport path 28, as described above, and the dispensing hole 53 is located in the upper housing corresponding to the stopping position of the analysis slide 21. 24 in a penetrating manner. 54 is a pipette device, and the pipette device 54 is connected to the centrifugal separator 2.
The pipette descends through the pipette insertion hole 19 provided in the upper surface plate 1a of the main body 1 corresponding to the stop position C of the disc 6 constituting the test tube 8, moves upward after aspirating the serum in the test tube 8, and rotates at the upper moving point. It moves downward over the dispensing hole 53, and the aspirated serum can be dispensed onto the element surface 21a of the analysis slide 21. As shown in FIG. 10, the pipette device 54 is attached to a U-shaped frame 57a at the tip of a horizontal arm rod 57 fixed to the head of a vertical shaft 56 rotatably supported by a bearing member 55 provided on the upper surface plate 1a of the main body 1. It holds the pipette main body 58 so as to be able to move up and down.

該ピペット主体58の一側面に形成したラック59を前
記コ状枠57aの中間部に軸支したピニオン60に噛合
している。ビニオン60は図示しない駆動手段に水平腕
杆57の内部を通して連繋し、その正逆回転でピペット
主体58を上下動できるようになっている。また、前記
垂直軸56は本体1内に設置したモータ61の駆動ギア
62に噛合した従動ギア63に連繋しており、モータ6
1の正逆転により回動し、ピペット主体58が前記ピペ
ット装入孔19及び分注孔53の中心間を回動できるよ
うにしている。
A rack 59 formed on one side of the pipette main body 58 is engaged with a pinion 60 pivotally supported in the middle of the U-shaped frame 57a. The pinion 60 is connected to a drive means (not shown) through the inside of the horizontal arm rod 57, and can move the pipette main body 58 up and down by rotating the pinion in forward and reverse directions. Further, the vertical shaft 56 is connected to a driven gear 63 that meshes with a driving gear 62 of a motor 61 installed inside the main body 1.
The pipette main body 58 can rotate between the centers of the pipette insertion hole 19 and the dispensing hole 53.

64は吸引機、65は吸引機64に連繋した導管である
64 is a suction machine, and 65 is a conduit connected to the suction machine 64.

66は測光用光学手段で、該測光用光学手段66は第8
図、第11図及び第12図に示すように、ハロゲンラン
プ等の光源67より発生した光線をレンズ68及びフィ
ルター69を介して所望する波長の測光光線にし、該測
光光線は回動ミラー装置70を介して分配され、光ファ
イハーフ1を通して分析スライド21の測定面(素子裏
面)に近接させた測光ヘッド72に誘導され照射される
。この照射光は測定面から反射して光ファイバー73を
通して受光素子74に伝送され、マイクロコンピュタ−
等の演算装置75により演算され、その測定値を本体1
の表示窓76に表示できるようになっている。この測定
値は必要に応じてロール状記録紙(図示せず)に印字さ
れるようにしてもよい。
66 is an optical means for photometry, and the optical means 66 for photometry is the eighth
11 and 12, a light beam generated from a light source 67 such as a halogen lamp is converted into a photometric beam of a desired wavelength through a lens 68 and a filter 69, and the photometric beam is transmitted to a rotating mirror device 70. The light is distributed through the optical fiber half 1 and guided to a photometry head 72 placed close to the measurement surface (back surface of the element) of the analysis slide 21 and irradiated thereon. This irradiation light is reflected from the measurement surface and transmitted to the light receiving element 74 through the optical fiber 73, and is then sent to the microcomputer.
etc., and the measured value is sent to the main body 1.
It can be displayed on the display window 76 of. This measured value may be printed on a roll of recording paper (not shown) if necessary.

前記測光ヘッド72は搬送路28内を走行する分析スラ
イド21の停止位置に複数個(図において4個)設けら
れ、各測光へノド72に連繋したそれぞれの光ファイバ
ー71の端面イ。
A plurality of photometry heads 72 (four in the figure) are provided at the stop position of the analysis slide 21 traveling in the conveyance path 28, and each photometry head is connected to the end face of each optical fiber 71 connected to the nodule 72.

口、ハ、二に回動ミラー装置70のミラー70aの角度
を設定することにより測光光線を誘導できるようにして
いる(第11図参照)、また測光ヘッド72は測光光線
の照射時には上動して第12図示のように分析スライド
21の測定面に圧着するようになっている。
By setting the angle of the mirror 70a of the rotating mirror device 70, the photometric beam can be guided (see Fig. 11), and the photometric head 72 moves upward when the photometric beam is irradiated. As shown in FIG. 12, it is pressed against the measurement surface of the analysis slide 21.

なお、ここに測光ヘッド72を複数設けたのは分析スラ
イドに血清を分注してから測光時までの時間差による複
数の測定を可能にしたレート分析法のためのものである
The reason why a plurality of photometry heads 72 are provided here is for a rate analysis method that enables a plurality of measurements based on the time difference between dispensing serum onto an analysis slide and photometry.

77は測光光線の光量等が経時的に変動することによる
測定値の誤差を可能な限りなくすために、測光光線の光
路に設置した45°に傾斜した透明ガラスで、該透明ガ
ラス77を反射する一部の光は受光素子78を介して補
正回路(図示せず)にリファレンスして分析スライド2
1の測定面から反射した測光光線の測定値を正しい値に
補正できる如くしている。
Reference numeral 77 is a transparent glass inclined at 45° installed in the optical path of the photometric light beam in order to eliminate as much as possible errors in measurement values due to changes in the light intensity of the photometric light beam over time. A part of the light is referenced to a correction circuit (not shown) through the light receiving element 78 and is sent to the analysis slide 2.
The measurement value of the photometric light beam reflected from the measurement surface 1 can be corrected to a correct value.

なお2図中79は測光用光学手段66にて測定後の分析
スライドの収納容器、80は分析項目等をインプットし
、各装置をスタートさせるための操作盤である。
In Figure 2, 79 is a storage container for the analysis slides measured by the photometric optical means 66, and 80 is an operation panel for inputting analysis items and the like and starting each device.

次に作用について説明する。Next, the effect will be explained.

まず1本体1の上面板1aに設けた円孔11を通してそ
の直下に停止しているディスク6に全血が適量入ってい
る試験管8を装着する0次いで操作盤80を操作して分
析項目等をインプットし、スタートさせる。これにより
駆動部3が作動してその出力軸4が3分の1回転して停
止するから、A位置のディスクはB位置へ、B位置のデ
ィスクはC位置へ2.C位置のディスクはA位置へ同時
に移動する。試験管8が装着されたディスク6がB位置
に停止すると、該位置に設置した回転付勢手段12が作
動する。即ちソレノイド13により可動板14が上動し
、摩擦部材17.18を介してモータ15とディスク6
の支軸6′が直結し、ディスク6を高速にて回転させる
。このディスク6の高速回転による遠心力で試験管内、
の全血はその比重差により血球と血清とが分離金れる。
First, insert a test tube 8 containing an appropriate amount of whole blood into the disk 6 that is stopped directly below it through the circular hole 11 provided in the top plate 1a of the main body 1.Next, operate the operation panel 80 to determine the analysis items. Input and start. This causes the drive unit 3 to operate and its output shaft 4 to rotate one-third of the way and then stop, so the disk at position A moves to position B, and the disk at position B moves to position C2. The disks at position C move to position A at the same time. When the disk 6 with the test tube 8 attached thereto stops at position B, the rotation urging means 12 installed at that position is activated. That is, the movable plate 14 is moved upward by the solenoid 13, and the motor 15 and the disk 6 are connected via the friction members 17 and 18.
A supporting shaft 6' is directly connected to rotate the disk 6 at high speed. Due to the centrifugal force caused by the high speed rotation of this disk 6,
Whole blood is separated into blood cells and serum based on the difference in specific gravity.

この分離に必要な時間の経過後2回転付勢手段12はデ
ィスク6′より離反する。ディスク6は図示しない制動
手段により制動され静止する。当該B位置における遠心
分離中にA位置では試験管8の装着作業が行われる。し
かる後、再び駆動部3が作動してその出力軸4が3分の
1回転し、B位置で遠心分離を終了したディスクはC位
置へ移動し。
After the time required for this separation has elapsed, the two-turn biasing means 12 separates from the disk 6'. The disk 6 is braked by a braking means (not shown) and becomes stationary. During the centrifugation at the B position, the test tube 8 is attached to the A position. Thereafter, the drive section 3 is activated again, and its output shaft 4 rotates one-third of a rotation, and the disk, which has finished centrifuging at position B, moves to position C.

先にA位置にて試験管を装着したディスクはB位置に至
り、上記同様に遠心分離される。
The disk to which the test tube was previously attached at position A reaches position B, where it is centrifuged in the same manner as above.

C位置へ移動したディスク6は試験管の一つがピペット
装入孔19に合致するように停止する2次いで、ピペッ
ト装置54のピペット主体58がピペット装入孔19を
通して下降し、先端が試験管内の血清層に装入し、吸引
機64の作動で適量の血清を吸引する。このC位置では
ディスク6が自転し、ピペット主体58にて吸引される
血清が選択されることは前述の通りである。
The disk 6, which has moved to position C, stops so that one of the test tubes is aligned with the pipette insertion hole 19.2 Next, the pipette main body 58 of the pipette device 54 is lowered through the pipette insertion hole 19, and the tip is placed in the test tube. The serum layer is charged, and an appropriate amount of serum is aspirated by operating the suction device 64. As described above, in this C position, the disk 6 rotates and the serum to be aspirated by the pipette main body 58 is selected.

吸引後ピペット主体5Bはピニオン60の作動により上
動し、モータ61により回動して分注孔53の直上に至
って再び下降し、血清を滴下する。一方、搬送路28に
は分析スライド21がその供給部38のプッシャー43
にて選択的に供給され1間歇搬送手段30の作動により
分注孔53に対応して位置しているから、前述のように
ピペット主体より滴下された血清は分析スライド21の
素子面に正しく供給されることとなる。素子面に血清が
分注された後の分析スライド21は前進し、測光用光学
手段66の測光ヘッド72の設置位置に至り、測光され
る。
After suction, the pipette main body 5B is moved upward by the operation of the pinion 60, rotated by the motor 61, reaches directly above the dispensing hole 53, and descends again to drip the serum. On the other hand, the analysis slide 21 is placed in the transport path 28 by the pusher 43 of the supply section 38.
Since the serum is selectively supplied by the pipette and positioned corresponding to the dispensing hole 53 by the operation of the intermittent conveying means 30, the serum dropped from the pipette main body as described above is correctly supplied to the element surface of the analysis slide 21. It will be done. After the serum has been dispensed onto the element surface, the analysis slide 21 moves forward and reaches the installation position of the photometry head 72 of the photometry optical means 66, where it is photometered.

この測光は測光ヘッドが上動して分析スライドの測定面
に密着後、光源67からの測光光線が測定面に照射され
、その反射光を受光素子を介してマイクロコンピュタ−
等の演算装置75により演算され、測定値を本体1の表
示窓76に表示する。同時に必要に応じてロール状記録
紙に記録される。
In this photometry, after the photometry head moves up and comes into close contact with the measurement surface of the analysis slide, the measurement surface is irradiated with a photometric light beam from the light source 67, and the reflected light is sent to the microcomputer via the light receiving element.
etc., and the measured values are displayed on the display window 76 of the main body 1. At the same time, the information is recorded on a roll of recording paper as required.

上述の操作は全自動にて行われる5 〔発明の効果〕 このように、この発明によれば、全血より血清を分離す
る遠心分離手段と2分析スライドを直線的に一定のピッ
チで搬送する手段を有し。
The above-mentioned operations are performed fully automatically.5 [Effects of the Invention] As described above, according to the present invention, the centrifugation means for separating serum from whole blood and the two analysis slides are transported linearly at a constant pitch. have the means.

かつ分析スライドの停止位置に複数個の測光ヘッドを有
するトンネル型の恒温槽と1分離血清を吸引し、恒温槽
内を通る分析スライドに分注できるピペット装置とを備
えたことを特徴としているから、被検サンプルとして全
血がそのまま利用でき、従来の如く別の工程で血清を作
る必要がな(被検サンプルの取扱が簡便であるばかりで
なく1分離後の血清をただちに被検サンプルとして使用
できる利点がある。
Moreover, it is characterized by being equipped with a tunnel-type constant temperature bath having a plurality of photometric heads at the stop position of the analysis slide, and a pipette device that can aspirate one separated serum and dispense it onto the analysis slide passing through the constant temperature bath. Whole blood can be used as a test sample, and there is no need to prepare serum in a separate process as in the past (not only is the test sample easy to handle, but the serum after one separation can be used immediately as a test sample). There are advantages that can be achieved.

また2分析者は全血を収容しん試験管を所定の如くセッ
トすれば、あとは全自動にて全血より血清の分離1分離
血清の吸引分注及び測定が一気に行われ9分析作業の能
率化が達成できるなど各種の優れた効果を奏するもので
ある。
In addition, once the analyst sets the test tube containing whole blood as specified, the entire blood serum is separated from the whole blood, and the aspiration, dispensing, and measurement of the separated serum are performed all at once, improving the efficiency of analysis work. It has various excellent effects such as the ability to achieve

【図面の簡単な説明】[Brief explanation of drawings]

図はこの発明の一実施例を示し、第1図は本体の外観斜
視図、第2図は遠心分離手段の平面図、第3図I、  
IIは高速回転前後の試験管保持容器の揺動状態を示す
断面図、第4図は遠心分離手段の正面断面図、第5図は
搬送路の断面斜視図、第6図I、  IIは分析スライ
ドの押圧部材の作動を示す図、第7図は押圧部材の起立
・前進時の説明図、第8図は分析スライドの供給部及び
測光用光学手段を示す概略図、第9図はプッシャーの斜
視図、第1θ図はピペット装置の斜視図、第11図は回
転ミラー装置の拡大図。 第12図は照射ヘッドと分析スライドとの関係を示す断
面図である。 1一本体      2−遠心分離手段6−ディスク 
   12一回転付勢手段2〇−恒温槽     21
−分析スライド28・−搬送路     3〇−間歇搬
送手段38′−供給部     43−・−プッシャー
54− ピペット装置  66−測光用光学手段特許出
願人     小西六写真工業株式会社第1図 第2図 第4図  。 第8図 第10図
The figures show one embodiment of the present invention, in which Fig. 1 is an external perspective view of the main body, Fig. 2 is a plan view of the centrifugal separation means, Fig. 3 I,
II is a sectional view showing the rocking state of the test tube holding container before and after high-speed rotation, FIG. 4 is a front sectional view of the centrifugal separation means, FIG. 5 is a cross-sectional perspective view of the conveyance path, and FIGS. 6 I and II are analysis. A diagram showing the operation of the pressing member of the slide, FIG. 7 is an explanatory diagram when the pressing member is raised and moved forward, FIG. 8 is a schematic diagram showing the supply section of the analysis slide and the optical means for photometry, and FIG. FIG. 11 is a perspective view of the pipette device, and FIG. 11 is an enlarged view of the rotating mirror device. FIG. 12 is a sectional view showing the relationship between the irradiation head and the analysis slide. 1 - Main body 2 - Centrifugal separation means 6 - Disc
12 One rotation biasing means 2〇-Thermostatic chamber 21
- Analysis slide 28 - Transport path 30 - Intermittent transport means 38' - Supply section 43 - Pusher 54 - Pipette device 66 - Optical means for photometry Patent applicant Konishiroku Photo Industry Co., Ltd. Figure 1 Figure 2 Figure 4. Figure 8 Figure 10

Claims (1)

【特許請求の範囲】[Claims] 全血より血清を分離する遠心分離手段と、分析スライド
を直線的に一定のピッチで搬送する手段を有し、かつ分
析スライドの停止位置に複数個の測光ヘッドを有するト
ンネル型の恒温槽と、分離血清を吸引し、恒温槽内を通
る分析スライドに分注できるピペット装置とを備えたこ
とを特徴とする生化学分析装置。
a tunnel-shaped thermostat having centrifugation means for separating serum from whole blood, means for transporting an analysis slide linearly at a constant pitch, and a plurality of photometric heads at the stop position of the analysis slide; A biochemical analyzer characterized by comprising a pipette device capable of aspirating separated serum and dispensing it onto an analysis slide passing through a thermostatic bath.
JP13399584A 1984-06-28 1984-06-28 Biochemical analyser Granted JPS6113158A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP13399584A JPS6113158A (en) 1984-06-28 1984-06-28 Biochemical analyser

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP13399584A JPS6113158A (en) 1984-06-28 1984-06-28 Biochemical analyser

Publications (2)

Publication Number Publication Date
JPS6113158A true JPS6113158A (en) 1986-01-21
JPH0439624B2 JPH0439624B2 (en) 1992-06-30

Family

ID=15117924

Family Applications (1)

Application Number Title Priority Date Filing Date
JP13399584A Granted JPS6113158A (en) 1984-06-28 1984-06-28 Biochemical analyser

Country Status (1)

Country Link
JP (1) JPS6113158A (en)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61247971A (en) * 1985-04-26 1986-11-05 Nippon Tectron Co Ltd Automatic analyzing device
JPS62276439A (en) * 1986-02-07 1987-12-01 Fuji Photo Film Co Ltd Chemical analyzer
JPS63229371A (en) * 1987-03-19 1988-09-26 Nittec Co Ltd Automatic analyzer
JPH01189562A (en) * 1988-01-25 1989-07-28 Nittec Co Ltd Transferring device of blood-collecting tube
JPH0287070A (en) * 1988-09-26 1990-03-27 Toshiba Corp Automatic apparatus for chemical analysis
US7150858B2 (en) 2000-08-18 2006-12-19 Arkray, Inc. Centrifugal separator
WO2009093731A1 (en) * 2008-01-25 2009-07-30 Arkray, Inc. Centrifuge, analysis device using the same, and vessel for centrifuge
WO2009101587A1 (en) * 2008-02-13 2009-08-20 Inpeco Ip Ltd. Centrifugation apparatus for containers of biological material
CN104198633A (en) * 2014-09-01 2014-12-10 周德波 Dual-circuit automatic fraction collection positioning equipment and positioning method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4960293A (en) * 1972-10-08 1974-06-11
JPS54113383A (en) * 1978-02-23 1979-09-04 Shimadzu Corp Method and apparatus for measuring enzyme
JPS5821567A (en) * 1981-07-31 1983-02-08 Fuji Photo Film Co Ltd Incubator

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4960293A (en) * 1972-10-08 1974-06-11
JPS54113383A (en) * 1978-02-23 1979-09-04 Shimadzu Corp Method and apparatus for measuring enzyme
JPS5821567A (en) * 1981-07-31 1983-02-08 Fuji Photo Film Co Ltd Incubator

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61247971A (en) * 1985-04-26 1986-11-05 Nippon Tectron Co Ltd Automatic analyzing device
JPH0380263B2 (en) * 1985-04-26 1991-12-24 Nippon Tectron Kk
JPS62276439A (en) * 1986-02-07 1987-12-01 Fuji Photo Film Co Ltd Chemical analyzer
JPS63229371A (en) * 1987-03-19 1988-09-26 Nittec Co Ltd Automatic analyzer
JPH01189562A (en) * 1988-01-25 1989-07-28 Nittec Co Ltd Transferring device of blood-collecting tube
JPH0287070A (en) * 1988-09-26 1990-03-27 Toshiba Corp Automatic apparatus for chemical analysis
US7150858B2 (en) 2000-08-18 2006-12-19 Arkray, Inc. Centrifugal separator
WO2009093731A1 (en) * 2008-01-25 2009-07-30 Arkray, Inc. Centrifuge, analysis device using the same, and vessel for centrifuge
WO2009101587A1 (en) * 2008-02-13 2009-08-20 Inpeco Ip Ltd. Centrifugation apparatus for containers of biological material
US8795144B2 (en) 2008-02-13 2014-08-05 Inpeco Holding Ltd. Centrifugation apparatus for containers of biological material including conveyor, turntable and centrifuges
CN104198633A (en) * 2014-09-01 2014-12-10 周德波 Dual-circuit automatic fraction collection positioning equipment and positioning method thereof
CN104198633B (en) * 2014-09-01 2017-06-30 周德波 The location equipment and its localization method of the automatic fraction collection of double circuit

Also Published As

Publication number Publication date
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