JPS6090096A - Preparation of deoxidized water - Google Patents

Abstract

PURPOSE:To lower the dissolved oxygen content in water efficiency, by bringing water to be deoxidized into contact with an immobilized microorganism obtained by immobilizing an aerobic or facultative anaerobic bacteria so as not to inhibit of its respiration action. CONSTITUTION:An aerobic or facultative anaerobic microorganism is immobilized in such a mode that its respiration action is not inhibited to prepare an immobilized microorganism. A water not substantially containing a nutritive substance or substrate to said microorganism is brought into contact with said immobilized microorganism to lower the dissolved oxygen content in water by the respiration action of the microorganism (1). Furthermore, it is also pref. that the aforementioned process (1) and a process (2) for bringing the immobilized microorganism into contact with water containing a nutritive substance to said microorganism, at the point of time the respiration action thereof is lowered to a predetermined level, to restore its respiration action are respectively performed at least one time.

Description

[Detailed Description of the Invention] Background of the Invention Deoxygenated water from which existing oxygen has been removed is commonly used as diluted water of highly concentrated fermented liquor obtained by various fermentations such as alcoholic fermentation, or as refreshing water.
Dilution water when producing fruit juice drinks, etc.

Alternatively, it has a wide range of uses, such as raw water and miscellaneous water (washing water, Zeiler water, etc.) in the food industry.

The vacuum method (Tecb) is a method for producing such deoxygenated water.
, Quart, Master Brew, As5
- Amer,, l 8.150°1980),
Heated carbon dioxide gas blowing method (Japanese Patent Application Laid-Open No. 57-201583, carbon dioxide gas blowing method (Tcch).

Quart, Master Brew, Ass.
tuner-, 18,136.

1981), 14 gas blowing method (Eur, Bre
w, Conv.

Proc, Congr, 17th, Berli
n (West), 245, 1979), etc. are well known. However, each of these methods requires equipment such as a military equipment, a heating device, a carbon dioxide gas blowing device, a nitrogen gas blowing device, a water deionization device, a p-filtration device, etc. The disadvantage was that the preparation cost and running cost were high.

The use of microorganisms such as yeast in a constant manner has already been carried out in many fields (in the food industry, medical products industry, and medical engineering). However, until now, identified microorganisms have only been utilized in the form of producing or degrading some kind of substance using the metabolic pathway of the microorganism.

Summary of the Invention The present inventors have also conducted research with the aim of producing useful substances using immobilized microorganisms, but in the process, it was discovered that immobilized yeast could continue to perform respiration activities for a long period of time even in water that did not contain any nutrients. To continuously and efficiently remove residual oxygen, and to regenerate microorganisms whose respiratory activity has decreased by providing nutrients.

They discovered this and arrived at the present invention.

Therefore, the method for producing deoxygenated water according to the present invention involves immobilizing aerobic or facultative anaerobic microorganisms in a manner that does not inhibit their respiration. and this water is substantially free of nutrients or substrates for the microorganisms) to reduce the longevity of the water as a result of the respiration of the microorganisms. It is something to do.

Another uninvented method for producing deoxygenated water is characterized in that steps 8 (1) and (2) below are each carried out at least once.

(1) Water to be deoxygenated to the immobilized microorganism's proboscis, which consists of an aerobic or facultatively anaerobic microorganism immobilized in a manner that does not inhibit its respiration (this water is (substantially does not contain any nutrients or -Mi).

(2) reducing the dissolved waste in the water as a result of the respiration of the microorganism; (2) when the respiration of the microorganism has decreased to a predetermined level, the water containing nutrients for the immobilized microorganism; The process of restoring its respiratory action by bringing it into contact with.

Effect 4' According to the invention, the immobilized microorganisms continue their respiration activity even in substantially nutrient-free water to lower the solubility of this water and that it can be used as a means of oxygenation of the water; and that this immobilized microorganism is regenerated by contacting it with nutrient-containing water when its respiratory activity decreases, and that it can be used as a regeneration process;
These findings were not only unexpected in themselves, but also unexpected considering the conventional usage of immobilized microorganisms as described above. Good morning.

According to the present invention, the bath oxygen concentration is o - o, t ppm
8 summer deoxygenated water can be easily produced.

The deoxygenated water thus obtained is used for microorganisms.

For example, a slight increase in TOC (total organic carbon) produced by yeast (usually 2 to 5 ppm) and the addition of a slight yeast odor are observed, but these can be treated with sterilizers, sterilizing filters, active agents, etc. Since it can be easily and completely removed by the method, there is no particular problem in practical use. Therefore, according to the present invention, it can be said that the problems of the conventional deoxygenated water apparatus, which uses a large-scale, high-cost equipment, and the like are solved.

3. Detailed Description of the Invention Deoxygenation The deoxygenation step according to the invention consists of contacting the water to be deoxygenated with immobilized microorganisms.

Immobilized microorganisms The immobilized microorganisms used in the present invention consist of microorganisms that are aerobic or anaerobic and are supported by a sail that does not impede their respiration. .

These five types of hardened proboscis are well known, and first of all, there are microorganisms such as yeast, mold, and microorganisms.
Examples of solid carriers include alginate/calcium acid, mono-crosslinked carrageenan, Manonano, and Aigyokun.

Such immobilized microorganisms have recently attracted particular attention as new bioreactors, and their specific contents, such as the tub of microorganisms to be immobilized,
, the immobilization method, the shape of the immobilized product, etc. are written in the tax, theory, and command of each building (for example, ``fermentation and industry'',
[Refer to Volume 35, Nos. 1 to 5] In the present invention, conventionally known immobilized microorganisms can be used as appropriate by simply taking into consideration that the proboscis to be immobilized is aerobic or facultatively anaerobic. be able to. In the present invention, "1-microorganisms are immobilized with mQ that does not inhibit their respiration" means essentially the same state as the mode of immobilization in order to utilize the biological activity of immobilized microorganisms in a Nononio reactor. means.

A preferred specific example of the immobilized microorganism used in the present invention is one in which the microorganism is yeast. Examples of the immobilization carrier include alginic yeast calcium/aluminium. Regarding immobilized yeast, in addition to the above-mentioned references, J. In5t, Brew,
Volume 84 s m 22B-230 (1978),
Enzyme Mitrobe, Technol, 5th
vol., pp. 41-45 (1983), and Brauw.
issenschaft-Volume 35.

254-258 (1982) et al.

Yeast that should be used as such immobilized yeast include:
Saccbaromyces uvarum, S
, cerevisiae and the like. It is desirable to select a strain that continues to actively respire for a long time even in water with no nutrients.

This is because the microorganisms used for deoxygenation are used to consume dissolved oxygen in water that is virtually devoid of nutrients. In order for microorganisms to survive for a long period of time in a starved state, it is desirable that the microorganisms have a sufficient amount of glycogen, such as glycogen, in the cells immediately after being cultured. In addition, in order to alleviate the starvation state, the okiya-ized carrier may contain a nutritional component for the microorganism,
Alternatively, a small amount of a fertiliser, which is beneficial to the microorganisms, can be added to the water to be deoxidized (details will be described later).

Since the deoxidized water should be used for the above-mentioned purposes, the present invention is based on the use of vI quality as a substitute for immobilized microorganisms. Therefore, the water to be deoxidized, which is the object of the present invention, is substantially free of nutrients or substrates for the organisms that use it.
Ru. Here, "substantially free of" means that substantially no nutrients or substrates or their metabolites are present in the water from the area where silicon has been removed. As mentioned above, small amounts of nutrients may be added to the treated water in order to alleviate excessive starvation of the microorganisms used and to prolong their survival.

The water to be deoxidized that is the object of the present invention is typically tap water, well water, groundwater, or the like.

Deoxidation Step The deoxidation step can be carried out in a manner essentially not different from conventionally known techniques in which immobilized microorganisms are used as a bioreactor. Therefore, immobilized microorganisms in the form of granules, threads, glands, etc. may be brought into contact with the water to be treated in a fixed bed, moving bed, or fluidized bed. Specifically, if one or several deoxidizing columns made of granular immobilized microorganisms are packed in a cylindrical container are connected in rows and/or in parallel, the water to be treated can be passed through. good.

The temperature in the deoxidation step is preferably within a range that allows the microorganisms used to maintain their respiration for a period of time at an appropriate respiration rate. The contact time between the immobilized microorganisms and the water to be treated must be sufficient to obtain the desired deoxidizing effect. This time can be easily determined by a simple experiment depending on the given microorganism species, the oxygen concentration of the water to be treated, the degree of oxygen removal process, etc.

After the deoxygenation treatment, heating or application of reduced pressure to the reservoir for removing carbon dioxide produced as a result of respiration, and other post-treatments may be performed as necessary.

(14 days) Since the deoxygenation of the present invention is carried out in a starved state of the microorganism used, the respiratory activity of the microorganism decreases as the stored nutrients in the proboscis of the microorganism used are consumed.

When the respiratory activity of the microorganism used has decreased to a certain level,
For example, Final Survival! At the point when the elemental concentration does not fall below 0.1 ppm, add nutrients to the microorganisms, such as wort, etc., to the water to be treated if the microorganisms are yeast. When this happens, the microorganisms absorb nutrients and accumulate glycogeno within their bodies.

Restores respiratory activity.

Such a regeneration step is usually carried out separately from the deionization step, ie both steps are carried out alternately, at least once each. In addition, if possible, instead of performing both steps separately, a small amount of nutrients may be added during the remission step to prevent the regeneration step (or to prevent a decrease in respiratory activity).
However, as mentioned in 6c above, it may be implemented at the same time.

Experimental Example Example 1 Volume tit 1800cm3 (φ7cm x 47cm)
A cylindrical column with 1% algium/calcium acid added at a loading rate of 30
Brewer's yeast (S accbc: ro + n
yces uvarum) was formed into beads with a diameter of 3 mm and filled with a filling rate of 80%.

1200-2200cm3 per hour at 2℃
(D city water (remaining oxygen 14 ppm) flow rate 31.2 ~ 57
．． The flow rate was 20m/hr. From the outlet of the cylindrical column, deoxygenated water with a residual oxygen level of 0 to Q and 1 pprn was stably obtained over India and Japan.

The method for identifying yeast on Algi/L&calcium gel is as follows. First, 301 brewer's yeast is added to 101n1 of an aqueous solution of l-thialginic acid as a precursor to obtain a yeast suspension. This is 0.1 M
The mixture is dropped into an aqueous solution of Lucicum chloride to obtain a piece-shaped immobilized yeast with a diameter of 3 cm.

Example 2 In a cylindrical column with a volume of 11500 cm (φ5cxn
es uvarum ) tx 5 mm square cubes were filled at a filling rate of ω%. At 2[deg.] C., 250-400 cm30 city water (11 pp+n dissolved in water) was added to the reactor per hour. From the cylindrical column outlet? Culm existing oxygen degree θ ~ 0.3 ppm.

De-L/ν talk water was obtained consistently for more than 60 days.

The method for identifying yeast in Aigyokushi gel is as shown in the arrow. First, add brewer's yeast log to 100ml of water)
Let it remain still, add 7 Aigyoku achenes [Og] and heat it.
After stirring for a minute, immediately squeeze with gauze to remove solids. Next, the obtained sol is stretched into a plate shape, and 0.05 M Ca1l water me is added thereto and left to stand for 2 hours to gel. This gel is cut into 5 mm square dice and further immersed in a 0-05 M Ca C12 water G solution for one day and night to obtain identified yeast.

Applicant's agent Kiyoshi Inomata

Claims (1)

[Scope of Claims] 1. Water to be deoxygenated to immobilized microorganisms consisting of aerobic or facultatively anaerobic microorganisms immobilized in a manner that does not inhibit their respiration (this water, however, The method is characterized in that the amount of oxygen present in the water is reduced as a result of the respiration of the microorganisms by absorbing the water (substantially free of nutrients or substrates for the microorganisms). Method for producing deoxygenated water. 2. The microorganism is yeast. 1. The method according to claim 1. 3. A method for producing deoxygenated water, characterized in that q:J' comprises carrying out each of the following steps (1) and (2) at least once. il+ Aerobic or facultative η11 [Water to be deoxidized by immobilized microorganisms, consisting of aerobic microorganisms immobilized in a manner that does not inhibit their respiration (this water does not contain nutrients or substrates for the microorganisms) (21) A step in which the amount of iron present in the water is reduced as a result of the respiration of the microorganism (21) when the respiration of the microorganism has decreased to a predetermined level. 4. The method according to claim 3, wherein the immobilized microorganism is brought into contact with water containing nutrients for the microorganism to restore its respiration. 4. *The method according to claim 3, wherein the organism is yeast.