JPS6073343A - Spectrophotometer - Google Patents

Abstract

PURPOSE:To enable high speed and high accuracy analysis by eliminating the change-over of a filter, by guiding white light to a plurality of specimen cells by using a plurality of optical fibers to which said cells respectively correspond, and simultaneously subjecting transmitted luminous flux to spectral diffraction by a concave diffraction grating. CONSTITUTION:The light of a light source lamp 1 is condensed by a condensing lens 2 and incident to a plurality of optical fibers 4. The lights from the optical fibers are incident to respectively corresponding measuring cells 6 while lights transmitted through a specimen solution are incident to an optical fiber 5 to form a transmitted luminous flux line. This luminous flux line is condensed by a condensing lens 12 and passed through an incident slit 13 to form spectra dispersed at every luminous flux by a concave diffraction grating 15 and the spectra are taken out as electric signals by the line of light detectors 17. Therefore, mechanical driving such as the change-over of a filter is not necessary at all and high speed and high accuracy analysis can be performed.

Description

[Detailed Description of the Invention] B] Industrial Application Field The present invention is directed to a spectrophotometer that can measure a small number of analysis items simultaneously. This relates to the spectrophotometric intensity t1 of the automatic analysis WAN that can increase the analysis of analysis items by 1j at +1jj.

(b) The spectrophotometric intensity n1 from the prior art 1 is 1fflffft so as to measure the absorbance of the sample accommodated in - number of measurement cells,
Because it is manufactured, for example, conventional spectroscopic r! ! ! ! When incorporating 1 and performing analysis on a large number of analysis samples or analysis items, the photometry operation may be repeated for each analysis sample or analysis item, or the photometry operation may be performed automatically. It is necessary to adopt a method that requires a large number of mBt of analytical light corresponding to the number of analysis samples or analysis items that can be processed at one time by the analyzer, and therefore a large amount of FR space is required. However, since the automatic analysis equipment has to be complex and huge in scale,
There were problems in terms of time, location, and accuracy.

As a way to solve this problem, a formula was developed that calculates the absorbance of multiple measurements by passing different monochromatic lights through the same measurement cell. Therefore, it is essential to switch the filter, and therefore a mechanical drive for rotating the filter or rotating the mirror is required, which makes the structure complicated, and
There were problems such as a decrease in the measured V4 degree due to vibrations caused by the mechanical drive.

This 1]! ! In addition, a method was developed in which C-neutralized light was passed through a photometer using a diffraction grating. When analyzing by direct photometry, Ej,
It was difficult to compensate for the decrease in accuracy due to buffing of the structure of the reaction tube/measuring tube or variations in the optical path, which was a problem.

(C) Purpose The spectrophotometric intensity 31 of this light is obtained by passing white light through a plurality of sample rails and then making each of the transmitted lights incident on different positions of the same concave diffraction grating. It spectrally spectrally transmits the total -C light and determines (1), and it provides a spectrophotometer that solves all the problems faced by conventional spectrophotometers at once.

In particular, the spectrophotometer of the present invention is not bulky even when incorporated into an automatic analyzer, and can be used for a large number of analytical samples and a large number of analytical devices while maintaining high analytical accuracy. ]
Spectrophotometry that allows you to conduct analysis in an extremely short time! ! The purpose is to provide 1-.

(d) Configuration The present invention provides a plurality of optical fibers, each of which has its optical input end disposed in a transmission optical path from a corresponding photometric rail, and whose optical output end connects to a plurality of optical fibers arranged in a row. A slit having a gap formed to match the light flux row emitted from the light output end of the row, and a spectral surface matching the light flux power from the slit are 'Or'.
The present invention is a spectrophotometer characterized by comprising a spectroscopic element J' and photodetectors arranged in two-dimensional directions such as length and breadth.

In the spectrophotometry B1 of the present invention, the optical fiber used may be any type of optical fiber, including optical fibers made of glass, optical fibers made of synthetic resin, and 1f, regardless of whether or not they are coated, as long as they can transmit the C1 light. It can also be used to draw.

The number of optical fibers used corresponds to the number of +1 photometric cells, and corresponds to the number of rows of photodetectors installed on the light detection surface.The optical input and output ends of each optical fiber are , Directly or blizzard on the transparent part of the compatible photometer
3 optically indirectly through the optical element of X6! ! Touch.

It is preferable that the optical output end of the optical fiber is fixed 8 to the optical fiber holder by appropriate means so that the transmitted light beams transmitted from the output end are lined up in a line H at regular intervals.

In addition, in the spectrophotometer n1 of the present invention, the slit can be used as an optical slit in the optical C1 device. It is necessary that each luminous flux component of the flux array be sufficiently clear to pass through.

At the light output end of the optical fiber and the slit, optical elements such as a condenser lens, a mirror, and a collar may be appropriately provided. If only the condenser lens is 1'JIJ, the optical output end of the optical fiber 7 is arranged in the 13 direction and the slit is 17! It is preferable that the longitudinal direction of the I-gap can be made to coincide with each other because it prevents light diffusion and simplifies the structure. In that case, if the optical output ends of the optical fibers are stacked vertically and arranged, the longitudinal direction of the slit's ll!I gap is @
If the ends of the dispensing horns are arranged horizontally, the longitudinal direction of the gap between the sulins 1- will be formed horizontally.

In the spectrophotometer 81 of the present invention, (b) the optical element has the function of splitting light into its components, for example,
Spectroscopic elements such as prisms, plane diffraction gratings, concave diffraction gratings, transmission type diffraction gratings, etc. can be used with appropriate arrangement methods and optical systems. However, concave diffraction gratings are of the reflective type, It is preferable to use a concave diffraction grating as a diffraction grating because it saves money on external dimensions up to 1000 ml of light when reflecting light, and it is also easy to manufacture and inexpensive. When splitting light using a prism, the dispersion plane of the dispersion element is arranged so that each beam of the transmitted beam array from the slit is equally dispersed.Therefore, when using a concave diffraction grating, the multiple The direction of the diffraction groove is aligned with the direction of the array of transmitted light beams from the slit.

Optical elements such as lenses and mirrors can be installed between the slit and the spectroscopic element, but unless the optical path is changed using a mirror, the longitudinal direction of the gap between the slits and the direction of the diffraction member of a concave diffraction grating, for example, can be It must be done.

In the spectrophotometer of the present invention, multiple v1 analysis samples r1
In order to perform spectroscopic analysis simultaneously for a plurality of analytical items or for a plurality of analysis items, a photodetector surface with photodetectors arranged in two directions of the masonry, for example, is used. The direction in which the photodetectors are arranged is determined depending on, for example, the direction in which the light output ends of the optical fibers 5 are arranged or the direction of the light beam array passing through the slit. In any case, the spectrum (q) is developed in the direction perpendicular to the direction in which the diffraction grooves of the diffraction grating extend. Accordingly, the rows of photodetectors correspond to the number of optical fibers that can be used at least 1 r, that is, the number of optical fibers 5. The photodetectors are arranged in rows so that any wavelength can be selected from the available spectrum at any time depending on the analysis item. Alternatively, a photodetector can be placed only at a certain wavelength position.

(e) Example diagram is an explanatory diagram showing an example of the spectrophotometer of the present invention.

A condensing lens is provided in front of the light from the light source lamp 1, and an optical fiber holder 3 is provided at the condensing position of the condensing lens 2 to connect the light input end of each of the plurality of optical fibers 4 to the light source 1. Fix it to the side. Each optical fiber 4 is guided to a corresponding photometric transmission section 9. In the transmission section 9 of the photometric cell 6, prisms 7 and 8 are arranged on both sides of the photometric cell, and each prism is connected to an optical fiber 4.
5 are optically connected. Therefore, the light from the optical fiber 4 passes through the prism 7, the photometric cell, and the prism 8, and then enters the optical fiber 5.

The tip ends of the optical fibers 5 are stacked vertically and fixed to the optical fiber holder 10. A collimator lens 11 is fixed to the optical fiber holder 10, and a condensing lens 12 is arranged in front of the collimator lens 11. Incident Surin] −
13 is provided at a position where the light beam array from the condenser lens 12 is condensed. The gap 14 between the entrance slits 13 is formed in accordance with the arrangement direction of the light output ends of the optical fibers 5, that is, to extend in the vertical direction. A monochromator is provided in front of the entrance slit. The monochromator is provided with a concave diffraction grating 15 and a light detection surface 18. Since the concave diffraction grating is reflective, the spectrometer can be made more compact. The diffraction grooves 16 of the concave diffraction grating 15 are located between the entrance slits 13 and I! It is formed in accordance with the longitudinal direction of 1l114. On the light detection surface 1, the photodetectors 17 are arranged in a circular manner on the base, vertically and horizontally, and the number of rows of photodetectors arranged in the vertical direction is equal to the number of optical fibers 5. It must be more than that. In this embodiment, the optical output ends of the optical fibers 5 are arranged one on top of the other, but the invention is not limited to this, and for example, they may be arranged side by side. In this case, the entrance slit 13
In the longitudinal direction of the gap 14, the diffraction groove 16 of the concave diffraction grating 15
The photodetectors 17 on the photodetecting surface 18 are arranged in the horizontal direction in accordance with the arrangement of the optical fibers 5.

The operation of the spectrophotometer of this embodiment during photometry will be described.

Light emitted from a light source lamp 1 is condensed by a condenser lens 2 and enters the light input end of an optical fiber 4 held by an optical fiber holder 3. The light incident on the optical fiber 4 is propagated by the optical fiber 4, enters the photometric cell transmission section 9, and enters the photometry cell 6 from the prism 7 in the photometry cell transmission section 9. The light that has passed through the sample solution in the photometric cell 6 enters the prism 8 . Photometric cell 6
The light that has passed through the sample solution inside enters the optical fiber 5 from the prism 8, is propagated along the optical fiber 5, and is emitted from the optical output end of the optical fiber 5. Since the output ends of the optical fibers 5 are stacked and fixed in the vertical direction,
The emitted transmitted light beams form a transmitted light beam array aligned in the vertical direction. The entrance slit 13 forms a gap in accordance with the arrangement of the light output ends of the optical fibers 5, so that the total (1ljl) of the transmitted light flux array can pass through the slit 13.

Once, the transmitted light beam array emitted from the optical fiber 5 is collected by the condenser lens 12, and the inlet side sulin 1-13 is collected.
The light passes through the gap, becomes a defined transmitted light beam train, and enters the concave diffraction grating 15 of a part of the monochromator. The transmitted light beam array incident on the diffraction grating 15 is dispersed by the vertically extending diffraction grooves 16 to form a spectrum.

The spectrum of each luminous flux is expressed horizontally from above or below in the order in which the light output ends of the optical fibers 5 are arranged on the light detection surface 18.
Each of the signals is detected as an electrical signal by a row of photodetectors 17 on a photodetecting surface 18.

The electrical signal is data-processed, for example, two wavelengths are taken out from the spectrum, the difference is calculated, and the activity value or concentration value for each analysis sample or analysis item is estimated from the detection ffi line. Then, output the data from the output device.

(F) Effect The spectrophotometric S1 of the present invention uses an optical fiber to form a plurality of transmitted lights that have passed through a plurality of photometric cells into one beam train, and simultaneously spectrally spectra them without disturbing this beam train. hand,
It forms corresponding spectra on a plane and measures the light at the same time, and does not require any mechanical drive such as switching filters as seen in conventional spectrophotometers of this type. Not only is the structure simple q1, but it is also possible to avoid a decrease in the measured RI due to ambient vibration or the like.

Moreover, the spectrophotometer of the present invention can simultaneously measure the absorbance of multiple sample solutions stored in multiple photometric barrels at multiple monochromatic wavelengths.
We can analyze multiple items to the extent that conventional spectrophotometers cannot.
It can be carried out at high speed and with high accuracy, and can be applied to automatic analyzers to expand their functions, which has a great impact.

[Brief explanation of the drawing]

The figure is an explanatory diagram showing an embodiment of the spectrophotometer of the present invention, in which 1 is a light source lamp, 2.12 is a condensing lens, 3 is an optical fiber holder, 4.5 is an optical fiber, 6 is a photometric cell, 7.8 is a prism, 1° (1°) is an optical fiber holder that fixes the light output end of the optical fiber 5, 11 is a collimator lens, 13 is to the Surin 1, and 14 is the 11
1 gap, 15 is a concave diffraction grating, 16 is a diffraction groove, 17 is a photodetector, and 18 is a photodetection surface. Hand: 1st continuation amendment, correct position 1' 1, Incident display Sho+1I58 special; yrm m 182420 No. 2,
Name of the invention Spectrophotometer 3, Relationship to the case of the person making the amendment Special agreement Applicant name (199) Shimadzu Corporation Name (
75 (lid) Patent Attorney Takeshi 1) Masahiko 5, Date of amendment order; The phrase "optical fiber 5" in the 9th line and in the 17th to 18th tT lines of the same page is corrected to "optical fiber."

Claims (1)

[Claims] Each optical input end is disposed in a transmission optical path from the corresponding photometric cell, and the optical output end thereof is emitted from the plurality of optical fibers arranged in a column and the light output end of the column. The gap formed according to the light beam array is N! A spectrophotometric bar comprising a spectroscopic element having a spectroscopic surface that matches the beam array of the slit, and a photodetector arranged in a two-dimensional direction such as a structure. 1゜