JPS60252246A - Spectral imaging device for microscopic image - Google Patents

Abstract

PURPOSE:To analyze the fine structure of a material by irradiating a sample such as a vital cell by UV rays having high energy and observing the luminous image thereof. CONSTITUTION:The light from an excimer laser device 1 forming a UV light source is reflected by a reflecting mirror 2 and irradiates a sample 3. The sample 3 emits light such as fluorescence or phosphorescence when irradiated with the UV rays. The luminous image thereof is magnified by a microscopic optical system 4. An acoustooptic device 5 having an acoustooptic element 51 consisting of an acoustooptic crystal such as tellurium dioxide extracts the image of an optional wavelength component from the luminous image contg. various magnified optical components. The picture image information selected in the above-mentioned manner is picked up by a TV camera 7. The picked up image is processed by a picture image processing unit 8 and is displayed on a display device 9.

Description

[Detailed description of the invention] (Industrial application field) The present invention is a microscopic image (1) of a sample irradiated with an ultraviolet light source. The present invention relates to a spectroscopic imaging device.

(Prior Art) Many substances emit light such as fluorescence or phosphorescence when irradiated with high-energy light (ultraviolet light).

The light emission also has different wavelengths depending on the atomic structure and single molecule structure.

Therefore, by separating the luminescence image into each wave sphere and observing the images, it is possible to elucidate the fine structure of the material.

Therefore, there is a strong desire to observe luminescence images corresponding to ultraviolet excitation of living cells.

(Object of the Invention) An object of the present invention is to provide a spectroscopic imaging device for microscopic images of a sample irradiated with an ultraviolet light source.

(Structure of the Invention) In order to achieve the above object, the present invention provides a spectroscopic imaging device for microscopic images, which includes an ultraviolet light source, a sample that supports a sample excited by the ultraviolet light source, and a sample that supports the sample excited by the ultraviolet light source. It is composed of a microscope optical system (2) that magnifies an image, an acousto-optic device that extracts an image of an arbitrary wavelength component included in the expanded emission image, and an imaging device that captures an image of the arbitrary wavelength component. There is.

(Explanation of Examples) The present invention will be described in more detail below with reference to the drawings and the like.

FIG. 1 is a schematic diagram showing an embodiment of a spectroscopic imaging apparatus for microscopic images according to the present invention.

Purple (excimer forming a line light source (F, XIMEII)
The light from the laser device 1 is reflected by the reflecting mirror 2 and the light from the laser device 1 is reflected by the mirror 2. P, irradiate the sample 3 supported on one unit.

As an excimer laser device, xeF (wavelength 351 nm)
, XeCn (wavelength JK308nm), KrF (wavelength 2
48 nm), ArF (wavelength 193 nm).

F2 C waves (157 nm), etc. are known.

When sample 3 is irradiated with violet A rays, it emits light such as fluorescence and phosphorescence. This luminescence image is magnified by a microscope optical system.

Acousto-optic bag W from an enlarged luminescent image containing various light components
5 extracts an image of an arbitrary wavelength component (3) included in the emission image.

FIG. 2 is a schematic diagram for explaining the principle of generation of diffraction in an acousto-optic device.

When an acousto-optic element 51 is formed with the incident and exit planes of an electro-optic crystal such as tellurium dioxide (Te02) as an optical plane and is excited by a variable frequency oscillator 53 using an acoustic transducer 54, the incident light is Bragg diffracted. be able to. 55 is an acoustic 1g absorber.

The diffraction angle θd is given by the following formula.

θd−(λ/v)f where λ: Wavelength of light included in the incident light ■; Speed of sound f: Excitation frequency As shown in Fig. It is focused towards the plate 52.

If the excitation frequency is fl and light with wavelength λ1 coincides with the aperture of the aperture plate 52, only (4) image information possessed by the wave λ will be extracted from the light emitted from the sample. .

Similarly, when another frequency is selected by the variable frequency oscillator 53, the image information y of the leakage λ2 corresponding to the selected wave number G is obtained 111.

The image information selected in this manner is captured by the camera 7, processed by the image processing device 8, and displayed by the display device 9.

(Results of the Invention) As explained above, the spectroscopic imaging apparatus for microscopic images according to the present invention includes an ultraviolet light source, a sample supporting a sample excited by the ultraviolet light source, and an emission image of the 8υ It consists of a microscope optical system that magnifies the light, an acousto-optic device that extracts an arbitrary '/& long component component image contained in the enlarged luminescence image, and an imaging device that captures an image of the arbitrary wavelength component. .

Therefore, by irradiating a sample such as a living cell with high-energy ultraviolet rays, an image formed by the fluorescence or phosphorescence of the living cell can be extracted as an image for each wave of the light emission.

[Brief explanation of drawings]

(5) FIG. 1 is a schematic diagram showing an embodiment of a spectroscopic imaging apparatus for microscopic images according to the present invention. FIG. 2 is a schematic diagram for explaining the principle of generation of diffraction in an acousto-optic device. FIG. 3 is a schematic diagram for explaining the principle of spectroscopy of an acousto-optic device. DESCRIPTION OF SYMBOLS 1...Laser device 2...Reflector 3...Sample 4...Microscope optical system 5...Acousto-optic device 51...Acousto-optic crystal 52.
...Aperture plate 53...Variable frequency oscillator 54...Acoustic transducer 55...Acoustic absorber 56...Lens 7...Television camera 8...Arithmetic processing unit 9...Display device patent Applicant Hamamatsu Photonics Co., Ltd. Agent Patent Attorney Hisashi Inoro (6)

Claims (1)

[Claims] fll An ultraviolet light source, a sample stage that supports a sample excited by the ultraviolet light source, a microscope optical system that magnifies an emission image of the sample, and an arbitrary wavelength component included in the enlarged emission image. 1. A spectroscopic imaging device for microscopic images, comprising an acousto-optic device that captures an image of the wavelength component, and an imaging device that captures an image of the arbitrary wavelength component. (2) The V9 microscopic image spectroscopic imaging apparatus according to claim 1, wherein the ultraviolet light source is an excimer laser light source. (3) The acousto-optic element included in the acousto-optic device is installed.