JPS60248137A - Chewing gum composition containing mutastein incorporated therein - Google Patents

Abstract

PURPOSE:A chewing gum composition, obtained by incorporating a gum base raw material with a specific saccharide sweetening raw material, physiologically active substance mutastein and other additives, and containing the low carious mutastein capable of retaining the activity stably with time. CONSTITUTION:A chewing gum composition obtained by incorporating (A) a gum base raw material, e.g. chicle or ester gum, with (B) a saccaride sweetening raw material constituted of (i) sugar and (ii) a disaccharide other than the sugar, preferably (iso)maltose, palatinose or alpha (or beta)-lactose, (C) a physiologically active substance mutastein and (D) an additive raw material, e.g. mint based perfume or fruit based perfume. The amount of the component (B) to be incorporated is preferably 50-90wt% based on the total amount of the composition.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a chewing gum composition containing mutastein, and more specifically, it uses sugar and trisaccharides other than sugar as main sugar sweetening raw materials, and has a cariogenic effect. The present invention relates to a chewing gum composition with low caries properties, which contains mutastein, a physiologically active substance having the following properties.

[Prior art and its problems]

In recent years, the main cause of dental caries is that oral bacteria produce glucans from sugar, which adhere and accumulate on the teeth to form dental plaque, and the action of bacteria based on this leads to dental caries. Dextranase and mutanase have been developed as enzymes that decompose glucans, which are the main components of dental plaque, and have been used to prevent dental caries.

In the field of chewing gum, attempts have been made to develop chewing gums to prevent caries. For example, there have been reports of chewing gums in which the sugar that causes caries is replaced with other sugars or sweeteners, and chewing gum compositions containing the above-mentioned dextranase or mutanase. be.

However, a physiologically active substance, mutastein, which inhibits the production of glucans from sugar by Streptococcus mutans, an oral bacterium, has recently been discovered (5%).
7-/Ko 4117), the prevention of wax corrosion due to its non-conventional function has attracted attention.

[Problem that the invention seeks to solve]

Regarding the incorporation of the new physiologically active substance mutastein into chewing gum, its stability was a completely unknown field.

[Means for solving problems]

Therefore, the inventors of the present invention, including the discoverers of Mutastein, have conducted extensive trial research into incorporating Mutastein into chewing gum, and have found that when chewing gum uses conventional sugar and glucose as the main sugar sweetening ingredients, Mutastein However, if it is mainly used in combination with sugar and trisaccharides other than sugar, mutastein can be stably incorporated into chewing gum.
The present invention was completed by discovering that a chewing gum composition with low caries susceptibility can be obtained without impairing the flavor palatability of chewing gum.

[Purpose of the invention]

Therefore, a general object of the present invention is to provide a low-caries chewing composition, and a further object is to provide a low-caries chewing composition that contains the new physiologically active substance mutastein and maintains its activity stably over time. providing a chewing gum composition;
Still another object is to provide a low-cariogenic chewing gum composition containing mutastein that is equivalent to or better than conventional chewing gums that use sugar and are highly palatable.

[Key points of the invention]

In order to achieve the above-mentioned object, in the mutastein-containing chewing gum composition according to the present invention, the company, gum paste raw materials,
A chewing gum composition consisting of sugar sweetening raw materials and other chewing gum additive raw materials, in which the sugar sweetening raw materials are mainly composed of sugar and one or more trisaccharides other than sugar, and the physiologically active substance mutastein is blended. Mutastein used in the present invention is a new physiologically active substance with a molecular weight of 200,000 or more that has the properties of a protein and was discovered in the culture solution of Aspergillus terreus, a type of koji mold, and is a new physiologically active substance with a molecular weight of 200,000 or more, which is a cause of dental caries. Although it does not have an antibacterial effect on Streptococcus mutans, an oral bacterium that synthesizes glucans, which are the main components of plaque, instead of sugar, it does have the effect of specifically inhibiting the synthesis of glucans. The mutastein used in the present invention is not limited to that obtained from the culture solution of Aspergillus terreus, but any product of other Aspergillus or other bacteria that can be identified as mutastein can be used. Mutastein is blended at about 0.0/~0.06% with respect to the chewing gum composition, and the blending is controlled so that the temperature does not exceed 30°C when mixing the chewing gum, and the mixture is added and kneaded at the end to obtain the chewing gum composition. .

The trisaccharide other than sugar used in the present invention is a trisaccharide selected from the group consisting of maltose, isomaltose, palatinose, α-lactose, and β-lactose, and sugar and one or more of the above trisaccharides. The amount used in combination with the chewing gum composition! OP.

% by weight, preferably 60-0% by weight. The weight ratio of sugar and trisaccharides is 2j near 7~ri! :! So, preferably 1j
AO Nige O~IjE/! It is.

When this weight ratio is on the side with less sugar than Jj17j, the sweetness is lower and tends to suppress flavor diffusion.
The palatability of chewing gum decreases. On the other hand, Ri! ! ! When the amount of sugar is higher, the effect of suppressing the decline in mutastein's activity over time is weakened.

Maltose (malt sugar) and isomaltose.

There are cases where refined maltose and maltose and isomaltose derived from corn syrup are combined, and cases where corn syrup with a high content of maltose and inomaltose are used, but in both cases there is no problem if used in the specified ratio with sugar. There isn't. Maltose is 3 to 4 tons of sugar.
Since it has a sweetness on the order of O-, if too much is used, the sweetness decreases too much and the diffusion of flavor is suppressed.

What is the weight ratio of maltose and isomaltose? Q:! O~ri5:! used within the range.

Palatinose is also called inmaltulose, and when it is combined with sugar, maltose and isomaltose are included in the mixture, so the amount exceeds the specified amount range. Therefore, in this case it is necessary to use palatinose syrup. Since the sweetness of palatinose is about the same as that of sugar, it exhibits the same tendency of action as maltose. The weight ratio of sugar to palatinose is 30.

Furthermore, combinations of maltose, isomaltose and palatinose can also be suitably used, with weight ratios of sugar and mixed disaccharides such as maltose, isomaltose and palatinose being used in the range 2j to rrtt.

The gum paste raw materials used may be those commonly used, such as chicle and jelutong.

Vegetable resins such as Gatasiak, vinyl acetate resins, natural and synthetic rubbers, ester gums, waxes, emulsifiers, inorganic fillers, and the like can be formulated in generally known formulations.

Other chewing gum additive raw materials used are mainly flavorings, and depending on the purpose, for example, mint flavorings (peppermint oil, spearmint oil), fruit flavorings (citrus oil, banana, pine, strawberry), etc. -, etc.), spices, coffee-based fragrances, etc.

[Embodiments of the invention]

Next, the present invention will be specifically explained with reference to Examples.

Examples shown below/~! A chewing gum of Control Example/2.2 was prepared and tested.

In the table, the gum bases are of a known general formulation, and include natural chicle (JO parts), vinyl acetate resin (7 parts), polyisobutylene (30 parts), ester gum (60 parts),
The wax Czom>b was produced by melting and kneading talc (30 parts) and monoglyceride (20 parts) in a ladder at 20°C for 2 hours.

Chewing gum is made by adding the gum base, sugar sweetening ingredients, and glycerin to a preheated mixer using the prescription ingredients listed in the table, then kneading, adjusting the temperature so that it does not rise above 30℃, adding flavoring, and finally adding mutastein. A chewing gum composition was obtained and molded by kneading.

Example 6 Example/~j1 Control Example 1. We measured and compared the changes in mutastein activity of the chewing gums obtained with λ over time.

The EllK molded chewing gum was completely sealed with wax paper and aluminum laminated paper and left at room temperature, and the muta stain value was measured at the following intervals.

To measure the potency of mutastein, add distilled water≠M tube to chewing gum rg cut into approximately λ dragon angles, and crush with an ultrasonic crusher (for 2 minutes) to obtain an extract. Further, a small amount of distilled water is used to process and extract in the same manner, and the mixture is then centrifuged (3000 rpm, 6 minutes) together with the previous extract. Put the upper part into a /C volumetric flask and add 101ml of distilled water.
Then add 6jmM potassium phosphate buffer (P) to a test tube.
0.1 ml of the 2j sucrose solution (containing 0.0% sodium azide) prepared in I3.5) and the above extract θ~0.
trnl, distilled water 0. OJ' ~0. /♂ml, after adding glucashi synthase 0.02-, tilted at 30 degrees System 37
React for 6 hours at ℃. After the reaction, the solution in the test tube is discarded and the glucan adhering to the tube wall is washed with distilled water. After adding 3N of distilled water to the test tube and dispersing the glucan using an ultrasonic crusher, the absorption of ODjjOnm is measured using a spectrophotometer. The activity of mutastein is determined by the amount of mutastein that reduces the amount of insoluble glucan produced (ODjJOnm) by 50% in units of 1.

The measurement results are shown in the table below.

From the results in the above table, the residual potency rate of the control example using sugar as the sugar sweetening raw material was undefined, and the control example using sugar and glucose in combination was even worse, whereas the sugar according to the present invention Example/-1, in which biaxial species were used in combination, was good.

Example 7 Example 1-! The flavor quality of the chewing gums obtained in , Control Example/, - was compared.

In the test, 20 sensory inspectors tasted a sample of chewing gum C31 and conducted a comprehensive flavor comparison survey as a luxury item.

The evaluation criteria were: A: Very good flavor, B: Slightly good flavor, C+ fair, D: Slightly poor flavor, E: Very poor flavor.

The sensory test results are as shown in the table below.

From the results in the table above, it is shown that Examples 1-5 of the combination of sugar and other biaxes according to the present invention have superior flavor quality to the basic taste using sugar or sugar/glucose. .

〔Effect of the invention〕

According to the present invention, mutastin is stabilized over time,
In addition, it is possible to provide a low-cariogenic chewing gum composition containing Mutastin, which has a flavor superior to that of conventional chewing gums that use sugar as a sugar sweetening ingredient.

As mentioned above, the mutastein-containing chewing gum composition according to the present invention has been described with reference to preferred examples, but the present invention is not limited to these examples, and various changes can be made without departing from the spirit of the present invention. Of course, there are many things that can be done.

Patent applicant: Lotte Co., Ltd. Godo Shusei Co., Ltd.

Claims (9)

[Claims] (1) In a chewing gum composition consisting of gum paste raw materials, saccharide sweetening raw materials, and other chewing gum additive raw materials, the saccharide sweetening raw materials are mainly composed of sugar and one or more trisaccharides other than sugar, and physiologically active substances A mutastein-containing chewing gum composition characterized by containing mutastein. (2) Mutastein-containing chewing gum according to claim 1, wherein the trisaccharide other than sand m is a trisaccharide selected from the group consisting of maltose, isomaltose, palatinose, α-2-ctose, and β-2-ctose. Composition. (3) The amount of sugar and trisaccharides other than sugar in the chewing gum composition! The chewing gum composition containing mutastein according to claim 1 or 2, which contains 0 to 0% by weight. (4) The mutastein formulation according to any one of claims 1 to 3, wherein the combined amount of sugar and trisaccharide other than sugar is to-a'o weight % based on the chewing gum composition. Chewing gum composition. (5) The weight ratio of sugar and trisaccharides other than sugar is 2j ~
8. A chewing gum composition containing mutastein according to any one of claims 1 to 7. (6) The weight ratio of sugar and trisaccharides other than sugar is 60:11,
θ〜♂j:/! A chewing gum composition containing mutastein according to any one of claims 1 to 7. (7) The mutastein-containing chewing gum composition according to any one of claims 1 to 6, wherein the trisaccharides other than sugar are maltose and isomaltose. (8) The mutametein-containing chewing gum composition according to any one of claims 1 to 6, wherein the trisaccharide other than sugar is palatinose. (9) The mutastein-containing chewing gum composition according to claim 1, wherein the weight ratio of sugar and palatinose is 30 nia to ri/:ri. (The mutastein-containing chewing gum composition according to any one of claims 7 to 6, wherein the trisaccharides other than sugar 11 are maltose, isomaltose, and palatinose. The weight ratio of mixed disaccharide consisting of gas and palatinose is 2!r:
7j ~ J'J': Claim 1 which is /2
Mutastein-containing chewing gum composition according to item O.