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JPS6480295A - Beta-isopropylmalate dehydrogenase gene promoter and use thereof - Google Patents

Beta-isopropylmalate dehydrogenase gene promoter and use thereof

Info

Publication number
JPS6480295A
JPS6480295A JP535088A JP535088A JPS6480295A JP S6480295 A JPS6480295 A JP S6480295A JP 535088 A JP535088 A JP 535088A JP 535088 A JP535088 A JP 535088A JP S6480295 A JPS6480295 A JP S6480295A
Authority
JP
Japan
Prior art keywords
beta
gene
isopropylmalate dehydrogenase
acremonium chrysogenum
dehydrogenase gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP535088A
Other languages
Japanese (ja)
Inventor
Masaru Suzuki
Hiroyuki Kimura
Shigeo Matsumura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takeda Pharmaceutical Co Ltd
Original Assignee
Takeda Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Chemical Industries Ltd filed Critical Takeda Chemical Industries Ltd
Priority to JP535088A priority Critical patent/JPS6480295A/en
Publication of JPS6480295A publication Critical patent/JPS6480295A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To obtain a DNA fragment, having a promoter active part of beta- isopropylmalate dehydrogenase gene and capable of more effectively expressing heterogene in microbical cells of Acremonium chrysogenum. CONSTITUTION:A DNA containing gene of beta-isopropylmalate dehydrogenase (IPMDH) which is one of leucine biosynthetic enzymes is cloned from chromosomic DNA of Acremonium chrysogenum to analyze the structure of the gene. As a result, it is found that a region containing a promoter of IPMDH gene and translation starting part can be utilized for constructing useful expression vectors using Acremonium chrysogenum as a host.
JP535088A 1987-01-13 1988-01-12 Beta-isopropylmalate dehydrogenase gene promoter and use thereof Pending JPS6480295A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP535088A JPS6480295A (en) 1987-01-13 1988-01-12 Beta-isopropylmalate dehydrogenase gene promoter and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP724387 1987-01-13
JP535088A JPS6480295A (en) 1987-01-13 1988-01-12 Beta-isopropylmalate dehydrogenase gene promoter and use thereof

Publications (1)

Publication Number Publication Date
JPS6480295A true JPS6480295A (en) 1989-03-27

Family

ID=26339273

Family Applications (1)

Application Number Title Priority Date Filing Date
JP535088A Pending JPS6480295A (en) 1987-01-13 1988-01-12 Beta-isopropylmalate dehydrogenase gene promoter and use thereof

Country Status (1)

Country Link
JP (1) JPS6480295A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998037203A1 (en) * 1997-02-22 1998-08-27 Agrevo Uk Limited Fungicidal test method
WO2000011189A1 (en) * 1998-08-21 2000-03-02 Aventis Cropscience Uk Limited Use of leu a promoter of stagonospora nodorum in screening method for fungicides

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998037203A1 (en) * 1997-02-22 1998-08-27 Agrevo Uk Limited Fungicidal test method
WO2000011189A1 (en) * 1998-08-21 2000-03-02 Aventis Cropscience Uk Limited Use of leu a promoter of stagonospora nodorum in screening method for fungicides

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