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JPS6471480A - Cell line producing monoclonal antibody and monoclonal antibody produced therefrom - Google Patents

Cell line producing monoclonal antibody and monoclonal antibody produced therefrom

Info

Publication number
JPS6471480A
JPS6471480A JP22901087A JP22901087A JPS6471480A JP S6471480 A JPS6471480 A JP S6471480A JP 22901087 A JP22901087 A JP 22901087A JP 22901087 A JP22901087 A JP 22901087A JP S6471480 A JPS6471480 A JP S6471480A
Authority
JP
Japan
Prior art keywords
methamphetamine
monoclonal antibody
cell
cell line
derivative
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP22901087A
Other languages
Japanese (ja)
Inventor
Makoto Takeya
Kimimasa Miyazaki
Tadayasu Mitsumata
Hirokazu Sugihara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Panasonic Holdings Corp
Original Assignee
Matsushita Electric Industrial Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Matsushita Electric Industrial Co Ltd filed Critical Matsushita Electric Industrial Co Ltd
Priority to JP22901087A priority Critical patent/JPS6471480A/en
Publication of JPS6471480A publication Critical patent/JPS6471480A/en
Pending legal-status Critical Current

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To obtain a monoclonal antibody useful for the detection of methamphetamine, etc., by collecting an antibody-producing cell from a mouse immunized with a protein bonded with methamphetamine or a methamphetamine derivative and cloning the cell. CONSTITUTION:A complete antigen is prepared from a substance produced by bonding hemocyanine originated from false impet (Macroschisma sinensis) and methamphetamine or a methamphetamine derivative. The antigen is mixed with Freund's complete adjuvant containing dead cell of human tubercle bacillus and a Ba1b/c mouse is immunized with the mixture. The spleen cell of the animal is extracted and fused with a cell line p3X63-Ag8.653 originated from myeloma. A cell line exhibiting high titer against methamphetamine and high proliferation property is selected from the fused cells by ELISA method. A monoclonal antibody specifically linking with methamphetamine or a methamphetamine derivative can be obtained from the cell line.
JP22901087A 1987-09-11 1987-09-11 Cell line producing monoclonal antibody and monoclonal antibody produced therefrom Pending JPS6471480A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP22901087A JPS6471480A (en) 1987-09-11 1987-09-11 Cell line producing monoclonal antibody and monoclonal antibody produced therefrom

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP22901087A JPS6471480A (en) 1987-09-11 1987-09-11 Cell line producing monoclonal antibody and monoclonal antibody produced therefrom

Publications (1)

Publication Number Publication Date
JPS6471480A true JPS6471480A (en) 1989-03-16

Family

ID=16885343

Family Applications (1)

Application Number Title Priority Date Filing Date
JP22901087A Pending JPS6471480A (en) 1987-09-11 1987-09-11 Cell line producing monoclonal antibody and monoclonal antibody produced therefrom

Country Status (1)

Country Link
JP (1) JPS6471480A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0311383A2 (en) * 1987-10-09 1989-04-12 Ube Industries, Ltd. Monoclonal antibody to methamphetamine, preparation of the same, assay method and assay kit of methamphetamine
KR20030080782A (en) * 2002-04-10 2003-10-17 태건식 The mass preparation method of monoclonal antibodies to methamphetamine

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0311383A2 (en) * 1987-10-09 1989-04-12 Ube Industries, Ltd. Monoclonal antibody to methamphetamine, preparation of the same, assay method and assay kit of methamphetamine
KR20030080782A (en) * 2002-04-10 2003-10-17 태건식 The mass preparation method of monoclonal antibodies to methamphetamine

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