[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

JPS6443194A - Plasmid and escherichia-coli transformed therewith - Google Patents

Plasmid and escherichia-coli transformed therewith

Info

Publication number
JPS6443194A
JPS6443194A JP20052487A JP20052487A JPS6443194A JP S6443194 A JPS6443194 A JP S6443194A JP 20052487 A JP20052487 A JP 20052487A JP 20052487 A JP20052487 A JP 20052487A JP S6443194 A JPS6443194 A JP S6443194A
Authority
JP
Japan
Prior art keywords
plasmid
thermostable
mentioned
dna
afore
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP20052487A
Other languages
Japanese (ja)
Other versions
JP2602840B2 (en
Inventor
Yonekazu Sakamoto
Masao Kageyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unitika Ltd
Original Assignee
Unitika Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unitika Ltd filed Critical Unitika Ltd
Priority to JP20052487A priority Critical patent/JP2602840B2/en
Publication of JPS6443194A publication Critical patent/JPS6443194A/en
Application granted granted Critical
Publication of JP2602840B2 publication Critical patent/JP2602840B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To produce a thermostable alanine dehydrogenase, by linking a thermostable dehydrogenase gene having a specific mol. wt. or below to a vector plasmid, introducing the resultant recombinant plasmid into Escherichia coli and cultivating the obtained transformant strain in a culture medium. CONSTITUTION:A chromosomic DNA of Bacillus.sterothermophilus IFO12550 strain is digested with a restriction enzyme SalI to provide a DNA fragment, which is then linked to a plasmid pBR322 treated with the SalI with a T4 ligase to afford a plasmid pICR3. An exogenote region of the above-mentioned plasmid is subsequently taken out and introduced into an M13 phage vector to determine a DNA base sequence. A DNA fragment, capable of coding an alanine dehydrogenase and having <=2 megadaltons is obtained on the basis of the afore-mentioned sequence and linked to a plasmid pKK223-3 by ligase treatment to afford pICR330. The afore-mentioned plasmid is then introduced into Escherichia coli and the resultant transformant strain is cultivated in a culture medium to provide the aimed thermostable enzyme.
JP20052487A 1987-08-10 1987-08-10 Plasmids and Escherichia coli transformed therewith Expired - Lifetime JP2602840B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP20052487A JP2602840B2 (en) 1987-08-10 1987-08-10 Plasmids and Escherichia coli transformed therewith

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP20052487A JP2602840B2 (en) 1987-08-10 1987-08-10 Plasmids and Escherichia coli transformed therewith

Publications (2)

Publication Number Publication Date
JPS6443194A true JPS6443194A (en) 1989-02-15
JP2602840B2 JP2602840B2 (en) 1997-04-23

Family

ID=16425743

Family Applications (1)

Application Number Title Priority Date Filing Date
JP20052487A Expired - Lifetime JP2602840B2 (en) 1987-08-10 1987-08-10 Plasmids and Escherichia coli transformed therewith

Country Status (1)

Country Link
JP (1) JP2602840B2 (en)

Also Published As

Publication number Publication date
JP2602840B2 (en) 1997-04-23

Similar Documents

Publication Publication Date Title
WO2000018906A3 (en) Shuffling of codon altered genes
DK181286D0 (en) MUTANTS OF HUMAN WEAVE PLASMINOGEN ACTIVATOR (T-PA), DNA SEQUENCES THAT CODE THESE, EXPRESSION VECTORS THAT CAN EXPRESS THE DNA SEQUENCES, MICROORGANISMS AND CELL CULTURES OF THE TRANSFORMER TRANSFORMERS.
DK0724642T4 (en) Nucleic acid vector, composition containing such vector and vaccine for immunization against hepatitis
AU1672388A (en) Expression of human proapolipoprotein a-i
CS9100531A2 (en) Dna fragment that codes polypeptide with nitrile-hydratation activity,transformant that contains this gene and method of amides preparation with this transformant application
Toba et al. Colicin E8, a DNase which indicates an evolutionary relationship between colicins E2 and E3
MY127754A (en) Plasmid autonomously replicable in coryneform bacteria.
CA2252493A1 (en) .beta.-galactoside - .alpha. 2,6-sialyltransferase gene
AU577810B2 (en) Cloning the gene for rabbit tumor necrosis factor polypeptide
GR3022085T3 (en) Protein with urate oxidase activity, recombinant gene coding therefor, expression vector, micro-organisms and transformed cells
HUT68379A (en) Method for modifying the cell, tissue or host tropism of a recombinant microorganism obtained this way and use thereof in medicine and veterinary medicine
AU1778988A (en) Novel fibrinolytic enzymes
JPS6443194A (en) Plasmid and escherichia-coli transformed therewith
EP1277837A3 (en) Epididymis-specific DNA sequences and their use
AU7949991A (en) Cloned n-methylhydantoinase
ES2003085A6 (en) An improved heat-shock control method and system for the production of competent eukaryotic gene products.
JPS6439990A (en) Fused gene by bonding aequorin gene to functional gene
ATE120491T1 (en) BALANCED CONSTITUTIVE INDUCIBLE TRANSCRIPTION SYSTEM.
EP1688491A4 (en) Novel plasmids and utilization thereof
O'Hare et al. Production in Escherichia coli of porcine type-I collagenase as a fusion protein with β-galactosidase
NZ505350A (en) DNA encoding a tumour antigen peptide (43 kDa) for diagnosis or treatment of tumours
Sato et al. IS421, a new insertion sequence in Escherichia coli
NO301174B1 (en) Process for preparing an improved therapeutically active tissue plasminogen activator
Fortnagel et al. Sequence homologies of glucose-dehydrogenases of Bacillus megaterium and Bacillus subtilis
JPS6474991A (en) Thermostable pullulanase gene dna, recombinant dna containing said dna and transformant