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JPS6429399A - Method for separating human lymphotoxin - Google Patents

Method for separating human lymphotoxin

Info

Publication number
JPS6429399A
JPS6429399A JP18496987A JP18496987A JPS6429399A JP S6429399 A JPS6429399 A JP S6429399A JP 18496987 A JP18496987 A JP 18496987A JP 18496987 A JP18496987 A JP 18496987A JP S6429399 A JPS6429399 A JP S6429399A
Authority
JP
Japan
Prior art keywords
human lymphotoxin
lymphotoxin
adsorbed
sulfate
aqueous solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP18496987A
Other languages
Japanese (ja)
Inventor
Kazuhiko Arai
Morio Niimura
Tooru Mitsuno
Hiroyasu Suzuki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Denka Co Ltd
Original Assignee
Denki Kagaku Kogyo KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Denki Kagaku Kogyo KK filed Critical Denki Kagaku Kogyo KK
Priority to JP18496987A priority Critical patent/JPS6429399A/en
Publication of JPS6429399A publication Critical patent/JPS6429399A/en
Pending legal-status Critical Current

Links

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PURPOSE:To separate the titled compound useful as an antitumor agent as a single component, by adsorbing a mixture consisting of human lymphotoxin and a partial hydrolyzate thereof on specific polysaccharides and desorbing the adsorbed lymphotoxin with an aqueous solution of an inorganic salt. CONSTITUTION:A mixture consisting of human lymphotoxin and a partial hydrolyzate thereof is adsorbed on water-insoluble polysaccharides (e.g. cellulose sulfate, agarose sulfate or crosslinked dextran sulfate) having linked sulfuric acid groups. The adsorbed human lymphotoxin is then desorbed with an aqueous solution of an inorganic salt to separate the aimed human lymphotoxin.
JP18496987A 1987-07-24 1987-07-24 Method for separating human lymphotoxin Pending JPS6429399A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP18496987A JPS6429399A (en) 1987-07-24 1987-07-24 Method for separating human lymphotoxin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP18496987A JPS6429399A (en) 1987-07-24 1987-07-24 Method for separating human lymphotoxin

Publications (1)

Publication Number Publication Date
JPS6429399A true JPS6429399A (en) 1989-01-31

Family

ID=16162521

Family Applications (1)

Application Number Title Priority Date Filing Date
JP18496987A Pending JPS6429399A (en) 1987-07-24 1987-07-24 Method for separating human lymphotoxin

Country Status (1)

Country Link
JP (1) JPS6429399A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0592989A2 (en) * 1992-10-12 1994-04-20 B. Braun Melsungen Ag Process of the selective and quantitative removal or preparation of tumor necrosis factor (TNF) and/or lipopolysaccharides (LPS) of aqueous solutions

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0592989A2 (en) * 1992-10-12 1994-04-20 B. Braun Melsungen Ag Process of the selective and quantitative removal or preparation of tumor necrosis factor (TNF) and/or lipopolysaccharides (LPS) of aqueous solutions
EP0592989A3 (en) * 1992-10-12 1995-12-13 Braun Melsungen Ag Process of the selective and quantitative removal or preparation of tumor necrosis factor (tnf) and/or lipopolysaccharides (lps) of aqueous solutions

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