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JPS6420099A - Method for determining free fatty acid and reagent composition for determining free fatty acid useful in said determination - Google Patents

Method for determining free fatty acid and reagent composition for determining free fatty acid useful in said determination

Info

Publication number
JPS6420099A
JPS6420099A JP17492787A JP17492787A JPS6420099A JP S6420099 A JPS6420099 A JP S6420099A JP 17492787 A JP17492787 A JP 17492787A JP 17492787 A JP17492787 A JP 17492787A JP S6420099 A JPS6420099 A JP S6420099A
Authority
JP
Japan
Prior art keywords
fatty acid
free fatty
determining free
acylcoenzyme
determination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP17492787A
Other languages
Japanese (ja)
Other versions
JP2704616B2 (en
Inventor
Shin Sugou
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eiken Chemical Co Ltd
Original Assignee
Eiken Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eiken Chemical Co Ltd filed Critical Eiken Chemical Co Ltd
Priority to JP17492787A priority Critical patent/JP2704616B2/en
Publication of JPS6420099A publication Critical patent/JPS6420099A/en
Application granted granted Critical
Publication of JP2704616B2 publication Critical patent/JP2704616B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To measure a free fatty acid in high precise and sensitivity, by reacting a substance other than straight-chain fatty acid with coenzyme A and acylcoenzyme A oxidase and measuring the change after the reaction. CONSTITUTION:A free fatty acid other than a straight-chain fatty acid is reacted with an acylcoenzyme A synthetic enzyme in the presence of adenosine 3-phosphoric acid and coenzyme A to provide an acylcoenzyme A, which is reacted with an acylcoenzyme A oxidase at pH6.0-8.5 to oxidize the acylcoenzyme A and peroxidase, takarase, etc., is added to the resultant H2O2 to provide a pigment, which is then subjected to colorimetric determination.
JP17492787A 1987-07-15 1987-07-15 Free fatty acid determination method and reagent composition for free fatty acid determination useful for the determination method Expired - Lifetime JP2704616B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP17492787A JP2704616B2 (en) 1987-07-15 1987-07-15 Free fatty acid determination method and reagent composition for free fatty acid determination useful for the determination method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP17492787A JP2704616B2 (en) 1987-07-15 1987-07-15 Free fatty acid determination method and reagent composition for free fatty acid determination useful for the determination method

Publications (2)

Publication Number Publication Date
JPS6420099A true JPS6420099A (en) 1989-01-24
JP2704616B2 JP2704616B2 (en) 1998-01-26

Family

ID=15987151

Family Applications (1)

Application Number Title Priority Date Filing Date
JP17492787A Expired - Lifetime JP2704616B2 (en) 1987-07-15 1987-07-15 Free fatty acid determination method and reagent composition for free fatty acid determination useful for the determination method

Country Status (1)

Country Link
JP (1) JP2704616B2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0771760A2 (en) 1995-11-02 1997-05-07 Fuji Photo Film Co., Ltd. Process for the purification of aluminum hydroxide and process for the surface treatment of aluminum plate utilizing the purification process
US6320124B1 (en) * 1999-10-26 2001-11-20 Veterans General Hospital - Taipei Electrophysiological faraday box
JP2002051769A (en) * 2000-08-11 2002-02-19 Azwell Inc Liquid reagent useful for measuring nonesterified fatty acid

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0771760A2 (en) 1995-11-02 1997-05-07 Fuji Photo Film Co., Ltd. Process for the purification of aluminum hydroxide and process for the surface treatment of aluminum plate utilizing the purification process
US6320124B1 (en) * 1999-10-26 2001-11-20 Veterans General Hospital - Taipei Electrophysiological faraday box
JP2002051769A (en) * 2000-08-11 2002-02-19 Azwell Inc Liquid reagent useful for measuring nonesterified fatty acid

Also Published As

Publication number Publication date
JP2704616B2 (en) 1998-01-26

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