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JPS6416584A - Preparation of aqueous solution of tryptophan synthase - Google Patents

Preparation of aqueous solution of tryptophan synthase

Info

Publication number
JPS6416584A
JPS6416584A JP62172863A JP17286387A JPS6416584A JP S6416584 A JPS6416584 A JP S6416584A JP 62172863 A JP62172863 A JP 62172863A JP 17286387 A JP17286387 A JP 17286387A JP S6416584 A JPS6416584 A JP S6416584A
Authority
JP
Japan
Prior art keywords
enzyme
aqueous solution
heat
tryptophan synthase
suspension
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP62172863A
Other languages
Japanese (ja)
Inventor
Shoichiro Miyahara
Toshio Matsumoto
Makoto Yanaka
Kazunari Nitta
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsui Toatsu Chemicals Inc
Original Assignee
Mitsui Toatsu Chemicals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsui Toatsu Chemicals Inc filed Critical Mitsui Toatsu Chemicals Inc
Priority to JP62172863A priority Critical patent/JPS6416584A/en
Priority to KR1019880008652A priority patent/KR900005773B1/en
Priority to AU18985/88A priority patent/AU613057B2/en
Priority to EP88306349A priority patent/EP0299715A3/en
Priority to BR888803497A priority patent/BR8803497A/en
Priority to DK388888A priority patent/DK388888A/en
Publication of JPS6416584A publication Critical patent/JPS6416584A/en
Pending legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE:To obtain the titled enzyme in the form of an aqueous solution, by culturing a microbial strain containing the titled enzyme, disintegrating the microbial cells at a low temperature, extracting the enzyme, heat-treating in the presence of sodium chloride or L-serine and removing residue and other proteins from the product. CONSTITUTION:A microbial strain such as Escherichia coli MT-10242 (FERM BP-20) is cultured in a medium containing a carbon source, a nitrogen source, etc., and the microbial cells in the cultured liquid are mechanically disintegrated at a cell concentration of 30-60g/l at pH 6-10 and <=30 deg.C, preferably 5-15 deg.C. After the disintegration treatment, the obtained suspension containing tryptophan synthase transferred from the cell is added with sodium chloride or L-serine and heat-treated at 40-45 deg.C in the presence of the above additive. The heat- treated suspension is immediately cooled to <=30 deg.C and the residue of disintegrated cells and precipitated proteins other than tryptophan synthase are removed from the suspension to obtain the objective aqueous solution of the enzyme.
JP62172863A 1987-07-13 1987-07-13 Preparation of aqueous solution of tryptophan synthase Pending JPS6416584A (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP62172863A JPS6416584A (en) 1987-07-13 1987-07-13 Preparation of aqueous solution of tryptophan synthase
KR1019880008652A KR900005773B1 (en) 1987-07-13 1988-07-12 Process for preparing l-tryptophan and process for preparing a stable agueous enzyme solution
AU18985/88A AU613057B2 (en) 1987-07-13 1988-07-12 Process for preparing L-tryprophan and process for preparing a stable aqueous enzyme solution
EP88306349A EP0299715A3 (en) 1987-07-13 1988-07-12 Process for preparing L-tryptophan
BR888803497A BR8803497A (en) 1987-07-13 1988-07-12 PROCESS FOR THE PREPARATION OF L-TRYPTOPHANE AND PROCESS FOR THE PREPARATION OF AQUEOUS ENZYMATIC SOLUTION OF TRIPTOPHANE SYNTHESIS OUTRIPTOPHANESE
DK388888A DK388888A (en) 1987-07-13 1988-07-12 PROCEDURE FOR PREPARING L-TRYPTOPHAN AND PROCEDURE FOR PREPARING A STABLE Aqueous Enzyme Solution

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62172863A JPS6416584A (en) 1987-07-13 1987-07-13 Preparation of aqueous solution of tryptophan synthase

Publications (1)

Publication Number Publication Date
JPS6416584A true JPS6416584A (en) 1989-01-20

Family

ID=15949692

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62172863A Pending JPS6416584A (en) 1987-07-13 1987-07-13 Preparation of aqueous solution of tryptophan synthase

Country Status (1)

Country Link
JP (1) JPS6416584A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5065193A (en) * 1989-05-24 1991-11-12 Onoda Cement Co., Ltd. Heat fixing roll for copying machine, method of producing the same and electronic copying machine provided with the same

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5065193A (en) * 1989-05-24 1991-11-12 Onoda Cement Co., Ltd. Heat fixing roll for copying machine, method of producing the same and electronic copying machine provided with the same

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