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JPS5776455A - Novel method for detection and determination of amino acid and/or amine - Google Patents

Novel method for detection and determination of amino acid and/or amine

Info

Publication number
JPS5776455A
JPS5776455A JP15149980A JP15149980A JPS5776455A JP S5776455 A JPS5776455 A JP S5776455A JP 15149980 A JP15149980 A JP 15149980A JP 15149980 A JP15149980 A JP 15149980A JP S5776455 A JPS5776455 A JP S5776455A
Authority
JP
Japan
Prior art keywords
sample
amino acids
test tube
amine
nbd
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP15149980A
Other languages
Japanese (ja)
Other versions
JPS6322261B2 (en
Inventor
Kazuhiro Imai
Yoshihiko Watanabe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP15149980A priority Critical patent/JPS5776455A/en
Publication of JPS5776455A publication Critical patent/JPS5776455A/en
Publication of JPS6322261B2 publication Critical patent/JPS6322261B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

PURPOSE:To detect and determine amino acids and/or amines with high sensitivity by adding a fluorescence emissive reagent NBD-F into the sample to be tested to cause the sample to react and measuring the fluorescence intensity of the deriv. produced at this time. CONSTITUTION:A sample to be tested contg. amino acids and a 0.1M boric acid buffer contg. 0.1ml NBD-F enthanol soln. and 20% ethanol are sampled in a test tube and are mixed. This test tube is put in a thermostatic chamber controlled to 60+ or -1 deg.C, to cause the amino acids or amine and the fluorescence emissive reagent NBD-F to react. Upon completion of the reaction, the test tube is cooled with iced water, after which the fluorescence intensity is measured by using a fluorophotometer. Thereby, the usage of the reagent and the sample is reduced and reaction time and reaction temp. are reduced.
JP15149980A 1980-10-30 1980-10-30 Novel method for detection and determination of amino acid and/or amine Granted JPS5776455A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP15149980A JPS5776455A (en) 1980-10-30 1980-10-30 Novel method for detection and determination of amino acid and/or amine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP15149980A JPS5776455A (en) 1980-10-30 1980-10-30 Novel method for detection and determination of amino acid and/or amine

Publications (2)

Publication Number Publication Date
JPS5776455A true JPS5776455A (en) 1982-05-13
JPS6322261B2 JPS6322261B2 (en) 1988-05-11

Family

ID=15519835

Family Applications (1)

Application Number Title Priority Date Filing Date
JP15149980A Granted JPS5776455A (en) 1980-10-30 1980-10-30 Novel method for detection and determination of amino acid and/or amine

Country Status (1)

Country Link
JP (1) JPS5776455A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0481665A (en) * 1990-03-29 1992-03-16 Shimadzu Corp Method for analyzing bioamino acid
EP1542017A1 (en) * 2002-07-04 2005-06-15 Mitsubishi Pharma Corporation Method of examining and diagnosing integration dysfunction syndrome

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0613962U (en) * 1992-07-30 1994-02-22 三菱農機株式会社 Tractor safety frame

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0481665A (en) * 1990-03-29 1992-03-16 Shimadzu Corp Method for analyzing bioamino acid
EP1542017A1 (en) * 2002-07-04 2005-06-15 Mitsubishi Pharma Corporation Method of examining and diagnosing integration dysfunction syndrome
EP1542017A4 (en) * 2002-07-04 2007-07-11 Mitsubishi Pharma Corp Method of examining and diagnosing integration dysfunction syndrome

Also Published As

Publication number Publication date
JPS6322261B2 (en) 1988-05-11

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