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JPS57207861A - Measuring method for dyeing concentration of cultured cell and vessel for measurement - Google Patents

Measuring method for dyeing concentration of cultured cell and vessel for measurement

Info

Publication number
JPS57207861A
JPS57207861A JP9345281A JP9345281A JPS57207861A JP S57207861 A JPS57207861 A JP S57207861A JP 9345281 A JP9345281 A JP 9345281A JP 9345281 A JP9345281 A JP 9345281A JP S57207861 A JPS57207861 A JP S57207861A
Authority
JP
Japan
Prior art keywords
cultured cell
vessel
dyeing
cell
measuring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP9345281A
Other languages
Japanese (ja)
Inventor
Sachiko Tachikawa
Masao Izawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Olympus Corp
Olympus Optical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corp, Olympus Optical Co Ltd filed Critical Olympus Corp
Priority to JP9345281A priority Critical patent/JPS57207861A/en
Publication of JPS57207861A publication Critical patent/JPS57207861A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To measure proper dyed density of cultured cell etc. without taking out the cultured cell, by immersing an indication body having the sensitivity for dyeing liquid into the dyeing liquid of the cultured cell etc. or the dyeing liquid corresponding to the dyeing liquid and measuring the secular dyed density of the indication body. CONSTITUTION:An indication body 12 is made by a high molecular film, a piece of paper etc. uniformly applied or stuck cytoplasm-like synthetic protein corresponding to vinyl chloride or vinyl acetate synthetic resin having the sensitivity for a dying agent of a cultured cell 13 or the cultured cell and said body 12 is attached to the inner wall of a culture vessel-measuring vessel 10. A dyeing agent 15 is dissolved to the liquid in the vessel 10 and the dyed density of the cell is measured continuously and automatically by measuring the quantity of transmitted light at the time of transmitting light from a luminous source such as a light emission diode 16 etc. through the body 12 by a photodetecting element 17 arranged on the outer wall of a vessel 10 as the dyed density accompanied by the secular change. In this manner, proper dyeing time is known without taking out the cell on a bottom face 14.
JP9345281A 1981-06-17 1981-06-17 Measuring method for dyeing concentration of cultured cell and vessel for measurement Pending JPS57207861A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP9345281A JPS57207861A (en) 1981-06-17 1981-06-17 Measuring method for dyeing concentration of cultured cell and vessel for measurement

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP9345281A JPS57207861A (en) 1981-06-17 1981-06-17 Measuring method for dyeing concentration of cultured cell and vessel for measurement

Publications (1)

Publication Number Publication Date
JPS57207861A true JPS57207861A (en) 1982-12-20

Family

ID=14082712

Family Applications (1)

Application Number Title Priority Date Filing Date
JP9345281A Pending JPS57207861A (en) 1981-06-17 1981-06-17 Measuring method for dyeing concentration of cultured cell and vessel for measurement

Country Status (1)

Country Link
JP (1) JPS57207861A (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0333253A2 (en) * 1988-03-15 1989-09-20 Akzo Nobel N.V. Apparatus and device for detecting microorganisms
US5094955A (en) * 1988-03-15 1992-03-10 Akzo N.V. Device and method for detecting microorganisms
US5162229A (en) * 1988-03-15 1992-11-10 Akzo N.V. Device and method for enhanced recovery and detection of microbial growth in the presence of antimicrobial substances
US5164796A (en) * 1988-03-15 1992-11-17 Akzo N.V. Apparatus and method for detection of microorganisms
US5217875A (en) * 1989-05-12 1993-06-08 Avl Ag Method for detecting biological activities in a specimen and a device for implementing the method
US5217876A (en) * 1988-03-15 1993-06-08 Akzo N.V. Method for detecting microorganisms
US5518895A (en) * 1990-02-15 1996-05-21 Akzo N.V. Device for detecting microorganisms using piezoelectric means
US5858769A (en) * 1989-05-15 1999-01-12 Akzo Nobel N.V. Device for detecting microorganisms

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0333253A2 (en) * 1988-03-15 1989-09-20 Akzo Nobel N.V. Apparatus and device for detecting microorganisms
US5094955A (en) * 1988-03-15 1992-03-10 Akzo N.V. Device and method for detecting microorganisms
US5162229A (en) * 1988-03-15 1992-11-10 Akzo N.V. Device and method for enhanced recovery and detection of microbial growth in the presence of antimicrobial substances
US5164796A (en) * 1988-03-15 1992-11-17 Akzo N.V. Apparatus and method for detection of microorganisms
AU631533B2 (en) * 1988-03-15 1992-12-03 Biomerieux, Inc. Apparatus and device for detecting microorganisms
US5217876A (en) * 1988-03-15 1993-06-08 Akzo N.V. Method for detecting microorganisms
US5856175A (en) * 1988-03-15 1999-01-05 Akzo Nobel N.V. Device for detecting microorganisms
US5217875A (en) * 1989-05-12 1993-06-08 Avl Ag Method for detecting biological activities in a specimen and a device for implementing the method
US5858769A (en) * 1989-05-15 1999-01-12 Akzo Nobel N.V. Device for detecting microorganisms
US5518895A (en) * 1990-02-15 1996-05-21 Akzo N.V. Device for detecting microorganisms using piezoelectric means

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