JPS5693046A - Determination of beta2-microglobulin by enzime immunity measuring method - Google Patents
Determination of beta2-microglobulin by enzime immunity measuring methodInfo
- Publication number
- JPS5693046A JPS5693046A JP14957679A JP14957679A JPS5693046A JP S5693046 A JPS5693046 A JP S5693046A JP 14957679 A JP14957679 A JP 14957679A JP 14957679 A JP14957679 A JP 14957679A JP S5693046 A JPS5693046 A JP S5693046A
- Authority
- JP
- Japan
- Prior art keywords
- microglobulin
- bead
- antibody
- human immune
- enzime
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000015736 beta 2-Microglobulin Human genes 0.000 title 1
- 108010081355 beta 2-Microglobulin Proteins 0.000 title 1
- 230000036039 immunity Effects 0.000 title 1
- 239000011324 bead Substances 0.000 abstract 5
- 102000003992 Peroxidases Human genes 0.000 abstract 3
- 239000007788 liquid Substances 0.000 abstract 3
- 108040007629 peroxidase activity proteins Proteins 0.000 abstract 3
- 239000000427 antigen Substances 0.000 abstract 2
- 102000036639 antigens Human genes 0.000 abstract 2
- 108091007433 antigens Proteins 0.000 abstract 2
- 230000001235 sensitizing effect Effects 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- 210000002381 plasma Anatomy 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 210000002700 urine Anatomy 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To accomplish a determination at a high accuracy with an inexpensive facility and easy handleability by employing a polystylene bead sensitizing a human immune β2-microglobulin antibody and a peroxidase based human immune microglobulin antibody.
CONSTITUTION: A polystylene bead sensitizing a human immune β2-microglobulin is dipped into a dilute of a test piece such as serum, plasma and urine, shaked and allowed to stand. β2-microglobulin (antigen) is connected to the human immune β2-microglobulin fixed on the bead left undissolved in a liquid being tested. After the bead is washed, it is immersed in a liquid containing a peroxidase based human immune β2-microglobulin. In this manner, an enzime based antibody is connected to the antigen connected to the antibody on the bead. After excessive enzime based antibody is washed away, the content of the β2-microglobulin in the liquid being tested undergoes colorimetric determination with the oxidation effect of peroxidase as an indicator.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14957679A JPS5693046A (en) | 1979-11-20 | 1979-11-20 | Determination of beta2-microglobulin by enzime immunity measuring method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14957679A JPS5693046A (en) | 1979-11-20 | 1979-11-20 | Determination of beta2-microglobulin by enzime immunity measuring method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5693046A true JPS5693046A (en) | 1981-07-28 |
Family
ID=15478206
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14957679A Pending JPS5693046A (en) | 1979-11-20 | 1979-11-20 | Determination of beta2-microglobulin by enzime immunity measuring method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5693046A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4770774A (en) * | 1985-09-19 | 1988-09-13 | Toray Industries, Inc. | Column for removing β2 -microglobulin |
-
1979
- 1979-11-20 JP JP14957679A patent/JPS5693046A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4770774A (en) * | 1985-09-19 | 1988-09-13 | Toray Industries, Inc. | Column for removing β2 -microglobulin |
| JPH06104122B1 (en) * | 1985-09-19 | 1994-12-21 |
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