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JPS5639797A - Analytical method of bile acid and apparatus for the same - Google Patents

Analytical method of bile acid and apparatus for the same

Info

Publication number
JPS5639797A
JPS5639797A JP11539079A JP11539079A JPS5639797A JP S5639797 A JPS5639797 A JP S5639797A JP 11539079 A JP11539079 A JP 11539079A JP 11539079 A JP11539079 A JP 11539079A JP S5639797 A JPS5639797 A JP S5639797A
Authority
JP
Japan
Prior art keywords
immobilized
sample
reaction
column
bile acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP11539079A
Other languages
Japanese (ja)
Other versions
JPS5638200B2 (en
Inventor
Sakae Toudate
Koichi Mochizuki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jasco Corp
Original Assignee
Japan Spectroscopic Co Ltd
Nihon Bunko Kogyo KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Spectroscopic Co Ltd, Nihon Bunko Kogyo KK filed Critical Japan Spectroscopic Co Ltd
Priority to JP11539079A priority Critical patent/JPS5639797A/en
Publication of JPS5639797A publication Critical patent/JPS5639797A/en
Publication of JPS5638200B2 publication Critical patent/JPS5638200B2/ja
Granted legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To make it possible to analyze both unconjugated and conjugated bile acids directly and simultaneously, by using a column containing immobilized 3α-hydroxysteroid dehydrogenase.
CONSTITUTION: Immobilized 3α-hydroxysteroid dehydrogenase (3α-HSD) is immobilized in a porous carrier and held in a reaction coumn 8. A pretreated sample is injected into a separation column 5 and separated with a liquid fed from a gradient device 2 by a pump 3, and then eluted. A soution containing NAD+ (nicotinamide adenine dinucleotid) is fed from a reaction liquid reservoir 6 by a pump 7, mixed with the sample and reacted with the bile acid components in the sample in the reaction column 8. The resultant keto derivative is analyzed by ultraviolet absorption or fluorometric analysis in a detector 9 in order.
COPYRIGHT: (C)1981,JPO&Japio
JP11539079A 1979-09-07 1979-09-07 Analytical method of bile acid and apparatus for the same Granted JPS5639797A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP11539079A JPS5639797A (en) 1979-09-07 1979-09-07 Analytical method of bile acid and apparatus for the same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP11539079A JPS5639797A (en) 1979-09-07 1979-09-07 Analytical method of bile acid and apparatus for the same

Publications (2)

Publication Number Publication Date
JPS5639797A true JPS5639797A (en) 1981-04-15
JPS5638200B2 JPS5638200B2 (en) 1981-09-04

Family

ID=14661353

Family Applications (1)

Application Number Title Priority Date Filing Date
JP11539079A Granted JPS5639797A (en) 1979-09-07 1979-09-07 Analytical method of bile acid and apparatus for the same

Country Status (1)

Country Link
JP (1) JPS5639797A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57194351A (en) * 1981-05-26 1982-11-29 Shimadzu Corp Bile acid analysing method
JPS5961777A (en) * 1982-09-30 1984-04-09 Shimadzu Corp Analytical system of bile acid
CN104155438A (en) * 2014-09-09 2014-11-19 湖北科技学院 Determination reagent of total bile acid
CN105301255A (en) * 2014-04-18 2016-02-03 安徽大千生物工程有限公司 Preparation method of kit for measuring glycocholic acid content in human body
CN105588936A (en) * 2015-12-14 2016-05-18 浙江达美生物技术有限公司 Determination reagent for glycocholic acid and preparation method of determination reagent

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5444597A (en) * 1977-09-14 1979-04-09 Shimadzu Corp Measuring method of bile acids

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5444597A (en) * 1977-09-14 1979-04-09 Shimadzu Corp Measuring method of bile acids

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57194351A (en) * 1981-05-26 1982-11-29 Shimadzu Corp Bile acid analysing method
JPS6230588B2 (en) * 1981-05-26 1987-07-03 Shimadzu Corp
JPS5961777A (en) * 1982-09-30 1984-04-09 Shimadzu Corp Analytical system of bile acid
CN105301255A (en) * 2014-04-18 2016-02-03 安徽大千生物工程有限公司 Preparation method of kit for measuring glycocholic acid content in human body
CN104155438A (en) * 2014-09-09 2014-11-19 湖北科技学院 Determination reagent of total bile acid
CN104155438B (en) * 2014-09-09 2016-04-06 湖北科技学院 A kind of mensuration reagent of TBA
CN105588936A (en) * 2015-12-14 2016-05-18 浙江达美生物技术有限公司 Determination reagent for glycocholic acid and preparation method of determination reagent

Also Published As

Publication number Publication date
JPS5638200B2 (en) 1981-09-04

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