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JPS5633550A - Latex reagent sensitized with hemolytic streptococcal nicotinamide adenine dinucleotidase (nadase), method of producing the same and method of detecting nadase antibody using the same - Google Patents

Latex reagent sensitized with hemolytic streptococcal nicotinamide adenine dinucleotidase (nadase), method of producing the same and method of detecting nadase antibody using the same

Info

Publication number
JPS5633550A
JPS5633550A JP10910679A JP10910679A JPS5633550A JP S5633550 A JPS5633550 A JP S5633550A JP 10910679 A JP10910679 A JP 10910679A JP 10910679 A JP10910679 A JP 10910679A JP S5633550 A JPS5633550 A JP S5633550A
Authority
JP
Japan
Prior art keywords
nadase
same
latex
dinucleotidase
antibody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP10910679A
Other languages
Japanese (ja)
Inventor
Tetsuo Tomiyama
Taku Ogura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seikagaku Corp
Original Assignee
Seikagaku Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seikagaku Corp filed Critical Seikagaku Corp
Priority to JP10910679A priority Critical patent/JPS5633550A/en
Priority to GB8027769A priority patent/GB2058083B/en
Priority to DE19803032464 priority patent/DE3032464A1/en
Publication of JPS5633550A publication Critical patent/JPS5633550A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PURPOSE: To produce the subject reagent capable of detecting a nicotinamide adenine dinucleotidase (NADase) antibody with high sensitivity, conveiniently and easily and at cheap cost, by sensitizing latex particles with hemolytic streptococcal NADase.
CONSTITUTION: Particles of synthetic high-molecular latex are sensitized with NADase purified from a hemolytic streptococcal cultivation filtrate to produce a sensitized latex reagent. In the sensitization, latex particles are brought into contact with the antigen in an aqueous medium such as water, or a physiological saline solution, and left standstill, agitated or shaked. After the sensitization, the latex particles are washed with the aqueous medium to eliminate surplus antigen. The thus obtained latex reagent is stable, but it can be preserved for a long period of time by, for example, freeze-drying. The freeze-dried article is dissolved by adding a diluent, when used.
COPYRIGHT: (C)1981,JPO&Japio
JP10910679A 1979-08-29 1979-08-29 Latex reagent sensitized with hemolytic streptococcal nicotinamide adenine dinucleotidase (nadase), method of producing the same and method of detecting nadase antibody using the same Pending JPS5633550A (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP10910679A JPS5633550A (en) 1979-08-29 1979-08-29 Latex reagent sensitized with hemolytic streptococcal nicotinamide adenine dinucleotidase (nadase), method of producing the same and method of detecting nadase antibody using the same
GB8027769A GB2058083B (en) 1979-08-29 1980-08-28 Composition and method for determining antibody
DE19803032464 DE3032464A1 (en) 1979-08-29 1980-08-28 MEASUREMENT AND METHOD FOR DETERMINING THE ANTIBODY FOR STREPTOCOCCIC NICOTINAMIDADENINDINUCLEOTIDASE

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP10910679A JPS5633550A (en) 1979-08-29 1979-08-29 Latex reagent sensitized with hemolytic streptococcal nicotinamide adenine dinucleotidase (nadase), method of producing the same and method of detecting nadase antibody using the same

Publications (1)

Publication Number Publication Date
JPS5633550A true JPS5633550A (en) 1981-04-04

Family

ID=14501721

Family Applications (1)

Application Number Title Priority Date Filing Date
JP10910679A Pending JPS5633550A (en) 1979-08-29 1979-08-29 Latex reagent sensitized with hemolytic streptococcal nicotinamide adenine dinucleotidase (nadase), method of producing the same and method of detecting nadase antibody using the same

Country Status (3)

Country Link
JP (1) JPS5633550A (en)
DE (1) DE3032464A1 (en)
GB (1) GB2058083B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011515685A (en) * 2008-03-26 2011-05-19 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング Method for long-term storage of substrate-bound beads

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3637253A1 (en) * 1986-11-03 1988-05-05 Behringwerke Ag LATEX AGGLUTINATION METHOD FOR DETECTING ANTI-STREPTOCOCCAL DESOXYRIBONUCLEASE B

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH625626A5 (en) * 1976-03-25 1981-09-30 Hoffmann La Roche Process for the preparation of stable immunological reagents

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011515685A (en) * 2008-03-26 2011-05-19 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング Method for long-term storage of substrate-bound beads

Also Published As

Publication number Publication date
GB2058083A (en) 1981-04-08
GB2058083B (en) 1983-03-02
DE3032464A1 (en) 1981-03-26

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